Pakistan journal of pharmaceutical sciences最新文献_第7页

Revealing analgesic and anxiolytic potentials of synthetic benzimidazole analogues: An in-vivo and in-silico study. 揭示合成苯并咪唑类似物的镇痛和抗焦虑潜力：体内和微观研究

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Shamim Akhtar, Sabahat Naeem, Nadia Asghar, Faisal Muhammad Khan, Moona Mehboob Khan, Arfa Akram, Imran Suheryani

{"title":"Revealing analgesic and anxiolytic potentials of synthetic benzimidazole analogues: An in-vivo and in-silico study.","authors":"Shamim Akhtar, Sabahat Naeem, Nadia Asghar, Faisal Muhammad Khan, Moona Mehboob Khan, Arfa Akram, Imran Suheryani","doi":"","DOIUrl":"","url":null,"abstract":"Certain drugs have potential to affect and alter individual's behavior. On the other hand, pain is a complex phenomenon with various treatment options; analgesic medicines are the primary source. Therefore, this study was based on examining some of the benzimidazole analogues for their analgesic as well as behavioral potential following Tail immersion test and Open field test respectively. In addition, molecular docking was performed to find the interaction of these compounds with the active site using AutoDock Vina which was further visualized through Discovery Studio Visualizer. It was seen that the cyano-methyl benzimidazole derivatives (CMB1-CMB3) showed relief in pain as compared to benzimidazole derivatives (BI1-BI3), CMB2 demonstrated highly potent analgesic effect. Likewise, all structures except BI1 displayed increase locomotion during open field test and can be offered as anxiolytic compounds. Almost all derivatives showed improve binding energies for the tested proteins where the high analgesic action of CMB2 might be correlated to its high binding affinity and interaction at µOR. It was also noticed that all structures except BI showed possible binding interaction with GABAA receptor and hence possessed anxiolytic like potential. Thus, this study offered benzimidazole analogues for further drug development of analgesic and anxiolytic like compounds.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation of Aloe vera based curcumin topical gel and its in-vitro evaluation. 芦荟姜黄素外用凝胶的配制及其体外评估

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Maira Shuja, Imtiaz Majeed, Tehseen Riaz, Muhammad Ahsan Waqar, Naila Tabassam, Tooba Mehboob, Abeeha Imtiaz

{"title":"Formulation of Aloe vera based curcumin topical gel and its in-vitro evaluation.","authors":"Maira Shuja, Imtiaz Majeed, Tehseen Riaz, Muhammad Ahsan Waqar, Naila Tabassam, Tooba Mehboob, Abeeha Imtiaz","doi":"","DOIUrl":"","url":null,"abstract":"Curcumin is a polyphenolic compound obtained from the rhizome of plant. Curcuma longa possesses antioxidant, anti-inflammatory and wound-healing properties. The current study was designed to formulate an Aloe vera-based curcumin topical gel. To enhance curcumin's solubility, it was first complexed with β-cyclodextrin, given its hydrophobic nature. While Carbopol, carboxy methyl cellulose and guar gum were used in various concentrations as gelling agents for preparation of the formulations. The effect of propylene glycol as a permeation enhancer was also observed. The prepared formulations were tested for different parameters such as physical appearance, spreadability, drug content, pH, viscosity and in-vitro permeation. All the formulations were found to be stable. All formulations consisting of propylene glycol showed permeation within the range of 80-90%. The maximum percentage of drug release was observed in the formulation containing 1% Carbopol 940 as the gelling agent which also exhibited good spreadability. In comparison to gels formulated with carboxymethyl cellulose and guar gum, Carbopol 940 gels appeared more translucent. Consequently, it was concluded that curcumin's permeation improved following its complexation with β-cyclodextrin. This complex when further used for the formation of an aloe vera based topical gel with 1% Carbopol 940 and 10% propylene glycol demonstrated maximum efficacy.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HuangJinShuangShen granules can improve immunity and enhance the ability of 5-fluorouracil to induce apoptosis of gastric cancer cells. 黄精双参颗粒能提高机体免疫力，增强5-氟尿嘧啶诱导胃癌细胞凋亡的能力。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Xuefei Xu, Chuqi Yang, Mengting Wu, Qingwen An, Tao Jiang, Guangji Zhang

{"title":"HuangJinShuangShen granules can improve immunity and enhance the ability of 5-fluorouracil to induce apoptosis of gastric cancer cells.","authors":"Xuefei Xu, Chuqi Yang, Mengting Wu, Qingwen An, Tao Jiang, Guangji Zhang","doi":"","DOIUrl":"","url":null,"abstract":"In recent years, Chinese herbal compounds have gained significant prominence in the treatment of gastric cancer. The goal of this study was to investigate the antitumor effect of HuangJinShuangShen granules (HJSS) combined with 5-fluorouracil on MFC gastric cancer mice. In this study, the MFC model with gastric cancer was successfully established. After continuous administration for 14 d, the body weight, tumor volume and weight and spleen mass of mice in each group were recorded. The levels of IFN-γ and TGF-β1 in serum were detected by ELISA. The expression of apoptosis proteins in tumor tissues was detected by Western blotting. Compared with the model group and the 5-FU group, the combined drug group can significantly inhibit tumor growth, reduce tumor volume, promote tumor cell necrosis and increase spleen index in mice. At the same time, the combined treatment group significantly increased IFN-γ level and BAX protein expression, decreased TGF-β1 level and decreased Bcl2, Caspase-9 and Cleaved Caspase-3 protein expressions. These findings provide evidence that HJSS can augment the suppressive impact of 5-FU on tumor growth in gastric cancer mice, potentially through the induction of tumor cell apoptosis and the restoration of immune function.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of Lamivudinyl palmitate nanoemulsome for liver-targeting delivery. 制备用于肝脏靶向递送的拉米夫定棕榈酸酯纳米乳剂。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Chang Liu, Tao Xu, Xiaoyu Sui, Jing Wang, Cuiyan Han, Xiaoxing Ma, Jiayi Qian, Wenquan Zhu

{"title":"Preparation of Lamivudinyl palmitate nanoemulsome for liver-targeting delivery.","authors":"Chang Liu, Tao Xu, Xiaoyu Sui, Jing Wang, Cuiyan Han, Xiaoxing Ma, Jiayi Qian, Wenquan Zhu","doi":"","DOIUrl":"","url":null,"abstract":"The water solubility and side effects of lamivudine limit its application for the treatment of viral hepatitis type B and human immunodeficiency virus. In order to increase the solubility of LA and improve the in vivo membrane permeability of the drug, LA was modified with hexadecane acid to prepare the prodrug lamivudine palmitic acid (LAP) and loaded into nanoemulsome (NES). LAP-NES was prepared by the thin film dispersion method. The LAP-NES showed the sustained release performance up to 72h in pH 7.4 PBS. Moreover, the pharmacokinetics of LAP-NES after tail vein injection in rats and the biodistribution characteristics were evaluated. The tmax of LAP-NES was 2.5h. The t1/2, clearance rate and average retention time of LAP-NES obviously prolonged compared with free LAP. The tissue biodistribution behavior of NES in vivo showed the good targeting in the liver and spleen, with the maximum at 4h and then the fluorescence slowly decreased until 72h. LAP-NES could significantly delay the release of LA in vivo, effectively prolong the elimination time and had obvious liver-targeting ability. In summary, LAP-NES shows great potential for liver-targeting delivery to increase the therapeutic effect and decrease the side effects of LA.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of neuropharmacological consequences induced by dexamethasone administration in rats model of neurodegenerative diseases. 评估在神经退行性疾病大鼠模型中使用地塞米松引起的神经药理学后果。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Muhammad Farhan, Samia Khan, Ayesha Ahmed Soomro, Shoaib Ahmed, Anila Bibi, Syeda Rabab Zehra, Maria Arshad, Fatima Riaz, Sadia Rehman, Saher Asif

{"title":"Assessment of neuropharmacological consequences induced by dexamethasone administration in rats model of neurodegenerative diseases.","authors":"Muhammad Farhan, Samia Khan, Ayesha Ahmed Soomro, Shoaib Ahmed, Anila Bibi, Syeda Rabab Zehra, Maria Arshad, Fatima Riaz, Sadia Rehman, Saher Asif","doi":"","DOIUrl":"","url":null,"abstract":"Stress is a well-known and frequently used term among present generation. It has been referred to the response of body to any challenge for a change. It is a natural process and our body is designed to cope with it. However, if stress becomes chronic, it can lead to mental health problems. Stress due to the prolonged administration of glucocorticoid is enabled to produce impressive alterations in rats model shoeing depressive like behavior. In this investigation; purpose was to study the impact of episodic treatment of dexamethasone with respect to behavioral changes in rats. It was hypothesized that repeated administration of dexamethasone could increase stress and thus, psychological stress leading to mood disorders and behavior deficits in rats. Rats were injected daily with DEX (10 mg/ml/kg, orally) and the different behavioral models of the animals were assessed. DEX-treated rats exhibited depressive behavior like greater time to start mobility in a novel environment and elevated anxiety-like behavior in elevated plus maze. However, time spent in light compartment was shorter with repeated administration of DEX. From results it is demonstrated that the administration of DXM for weeks induced stress and consequently, induced a depression-like behaviors in rats models.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moringa oleifera-mediated iron oxide nanoparticles, characterization and their anti-proliferative potential on MDA-MB 231 human breast cancer cells. 油杉介导的氧化铁纳米粒子、特性及其对 MDA-MB 231 人类乳腺癌细胞的抗增殖潜力。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Huzaifa Umar, Maryam Rabiu Aliyu

{"title":"Moringa oleifera-mediated iron oxide nanoparticles, characterization and their anti-proliferative potential on MDA-MB 231 human breast cancer cells.","authors":"Huzaifa Umar, Maryam Rabiu Aliyu","doi":"","DOIUrl":"","url":null,"abstract":"Iron oxide nanoparticles (Fe2O3 NPs) stabilized with Moringa oleifera (M.O.) were successfully synthesized. The study aimed to explore the cytotoxic, anti-proliferative and anti-microbial potential of Fe2O3 NPs through various assays, including trypan blue, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), wound healing and disc diffusion assay. The Moringa oleifera-mediated Fe2O3 NPs (M O Fe2O3 NPs) underwent analysis using various techniques, including Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-vis spectroscopy (UV-vis), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). FTIR, XRD and SEM-EDX results confirmed the successful synthesis of Fe2O3 NPs. The UV-Vis spectra analysis indicated an absorption peak at 314 nm, ensuring both the successful synthesis and remarkable stability of the nanoparticles. The nanoparticles displayed a uniform spherical morphology and contained Fe, O, confirming the formation of M O Fe2O3 NPs. Cytotoxic and anti-proliferative potential on MDA-MB 231 human breast cancer cells were observed with various concentrations of M O Fe2O3 NPs and the cytotoxicity result revealed an IC50 of 69.7μg/mL. In conclusion, stable Fe2O3 NPs were synthesized using a methanolic extract of M.O. and they demonstrated antimicrobial activity against both gram-negative and gram-positive bacteria. The nanoparticles exhibited cytotoxicity and anti-proliferative activity on MDA-MB 231 human breast cancer cell lines.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological activities of crude leaf extract and fractions of Scutellaria sibthorpii (Benth.) Halácsy: An endemic plant of North-Cyprus. Scutellaria sibthorpii (Benth.) Halácsy 的粗叶提取物和馏分的生物活性：北塞浦路斯的一种特有植物。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Cagin Korkmazer, Emmanuel Mshelia Halilu

{"title":"Biological activities of crude leaf extract and fractions of Scutellaria sibthorpii (Benth.) Halácsy: An endemic plant of North-Cyprus.","authors":"Cagin Korkmazer, Emmanuel Mshelia Halilu","doi":"","DOIUrl":"","url":null,"abstract":"Scutellaria sibthorpii is used in treatment of bacterial infections, pains and inflammations. The leaf was extracted by maceration and then partitioned with hexane, ethyl acetate and n-butanol. The extract was screened for phytochemicals. The antioxidant activity was assayed using DPPH, H2O2 and β-carotene. The total phenolic and flavonoid contents were estimated using Folin-Ciocalteu's and AlCl3. The crude extract/fractions were tested on E. coli, S. typhi, B. subtilis and S. aureus using agar disk diffusion. Lorke's method was used to determine the LD50. The analgesic activity was determined using ethanoic acid-induced writhing, hotplate and formalin-induced nociception. Anti-inflammatory activity was investigated by means of formalin-induced acute inflammation. The antipyretic activity was studied using Brewer's yeast. The ethyl acetate fraction (EtFSs) had IC50 of 0.4352mg/mL in the H2O2 and IC50 of 0.00014mg/mL. The MIC of EtFSs against S. aureus was 1.25mg/mL and 2.5mg/mL on S. typhi. LD50 of the CrESs/fractions were greater than 5000mg/Kg. The analgesic and anti-inflammatory activities were higher in EtFSs when compared to piroxicam. The fractions decrease the rectal temperature of the rats in the same way as paracetamol at 200mg/Kg. This research has for the first time validated the used of Scutellaria sibthorpii traditionally as analgesic, anti-inflammatory and antipyretic.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of bushen tongluo decoction on the ERK/c-Fos signal transduction pathway in rats with bone cancer pain. 蒲参通络汤对骨癌痛大鼠ERK/c-Fos信号转导通路的影响

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Xinchao Jiang, Yi Song, Mei Fang, Biao Zhang, Yinyin Guo, Zhijun Fang, Jianxue Qian

{"title":"Effect of bushen tongluo decoction on the ERK/c-Fos signal transduction pathway in rats with bone cancer pain.","authors":"Xinchao Jiang, Yi Song, Mei Fang, Biao Zhang, Yinyin Guo, Zhijun Fang, Jianxue Qian","doi":"","DOIUrl":"","url":null,"abstract":"Bone cancer pain could lead to pain sensitization. Traditional Chinese Medicine could relieve bone cancer pain (BCP). This study aimed to investigate the analgesic effect of Bushen Tongluo decoction on rats with BCP and its impact on ERK/c-fos pathway in spinal dorsal horn. Cancer cells were injected to induce bone cancer pain rats. Inflammatory factors in serum were determined using enzyme-linked immunosorbent. ERK/c-Fos in the spinal dorsal horn were detected using western blotting and RT-qPCR. Thermal hyperalgesia and mechanical allodynia were observed in BCP rats. The ERK/c-Fos pathway activation was observed in the spinal dorsal horn and the expression of inflammatory cytokines increased in the serum. Bushen Tongluo decoction alleviated inflammatory cytokines and reduced the ERK/c-Fos pathway. We provided evidence that Bushen Tongluo decoction exhibits a potential and beneficial effect on inflammatory cytokines, effectively alleviating allodynia and hyperalgesia in rats with bone cancer. This effect may be attributed to down-regulation of the ERK/c-Fos pathway in spinal dorsal horn and serum inflammatory cytokines.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, characterization and in-vitro cytotoxic potential of sitagliptin phosphate nanoparticles against advanced breast cancer cell line - MCF-7. 磷酸西他列汀纳米颗粒的合成、表征和体外细胞毒性潜力，用于抗晚期乳腺癌细胞株 MCF-7。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Muhammad Abdullah, Azra Rafiq, Nariman Shahid, Muhammad Nasir Kalam, Yusra Munir, Muhammad Daoud Butt, Hamid Saeed

{"title":"Synthesis, characterization and in-vitro cytotoxic potential of sitagliptin phosphate nanoparticles against advanced breast cancer cell line - MCF-7.","authors":"Muhammad Abdullah, Azra Rafiq, Nariman Shahid, Muhammad Nasir Kalam, Yusra Munir, Muhammad Daoud Butt, Hamid Saeed","doi":"","DOIUrl":"","url":null,"abstract":"Pharmaceutical substance sitagliptin has long been used to treat diabetes. However, subsequent researches have shown that sitagliptin has additional therapeutic effects. Anti-inflammatory effects are observed. Combining sitagliptin with biodegradable polymers like nanoparticles for chemotherapy may be effective. This method enhances therapeutic agent pharmacokinetics. This study tests sitagliptin (SIT) chitosan base nanoparticles against MCF-7 cancer cell lines for anti-cancer effects. Sitagliptin chitosan-based nanoparticles are tested for their ability to suppress MCF-7 cancer cell proliferation. Ionic gelation, a typical nanoparticle manufacturing method, was used. A detailed examination of the nanoparticles followed, using particle-size measurement, FTIR and SEM. Entrapment efficiency, drug-loading, and in-vitro drug release were assessed. Loaded with chitosan and sitagliptin, the nanoparticles averaged 500nm and 534nm in diameter. Sitagliptin has little effect on particle size. Chitosan-based Sitagliptin nanoparticles grew slightly, suggesting Sitagliptin is present. SIT-SC-NPs had 32% encapsulation efficiency and 30% drug content due to their high polymer-to-drug ratio. SEM analysis showed that both drug-free and sitagliptin-loaded nanoparticles are spherical, as shown by the different bands in the photos. The SIT-CS-NPs had a 120-hour release efficiency of up to 80%. This suggests that these nanoparticles could cure hepatocellular carcinoma, specifically MCF-7 cell lines.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of vitamin D supplementation in augmenting IFN-γ production in response to mycobacterium tuberculosis Infection: A randomized controlled trial. 补充维生素 D 在结核分枝杆菌感染时增强 IFN-γ 生成的作用：随机对照试验。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Muhammad Imran Hussain, Ramisha Ibtisam, Tayyaba Fatima, Huba Khalid, Ayesha Aziz, - Khansa, Adan Sitara, Anam Shahzad, Aymen Jabeen

{"title":"The role of vitamin D supplementation in augmenting IFN-γ production in response to mycobacterium tuberculosis Infection: A randomized controlled trial.","authors":"Muhammad Imran Hussain, Ramisha Ibtisam, Tayyaba Fatima, Huba Khalid, Ayesha Aziz, - Khansa, Adan Sitara, Anam Shahzad, Aymen Jabeen","doi":"","DOIUrl":"","url":null,"abstract":"Vitamin D supports the immune system fight TB by inhibiting Interferon-gamma (IFN-γ) and lowering host inflammation. The purpose of the research was to see if giving the vitamin D supplements to TB patients affected their prognosis. A randomized placebo control study of 200 TB patients was performed among which 106 received 400,000 IU of injectable vitamin D3 and 94 received placebo for 2 doses. Assessment was carried out at the end of every month for 3 months. IFN-γ responses to whole blood stimulation generated by the Mycobacterium tuberculosis sonicate (MTBs) antigen and early secreted and T cell activated 6 kDa (ESAT6) were assessed at 0 and 12 weeks. The statistical analysis used descriptive statistics (mean and standard deviation), Friedman's test and Fisher's test. The vitamin D group gained significantly more weight (+3.90 pounds) and had less persistent lung disease on imaging (1.33 zones vs. 1.84 zones). They also had a 50% decrease in cavity size. Additionally, patients with low baseline serum concentrations of 25-(OH)D had a significant increase in MTB-induced IFN-γ production after taking vitamin D supplements. Vitamin D administration in large amounts can hasten the recovery of TB patients. The findings point is a therapeutically useful activity of Vitamin D's in the management for tuberculosis.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0