Physiological and Molecular Plant Pathology最新文献_第5页

Biopesticides for a sustainable agriculture: Prospects and challenges in disease management 促进可持续农业的生物农药：疾病管理的前景与挑战

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2025-12-24 DOI: 10.1016/j.pmpp.2025.103096

Rachid Lahlali , Mohamed Kouighat , Mohammed Khadiri , Abdellatif Boutagayout , Göksel Özer , Salah-Eddine Laasli , Abdelaaziz Farhaoui

{"title":"Biopesticides for a sustainable agriculture: Prospects and challenges in disease management","authors":"Rachid Lahlali , Mohamed Kouighat , Mohammed Khadiri , Abdellatif Boutagayout , Göksel Özer , Salah-Eddine Laasli , Abdelaaziz Farhaoui","doi":"10.1016/j.pmpp.2025.103096","DOIUrl":"10.1016/j.pmpp.2025.103096","url":null,"abstract":"<div><div>The search for new sustainable approaches to crop disease management is fueled by growing ecological and health problems related to the overuse of chemical pesticides and the emergence of compound resistance. Biopesticides, defined as biological control agents derived from living organisms or their metabolites (microorganisms, plants, or natural compounds), offer a potential solution. These biological products can act through safe and targeted modes of action. The taxonomic and functional diversity of biopesticides is first examined in detail in this review, followed by an in-depth study of their molecular mechanisms for pathogen control. To better understand how biopesticides can be improved for enhanced efficacy in the field, we explore recent advances in omics technologies, genetic enhancement, and formulation strategies. The review examines how biopesticides can be integrated into real-world agroecosystems, with a focus on their use in organic farming, integrated disease management, and their interactions with other biological inputs. To improve application uniformity and support the systematic and large-scale integration of biopesticides into agricultural production systems, this review identifies the main existing constraints, regulatory gaps, and future research priorities.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103096"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of thermostable chitinase I168L in the control of anthracnose on avocado and evaluation of cross-infection ability of Colletotrichum species 耐热几丁质酶I168L在牛油果炭疽病防治中的应用及炭疽菌交叉侵染能力评价

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.pmpp.2026.103142

Le Thi Ha Thanh, Dang Van Thanh, Huynh Thi Thu Ha, Nguyen Hoang Loc

{"title":"Application of thermostable chitinase I168L in the control of anthracnose on avocado and evaluation of cross-infection ability of Colletotrichum species","authors":"Le Thi Ha Thanh, Dang Van Thanh, Huynh Thi Thu Ha, Nguyen Hoang Loc","doi":"10.1016/j.pmpp.2026.103142","DOIUrl":"10.1016/j.pmpp.2026.103142","url":null,"abstract":"<div><div>Anthracnose, induced by Colletotrichum species, represents a severe threat to tropical fruit, particularly during storage, leading to considerable economic losses. The objective of this study was to assess the efficacy of the enzyme I168L, a chitinase mutant obtained from <em>Trichoderma asperellum</em> SH16, in the prevention and treatment of avocado anthracnose caused by <em>C. endophyticum</em> AD24.3. The study results revealed that among the three tested temperatures (28, 32, and 35 °C), <em>C. endophyticum</em> AD24.3 demonstrated strong growth at 28 °C. The introduction of 167 × 10<sup>−8</sup> kat of the I168L enzyme significantly hindered the growth of <em>C. endophyticum</em> AD24.3 after 7 days at both concentrations of 10<sup>5</sup> and 10<sup>6</sup> conidia/mL in preventive and curative experiments. Cross-infection trials showed that <em>C. siamense</em> CH26.1, which was isolated from chili, did not induce anthracnose symptoms in avocados at temperatures of 28 °C and 32 °C. Conversely, <em>C. laticiphilum</em> MC81, known to infect mangoes, was able to infect avocados; however, the application of 167 × 10<sup>−8</sup> kat of the I168L enzyme effectively suppressed the growth of this pathogen at 32 °C. These findings in the present study strongly suggest the potential of the I168L enzyme as a promising biocontrol agent for effectively managing avocado anthracnose disease caused by <em>C. endophyticum</em> AD24.3.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103142"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morpho-molecular identification of Phytophthora palmivora and Lasiodiplodia theobromae associated with cacao pod rot in South Sulawesi, Indonesia 印尼南苏拉威西地区与可可豆荚病相关的棕榈疫霉和可可枯霉形态分子鉴定

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2026-02-04 DOI: 10.1016/j.pmpp.2026.103119

Eirene Brugman , Nur Hardina , Nurmujahidin , M. Bayu Mario

{"title":"Morpho-molecular identification of Phytophthora palmivora and Lasiodiplodia theobromae associated with cacao pod rot in South Sulawesi, Indonesia","authors":"Eirene Brugman , Nur Hardina , Nurmujahidin , M. Bayu Mario","doi":"10.1016/j.pmpp.2026.103119","DOIUrl":"10.1016/j.pmpp.2026.103119","url":null,"abstract":"<div><div>Cacao pod rot is one of the most destructive diseases limiting cacao production worldwide, predominantly caused by <em>Phytophthora palmivora</em>. However, field observations in South Sulawesi, Indonesia, suggested the involvement of other fungi in association with pod rot symptoms. This study aimed to identify and characterize the pathogen species associated with pod rot disease on cacao and to assess their pathogenicity through detached pod assays. Isolation and morphological characterization of isolates from symptomatic pods revealed three genera: <em>Phytophthora</em>, <em>Lasiodiplodia</em>, and <em>Fusarium</em>. Pathogenicity tests showed that only <em>P. palmivora</em> and <em>L. theobromae</em> induced pod rot symptoms, whereas <em>Fusarium</em> sp. was non-pathogenic under the test conditions. Symptom development assays demonstrated that infection by <em>P. palmivora</em> occurred rapidly, producing brown necrotic lesions and white mycelial growth, while <em>L. theobromae</em> infection developed more slowly and was accompanied by gray mycelium and black conidiophore masses. Phylogenetic analyses based on the ITS and <em>tef1-α</em> regions confirmed the isolates as <em>P. palmivora</em> and <em>L. theobromae</em>. These findings demonstrate that <em>P. palmivora</em> remains the primary causal agent of cacao pod rot in South Sulawesi, while <em>L. theobromae</em> can act as an opportunistic pathogen associated with secondary colonization and tissue deterioration.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103119"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis MYB12 overexpression modulates flavonoid levels and confers enhanced fungal resistance in plants 拟南芥MYB12过表达调节类黄酮水平，增强植物的真菌抗性

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1016/j.pmpp.2026.103131

Hiteshwari Sinha , Tushita Rai , Ravi Shankar Kumar , Tapasya Datta , Suchi Srivastava , Akanksha Singh , Prabodh Kumar Trivedi

{"title":"Arabidopsis MYB12 overexpression modulates flavonoid levels and confers enhanced fungal resistance in plants","authors":"Hiteshwari Sinha , Tushita Rai , Ravi Shankar Kumar , Tapasya Datta , Suchi Srivastava , Akanksha Singh , Prabodh Kumar Trivedi","doi":"10.1016/j.pmpp.2026.103131","DOIUrl":"10.1016/j.pmpp.2026.103131","url":null,"abstract":"<div><div>Plants produce a wide variety of low-molecular-weight secondary metabolites crucial for development, regulation, and stress adaptation. Flavonoids, a major class among these compounds, are well known for their roles in antioxidant defense and immune responses in plants. Their biosynthesis is primarily controlled by R2R3-MYB transcription factors, with MYB12 acting as a key positive regulator. While MYB12's involvement in flavonoid production and pest resistance has been studied, its role in fungal stress responses remains poorly understood. In this study, we demonstrate that AtMYB12 is significantly upregulated in response to fungal infection by <em>Rhizoctonia solani AG-1 IA</em>. Heterologous expression of <em>AtMYB12</em> in tomato, as well as overexpression in <em>Arabidopsis thaliana</em>, resulted in elevated flavonoid accumulation and enhanced expression of defense-associated genes, thereby mitigating the detrimental effects caused by <em>R. solani AG-1 IA</em> infection. Conversely, <em>myb12</em> single mutants and <em>myb11/12/111</em> triple mutants exhibited reduced flavonoid content and compromised defense responses against fungal infection. These findings provide valuable insights into MYB12-mediated regulation of flavonoid biosynthesis and highlight its essential role in enhancing biotic stress tolerance in plants.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103131"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthracnose on Dioscorea nipponica caused by Colletotrichum spaethianum newly reported in China 中国新报道的日本薯蓣炭疽病

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2025-11-29 DOI: 10.1016/j.pmpp.2025.103071

Yuting Li , Jiaju Wu , Songhan Han , Lili Zhang , Naibo Yang , Yufei Chen

{"title":"Anthracnose on Dioscorea nipponica caused by Colletotrichum spaethianum newly reported in China","authors":"Yuting Li , Jiaju Wu , Songhan Han , Lili Zhang , Naibo Yang , Yufei Chen","doi":"10.1016/j.pmpp.2025.103071","DOIUrl":"10.1016/j.pmpp.2025.103071","url":null,"abstract":"<div><div>Throughhill yam (<em>Dioscorea nipponica</em> Makino), traditionally known as ‘Chuandilong’ in Chinese, is a perennial medicinal plant widely used in traditional Chinese medicine (TCM). Anthracnose, caused by <em>Colletotrichum</em> species, poses a significant threat to the Chinese herbal medicine industry. In June 2024, we conducted a survey of <em>D. nipponica</em> anthracnose disease throughout Hailin county Heilongjiang Province and collected 18 <em>Colletotrichum</em> isolates. Based on morphological traits and sequence analyses of multiple genes including internal transcribed spacer region of ribosomal DNA, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase, actin, and beta-tubulin, the isolates were identified as <em>Colletotrichum spaethianum</em>. Pathogenicity tests on <em>D. nipponica</em> leaves revealed that the tested isolates could induce foliar disease symptom. To the best of our knowledge, this is the first report of <em>C</em>. <em>spaethianum</em> as the causal agent of anthracnose on <em>D</em>. <em>nipponica</em> in China.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103071"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome of Bacillus velezensis HT_B8 unravels endophytic strategies and biocontrol potential for grapevine sustainability 葡萄芽孢杆菌HT_B8基因组揭示葡萄可持续性的内生策略和生物防治潜力

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2026-01-28 DOI: 10.1016/j.pmpp.2026.103120

Hiba Trabelsi , Ghedira kais , Oussema Khamessi , Synda Chenenaoui , Sabrine Hdira , Ghada Mahjoub , Rihab Mahjoub , Hassen Zemni , Ameni Ben Alaya , Mohsen Hanana , Yosr Zaouali , Vincenzo Mondello , Asma Bensalem , Chokri Messaoud , Florence Fontaine , Asma Ben Ghnaya-Chakroun

{"title":"Genome of Bacillus velezensis HT_B8 unravels endophytic strategies and biocontrol potential for grapevine sustainability","authors":"Hiba Trabelsi , Ghedira kais , Oussema Khamessi , Synda Chenenaoui , Sabrine Hdira , Ghada Mahjoub , Rihab Mahjoub , Hassen Zemni , Ameni Ben Alaya , Mohsen Hanana , Yosr Zaouali , Vincenzo Mondello , Asma Bensalem , Chokri Messaoud , Florence Fontaine , Asma Ben Ghnaya-Chakroun","doi":"10.1016/j.pmpp.2026.103120","DOIUrl":"10.1016/j.pmpp.2026.103120","url":null,"abstract":"<div><div>Grapevine trunk diseases (GTDs), including Botryosphaeria dieback, severely reduce grapevine yield and vineyard longevity worldwide. <em>Bacillus velezensis</em> HT_B8, an endophytic bacterium isolated from <em>Vitis vinifera</em> cv. Muscat of Italy in Bouargoub (Nabeul, Tunisia), efficiently colonizes grapevine tissues and strongly inhibits major GTD pathogens. Its draft genome is 4,066,534 bp (GC 45 %) and encodes 3848 proteins, 84 tRNAs, and 3 rRNA operons. Genome analysis revealed numerous biosynthetic gene clusters for antimicrobial secondary metabolites, including bacillaene, difficidin, surfactin, and fengycin, as well as genes linked to plant-microbe interactions such as motility, biofilm formation, siderophore production, and auxin biosynthesis. Comparative genomics identified unique genes potentially enhancing biocontrol efficacy, consistent with <em>in vitro</em> assays showing potent antifungal activity. These results provide insights into the genetic determinants of HT_B8's biocontrol potential and support its use as a sustainable strategy to manage grapevine trunk diseases.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103120"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of new isolates of Puccinia polysora and their interactions with RppC and RppK in maize 玉米多孢契诃子新分离株的鉴定及其与RppC和RppK的相互作用

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2025-12-10 DOI: 10.1016/j.pmpp.2025.103082

Huijin Sun , Xinxing Li , Yufan Wang , Xincao Li , Zixuan Wang , Huanying Li , Javed Hussian Sahito , Jihuan Tang , Ce Deng , Junqiang Ding

{"title":"Identification of new isolates of Puccinia polysora and their interactions with RppC and RppK in maize","authors":"Huijin Sun , Xinxing Li , Yufan Wang , Xincao Li , Zixuan Wang , Huanying Li , Javed Hussian Sahito , Jihuan Tang , Ce Deng , Junqiang Ding","doi":"10.1016/j.pmpp.2025.103082","DOIUrl":"10.1016/j.pmpp.2025.103082","url":null,"abstract":"<div><div>Southern corn rust (SCR), caused by <em>Puccinia polysora</em> (<em>P</em>. <em>polysora</em>), poses a serious threat to maize production. To combat SCR, resistance (<em>R</em>) genes have been widely utilized in maize breeding programs. However, the resistance conferred by these <em>R</em> genes has been declining due to the rapid variation of <em>P</em>. <em>polysora</em> races. To understand the diversity and variation of <em>P</em>. <em>polysora</em> races, we screened for new isolates using differential hosts, in addition to the three previously reported isolates (PP.CN1.0 to PP.CN3.0), we identified and molecularly characterized two new isolates (PP.CN4.0 and PP.CN5.0), and analysed the resistance spectra of two <em>R</em> genes (<em>RppC</em> and <em>RppK</em>) against these new isolates as well as the three previously reported ones. We found that <em>RppC</em> exhibits high resistance to PP.CN4.0 and PP.CN5.0, whereas <em>RppK</em> shows intermediate resistance to PP.CN4.0 and susceptibility to PP.CN5.0. Phylogenetic analysis revealed that the five isolates (PP.CN1.0 to PP.CN5.0) cluster into three groups, with the two new isolates (PP.CN4.0 and PP.CN5.0) forming a distinct group. Our research clarifies the resistance spectra of <em>RppC</em> and <em>RppK</em> against the five <em>P. polysora</em> isolates (PP.CN1.0 to PP.CN5.0) and highlights the emergence of new isolates that can overcome <em>R</em> gene-mediated resistance in maize.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103082"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145796968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An update to the molecular identification of Xanthomonas campestris disease causing pathogens in crucifers – A mini review 十字花科植物油菜黄单胞菌病原菌分子鉴定进展综述

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2025-12-26 DOI: 10.1016/j.pmpp.2025.103102

Nihar Sahu , Masao Watanabe , Jong-In Park

{"title":"An update to the molecular identification of Xanthomonas campestris disease causing pathogens in crucifers – A mini review","authors":"Nihar Sahu , Masao Watanabe , Jong-In Park","doi":"10.1016/j.pmpp.2025.103102","DOIUrl":"10.1016/j.pmpp.2025.103102","url":null,"abstract":"<div><div>Diseases like black rot, leafspot, and bacterial blight caused by <em>Xanthomonas campestris</em> pathovars are serious threat to the <em>Brassica</em> production. Management of the disease using chemical methods is effective but often expensive and environmentally harmful. Breeding resistant varieties offers a cost-effective and eco-friendly alternative, but the presence of diverse races, each exhibiting varied resistance across different hosts, further complicates disease management. Furthermore, the introduction of new races of plant pathogens into new regions through international seed trade presents an additional challenge. Understanding the diversity of pathogen races in a region is essential for developing effective breeding programs. Although conventional detection methods are accurate, they usually take longer time. Therefore, developing molecular markers for the early and specific identification of <em>X. campestris</em> pathovars and their races is critical. With the development of technologies like genome sequencing it is now easier to compare the genomes sequences of closely related species of plant pathogens and identify the species-specific or race-specific regions for marker development. This review presents the works done on the molecular identification of <em>X. campestris</em> causing diseases in crucifers.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103102"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A low pH level in ripening pear tissue promotes the growth and pathogenicity of Alternaria alternata by causing ammonia accumulation and alkalization 梨成熟组织中的低pH水平通过引起氨积累和碱化促进交替病菌的生长和致病性

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2025-11-25 DOI: 10.1016/j.pmpp.2025.103050

Meiqi Gao, Rong Li, Yanxia Zhang, Puwei Yan, Xiwang Wang, Yue Xie, Miao Zhang, Yongcai Li

{"title":"A low pH level in ripening pear tissue promotes the growth and pathogenicity of Alternaria alternata by causing ammonia accumulation and alkalization","authors":"Meiqi Gao, Rong Li, Yanxia Zhang, Puwei Yan, Xiwang Wang, Yue Xie, Miao Zhang, Yongcai Li","doi":"10.1016/j.pmpp.2025.103050","DOIUrl":"10.1016/j.pmpp.2025.103050","url":null,"abstract":"<div><div>The pH value of ripening pear tissues is relatively low, ranging from 4.0 to 5.0. However, the alkalinizing fungus <em>Alternaria alternata</em>, can easily cause black spot during post-harvest storage. To elucidate its potential pathogenic mechanisms, we investigated the effect of low pH in pear tissues on the alkalinization and pathogenicity of <em>A. alternata</em> through in <em>vivo</em> and in <em>vitro</em> test. The results showed that the optimal growth pH for <em>A. alternata</em> was 8, and an alkaline environment enhanced the activity of cell-wall-degrading enzymes (CWDEs) produced by <em>A. alternata</em> thereby increasing its pathogenicity on pears. The low pH (4–4.5) environment of simulated pear juice and pear tissue promoted the pH in the growth medium of <em>A. alternata</em> to increase by 2.7 and 2.235 units, respectively, and the accumulation of ammonia reached 2.134 μmol/mL and 1.63 μmol/g. Further investigations revealed that the pH-dependent signaling pathway involving the Pal protein, the core transcription factor PacC and genes related to ammonia metabolism and secretion (<em>AaGDH2</em>, <em>AaAMET</em> and <em>AaMEPB</em>) were all temporally up-regulated during alkalinization induced by low pH. In particular, <em>AaAMET</em> and <em>AaMEPB</em> increased by 4.05 times and 12.74 times at 96 h compared with 24 h, respectively. These findings suggest that <em>A. alternata</em> can sense low pH in pear tissue through a pH-dependent signaling pathway, facilitating its ammonia accumulation and alkalinization of the environment to promote its growth and pathogenicity.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"142 ","pages":"Article 103050"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of ethanol and Streptomyces angustmyceticus NR8-2 cell-free filtrate against Phytophthora palmivora causing durian stem rot 乙醇和苦瓜链霉菌NR8-2无细胞滤液对引起榴莲茎腐病的棕榈疫霉的协同作用

IF 3.3 3区农林科学

Physiological and Molecular Plant Pathology Pub Date : 2026-03-01 Epub Date: 2025-11-25 DOI: 10.1016/j.pmpp.2025.103046

Prisana Wonglom , Nutthachai Pongprasert , Samak Kaewsuksaeng , Anurag Sunpapao

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