Pakistan journal of pharmaceutical sciences最新文献_第7页

Effectiveness and safety assessment of calcium channel blockers compared to beta blockers in patients with angina: An observational study. 钙通道阻滞剂与β受体阻滞剂相比对心绞痛患者的有效性和安全性评估：一项观察性研究。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Shama Abidi, Saira Saeed Khan, Sadaf Naeem, Humera Siddiqui, Sumaira Khadim, Saima Saleem, Saira Erum Ejaz, Syed Ishtiaq Rasool, Syeda Maheen Zahidi

{"title":"Effectiveness and safety assessment of calcium channel blockers compared to beta blockers in patients with angina: An observational study.","authors":"Shama Abidi, Saira Saeed Khan, Sadaf Naeem, Humera Siddiqui, Sumaira Khadim, Saima Saleem, Saira Erum Ejaz, Syed Ishtiaq Rasool, Syeda Maheen Zahidi","doi":"","DOIUrl":"","url":null,"abstract":"Beta blockers (BB) and calcium channel blockers (CCB) are highly effective to suppress angina attacks. Current observational study is designed to investigate the effectiveness of BB, CCB and its combination in angina patients. Angina patients from different tertiary care hospital cardiology OPDs were recruited from June 2022 to June 2023. Patient's history and suspected adverse drug effects (ADE) observed by manual chart review. Results showed baseline demographics and comorbidities were similar. Medication assessment revealed that most patients were on CCB (54.4%) and BB (36.36%) than combination (9.8%). Compared with BB, CCB and combination drugs taking patients represented stable heart rate and blood pressure (P<0.05). There were insignificant differences were observed in electrolytes and lipid profile in each groups. In addition, the Seattle questionnaire for angina (SQA) showed improved symptoms in 83 patients out of 110 (P<0.05). Further ADE were observed by using Naranjo scale that represented BB taking patients were found to have more ADRs than CCB and combination therapy. In conclusion, patients using BB, CCB or a combination of CCB+BB had improved angina symptoms and represented same efficacy however CCB exhibited lesser number of ADRs that shows CCB is more effective than BB in prolong use of angina control.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An efficient RP-HPLC-based approach for simultaneous determination of sitagliptin and metformin HCl in pharmaceutical drug formulation. 基于 RP-HPLC 的高效方法，用于同时测定药物制剂中的西他列汀和盐酸二甲双胍。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Mohsin Ali, Muhammad Raza Shah, Rustem Zairov, Shaista Zafar, Syed Imran Ali, Faizan Ansar, Khadim Mohi Uddin, Nurgali Akylbekov

{"title":"An efficient RP-HPLC-based approach for simultaneous determination of sitagliptin and metformin HCl in pharmaceutical drug formulation.","authors":"Mohsin Ali, Muhammad Raza Shah, Rustem Zairov, Shaista Zafar, Syed Imran Ali, Faizan Ansar, Khadim Mohi Uddin, Nurgali Akylbekov","doi":"","DOIUrl":"","url":null,"abstract":"This study introduces an innovative, and rapid HPLC method using reverse phase elution for the simultaneous analysis of Sitagliptin and Metformin HCl in pharmaceutical formulations. This combination was explored in bulk and solid dosage forms using Luna Phenomenex C8 column (4.6 x 250 mm, 5 µm) at ambient temperature in isocratic elution. It was found that the mobile phase comprising of 0.1% ortho-phosphoric acid, potassium dihydrogen phosphate buffer (pH 3.0) and acetonitrile in ratios 35:35:30, showed a symmetrical peak for Sitagliptin and Metformin HCl. The detection was carried out at 210nm, using a flow rate of 1.0mL/min. The method was linear over the concentration range for Sitagliptin 2.5-7.5 ppm and Metformin HCl 25-75 ppm. The assay recoveries of Sitagliptin and Metformin were found to be 100.36% and 100.20%, respectively. The LOD and LOQ for the Sitagliptin were found to be 0.201 ppm and 0.301 ppm and for Metformin HCl 0.101 ppm and 0.303 ppm, respectively. The proposed methods can be implemented for controlling quality in bulk and solid dosage forms. The analytical methods were validated as per the guideline of ICH Q2 (R2). The developed HPLC methods were effectively employed for the determination of combined dosage forms in pharmaceutical formulations.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic, antioxidant and αlpha-amylase inhibitory activities of wild and Nabali olive leaf extracts from Jordan. 约旦野生橄榄叶和纳巴利橄榄叶提取物的细胞毒性、抗氧化性和α-α-淀粉酶抑制活性。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Maher M Al-Dabbas, Azza Ramadan, Hani J Hamad, Zaher Al-Bashabsheh, Mahmoud Abughoush, Kawthar Kayed, Bha'a Aldin Al-Nawasra, Ahmad Aldabbas, Sehar Iqbal

{"title":"Cytotoxic, antioxidant and αlpha-amylase inhibitory activities of wild and Nabali olive leaf extracts from Jordan.","authors":"Maher M Al-Dabbas, Azza Ramadan, Hani J Hamad, Zaher Al-Bashabsheh, Mahmoud Abughoush, Kawthar Kayed, Bha'a Aldin Al-Nawasra, Ahmad Aldabbas, Sehar Iqbal","doi":"","DOIUrl":"","url":null,"abstract":"Olive leaf extracts contain several phytochemical and pharmacological properties. This study evaluated the cytotoxic, antioxidant, α-amylase inhibitory activities of aqueous, ethanol and ethyl acetate extracts from the Nabali, Muhassan and wild olive leaves grown in Jordan. Total polyphenols, flavonoids and flavonols contents, chelating power activity, total antioxidant activity and DPPH free radical scavenging activity of each extract were evaluated. The α-amylase inhibitory activity of each extract was evaluated using CNP-G3 assay while cytotoxicity was assessed against viability of MCF7 and MB-MDA-231 breast cancer cell lines by MTT. The results showed that total polyphenol content was the highest in the ethanolic wild leaf extract (113.97 mg gallic acid equivalent/g of dry extract). At a concentration of 100µg/ml, the extracts from ethanolic wild leaf, ethyl acetate of wild leaf, and ethanolic Nabali leaf exhibited the highest chelating activity for ferrous ions (52.4%, 50.5%, and 47.2%). All olive leaf extracts significantly reduced MCF7 cell growth, while ethyl acetate wild leaf extract decreased MB-MDA-231 viability. The findings revealed a robust correlation between the antioxidant, cytotoxic and α-amylase inhibitory activities of various olive leaf extracts. Further investigations are needed to identify cytoprotective effects of olive leaf extracts and the evaluation of its efficacy in vivo.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astragalus polysaccharide (APS) improves the immune function in diet-induced obese (DIO) mice. 黄芪多糖（APS）可改善饮食诱导肥胖（DIO）小鼠的免疫功能。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Ziyi Xu, Hongling Xu, Lei Zhang, Liangchun Yan, Jing Zhou, Yang Hong, Jiafei Zhan, Jieying Xia, Tiezhu Chen, Han Dong

{"title":"Astragalus polysaccharide (APS) improves the immune function in diet-induced obese (DIO) mice.","authors":"Ziyi Xu, Hongling Xu, Lei Zhang, Liangchun Yan, Jing Zhou, Yang Hong, Jiafei Zhan, Jieying Xia, Tiezhu Chen, Han Dong","doi":"","DOIUrl":"","url":null,"abstract":"Obesity is a major health hazard, suppressing the immune system and complicating inflammatory symptoms treatment. Traditional Chinese medicine emphasizes holistic principles and syndrome-based diagnosis/therapy. Its primary focus is on enhancing overall well-being, rather than solely aiming for weight loss. Astragalus polysaccharide (APS), extracted from Astragalus membranaceus, has demonstrated promising effects in enhancing the health status of obese individuals. Therefore, this study employed DIO mouse model to explore the immunomodulatory effects of APS in obese mice. The findings revealed a dose-dependent effect of APS on obesity prevention in DIO mice. Specifically, a 4% concentration of APS significantly reduced body weight, whereas a 2% concentration tended to increase it. Furthermore, APS effectively modulated blood glucose and lipid profiles, demonstrating varying degrees of improvement in blood glucose and blood lipid-related factors. Notably, APS also facilitated the reactivation of suppressed immune function in obese mice, regulating a range of immunological variables associated with obesity and thereby maintaining homeostasis. In conclusion, the functional benefits of APS were dose-related, with a 4% concentration demonstrating promising results in obesity prevention and immune system modulation. These findings provide a potential reference for treating inflammatory conditions associated with obesity, contributing academic understanding of obesity management and immunomodulation.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development, synthesis and biological evaluation of imidazole [2,1-b] thiazole derivatives containing an indole ring for their potential anti-inflammatory properties. 含吲哚环的咪唑[2,1-b]噻唑衍生物的开发、合成和生物学评价，以了解其潜在的抗炎特性。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Yu-Cai Jiang, Hai-Feng Lin, Zhi-Wei Chen, Li-Li Zheng

{"title":"Development, synthesis and biological evaluation of imidazole [2,1-b] thiazole derivatives containing an indole ring for their potential anti-inflammatory properties.","authors":"Yu-Cai Jiang, Hai-Feng Lin, Zhi-Wei Chen, Li-Li Zheng","doi":"","DOIUrl":"","url":null,"abstract":"In this study, in order to further search anti-inflammatory drugs with high efficiency and low toxicity, this study took the ring of indoles and imidazole [2,1-b] thiazole as the main skeleton. A total of nine new N-1-substituted derivatives of indole-2-carboxyamide-phenylimidazoles [2,1-b] thiazole (13-20) was synthesized through the processes of cyclization, amino reduction, ester hydrolysis, dehydration condensation and acyl chloride substitution. These derivatives were then tested for their ability to reduce inflammation in RAW 264.7 macrophages. There was a significant majority of these compounds that effectively suppressed the production of NO, IL-6 and TNF-α in RAW 264.7 cells that were stimulated by LPS. One of these compounds, compound 19, was shown to be capable of efficiently lowering the levels of LPS-induced over expression of a number of inflammatory mediators. The inhibition rates for compound 19 were 54.66%, 68.82% and 43.74%, respectively. Additionally, an initial structure-activity relationship evaluation was carried out. The findings indicate that the incorporation of substituted benzyl moieties at the same position provided N-benzylation compounds with a positive anti-inflammatory effect. The electrophilicity of the substituent on the benzyl group had the potential to have an effect on the anti-inflammatory effect, which is something that calls for further investigation.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the mechanism of Cinnamomi Cortex against morphine addiction using network pharmacology and molecular docking analyses. 利用网络药理学和分子对接分析探索 Cinnamomi Cortex 抗吗啡成瘾的机制。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Hancheng Li, Ming Zeng, Yiling Chen, Zhao Jiang, Ziwei Li, Yangkai Wu, Changsheng Liang, Linghong Chen, Chengji Gao, Zhixian Mo

{"title":"Exploring the mechanism of Cinnamomi Cortex against morphine addiction using network pharmacology and molecular docking analyses.","authors":"Hancheng Li, Ming Zeng, Yiling Chen, Zhao Jiang, Ziwei Li, Yangkai Wu, Changsheng Liang, Linghong Chen, Chengji Gao, Zhixian Mo","doi":"","DOIUrl":"","url":null,"abstract":"Cinnamomi Cortex is a commonly used herb with a variety of pharmacological effects. We investigated the molecular mechanisms by which Cinnamomi Cortex antagonises morphine addiction (MA) using network pharmacology and molecular docking techniques in a morphine-dependent rat withdrawal model. The antagonistic effect of Cinnamomi Cortex was observed by inducing withdrawal symptoms in morphine-dependent rats through a dose-escalation method. Network pharmacology and molecular docking techniques were further employed to analyze the substance basis and mechanism of Cinnamomi Cortex in antagonizing MA. Cinnamomi Cortex was screened to contain 10 active ingredients, 127 active targets and 1724 MA-related targets. Among them, 52 targets overlapped between Cinnamomi Cortex and MA and 13 core targets were identified by metric analysis. Cinnamomi Cortex had a significant inhibitory effect on withdrawal symptoms in MA rats, with the most pronounced effect at a moderate dose. The active ingredients of Cinnamomi Cortex (including oleic acid) can act on multiple targets related to MA and regulate multiple pathways to treat MA. The present study reveals the material basis and mechanism of cinnamon's action on MA, and provides insights and references for subsequent experiments exploring the potential therapeutic approach of Cinnamomi Cortex on MA.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro antioxidant and protective effects of the extract of Broussonetia papyrifera leaves on imiquimod-induced skin lesions in psoriasis-like mice. 纸莎草叶提取物对咪喹莫特诱导的银屑病样小鼠皮损的体外抗氧化和保护作用。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Jia An, Ma Xiao-Ying, Huang Xiao-Qiang, Liu Shun-He, Wang Xin, Xiang De-Quan, Xiang Ru-Yi

{"title":"In vitro antioxidant and protective effects of the extract of Broussonetia papyrifera leaves on imiquimod-induced skin lesions in psoriasis-like mice.","authors":"Jia An, Ma Xiao-Ying, Huang Xiao-Qiang, Liu Shun-He, Wang Xin, Xiang De-Quan, Xiang Ru-Yi","doi":"","DOIUrl":"","url":null,"abstract":"Psoriasis is a chronic inflammatory immune-related skin disease. According to literature reports, the leaves of Broussonetia papyrifera exhibit antioxidant, immune-enhancing and antibacterial effects. These leaves are not only used clinically for the treatment of superficial fungal infections and hepatitis, but also used in the development of health food. However, the treatment of psoriasis with the leaves of B. papyrifera has not been reported yet. The in vitro antioxidant activity of B. papyrifera leaf extract was investigated by DPPH, OH and ABTS assays. Furthermore, IMQ was used to induce a mouse psoriasis-like model and HE staining, enzyme-linked immunosorbent assay and biochemical kits were used to measure relevant pathological indexes. The results showed that the B. papyrifera leaf extract has certain antioxidant capacity in vitro, which was positively correlated with its concentration. In addition, the extract can not only improve IMQ-induced skin damage on the back of mice, inhibit TNF-α and IL-6 factor secretion, but also regulate activity of SOD and the serum levels of MDA. Its mechanism of action related to inhibiting the secretion of inflammatory factors and the regulation of oxidative stress in vivo.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastro protective natural polymer aided triple drug therapy to eradicate Helicobacter pylori. 胃保护性天然聚合物辅助三联药物疗法根除幽门螺旋杆菌。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Rajeshpavan Ampapuram, Gopinath Subramaniyan, Ramakrishnan Subburaya Rajendran, Ramakrishna Reddy Padamala

引用次数: 0

Anticancer effects of tanshinone IIA in bladder urothelial carcinoma by down-regulating aurora A, HIF-1α and Bcl-2 both in vitro and in vivo. 丹参酮 IIA 通过下调极光 A、HIF-1α 和 Bcl-2 在体外和体内对膀胱尿路上皮癌的抗癌作用

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Ning Xiao, Yao Huang, Qi Tang, Hong Chen, Jin Hua Xiao, Chu Yang Huang, Xiangxi Yao, Hua Sheng Zhao

{"title":"Anticancer effects of tanshinone IIA in bladder urothelial carcinoma by down-regulating aurora A, HIF-1α and Bcl-2 both in vitro and in vivo.","authors":"Ning Xiao, Yao Huang, Qi Tang, Hong Chen, Jin Hua Xiao, Chu Yang Huang, Xiangxi Yao, Hua Sheng Zhao","doi":"","DOIUrl":"","url":null,"abstract":"The mechanisms of the anticancer effect of Tanshinone IIA (Tan IIA) on Bladder urothelial carcinoma (BUC) remain mostly unknown. In this study, BUC T24 cells were treated with Tan IIA at different concentrations and durations. The apoptosis, proliferation and invasion of T24 cells were evaluated using MTT assays, Annexin V-FITC Staining, Hoechst staining and Trans well assay. One group of T-24 cell xenograft mice was treated with Tan IIA, while the other group received normal saline for 25 days. Subsequently, the size of tumors as well as mRNA and protein expression of Aurora A, HIF-1α and Bcl-2 were measured both in vitro and in vivo. Tan IIA induced apoptosis, inhibited proliferation, suppressed invasion of T24 cells in a time- and dose-dependent manner in vitro and attenuated growth in vivo. The decreasing of mRNA and protein expression of Aurora A, HIF-1α and Bcl-2 in T-24 cells treated with Tan IIA were detected in a time- and dose-dependent manner both in vitro and in vivo. The pro-apoptotic, anti-proliferative and anti-invasive effects of Tan IIA on T-24 cells may be derived from inhibition of mRNA and protein expression of Aurora A, HIF-1α and Bcl-2. Tan IIA could potentially serve as a novel potential anti-cancer agent for BUC.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KLF6 aggravates myocardial fibrosis by promoting mitochondrial division. KLF6 通过促进线粒体分裂加重心肌纤维化。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-05-01

Tingting Zhang, Hongyao Ge, Qiuhang Song, Gaoshan Yang, Aiying Li

引用次数: 0