Parasitology Research最新文献

{"title":"G6PD deficiency and the exportome of plasmodium falciparum FCB-2: A comparative analysis utilizing one-dimensional blue-native electrophoresis and timstof mass spectrometry.","authors":"Isaac De la Rosa Cogollo, María Luisa Hernáez, Luis Felipe Clemente, Albeiro Marrugo-Padilla, Erika Rodríguez-Cavallo, Darío Méndez-Cuadro","doi":"10.1007/s00436-026-08717-y","DOIUrl":"10.1007/s00436-026-08717-y","url":null,"abstract":"<p><p>Plasmodium falciparum, the etiological agent of severe malaria in humans, remodels the surface of infected erythrocytes to evade host immune defenses and ensure its survival. During its intraerythrocytic development, P. falciparum modifies the composition of protein complexes within the host cell membrane and cytoskeleton by exporting parasitic proteins to the surface of the infected erythrocyte. However, there is limited understanding of its behavior in cells harboring protective polymorphisms against severe malaria, such as those associated with glucose-6-phosphate dehydrogenase (G6PD) deficiency, where reduced adhesion and early phagocytosis have been observed due to oxidative damage to the surface proteins of parasitized erythrocytes. Nonetheless, the impact of G6PD deficiency on the exportome proteins of P. falciparum in infected erythrocytes remains to be elucidated. In this in vitro study, we conducted a comparative analysis of the P. falciparum FCB-2 exportome in G6PD-deficient versus normal human red blood cells. Two membrane protein conglomerates were isolated at the ring, trophozoite, and schizont stages using Blue-Native electrophoresis, followed by in-gel digestion and LC-MS-tims-TOF analysis. The analysis revealed a significant decrease in the number of P. falciparum proteins identified in all asexual stages for both protein conglomerates on the surface of infected G6PD-deficient erythrocytes. A decrease in the number of effector, adhesion, trafficking, and signaling proteins that reinforce the protective mechanisms previously described was observed. These findings provide new insights into the pathways for understanding the molecular events that support G6PD's protective mechanisms against malaria.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148369492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of the parasite fauna of Serrasalmus geryi Jegú & Santos, 1988 (Characiformes: Serrasalmidae) and ecological implications of its invasion in a Neotropical floodplain.","authors":"Gabriela Michelan, Lidiany Doreto Cavalcanti, Wagner Toshio Hasuike, Atsler Luana Lehun, Gabriela Cassia Zanon Reinas, Guilherme Guerino Altoé, Rodrigo Bravin Narciso, Ricardo Massato Takemoto","doi":"10.1007/s00436-026-08716-z","DOIUrl":"10.1007/s00436-026-08716-z","url":null,"abstract":"<p><p>Biological invasions can alter ecological interactions, including parasite-host dynamics, with effects that are often gradual and difficult to predict. Serrasalmus geryi, native to the Tocantins-Araguaia basin, was recently recorded in the upper Paraná River floodplain (PIAP), Brazil. This study aimed to characterise, for the first time, the parasitic fauna of this species at an early stage of its introduction and to explore potential interactions with congeneric species. A total of 55 non-native individuals were collected between 2021 and 2024 and parasitological analyses included identification of ecto- and endoparasites. Additionally, a literature review was conducted to compare parasite assemblages with those of the native Serrasalmus maculatus and the established invasive Serrasalmus marginatus. Eleven parasite species were recorded, all reported for the first time in S. geryi, with three representing new records for the PIAP. Overall, parasite prevalence and abundance were low, and the assemblage was dominated by generalist taxa already present in the system. These findings are consistent with expectations for early invasion stages, suggesting that S. geryi is currently experiencing reduced parasitic pressure, possibly due to enemy release. The limited overlap with congeneric species indicates that parasite sharing and community restructuring are still incipient processes. This study provides a rare early-stage baseline of parasite-host interactions during a biological invasion in a natural system. The results contribute to invasion ecology by enabling future assessments of parasite acquisition, co-introduction, and transmission dynamics, and are relevant for understanding long-term ecological impacts on Neotropical floodplain ecosystems.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13442608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Toxoplasma gondii in marine fish from Tunisia: First report in the Southern Mediterranean Sea.","authors":"Ghozzi Khemissa, Ibtissem Lahmar, Anna Maria Fausta Marino, Alessandra Aparo, Rafika Challouf, Rym Ben Dhiab, Raja Chaâbane Banaoues, Najoua Haouas","doi":"10.1007/s00436-026-08714-1","DOIUrl":"10.1007/s00436-026-08714-1","url":null,"abstract":"<p><p>Toxoplasmosis, caused by Toxoplasma gondii (T. gondii), is a ubiquitous zoonotic parasitic disease increasingly reported in marine ecosystems, raising concerns about seafood contamination and potential human exposure. Consumption of raw or undercooked fish may therefore represent a potential risk to consumers. This study aimed to investigate the presence of T. gondii DNA in marine fish from the Tunisian coast and to assess their potential role as indicators of environmental contamination. A total of 559 specimens, representing 32 species, were collected and grouped into 100 pooled samples by species. Tissue samples (gills, skin/muscle, and intestine) were analyzed using real-time PCR. Overall, 16% (16/100) of pooled samples were tested positive for T. gondii, involving 13 fish species. Positive detections were observed in both wild and farmed fish, including caged Sparus aurata. Gill tissues showed the highest frequency of detection (9/16), followed by lower detection rates in skin/muscle (4/16) and intestinal (3/16) samples. Demersal species showed the highest number of positive pools, followed by pelagic and benthopelagic specimens. However, Carnivorous fish showed higher detection rates (36%) compared to omnivorous (31.6%) and herbivorous species (14.3%). This survey provides the first molecular evidence of T. gondii in marine fish from Tunisia and highlights the potential role of certain species as indicators of environmental contamination and passive carriers of this parasite within marine ecosystems.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148308953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and multi-omics analysis of extracellular vesicles from the plerocercoids of Spirometra mansoni (Cestoda: Diphyllobothriidae).","authors":"Xuan Xuan Song, Fei Gao, Gu Jin Guan, Yan Yan Zhou, Xi Zhang","doi":"10.1007/s00436-026-08710-5","DOIUrl":"10.1007/s00436-026-08710-5","url":null,"abstract":"<p><p>Parasitic extracellular vesicles (EVs) play crucial roles in the growth and development of parasites. However, we remain unaware of the EVs from the plerocercoids of Spirometra mansoni. In this study, firstly, the plerocercoids of S. mansoni were cultured in vitro to enrich EVs, which were then characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blotting. Next, the protein components and miRNAs of EVs were subsequently analysed using data-independent acquisition (DIA) and high-throughput sequencing, respectively. The results showed that EVs derived from the plerocercoids exhibit a typical double-layered membrane structure as spherical or elliptical vesicles ranging from 80 to 150 nm in diameter. The molecular markers HSP70, TSG101, HSP90, CD81, and β-Actin were significantly expressed in these EVs. Micro-proteomic analysis revealed 2,729 proteins, with functional annotation indicating that EV proteins are enriched primarily in pathways such as intracellular transport/secretion, cellular processes, and signal transduction. High-throughput miRNA sequencing revealed that the identified EVs contained 37 known miRNAs and 118 novel miRNAs. Functional enrichment analysis indicated that miRNAs primarily participate in host interactions by regulating DNA integration, apoptosis, cholesterol metabolism, and cAMP signaling pathways. Immunological analysis demonstrated that the antiserum against EVs elicited a mixed Th1/Th2 immune response, which was predominantly Th1-type. In this study, S. mansoni plerocercoid-derived EVs were successfully isolated and characterized. Systematic profiling of EV components via microproteomics and high-throughput miRNA sequencing provides a foundation for further investigations into the functional mechanisms of EVs.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13518479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and zoonotic genotyping of Enterocytozoon bieneusi in Siberian tigers (Panthera tigris altaica), Northeast China.","authors":"Hang Tian, Zhiying Cui, Zhiyou Lv, Mengqiu Liu, Yang Liu, Yilin Zhao, Weiyan Yin, Mengru Zhao, Hongyan Yu, Mengchao Zhou, Yaxian Lu, Dapeng Li, Ming Gong, Haitao Xu, Zhijun Hou","doi":"10.1007/s00436-026-08715-0","DOIUrl":"10.1007/s00436-026-08715-0","url":null,"abstract":"<p><p>Enterocytozoon bieneusi is an important zoonotic pathogen. This study examined its prevalence and genotypes in Siberian tigers in Northeast China. Fecal samples from 97 captive and 5 wild tigers were analyzed by nested PCR targeting the ITS region. Prevalence in captive tigers was 40.2% (39/97), with no significant site differences. Three genotypes were identified: D (89.7%), Peru8 (7.7%), and Type IV (2.6%), all within zoonotic Group 1. E. bieneusi was detected for the first time in a wild Siberian tiger (genotype D). The predominance of genotype D-common in poultry-suggests dietary transmission through contaminated chicken meat. These results indicate high prevalence of zoonotic genotypes in tigers, underscoring cross-species transmission risks and the need for strengthened biosecurity and surveillance.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13506582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RmTIL, a cysteine-rich protein as a putative microbiota modulator from Rhipicephalus microplus tick.","authors":"Gabriel C A Costa, Gabriel F Goulart, Fernando A A Silva, Ricardo J S Torquato, Luís F Parizi, Itabajara da Silva Vaz, Lucas Tirloni, Aparecida S Tanaka","doi":"10.1007/s00436-026-08712-3","DOIUrl":"10.1007/s00436-026-08712-3","url":null,"abstract":"<p><p>Rhipicephalus microplus, also known as the \"cattle tick\", presents worldwide distribution in tropical and subtropical regions and causes significant economic losses in livestock activity. At the present work, an immunogenic cysteine-rich protein produced in salivary glands was identified through an association of phage display and third generation sequencing (TGS) high-throughput techniques. This protein presenting a trypsin inhibitor-like (TIL) domain from R. microplus, named here as RmTIL, was expressed as a recombinant protein and submitted to a functional characterization as a serine protease inhibitor. The recombinant protein corresponding to its TIL domain portion (rRmTIL-D) inhibited human neutrophil elastase, cathepsin G, and subtilisin A, displaying inhibition constant (K_i) values of 7.0, 32.7 and 182.5 nM, respectively. RNA interference (RNAi) was employed to evaluate the effect of RmTIL-gene expression over reproductive parameters in engorged female ticks, revealing that eggs viability was significantly affected after gene silencing. Moreover, RmTIL gene silencing led to upregulation of 16S bacterial rRNA levels in ovary and eggs, while experimental infection of ticks with Gram-positive bacteria resulted in upregulated RmTIL transcripts. Following the increased bacterial burden, genes encoding proteins related to embryo development, lipid, redox, and energy metabolism pathways were modulated in eggs laid by RNAi-silenced females. Data presented here indicate that RmTIL is an immunogenic protein playing the two important roles of inhibiting host proteases and regulating tick microbiota, both crucial to the R. microplus life cycle.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13506668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic evidence of benzimidazole resistance and population structure insights in zoonotic Trichostrongylus colubriformis from Thailand.","authors":"Abigail Hui En Chan, Wallop Pakdee, Chanisara Kaenkaew, Punyanuch Kaewwongwattana, Sivapong Sungpradit, Urusa Thaenkham","doi":"10.1007/s00436-026-08713-2","DOIUrl":"10.1007/s00436-026-08713-2","url":null,"abstract":"<p><p>Trichostrongylus is a genus of zoonotic gastrointestinal nematode which is part of the strongylid group, where close contact with livestock is a risk factor for human infection. High levels of benzimidazole-resistant Trichostrongylus populations have been reported in Thailand, where the resistant allele frequency of Trichostrongylus populations were consistently higher than the resistant allele frequency of Haemonchus. Despite its zoonotic importance and the widespread use of benzimidazoles for gastrointestinal nematode infections in livestock, Trichostrongylus is often overshadowed by the more pathogenic Haemonchus. Here, we aim to assess the benzimidazole resistance status of Trichostrongylus colubriformis adults in Thailand, and investigate their genetic diversity and population structure. Using 60 T. colubriformis adults from Sukhothai and Satun, benzimidazole resistance was genotypically detected using the b-tubulin gene. Genetic diversity was investigated by amplification of the mitochondrial COI gene of representative specimens. Significant levels of benzimidazole resistance was detected, with the heterozygous (RS) genotype as the majority (81.7%). The population genetic structure revealed high genetic diversity of T. colubriformis populations and high genetic differentiation between the populations from Thailand and other countries, indicative of limited genetic exchange between populations. Despite significant levels of benzimidazole resistance in Thailand's T. colubriformis population, the population structure, where limited genetic exchange and high genetic differentiation was observed between populations, suggests that locally targeted measures could be effective to curb anthelminthic resistance spread. However, additionally surveillance of this zoonotic nematode is advocated to sustainably safeguard animal and human health.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular detection and phylogenetic characterization of Balantioides coli in lowland tapir (Tapirus terrestris).","authors":"Breno Torres da Silva, Camila Souza Carvalho Class, Rodrigo Hidalgo Friciello Teixeira, Maron Galliez, Elan Cardozo Paes de Almeida, Laís Lisboa Corrêa, Laís Verdan Dib, Maria Regina Reis Amendoeira, Alynne da Silva Barbosa","doi":"10.1007/s00436-026-08707-0","DOIUrl":"10.1007/s00436-026-08707-0","url":null,"abstract":"<p><p>As a vulnerable species to extinction, Tapirus terrestris has scarce data on gastrointestinal parasites, particularly regarding the occurrence of zoonotic ciliates such as Balantioides coli. Therefore, gastrointestinal parasites were investigated in this host using parasitological, in vitro culture, and molecular approaches. A total of 45 fecal samples were analyzed from animals under human care and from free-ranging or reintroduced individuals in southeastern Brazil. Overall parasite positivity reached 68.9%, varying among sampling sites. Protozoa from the phylum Ciliophora represented the most frequent group, occurring in 60% of samples (p ≤ 0.05). Nematode forms were identified in 31.1% of samples, predominantly as strongylid-type eggs, as well as eggs consistent with Trichuris spp. (4.4%) and members of the family Spiruridae (4.4%). Coccidian oocysts were detected in 20% of samples and were morphometrically compatible with the genus Eimeria. In vitro culture enabled the successful isolation of 20 ciliate strains, and PCR amplification targeting the ITS1-5.8SrDNA-ITS2 region confirmed B. coli in 24 samples, including isolates obtained directly from feces and in vitro cultures, corresponding to an infection rate of 53.3%. Sequence identity ranged from 98.08% to 100% compared to GenBank references. Phylogenetic analysis grouped all sequences within the B. coli clade and identified genetic variants A0 (50%), B0 (37.5%), and B1 (8.3%). Variant A0 predominated and was detected in both ex situ and in situ populations, whereas B0 and B1 showed more restricted distributions. Additionally, amoebae of the genus Entamoeba were detected in 17.8% of samples. These findings represent the first molecular confirmation of B. coli infection in T. terrestris and reinforce the epidemiological relevance of this host in the circulation of zoonotic protozoa across different ecological contexts.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148219100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence of urogenital schistosomiasis among schoolchildren in an irrigated area in Gezira State, Sudan: a brief report.","authors":"Hala Taha Elbashir, Bakri Yousf Mohamed Nour","doi":"10.1007/s00436-026-08689-z","DOIUrl":"10.1007/s00436-026-08689-z","url":null,"abstract":"<p><p>Urogenital schistosomiasis remains a major public health problem in irrigated areas of Sudan. We conducted a cross-sectional descriptive survey to determine the prevalence and intensity of Schistosoma haematobium infection and associated risk factors among basic schoolchildren in Althawra Mobi Village, Wad Madani Alkobra Locality, Gezira State. A total of 261 children aged 5-16 years were examined using urine centrifugation and microscopy. The overall prevalence was 44% (115/261), with most infections classified as light intensity (102/115). Infection was markedly higher among boys (96.5%) than among girls (3.5%). Frequent contact with irrigation canals for swimming, bathing, washing, and watering animals was significantly associated with infection, despite tap water being the main source of drinking water. These findings confirm ongoing transmission of urogenital schistosomiasis in irrigated communities of Gezira and highlight the need for sustained preventive chemotherapy, health education, and improved sanitation to reduce exposure among school-age children.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13451382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148205474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular identification and characterization of Taenia lynciscapreoli in the Eurasian lynx (Lynx lynx) and Hydatigera taeniaeformis sensu stricto in domestic cats (Felis catus) from Central Asia.","authors":"Lyudmila Lider, Rabiga Uakhit, Ainura Smagulova, Aigerim Algozhayeva, Christian Bauer, Vladimir Kiyan","doi":"10.1007/s00436-026-08709-y","DOIUrl":"10.1007/s00436-026-08709-y","url":null,"abstract":"<p><p>Investigations of taeniid fauna in felids, including the Eurasian lynx, in Central Asia have so far relied exclusively on traditional morphological methods. However, species identification based solely on morphological features can be unreliable. Therefore, the aim of the present study was to identify and characterize taeniid species from the Eurasian lynx and domestic cats in Kazakhstan using molecular techniques. Taeniid cestodes were collected from the intestines of 18 Eurasian lynx from northern and central Kazakhstan. In addition, 856 fecal samples from domestic cats in Astana, Almaty, and Kostanay were examined for cestode proglottids and eggs. Taeniid infection was detected in 7 (39%) of the lynx examined, and 5 (0.6%) of the cats were coproscopically positive for taeniid eggs or proglottids. Genomic DNA was extracted from isolated taeniid specimens obtained from each positive lynx and from taeniid-positive feline fecal samples, and mitochondrial DNA was amplified by polymerase chain reaction using species-specific primers and subsequently sequenced. Taenia lynciscapreoli was molecularly identified in the Eurasian lynx, and Hydatigera taeniaeformis sensu stricto (s.s.) in domestic cats. This study represents the first molecular identification and characterization of both taeniid species in Central Asia. Further investigations are required to identify the natural intermediate hosts of T. lynciscapreoli in Kazakhstan.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13461670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}