Parasitology Research最新文献_第2页

Epidemiology of Strongyloides stercoralis and Opisthorchis viverrini infections in northern and northeastern Thailand: Insights from urine-ELISA surveys.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-23 DOI: 10.1007/s00436-024-08427-3

Wansiri Wiraphongthongchai, Paiboon Sithithaworn, Kavin Thinkhamrop, Kulwadee Suwannatrai, Kulthida Y Kopolrat, Chanika Worasith, Apiporn T Suwannatrai

{"title":"Epidemiology of Strongyloides stercoralis and Opisthorchis viverrini infections in northern and northeastern Thailand: Insights from urine-ELISA surveys.","authors":"Wansiri Wiraphongthongchai, Paiboon Sithithaworn, Kavin Thinkhamrop, Kulwadee Suwannatrai, Kulthida Y Kopolrat, Chanika Worasith, Apiporn T Suwannatrai","doi":"10.1007/s00436-024-08427-3","DOIUrl":"https://doi.org/10.1007/s00436-024-08427-3","url":null,"abstract":"Strongyloides stercoralis and Opisthorchis viverrini are helminth parasites responsible for significantly neglected tropical diseases. This study aimed to evaluate the prevalence of these parasites and the risk factors for S. stercoralis and O. viverrini infections in northern and northeastern Thailand where relevant epidemiological data are scarce and outdated. A cross-sectional study was conducted in 2016 in six sub-districts across five provinces. Urine samples were analyzed to detect S. stercoralis and O. viverrini infection using urine enzyme-linked immunosorbent assays (urine-ELISA). Demographic data of participants were collected using a questionnaire. Environmental data, including land-surface temperature (LST), normalized difference vegetation index (NDVI), normalized difference water index (NDWI), and soil pH, were obtained from remote-sensing sources. Multiple logistic regression analysis identified risk factors associated with infection. Complete data were obtained for 2613 individuals. The overall prevalence was 39.15% (95% CI: 37.27-41.02) for S. stercoralis and 37.46% (95% CI: 35.61-39.32) for O. viverrini. Male sex was significantly associated with S. stercoralis (AOR, 1.53; 95% CI, 1.29-1.80, p < 0.001) and O. viverrini infections (AOR, 1.69; 95% CI, 1.43-2.00, p < 0.001). The prevalence of S. stercoralis infection increased with age. Spatially, the odds of S. stercoralis infection decreased with higher LST and soil pH, while O. viverrini infection was associated with higher soil pH and proximity to water bodies. Strongyloides stercoralis and O. viverrini are highly prevalent in these regions, highlighting the need for surveillance.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"417"},"PeriodicalIF":1.8,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gasterophilus in horses from Romania: diversity, prevalence, seasonal dynamics, and distribution.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-23 DOI: 10.1007/s00436-024-08419-3

Ș O Rabei, A S Cârstolovean, C A Culda, A D Mihalca

{"title":"Gasterophilus in horses from Romania: diversity, prevalence, seasonal dynamics, and distribution.","authors":"Ș O Rabei, A S Cârstolovean, C A Culda, A D Mihalca","doi":"10.1007/s00436-024-08419-3","DOIUrl":"10.1007/s00436-024-08419-3","url":null,"abstract":"This is the first study that targets the epidemiology of Gasterophilus spp. in slaughtered horses from Romania. Previously to our research, there were five recorded species: Gasterophilus haemorrhoidalis, Gasterophilus inermis, Gasterophilus intestinalis, Gasterophilus nasalis, and Gasterophilus pecorum with a dispersed distribution throughout the country, the data being recorded more than 73 years ago. The collection of Gasterophilus larvae was carried out from the digestive system (stomach, duodenum, and rectum) of horses in three abattoirs, monthly between January 2023 and December 2023. Following dissection, all larvae were stored in absolute ethanol until morphological and molecular identification. The larvae were counted and identified according to morphological keys. Overall, 394 horses were examined, of which 211 (53.6%) were infected with L2 or L3 of Gasterophilus spp. Only two species were found: G. intestinalis (prevalence 52.0%) and G. nasalis (14.0%). Coinfection with both species occurred in 49 horses (12.4%). In total, 9759 larvae were collected (7916 L3 and 1843 L2). Larvae were localized in the stomach (G. intestinalis) and duodenum (G. nasalis) of horses. There was a strong seasonal variation in the mean intensity and mean abundance for both G. intestinalis and G. nasalis larvae and also for coinfections (p < 0.05). There were no significant differences between prevalence and mean intensity across gender, group age, or horse colour. Our findings show that Gasterophilus infection is widespread within Romania territory, being a possible cause of illness and welfare issues in horses.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"416"},"PeriodicalIF":1.8,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Echinococcus multilocularis delta/notch signalling components are expressed in post-mitotic cells.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-23 DOI: 10.1007/s00436-024-08442-4

Chris Speicher, Monika Bergmann, Klaus Brehm

{"title":"Echinococcus multilocularis delta/notch signalling components are expressed in post-mitotic cells.","authors":"Chris Speicher, Monika Bergmann, Klaus Brehm","doi":"10.1007/s00436-024-08442-4","DOIUrl":"https://doi.org/10.1007/s00436-024-08442-4","url":null,"abstract":"Pluripotent somatic stem cells are the drivers of unlimited growth of Echinococcus multilocularis metacestode tissue within the organs of the intermediate host. To understand the dynamics of parasite proliferation within the host, it is therefore important to delineate basic mechanisms of Echinococcus stem cell maintenance and differentiation. We herein undertake the first step towards characterizing the role of an evolutionarily old metazoan cell-cell communication system, delta/notch signalling, in Echinococcus cell fate decisions. Bioinformatic analyses revealed that all central components of this pathway are encoded by the Echinococcus genome and are expressed in parasite larval stages. By in situ hybridisation, we analyzed the expression patterns of clearly identified delta-like ligands, delta1 and delta2, as well as two notch receptors, notch1 and notch2, in metacestode tissue. Except for delta1, which is not expressed in the metacestode, all other components are expressed in distinct cells throughout the parasite's germinal layer. Combined in situ hybridisation and EdU incorporation experiments together with pulse-chase assays further indicate that delta2, notch1, and notch2 are exclusively expressed in post-mitotic cells. Echinococcus asymmetric stem cell division, leading to the progeny of different fates, therefore most probably not involves delta/notch signalling components. Our analyses are relevant for understanding the interplay of fate-determining signalling pathways in Echinococcus cell differentiation and form a basis for further experiments into the role of delta/notch signalling in parasite development.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"418"},"PeriodicalIF":1.8,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An epidemiological survey of bovine piroplasmosis in Kashgar, Xinjiang, China.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-20 DOI: 10.1007/s00436-024-08439-z

Yuxia Zhang, Xuke Chen, Yanyan Zhang, Na Pu, Wenqing Zhao, Zhengrong Wang, Yan Sun, Chunying Jia, Xinwen Bo

{"title":"An epidemiological survey of bovine piroplasmosis in Kashgar, Xinjiang, China.","authors":"Yuxia Zhang, Xuke Chen, Yanyan Zhang, Na Pu, Wenqing Zhao, Zhengrong Wang, Yan Sun, Chunying Jia, Xinwen Bo","doi":"10.1007/s00436-024-08439-z","DOIUrl":"https://doi.org/10.1007/s00436-024-08439-z","url":null,"abstract":"Piroplasmosis is an important tick-borne disease in several regions, and can lead to significant economic animal production losses. The current study aimed to systematically examine the incidence of bovine piroplasmosis in Kashgar, Xinjiang, to provide baseline data for the effective prevention and control of this disease among bovines in the region. A total of 1403 bovine blood samples from 12 sampling points were screened via PCR with universal Piroplasma primers targeting the 18S rRNA locus and specific Theileria annulata primers targeting the cytochrome b (COB) gene. The overall prevalence of bovine Piroplasma was 65.9% (925/1403). Three species of pathogenic Theileria, including T. annulata, T. orientalis, and T. sinensis, were detected, and the infection rates for these species were 65.1% (913/1403), 0.5% (7/1403), and 0.1% (1/1403), respectively. The mixed infection rate for T. orientalis and T. annulata was 0.3% (4/1403). No Babesia was detected in this study. In conclusion, bovine piroplasmosis was still common in Kashgar and T. annulata was the dominant species, and a mixed infection of T. annulata and T. orientalis was detected. Notably, T. sinensis was reported for the first time in this region. Therefore, strategies for the prevention and control of bovine piroplasmosis should be enhanced.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"415"},"PeriodicalIF":1.8,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk factors for Clonorchis sinensis infections and control measures reinforced with focused interviews.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-19 DOI: 10.1007/s00436-024-08437-1

Soon-Ok Lee, Fuhong Dai, Eun Joo Park, Yun Pyo Hong, Fu-Shi Quan, Jin-Ho Song, Sung-Jong Hong

{"title":"Risk factors for Clonorchis sinensis infections and control measures reinforced with focused interviews.","authors":"Soon-Ok Lee, Fuhong Dai, Eun Joo Park, Yun Pyo Hong, Fu-Shi Quan, Jin-Ho Song, Sung-Jong Hong","doi":"10.1007/s00436-024-08437-1","DOIUrl":"https://doi.org/10.1007/s00436-024-08437-1","url":null,"abstract":"Clonorchiosis, a disease that develops following the consumption of raw freshwater fish that harbors Clonorchis sinensis metacercariae, seriously threatens the health of residents in endemic areas. We aimed to assess the level of awareness of liver fluke infections among infected residents and restaurant workers in liver fluke infection-endemic areas. To identify and provide a more realistic description of C. sinensis infections, we conducted a focused interview and questionnaire survey of local residents in endemic areas in Korea. Most residents knew that freshwater fish are a source of C. sinensis infection. Nevertheless, the consumption of raw freshwater fish was the main risk factor for their infection with C. sinensis. The residents had little knowledge regarding their residential area being endemic to C. sinensis or about the symptoms and complications of C. sinensis infection. Although the participants had no experience with C. sinensis prevention or health education, they preferred on-site education during visits to village halls. The in-depth interviewees had a high level of knowledge about C. sinensis infection but were unaware that eating raw freshwater fish was its cause. Family and social gatherings encourage the habit of eating raw freshwater fish, which is a risk factor for repetitive infections. Based on these findings, to eliminate clonorchiosis, we propose strengthening the capacity of public health care personnel, giving C. sinensis prevention education, and screening educational videos during visits to village halls. Furthermore, intensive education is recommended for reinfected people and community opinion leaders, as well as reservoir host control.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"414"},"PeriodicalIF":1.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel ATP-binding cassette protein (NoboABCG1.3) plays a role in the proliferation of Nosema bombycis.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-19 DOI: 10.1007/s00436-024-08440-6

Shaogang He, Shiyi Zheng, Honglin Zhu, Yuanke Hu, Bin Yu, Junhong Wei, Guoqing Pan, Zeyang Zhou, Chunfeng Li

{"title":"A novel ATP-binding cassette protein (NoboABCG1.3) plays a role in the proliferation of Nosema bombycis.","authors":"Shaogang He, Shiyi Zheng, Honglin Zhu, Yuanke Hu, Bin Yu, Junhong Wei, Guoqing Pan, Zeyang Zhou, Chunfeng Li","doi":"10.1007/s00436-024-08440-6","DOIUrl":"https://doi.org/10.1007/s00436-024-08440-6","url":null,"abstract":"ATP-binding cassette (ABC) transporter proteins, one of the largest families of membrane transport proteins, participate in almost all biological processes and widely exist in living organisms. Microsporidia are intracellular parasites; they can reduce crop yields and pose a threat to human health. The ABC proteins are also present in microsporidia and play a critical role in their proliferation and energy transport. In this study, a novel ABC transporter protein of Nosema bombycis named NoboABCG1.3 was identified. The NoboABCG1.3 protein is comprised of 640 amino acids, which contain six transmembrane domains and one nucleotide-binding domain. After N. bombycis infection of cells or tissues, quantitative reverse transcription polymerase chain reaction analysis revealed a progressive elevation in the transcript levels of NoboABCG1.3. Downregulation of NoboABCG1.3 expression significantly inhibited N. bombycis proliferation. Subsequently, a transgenic cell line stably expressing an interfering fragment of NoboABCG1.3 was established, which exhibited extreme inhibition on the proliferation of N. bombycis. These findings indicate that NoboABCG1.3 plays a role in the proliferation of N. bombycis and holds promise as a target for developing N. bombycis-resistant silkworms.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"413"},"PeriodicalIF":1.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of Dirofilaria immitis in dogs in Jamaica.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-18 DOI: 10.1007/s00436-024-08434-4

Nadia K Khouri, Satish Singh, Simmoy A A Noble, Tiffany V Kirlew, Mario A J Golding, Inshan Ali, Cameil Wilson-Clarke, Paul Cadogan, John F Lindo, Simone L Sandiford

{"title":"Prevalence of Dirofilaria immitis in dogs in Jamaica.","authors":"Nadia K Khouri, Satish Singh, Simmoy A A Noble, Tiffany V Kirlew, Mario A J Golding, Inshan Ali, Cameil Wilson-Clarke, Paul Cadogan, John F Lindo, Simone L Sandiford","doi":"10.1007/s00436-024-08434-4","DOIUrl":"https://doi.org/10.1007/s00436-024-08434-4","url":null,"abstract":"Dirofilaria immitis is a zoonotic vector-borne parasite that causes heartworm disease most commonly in canines. Although present in the Caribbean, no published work exists from Jamaica. Therefore, the aim of our study was to evaluate the prevalence of D. immitis in Jamaica. From June to December 2022, whole blood samples from 1000 dogs were collected from all parishes and tested using the Bionote Canine Heartworm Antigen Test Kit 2.0. Dogs that were at least 6 months old, privately owned, residing in shelters, working in the security sector, or strays were recruited into the study. Details on the breed, known or approximate age, sex, use of heartworm prophylaxis, and other medications and location of animals were provided by owners/authorizing agents and recorded. Test results were available for 986 dogs of which 184 tested positive, with an overall prevalence of 18.7% (95% CI, 16.28 to 21.24). Mature adults (2-6 years) comprised most of our sampled population (46.7%), followed by early seniors (7-9 years) (20.1%) and young adults (13-24 months) (9.9%). Prevalence for those age groups was 21.9%, 19.5%, and 7.1%, respectively. A seropositivity of 25.1% was seen in dogs not on heartworm preventative medication compared to 5.5% of dogs on heartworm preventative medication. Our study highlights a higher seroprevalence of D. immitis infections in older dogs and provides evidence supporting the use of preventive medication in the country.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"411"},"PeriodicalIF":1.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of 'soft flesh' condition induced by Kudoa thyrsites parasite in the Iberian European sardine stock. 伊比利亚欧洲沙丁鱼种群中 Kudoa thyrsites 寄生虫诱发的 "软肉 "病症。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-18 DOI: 10.1007/s00436-024-08436-2

Lucilla Giulietti, Gema Hernandez-Milian, Paolo Cipriani, Miguel Bao, Hui-Shan Tung, Carmen Hernández, Arne Levsen

{"title":"Occurrence of 'soft flesh' condition induced by Kudoa thyrsites parasite in the Iberian European sardine stock.","authors":"Lucilla Giulietti, Gema Hernandez-Milian, Paolo Cipriani, Miguel Bao, Hui-Shan Tung, Carmen Hernández, Arne Levsen","doi":"10.1007/s00436-024-08436-2","DOIUrl":"10.1007/s00436-024-08436-2","url":null,"abstract":"European sardine Sardina pilchardus is a commercially valuable coastal pelagic fish species. Spain is one of the largest sardine suppliers in Europe and the Iberian stock is of particular significance. Kudoa parasites are known to infect sardines causing the so-called 'soft flesh' condition; however, data on the occurrence of 'soft flesh' in this sardine stock are limited. This study investigates the occurrence of Kudoa-induced 'soft flesh' in the Iberian sardine stock caught in 2023 off the northern Spanish Atlantic coast (Division 8.c). Five hundred specimens were examined for 'soft flesh' by manual texture testing and visual inspection 48 h post-catch using standardized procedures. 'Soft flesh' was detected in 5.4% (27/500) of the sardines. Microscopic examination of muscle samples revealed the presence of Kudoa thyrsites-like myxospores in all 'soft flesh'-affected fish, which based on SSU rDNA gene sequence analysis was identified as K. thyrsites. The unsightly appearance of infected fillets represents a substantial food quality issue for the Iberian sardine stock that could reduce marketability and consumer confidence in both local and international markets. This is particularly relevant since larger Iberian sardines, which are highly appreciated by consumers, appear to be the most affected.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"412"},"PeriodicalIF":1.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and expression analysis of heat shock proteins HfHspc1 and HfHspc4 in Haemaphysalis flava ticks.

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-17 DOI: 10.1007/s00436-024-08422-8

Yong Li, Lan-Lan Wang, Li-Li Feng, De-Yong Duan, Darko Mihaljica, Tian-Yin Cheng

引用次数: 0

Prevalence, risk factors, and species diversity of strongylid nematodes in domesticated Thai horses: insights from ITS-2 rDNA metabarcoding. 泰国驯养马强直性线虫的流行率、风险因素和物种多样性：ITS-2 rDNA 代谢编码的启示。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-12-17 DOI: 10.1007/s00436-024-08438-0

Mohamed H Hamad, Wanarit Jitsamai, Teerapol Chinkangsadarn, Tasorn Sa Ngangam, Tanakorn Wattanapornpilom, Darm Naraporn, Suraseha Ouisuwan, Piyanan Taweethavonsawat

{"title":"Prevalence, risk factors, and species diversity of strongylid nematodes in domesticated Thai horses: insights from ITS-2 rDNA metabarcoding.","authors":"Mohamed H Hamad, Wanarit Jitsamai, Teerapol Chinkangsadarn, Tasorn Sa Ngangam, Tanakorn Wattanapornpilom, Darm Naraporn, Suraseha Ouisuwan, Piyanan Taweethavonsawat","doi":"10.1007/s00436-024-08438-0","DOIUrl":"10.1007/s00436-024-08438-0","url":null,"abstract":"Strongylid nematodes represent a major health and performance concern for equids globally. However, the epidemiology of strongylid infections in horse populations remains largely unexplored in Thailand. This study investigated the prevalence of strongylid parasites and the associated risk factors in domesticated horses in Thailand. Additionally, the study utilized ITS-2 rDNA metabarcoding to characterize the diversity and co-occurrence patterns of strongylid species. Of the 408 horses examined, 50.98% tested positive for strongyle infection, with an average intensity of 445.67 ± 639.58 eggs. Notably, only 25.74% exhibited fecal egg counts of ≥ 200 eggs per gram (EPG), highlighting the need for targeted deworming protocols. Significantly higher EPG values were observed in yearling horses (p = 0.001) and those kept in outdoor pastures (p = 0.0001). Metabarcoding identified 15 strongylid species, with Cylicostephanus longibursatus being the most abundant (mean relative abundance: 37.30%, SD = 31.16%). No Strongylus species were detected. Alpha diversity analysis revealed no significant differences in species richness and evenness across horse groups, while beta diversity analysis showed significant dissimilarities (p = 0.004), primarily driven by Cylicostephanus longibursatus, Cyathostomum pateratum, and Cylicostephanus calicatus, which contributed to over 60% of the variation. Species co-occurrence patterns were largely random, with a limited number of positive (n = 5) and negative (n = 2) species pair associations. These findings provide essential insights into the current state of strongylid infections in Thai horses and offer a foundation for future research and management strategies.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 12","pages":"410"},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0