Pakistan journal of pharmaceutical sciences最新文献

Evaluating the efficacy and safety of empagliflozin addition to insulin and oral antidiabetic medication (OAD) regimen in poorly controlled type 2 diabetes and obese patients. 评估在胰岛素和口服抗糖尿病药物（OAD）治疗方案中添加empagliflozin对控制不佳的2型糖尿病和肥胖患者的疗效和安全性。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Hina Sharif, Sana Sharif Sheikh, Ambreen Salman, Zahida Jawed, Ishrat Karim, Tehseena Sohail, Nadia Mohsin

{"title":"Evaluating the efficacy and safety of empagliflozin addition to insulin and oral antidiabetic medication (OAD) regimen in poorly controlled type 2 diabetes and obese patients.","authors":"Hina Sharif, Sana Sharif Sheikh, Ambreen Salman, Zahida Jawed, Ishrat Karim, Tehseena Sohail, Nadia Mohsin","doi":"","DOIUrl":"","url":null,"abstract":"Insulin resistance complicates diabetes care. Its effectiveness and tolerability as an addition to metformin, DPP4 inhibitor and insulin treatment in type 2 diabetic patients will be examined in this research. Participants with type 2 diabetes from poor socio-economic backgrounds had HbA1c values ≥8.5% when using Insulin+Metformin+DPP-4 inhibitors. They received 10mg Empagliflozin daily for 12 weeks (n=143). The main outcome was change in HbA1c at 12th week from baseline. Secondary outcomes were baseline weight and week 12 FPG. Adjusted mean (SE) HbA1c increases at week 12 were: Mean ± SD 10.38 (6.8-17.0) vs. Mean±SD 9.05±1.77 (5.60-16.0) with empagliflozin 10mg. When added to the regimen, empagliflozin significantly reduced FPG, systolic and diastolic blood pressure. The mean (SE) BMI increases from baseline were 31.28±5.89 (16.0-66.0) and 29.73±5.47 (3.0-46.0) with 10mg empagliflozin. Two individuals experienced urinary tract infections as AEs, but no genital infections. Adding empagliflozin 10mg daily to metformin+DPP4 inhibitor+insulin improved glycemic control, body weight and blood pressure for 12 weeks. The intervention was well-tolerated, highlighting empagliflozin's therapeutic potential.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental study on the antifatigue effect of icariin. 关于冰片苷抗疲劳作用的实验研究。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Mosi Chen, Qi Li, Chenglan Su, Yuping Chen, Li Dai, Genlin Chen

{"title":"Experimental study on the antifatigue effect of icariin.","authors":"Mosi Chen, Qi Li, Chenglan Su, Yuping Chen, Li Dai, Genlin Chen","doi":"","DOIUrl":"","url":null,"abstract":"Fatigue is a serious disturbance to human health, especially in people who have a severe disease such as cancer, or have been infected with COVID-19. Our research objective is to evaluate the anti-fatigue effect and mechanism of icariin through a mouse experimental model. Mice were treated with icariin for 30 days and anti-fatigue effects were evaluated by the weight-bearing swimming test, serum urea nitrogen test, lactic acid accumulation and clearance test in blood and the amount of liver glycogen. The protein expression levels of adenosine monophosphate-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC1-α) in the skeletal muscle of mice in each group were measured by western blotting. Results showed that icariin prolonged the weight-bearing swimming time of animals, reduced the serum urea nitrogen level after exercise, decreased the blood lactic acid concentration after exercise and increased the liver glycogen content observably. Compared to that in the control group, icariin upregulated AMPK and PGC1-α expression in skeletal muscle. Icariin can improve fatigue resistance in mice and its mechanism may be through improving the AMPK/PGC-1α pathway in skeletal muscle to enhance energy synthesis, decreasing the accumulation of metabolites and slowing glycogen consumption and decomposition.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile synthesis of silver and gold nanoparticles using mangrove (Avicennia marina) leaves extract and its cytotoxicity and larvicidal activity. 利用红树林（Avicennia marina）叶提取物轻松合成银和金纳米粒子及其细胞毒性和杀幼虫剂活性。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Hamed A Gramah, Zubair Ahmad, Essam H Ibrahim

{"title":"Facile synthesis of silver and gold nanoparticles using mangrove (Avicennia marina) leaves extract and its cytotoxicity and larvicidal activity.","authors":"Hamed A Gramah, Zubair Ahmad, Essam H Ibrahim","doi":"","DOIUrl":"","url":null,"abstract":"The field of bio-fabricated noble metallic nanoparticles (NPs) has gained significant attention in applied research due to their eco-friendly and biocompatible nature. This study focuses on employing a green synthesis method to produce silver and gold nanoparticles (bio-fabricated) using a Mangrove plant extract and assessing their insecticidal and growth-inhibitory effects for environmentally friendly pest control. The resulting NPs underwent comprehensive characterization through various spectroscopy techniques. The morphology of both silver and gold mediated nanoparticles of Avicennia marina leaf extract displayed a spherical shape, with average sizes measuring around 70-80 nm and 95-100 nm, respectively. Regarding cytotoxicity, the inhibitory effects of silver nanoparticles were less than that observed by the extract alone while gold nanoparticles showed stronger cell growth inhibitory effects on splenic cells. The hepatic toxicity of silver and gold nanoparticles showed significant toxic effects as compared to A. marina extract alone. Notably, as prepared silver nanoparticles exhibited substantial larvicidal toxicity as compared to gold nanoparticles, when tested against fourth instar Culex pipiens larvae. These biocompatible silver and gold nanoparticles prepared from A. marina leaf extract hold promise for future applications as larvicides to effectively control mosquito species.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory effects of steroidal alkaloids of Solanum lyratum Thunb. Solanum lyratum Thunb.甾体生物碱的抗炎作用

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Lijie Yang, Yiming Yang, Qi Wang, Hongtian Fei, Rui Fei, Yajuan Xu, Chunmei Zhang

引用次数: 0

Antioxidant potentials of extracts from different parts of Clinacanthus nutans (Burm. f.) Lindau. Clinacanthus nutans (Burm. f.) Lindau 不同部位提取物的抗氧化潜力。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Khanh Duy Dang, Chi Hoang Minh Nguyen, Thao Ngoc Nha Nguyen, Dai Thi Trang Nguyen

{"title":"Antioxidant potentials of extracts from different parts of Clinacanthus nutans (Burm. f.) Lindau.","authors":"Khanh Duy Dang, Chi Hoang Minh Nguyen, Thao Ngoc Nha Nguyen, Dai Thi Trang Nguyen","doi":"","DOIUrl":"","url":null,"abstract":"The research aimed to explore the antioxidant potential of extracts from different parts of Clinacanthus nutans growing in Vietnam, a member of the Acanthaceae family. The plant's roots, stem and leaves were extracted using 96% ethanol. The antioxidant actions of these extracts were evaluated by DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay on thin-layer plates and 96 well plates. The extract with the most potent activity was applied for distribution extraction with solvents with different polarities, including dichloromethane, ethyl acetate and water. Dry column vacuum chromatography was utilized to obtain the most antioxidant-potent extract fractions. The stem extract had the lowest IC50 value of 6.85µg/mL, showing the most potent antioxidant activity. The ethyl acetate fraction from the stem extract expressed the lowest IC50 value of 9.67µg/mL. Meanwhile, fraction 5, separated from the ethyl acetate fraction of the stem extract, had the lowest IC50 value of 9.89µg/mL. In conclusion, the extracts from different parts of Clinacanthus nutans all expressed antioxidant action at different levels, in which the stem extract, the ethyl acetate fraction and fraction 5 from the ethyl acetate fraction displayed the most effective actions. These findings highlight the promising potential of Clinacanthus nutans in treating oxidative stress-associated diseases, inspiring further research and exploration in this area.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Zingiber officinale bioactive compounds for inhibitory effects on Streptococcus pneumoniae capsular polysaccharide biosynthesis proteins: In silico study. 探索姜科植物生物活性化合物对肺炎链球菌囊多糖生物合成蛋白的抑制作用：硅学研究。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Muhammad Bilal Azmi, Muhammad Yahya Noori, Syed Danish Haseen Ahmed, Bader Saud Alotaibi, Sadaf Naeem, Mohsin Kazi, Muhammad Islam, Abdul Wadood

{"title":"Exploring Zingiber officinale bioactive compounds for inhibitory effects on Streptococcus pneumoniae capsular polysaccharide biosynthesis proteins: In silico study.","authors":"Muhammad Bilal Azmi, Muhammad Yahya Noori, Syed Danish Haseen Ahmed, Bader Saud Alotaibi, Sadaf Naeem, Mohsin Kazi, Muhammad Islam, Abdul Wadood","doi":"","DOIUrl":"","url":null,"abstract":"The capsule is a major virulence factor for Streptococcus pneumoniae which causes global morbidity and mortality. It is already known that there are few conserved genes in the capsular biosynthesis pathway, which are common among all known serotypes, called CpsA, CpsB, CpsC and CpsD. Inhibiting capsular synthesis can render S. pneumoniae defenseless and vulnerable to phagocytosis. The Inhibitory potential of active Zingiber officinale compounds was investigated against the 3D (3-dimensional) structural products of Cps genes using in silico techniques. A 3D compound repository was created and screened for drug-likeness and the qualified compounds were used for molecular docking and dynamic simulation-based experiments using gallic acid for outcome comparison. Cavity-based docking revealed five different cavities in the CpsA, CpsB and CpsD proteins, with gallic acid and selected compounds of Zingiber in a binding affinity range of -6.8 to -8.8 kcal/mol. Gingerenone A, gingerenone B, isogingerenone B and gingerenone C showed the highest binding affinities for CpsA, CpsB and CpsD, respectively. Through the Molegro Virtual Docker re-docking strategy, the highest binding energies (-126.5 kcal/mol) were computed for CpsB with gingerenone A and CpsD with gingerenone B. These findings suggest that gingerenone A, B and C are potential inhibitors of S. pneumoniae-conserved capsule-synthesizing proteins.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical composition of cedar tar of the atlas and it's in vitro antifungal activity against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. 图谱中杉木焦油的植物化学成分及其对红毛癣菌、门冬癣菌和犬小孢子菌的体外抗真菌活性。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Sanae Masmoudi, Abdelmjid Aiboud, Laila Chaoui, Fatima Zahra Milouk, Ahmed Moussaif, Aboubaker El Hessni, Abdelhalim Mesfioui

{"title":"Phytochemical composition of cedar tar of the atlas and it's in vitro antifungal activity against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis.","authors":"Sanae Masmoudi, Abdelmjid Aiboud, Laila Chaoui, Fatima Zahra Milouk, Ahmed Moussaif, Aboubaker El Hessni, Abdelhalim Mesfioui","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study was to identify the major compounds present in Cedar tar obtained by distillation of Cedrus atlantica wood from the Taza forest (Morocco) and to evaluate its antidermatophytic activity in vitro against the three strains of dermatophytes most widespread in Morocco, considered the main prevailing causes of fungal infections of the skin, hair and nails. GC/MS analysis revealed that cedar tar is composed mainly of hydrocarbon sesquiterpenes and oxygenated sesquiterpenes, with nine major compounds identified, including α-Cedrene, β-Cadinene, γ-Cadinene, β-Himachelene, α-Turmerone, β-Turmerone, Ar-tumerone, α-Atlantone and Himachalol. The evaluation of antifungal activity was carried out by the micro dilution technique. The MIC values found were 100µg/mL, 2µg/mL and 0.1µg/mL on Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis strains respectively. The observed strong antifungal activity of cedar tar is attributed to the prevalence of oxygenated and hydrocarbon sesquiterpenes, known for their established antidermatophytic properties. This study highlights the potential of the Atlas Cedar tar as an effective antifungal agent for the treatment of superficial mycoses, particularly dermatophytoses.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and in vitro/in vivo evaluation of a pantoprazole sodium drug-resin liquid delayed release suspension. 泮托拉唑钠药物-树脂液体缓释混悬液的制备及体内外评估

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Xinyi Tang, Xianghuan Meng, Ke Li, Zhannuan Yin, Haibing He, Hongfei Liu

{"title":"Preparation and in vitro/in vivo evaluation of a pantoprazole sodium drug-resin liquid delayed release suspension.","authors":"Xinyi Tang, Xianghuan Meng, Ke Li, Zhannuan Yin, Haibing He, Hongfei Liu","doi":"","DOIUrl":"","url":null,"abstract":"A drug-resin liquid delayed-release suspension of pantoprazole sodium (PAZ-Na) was prepared to improve the effectiveness, convenience and safety of peptic ulcer treatment in children, the elderly and patients with dysphagia. Pantoprazole sodium drug-resin complexes (PAZ-Na-DRC) were prepared using the bath method. The fluidized bed coating method is used to coat it and then add excipients to make a dry suspension prepared before use. The parameters of the in vitro release experimental conditions were optimized and the drug release curve showed delayed release. Rats were given commercial PAZ-Na enteric-coated pellet capsules and the PAZ-Na delayed release suspension via intragastric administration. The results showed that the Tmax of the PAZ-Na delayed release suspension was increased from 2h to 4h compared with the PAZ-Na enteric-coated pellet capsules. Similarly, the Cmax was reduced from 6.162μg/mL to 3.244μg/mL with the concentration-time curve is very gentle compared with the commercial drug capsules. After oral administration, the relative bioavailability of PAZ-Na delayed release suspension (AUC0-24 of 19.578 μg•h•mL-1) compared with the commercial drug (AUC0-24 of 17.388 μg•h•mL-1) was 112.67%. The findings showed that the PAZ-Na delayed release suspension for oral administration was successfully formulated with highly improved pharmacokinetic indices.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the combination of black rice bran ethanol extract and glimepiride in reducing blood glucose and protecting kidney, liver and pancreatic cells. 评估黑米糠乙醇提取物和格列美脲的组合在降低血糖和保护肾脏、肝脏和胰腺细胞方面的作用。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Diski Wahyu Wijianto, Arifah Sri Wahyuni, Rizkiananda Wardani, Ambar Yunita Nugraheni, Fazleen Izzany Abu Bakar

引用次数: 0

Quantification of mangiferin in streptozotocin-induced diabetic rat plasma using UPLC-MS/MS: Pharmacokinetic assessment of Gentiana rhodantha extract after oral administration. 利用 UPLC-MS/MS 对链脲佐菌素诱导的糖尿病大鼠血浆中的芒果苷进行定量：对口服龙胆草提取物后的药代动力学进行评估。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2024-03-01

Ying Zhao, Jian- Wu Li

引用次数: 0