Identifying zoonotic risks: molecular insights into Cryptosporidium and Enterocytozoon bieneusi in pediatric cancer patients in Ahvaz, 2024.

IF 1.8 3区 医学 Q2 PARASITOLOGY
Hanieh Makipour, Ali Haghighi, Ahmad Halakou, Dian Dayer, Saeid Bitaraf, Arta Farhadi Kia, Marzieh Abbasi Nasab, Mahnaz Mori, Roya Salehi Kahyesh, Hamed Mirjalali
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Abstract

Opportunistic infections remain a major cause of morbidity and mortality among patients with immunodeficiency and malignancy. Cryptosporidium spp. and microsporidia, particularly Enterocytozoon bieneusi, are obligate intracellular parasites that can lead to severe complications and even death in immunocompromised individuals.In this cross-sectional study, 60 stool samples were collected from pediatric oncology patients aged 5-18 years who were hospitalized at Baghaei 2 Hospital in Ahvaz, southwestern Iran, in 2024. Following DNA extraction, molecular analysis was performed to detect the presence of Cryptosporidium spp. and E. bieneusi. For genotyping, amplification and sequencing of the 60-kDa glycoprotein (gp60) gene for Cryptosporidium spp. and the internal transcribed spacer (ITS) region for E. bieneusi were conducted. A total of 16 samples (26.6%) tested positive for either C. hominis or E. bieneusi. Co-infection with both pathogens was identified in two patients (3%). Statistical analysis revealed no significant association between gender and parasitic infection (P = 0.166), nor between the type of malignancy and presence of protozoa (P = 0.717). Genotyping of E. bieneusi showed the presence of BEB6, CHG3, D, and I genotypes, with BEB6 being the most prevalent. Subtyping of C. hominis revealed the presence of the IeA11G3T3 subtype. Overall, the molecular findings demonstrated a prevalence rate 26.6 % of parasitic infection among the studied population, with E. bieneusi being predominant. The detection of potentially zoonotic genotypes, particularly BEB6, underscores the likelihood of zoonotic transmission routes in these patients.

识别人畜共患风险:2024年阿瓦士儿童癌症患者隐孢子虫和比氏肠细胞虫的分子见解
机会性感染仍然是免疫缺陷和恶性肿瘤患者发病和死亡的主要原因。隐孢子虫和微孢子虫,特别是双胞虫,是专性细胞内寄生虫,可导致免疫功能低下个体的严重并发症甚至死亡。在这项横断面研究中,从2024年在伊朗西南部阿瓦兹Baghaei 2医院住院的5-18岁儿科肿瘤患者中收集了60份粪便样本。DNA提取后,进行分子分析,检测隐孢子虫和布氏伊布氏绦虫的存在。对隐孢子虫60-kDa糖蛋白(gp60)基因和布氏伊蚊内部转录间隔区(ITS)进行基因分型、扩增和测序。共有16份样本(26.6%)检测出人型伊蚊或布氏伊蚊阳性。两例患者(3%)同时感染两种病原体。性别与寄生虫感染无显著相关性(P = 0.166),恶性肿瘤类型与原虫存在无显著相关性(P = 0.717)。分型结果显示,比氏伊氏杆菌存在BEB6、CHG3、D和I基因型,其中以BEB6基因型最多。对人原疟原虫的亚型分析显示存在IeA11G3T3亚型。总体而言,分子调查结果显示,研究人群中寄生虫感染的患病率为26.6%,以布氏伊布氏绦虫为主。潜在人畜共患基因型的检测,特别是BEB6的检测,强调了这些患者中人畜共患传播途径的可能性。
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来源期刊
Parasitology Research
Parasitology Research 医学-寄生虫学
CiteScore
4.10
自引率
5.00%
发文量
346
审稿时长
6 months
期刊介绍: The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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