PeerJ最新文献

{"title":"Population structure and selective signature of Kirghiz sheep by Illumina Ovine SNP50 BeadChip","authors":"Ruizhi Yang, Zhipeng Han, Wen Zhou, Xuejiao Li, Xuechen Zhang, Lijun Zhu, Jieru Wang, Xiaopeng Li, Cheng-long Zhang, Yahui Han, Lianrui Li, Shudong Liu","doi":"10.7717/peerj.17980","DOIUrl":"https://doi.org/10.7717/peerj.17980","url":null,"abstract":"Objective By assessing the genetic diversity and associated selective traits of Kirghiz sheep (KIR), we aim to uncover the mechanisms that contribute to sheep’s adaptability to the Pamir Plateau environment. Methods This study utilized Illumina Ovine SNP50 BeadChip data from KIR residing in the Pamir Plateau, Qira Black sheep (QBS) inhabiting the Taklamakan Desert, and commonly introduced breeds including Dorper sheep (DOR), Suffolk sheep (SUF), and Hu sheep (HU). The data was analyzed using principal component analysis, phylogenetic analysis, population admixture analysis, kinship matrix analysis, linkage disequilibrium analysis, and selective signature analysis. We employed four methods for selective signature analysis: fixation index (Fst), cross-population extended homozygosity (XP-EHH), integrated haplotype score (iHS), and nucleotide diversity (Pi). These methods aim to uncover the genetic mechanisms underlying the germplasm resources of Kirghiz sheep, enhance their production traits, and explore their adaptation to challenging environmental conditions. Results The test results unveiled potential selective signals associated with adaptive traits and growth characteristics in sheep under harsh environmental conditions, and annotated the corresponding genes accordingly. These genes encompass various functionalities such as adaptations associated with plateau, cold, and arid environment (ETAA1, UBE3D, TLE4, NXPH1, MAT2B, PPARGC1A, VEGFA, TBX15 and PLXNA4), wool traits (LMO3, TRPS1, EPHA5), body size traits (PLXNA2, EFNA5), reproductive traits (PPP3CA, PDHA2, NTRK2), and immunity (GATA3). Conclusion Our study identified candidate genes associated with the production traits and adaptation to the harsh environment of the Pamir Plateau in Kirghiz sheep. These findings provide valuable resources for local sheep breeding programs. The objective of this study is to offer valuable insights for the sustainable development of the Kirghiz sheep industry.","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of electrical muscle stimulation on intentional binding and explicit sense of agency","authors":"Miwa Nagai, Kazuhiro Matsui, Keita Atsuumi, Kazuhiro Taniguchi, Hiroaki Hirai, Atsushi Nishikawa","doi":"10.7717/peerj.17977","DOIUrl":"https://doi.org/10.7717/peerj.17977","url":null,"abstract":"The motivating question for this study is determining whether electrical muscle stimulation (EMS)-induced movements can extend the user’s ability without reducing the sense of agency. Moreover, it is crucial to find the timing of the EMS application that is robust against individual differences and environmental changes. Previous studies have reported that the user-specific EMS-application timings, determined through explicit measures of sense of agency, would effectively shorten their reaction time in a push task while maintaining their sense of agency. However, no study has investigated EMS-application timings in relation to implicit measures of sense of agency. Intentional binding, an example of an implicit measure, refers to the phenomenon whereby the interval between an intentional action and the subsequent perceptual outcome is typically perceived to be shorter than the actual interval. By measuring this perceptual shift using a Libet clock, we have identified an EMS-application timing that accelerates the users’ push action while maintaining their sense of agency. First, to conduct the EMS-application experiment while appropriately maintaining the intentional binding effect, we designed a new push task such that a pre-action, as the base timing of the EMS-application trigger, always occurs just before the push movement. (1) We showed the difference between the action-binding effect of EMS-induced involuntary movements and voluntary push movements. Subsequently, (2) we identified the EMS application timing that significantly shifted judgments of action tasks while accelerating voluntary movements. Additionally, (3) we demonstrated that the EMS application could accelerate user pushing movement while maintaining the sense of agency at this specific application time. The proposed EMS in the novel pushing setup was found to be robustly effective against individual and environmental changes.","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of heat shock proteins in HIV-1 pathogenesis: a systematic review investigating HSPs-HIV-1 correlations and interactions","authors":"Chyntia Tresna Nastiti, Noer Halimatus Syakdiyah, R.M. Firzha Hawari, Youdiil Ophinni, Ni Luh Ayu Megasari","doi":"10.7717/peerj.18002","DOIUrl":"https://doi.org/10.7717/peerj.18002","url":null,"abstract":"Background The human immunodeficiency virus (HIV) pandemic is a global health emergency. Studies suggest a connection between heat shock proteins (HSPs) and HIV-1 infection pathogenesis. This systematic review aims to summarize HSPs’ role in HIV-1 infection pathogenesis. Materials and Methods A systematic literature search was undertaken across the National Library of Medicine (MEDLINE-PubMed), Science Direct, Web of Science, Scopus, SpringerLink, Sage, ProQuest, and Google Scholar databases, using related keywords to synthesize the HSPs’ role in HIV-1 infection pathogenesis. This literature review was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, and the protocol was registered in the Open Science Framework (OSF) database under DOI 10.17605/OSF.IO/VK3DJ. Results A database search revealed 3,332 articles, with 14 in vitro studies analysing the interaction between HSPs and HIV-1 across different cell types. HSPs are involved in HIV-1 infection through direct interactions and indirect responses to cellular stress, including HSP40, HSP70, HSPBP1, and HSP90. The study explores HSP interactions at various stages of the viral life cycle, including entry, uncoating, replication, transmission, and latency reactivation. Conclusion HSPs are crucial for the HIV lifecycle and immune response, offering the potential for new therapeutic strategies. Further research is needed to understand the clinical significance and target potential.","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Buscando Luciérnagas</i>: findings on Mexican fireflies from an 8-year virtual citizen science project.","authors":"Ek Del-Val, Ana M Flores-Gutiérrez, Regina González, Adrián Calleros","doi":"10.7717/peerj.18141","DOIUrl":"10.7717/peerj.18141","url":null,"abstract":"<p><p>Fireflies are charismatic and conspicuous animals that often evoke childhood memories, which make firefly watching an emotional and even transformative experience. Citizen science projects have the potential to enhance transformative interactions with nature. Like many insects, firefly populations are declining due to land-use change, urbanization and watershed pollution, but ecological data for this group is scarce, particularly in Mexico. Virtual Citizen Science (VCS) initiatives can serve as a scientific instrument, yield reliable and relevant scientific data, and may also offer a platform to promote broader educational outcomes. We established a VCS project to document fireflies through a Facebook page named <i>Buscando Luciernagas</i> with the following hashtag in every post <i>#veobrillar</i> in 2015. After seven years we complied the gathered data and analyzed the results. We had 647 reports in total, with strong fluctuations from year to year that were correlated with the number of posts and publicity we made each year. The largest number of sightings (319) occurred in 2021, coinciding with a change in our reporting format. Most of the reports came from central Mexico (91.5%), but we had reports from eight states and also received some international reports from nine different countries. Fireflies were most frequently seen in habitats characterized as grasslands (35%) or forests (27%), followed by gardens (17%), vacant lots (9%) and parks (5%) but also paved areas and agricultural lands were reported (3% each). Most citizen scientists reported few fireflies, 1-5 individuals (31%) while only 11% reported more than 50 fireflies per sighting. Our study can serve as a preliminary approach to explore more focused research areas in the future. For example, in areas with no sightings, we could reach out to specific local people to corroborate that there are no fireflies in the region, or in areas with high sightings we could promote conservation measures. Notably, we found it intriguing to discover numerous sightings of fireflies in urban areas, which could offer a potential avenue for further research in urban ecology.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11416087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of physical activity on anxiety levels in college students: mediating role of emotion regulation","authors":"Xinxin Sheng, Xili Wen, Jiangshan Liu, Xiuxiu Zhou, Kai Li","doi":"10.7717/peerj.17961","DOIUrl":"https://doi.org/10.7717/peerj.17961","url":null,"abstract":"Objectives To explore the effects of physical activity on anxiety levels in college students, as well as to examine the mediating role of emotion regulation. Methods A convenience sample of 1,721 college students from Shanghai, Jiangsu, Shandong, Guangxi, and Hunan was used to conduct an evaluation and a survey through the Physical Activity Rating Scale (PARS-3), the Anxiety Self-Rating Scale (SAS), and the Emotion Regulation Scale (ERQ). Results College students’ anxiety level, cognitive reappraisal, and expression inhibition scores were (44.72 ± 10.37), (30.16 ± 6.51), and (16.96 ± 4.99), respectively. There were significant grade and physical activity level differences in anxiety levels and cognitive reappraisal, and significant gender and physical activity level differences in expression inhibition among college students. Process model 4 mediated effect regression analysis showed that physical activity had a significant positive effect on cognitive reappraisal (R2 = 0.14, β = 0.04, P < 0.001), and physical activity did not have a significant expression inhibition effect (R2 = 0.17, β = 0.01, P = 0.27). Physical activity (β = −0.03, P = 0.012), cognitive reappraisal (β = −0.59, P < 0.001), and expression inhibition had a significant effect on (β = 0.57, P < 0.001) anxiety levels (R2 = 0.37). In the model effect relationship, the direct and indirect effects of physical activity on anxiety levels were −0.028 and −0.019, respectively. Conclusion Physical activity has a significant negative effect on college students’ anxiety levels. Cognitive reappraisal is a mediating variable for the effect of physical activity on anxiety levels. The higher the level of physical activity and the higher the intensity of the activity, the lower the level of anxiety.","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SNARC-like effect for tempo is consistent for fast and full tempo ranges but still controversial for slow tempo range","authors":"Alberto Mariconda, Mauro Murgia, Matteo De Tommaso, Tiziano Agostini, Valter Prpic","doi":"10.7717/peerj.18009","DOIUrl":"https://doi.org/10.7717/peerj.18009","url":null,"abstract":"Recent evidence suggested the existence of a spatial associations for music tempo with faster left-hand responses to relatively slow tempos and faster right-hand responses to relatively fast tempos. We refer to a study that systematically explored these spatial associations across different tempo ranges, revealed a clear effect only in the fast tempo range (DOI 10.3758/s13414-019-01945-8). The present study further investigated whether a spatial association exists across different tempo ranges (i.e., “full”, “slow” or “fast” tempo range). In particular, the present study was conducted aiming (1) to test the spatial associations for tempo in the full tempo range (Experiment 1) and (2) to further investigate the occurrence of this spatial associations in the slow and fast tempo ranges (Experiment 2). Experiment 1 revealed a spatial association for tempo occurs in the full tempo range (40–200 bpm). Experiment 2 confirmed this association in the fast tempo range (133–201 bpm) but showed contradictory results in the slow tempo range (40–104 bpm). This suggests that a spatial association is plausible in the slow tempo range, although further research is needed to clarify this phenomenon.","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acptp2,3 participates in the regulation of spore production, stress response, and pigments synthesis in Aspergillus cirstatus","authors":"Lei Shao, Zuoyi Liu, Yumei Tan","doi":"10.7717/peerj.17946","DOIUrl":"https://doi.org/10.7717/peerj.17946","url":null,"abstract":"Background Aspergillus cristatus was a filamentous fungus that produced sexual spores under hypotonic stress and asexual spores under hypertonic stress. It could be useful for understanding filamentous fungi’s sporulation mechanism. Previously, we conducted functional studies on Achog1, which regulated the hyperosmotic glycerol signaling (HOG) pathway and found that SI65_02513 was significantly downregulated in the transcriptomics data of ΔAchog1 knockout strain. This gene was located at multiple locations in the HOG pathway, indicating that it might play an important role in the HOG pathway of A. cristatus. Furthermore, the function of this gene had not been identified in Aspergillus fungi, necessitating further investigation. This gene’s conserved domain study revealed that it has the same protein tyrosine phosphatases (PTPs) functional domain as Saccharomyces cerevisiae, hence SI65_02513 was named Acptp2,3. Methods The function of this gene was mostly validated using gene knockout and gene complementation approaches. Knockout strains exhibited sexual and asexual development, as well as pigments synthesis. Morphological observations of the knockout strain were carried out under several stress conditions (osmotic stress, oxidative stress, Congo Red, and sodium dodecyl sulfate (SDS). Real-time fluorescence polymerase chain reaction (PCR) identified the expression of genes involved in sporulation, stress response, and pigments synthesis. Results The deletion of Acptp2,3 reduced sexual and asexual spore production by 4.4 and 4.6 times, demonstrating that Acptp2,3 positively regulated the sporulation of A. cristatus. The sensitivity tests to osmotic stress revealed that ΔAcptp2,3 strains did not respond to sorbitol-induced osmotic stress. However, ΔAcptp2.3 strains grew considerably slower than the wild type in high concentration sucrose medium. The ΔAcptp2,3 strains grew slower than the wild type on media containing hydrogen peroxide, Congo red, and SDS. These findings showed that Acptp2,3 favorably controlled osmotic stress, oxidative stress, and cell wall-damaging chemical stress in A. cristatus. Deleting Acptp2,3 resulted in a deeper colony color, demonstrating that Apctp2,3 regulated pigment synthesis in A. cistatus. The expression levels of numerous stress-and pigments-related genes matched the phenotypic data. Conclusion According to our findings, Acptp2,3 played an important role in the regulation of sporulation, stress response, and pigments synthesis in A. cristatus. This was the first study on the function of PTPs in Aspergillus fungi.","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of moderate physical activity on diabetic adhesive capsulitis: a randomized clinical trial.","authors":"Raheela Kanwal Sheikh, Amna Toseef, Aadil Omer, Anam Aftab, Muhammad Manan Haider Khan, Saeed Bin Ayaz, Omar Althomli, Aisha Razzaq, Samra Khokhar, Nazia Jabbar, Waqar Ahmed Awan","doi":"10.7717/peerj.18030","DOIUrl":"10.7717/peerj.18030","url":null,"abstract":"<p><strong>Background: </strong>Moderate physical activity (MPA) has proven advantages for glycemic control, cardiovascular health, and functional independence. However, physical activity is not part of routine conventional physical therapy (CPT) in managing diabetic adhesive capsulitis patients.</p><p><strong>Objective: </strong>To determine the effects of moderate MPA on diabetic adhesive capsulitis (AC).</p><p><strong>Methodology: </strong>A randomized control trial was conducted at the Combined Military Hospital (CMH), Muzaffarabad, Pakistan from March 2022 to October 2022. A total of <i>n</i> = 44 patients with diabetic AC, aged 40 to 65 years, HbA1c > 6.5% were enrolled. Group A received MPA and CPT, while Group B only received CPT for six weeks. The upper extremity function, pain, and range of motion were assessed at baseline, third week, and sixth week through the disability of arm, shoulder, and hand (DASH) questionnaire, numeric pain rating scale (NPRSS), and goniometer respectively.</p><p><strong>Results: </strong>The NPRS score and ROMs showed significant improvement (<i>p</i> < 0.05) in group A compared to group B with a large effect size. When comparing the mean difference of the DASH score (73 + 7.21 <i>vs</i>. 57.9 + 12.64, <i>p</i> < 0.001, Cohen's d = 1.46) was significantly improved with large effect size in group A as compared to group B.</p><p><strong>Conclusion: </strong>MPA along with CPT has positive effects on patient pain, range of motion, and disability in patients with diabetic adhesive capsulitis.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11416079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular farming expression of recombinant fusion proteins applied to skincare strategies","authors":"Guangdong Yu, Wengang Zhao, Yunpeng Wang, Nuo Xu","doi":"10.7717/peerj.17957","DOIUrl":"https://doi.org/10.7717/peerj.17957","url":null,"abstract":"This review discusses the current research progress in molecular farming technology in the field of skincare, with an emphasis on molecular farming expression strategies. The strategies of transdermal drug delivery and their advantages are also highlighted. The expression of cosmetically relevant fused proteins has become an important way to enhance the efficacy of the proteins. Therefore, we also discuss the feasibility and strategies for expressing fusion proteins in A. thaliana, specifically the fusion of Epidermal growth factor (EGF) to a cell-penetrating peptide (CPP), in which the production can be greatly enhanced via plant expression systems since these systems offer higher biosecurity, flexibility, and expansibility than prokaryotic, animal and mammalian expression systems. While the fusion of EGF to CCP can enhance its transdermal ability, the effects of the fusion protein on skin repair, melasma, whitening, and anti-aging are poorly explored. Beyond this, fusing proteins with transdermal peptides presents multiple possibilities for the development of tissue repair and regeneration therapeutics, as well as cosmetics and beauty products. As certain plant extracts are known to contain proteins beneficial for skin health, the expression of these proteins in plant systems will better maintain their integrity and biological activities, thereby facilitating the development of more effective skincare products.","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANXA2 promotes osteogenic differentiation and inhibits cellular senescence of periodontal ligament cells (PDLCs) in high glucose conditions.","authors":"Yanlin Huang, Jiaye Wang, Chunhui Jiang, Minghe Zheng, Mingfang Han, Qian Fang, Yizhao Liu, Ru Li, Liangjun Zhong, Zehui Li","doi":"10.7717/peerj.18064","DOIUrl":"10.7717/peerj.18064","url":null,"abstract":"<p><strong>Background: </strong>Periodontal ligament cells (PDLCs) are a major component of the periodontal ligament and have an important role in the regeneration of periodontal tissue and maintenance of homeostasis. High glucose can affect the activity and function of PDLCs in a variety of ways; therefore, it is particularly important to find ways to alleviate the effects of high glucose on PDLCs. Annexin A2 (ANXA2) is a calcium- and phospholipid-binding protein involved in a variety of cellular functions and processes, including cellular cytokinesis, cytophagy, migration, and proliferation.</p><p><strong>Aim: </strong>The aim of this study was to exploring whether ANXA2 attenuates the deleterious effects of high glucose on PDLCs and promotes osteogenic differentiation capacity.</p><p><strong>Methods and results: </strong>Osteogenic differentiation potential, cellular senescence, oxidative stress, and cellular autophagy were detected. Culturing PDLCs with medium containing different glucose concentrations (CTRL, 8 mM, 10 mM, 25 mM, and 40 mM) revealed that high glucose decreased the protein expression of ANXA2 (<i>p</i> < 0.0001). In addition, high glucose decreased the osteogenic differentiation potential of PDLCs as evidenced by decreased calcium deposition (<i>p</i> = 0.0003), lowered ALP activity (<i>p</i> = 0.0010), and a decline in the expression of osteogenesis-related genes (<i>p</i> = 0.0008). Moreover, β-Galactosidase staining and expression of p16, p21 and p53 genes showed that it increased cellular senescence in PDLCs (<i>p</i> < 0.0001). Meanwhile high glucose increased oxidative stress in PDLCs as shown by ROS (<i>p</i> < 0.0001). However, these damages caused by high glucose were inhibited after the addition of 1 µM recombinant ANXA2 (rANXA2), and we found that rANXA2 enhanced autophagy in PDLCs under high glucose conditions.</p><p><strong>Conclusions and discussion: </strong>Therefore, our present study demonstrates that alterations in ANXA2 under high glucose conditions may be a factor in the decreased osteogenic differentiation potential of PDLCs. Meanwhile, ANXA2 is associated with autophagy, oxidative stress, and cellular senescence under high glucose conditions.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11416082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}