Molecular detection of Leishmania donovani, Dirofilaria sp. "hongkongensis," and Wolbachia spp. in the dog population from tribal settlements of Western Ghats, Kerala, India.

IF 1.8 3区 医学 Q2 PARASITOLOGY
Prasanta Saini, B Sivalaxmi, H A Haritha, P A Fathima, Harish Kumar Shah, P M Ajithlal, Manju Rahi
{"title":"Molecular detection of Leishmania donovani, Dirofilaria sp. \"hongkongensis,\" and Wolbachia spp. in the dog population from tribal settlements of Western Ghats, Kerala, India.","authors":"Prasanta Saini, B Sivalaxmi, H A Haritha, P A Fathima, Harish Kumar Shah, P M Ajithlal, Manju Rahi","doi":"10.1007/s00436-025-08481-5","DOIUrl":null,"url":null,"abstract":"<p><p>Leishmaniasis and dirofilariasis are significant emerging zoonotic diseases. This study aimed to assess the prevalence of single and co-infections involving Leishmania donovani, Dirofilaria sp. \"hongkongensis\", and Wolbachia spp. among dogs from the Kani tribal settlements of Western Ghats, Kerala. In July 2023, blood samples from 75 dogs were collected and analyzed using microscopy, serology, and molecular diagnostics. Molecular analysis using kDNA-PCR and ITS1 PCR identified leishmaniasis in 39 out of 75 dog samples (52.0% [95% CI, 40.2 to 63.7], with phylogenetic analysis confirming the species as L. donovani. Additionally, 19 out of 75 dog samples (25.3% [95% CI, 16.0 to 36.7]) produced a ~ 720 bp band for nematode-specific COI amplification, which was confirmed as Dirofilaria sp. \"hongkongensis\". Among the 19 Dirofilaria-positive samples, 14 (73.7% [95% CI, 48.8 to 90.9]) tested positive for Wolbachia using the wsp primer. Among the 75 dogs screened, 12 (16% [95% CI, 08.6 to 26.3]) were co-infected with Leishmania and Dirofilaria, while 9 (12% [95% CI, 05.6 to 21.6]) were co-infected with all three parasites: L. donovani, Dirofilaria sp. \"hongkongensis\", and Wolbachia spp. The findings suggest that Kerala is gradually becoming more vulnerable to zoonoses like leishmaniasis and dirofilariasis emphasizing the need for prompt intervention and prevention measures. These results underscore the critical need for continued development of multi-pathogen detection methods, not only in regions where multiple diseases are endemic but also in areas where such diseases may emerge.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 5","pages":"50"},"PeriodicalIF":1.8000,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062044/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasitology Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00436-025-08481-5","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Leishmaniasis and dirofilariasis are significant emerging zoonotic diseases. This study aimed to assess the prevalence of single and co-infections involving Leishmania donovani, Dirofilaria sp. "hongkongensis", and Wolbachia spp. among dogs from the Kani tribal settlements of Western Ghats, Kerala. In July 2023, blood samples from 75 dogs were collected and analyzed using microscopy, serology, and molecular diagnostics. Molecular analysis using kDNA-PCR and ITS1 PCR identified leishmaniasis in 39 out of 75 dog samples (52.0% [95% CI, 40.2 to 63.7], with phylogenetic analysis confirming the species as L. donovani. Additionally, 19 out of 75 dog samples (25.3% [95% CI, 16.0 to 36.7]) produced a ~ 720 bp band for nematode-specific COI amplification, which was confirmed as Dirofilaria sp. "hongkongensis". Among the 19 Dirofilaria-positive samples, 14 (73.7% [95% CI, 48.8 to 90.9]) tested positive for Wolbachia using the wsp primer. Among the 75 dogs screened, 12 (16% [95% CI, 08.6 to 26.3]) were co-infected with Leishmania and Dirofilaria, while 9 (12% [95% CI, 05.6 to 21.6]) were co-infected with all three parasites: L. donovani, Dirofilaria sp. "hongkongensis", and Wolbachia spp. The findings suggest that Kerala is gradually becoming more vulnerable to zoonoses like leishmaniasis and dirofilariasis emphasizing the need for prompt intervention and prevention measures. These results underscore the critical need for continued development of multi-pathogen detection methods, not only in regions where multiple diseases are endemic but also in areas where such diseases may emerge.

多诺瓦利什曼、迪氏丝虫的分子检测。“香港氏体”和沃尔巴克氏体在印度喀拉拉邦西高止山脉部落定居点的狗群中。
利什曼病和双丝虫病是新出现的重要人畜共患疾病。本研究旨在评估多诺瓦利什曼原虫、Dirofilaria sp.的单一感染和合并感染的流行情况。在喀拉拉邦西高止山脉卡尼部落定居点的狗中发现了“香港氏体”和沃尔巴克氏体。2023年7月,收集了75只狗的血液样本,并使用显微镜、血清学和分子诊断进行了分析。利用kDNA-PCR和ITS1 PCR进行分子分析,在75只狗样本中鉴定出39只患有利什曼病(52.0% [95% CI, 40.2至63.7]),系统发育分析证实该物种为L. donovani。此外,75份狗样本中有19份(25.3% [95% CI, 16.0 ~ 36.7])产生了约720 bp的线虫特异性COI扩增带,确认为Dirofilaria sp.。“hongkongensis”。在19份dirofilia阳性样本中,14份(73.7% [95% CI, 48.8 ~ 90.9])使用wsp引物检测沃尔巴克氏体阳性。在筛选的75只狗中,12只(16% [95% CI, 08.6 ~ 26.3])同时感染利什曼原虫和Dirofilaria, 9只(12% [95% CI, 05.6 ~ 21.6])同时感染多诺瓦氏L.、Dirofilaria sp.。研究结果表明,喀拉拉邦正逐渐变得更容易受到利什曼病和双丝虫病等人畜共患病的影响,强调需要迅速采取干预和预防措施。这些结果强调了继续发展多种病原体检测方法的迫切需要,不仅在多种疾病流行的地区,而且在这些疾病可能出现的地区。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Parasitology Research
Parasitology Research 医学-寄生虫学
CiteScore
4.10
自引率
5.00%
发文量
346
审稿时长
6 months
期刊介绍: The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信