Pharmacy Education最新文献_第2页

Characterisation an d phytochemical screening of ethanolic extract Citrus reticulata peel and its anti-inflammatory activity using protein denaturation method 用蛋白质变性法分析柑橘果皮乙醇提取物的特征和植物化学筛选及其抗炎活性

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.16

Fitri Wulandari, Endang Sri Sunarsih, Khumairotul Musnaini

{"title":"Characterisation an d phytochemical screening of ethanolic extract Citrus reticulata peel and its anti-inflammatory activity using protein denaturation method","authors":"Fitri Wulandari, Endang Sri Sunarsih, Khumairotul Musnaini","doi":"10.46542/pe.2024.246.16","DOIUrl":"https://doi.org/10.46542/pe.2024.246.16","url":null,"abstract":"Background: Inflammation is an initial systemic response that occurs in the body and necessitates prompt management to prevent complications and death. Natural therapies, including Citrus reticulata, rich in hesperidin, a potent anti-inflammatory flavonoid, are gaining attention due to accessibility and safety.\u0000Objective: The aims of this study were to characterise Citrus reticulata peel simplicia and extract to ensure their quality, phytochemical screening and determining its anti-inflammatory activity.\u0000Method: Simplicia characterisation involves testing water content, total ash content, and acid-insoluble ash content while the extract is tested for water and total ash content. Phytochemical screening is done using the tube method, and the anti-inflammatory activity test is conducted using the protein denaturation method.\u0000Result: The extract had water content and total ash content of 14.91% and 1.89%, respectively. The ethanol extract of Citrus reticulata peel contains flavonoids, alkaloids, saponins, tannins, quinones, steroids, and terpenoids. It shows anti-inflammatory activity with an IC50 value of 132.13 µg/mL, with a difference of approximately 55% to achieve the same level of protein denaturation inhibition as positive control dexamethasone, with an IC50 value of 73.14 µg/mL.\u0000Conclusion: Citrus reticulata peel simplicia and ethanol extract met quality standards, contain flavonoids with anti-inflammatory properties, and inhibit inflammation by up to 50% at 132.13 µg/mL.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141340049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of maceration and infundation towards antioxidant capacity of leaves aqueous extracts of balik angin (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz) 浸渍和充气对巴利安金（Alphitonia incana (Roxb.) Teijsm.）

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.714

H. Ramadhan, Eka Fitri Susiani, Dyera Forestryana, Dinda Raflianti, Haura Nur Azizah, Faradilla Iedliany

{"title":"Comparison of maceration and infundation towards antioxidant capacity of leaves aqueous extracts of balik angin (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz)","authors":"H. Ramadhan, Eka Fitri Susiani, Dyera Forestryana, Dinda Raflianti, Haura Nur Azizah, Faradilla Iedliany","doi":"10.46542/pe.2024.246.714","DOIUrl":"https://doi.org/10.46542/pe.2024.246.714","url":null,"abstract":"Background: Excessive exposure to free radicals can trigger various degenerative diseases. Therefore, external antioxidants are needed from nature. One plant with the potential as a natural antioxidant is Balik Angin (Alphitonia incana (Roxb.) Teijsm. & Binn. Ex Kurz).\u0000Objective: This study aimed to determine the differences in the content of secondary metabolites and the antioxidant capacity of the aqueous extract of balik angin leaves from two different extractions.\u0000Method: The extraction methods were maceration and infundation, followed by phytochemical screening to identify secondary metabolites. The antioxidant activity assay was carried out by UV-Vis spectrophotometry by measuring the absorbance of the sample reaction with CUPRAC and FRAP reagents.\u0000Result: The phytochemical screening results of balik angin leaves infusion contained phenols, flavonoids, saponins, triterpenoids, and tannins, while the macerated extract obtained triterpenoids and tannins negatively. The quantitative assay showed the balik angin leaves infusion resulted in a powerful antioxidant category with EC50 values of 17.762 µg/mL and highly antioxidant content, which was 726 mg AAE/g extract compared to macerated aqueous extract antioxidant capacity with EC50 values of 44.719 µg/mL and 635 mg AAE/g extract antioxidant content.\u0000Conclusion: The infused aqueous extract of balik angin leaves has the potential to be a powerful antioxidant compared to the maceration product.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141339486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of genetic polymorphism of the PTP1B gene in diabetes mellitus patients taking insulin therapy in Indonesia: A narrative review 印度尼西亚接受胰岛素治疗的糖尿病患者中 PTP1B 基因多态性的分布情况：叙述性综述

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.99104

Nafrizal Fakhruzain, Syahrul Tuba

{"title":"Distribution of genetic polymorphism of the PTP1B gene in diabetes mellitus patients taking insulin therapy in Indonesia: A narrative review","authors":"Nafrizal Fakhruzain, Syahrul Tuba","doi":"10.46542/pe.2024.246.99104","DOIUrl":"https://doi.org/10.46542/pe.2024.246.99104","url":null,"abstract":"Background: Diabetes mellitus is a metabolic disease directly related to the onset of hyperglycemia, which occurs due to abnormalities in insulin secretion, insulin action, or both. Protein Tyrosine Phosphatase 1B (PTP1B) from insulin receptor signal transduction has a role in the pathogenesis of diabetes mellitus.\u0000Objective: To briefly present the distribution of genetic polymorphism of the PTP1B gene in diabetes mellitus patients taking insulin therapy.\u0000Method: A narrative review was conducted by collecting scientific journals in English from several leading platforms, such as PubMed, CrossRef, and Google Scholar, published from 2012 to 2023.\u0000Result: PTP1B can inhibit glucose transporter type 4 (GLUT4) activation in glucose uptake by cells, increasing glucose levels. The use of exogenous insulin becomes ineffective, and insulin resistance occurs. This study has limitations and needs additional prospective investigations to corroborate the findings.\u0000Conclusion: In some studies, PTP1B gene polymorphism in diabetes mellitus patients strongly correlates with insulin therapy. PTP1B gene polymorphism can cause insulin resistance because PTP1B and GLUT4 have the opposite effect.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microemulsion technology: Nanoparticle-based emulsion as a sustainable natural product formulation 微乳液技术：作为可持续天然产品配方的纳米乳液

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.124127

Mikael Ham Sembiring, Arsyadi, Taufik Riadi

{"title":"Microemulsion technology: Nanoparticle-based emulsion as a sustainable natural product formulation","authors":"Mikael Ham Sembiring, Arsyadi, Taufik Riadi","doi":"10.46542/pe.2024.246.124127","DOIUrl":"https://doi.org/10.46542/pe.2024.246.124127","url":null,"abstract":"Natural products refer to chemicals or compounds from living creatures occurring naturally in the environment. Natural commodities, such as spices, herbs, and medical remedies, are often used as additions. Natural products have many advantages, such as their environmentally sustainable nature, compatibility with the skin, affordability, and widespread availability. Nevertheless, developing natural products has several challenges, such as limited options for ingredients, functional limitations of natural substances, considerations about stability and preservation, consumer perceptions and expectations, and the variability in the composition of natural products. Microemulsions are prevalent emulsions that incorporate nanoparticles. A microemulsion is a homogeneous, transparent liquid consisting of oil, water, and a surfactant, often combined with a cosurfactant. The use of microemulsions has been extensively observed in many chemical and pharmacological applications owing to their unique attributes, such as a significant interfacial area, little interfacial tension, and the ability to solubilise and deliver hydrophobic substances. Therefore, the present review paper aims to comprehensively analyse microemulsion technology and its applications as an environmentally friendly formulation for natural products. Here, we propose strategies and research on microemulsions to overcome problems in natural product formulations.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141341829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular docking and toxicity analysis of ribosome-inactivating protein and natural chemical compounds as promising antiretroviral candidates against HIV-1 核糖体失活蛋白和天然化学化合物的分子对接和毒性分析，作为抗 HIV-1 病毒的候选药物前景广阔

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.9098

Mikael Ham Sembiring, F. J. P. Pradana, Oktavian Arya Putra, Syahrul Tuba

{"title":"Molecular docking and toxicity analysis of ribosome-inactivating protein and natural chemical compounds as promising antiretroviral candidates against HIV-1","authors":"Mikael Ham Sembiring, F. J. P. Pradana, Oktavian Arya Putra, Syahrul Tuba","doi":"10.46542/pe.2024.246.9098","DOIUrl":"https://doi.org/10.46542/pe.2024.246.9098","url":null,"abstract":"Background: HIV (human immunodeficiency virus) uses HIV-1 reverse transcriptase as the receptor to convert RNA into viral DNA. In addition, the CD4 receptor is also used by the virus to enter CD4+ T cells and subsequently replicate.\u0000Objective: To screen several proteins in Indonesian plants for the potential to bind to the CD4 receptor.\u0000Method: Molecular docking was carried out on Cluspro 2.0 specifically for protein to protein and MOE for protein to ligand.\u0000Results: The protein ligands were Cinnamomin III, Agglutinin, PAP, PAP-S, Momordin I, MAP30, Beta-luffin, Luffaculin I, Cucurmosin, DAP, Dianthin-30, Bouganin, Maize, Ricin, Abrin, Balsamin, and the bond energy values (Joules/kg.mol) were -602.8, -973.5, -511.3, -439.1, -532.2, -661.9, -487.0, -472.8, -530.9, -413.6, 444.1, -504.5, -617.2, -855.6, -883.9, -558.6, respectively. The best binding energy with CD4 was selected to identify the compound's molecule. These compounds were abrusin, abrusogenin, eicosadienoic acid, heneicosane, precatorine, and trigonelline, and the bond energy values (Joules/kg.mol) were -19.2158, -16.7057, -15.5155, -13.9632, -15.6119, and -9.2620, respectively. The toxicity test of abrusin was carried out against 18 targets, and two targets showed toxic activities.\u0000Conclusion: The content of RIP and natural chemical compounds in Abrus precatorius seeds make them the best candidate for antiretroviral therapy against HIV-1.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141338489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a dissolution test for andrographolide dispersible tablets using UV-Vis spectrophotometry 利用紫外可见分光光度法开发和验证穿心莲内酯分散片的溶出试验

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.2834

Evieta Rohana, Nuraini Ekawati, Insilia Ivanna Hapsari

{"title":"Development and validation of a dissolution test for andrographolide dispersible tablets using UV-Vis spectrophotometry","authors":"Evieta Rohana, Nuraini Ekawati, Insilia Ivanna Hapsari","doi":"10.46542/pe.2024.246.2834","DOIUrl":"https://doi.org/10.46542/pe.2024.246.2834","url":null,"abstract":"Background: Andrographolide, a terpenoid in an herbal plant, potentially has antidiabetic properties. Dispersible tablets are one of the pharmaceutical dosage forms used to improve acceptability. It is necessary to evaluate the dissolution profile to ensure the drug’s efficacy.\u0000Objective: This study aimed to determine the optimum condition of a dissolution test and its analysis for andrographolide dispersible tablets.\u0000Method: The optimisation process involves varying solvents, pH, and mole ratio of ARS-Cu(II)-andrographolide to determine the best conditions for the dissolution test of andrographolide dispersible tablets. Validation and analysis are conducted using spectrophotometry UV-Vis.\u0000Results: The optimal condition for the dissolution test was obtained with type II at 75 rpm, 900ml of citrate buffer medium with pH 3.1, and a temperature of 37±0.5ºC. The optimal analysis condition for the aliquot was obtained with Cu(II) metal and ARS reagents at pH 7, mole ratio 6:1:1.8, and a 15-minute optimum time. The maximum wavelength was 518nm using a UV-Vis spectrophotometer. The validation method has met the requirements.\u0000Conclusion: A dissolution test and its analytical method of andrographolide dispersible tablet have been validated.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141344330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotoxin-induced animal model of multiple sclerosis: Molecular mechanism focus 神经毒素诱导的多发性硬化症动物模型：聚焦分子机制

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.7389

Yandi Permana, Soni Siswanto, Nur Hidayah Kaz Abdul Aziz, B. W. Wardhani

{"title":"Neurotoxin-induced animal model of multiple sclerosis: Molecular mechanism focus","authors":"Yandi Permana, Soni Siswanto, Nur Hidayah Kaz Abdul Aziz, B. W. Wardhani","doi":"10.46542/pe.2024.246.7389","DOIUrl":"https://doi.org/10.46542/pe.2024.246.7389","url":null,"abstract":"Background: Neurotoxins can alter the central nervous system. They induce severe clinical symptoms such as encephalopathy, convulsions, muscle paralysis, and respiratory failure. Hence, the neurotoxin can mimic human central nervous system disorders such as Multiple Sclerosis (MS) to study pathophysiology and drugs of development.\u0000Objective: This mini-review compared the neurotoxins for mimicking MS in animal models.\u0000Method: This study was a narrative review using the scientific electronic databases Scopus, PubMed, and Google Scholar. All related articles by keywords animal models, multiple sclerosis, and neurotoxins were collected by YP and BW. All authors contributed to manuscript development.\u0000Result: Cuprizone, ethidium bromide, lysolecithin, Myelin Oligodendrocyte Glycoprotein (MOG), and Myelin Essential Protein (MBP) are neurotoxins for MS animal models. Cuprizone is particularly relevant for use in studies addressing toxic mechanisms of the demyelination process and studies of therapeutic interventions. Ethidium bromide causes spinal cord demyelination with distinctive features in the oligodendrocytes and astrocytes. However, it is a carcinogen agent. Lysolecithin is suitable for old animal models because the duration for remyelination is relatively longer than others. MOG and MBP are preferable to resemble MS pathophysiology in humans.\u0000Conclusion: MOG and MBP are appropriate for generating animal multiple sclerosis models for further in vivo experiments.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetic profile and incurred sample stability of hydroxychloroquine in Volumetric Absorptive Microsampling (VAMS) using high-performance liquid chromatography-photodiode array 使用高效液相色谱-光电二极管阵列进行体积吸收微量采样 (VAMS) 中羟氯喹的药代动力学特征和产生的样品稳定性

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.116123

Gregorio Fernando Hadi, Y. Harahap, Sunarsih

{"title":"Pharmacokinetic profile and incurred sample stability of hydroxychloroquine in Volumetric Absorptive Microsampling (VAMS) using high-performance liquid chromatography-photodiode array","authors":"Gregorio Fernando Hadi, Y. Harahap, Sunarsih","doi":"10.46542/pe.2024.246.116123","DOIUrl":"https://doi.org/10.46542/pe.2024.246.116123","url":null,"abstract":"Background: Hydroxychloroquine (HCQ) is a quinoline compound derived from chloroquine used to treat SLE. An in vitro stability evaluation was conducted to develop and validate the hydroxychloroquine analysis method. However, an assessment of the incurred sample stability is needed to ensure drug effectiveness, as in vitro evaluation doesn't reflect drug metabolism processes in the body.\u0000Objective: This research aims to obtain a pharmacokinetic profile of hydroxychloroquine using Volumetric Absorptive Microsampling (VAMS) as a safe sampling technique to use during the COVID-19 pandemic and evaluate the incurred sample stability of hydroxychloroquine samples.\u0000Methods: The chromatographic conditions used were column C18 (Waters, XBridge; 250 × 4.6 mm; 5μm); mobile phase acetonitrile-1% diethylamine (65:35); flow rate of 0.8 mL/min; column temperature 45°C; PDA detector analysis wavelength of 332 nm; and chloroquine as internal standard.\u0000Results: The pharmacokinetic profile of hydroxychloroquine in VAMS samples gave results that the Cmax ranged from 322.61-505.32 ng/mL with an average of 425.33 ± 65.90 ng/mL; tmax was 4 hours; mean t1/2 was 23.32 ± 9.65 hours; mean AUC0-72 was 5103.63 ± 1419.66 ng.h/mL; mean AUC0-∞ was 5763.97 ± 2155.26 ng.h/mL, and the AUC ratio was above 80%.\u0000Conclusion: The incurred sample stability of hydroxychloroquine in VAMS met the 2011 EMEA Bioanalytical Guideline requirements up to day 30.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141340670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of saluang belum (Luvunga sarmentosa) root infusion on sperm motility, viability, and testicle histology in mice (Mus musculus) 灌注沙隆贝伦（Luvunga sarmentosa）根对小鼠精子活力、存活率和睾丸组织学的影响

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.1520

Silvani Permatasari, Sherina Natalia Christin, Hairunnida Rahmatina, Gde Hary, Eka Adnyana

{"title":"The effects of saluang belum (Luvunga sarmentosa) root infusion on sperm motility, viability, and testicle histology in mice (Mus musculus)","authors":"Silvani Permatasari, Sherina Natalia Christin, Hairunnida Rahmatina, Gde Hary, Eka Adnyana","doi":"10.46542/pe.2024.246.1520","DOIUrl":"https://doi.org/10.46542/pe.2024.246.1520","url":null,"abstract":"Background: Men's infertility is frequently caused by a decrease in sperm quality. Herbal medicine, which is relatively cheap and easy to obtain, can be used to treat this condition. People in Central Borneo frequently consume boiled Saluang Belum root (Luvunga sarmentosa). L. sarmentosa root has the potential to improve sperm quality. \u0000Objective: To investigate the effects of Saluang Belum root infusion on sperm cell survival and testicle histology in mice (Mus musculus).\u0000Method: L. sarmentosa root was extracted using an infusion method, analysed and administered to mice at doses of 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW. Aquadest was used as a negative control for 15 days. On the 16th day, sperm motility and viability were assessed. The right testicles were removed and examined under a microscope at 100x and 400x magnification for five fields of view to examine mice testicle histology. \u0000Result: Infusion of L. sarmentosa contained triterpenoids, flavonoids, steroids, alkaloids, saponins, and phenols. Statistical tests of infused L. sarmentosa root on the motility, viability sperm and spermatogenic potentials of mice indicated that the doses of 200mg/kgBW, 400mg/kgBW, and 600mg/kgBW are significantly increased than control. \u0000Conclusion: L. sarmentosa root infusion increased sperm motility and viability, and the spermatogenic cells, as well as Leydig cells in mice.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141340017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A network pharmacology-based approach to explore the potentials of Saluang belum (Luvunga sarmentosa) in the male human reproductive system 以网络药理学为基础的方法探索Saluang belum（Luvunga sarmentosa）在男性生殖系统中的潜力

IF 0.5

Pharmacy Education Pub Date : 2024-06-14 DOI: 10.46542/pe.2024.246.5058

Silvani Permatasari, Erwin Prasetya Toepak, Muhammad Irmawan

{"title":"A network pharmacology-based approach to explore the potentials of Saluang belum (Luvunga sarmentosa) in the male human reproductive system","authors":"Silvani Permatasari, Erwin Prasetya Toepak, Muhammad Irmawan","doi":"10.46542/pe.2024.246.5058","DOIUrl":"https://doi.org/10.46542/pe.2024.246.5058","url":null,"abstract":"Background: Saluang belum's root (Luvunga sarmentosa) is one of the local plants in Central Kalimantan and is used by the Dayaknese to increase sexual activity. In previous research, L. sarmentosa root extract compounds have been characterised, but the bioactivity of each on male fertility has not been studied.\u0000Objective: To use bioinformatics to study the effects and mechanisms of molecular compounds of L. sarmentosa extract on the biological system of the male fertility complex.\u0000Methods: Network pharmacology methods were used to determine target essentially functional proteins in biological system networks. The molecular mechanism of L. sarmentosa compound activity was analysed using molecular docking and dynamic simulation software such as Autodock 4 and MOE.\u0000Result: The network pharmacology analysis showed that the Pregnane X receptor (PXR) protein contributed essentially to male reproductive biological functions. Based on the molecular docking simulations of PXR, 6-phenyl-3,4-dihydro-1H-1,4,5-benzotriazocin-2-one was the best. Pharmacological and toxicity parameters also showed that this compound had good characteristics. In addition, the molecular dynamics simulations showed that the best compounds could maintain complex interactions with PXR based on the resulting RMSD values.\u0000Conclusion: The compound 6-phenyl-3,4-dihydro-1H-1,4,5-benzotriazocin-2-one had the most potential as a PXR inductor. This compound also had a better activity than the standard inductor.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141342082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0