Pakistan journal of pharmaceutical sciences最新文献_第9页

Effect of multicomponent crystal of piperine-nicotinic acid on antihyperlipidemic activity in rats. 哌啶-烟酸多组分晶体对大鼠降血脂活性的影响

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-11-01

Maria D Octavia, Hasmiwati Hasmiwati, Gusti Revilla, Erizal Zaini

{"title":"Effect of multicomponent crystal of piperine-nicotinic acid on antihyperlipidemic activity in rats.","authors":"Maria D Octavia, Hasmiwati Hasmiwati, Gusti Revilla, Erizal Zaini","doi":"","DOIUrl":"","url":null,"abstract":"The alkaloid piperine is the main bioactive compound in black pepper (Piper nigrum L) and exhibits antihyperlipidemic activity. Piperine is a Biopharmaceutical Classification System (BCS) Class II compound, which has low water solubility, resulting in low bioavailability. This study aims to examine the effects of multicomponent nicotinic acid-piperine crystals on antihyperlipidemic activity in rats fed a high-fat diet. To increase the effectiveness of piperine, we prepared multicomponent crystals by the solvent-drop grinding method, using nicotinic acid as a co-former. The antihyperlipidemic activity of the preparation was estimated by measuring total cholesterol (TC), total triglycerides (TG), low-density lipoprotein cholesterol (LDLc) and high-density lipoprotein cholesterol (HDLc), using the enzymatic colorimetric method. Rats fed a high-fat diet exhibited an increase in plasma lipid levels. However, rats administered the multicomponent piperine-nicotinic acid crystals at a dose of 40 mg/kg/BW showed significantly (p<0.050) reduced plasma lipid levels. Compared with hyperlipidemic rats, multicomponent crystals of piperine-nicotinic acid decreased TC from 237.8±8.02 mg/dL to 174.53±7.07mg/dL, TG levels from 208.33±5.79 to 85.95±7.41mg/dL and LDLc levels from 144.225±15.99 mg/dL to 88.55±10.83mg/dL but increased HDLc levels from 51.93±10.92mg/dL to 68.78±2.56 mg/dL. Thus, the results demonstrate that the multicomponent piperine-nicotinic acid crystals lowered TC, TG and LDLc but increased HDLc.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium p-hydroxybenzoate alleviates osteoporosis through inhibiting bone metabolism and oxidative stress via activating ERα. 对羟基苯甲酸钠通过激活ERα抑制骨代谢和氧化应激，减轻骨质疏松症。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Xiaotian Xu, Huideng Wang, Xi Lu, Miaozhen Fan, Ailin Luo, Meng Liu, Yuhui Wang, Xiaoqun Duan

{"title":"Sodium p-hydroxybenzoate alleviates osteoporosis through inhibiting bone metabolism and oxidative stress via activating ERα.","authors":"Xiaotian Xu, Huideng Wang, Xi Lu, Miaozhen Fan, Ailin Luo, Meng Liu, Yuhui Wang, Xiaoqun Duan","doi":"","DOIUrl":"","url":null,"abstract":"As the population ages, the incidence of osteoporosis (OP) gradually increases and is becoming a growing public health problem. Meanwhile, although traditional pharmacological therapy is extremely efficient in the treatment of OP, its application is constrained because of irreversible adverse drug reactions. Therefore, scientists should actively develop safer drugs while ensuring the therapeutic effect of OP. Previous studies have shown that p-hydroxybenzoic acid (HA) can upregulate the expression of estrogen receptor (ER). Sodium p-hydroxybenzoate (DSN160) is a sodium salt of HA with a lethal dose greater than 5g/kg. However, whether DSN160 has demonstrable anti-osteoporotic activities remains unclear. In this study, DSN160 increased the organ index, length and diameter of the bone and bone mineral density and improved bone microstructure in retinoic acid-induced OP rats. Furthermore, DSN160 reduced bone metabolism-related indicators. In addition, fulvestrant (a specific antagonist of ER) blocked the anti-OP effect of DSN160. In conclusion, our findings showed that DSN160 exerts anti-OP effect through inhibiting bone metabolism and oxidative stress via activating ERα.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49691928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antinociceptive effects of carbidopa levodopa on normal rats and Parkinson's disease mice. 卡比多巴左旋多巴对正常大鼠和帕金森病小鼠的抗伤害作用。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Zhang Wenbo, Ma Zhigang, Zhang Jiren, Zhang Ce, Li Ting, Zhang Pinyuan, Wang Tian

{"title":"Antinociceptive effects of carbidopa levodopa on normal rats and Parkinson's disease mice.","authors":"Zhang Wenbo, Ma Zhigang, Zhang Jiren, Zhang Ce, Li Ting, Zhang Pinyuan, Wang Tian","doi":"","DOIUrl":"","url":null,"abstract":"Carbidopa levodopa is widely used to ameliorate motor symptoms of Parkinson's disease (PD) patients. Pain is one of common symptoms of PD. The aim of this experiment is to study antinociceptive effects of carbidopa levodopa on normal rats and PD mice. Rats were intragastrically treated with carbidopa levodopa and the hind paw withdrawal latency (HWL) was investigated. PD mouse model was prepared with MPTP and then the antinociceptive effects of carbidopa levodopa on PD mice were evaluated. In normal rats, the HWL to thermal stimulus was augmented after carbidopa levodopa administration (p<0.05 or p<0.01) and carbidopa levodopa increased the HWL (p<0.05 or p<0.01) to mechanical stimulus. In PD mice, carbidopa levodopa elevated the HWL of the thermal stimulus in PD mice (p<0.05). Furthermore, the HWL in the inflammatory pain of PD mice was also increased by carbidopa levodopa treatmet (p<0.01). The current findings indicate that carbidopa levodopa has an antinociceptive effects in normal rats and PD mice. The analgesic effect of carbidopa levodopa on patients with or without PD is worth studying in further research.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49691827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a validated RP-HPLC method for rivaroxaban quantification in pharmaceutical formulation and human blood plasma. 开发一种有效的RP-HPLC方法来定量药物制剂和人血浆中的利伐沙班。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Muhammad Jahangir, Aymen Awan, Arooj Azhar

{"title":"Development of a validated RP-HPLC method for rivaroxaban quantification in pharmaceutical formulation and human blood plasma.","authors":"Muhammad Jahangir, Aymen Awan, Arooj Azhar","doi":"","DOIUrl":"","url":null,"abstract":"Rivaroxaban is an anticoagulant used to prevent thromboembolism after hip or knee joint replacement surgery. The purpose of study was the development of an efficient, simple and economic reverse phase HPLC-PDA method for rivaroxaban determination in pharmaceutical dosage forms and blood plasma of human beings. The separation was carried out at room temperature by using Thermo Scientific ODS Hypersil C18 (250×4.6mm, 5µm) and mobile phase 70:30 (%v/v) mixture of ACN / H2O, 1.2 ml/min flow rate, detection on 253nm wavelength by PDA detector with run time of about 7 mins. The retention time observed was about 3 mins. The validation was performed on the proposed method in accordance with ICH guidelines and found that the method is linear within the range of 100-400µg/mL with the correlation coefficient 0.9996. The method was also precise, accurate, robust and rugged and showed specificity in all applied stress conditions i.e. photolytic (200-800nm, 3h) thermal (70°C, 2h), oxidative (3% H2O2 , 70°C, 1h), acidic (0.1 N, 70°C, 1h), and basic (0.1 N, 60°C, 1h). This method was applied successfully for the Rivaroxaban quantitative determination in pharmaceutical dosage and human blood plasma.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49691831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sericin suppresses high glucose-induced EMT in mouse podocytes via miR-30a-5p and its target Snai1. 丝氨酸蛋白酶通过miR-30a-5p及其靶标Snai1抑制小鼠足细胞中高糖诱导的EMT。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Donghui Liu, Cheng Chen, Ting Ge, Zhihong Chen

{"title":"Sericin suppresses high glucose-induced EMT in mouse podocytes via miR-30a-5p and its target Snai1.","authors":"Donghui Liu, Cheng Chen, Ting Ge, Zhihong Chen","doi":"","DOIUrl":"","url":null,"abstract":"The effect of sericin in high glucose (HG)-induced podocyte injury and the mechanisms involving Snai1 and miR-30a-5p were investigated. Bioinformatics and dual-luciferase reporter assay evaluated the relationship of Snai1 with miR-31a-5p. Podocyte injury mouse induced by HG were randomly divided into control (5.5mmol/L D-glucose), HG (30mmol/L D-glucose), HG + Sericin (30mmol/L D-glucose+600μg/ml sericin), miR-30a-5p inhibitor NC (sericin+30mmol/L D-glucose+miR-30a-5p inhibitor negative control) and miR-30a-5p inhibitor groups (sericin+30mmol/L D-glucose+miR-30a-5p inhibitor). The migration ability of podocytes was detected by Transwell assay. The expressions of Snai1, podocin, E-cadherin, FSP-1, ZO-1, α-SMA, Desmin, and miR-30a-5p were assessed with RT-qPCR and Western blot. Snai1 was one direct target of miR-30a-5p. HG group had significantly larger number of migrated podocytes and higher levels of Snai1, FSP-1, α-SMA and Desmin, but significantly lower levels of podocin, ZO-1 and E-cadherin than control and HG + Sericin group. These effects of sericin were reversed by miR-30a-5p inhibitor, as evidenced by increased podocyte migration and increased expressions of Snai1, α-SMA, FSP-1 and Desmin, whereas decreased expressions of podocin, ZO-1 and E-cadherin. Sericin may protect podocytes from damage caused by HG via up-regulating epithelial phenotype markers, down-regulating mesenchymal phenotype markers, and reducing migration of podocytes. The mechanism may be through targeting miR-30a-5p and its target Snai1.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49691927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of letrozole-encapsulated polymeric beads for sustained release. 来曲唑包封缓释聚合物微球的研制与表征。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Muhammad Abu Sufian, Syed Atif Raza, Humayun Riaz, Waqas Ahmad, Zia Mohy Uddin Khan, Hammad Yousuf, Muhammad Affan, Ayesha Ghafoor, Adeel Masood Butt, Muhammad Umar Farooq, Wajeeha Akram

{"title":"Development and characterization of letrozole-encapsulated polymeric beads for sustained release.","authors":"Muhammad Abu Sufian, Syed Atif Raza, Humayun Riaz, Waqas Ahmad, Zia Mohy Uddin Khan, Hammad Yousuf, Muhammad Affan, Ayesha Ghafoor, Adeel Masood Butt, Muhammad Umar Farooq, Wajeeha Akram","doi":"","DOIUrl":"","url":null,"abstract":"The study aimed to prepare and characterize biodegradable sustained-release beads of letrozole (LTZ) for treating cancerous disease. The ionotropic gelation method was used for the preparation and calcium chloride (CaCl2) was used as a gelating agent, while chitosan (CTS) and sodium alginate (NaAlg) as biodegradable polymeric matrices in the blend hydrogel beads. The beads were characterized for their size, surface morphology, drug entrapment efficiency, drug-polymer interaction and crystallinity using different analytic techniques, including optical microscopy, Scanning Electron Microscopy (SEM), UV-spectroscopy, Fourier-transform Infrared Spectroscopy (FTIR), Thermo gravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC) and X-ray Diffraction Analysis (XRD) respectively. In vitro swelling studies were also applied to observe the response of these polymeric networks against different pH (at 1.2, 6.8 and 7.4 pH). The results from TGA and DSC exhibited that the components in the formulation possess better thermal stability. The XRD of polymeric networks displays a minor crystalline and significant amorphous nature. The SEM micrographs revealed that polymeric networks have uneven surfaces and grooves. Better swelling and in vitro outcomes were achieved at a high pH (6.8,7.4), which endorsed the pH-responsive characteristics of the prepared beads. Hence, beads based on chitosan and sodium alginate were successfully synthesized and can be used for the controlled release of letrozole.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of Moringa oleifera and Allium sativum on BMI and lipid profile: A randomized controlled trial. 辣木和葱对BMI和血脂的增效作用:一项随机对照试验。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Amna Sarfraz, Muhammad Imran Hussain, Ramisha Ibtisam, Annum Maha, Saher Asghar, Khizra Zubair, Khurram Sarfraz

{"title":"Synergistic effect of Moringa oleifera and Allium sativum on BMI and lipid profile: A randomized controlled trial.","authors":"Amna Sarfraz, Muhammad Imran Hussain, Ramisha Ibtisam, Annum Maha, Saher Asghar, Khizra Zubair, Khurram Sarfraz","doi":"","DOIUrl":"","url":null,"abstract":"We investigate the synergistic effect of Moringa oleifera and Allium sativum on hyperlipidemic patients. It was a randomized controlled trial. The sample size of this study was 60 patients who were divided into four equal groups (n=15) through a random sampling technique. Pre assessment of all participants was conducted at the start of the research. The control group (group A) received atorvastatin 10mg QD as well as placebo capsules. The treatment group 1 (group B) received atorvastatin 10mg QD as well as Moringa oleifera 2g (capsule 1g BID). Treatment group 2 (group C) received atorvastatin 10mg QD as well as Allium sativum 2g (capsule 1g BID). Lastly, treatment group 3 (Group D) received atorvastatin 10mg QD as well as combined Moringa oleifera 2g (capsule 1g BID) and Allium sativum 2g (capsule 1g BID). After an intervention of 45 days, post assessment was conducted. The results showed total cholesterol and triglycerides and low-density lipoprotein of participants of treatment group 3 who received both MO and AS were statistically significant (p<0.05). The group that was on Moringa oleifera supplements alone was found to be statistically significant (p<0.05). This study concluded that Moringa oleifera and Allium sativum considerably improved the BMI and lipid profile of participants.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of Hippophae rhamnoides L. on 5-fluorouracil-ınduced oral mucositis in rats. 沙棘对5-氟尿嘧啶诱导的大鼠口腔粘膜炎的作用。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Muhammet Dilber, Bahadir Suleyman, Renad Mammadov, Zeynep Suleyman, Bulent Yavuzer, Mine Gulaboglu, Ertugrul Erhan, Ismail Salcan, Halis Suleyman

{"title":"The role of Hippophae rhamnoides L. on 5-fluorouracil-ınduced oral mucositis in rats.","authors":"Muhammet Dilber, Bahadir Suleyman, Renad Mammadov, Zeynep Suleyman, Bulent Yavuzer, Mine Gulaboglu, Ertugrul Erhan, Ismail Salcan, Halis Suleyman","doi":"","DOIUrl":"","url":null,"abstract":"Current study aimed to research the effect of Hippophae rhamnoides (HRE) on potantial oral oxidative and inflammatory damage of 5-FU in rats. The rats were assigned to three groups; healthy (HG), 5-FU 100mg/kg (FUG) and HRE 50mg/kg +5-FU 100mg/kg (HRFU). The 5-FU was injected in the FUG group intraperitoneally. The HRFU was injected 5-FU at 100mg/kg IP one hour after the 50mg/kg HRE was given orally. Olive oil was used as a solvent for the HG. HRE was given to the rats three times a day for ten days. 5-FU was given one dose on the 1st, 3rd and 5th days. On the 10th day, the tissues removed from the animals were euthanized with high-dose anaesthesia and were macroscopically examined. The levels of the oxidant, antioxidant and proinflammatory cytokines were investigated.It was seen that HRE alleviated the symptoms of severe mucositis by antagonizing the effects of 5-FU on oxidant, antioxidant and proinflammatory cytokines such as malondialdehyde, total glutathione, superoxide dismutase, catalase, nuclear factor kappa-B and interleukin-6 in inner cheek and tongue tissue. These results recommend that HRE may be benefical in the cure of 5-FU-associated oral mucositis.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49691930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination therapy of methotrexate and iguratimod for female patients with moderate to severe active rheumatoid arthritis and its impact on ovarian function. 甲氨蝶呤与依瓜拉莫特联合治疗女性中重度活动性类风湿关节炎及其对卵巢功能的影响。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Zhang Jingzhou, Zhou Xiong, Kong Qing Xin

{"title":"Combination therapy of methotrexate and iguratimod for female patients with moderate to severe active rheumatoid arthritis and its impact on ovarian function.","authors":"Zhang Jingzhou, Zhou Xiong, Kong Qing Xin","doi":"","DOIUrl":"","url":null,"abstract":"Rheumatoid arthritis is systemic inflammatory disease, presenting as symmetrical polyarthritis, mainly affecting hands and feet. Female patients with rheumatoid arthritis have also decreased fertility and premature ovarian failure. Methotrexate is used in treatment of rheumatoid arthritis. However, single treatment of Methotrexate is not ideal and often needs to be combined with other drugs like Iguratimod for intensive treatment of refractory rheumatoid arthritis. We aim to explore efficacy of combined use of MTX and IGU for female patients with rheumatoid arthritis and its impact on ovarian reserve function. Total 80 female patients with moderate to severe active rheumatoid arthritis were selected and randomly divided into MTX and MTX + IGU groups. The MTX + IGU group showed lower levels of IL-1β, IL-6, IL-17 and TNF-α after treatment as compared to MTX group. The MTX + IGU group showed significantly higher level of AMH at the end of treatment. However, no statistical differences were observed in FSH, LH, E2 and AFC levels between the two groups. The combined therapy of MTX and IGU has better efficacy with less impact on ovarian function, even improving ovarian reserve function through effective control of rheumatoid inflammatory activity.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of polyamidoamine based doxorubicin loaded polymeric nanocarriers and in-vitro evaluation on liver cancer cell lines. 聚酰胺胺基多柔比星负载聚合物纳米载体的研制、表征及对肝癌细胞系的体外评价。

IF 0.8 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2023-09-01

Nadia Mazhar, Zeeshan Danish, Hamid Saeed

{"title":"Development and characterization of polyamidoamine based doxorubicin loaded polymeric nanocarriers and in-vitro evaluation on liver cancer cell lines.","authors":"Nadia Mazhar, Zeeshan Danish, Hamid Saeed","doi":"","DOIUrl":"","url":null,"abstract":"In order to achieve the benefits of targeted drug delivery, this study intended to encapsulate doxorubicin in a linear polyamidoamine and its PEGylated co-polymer. The drug was loaded by using the emulsion solvent evaporation method. By adjusting the doxorubicin to polymer ratios to 1:10, 1:20 and 1:30, three formulations of each polymer/copolymer were prepared. The drug release profile was investigated using phosphate buffered saline. In vitro cytotoxicity investigation was executed on liver cancer cell line (Hep G2 cell lines) by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The outcome demonstrated that doxorubicin had been successfully loaded on polyamidoamine and its PEGylated co-polymer with a drug loading efficiency of about 90%. Nanocarrier sizes were between 245±1.10 nm -579±1.00 nm and the zeta potential range was +22.4±0.5 mV-+37.9±0.3 mV. In-vitro drug release investigations revealed a characteristic pH-dependent drug release. The cytotoxicity testing of optimal formulation revealed that the doxorubicin was successfully released from the formulations and exerted therapeutic effect. According to our research, doxorubicin could be loaded onto linear polyamidoamines for potent antitumor effects on the target liver cancer cell lines (Hep G2).","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0