Pakistan journal of pharmaceutical sciences最新文献_第8页

Combination of acupoint herbal plaster and ear acupuncture for effective pain relief in patients after laparoscopic appendectomy. 穴位药膏配合耳针治疗腹腔镜阑尾切除术后疼痛的疗效观察。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13887.1

Heng Huang, Min Su, Fei Liu, Wenbo Long

{"title":"Combination of acupoint herbal plaster and ear acupuncture for effective pain relief in patients after laparoscopic appendectomy.","authors":"Heng Huang, Min Su, Fei Liu, Wenbo Long","doi":"10.36721/PJPS.2025.38.4.REG.13887.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.13887.1","url":null,"abstract":"Laparoscopic appendectomy effectively treats appendicitis and other related conditions. However, postoperative pain remains a major concern. This study elucidates the effect of traditional Chinese medicine (TCM) therapies on postoperative recovery of patients who had undergone laparoscopic appendectomy. The study encompassed 262 patients, including 41 controls and 221 TCM-treated patients. Quality control was monitored by two senior TCM physicians and one researcher. Postoperative outcomes included the length of hospitalization, antibiotic use, pain, analgesia and plasma drainage. No significant differences were observed between the groups concerning sex, age, acute appendicitis, postoperative complications and plasma drainage, except for pain and analgesia (both P<0.001). Moxibustion or partitioned moxibustion alone had no effect, compared with the control; however, ear acupuncture alone, acupoint herbal plaster + ear acupuncture, acupoint herbal plaster + moxibustion/partitioned moxibustion and acupoint herbal plaster + ear acupuncture + moxibustion/partitioned moxibustion exerted a significant effect. Pain index and analgesia duration were significantly lower for ear acupuncture alone than in the control group but remained higher than in the three acupoints herbal plaster-treated groups. The combination of acupoint herb plaster and ear acupuncture provided greater pain relief than ear acupuncture alone.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1398-1414"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation development and pharmacokinetic evaluation of celecoxib loaded hydroxyl propyl methyl cellulose (HPMC) microparticles in in-vivo model. 塞来昔布负载羟丙基甲基纤维素（HPMC）微粒体内模型的配方开发及药动学评价。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.14538.1

Khuram Shahazad, Syed Umer Jan, Rahman Gul, Mahjabeen Tafazzul, Safia Mengal, Falsafa Jamal, Nayab Iqbal

{"title":"Formulation development and pharmacokinetic evaluation of celecoxib loaded hydroxyl propyl methyl cellulose (HPMC) microparticles in in-vivo model.","authors":"Khuram Shahazad, Syed Umer Jan, Rahman Gul, Mahjabeen Tafazzul, Safia Mengal, Falsafa Jamal, Nayab Iqbal","doi":"10.36721/PJPS.2025.38.4.REG.14538.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.14538.1","url":null,"abstract":"This investigation aimed to formulate Celecoxib-loaded microparticles for targeted delivery to the colon, employing Hydroxypropylmethylcellulose (HPMC). Celecoxib's effectiveness in managing colorectal cancer (CRC)-related pain and inflammation is compromised by its low solubility and gastrointestinal side effects. A total of seventeen formulations were synthesized through emulsion solvent evaporation (ESE) oil-in-oil technique. The physicochemical properties of the formulations were analyzed through Fourier Transform Infrared Spectroscopy, X-ray Diffraction, Scanning Electron Microscopy and Differential Scanning Calorimetry; however, rabbits served as the biological specimens in the in vivo pharmacokinetic evaluation in Rabbits plasma. Celecoxib pure drug, F1 and F2 were given to the rabbits one time dose to assess the pharmacokinetics evaluation. The average particle size for F 1 was 497.93μm and 497.93 μm for F 2. In vitro showed cumulative release rates of 88.77% for F1 and 88.85% F2 after 24 hours. FTIR analysis confirmed the compatibility of the formulation ingredients, XRD revealed a transition of Celecoxib from its crystalline to amorphous form, DSC indicated the formulations' thermal stability and SEM images showed dense, spherical microparticles. Given these factors, using HPMC micro particles to carry Celecoxib could enhance the drug delivery and released in the body and may result effective treatment with fewer side effects.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1470-1483"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on the medical safety of botanical medicines: Novel toxic metals and microbial contamination approach. 植物性药物的医疗安全性研究：新型有毒金属和微生物污染方法。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.14088.1

Roqayah Hassan Kadi, Uguru Hilary, Akpokodje Ovie Isaac, Idama Omokaro, Rokayya Sami, Suha Abduljawad, Fayez Alsulaimani, Ahmed Basri, Ola Abu Ali, Mahmoud Helal

{"title":"Studies on the medical safety of botanical medicines: Novel toxic metals and microbial contamination approach.","authors":"Roqayah Hassan Kadi, Uguru Hilary, Akpokodje Ovie Isaac, Idama Omokaro, Rokayya Sami, Suha Abduljawad, Fayez Alsulaimani, Ahmed Basri, Ola Abu Ali, Mahmoud Helal","doi":"10.36721/PJPS.2025.38.4.REG.14088.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.14088.1","url":null,"abstract":"The utilization of herbal therapies is increasing globally, prompting investigations into the health complications that may arise from drug side effects. A total of 160 herbal medicines (HMs), were used for this study, and their toxic heavy metal content, microbial load and dielectric properties were evaluated. The results obtained revealed that the crude HMs exhibited a greater proportion of heavy metals, microorganisms and a higher dielectric constant (ɛ') value, in comparison to the refined HMs. Particularly, the powdered HMs contained high heavy metals and microbial contaminations. For the unrefined powders, HM samples exceeded the safety limits of cfu/g ≤ 105, approved by World Health Organization for herbal medications. Regarding the electrical properties, the ɛ' for the crude unrefined HMs ranged from 9.19 to 92.67; while the refined HMs ɛ' values varied from 7.35 to 39.15. The hazard index (HI) analysis showed that unrefined HMs have higher non-carcinogenic toxicity than refined ones, with children facing serious health risks as their HI values surpass 1. Crude heavy metal consumption increases a child's risk of developing cancer. This research's findings highlighted the importance of consistent monitoring of herbal products, as heavy metals can accumulate, resulting in toxicity after prolonged use of these drugs.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1389-1397"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of metformin on the motor activity and hippocampal dopamine metabolism in rats subjected to repeated immobilization stress. 二甲双胍对反复固定应激大鼠运动活动及海马多巴胺代谢的影响。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.12964.1

Rushda Afroz, Huma Ikram, Shazia Nawaz, Nazish Mustafa, Tabinda Salman, Darakhshan Jabeen Haleem

{"title":"The effect of metformin on the motor activity and hippocampal dopamine metabolism in rats subjected to repeated immobilization stress.","authors":"Rushda Afroz, Huma Ikram, Shazia Nawaz, Nazish Mustafa, Tabinda Salman, Darakhshan Jabeen Haleem","doi":"10.36721/PJPS.2025.38.4.REG.12964.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.12964.1","url":null,"abstract":"The present study investigates the impact of metformin treatment on motor activity and hippocampal dopamine metabolism in rats subjected to recurring immobilization stress. The experiment was conducted on male Albino Wistar rats, which have been exposed to repeated immobilization stress (2hrs; for 6 days) and administered with metformin on the dose of 50mg/kg. Motor activities were assessed in open field, dopamine and its metabolites were estimated in the hippocampal samples by high performance liquid chromatography with electrochemical detection (HPLC-EC). Biochemical parameters were monitored in serum (serum leptin, serum tryptophan, serum glucose, serum corticosterone, serum cholesterol) and brain samples (hippocampal tryptophan). The results revealed that metformin management mitigated the deleterious consequences of repeated immobilization stress on motor activities of the rats. Furthermore, metformin treatment was associated with alterations in dopamine metabolites in the hippocampus, suggesting possible modulatory role of dopaminergic neurotransmission in response to immobilization stress. These findings contribute to the knowledge of neurobiological mechanisms underlying the therapeutic effects of metformin in stress-associated situations, underscoring its capability as a pharmacological intervention for stress-triggered alterations in motor function and dopaminergic neurotransmission.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1426-1434"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination therapy in pediatric bronchitis: Examining mucolytic, bronchodilator and anti-inflammatory drug effects on secretion management and recovery time. 小儿支气管炎的联合治疗：检查溶粘剂、支气管扩张剂和抗炎药物对分泌物管理和恢复时间的影响。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.14051.1

Xuan Zhang, Xiaoju Xiong, Huiyun Zhu

{"title":"Combination therapy in pediatric bronchitis: Examining mucolytic, bronchodilator and anti-inflammatory drug effects on secretion management and recovery time.","authors":"Xuan Zhang, Xiaoju Xiong, Huiyun Zhu","doi":"10.36721/PJPS.2025.38.4.REG.14051.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.14051.1","url":null,"abstract":"This study investigated the effectiveness of combining mucolytic agents (ambroxol hydrochloride), bronchodilators (terbutaline sulfate), and anti-inflammatory drugs (budesonide) in treating pediatric bronchitis. From September 2023 to February 2024, 410 pediatric patients were divided into two groups: A control group treated with bronchodilators and anti-inflammatory drugs, and a study group receiving the combined therapy. The results indicated that the study group exhibited significantly better clinical efficacy, with faster resolution of symptoms such as dyspnea, cough, and fever compared to the control group (P<0.05). Pulmonary function indicators, including tidal volume and respiratory ratios, also showed greater improvement in the study group (P<0.05). Additionally, serum levels of inflammatory markers (WBC, CRP, TNF-α, IL-6) were lower in the study group after treatment (P<0.05). Sleep quality improved more in the study group, with longer sleep duration and fewer awakenings (P<0.05). Airway secretion clearance function was also better in the study group, with lower sputum volume and viscosity (P<0.05). No significant difference in adverse reactions was observed between the groups (P>0.05). The findings suggest that the combined therapy of mucolytic agents, bronchodilators and anti-inflammatory drugs is highly effective in treating pediatric bronchitis, offering significant benefits in symptom improvement, pulmonary function enhancement, inflammatory reduction, sleep improvement, and airway clearance.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1221-1230"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meta-analysis of the clinical efficacy of various medications for treating pregnancy-induced hypertension. 各种药物治疗妊高征临床疗效的meta分析。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.12626.1

Gaixia Ma, Peng Hu

{"title":"Meta-analysis of the clinical efficacy of various medications for treating pregnancy-induced hypertension.","authors":"Gaixia Ma, Peng Hu","doi":"10.36721/PJPS.2025.38.4.REG.12626.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.12626.1","url":null,"abstract":"To analyse the literature on the efficacy and safety of medications for treating pregnancy-induced hypertension (PIH) and to provide evidence-based recommendations. PubMed, Embase and Web of Science databases were searched for studies published between January 2000 and October 2023. Studies comparing common PIH treatment drugs, either as the experimental group with an untreated control group or as a control group treated conventionally with magnesium sulphate alone or in combination with other drugs, were included in this analysis. The outcome measures assessed were overall treatment efficacy, adverse reactions and postpartum haemorrhage. The quality of the selected studies was evaluated using the Jadad scale and Cochrane risk-of-bias assessment tool. Medications significantly increased treatment efficacy (relative risk [RR] = 2.50, 95% CI = 1.85-3.37, P < 0.00001), reduced adverse reactions (RR = 0.52, 95% CI = 0.38-0.69, P < 0.0001) and decreased the risk of postpartum haemorrhage by 43% (95% CI = 0.35-0.69; P < 0.0001). Common treatment medications significantly increased the overall efficacy of PIH treatment and reduced the incidence of adverse reactions compared with the control treatments. Additionally, these medications reduced the risk of postpartum haemorrhage.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1165-1173"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of extensively drug-resistant Salmonella serovar Typhi in patients with gastrointestinal complications in Quetta, Pakistan. 巴基斯坦奎达胃肠并发症患者中广泛耐药伤寒沙门氏菌的分子特征

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.14048.1

Hafsah Tariq, Abdul Samad, Saadullah Jan, Hafiz Muhammad Mazhar Asjad, Jawad Akbar Khan, Farah Sabeen Bugti, Muhammad Naeem, Abdul Rahim Bugti, Adnan Ayoub

{"title":"Molecular characterization of extensively drug-resistant Salmonella serovar Typhi in patients with gastrointestinal complications in Quetta, Pakistan.","authors":"Hafsah Tariq, Abdul Samad, Saadullah Jan, Hafiz Muhammad Mazhar Asjad, Jawad Akbar Khan, Farah Sabeen Bugti, Muhammad Naeem, Abdul Rahim Bugti, Adnan Ayoub","doi":"10.36721/PJPS.2025.38.4.REG.14048.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.14048.1","url":null,"abstract":"Extensive drug resistance (XDR) S. typhi have been evaluated in patients with gastrointestinal disturbance who attended multiple tertiary care hospitals in Quetta, Balochistan, Pakistan. Blood samples of total of 480 patients were obtained and S. typhi was isolated and verified by PCR. Isolates were subjected to antimicrobial susceptibility testing and antimicrobial resistance (AMR) genes of 1st, 2nd line antibiotics, 3rd generation cephalosporin and azithromycin were identified by PCR. Among 65 PCR confirmed S. typhi cases, 18(27%) were Multidrug resistance (MDR), 25(38%) XDR, 13 (20%) Extended spectrum β-lactamase (ESBL) and only 4(6%) Azithromycin-resistant XDR S. typhi. The high frequency was observed for the antibiotics-resistant genes catA1, blaTEM-1 (100%), dhfr7 (95%), sul1 (98%), gyrA, gyrB, parC (93%) and qnrS and parE 100% each. The frequency of blaCTX-M-15 and acrB were 78% and 6% respectively. We found high burden of MDR, XDR and ESBLs S. typhi. The AMR genes were similar to those of the regional countries. Azithromycin resistance was low could be a drug of choice against XDR S. typhi in the study area. The study provided the molecular profile of AMR S. typhi in Quetta, capital of Balochistan province of Pakistan.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1354-1367"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of preoperative 0.1% topical nepafenac in the prevention of cystoid macular edema after cataract surgery in diabetic patients. 术前0.1%外用尼泊尼酸预防糖尿病患者白内障术后黄斑囊样水肿的疗效。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13237.1

Maham Sultan, Fawad Rizvi, Zeeshan Kamil, Uzma Naz, Afshan Ali, Hamama Barry

{"title":"Efficacy of preoperative 0.1% topical nepafenac in the prevention of cystoid macular edema after cataract surgery in diabetic patients.","authors":"Maham Sultan, Fawad Rizvi, Zeeshan Kamil, Uzma Naz, Afshan Ali, Hamama Barry","doi":"10.36721/PJPS.2025.38.4.REG.13237.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.13237.1","url":null,"abstract":"This study aims to evaluate the efficacy of preoperative topical 0.1% nepafenac in preventing cystoid macular edema after cataract surgery in diabetic patients in Pakistan. Diabetic patients with normal central macular thickness and significant cataracts at Layton Rahmatullah Benevolent Trust Hospital, Karachi, Pakistan were included from March to August 2023. Patients were divided into two groups: Those receiving 0.1% nepafenac and those not. Efficacy was assessed by comparing central macular thickness. Data were analyzed using IBM SPSS version 27. The study included 96 patients with a mean age of 57.96±3.80 years. In the nepafenac group, central macular thickness measured 241.10 ± 16.07 µm preoperatively, 245.88±19.76 µm at 1 month, 242.79±20.02 µm at 2 months, and 240.77±20.17 µm at 3 months. In the control group, measurements were 249.31±10.96 µm preoperatively, 257.50±13.50 µm at 1 month, 266.08±19.53 µm at 2 months, and 266.96±17.30 µm at 3 months. The nepafenac group showed a significant decrease in central macular thickness, with overall efficacy of 93.8% compared to the control group. A statistically significant difference in central macular thickness was observed between diabetic patients who received nepafenac pre- and postoperatively and those who did not.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1321-1327"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosemary reduced estrogen receptor (ESR1) protein mediated cycle arrest and apoptosis: A potential hormonal dependent anticancer mechanism of the plant in the MCF-7 breast cancer cells. 迷迭香降低雌激素受体（ESR1）蛋白介导的周期阻滞和凋亡：一种潜在的激素依赖的MCF-7乳腺癌细胞的抗癌机制。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13514.1

Ahmed Ah Abdellatif, Abdellatif Bouazzaoui, Mahmoud Zaki El-Readi, Hamdoon A Mohammed, Osamah Al Rugaie, Waad A Samman, Mansour Alsharidah

{"title":"Rosemary reduced estrogen receptor (ESR1) protein mediated cycle arrest and apoptosis: A potential hormonal dependent anticancer mechanism of the plant in the MCF-7 breast cancer cells.","authors":"Ahmed Ah Abdellatif, Abdellatif Bouazzaoui, Mahmoud Zaki El-Readi, Hamdoon A Mohammed, Osamah Al Rugaie, Waad A Samman, Mansour Alsharidah","doi":"10.36721/PJPS.2025.38.4.REG.13514.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.13514.1","url":null,"abstract":"Breast cancer is a global health issue, driving the development of various treatment methods, including anticancer drugs. The unfavorable side effects of these medicines, especially in targeting hormones, have increased interest in naturally occurring therapy options due to their potential for many targets and less side effects. Rosemary (Salvia rosmarinus) extract may treat breast cancer naturally. The study used MCF7 cells and found that the rosemary extract exhibited significant anticancer activity, with an IC50 value of 12.5µg/ml. The anticancer effect of the rosemary extract was primarily mediated by apoptosis, which was induced by a substantial reduction in the expression of the estrogen receptor (ESR1) protein. The downregulation of ESR1 led to G0/G1 cell cycle arrest and apoptosis through the activation of downstream pathways, resulting in increased expression of TP53 and BAX, while reducing Bcl2 levels, ultimately leading to apoptosis. Moreover, the study confirmed the extract's ability to induce apoptosis by demonstrating a noticeable increase in DNA damage in the treated cancer cells, as assessed by DNA fragmentation and comet assay. These results demonstrate rosemary extract's potent breast cancer-fighting properties. The study concludes that rosemary extract has potent anticancer effects on breast cancer cells.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1156-1164"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ED₅₀ of hydromorphone suppressing body movement in artificial abortion: A dose-finding study. 氢吗啡酮抑制人工流产中身体运动的ED50：一项剂量发现研究。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13124.1

Lijun Peng, Lingli Liu

引用次数: 0