双dsrna靶向协同沉默Nox1和MoREI1可阻断水稻Magnaporthe oryzae ros依赖性入侵

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-09-05 DOI:10.1016/j.pmpp.2025.102915

Gengxuan Yan , Shuang Li , Yuan Tian , Wenjing Duan , Meng Sun , Lu Zhou , Jialing Zhang , Haihua Xia , Chong Yu , Shumei Zhang , Zixuan Wang , Hongyi Yang

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引用次数: 0

摘要

稻瘟病是由稻瘟病菌（M. oryzae）引起的稻瘟病，严重威胁着全球水稻生产。我们探索了针对关键真菌基因的外源性RNAi作为化学杀菌剂的环保替代品。荧光检测证实了dsRNA内在化到菌丝中。我们以100 ng/ μL-a的剂量效应试验优化的dsRNAs靶向Nox1（参与ros介导的渗透）和MoREI1（效应相关）。这导致真菌在体外（Nox1-dsRNA菌落直径减少37.7%）和离体叶片上的生长显著减少（病变长度减少90%）。全株试验显示了协同效应：双dsrna处理使相对病变面积减少了72.4%，优于单基因处理。qPCR证实了靶转录物的沉默（Nox1的沉默率约为80%）。叶面施用dsRNA可抑制gfp标记菌株的感染，阻断宿主入侵。我们的研究在m.o ryzae中建立了有效的RNAi，并强调了双基因靶向在可持续的blast管理中的潜力。

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Synergistic silencing of Nox1 and MoREI1 by dual-dsRNA targeting blocks Magnaporthe oryzae ROS-dependent invasion in rice

Rice blast, caused by Magnaporthe oryzae (M. oryzae), severely threatens global rice production. We explored exogenous RNAi targeting key fungal genes as an eco-friendly alternative to chemical fungicides. Fluorescence-based assays confirmed the internalization of dsRNA into the hyphae. We applied dsRNAs targeting Nox1 (involved in ROS-mediated penetration) and MoREI1 (effector-related) at 100 ng/μL—a concentration optimized through dose-response tests. This resulted in a significant reduction in fungal growth in vitro (a 37.7 % decrease in colony diameter for Nox1-dsRNA) and on detached leaves (a 90 % reduction in lesion length). Whole-plant assays showed a synergistic effect: dual-dsRNA treatment reduced relative lesion area by 72.4 %, outperforming single-gene treatments. qPCR confirmed the silencing of target transcripts (∼80 % for Nox1). Foliar application of dsRNA suppressed infection by a GFP-tagged strain, blocking host invasion. Our study establishes efficient RNAi in M. oryzae and underscores the potential of dual-gene targeting for sustainable blast management.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.