Molecular characterization of extensively drug-resistant Salmonella serovar Typhi in patients with gastrointestinal complications in Quetta, Pakistan.

IF 0.7 4区医学 Q4 PHARMACOLOGY & PHARMACY

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI:10.36721/PJPS.2025.38.4.REG.14048.1

Hafsah Tariq, Abdul Samad, Saadullah Jan, Hafiz Muhammad Mazhar Asjad, Jawad Akbar Khan, Farah Sabeen Bugti, Muhammad Naeem, Abdul Rahim Bugti, Adnan Ayoub

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Abstract

Extensive drug resistance (XDR) S. typhi have been evaluated in patients with gastrointestinal disturbance who attended multiple tertiary care hospitals in Quetta, Balochistan, Pakistan. Blood samples of total of 480 patients were obtained and S. typhi was isolated and verified by PCR. Isolates were subjected to antimicrobial susceptibility testing and antimicrobial resistance (AMR) genes of 1^st, 2^nd line antibiotics, 3^rd generation cephalosporin and azithromycin were identified by PCR. Among 65 PCR confirmed S. typhi cases, 18(27%) were Multidrug resistance (MDR), 25(38%) XDR, 13 (20%) Extended spectrum β-lactamase (ESBL) and only 4(6%) Azithromycin-resistant XDR S. typhi. The high frequency was observed for the antibiotics-resistant genes catA1, bla_TEM-1 (100%), dhfr7 (95%), sul1 (98%), gyrA, gyrB, parC (93%) and qnrS and parE 100% each. The frequency of bla_CTX-M-15 and acrB were 78% and 6% respectively. We found high burden of MDR, XDR and ESBLs S. typhi. The AMR genes were similar to those of the regional countries. Azithromycin resistance was low could be a drug of choice against XDR S. typhi in the study area. The study provided the molecular profile of AMR S. typhi in Quetta, capital of Balochistan province of Pakistan.

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巴基斯坦奎达胃肠并发症患者中广泛耐药伤寒沙门氏菌的分子特征

在巴基斯坦俾路支省奎达多家三级保健医院就诊的胃肠道紊乱患者中，对伤寒沙门氏菌广泛耐药（XDR）进行了评估。共采集480例患者血样，分离出伤寒沙门氏菌，并进行PCR验证。对分离株进行药敏试验，用PCR方法鉴定1、2线抗生素、3代头孢菌素和阿奇霉素的耐药基因。65例经PCR确诊的伤寒沙门氏菌中，多药耐药（MDR） 18例（27%），XDR 25例（38%），扩展谱β-内酰胺酶（ESBL） 13例（20%），阿奇霉素耐XDR伤寒沙门氏菌4例（6%）。耐药基因catA1、blaem -1（100%）、dhfr7（95%）、sul1（98%）、gyrA、gyrB、parC（93%）、qnrS和parE（100%）出现频率较高。blaCTX-M-15和acrB的检出率分别为78%和6%。我们发现耐多药、广泛耐药和ESBLs伤寒链球菌的负担很高。AMR基因与该地区国家的基因相似。阿奇霉素耐药较低，可作为广泛耐药伤寒沙门氏菌的首选药物。该研究提供了巴基斯坦俾路支省首府奎达的AMR S.伤寒的分子图谱。

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来源期刊

Pakistan journal of pharmaceutical sciences 医学-药学

CiteScore

1.40

自引率

12.50%

发文量

211

审稿时长

4.5 months

期刊介绍： Pakistan Journal of Pharmaceutical Sciences (PJPS) is a peer reviewed multi-disciplinary pharmaceutical sciences journal. The PJPS had its origin in 1988 from the Faculty of Pharmacy, University of Karachi as a biannual journal, frequency converted as quarterly in 2005, and now PJPS is being published as bi-monthly from January 2013. PJPS covers Biological, Pharmaceutical and Medicinal Research (Drug Delivery, Pharmacy Management, Molecular Biology, Biochemical, Pharmacology, Pharmacokinetics, Phytochemical, Bio-analytical, Therapeutics, Biotechnology and research on nano particles.