Parasitology Research最新文献

Recessivity of deltamethrin resistance, kdr mutations, and detoxifying enzymes in hybrids of Triatoma phyllosoma subcomplex from Mexico. 墨西哥 Triatoma phyllosoma 亚复合体杂交种中溴氰菊酯抗性、kdr 突变和解毒酶的递归性。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-28 DOI: 10.1007/s00436-024-08396-7

Jesus A Davila-Barboza, Selene M Gutierrez-Rodriguez, Beatriz Lopez-Monroy, Ildefonso Fernandez-Salas, Iram P Rodriguez-Sanchez, Alejandro Villegas-Trejo, Adriana E Flores

{"title":"Recessivity of deltamethrin resistance, kdr mutations, and detoxifying enzymes in hybrids of Triatoma phyllosoma subcomplex from Mexico.","authors":"Jesus A Davila-Barboza, Selene M Gutierrez-Rodriguez, Beatriz Lopez-Monroy, Ildefonso Fernandez-Salas, Iram P Rodriguez-Sanchez, Alejandro Villegas-Trejo, Adriana E Flores","doi":"10.1007/s00436-024-08396-7","DOIUrl":"https://doi.org/10.1007/s00436-024-08396-7","url":null,"abstract":"Triatoma species from the phyllosoma subcomplex are sympatrically distributed and include some of the main vectors of Chagas disease in Mexico. Species within this subcomplex, including Triatoma pallidipennis, T. mazzottii, T. picturata, and T. longipennis, have shown resistance to pyrethroid insecticides, associated with mutations in the para gene of the voltage gate sodium channel (VGSC) and the activity of detoxifying enzymes such as β-esterases and glutathione s-transferases (GST). In this study, we evaluated resistance to deltamethrin in hybrids of T. pallidipennis × T. mazzottii (T.pal × T.maz) and T. pallidipennis × T. picturata (T.pal × T.pic) under laboratory conditions, and the inheritance was determined based on the degree of dominance (DO). Additionally, associated resistance mechanisms were analyzed, including detoxifying enzymes and knockdown resistance (kdr) mutations. High levels of resistance to deltamethrin were found in the hybrids of T.pal × T.maz when compared with the susceptible strain of T. mazzottii (RR50 = 17.50). Dominance levels calculated for each hybrid showed values < - 1, confirming that resistance to deltamethrin was recessive. Hybrids exhibited reduced α-, β-esterases, and cytochrome P450 mixed-function oxidases (MFO) activity. However, both hybrids showed significantly increased GST activity, particularly in T.pal × T.pic, suggesting enhanced detoxification through this pathway. The kdr mutation A943V, present in T. mazzottii, was found in T.pal × T.maz hybrids. These results emphasize the importance of considering hybridization in resistance management programs and its potential impact on the success of insecticide-based control measures.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"399"},"PeriodicalIF":1.8,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory role of Trichinella spiralis-derived antigen on imiquimod-induced psoriasis in mice model. 螺旋毛霉衍生抗原对咪喹莫特诱导的小鼠银屑病模型的免疫调节作用

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-27 DOI: 10.1007/s00436-024-08415-7

Nahla El Skhawy, Maha M Eissa, Maram Allam, Eman M Eleryan

{"title":"Immunomodulatory role of Trichinella spiralis-derived antigen on imiquimod-induced psoriasis in mice model.","authors":"Nahla El Skhawy, Maha M Eissa, Maram Allam, Eman M Eleryan","doi":"10.1007/s00436-024-08415-7","DOIUrl":"https://doi.org/10.1007/s00436-024-08415-7","url":null,"abstract":"The immunomodulatory activity of parasites has been extensively investigated in multiple immune-related diseases. However, dermatological diseases have been off the list for a long time despite their vast incidence and the deleterious consequences of some of them. This study explored the immunomodulatory role of autoclaved Trichinella spiralis (T. spiralis) larvae antigen (ATSLA) as a psoriasis immunotherapeutic candidate in a mice model. Psoriasis was induced in Swiss albino mice using commercial imiquimod cream (IMQ). Mice were randomly divided into the IMQ untreated control group and the IMQ treated group that was treated with ATSLA twice, on day 0 and day 3. Additional mice served as normal controls. Assessment of skin thickness, erythema, and scales was recorded. Total skin scores were calculated. Skin MDA levels, splenic indices, serum and skin IL-23, and tumor necrosis factor alpha (TNF-α) were measured. Skin sections were stained with H&E and immune stained for CD68-positive cells using immunohistochemistry. Treatment with ATSLA significantly reduced skin thickness, erythema, scales, and total skin scores in the IMQ-treated group compared to the untreated control. This was accompanied by a reduction in the splenic index, skin MDA levels, IL-23, and TNF-α in both the skin and serum of the treated group. Pathologically, skin sections of the treated group showed less epidermal thickness, acanthosis, hyperkeratosis, and CD68 cell count. The study concluded the immunotherapeutic activity of ATSLA in experimental psoriatic skin lesions. This will enrich the psoriasis immunotherapeutic list with novel candidates of parasitic origin.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"397"},"PeriodicalIF":1.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142731808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Immunological Characterization of Annexin B8 and Annexin E1 from Spirometra Erinaceieuropaei Spargana. 螺旋藻中的 Annexin B8 和 Annexin E1 的鉴定和免疫学特征。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-27 DOI: 10.1007/s00436-024-08407-7

Ruijia Fu, Yanquan Xu, Gang Lu, Fan Zhang, Pei Liang, Dayong Wang

{"title":"Identification and Immunological Characterization of Annexin B8 and Annexin E1 from Spirometra Erinaceieuropaei Spargana.","authors":"Ruijia Fu, Yanquan Xu, Gang Lu, Fan Zhang, Pei Liang, Dayong Wang","doi":"10.1007/s00436-024-08407-7","DOIUrl":"https://doi.org/10.1007/s00436-024-08407-7","url":null,"abstract":"Sparganosis is a parasitic zoonotic disease that poses a serious threat to public hygiene and human health. Annexin is a phospholipid-binding protein with calcium ion binding activity, serving various important functions, including interaction with the parasite and regulation of the host's immune response. In this study, two annexin (ANX) family genes, Spirometra erinaceieuropaei (S. erinaceieuropaei) Annexin B8 (SeANXB8) and E1 (SeANXE1), isolated from spargana, were cloned and immunologically characterized. Both recombinant S. erinaceieuropaei Annexin B8 (rSeANXB8) and E1 (rSeANXE1) were specifically recognized by serum from rats immunized with the recombinant proteins, displaying strong immunoreactivity. They are also among the major components of sparganum excretion/secretion products (ESPs). SeANXE1 was identified in the parasite's tegument, testis, genital pore, ovary, and eggs, while SeANXB8 was found in the parasite's tegument and eggs. Plasminogen (PLG)-binding assays revealed that the two annexins could bind to human PLG in a concentration-dependent manner, which was blocked by the corresponding antibodies. These findings suggest that SeANXB8 and SeANXE1 may be involved in host-parasite interaction and may influence the host's immune response during sparganosis. They could be potential diagnosis and vaccination targets for sparganosis.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"398"},"PeriodicalIF":1.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142731807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yellow grub diseases on two seasonal killifish (Cyprinodontiformes, Rivulidae): a histopathological study. 两种季节性鳉鱼（鲤形目，鳉科）的黄蛴螬病：组织病理学研究。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-26 DOI: 10.1007/s00436-024-08420-w

L Di Cesare, M M Montes, M S Vargas, C G Barbeito, S E Plaul

{"title":"Yellow grub diseases on two seasonal killifish (Cyprinodontiformes, Rivulidae): a histopathological study.","authors":"L Di Cesare, M M Montes, M S Vargas, C G Barbeito, S E Plaul","doi":"10.1007/s00436-024-08420-w","DOIUrl":"https://doi.org/10.1007/s00436-024-08420-w","url":null,"abstract":"Yellow grub disease is a parasite infection, caused by Clinostomum spp. Metacercariae and affecting axial muscles and internal organs of freshwater fish. Killifishes live in seasonal ponds and are short-lived; in the Neotropical region they are among the most vulnerable vertebrates. The current study analyzes the possible injury and inflammatory reaction caused by parasitic infection with Clinostomum spp. metacercariae in testes, ovaries, liver, intestine and mesentery in the seasonal killifishes Trigonectes aplocheiloides and Titanolebias monstrosus. Apparently, this parasite could migrate through the circulatory system to different organs, since a cercaria was found in the lumen of a hepatic sinusoid. In all organs the inflammatory response showed dilated, congested vascular areas and infiltration of numerous inflammatory cells, mainly composed of lymphocytes, eosinophilic granular cells and macrophages. However, the most affected organs were the pancreas, ovaries and testes. In these organs, severe pancreatitis with degranulation of the pancreatic acini, scarce ovarian follicles and spermatocysts dilated with few or no seminiferous cells were observed. In seasonal killifish, yellow grub disease could cause low fertility rates or even sterility by reducing or impairing their reproductive capacity. Therefore, this type of parasitism has a considerable impact on the perpetuation of this species due to the short periods they have to breed before their habitats dry out.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"395"},"PeriodicalIF":1.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasite traits, host traits, and environment as determinants of dark diversity affinity in flea and gamasid mite assemblages from the Palearctic. 寄生虫性状、宿主性状和环境是古北区跳蚤和伽马螨群暗色多样性亲缘关系的决定因素。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-26 DOI: 10.1007/s00436-024-08408-6

Boris R Krasnov, Maxim V Vinarski, Natalia P Korallo-Vinarskaya, Irina S Khokhlova, Vasily I Grabovsky

{"title":"Parasite traits, host traits, and environment as determinants of dark diversity affinity in flea and gamasid mite assemblages from the Palearctic.","authors":"Boris R Krasnov, Maxim V Vinarski, Natalia P Korallo-Vinarskaya, Irina S Khokhlova, Vasily I Grabovsky","doi":"10.1007/s00436-024-08408-6","DOIUrl":"10.1007/s00436-024-08408-6","url":null,"abstract":"A species set in a site comprises species that are present (realized diversity) and species that could inhabit this site but are absent (dark diversity; DD). DD can be both species-driven (a species' traits preclude its presence, independently of site features) and site-driven (site features preclude the species' presence, independently of its traits). DD affinity (DDA) is a measure of species' tendencies to be absent from sites that they could inhabit or of sites' tendencies to lack species that could be present. Decomposition of DDA into DDA for species (ddasp) and for sites (ddasite) allows (a) disentangling these two mechanisms and (b) detecting species traits and site features contributing to their DDA. The species-site unified model is a Bayesian statistical model aimed at simultaneously estimating ddasp and ddasite. We applied it to flea and mite assemblages (a) within a host species across regions (component metacommunities; CtM; ddasite = ddaregion) and (b) within a region across host species (compound metacommunities; CdM, ddasite = ddahost). In CtMs, ddasp and ddaregion equally contributed to DD, whereas the relative contributions of ddasp and ddahost to DD in CdMs varied from the former being higher than the latter and vice versa. In CtM and CdM, ddasp increased in low-abundance ectoparasites exploiting a restricted number of hosts. In CtMs, ddaregion was associated with the regional environment, but we failed to find host traits affecting ddahost in CdMs. We conclude that ectoparasite species and either regions in CtMs or host species in CdMs independently contribute to DD.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"396"},"PeriodicalIF":1.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life cycle of Amblyomma calcaratum (Ixodida: Ixodidae) under laboratory conditions. Amblyomma calcaratum（Ixodida：Ixodidae）在实验室条件下的生命周期。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-25 DOI: 10.1007/s00436-024-08402-y

Hermes R Luz, Ruth M Moraes E Silva, Diego G Ramirez, Rodrigo H F Teixeira, Amália R M Barbieri, Herbet S Soares, João F Soares, Dauana M Sousa, João Luiz H Faccini, Marcelo B Labruna, Thiago F Martins

引用次数: 0

Changes in equine strongylid communities after two decades of annual anthelmintic treatments at the farm level. 马场每年使用抗蠕虫药二十年后马强直虫群落的变化。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-25 DOI: 10.1007/s00436-024-08417-5

Tetiana A Kuzmina, Alžbeta Königová, Anatoliy Antipov, Yuriy Kuzmin, Vitaliy Kharchenko, Yaroslav Syrota

{"title":"Changes in equine strongylid communities after two decades of annual anthelmintic treatments at the farm level.","authors":"Tetiana A Kuzmina, Alžbeta Königová, Anatoliy Antipov, Yuriy Kuzmin, Vitaliy Kharchenko, Yaroslav Syrota","doi":"10.1007/s00436-024-08417-5","DOIUrl":"10.1007/s00436-024-08417-5","url":null,"abstract":"The aim of this study was to assess the changes in strongylid communities of domestic horses after two decades of regular anthelmintic treatments; the changes in prevalence and relative abundance of individual strongylid species and their contribution to the observed alterations were estimated. The study was conducted in 2023; data collected in 2004 were used for comparison; 39 horses from two farms in Ukraine (22 horses in 2004 and 17 in 2023) were examined. In total, 18,999 strongylid specimens were collected by in vivo diagnostic deworming method before (in 2004, 9119 specimens) and after (in 2023, 9880 specimens) frequent application of anthelmintics. Strongylids were identified morphologically. Thirteen strongylid species were found in horses in 2023; only small strongylids (Cyathostominae) were recorded. In 2004, 21 species were found: 6 species of large strongylids (Strongylinae) and 15 of cyathostomins. Species richness (Margalef's index) and species diversity (Shannon's and Simpson's indexes) decreased over two decades on both farms; the Berger-Parker dominance index for C. nassatus dramatically increased up to 74.4. The dissimilarity of strongylid communities of 2023 and 2004 was primarily connected with the disappearance of large strongylids and rare cyathostomin species after two decades of regular anthelmintic treatments. SIMPER analysis revealed that C. nassatus and C. catinatum mostly contributed to this dissimilarity. A trend of gradual transformation of the strongylid community structure from multimodal (in 2004) to bimodal (in 2023) was observed on both farms.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"394"},"PeriodicalIF":1.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11588933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zoonotic Lagochilascaris minor and nine other parasites in a cat. 一只猫体内的人畜共患小鲎虫和其他九种寄生虫。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-22 DOI: 10.1007/s00436-024-08409-5

Renata Fagundes-Moreira, Claiton Ismael Schwartz, Felipe Auatt Batista de Sousa, Laura Berger, Fernanda Meneses Lopes, Vanessa R Barrs, Jairo A Mendoza-Roldan, Domenico Otranto, Saulo Petinatti Pavarini, Welden Panziera, João Fabio Soares

{"title":"Zoonotic Lagochilascaris minor and nine other parasites in a cat.","authors":"Renata Fagundes-Moreira, Claiton Ismael Schwartz, Felipe Auatt Batista de Sousa, Laura Berger, Fernanda Meneses Lopes, Vanessa R Barrs, Jairo A Mendoza-Roldan, Domenico Otranto, Saulo Petinatti Pavarini, Welden Panziera, João Fabio Soares","doi":"10.1007/s00436-024-08409-5","DOIUrl":"10.1007/s00436-024-08409-5","url":null,"abstract":"A case of polyparasitism in an 8-year-old female cat from Southern Brazil is reported. Among the described clinical signs, both gurltiosis and lagochilascariasis are of rare occurrence, being categorized as neglected diseases. The diagnosis of feline crural parasitic paraplegia was established through clinical signs, necropsy lesion observations, and the presence of Gurltia paralysans in histological sections of the spinal cord. Lagochilascaris minor was diagnosed with an ulcerated wound in the cervical region and further confirmed by coprology. In addition, other gastrointestinal parasites (i.e., Trichuris spp., Diphyllobothriidae, Toxocara cati, Ancylostoma spp., Cystoisospora spp., and Entamoeba spp.) were diagnosed through fecal examination and Sarcocystis felis by molecular analysis of muscle cysts. Aelurostrongylus abstrusus was detected in histological sections of lung parenchyma and confirmed by molecular analyses. The multiple parasitism detected in this case represents a unique report in terms of the number of parasites in the same individual, indicating the risk represented by stray cats as spreaders of zoonotic agents.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"392"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-33 deficiency inhibits Toxoplasma gondii infection by promoting NLRP3 inflammasome. IL-33 缺乏可通过促进 NLRP3 炎性体抑制弓形虫感染。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-21 DOI: 10.1007/s00436-024-08414-8

Yizhong Chen, Xiaoli He, Yuqin Chen, Rongzhao Zhang, Tengwen Zhang, Tao Zhang, Linqing Wu

引用次数: 0

Identification and functional characterization of multiple haemadins and an oligomeric decorsin in the Asian land leech Haemadipsa interrupta. 亚洲陆地水蛭 Haemadipsa interrupta 中多种血凝素和一种低聚脱落素的鉴定与功能特征。

IF 1.8 3区医学

Parasitology Research Pub Date : 2024-11-21 DOI: 10.1007/s00436-024-08404-w

Christian Müller, Dana Sponholz, Céline Tolksdorf, Bernhard H Rauch, Sebastian Kvist

{"title":"Identification and functional characterization of multiple haemadins and an oligomeric decorsin in the Asian land leech Haemadipsa interrupta.","authors":"Christian Müller, Dana Sponholz, Céline Tolksdorf, Bernhard H Rauch, Sebastian Kvist","doi":"10.1007/s00436-024-08404-w","DOIUrl":"10.1007/s00436-024-08404-w","url":null,"abstract":"Haematophagous leeches rely on a broad variety of bioactive compounds to secure a sufficient blood meal from their vertebrate prey. Both the primary (platelet aggregation) and secondary (blood coagulation) haemostasis are the main targets of action. The platelet aggregation inhibitor decorsin was first described in the North American leech, Macrobdella decora Say, 1824, whereas the bivalent thrombin inhibitor hirudin was originally identified in the European medicinal leech, Hirudo medicinalis Linnaeus, 1758. Hirudin blocks both the catalytic site and the fibrinogen-binding site (exosite I) of thrombin. Haemadin of the Indian land leech Haemadipsa sylvestris Blanchard, 1894, is also a highly efficient bivalent thrombin inhibitor but blocks exosite II of thrombin. So far, only the archetypal form of haemadin from H. sylvestris has been purified and functionally characterized, and two putative haemadins have been identified in the salivary transcriptome of Haemadipsa interrupta Moore, 1835, a terrestrial leech inhabiting mainly the Malayan peninsula. Here, we describe the identification of ten additional putative haemadins in the transcriptomic data set of H. interrupta, first generated by another study. Furthermore, we identified a putative oligomeric decorsin, which represents the first finding of this anticoagulant in a haemadipsid leech. Both the putative decorsin and a selection of haemadins were expressed, purified, and functionally characterized. The putative haemadins displayed a broad spectrum of thrombin-inhibitory potencies, whereas the putative oligomeric decorsin was indeed a weak inhibitor of platelet aggregation.","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"123 11","pages":"390"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11582220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0