Pakistan journal of pharmaceutical sciences最新文献_第9页

Evaluation of metformin use in non-critically hospitalized patients at a single tertiary hospital in Saudi Arabia: A cross-sectional study. 评价二甲双胍在沙特阿拉伯一家三级医院的非危重住院患者中的使用：一项横断面研究。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13012.1

Mohannad Alshibani, Samah Alshehri

{"title":"Evaluation of metformin use in non-critically hospitalized patients at a single tertiary hospital in Saudi Arabia: A cross-sectional study.","authors":"Mohannad Alshibani, Samah Alshehri","doi":"10.36721/PJPS.2025.38.4.REG.13012.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.13012.1","url":null,"abstract":"The aim of this study was to assess the safety of metformin use in non-critically hospitalized patients. A cross-sectional study was performed at a single tertiary hospital in Saudi Arabia between January 2019 and June 2019. Patients aged >18 years with a confirmed diagnosis of type 2 diabetes who were taking metformin prior to admission were included. The primary outcome was the appropriateness of metformin use in non-critically hospitalized patients. A total of 251 patients were included in this study. Metformin was appropriate in 96 patients (38.2%). There were 14 (5.6%) incidences of absolute contra-indications and 141 (56.1%) incidences of precautions. Non-Saudi patients were about 1.856 times more likely to receive an inappropriate metformin dosage regimen compared to Saudi patients (AOR= 1.856; P= 0.022, 95% CI= 1.093, 3.151). Additionally, patients with chronic kidney disease (stage 3A) were approximately 2 times less likely to receive an inappropriate metformin dosage regimen than patients on stage 5 (AOR= 0.482; P= 0.035, 95% CI= 0.243, 0.835). This study highlighted a high rate of inappropriate metformin use in non-critically hospitalized patients. However, patients who were non-Saudi and at an advanced stage of chronic kidney disease were more likely to receive an inappropriate metformin dosage regimen.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1347-1353"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel strategy for AAV vector design in gene therapy and its biosafety assessment. AAV基因治疗载体设计新策略及其生物安全性评价。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13523.1

Caihong Gao

{"title":"Novel strategy for AAV vector design in gene therapy and its biosafety assessment.","authors":"Caihong Gao","doi":"10.36721/PJPS.2025.38.4.REG.13523.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.13523.1","url":null,"abstract":"To develop a novel AAV vector design for gene therapy by synthesizing the AAVITR microcarrier sequence, constructing and identifying the amplification plasmid, and evaluating the properties of the microcarrier prepared by heat denaturation. We also assess the biosafety of this new vector by examining its toxicity, immunogenicity, and potential for immune cell infiltration. The AAVITR micro carrier sequence was designed and synthesized, followed by the construction of an amplification plasmid to express the vector sequence and the identification of pUC57-minivector. The in vitro expression of the AAVITR micro carrier was analyzed and its safety was assessed through toxicity, immunogenicity, and immune cell infiltration assays. In safety assessments, the new vector demonstrated superior survival and apoptosis rates compared to conventional vectors. Additionally, the AAV-ITR micro carrier showed significant advantages in immune cell infiltration, cytokine levels, and antibody production, indicating a potent immune response. The novel AAVITR micro vector developed in this study offers improved safety and efficacy in gene therapy, with notable advantages in immune response and biosafety, providing a promising alternative to traditional AAV vectors.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1174-1183"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated in vivo and in silico ADMET and metabolomic profiling of basil seed bioactives Methyl eugenol and linalool. 罗勒种子生物活性物质甲基丁香酚和芳樟醇的体内和体外ADMET和代谢组学分析。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.14703.1

Mahpara Gondal, Muhammad Sajid Hamid Akash, Kanwal Rehman

{"title":"Integrated in vivo and in silico ADMET and metabolomic profiling of basil seed bioactives Methyl eugenol and linalool.","authors":"Mahpara Gondal, Muhammad Sajid Hamid Akash, Kanwal Rehman","doi":"10.36721/PJPS.2025.38.4.REG.14703.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.14703.1","url":null,"abstract":"Biotransformation pathways critically predict rational drug design by elucidating a compound's absorption, distribution, metabolism, excretion and toxicity (ADMET) profile. This investigation provides a consolidated in vivo and in silico assessment of methyl eugenol (ME) and linalool (LL). Acute oral toxicity studies in Swiss albino rats revealed no mortality or clinical distress up to 2000 mg/kg. Noncompartmental pharmacokinetic analysis showed both compounds reached maximum plasma concentration at 4 hr (413.20 ng/mL for ME; 248.66 ng/mL for LL) and were largely cleared within 24 hrs. Despite identical Tmax values, their elimination half-lives differed significantly (30.0 h for ME vs. 117.5 h for LL), leading to greater systemic exposure for LL. Time-resolved LC-MS/MS identified distinct phase I metabolic pathways for each compound, which were corroborated by in silico predictions. Molecular docking against α-amylase and acetylcholinesterase, indicated favorable binding energies for both compounds, with ME showing slightly stronger affinity in some instances (e.g., -5.6 vs. -5.0 kcal/mol for α-amylase). However, LL consistently exhibited lower RMSD values, suggesting more specific binding. This integrated empirical-computational approach offers a foundational ADMET profile, guiding future structural modifications to optimize their therapeutic potential.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1254-1271"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of biofilm formation in methicillin resistant Staphylococcus aureus isolates by genotypic and phenotypic methods and effect of vancomycin and teicoplanin on biofilm inhibition. 用基因型和表型方法研究耐甲氧西林金黄色葡萄球菌分离株生物膜的形成及万古霉素和替柯planin对生物膜的抑制作用。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13543.1

Zeliha Seyfi Sanda, Demet Gur Vural, Asuman Birinci

{"title":"Investigation of biofilm formation in methicillin resistant Staphylococcus aureus isolates by genotypic and phenotypic methods and effect of vancomycin and teicoplanin on biofilm inhibition.","authors":"Zeliha Seyfi Sanda, Demet Gur Vural, Asuman Birinci","doi":"10.36721/PJPS.2025.38.4.REG.13543.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.13543.1","url":null,"abstract":"The pathogenicity factors of Staphylococcus aureus include biofilm production. In this study, the biofilm production abilities of methicillin-resistant S. aureus (MRSA) strains were investigated using genotypic and phenotypic methods. Additionally, the effect of glycopeptides on biofilm was examined. This study included 130 MRSA isolates. Biofilm was detected by the microtiter plate method. The minimum inhibitory concentration (MIC) of glycopeptides was evaluated through the broth microdilution method. The biofilm inhibitor concentration (BIC) values were investigated in isolates with strong biofilm production. The mecA (methicillin resistance gene), icaA, and icaD (biofilm-associated genes) were amplified by polymerase chain reaction techniques. Eighty-one isolates (62.31%) formed biofilms, while thirty isolates (23.08%) exhibited strong biofilm formation. Thirty isolates had higher BIC90 values than MIC90 values. The mecA gene was confirmed in 125 (96.15%) isolates, the icaA gene in 96 (73.85%) isolates, and the icaD gene in 100 (76.92%) isolates. There was statistical significance between ica genes and the biofilm produced (p<0.05). In conclusion, increased biofilm formation due to the effect of ica genes increases the concentration values at which antibiotics act.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1133-1140"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HPLC fingerprinting-guided determination of the optimal harvest period for Jinsi Huangju (Chrysanthemum morifolium Ramat.) and to elucidate its anti-inflammatory mechanisms. HPLC指纹图谱法确定金丝黄菊最佳采收期并探讨其抗炎作用机制。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.14233.1

Hualin Hu, Li Guo, Jian Fan, Fuxiao Wei, Xing Yu, Jianguo Cen

{"title":"HPLC fingerprinting-guided determination of the optimal harvest period for Jinsi Huangju (Chrysanthemum morifolium Ramat.) and to elucidate its anti-inflammatory mechanisms.","authors":"Hualin Hu, Li Guo, Jian Fan, Fuxiao Wei, Xing Yu, Jianguo Cen","doi":"10.36721/PJPS.2025.38.4.REG.14233.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.14233.1","url":null,"abstract":"'Jinsi Huangju' (JSHJ, Chrysanthemum morifolium Ramat.) is a medicinal plant traditionally used to manage inflammation. However, its optimal harvesting period and underlying anti-inflammatory mechanisms remain insufficiently explored. Therefore, this study aimed to investigate the effects of three JSHJ harvesting stages-initial flowering (CH), full flowering (SH), and final flowering (ZH)-on the bioactive compounds and to elucidate its anti-inflammatory mechanisms. The relative content of bioactive compounds (3,5-O-dicaffeoylquinic acid, chlorogenic acid, galuteolin) at each harvesting stage was determined using High-Performance Liquid Chromatography (HPLC, 1260, Agilent) fingerprints. The similarity of these fingerprints was then evaluated using the Chromatographic Fingerprint Similarity Evaluation System for Chinese Medicine (2004A edition). Anti-inflammatory activity was assessed in LPS-stimulated RAW 264.7 macrophages through MTT, Griess, and ELISA assays. The SH stage maximized JSHJ biomass and bioactive compounds. JSHJ extracts (20-50 µg/mL) significantly reduced LPS-induced levels of NO (from 9.45 to 0.16 µmol/L), TNF-α (from 127.26 to 8.09 pg/mL), and ROS. Among the extracts, petroleum ether (JSHJ-3) and ethyl acetate (JSHJ-4) exhibited the strongest anti-inflammatory effects. This study demonstrates that the SH stage is the most favorable harvest period for JSHJ, with extracts, particularly JSHJ-3/4, showing significant anti-inflammatory activity. These findings underscore the potential of JSHJ extracts as natural anti-inflammatory agents.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1272-1284"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of health status of sepsis patients undergoing blood purification therapy by using combination of acetylcysteine and frail management. 乙酰半胱氨酸联合虚弱管理对血液净化治疗脓毒症患者健康状况的改善。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13502.1

Jun Wu, Li Wang, Xiaorong Cheng

{"title":"Improvement of health status of sepsis patients undergoing blood purification therapy by using combination of acetylcysteine and frail management.","authors":"Jun Wu, Li Wang, Xiaorong Cheng","doi":"10.36721/PJPS.2025.38.4.REG.13502.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.13502.1","url":null,"abstract":"In this study, the efficacy of Fatigue, Resistance, Ambulation, Illness and Loss of weight (FRAIL) management combined with acetylcysteine (AChE) in blood purification therapy for sepsis (SS) patients was investigated. Seventy-eight SS patients undergoing blood purification were randomized to an observation group (FRAIL + AChE, n=42) or a control group (conventional care, n=36). FRAIL score, APACHE II score, vital signs (heart rate, mean arterial pressure, oxygen saturation), inflammatory factors oxidative stress indexes and nutritional proteins were assessed before and after the intervention in both groups. The results of the study showed that after the intervention, the FRAIL score and APACHE II score were reduced more significantly in the observation group (P<0.05). In addition, vital signs improved more significantly in the observation group. After the intervention, all inflammatory factors in the observation group were lower than those in the control group, while nutritional proteins were higher than those in the control group (P<0.05). In addition, oxidative stress injury was also more favorable in the observation group than in the control group after the intervention. These results suggest that FRAIL management combined with AChE may play a significant role in SS treatment.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1125-1132"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach to urease inhibition and antioxidant therapeutics by using AgNPs-doped TiO₂NPs stabilized by short chain pyrazole carbothioamide derivatives. 利用短链吡唑碳硫酰胺衍生物稳定的agnps掺杂TiO2NPs进行脲酶抑制和抗氧化治疗的新方法。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13601.1

Rafia Usman Khan, Faiza Saleem, Mehreen Lateef, Anjum Ayub, Saman Fatima, Huzifa Ahmed, Hassan Munir, Khalid Mohammed Khan

{"title":"A novel approach to urease inhibition and antioxidant therapeutics by using AgNPs-doped TiO2NPs stabilized by short chain pyrazole carbothioamide derivatives.","authors":"Rafia Usman Khan, Faiza Saleem, Mehreen Lateef, Anjum Ayub, Saman Fatima, Huzifa Ahmed, Hassan Munir, Khalid Mohammed Khan","doi":"10.36721/PJPS.2025.38.4.REG.13601.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.13601.1","url":null,"abstract":"This study details our approach to the synthesis, characterization and applications of silver nanoparticles (AgNPs) doped titanium dioxide nanoparticles (TiO2NPs). Prior to doping both the nanoparticles AgNPs and TiO2NPs were stabilized by a new small chain 5-(3-Nitrophenyl) Hydrazono-2-Pyridine-4,5-Dihydro-1H-Pyrazole-1-Thioamide (PDPC). The PDPC's small alkyl chain demonstrated efficient modulation over the growth dynamics and structural characteristics of AgNPs doped TiO2NPs system. An extensive study of the electronic and photonic properties of PDPC-stabilized (AgNPs) integrated into (TiO2NPs) was conducted utilizing advanced methods such as ultraviolet-visible absorption spectroscopy, atomic force microscope and fourier-transform IR. The atomic force microscopy (AFM) analysis revealed the morphology of the PDPC-stabilized (AgNPs) incorporated into (TiO2NPs), with an average diameter measured at 3±1 nm. The synthesized PDPC-stabilized (AgNPs) doped with (TiO2NPs) demonstrated significant antioxidant activity, achieving 82 ± 0.01% inhibition, alongside notable urease inhibition, with an efficacy of 80 ± 0.53% inhibition. Additionally, this composite system was effective in the reduction of Para-nitrophenol (PNP) and Methylene-blue (MB) dye. The integration of PDPC-stabilized AgNPs doped TiO2NPs facilitated the rapid transformation of p-nitrophenol and Methylene-blue into p-aminophenol (p-Amp) and leuco Methylene-blue (LMB) within one second, utilizing sodium borohydride (NaBH4) at normal temperature and pressure conditions demonstrated a kinetic behavior typical of pseudo-first-order reactions.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1484-1497"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive omics analysis of type 2 diabetes mellitus and cardioembolic stroke provides new biological insights and therapeutic targets. 2型糖尿病和心脏栓塞性中风的综合组学分析提供了新的生物学见解和治疗靶点。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.14341.1

Yue Hao, Jun-Tao Zhang, Li-Fen Guo, Heng-Qian He, Li-Qin Ying, Si-Yu Xian, Hao Liu, Qin-Kang Lu

{"title":"Comprehensive omics analysis of type 2 diabetes mellitus and cardioembolic stroke provides new biological insights and therapeutic targets.","authors":"Yue Hao, Jun-Tao Zhang, Li-Fen Guo, Heng-Qian He, Li-Qin Ying, Si-Yu Xian, Hao Liu, Qin-Kang Lu","doi":"10.36721/PJPS.2025.38.4.REG.14341.1","DOIUrl":"10.36721/PJPS.2025.38.4.REG.14341.1","url":null,"abstract":"The objective of this study is to investigate the shared genes that are differentially expressed (DEGs) between CES and T2DM, as well as uncover the hidden molecular mechanisms involved. We retrieved the gene expression profiles for CES (GSE58294) and T2DM (GSE25724) from Gene Expression Omnibus (GEO) database. We then per formed 5 analyses: Identify the overlapping DEGs between CES and T2DM, correlation analysis of hub genes; transcriptional regulation analysis of hub genes; single-cell sequencing analysis and potential therapeutic drug prediction. A total of 239 overlapping genes with the same trends were identified as DEGs between two datasets. Functional analysis emphasized the crucial role of neuronal cell development in these two diseases. Through the three algorithms of plug-in cytoHubba, five common hub genes were identified as HNRNPD, APP, ESR1, RHOA and DICER1. Single-cell analysis further confirmed the expression of five hub genes. In addition, TF (FOXC1) and miRNAs (miR-221-3p and miR-222-3p) were identified as potential key regulators between the CES and T2DM. This research reveals the shared pathogenesis of CES and T2DM. In the future, these common hub genes may provide new targets for further mechanistic research as well as new therapies for patients with CES and T2DM.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1448-1461"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the cytoprotective potential of oleuropein against indomethacin-induced gastric ulcer in rats. 橄榄苦苷对吲哚美辛致大鼠胃溃疡的细胞保护作用。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.12717.1

Yavas Adem, Sekkin Selim

{"title":"Exploring the cytoprotective potential of oleuropein against indomethacin-induced gastric ulcer in rats.","authors":"Yavas Adem, Sekkin Selim","doi":"10.36721/PJPS.2025.38.4.REG.12717.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.12717.1","url":null,"abstract":"Indomethacin (INDO) can induce gastric ulcers via oxidative stress and apoptosis. This study aimed to investigate the protective effects of oleuropein (OLE), a natural antioxidant from olive plants, against INDO-induced gastric ulcers in rats, focusing on the modulation of growth factors and apoptotic pathways. Forty-eight male Wistar rats were divided into six groups: control, INDO, lansoprazole (LAN), and OLE doses (6, 12, and 18 mg/kg). INDO was administered to induce ulcers, followed by respective OLE treatments. Analyses included comet assays for DNA damage, assessment of oxidative/antioxidative enzyme activities, qRT-PCR for growth factor expression, Western blots for apoptotic protein levels, and histopathological examination. INDO significantly increased ulcer area, malondialdehyde (MDA) levels, and apoptosis while reducing antioxidant enzymes and growth factor expression. OLE (18 mg/kg), significantly reduced ulcer area (P<0.001), increased activities of superoxide dismutase (SOD) and glutathione (GSH), decreased MDA levels (P<0.001) and protected lymphocytes from DNA damage. OLE also attenuated the INDO-induced downregulation of EGF, EGFR, VEGF, VEGFR1, and VEGFR2 (P<0.05). OLE counteracted the increase in Bax and caspase-3/9 and the reduction in Bcl-2 expression (P<0.05). OLE showed strong protection against INDO-induced ulcers by reducing oxidative stress, regulating growth factors, and inhibiting apoptosis, supporting its potential for treating gastric ulcers.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1296-1308"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the positive influence of Qianlie Shutong capsule combined with tamsulosin on prostate health of patients with prostatitis. 前列舒通胶囊联合坦索罗辛对前列腺炎患者前列腺健康的积极影响分析。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-07-01 DOI: 10.36721/PJPS.2025.38.4.REG.13500.1

Tao Cheng, Jing Ning, Dawen Ye, Zeyu Zha, Weiqiang Xu, Wenge Fang, Likai Mao, Mingli Gu

{"title":"Analysis of the positive influence of Qianlie Shutong capsule combined with tamsulosin on prostate health of patients with prostatitis.","authors":"Tao Cheng, Jing Ning, Dawen Ye, Zeyu Zha, Weiqiang Xu, Wenge Fang, Likai Mao, Mingli Gu","doi":"10.36721/PJPS.2025.38.4.REG.13500.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.4.REG.13500.1","url":null,"abstract":"Chronic prostatitis (CP) is one of the most common health issues in the male urinary system. In this study, we included 110 patients with CP as research subjects and divided them into a research group receiving Qianlie Shutong Capsule (QLSTC) combined with tamsulosin and a control group receiving tamsulosin monotherapy. Comparing their clinical efficacy, we found that the total effective rate of treatment in the research group was higher than that in the control group (P<0.05) and the clinical symptoms of CP were more significantly improved. Meanwhile, comparing the prostate function between the two groups, it was also seen that the small particle of lecithin (SBC) in the prostate fluid of the research group increased significantly after treatment (P<0.05). In addition, comparing the nutritional status of the two groups, we also found that the research group had better nutritional status after treatment. As for safety, there was no significant difference between the two groups (P>0.05). It can be seen that QLSTC combined with tamsulosin has extremely high clinical application value in the treatment of CP and is recommended to be used clinically.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 4","pages":"1328-1333"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0