Parasitology Research最新文献

Chewing lice (Phthiraptera) on a wild Golden eagle (Aquila chrysaetos) and a zoo-kept Eurasian griffon vulture (Gyps fulvus) in Tyrol, Austria. 在奥地利蒂罗尔，一只野生金雕（金雕）和一只动物园饲养的欧亚狮鹫（金雕）咀嚼着虱子（翅翅目）。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-21 DOI: 10.1007/s00436-025-08531-y

Jutta Pikalo, Oldrich Sychra, Miguel Peña-Espinoza, Maryna Galat, Maria Sophia Unterköfler, Mike Heddergott, Walter Glawischnig, Hans-Peter Fuehrer

引用次数: 0

Discovery of new chromosomal markers through repeatome analysis of Caryophyllaeus laticeps (Caryophyllidea). 通过对石竹科石竹重复组分析发现新的染色体标记。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-18 DOI: 10.1007/s00436-025-08530-z

Anna Marková, Martina Orosová

{"title":"Discovery of new chromosomal markers through repeatome analysis of Caryophyllaeus laticeps (Caryophyllidea).","authors":"Anna Marková, Martina Orosová","doi":"10.1007/s00436-025-08530-z","DOIUrl":"10.1007/s00436-025-08530-z","url":null,"abstract":"Genomic repetitive elements are essential structural and functional components of most eukaryotes and have attracted the attention of researchers in recent years. Despite the well-documented features of the karyotype of the tapeworm species Caryophyllaeus laticeps, knowledge about the content and distribution of repetitive elements on its chromosomes is limited. To gain a more comprehensive understanding of the composition of the repetitive DNA, we used a combination of bioinformatic, cytogenetic and molecular techniques to characterise the repetitive DNA content of this species. RepeatExplorer2 analysis revealed that transposable elements are predominantly present in the C. laticeps genome. The most suitable repeat families were selected for fluorescence mapping on the chromosomes, and most of them showed a scattered hybridisation pattern. In this study, we identified three new chromosomal markers: small nuclear U1 DNA (snDNA), the 5S ribosomal DNA (5S rDNA) and a satellite DNA named ClatSat14-167, each of which hybridises to a single locus at different chromosomal locations, allowing accurate identification of three pairs of chromosomes. These markers could be very valuable tools for future evolutionary studies and greatly enrich the limited cytogenetic data for tapeworms. In addition, an increased frequency of chromosomal aberrations was detected, reaffirming previous findings on the possible adverse effects of environmental pollution on the chromosomes of the species studied.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"84"},"PeriodicalIF":1.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12274263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trypanosoma cruzi: A new system for primary culture and isolation of the parasite. 克氏锥虫：一种新的原代培养和分离系统。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-16 DOI: 10.1007/s00436-025-08511-2

María Alejandra Vanegas, Laura Vanessa Téllez, Orlando Alfredo Torres, Paula Ximena Pavía

{"title":"Trypanosoma cruzi: A new system for primary culture and isolation of the parasite.","authors":"María Alejandra Vanegas, Laura Vanessa Téllez, Orlando Alfredo Torres, Paula Ximena Pavía","doi":"10.1007/s00436-025-08511-2","DOIUrl":"10.1007/s00436-025-08511-2","url":null,"abstract":"Various methods, including blood culture, are employed to isolate Trypanosoma cruzi. However, there is currently no standardized protocol for parasite culture, and the effectiveness of available techniques varies. In this study, we developed a standardized closed blood culture system (CBCS) designed to support the survival of trypomastigotes and their differentiation into epimastigotes, from sample collection through to primary laboratory isolation. Blood samples were artificially infected with varying concentrations of T. cruzi trypomastigotes to assess the performance of the CBCS. The CBCS enabled successful isolation and exponential growth of the parasite, demonstrating performance comparable to that of conventional culture methods. All assays included a reference control culture, which served as a benchmark for comparison. No contamination events were observed, and it was possible to isolate and expand the parasite population from an initial sample containing as few as ten trypomastigotes. The standardized CBCS protocol demonstrated good precision, as confirmed by repeatability and reproducibility tests, which showed acceptable variability among replicates.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"83"},"PeriodicalIF":1.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144643159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cattle tick-borne diseases: study of knowledge and practices among communal farmers in selected areas of the Limpopo Province, South Africa. 牛蜱传疾病：对南非林波波省选定地区社区农民的知识和做法的研究

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-15 DOI: 10.1007/s00436-025-08529-6

Realeboga Masego Gaorekwe, Maphuti Betty Ledwaba, Rae Marvin Smith, Dikeledi Petunia Malatji

{"title":"Cattle tick-borne diseases: study of knowledge and practices among communal farmers in selected areas of the Limpopo Province, South Africa.","authors":"Realeboga Masego Gaorekwe, Maphuti Betty Ledwaba, Rae Marvin Smith, Dikeledi Petunia Malatji","doi":"10.1007/s00436-025-08529-6","DOIUrl":"10.1007/s00436-025-08529-6","url":null,"abstract":"Tick-borne diseases (TBDs) pose significant challenges to livestock farmers, particularly in subtropical and tropical regions. This study aimed to assess the knowledge and practices of community cattle farmers on TBDs in Bela-Bela, Limpopo Province, South Africa. A semi-structured questionnaire was administered to 50 farmers, focused on socio-demographic information, TBD knowledge, and livestock management methods. The study found that while 78% of farmers were aware of heartwater and redwater, only 60% had sufficient knowledge to treat these diseases successfully. Terramycin and Swamycin (oxytetracycline hydrochloride) were the most used treatments, although most farmers were unaware of appropriate treatment methods for redwater. In addition to these conventional treatments, some farmers employed Ultravec as an alternative therapeutic option to manage redwater in their livestock. There was a lack of knowledge about tick species and effective management practices, and most farmers did not implement biosecurity measures to regulate livestock movements or reduce the spread of infection. The survey found that most farmers practiced extensive farming and grazed livestock mainly on communal land. Seasonal variations in disease outbreaks have been observed, highlighting the need for improved education and training on TBD management, biosecurity measures, and tick control approaches. The results indicate a significant (P ≤ 0.05) association between gender and the number of cattle reared. However, there was no significant association between farmers' demographics and knowledge of TBDs. Strengthening farmers' understanding of disease prevention and improving access to veterinary services are critical to reducing the economic burden of TBDs in community farming systems. In addition, the use of indigenous cattle breeds with inherent tick and disease resistance is vital.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"82"},"PeriodicalIF":1.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decomposition of functional beta, but not alpha, diversity detects deviations from the "host-diversity-begets-parasite-diversity" rule in flea-mammal associations. 分解功能性的多样性，而不是功能性的多样性，可以发现跳蚤和哺乳动物之间“宿主-多样性-繁殖-寄生虫-多样性”规律的偏离。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-08 DOI: 10.1007/s00436-025-08527-8

Boris R Krasnov, Irina S Khokhlova

{"title":"Decomposition of functional beta, but not alpha, diversity detects deviations from the \"host-diversity-begets-parasite-diversity\" rule in flea-mammal associations.","authors":"Boris R Krasnov, Irina S Khokhlova","doi":"10.1007/s00436-025-08527-8","DOIUrl":"10.1007/s00436-025-08527-8","url":null,"abstract":"The \"host-diversity-begets-parasite-diversity\" (HDBPD) pattern has been demonstrated for traditional metrics of functional diversity. We applied the decomposition of functional alpha (functional diversity structure; FDS) and beta (functional resemblance; FR) diversity into (a) Simpson's dominance, functional redundancy, and functional diversity and (b) taxonomic similarity, functional dissimilarity, and beta redundancy, respectively, coupled with ternary diagrams, to test whether the HDBPD rule also holds for FDS and FR and their separate components for communities of fleas and small mammals from different biomes of the Palearctic. We compared patterns of FDS and FR and their components within and between fleas and hosts within and between biomes and tested for correlation of separate components of functional alpha and beta diversities between fleas and hosts. Differences in FR between biomes were detected for both fleas and hosts (due to differences in each FR component), but not in FDS. Within biomes, fleas and hosts demonstrated marginally significant differences in FDS in steppes and temperate forests due to differences in the functional diversity component. Differences in FR between fleas and hosts were found in four of five biomes (due to differences in one or two FR components). Values of each of the three separate components of the flea FDS correlated positively with values of the respective components of the host FDS suggesting the effect of functional alpha diversity of hosts on that of fleas. No correlation between separate components of the flea and host FR was found implying no link between host and flea functional beta diversity. We conclude that the HDBPD rule, when applied to functional beta diversity, is not as universal as it was previously thought to be.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"80"},"PeriodicalIF":1.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterobius vermicularis infection and its risk factors among rural schoolchildren in Zhejiang Province: a cross-sectional study. 浙江省农村学龄儿童蛲虫感染及其危险因素的横断面研究

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-08 DOI: 10.1007/s00436-025-08525-w

Jialie Jin, Wei Ruan, Wenjie Xu, Xuan Zhang, Kegen Yu, Hualiang Chen, Jiaqi Zhang, Jimin Sun, Jianmin Jiang, Xiaoxiao Wang

{"title":"Enterobius vermicularis infection and its risk factors among rural schoolchildren in Zhejiang Province: a cross-sectional study.","authors":"Jialie Jin, Wei Ruan, Wenjie Xu, Xuan Zhang, Kegen Yu, Hualiang Chen, Jiaqi Zhang, Jimin Sun, Jianmin Jiang, Xiaoxiao Wang","doi":"10.1007/s00436-025-08525-w","DOIUrl":"10.1007/s00436-025-08525-w","url":null,"abstract":"Pinworm infection, caused by Enterobius vermicularis, is highly prevalent in humans, with children being particularly susceptible. This cross-sectional study (2022-2024) investigated the epidemiology and risk factors of E. vermicularis infection among 1179 rural schoolchildren (3-9 years) across four districts in Zhejiang Province. In each district, two kindergartens and two primary schools were selected using simple random sampling. Pinworm infection was identified using adhesive cellophane tape perianal swabbing method. Data on demographics, hygiene habits, and school environment were collected through structured questionnaires. Simple logistic regression analysis was performed for each potential risk factor, and those variables with a P value less than 0.1 were selected for the multivariate logistic regression analysis. The overall infection rate was 4.07%, with the highest prevalence in Xinchang (9.79%), followed by Daishan (2.68%), Wucheng (2.44%), and Yongkang (1.64%). The positive rate among girls was higher than that of boys (5.41% vs. 2.81%, P = 0.020). Among girls, older subjects had a notably higher infection rate compared to younger ones (8.08% vs. 3.19%). Besides, the infection rate of primary school subjects was higher than that of kindergarten subjects (7.48% vs. 3.23%). No significant differences were found in the boys' group. Furthermore, weekly classroom disinfection and monthly health education were associated with lower infection rates. This study underscores the prevalence of E. vermicularis infection in rural schoolchildren in Zhejiang Province and identifies significant risk factors. Strengthening hygiene education and sanitation practices, particularly targeting female students, may serve as effective measures against E. vermicularis infection.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"81"},"PeriodicalIF":1.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimization of an ELISA method to detect Toxoplasma gondii oocyst infection in cats. ELISA检测猫刚地弓形虫卵囊感染方法的建立与优化。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-07 DOI: 10.1007/s00436-025-08523-y

Mingfeng He, Bufan Zhang, Shuai Han, Jiahui Qian, Zhengming He, Yulian Wei, Yanqin Zhou, Bang Shen, Rui Fang

{"title":"Development and optimization of an ELISA method to detect Toxoplasma gondii oocyst infection in cats.","authors":"Mingfeng He, Bufan Zhang, Shuai Han, Jiahui Qian, Zhengming He, Yulian Wei, Yanqin Zhou, Bang Shen, Rui Fang","doi":"10.1007/s00436-025-08523-y","DOIUrl":"10.1007/s00436-025-08523-y","url":null,"abstract":"Toxoplasmosis caused by Toxoplasma gondii (T. gondii) is a zoonotic disease with great medical and veterinary significance. Felines, the definitive hosts of T. gondii, play a crucial role in the transmission of toxoplasmosis. The booming pet industry has led to more cats and cat-owning families, increasing the risk of toxoplasmosis transmission from animals to humans. Monitoring feline T. gondii infection accurately is crucial for reducing transmission risks. However, existing diagnostic methods focus on detecting whether cats are infected with T. gondii but fail to trace whether feline toxoplasmosis infections originate from oocysts or cysts. In this study, we assessed four late-stage development abundant proteins that were highly expressed specifically in sporulated oocysts to evaluate their specificity in binding to cat anti-T. gondii-oocyst serum. The LEA880 protein can only specifically react with cat anti-T. gondii-oocyst positive serum, but not with cat anti-T. gondii-cyst positive serum or negative cat serum. The optimized indirect enzyme-linked immunosorbent assay (iELISA) method based on the LEA880 protein exhibits good specificity and sensitivity in detecting T. gondii oocyst infection in cats.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"79"},"PeriodicalIF":1.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical characterization and bioactivity of cigarette butt extract as oviposition deterrent and larvicide against Aedes aegypti. 烟蒂提取物对埃及伊蚊的阻卵杀幼虫化学特性及生物活性研究。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-05 DOI: 10.1007/s00436-025-08502-3

Rana Zain Khizar, Farrukh Baig, Unsar Naeem-Ullah, Naeem Iqbal, Abid Hussain

{"title":"Chemical characterization and bioactivity of cigarette butt extract as oviposition deterrent and larvicide against Aedes aegypti.","authors":"Rana Zain Khizar, Farrukh Baig, Unsar Naeem-Ullah, Naeem Iqbal, Abid Hussain","doi":"10.1007/s00436-025-08502-3","DOIUrl":"10.1007/s00436-025-08502-3","url":null,"abstract":"Aedes aegypti, the primary vector of viruses such as dengue, chikungunya, and yellow fever, poses a global health challenge. Traditional vector control strategies predominantly rely on broad-spectrum insecticides, which not only contribute to development of resistance in mosquitoes but also pose significant risks to non-target organisms and the environment. Here, we assessed the potential of cigarette butt (CB) extract for the management of A. aegypti population. Globally, 4.5 trillion CBs are being wasted annually, which is expected to rise to nine trillion by 2025. So, repurposing this waste for vector control offers an innovative approach. This study evaluated the potential of CB extract to deter ovipositing females and to assess larval mortality of A. aegypti using three concentrations of CB extract (1 CB, 2 CBs, and 3 CBs) against a control (water only). Our results revealed that highest concentration (3 CBs) significantly repelled the egg-laying females and resulted in 100% mortality of younger mosquito larvae (1st and 2nd instars) within 24 h of exposure, whereas complete mortality in mature larvae (3rd and 4th instars) was observed within 48 h of exposure. GC-MS analysis of CB extracts, using acetone and methanol as solvents, revealed both qualitative and quantitative differences in the chemical composition. Identified compounds include nicotine, phthalic acid, terephthalic acid, and acetonyl dimethyl carbinol. This study presents an innovative solution for recycling cigarette butt waste to control A. aegypti population at both adult and larval stages and highlights the potential to utilize CB waste into integrated vector management programs.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"78"},"PeriodicalIF":1.8,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12228669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a qPCR molecular diagnostic assay for the detection of kiwi Eimeria species and its application to determine tissue-specificity of species causing coccidiosis in North Island brown kiwi (Apteryx mantelli). 建立一种检测猕猴桃艾美球虫的qPCR分子诊断方法及其在确定北岛褐猕猴桃球虫病病原菌组织特异性中的应用。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-04 DOI: 10.1007/s00436-025-08521-0

Emma Scheltema, Kerri Morgan, Stuart Hunter, John Mackay, Preet Singh, Laryssa Howe

{"title":"Development of a qPCR molecular diagnostic assay for the detection of kiwi Eimeria species and its application to determine tissue-specificity of species causing coccidiosis in North Island brown kiwi (Apteryx mantelli).","authors":"Emma Scheltema, Kerri Morgan, Stuart Hunter, John Mackay, Preet Singh, Laryssa Howe","doi":"10.1007/s00436-025-08521-0","DOIUrl":"10.1007/s00436-025-08521-0","url":null,"abstract":"Juvenile kiwi (Apteryx spp.) within captive-rearing programmes commonly suffer from coccidiosis, which primarily affects the intestine but can also impact other organs, such as the kidneys, liver, lung, and spleen (Morgan et al. Avian Pathol 42:137-146 2013). In some immune-compromised birds, disease causes significant morbidity and, occasionally, mortality (Morgan et al. NZVJ 62:315-320 2014); however, understanding of the biology of disease-causing Eimeria species in kiwi is limited. A probe-based qPCR assay targeting a 115-bp fragment of the Eimeria mitochondrial cytochrome c oxidase I (CO1) gene was developed to identify three distinct kiwi Eimeria species: the two species most commonly recovered from faeces, Eimeria kiwii and Eimeria apteryxii, as well as the newly described species, Eimeria koka (Scheltema et al. Syst Parasitol 102:30 2025). The qPCR assay was then applied to retrospectively analyse formalin-fixed paraffin-embedded intestine, kidney, liver, lung, and spleen tissues from ten historic post-mortem cases from kiwi diagnosed with extraintestinal coccidiosis. This novel assay detected infection more often (33/47 tissues) than manual histopathological identification (25/47 tissues). Only one species, E. koka, was detected in extraintestinal tissues with the highest prevalence (9/10) in kidney tissues. In contrast, E. kiwii was reliably detected in 8/9 intestinal tissues but was not detected in the other tissues tested. E. apteryxii was not detected in any of the tissues analysed. These findings suggest that kiwi are infected by at least one intestinal and one renal-specific species, the latter of which is suspected to disseminate under certain conditions to other organs of the body.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"77"},"PeriodicalIF":1.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtetrameres cloacitectus in Eurasian buzzard (Buteo buteo): pathology, phylogenetics, and seasonality. 欧亚秃鹫（Buteo Buteo）的阴囊小四足虫：病理、系统发育和季节性。

IF 1.8 3区医学

Parasitology Research Pub Date : 2025-07-02 DOI: 10.1007/s00436-025-08517-w

Katarzyna Anna Hołówka, Andrada Negoescu, Marian Taulescu, Angela Monica Ionică, Georgiana Deak, Andrei Daniel Mihalca, Călin Mircea Gherman

{"title":"Microtetrameres cloacitectus in Eurasian buzzard (Buteo buteo): pathology, phylogenetics, and seasonality.","authors":"Katarzyna Anna Hołówka, Andrada Negoescu, Marian Taulescu, Angela Monica Ionică, Georgiana Deak, Andrei Daniel Mihalca, Călin Mircea Gherman","doi":"10.1007/s00436-025-08517-w","DOIUrl":"10.1007/s00436-025-08517-w","url":null,"abstract":"Microtetrameres cloacitectus is a nematode of the family Tetrameridae Travassos, 1914, parasitising the proventricular glands of diurnal raptors. First described by Oschmarin (1956) from the Eurasian buzzard (Buteo buteo) in Primorsky Krai, Russia, it has since been reported in several raptor species. Between 2017 and 2024, 88 deceased Eurasian buzzards were collected from various regions in Romania. For each specimen, GPS coordinates, sex, and age were recorded, and full parasitological necropsies were performed. One individual (over three-year-old male found as roadkill in Tureni, Cluj County, Northwestern Romania) had Microtetrameres-like nematodes; a section of its proventriculus was preserved in 10% formalin for histopathological examination. In the remaining positive cases, nematodes were preserved in ethanol for molecular analyses and in 4% formalin for morphological identification. Overall, 25 buzzards were infected with M. cloacitectus, resulting in a prevalence of 28.4% (95% CI 20.0-38.6%). A total of 779 specimens (464♀, 315♂) were recovered, with an intensity range of 1-316 parasites (mean = 31.2). Infections showed a seasonal pattern, peaking in summer, and were more frequent in birds over 2 years old. Molecular analysis yielded a novel cox1 sequence and revealed new phylogenetic relationships. Histopathology revealed multifocal glandular distention and inflammatory nodules centred on parasites. This study provides the first comprehensive parasitological, molecular, and histopathological characterisation of M. cloacitectus in Romania.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 7","pages":"74"},"PeriodicalIF":1.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0