Pakistan journal of pharmaceutical sciences最新文献

{"title":"miR-152-3p inhibits triple-negative breast cancer cell progression by targeting signal transducer and activator of transcription 3, nuclear factor kappa B subunit p65 and adenylate cyclase 6.","authors":"Yan Cheng, Yingyao Quan","doi":"10.36721/PJPS.2026.39.7.202.1","DOIUrl":"10.36721/PJPS.2026.39.7.202.1","url":null,"abstract":"<p><strong>Background: </strong>Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype and currently lacks defined therapeutic targets. Although miR-152-3p functions as a tumor suppressor in various cancers, its specific mechanism and regulatory network in TNBC remain poorly understood.</p><p><strong>Objectives: </strong>To investigate the expression and tumor-suppressive function of miR-152-3p in TNBC cells and to elucidate its mechanism of targeting STAT3, RELA and ADCY6.</p><p><strong>Methods: </strong>miR-152-3p expression was compared between MDA-MB-231 and MCF-10A cells using qRT-PCR. MDA-MB-231 cells were transfected with miR-152-3p mimics or inhibitors and cell proliferation, apoptosis and invasion were assessed by MTT assay, flow cytometry and Transwell assay, respectively. Direct target interactions were validated by a dual-luciferase reporter assay, and protein levels of STAT3, RELA, and ADCY6 were examined by Western blot. Key findings were further validated in Hs 578T cells.</p><p><strong>Results: </strong>miR-152-3p expression was significantly downregulated in TNBC cells. Overexpression of miR-152-3p markedly inhibited proliferation and invasion while promoting apoptosis in MDA-MB-231 cells. Dual-luciferase reporter assays confirmed that miR-152-3p directly binds to the 3'untranslated regions of STAT3, RELA and ADCY6. Overexpression of miR-152-3p significantly reduced STAT3 and RELA protein levels while upregulating ADCY6 expression. Rescue experiments demonstrated that restoration of STAT3 expression partially reversed the tumor-suppressive effects of miR-152-3p. These findings were recapitulated in Hs 578T cells, suggesting generalizability across TNBC subtypes.</p><p><strong>Conclusion: </strong>miR-152-3p suppresses TNBC progression by downregulating STAT3/RELA and upregulating ADCY6, thereby activating cAMP signaling. These findings provide a foundation for further investigation into the potential of miR-152-3p as a multi-target therapeutic strategy for TNBC.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"2138-2152"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atorvastatin and nitroglycerin ointment in digit replantation: Associations with vascular crisis and circulating biomarkers.","authors":"Weihua Zhang","doi":"10.36721/PJPS.2026.39.7.186.1","DOIUrl":"10.36721/PJPS.2026.39.7.186.1","url":null,"abstract":"<p><strong>Background: </strong>Vascular crisis is a major threat to digit replantation success, leading to substantial failure rates and compromised functional recovery.</p><p><strong>Objectives: </strong>To assess the association of combined atorvastatin and nitroglycerin ointment with vascular crisis, circulating biomarkers and clinical outcomes in patients undergoing unilateral digit replantation.</p><p><strong>Methods: </strong>184 patients were prospectively allocated to combination (atorvastatin + nitroglycerin) or control (nitroglycerin alone) groups. Vascular crisis incidence, serum biomarkers (TNF-α, IL-6, CRP, NO, eNOS, ET-1), microcirculation indices and 30-day digit survival were systematically monitored.</p><p><strong>Results: </strong>Combination therapy decreased vascular crisis incidence (6.52% vs. 16.30%, P=0.037) and improved 30-day digit survival (90.22% vs. 79.35%, P=0.040). Postoperatively, the combination group exhibited lower levels of TNF-α, IL-6, CRP and ET-1, along with higher NO/eNOS levels and improved microcirculation, with all differences statistically significant after Bonferroni correction (P<0.05).</p><p><strong>Conclusion: </strong>Atorvastatin combined with nitroglycerin ointment correlates with reduced vascular crisis and favorable 30-day outcomes, accompanied by beneficial shifts in biomarkers and microcirculation that may underpin the therapeutic effects.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"1957-1963"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ipratropium bromide nebulization plus SOM: Protective effects in severe pneumonia with respiratory failure.","authors":"Xiao Fang, Fangfang Zhang, Tingting Wu","doi":"10.36721/PJPS.2026.39.7.197.1","DOIUrl":"10.36721/PJPS.2026.39.7.197.1","url":null,"abstract":"<p><strong>Background: </strong>The additional pharmacological effects of ipratropium bromide, a short-acting anticholinergic agent, remain controversial within the context of standardized stress-oriented management (SOM).</p><p><strong>Objectives: </strong>To evaluate the effects of SOM combined with ipratropium bromide nebulization therapy on oxygenation status, respiratory mechanics, inflammatory stress responses and clinical outcomes in patients with severe pneumonia (SP) complicated with respiratory failure (RF) (SP+RF).</p><p><strong>Methods: </strong>This study retrospectively analyzed 168 patients with SP and RF admitted between March 2024 and August 2025 were enrolled and divided into an observation group (n = 81) and a control group (n = 87) based on whether they received ipratropium bromide nebulization therapy. Primary outcome measures included: oxygenation status, respiratory mechanics parameters, incidence of multiple organ dysfunction syndrome (MODS) and organ function indicators, inflammatory and stress markers, and clinical outcomes.</p><p><strong>Results: </strong>Regarding oxygenation status, the observation group demonstrated superior PaO₂/FiO₂, SpO₂ and blood lactate results compared to the control group at 24 hours post-treatment (T1) and 48 hours post-treatment (T2) (p<0.05). Respiratory mechanics parameters showed that airway resistance (Raw), peak airway pressure (Ppeak) and plateau pressure (Pplat) were lower in the observation group than in the control group at T1 (p<0.05); static lung compliance (Cst) showed more pronounced improvement at T2. Additionally, the incidence of MODS was lower in the observation group (p<0.05). Regarding inflammatory markers, the observation group exhibited lower levels of CRP and cortisol (p<0.05). Clinical outcomes demonstrated shorter DMV and length of hospital stay in the observation group (p<0.05), but no statistically significant difference was observed between the two groups in 28d-M (p>0.05).</p><p><strong>Conclusion: </strong>In patients with SP+RF, SOM plus ipratropium bromide nebulization significantly improves oxygenation status, respiratory mechanics, and inflammatory stress responses, shortens the duration of mechanical ventilation and the length of hospital stay, and reduces the risk of MODS.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"2089-2096"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced external counterpulsation (EECP) augmentation of amlodipine in elderly patients with isolated systolic hypertension (ISH) and wide pulse pressure: A comparative study.","authors":"Meili Ding, Jun Zhang, Zuliang Li, Jianqiao Yang","doi":"10.36721/PJPS.2026.39.7.203.1","DOIUrl":"10.36721/PJPS.2026.39.7.203.1","url":null,"abstract":"<p><strong>Background: </strong>Geriatric patients with isolated systolic hypertension (ISH) and wide pulse pressure (PP) were treated using enhanced external counter pulsation (EECP) combined with amlodipine versus amlodipine alone.</p><p><strong>Objectives: </strong>The efficacy of both treatment modalities was assessed based on hemodynamic parameters.</p><p><strong>Methods: </strong>Retrospectively included 132 elderly patients with ISH and wide PP in our hospital (Mar 2022-Jun 2024). After exclusion, 120 cases were analyzed and divided into the amlodipine group and the combined group. Primary indicators include endothelin-1 (ET-1), nitric oxide (NO), systolic blood pressure (SBP), PP, systemic vascular resistance (SVR), coronary flow reserve (CFR), flow-mediated vasodilatation (FMD); secondary measures include mean arterial pressure (MAP), wall shear stress (WSS) and adverse reaction incidence.</p><p><strong>Results: </strong>After 4 courses of treatment, patients in the combined group had significantly lower rates of ET-1, SBP, PP, MAP, SVR and the incidence of adverse reactions (all P<0.05); NO, FMD, WSS and CFR were higher (all P<0.05) than in the amlodipine group.</p><p><strong>Conclusion: </strong>EECP plus amlodipine has advantages over amlodipine monotherapy in treating elderly patients with ISH and wide PP, which provides a scientific basis for optimizing clinical treatment.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"2153-2164"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of curcumin on the signal transducer and activator of transcription 3 signaling pathway and bile acid enterohepatic circulation in liver cancer mice model.","authors":"Na Hu, Niu Yan, Tingting Zhao, Langui Hu","doi":"10.36721/PJPS.2026.39.7.207.1","DOIUrl":"10.36721/PJPS.2026.39.7.207.1","url":null,"abstract":"<p><strong>Background: </strong>Liver cancer has a high incidence and poor prognosis. Curcumin is a natural compound with anti-tumor activity, but its effects on the STAT3 signaling pathway and the bile acid enterohepatic circulation in liver cancer models remain unclear.</p><p><strong>Objectives: </strong>To investigate the effects of curcumin on the STAT3 signaling pathway and bile acid enterohepatic circulation in a mouse model of liver cancer.</p><p><strong>Methods: </strong>A liver cancer model was established in C57BL/6J mice, which were divided into a sham surgery group, a model control group, low-, medium- and high-dose curcumin groups, a STAT3 inhibitor group, a combination intervention group and a cisplatin positive control group. HE staining was used to observe the pathological morphology of liver tissue. Serum biochemical indicators and IL-6 and TGF-β1 levels were measured. STAT3 pathway proteins were detected by Western blot and related gene expression was analyzed by qRT-PCR.</p><p><strong>Results: </strong>Curcumin dose-dependently inhibited STAT3 signaling activity and reduced the p-STAT3/total STAT3 ratio (P<0.05). In the high-dose curcumin group, serum ALT, AST and TB levels decreased from (254.55±56.14)U/L, (542.01±71.28)U/L and (42.98±8.21)μmol/L to (47.98±10.68)U/L, (135.24±24.12)U/L and (7.91±1.36)μmol/L, respectively (all P<0.05). Curcumin upregulated the expression of BSEP, NTCP and FXR, downregulated Cyp7a1 and upregulated the expression of intestinal Fgf15 and Asbt.</p><p><strong>Conclusion: </strong>Curcumin alleviates liver injury and inflammation while ameliorating bile acid enterohepatic circulation by inhibiting the STAT3 signaling pathway.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"2199-2209"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-citrulline modulates pancreatic enzyme activity and biochemical markers in adult female Wistar rats with metabolic syndrome.","authors":"Adamu Imam Isa, Hanan Eissa, Mohamed-Nabih Abd-El Rahman, Nahid Ahmed Mohammed, Mohamed O'haj Mohamed","doi":"10.36721/PJPS.2026.39.7.209.1","DOIUrl":"10.36721/PJPS.2026.39.7.209.1","url":null,"abstract":"<p><strong>Background: </strong>Metabolic syndrome (MetS) induces profound pancreatic damage characterised by oxidative stress and chronic inflammation.</p><p><strong>Objectives: </strong>This study investigated the protective effects of L-citrulline (L-CIT) in the pancreas of a MetS animal model by evaluating oxidative stress responses, inflammatory cytokines, interferon gamma (IFN-γ), aldose reductase (AKR1B1), glucose kinase-3β, abdominal fat and body weight.</p><p><strong>Methods: </strong>The animals (Wistar rats; Rattus norvegicus) were divided into five groups (n = 6), three of which received MetS induction via a 20% fructose solution and received oral L-CIT at escalating doses (200, 400, or 800 mg/kg). Group 1 served as the normal control, while group 2 served as the negative control (20% fructose solution). Post-treatment, pancreas samples were collected for biochemical analysis to determine the dose-dependent effects of L-CIT.</p><p><strong>Results: </strong>Pancreatic oxidative stress was significantly reduced (P< 0.05) in the L-CIT-treated groups vs the MS-untreated group. TNF-ɑ, IL-1β and IL-6 were significantly reduced (P< 0.05) with L-CIT treatment vs the MS-untreated group. However, IL-10 was significantly increased (P < 0.05) in the L-CIT group compared with the MS-untreated group. Pancreatic IFN-γ was significantly (P< 0.05) reduced in the L-CIT groups compared to the MS-untreated group, whereas the changes in pancreatic BDNF were not statistically significant. Both pancreatic AKR1B1 and GSK-3β were significantly reduced (P< 0.05) in the groups given L-CIT vs the MS-untreated group. Abdominal fat and body weight were significantly lower (P< 0.05) in the L-CIT groups than in the MS-untreated group.</p><p><strong>Conclusion: </strong>L-CIT holds promise as a therapeutic supplement for preserving pancreatic health in metabolic disease.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"2218-2227"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine-coated hollow mesoporous silica nanoparticles loaded with lupeol regulates immune microenvironment in gastric cancer mice by down-regulating IL-10 through regulating NF-κb signaling.","authors":"Huijun Zhang, Xinyan Wei, Jun Guo, Jing Shu","doi":"10.36721/PJPS.2026.39.7.194.1","DOIUrl":"10.36721/PJPS.2026.39.7.194.1","url":null,"abstract":"<p><strong>Background: </strong>Modulating the immune microenvironment is a promising strategy against gastric cancer.</p><p><strong>Objectives: </strong>This study explored the mechanism of polydopamine-coated hollow mesoporous silica nanoparticles loaded with lupeol (Lupeol-PDA-HMSNs) in regulating the immune microenvironment via the NF-κB/IL-10 axis.</p><p><strong>Methods: </strong>Lupeol-PDA-HMSNs were synthesized and characterized. A gastric cancer mouse model was established and mice were randomly assigned to control, free Lupeol, PDA-HMSNs, or Lupeol-PDA-HMSNs groups. Tumor growth was monitored. Immune cell profiles and serum cytokines were assessed by ELISA and flow cytometry. Apoptosis-related proteins and NF-κB pathway markers were evaluated by RT-qPCR and Western blot. NF-κB p65 binding to the IL-10 promoter was examined by ChIP-qPCR.</p><p><strong>Results: </strong>Lupeol-PDA-HMSNs were successfully synthesized, exhibiting a uniform particle size of 24.85 ± 0.14 nm and a zeta potential of -21.8 mV. Compared to the NC group, Lupeol-PDA-HMSNs significantly inhibited tumor growth, showing superior efficacy to both free lupeol and the blank carrier (P<0.05). The treatment markedly reduced the proportion of Tregs, while enhancing the populations of CD3+CD4+ T cells and NK cells, as well as the CD4+/CD8+ ratio. Additionally, it up-regulated the expression of pro-apoptotic proteins Bax and Caspase-3, down-regulated the anti-apoptotic protein Bcl-2 and consequently promoted apoptosis. Furthermore, Lupeol-PDA-HMSNs suppressed the activity of the NF-κB pathway, leading to reduced expression of downstream inflammatory cytokines IL-6, IL-10 and TNF-α. ChIP-qPCR analysis confirmed that NF-κB p65 directly binds to the IL-10 promoter region (-1648 to -1638) and this binding was significantly attenuated by Lupeol-PDA-HMSNs treatment (P<0.01).</p><p><strong>Conclusion: </strong>Lupeol-PDA-HMSNs exert potent anti-tumor effects by promoting apoptosis and modulating the immune microenvironment through inhibition of the NF-κB/IL-10 pathway, highlighting their potential as a nanotherapeutic strategy for gastric cancer.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"2059-2070"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral osmotic pump drug delivery systems: A narrative review of structural designs, functional mechanisms and emerging applications.","authors":"Xintao Qiu, Jing Shu, Yuanyuan Chen, Qing Min, Yuting Bai, Hui Yao, Shuanglin Qin","doi":"10.36721/PJPS.2026.39.7.181.1","DOIUrl":"10.36721/PJPS.2026.39.7.181.1","url":null,"abstract":"<p><p>Traditional oral formulations often lead to significant fluctuations in plasma drug concentrations. In contrast, oral osmotic pumps achieve zero-order drug release driven by osmotic pressure, which is largely independent of physiological variables, offering an effective solution to optimize drug delivery outcomes. This review aims to summarize the structural types, functional mechanisms, and key factors influencing drug release of oral osmotic pumps, compare their performance disparities, and provide valuable references for subsequent research and development in this field. Oral osmotic pump systems were classified based on their structural designs, their mechanisms of action and key drug release-influencing factors were elaborated, and the characteristics and application potential of different systems were comparatively evaluated. Oral osmotic pump systems can be categorized into single-chamber, multi-chamber, and specific types, with push-pull osmotic pumps particularly suitable for poorly soluble drugs and targeted systems enabling site-specific therapy. Drug properties, osmotic pressure, semipermeable membrane characteristics, and release orifice parameters are critical factors governing drug release. Despite advantages such as stable release profiles and broad applicability, the system is limited by complex preparation processes and high costs. Oral osmotic pumps represent a highly valuable drug delivery technology. Future integration with intelligent technologies and advanced materials is expected to overcome existing challenges, facilitating more precise and efficient drug delivery.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"1901-1916"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of banana lectin from Musa paradisiaca and investigation of its interactions with selected anticancer drugs: Insights from spectroscopic, computational and cellular studies.","authors":"Rida Arif, Javeria Arif, Zoha Warsi, Syeda Sarah Tahir, Saima Rasheed","doi":"10.36721/PJPS.2026.39.7.188.1","DOIUrl":"10.36721/PJPS.2026.39.7.188.1","url":null,"abstract":"<p><strong>Background: </strong>Banana lectin (Banlec), a mannose/glucose-specific lectin isolated from Musa paradisiaca, is known for its immunomodulatory, antiviral and anticancer properties. However, its molecular interactions with anticancer drugs remain insufficiently explored, limiting its translational potential in targeted drug delivery.</p><p><strong>Objectives: </strong>This study aimed to purify Banlec and systematically investigate its binding interactions with selected FDA-approved anticancer drugs, dasatinib, 5-fluorouracil (5-FU) and anastrozole, to gain mechanistic insights into Banlec-drug recognition and stability.</p><p><strong>Methods: </strong>Banlec was purified from ripened Musa paradisiaca fruit using affinity chromatography. Binding interactions were examined using UV-Visible spectroscopy, circular dichroism (CD), differential scanning fluorimetry (DSF) and hemagglutination inhibition assay. Docking and molecular dynamics simulations were performed to analyze binding modes and complex stability. Additionally, the anticancer activity of Banlec and its drug-bound complexes was assessed against HL-60 cells using the MTT assay.</p><p><strong>Results: </strong>Banlec was purified from ripened Musa paradisiaca and characterized as a homodimeric protein (~30 kDa). UV-Visible spectroscopy confirmed spontaneous Banlec-drug interactions. CD analysis showed that Banlec retained its secondary structure upon binding with dasatinib and 5-FU, whereas pronounced conformational changes were observed with anastrozole. DSF analysis showed slight thermal stabilization with dasatinib and 5-FU, while anastrozole caused thermal destabilization. Hemagglutination inhibition assays indicated that the selected drugs did not mask the Banlec carbohydrate-binding domains and therefore its glycan-targeting potential is retained. Docking and MD simulations showed strongest binding with dasatinib, providing maximal stabilization, followed by 5-FU with moderate stabilization. Notably, among the evaluated complexes, Banlec-5-FU complex exhibited the most potent anticancer activity against HL-60 cells, compared to Banlec and 5-FU alone.</p><p><strong>Conclusion: </strong>This study provides the first comprehensive insight into Banlec-anticancer drug interactions, identifying 5-FU as the most favorable binding partner. The findings highlight Banlec as a promising scaffold for development of glycan-targeted anticancer drug delivery systems and expand its therapeutic potential.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"1977-1992"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity based insights into the seed proteins of Datura alba from Pakistan.","authors":"Tehreem Abdul Hameed, Laraib Batool, Ifrah Siddiqui, Summra Ahmed, Nida Dastagir, Humera Waheed, Syed Faraz Moin","doi":"10.36721/PJPS.2026.39.7.201.1","DOIUrl":"10.36721/PJPS.2026.39.7.201.1","url":null,"abstract":"<p><strong>Background: </strong>There are fifteen species of Datura, a perennial shrub-like herb, with Datura alba (D. alba) being the most important medicinal plant because of its therapeutic and aesthetic uses. Pharmaceutical goods use fresh seeds, fresh leaves, roots, dried leaves, dried mature seeds and fruits of D. alba. Seed proteins are the plant component most often overlooked for use in complementary and alternative medicine, even though all plant parts have nearly identical therapeutic properties and effects. This results from a deficiency of knowledge on the components of proteins.</p><p><strong>Objectives: </strong>The current study is to extract proteins from D. alba plant seeds in order to show data that will identify few major enzymes in the whole protein extract.</p><p><strong>Methods: </strong>The protein chemistry techniques, such as gel electrophoresis and chromatography, have been applied for seed protein analysis. After extraction of seed proteins in PBS (pH 7.4) buffer, the chromatography using DEAE Sephacryl (macroprep) resin, SDS-PAGE, zymography and biochemical tests were performed.</p><p><strong>Results: </strong>There were three distinct peaks identified by the chromatographic separation. Protein bands were visible in the reduced and non-reduced SDS-PAGE analysis, while the results of substrate zymography indicated the presence of proteases and phospholipases. Strong antioxidant activity and antibiofilm activity on MRSA were also observed in the D. alba seeds proteins.</p><p><strong>Conclusion: </strong>Enzymatic and biochemical activities were observed with the protein extract obtained from the seeds of D. alba. Fractions of the proteins were also active and showed separation of protein components on the basis of ionic strength.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"39 7","pages":"2127-2137"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}