Antifungal potential of non-ribosomal peptide producing Serratia surfactantfaciens (S31) and Alcaligenes pakistanensis (S33) isolated from the Solanum lycopersicum rhizosphere

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-06-07 DOI:10.1016/j.pmpp.2025.102778

Aetsam Bin Masood , Malik Badshah , Muhammad Ishtiaq Ali , Khadija Masood , Asif Jamal , Mogens Nicolaisen

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引用次数: 0

Abstract

Non-ribosomal peptide (NRP) producing bacteria confer promising alternatives for control of plant pathogens. In the current study Serratia surfactantfaciens (S31) and Alcaligenes pakistanensis (S33) were isolated from the rhizosphere of Solanum lycopersicum. The main aim of the study was to explore the antifungal potential of these novel strains, to characterize the synthesized NRPs and determine their antibiosis mechanisms and influence on plant growth. To screen the strains for their antifungal potential, dual assays were performed against the plant pathogens Aspergillus niger, A. flavus, A. fumigatus and Fusarium oxysporum. When screened for quantitative results regarding enzyme production, A. pakistanensis exhibited a zone of 38.50 ± 4.95 mm for pectinase whereas S. surfactantfaciens showed a zone of 23.0 ± 2.82 mm for cellulase. Antifungal assays showed prominent zones of growth inhibition of the phytopathogens using both bacterial cultures and cell-free supernatants. Genetic analysis revealed the presence of various NRP genes in the bacterial genomes, which were further confirmed by structural analysis. In vivo experimentation showed higher seedling vigour and plant growth parameters when grown in autoclaved as compared to non-autoclaved soil for the applied treatments.

查看原文本刊更多论文

番茄茄根际非核糖体肽产生表面活性剂沙雷氏菌（S31）和巴基斯坦碱性菌（S33）的抗真菌潜力

产生非核糖体肽（NRP）的细菌为控制植物病原体提供了有前途的选择。本研究从番茄茄根际分离得到表面活性剂沙雷氏菌（S31）和巴基斯坦碱性菌（S33）。本研究的主要目的是探索这些新菌株的抗真菌潜力，对合成的nrp进行表征，并确定其抗菌机制和对植物生长的影响。为了筛选菌株对植物病原菌黑曲霉、黄曲霉、烟曲霉和尖孢镰刀菌的抑菌能力。当筛选酶产量的定量结果时，a . pakistani ensis的果胶酶区为38.50±4.95 mm，而S. surfactantfaciens的纤维素酶区为23.0±2.82 mm。抗真菌实验表明，使用细菌培养物和无细胞上清液对植物病原菌的生长有明显的抑制作用。遗传分析显示，细菌基因组中存在多种NRP基因，并通过结构分析进一步证实了这一点。体内试验结果表明，与非蒸压土壤相比，在蒸压土壤中生长的幼苗活力和植株生长参数更高。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.