Xiao Ju Wan, Meizhen Huang, Min Yu, Tao Ding, Zhihui Huang, Zhiqin Zhang, Xingwu Wu, Jun Tan
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There were no significant differences in the 2PN fertilization rate (64.98% <i>vs</i>. 67.18%, <i>p</i> = 0.362), D3 high-quality embryo rate (28.34% <i>vs</i>. 23.91%, <i>p</i> = 0.107), biochemical pregnancy rate (71.60% <i>vs</i>. 71.60%, <i>p</i> = 1), clinical pregnancy rate (65.00% <i>vs</i>. 65.00%, <i>p</i> = 1), delivery rate (50.72% <i>vs</i>. 48.44%, <i>p</i> = 0.928), miscarriage rate (7.25% <i>vs</i>. 6.25%, <i>p</i> = 1), or singleton birth weight (3,350 g <i>vs</i>. 3,200 g, <i>p</i> = 0.599) between the normal DFI (DFI < 30%) group and the high DFI (DFI ≥ 30%) group.</p><p><strong>Conclusion: </strong>Sperm DFI is weakly associated with sperm motility, forward movement, and normal sperm morphology, and the correlations are not strong. However, there is no correlation between the sperm DFI and the clinical outcomes of fresh IVF/ICSI-ET.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19451"},"PeriodicalIF":2.3000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101436/pdf/","citationCount":"0","resultStr":"{\"title\":\"Correlation of the sperm DNA fragmentation index with semen parameters and its impact on fresh embryo transfer outcomes-a retrospective study.\",\"authors\":\"Xiao Ju Wan, Meizhen Huang, Min Yu, Tao Ding, Zhihui Huang, Zhiqin Zhang, Xingwu Wu, Jun Tan\",\"doi\":\"10.7717/peerj.19451\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To evaluate the relationships between the sperm DNA fragmentation index (DFI) and semen parameters and its application in fresh embryos <i>via in vitro</i> fertilization and transfer.</p><p><strong>Methods: </strong>A retrospective propensity score matching (PSM) study was conducted on 162 cycles of <i>in vitro</i> fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and fresh embryo transfer (fresh IVF/ICSI-ET) from 2020-2024.</p><p><strong>Results: </strong>Spearman correlation analysis revealed a negative correlation between the sperm DFI and sperm motility (r = -0.44, <i>p</i> = 3.32e-08), forward movement of sperm (r = -0.46, <i>p</i> = 3.25e-09), and normal morphology of sperm (r = -0.25, <i>p</i> = 0.000); there was no correlation between the sperm DFI and male age (r = 0.08, <i>p</i> = 0.31), semen volume (r = -0.15, <i>p</i> = 0.05), sperm concentration (r = -0.16, <i>p</i> = 0.32), or male body mass index (BMI) (r = 0.02, <i>p</i> = 0.98). There were no significant differences in the 2PN fertilization rate (64.98% <i>vs</i>. 67.18%, <i>p</i> = 0.362), D3 high-quality embryo rate (28.34% <i>vs</i>. 23.91%, <i>p</i> = 0.107), biochemical pregnancy rate (71.60% <i>vs</i>. 71.60%, <i>p</i> = 1), clinical pregnancy rate (65.00% <i>vs</i>. 65.00%, <i>p</i> = 1), delivery rate (50.72% <i>vs</i>. 48.44%, <i>p</i> = 0.928), miscarriage rate (7.25% <i>vs</i>. 6.25%, <i>p</i> = 1), or singleton birth weight (3,350 g <i>vs</i>. 3,200 g, <i>p</i> = 0.599) between the normal DFI (DFI < 30%) group and the high DFI (DFI ≥ 30%) group.</p><p><strong>Conclusion: </strong>Sperm DFI is weakly associated with sperm motility, forward movement, and normal sperm morphology, and the correlations are not strong. However, there is no correlation between the sperm DFI and the clinical outcomes of fresh IVF/ICSI-ET.</p>\",\"PeriodicalId\":19799,\"journal\":{\"name\":\"PeerJ\",\"volume\":\"13 \",\"pages\":\"e19451\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101436/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PeerJ\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.7717/peerj.19451\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PeerJ","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.7717/peerj.19451","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
目的：探讨精子DNA碎片指数（DFI）与精液参数的关系及其在体外受精和移植新鲜胚胎中的应用。方法：回顾性倾向评分匹配（PSM）研究了2020-2024年162个周期的体外受精（IVF）或胞浆内单精子注射（ICSI）和新鲜胚胎移植（fresh IVF/ICSI- et）。结果：Spearman相关分析显示，精子DFI与精子活力（r = -0.44, p = 3.32e-08）、精子向前运动（r = -0.46, p = 3.25e-09）、精子正常形态（r = -0.25, p = 0.000）呈负相关；精子DFI与男性年龄（r = 0.08, p = 0.31）、精液量（r = -0.15, p = 0.05）、精子浓度（r = -0.16, p = 0.32）、男性体重指数（BMI） （r = 0.02, p = 0.98）无相关性。没有明显差异在2 pn受精率(64.98%比67.18%,p = 0.362), D3优质胚胎率(28.34%比23.91%,p = 0.107),生化怀孕率(71.60%比71.60%,p = 1),临床怀孕率(65.00%比65.00%,p = 1),交付率(50.72%比48.44%,p = 0.928),流产率(7.25%比6.25%,p = 1),或单例出生体重(3350克和3200克,p = 0.599)之间的正常发展类金融机构(DFI < 30%)组和高发展类金融机构(DFI≥30%)。结论：精子DFI与精子活力、向前运动和正常精子形态的相关性较弱，相关性不强。然而，精子DFI与新鲜IVF/ICSI-ET的临床结果之间没有相关性。
Correlation of the sperm DNA fragmentation index with semen parameters and its impact on fresh embryo transfer outcomes-a retrospective study.
Purpose: To evaluate the relationships between the sperm DNA fragmentation index (DFI) and semen parameters and its application in fresh embryos via in vitro fertilization and transfer.
Methods: A retrospective propensity score matching (PSM) study was conducted on 162 cycles of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and fresh embryo transfer (fresh IVF/ICSI-ET) from 2020-2024.
Results: Spearman correlation analysis revealed a negative correlation between the sperm DFI and sperm motility (r = -0.44, p = 3.32e-08), forward movement of sperm (r = -0.46, p = 3.25e-09), and normal morphology of sperm (r = -0.25, p = 0.000); there was no correlation between the sperm DFI and male age (r = 0.08, p = 0.31), semen volume (r = -0.15, p = 0.05), sperm concentration (r = -0.16, p = 0.32), or male body mass index (BMI) (r = 0.02, p = 0.98). There were no significant differences in the 2PN fertilization rate (64.98% vs. 67.18%, p = 0.362), D3 high-quality embryo rate (28.34% vs. 23.91%, p = 0.107), biochemical pregnancy rate (71.60% vs. 71.60%, p = 1), clinical pregnancy rate (65.00% vs. 65.00%, p = 1), delivery rate (50.72% vs. 48.44%, p = 0.928), miscarriage rate (7.25% vs. 6.25%, p = 1), or singleton birth weight (3,350 g vs. 3,200 g, p = 0.599) between the normal DFI (DFI < 30%) group and the high DFI (DFI ≥ 30%) group.
Conclusion: Sperm DFI is weakly associated with sperm motility, forward movement, and normal sperm morphology, and the correlations are not strong. However, there is no correlation between the sperm DFI and the clinical outcomes of fresh IVF/ICSI-ET.
期刊介绍：
PeerJ is an open access peer-reviewed scientific journal covering research in the biological and medical sciences. At PeerJ, authors take out a lifetime publication plan (for as little as $99) which allows them to publish articles in the journal for free, forever. PeerJ has 5 Nobel Prize Winners on the Board; they have won several industry and media awards; and they are widely recognized as being one of the most interesting recent developments in academic publishing.
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