与野花扁豆病（Allmania nodiflora）表型和绿化相关的“澳洲候选植物原体”菌株首次报道

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-06-06 DOI:10.1016/j.pmpp.2025.102777

Natesan Boopathi, Gandhi Karthikeyan, R.K. Mohana Pradeep, Kathiresan Sivagnananpazham

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引用次数: 0

摘要

2025年2月，在印度泰米尔纳德邦塞勒姆的一个花生生态系统中，发现了疑似植物原体的绿化和叶状学症状。为了研究植物原体是否存在，我们从有症状和无症状的植物组织中提取了总DNA，并使用植物原体通用引物进行巢式PCR检测，扩增子长度为1.2 kbp。secA基因也通过半巢式PCR扩增，产生约480 bp的扩增子。序列分析表明，16S rRNA和secA基因序列与“Candidatus Phytoplasma australasiatium”（16SrII-D）亚群具有99% ~ 100%的同源性。本研究提出了“Ca”的第一个宿主报告。澳洲假单胞菌侵染植物。

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First report of ‘Candidatus Phytoplasma australasiaticum’ strain associated with phyllody and virescence of Allmania nodiflora

Suspected phytoplasma symptoms of virescence and phyllody were noticed on Allmania nodiflora (node flower) plants in a groundnut ecosystem located at Salem, Tamil Nadu, India during February 2025. To investigate the possible presence of phytoplasmas, total DNA was extracted from symptomatic and asymptomatic plant tissues and further subjected to nested PCR assays with phytoplasma universal primers which produced an amplicon of 1.2 kbp. The secA gene was also amplified by using semi-nested PCR assay which generated an amplicon of ∼480 bp. The sequence analysis revealed that the 16S rRNA and secA gene sequences shared 99–100 % identity with strains of ‘Candidatus Phytoplasma australasiaticum’ (16SrII-D) subgroup. This study presents the first host report of ‘Ca. P. australasiaticum’ infecting A. nodiflora plants.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.