PeerJ最新文献

{"title":"A comparative analysis of the rhizosphere microbial communities among three species of the <i>Salix</i> genus.","authors":"Tianqing Feng, Juan Li, Xiaoning Mao, Xionglian Jin, Liang Cheng, Huichun Xie, Yonggui Ma","doi":"10.7717/peerj.19182","DOIUrl":"10.7717/peerj.19182","url":null,"abstract":"<p><p>Rhizosphere microorganisms exert a significant influence in counteracting diverse external stresses and facilitating plant nutrient uptake. While certain rhizosphere microorganisms associated with <i>Salix</i> species have been investigated, numerous rhizosphere microorganisms from various <i>Salix</i> species remain underexplored. In this study, we employed high-throughput sequencing to examine the rhizosphere bacterial and fungal communities composition and diversity of three <i>Salix</i> species: <i>Salix zangica</i> (SZ), <i>Salix myrtilllacea</i> (SM), and <i>Salix cheilophila</i> (SC). Furthermore, the BugBase and FUNGuild were utilized to predict the functional roles of bacterial and fungal microorganisms. The findings revealed notable variations in the alpha and beta diversities of bacterial and fungal communities among the three <i>Salix</i> species exhibited significant differences (<i>p</i> < 0.05). The relative abundance of <i>Flavobacterium</i> was highest in the SZ samples, while <i>Microvirga</i> exhibited significant enrichment in the SM samples. <i>Microvirga</i> and <i>Vishniacozyma</i> demonstrate the highest number of nodes within their respective bacterial and fungal community network structures. The functions of bacterial microorganisms, including Gram-positive, potentially pathogenic, Gram-negative, and stress-tolerant types, exhibited significant variation among the three <i>Salix</i> species (<i>p</i> < 0.05). Furthermore, for the function of fungal microbe, the ectomycorrhizal guild had the highest abundance of symbiotic modes. This results demonstrated the critical role of ectomycorrhizal fungi in enhancing nutrient absorption and metabolism during the growth of <i>Salix</i> plants. Additionally, this findings also suggested that <i>S. zangica</i> plant was better well-suited for cultivation in stressful environments. These findings guide future questions about plant-microbe interactions, greatly enhancing our understanding of microbial communities for the healthy development of <i>Salix</i> plants.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19182"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective analysis of anti-osteoporosis medication trends among patients under 50 years old in nine major regions of China from 2016 to 2019.","authors":"Bo Chen, Liying Chen, Zhenwei Yu, Yanting Sun, Yuzhen Wang, Chen Wang, Siqi Wang, Yan Hu, Lian-Di Kan, Liu-Cheng Li","doi":"10.7717/peerj.19187","DOIUrl":"10.7717/peerj.19187","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to assess the national trends in osteoporosis prescriptions among Chinese adult outpatients aged less than 50 years with osteoporosis, from 2016 to 2019.</p><p><strong>Patients and methods: </strong>Prescriptions for adult outpatients with osteoporosis from hospitals in nine major areas were extracted from the database of the Hospitals Prescription Analysis Cooperative Project. Trends in the annual prescriptions and expenditure of osteoporosis were analyzed.</p><p><strong>Results: </strong>The number of osteoporosis hospital visits showed an increasing trend year by year from 18,412 in 2016 to 23,447 in 2019 (<i>P</i> = 0.029), and the corresponding cost increased from 2,083,872.94 Chinese yuan (CNY) to 2,643,508.59 CNY in 2019 (<i>P</i> = 0.032). The result showed that the share of newer osteoporotic use of medicines increased continuously, accounting for 34.3% of prescriptions and 26.8% of expenditures in 2019. The study found that in osteoporosis hospital visits under 50 years of age, the use of medicine increased year by year. However, bone resorption inhibitors and bone formation promoters in this group did not change significantly, accounting for only small proportion.</p><p><strong>Conclusion: </strong>The development of osteoporosis prescription in this study reflected the current situation of research in China. Meanwhile, in this study, we also investigated the epidemiology of osteoporosis in China in patients under 50 years of age, for whom the incidence of osteoporosis showed an increasing trend, which reminded us to accelerate the prevention of osteoporosis.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19187"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there hidden genetic variability in the species of <i>Steindachneridion</i> Garavello, 2005 (Siluriformes: Pimelodidae)?","authors":"Daniele Aparecida Matoso, Hallana Cristina Menezes da Silva, Augusto Luiz Ferreira Júnior, Fábio Porto-Foresti, Ricardo Utsunomia, Fernanda Dotti do Prado, Roberto Ferreira Artoni","doi":"10.7717/peerj.18193","DOIUrl":"10.7717/peerj.18193","url":null,"abstract":"<p><strong>Background: </strong>The genus <i>Steindachneridion</i>, which includes large-sized freshwater pimelodid species, is endemic to the southeastern coastal drainages of South America, specifically the Paraná River and Uruguay River basins.</p><p><strong>Methods: </strong>In this study, genetic analyses of mitochondrial DNA (mtDNA) D-loop were conducted on four species within this genus across their respective distributions: <i>Steindachneridion scriptum</i> (from the Tibagi and Uruguay rivers), <i>S. melanodermatum</i> (from the Iguaçu River), <i>S. doceanum</i> (from the Doce River), and <i>S. parahybae</i> (from the Paraíba do Sul River). <i>Zungaro zungaro</i> and <i>Brachyplatystoma rousseauxii</i> were employed as outgroups, and the topology was inferred using Bayesian Inference (BI) and maximum likelihood (ML) phylogenetic reconstruction techniques. Additionally, the sequences were analyzed to assess genetic diversity levels.</p><p><strong>Results: </strong>In contrast to the remaining species, which exhibited distinct species-specific clades, our data suggests that <i>S. scriptum</i> formed two sister clades, potentially representing distinct operational taxonomic units. Novel haplotypes were identified for each of the four species, further supporting the conclusions derived from the phylogenetic analysis. Overall, <i>Steindachneridion</i> species displayed high haplotype diversity paired with low nucleotide diversity, indicating a demographic expansion event after reduced effective population size. Nevertheless, genetic structure indexes were notably high. These findings suggest that the genetic diversity within these species may be underestimated, which has implications for both taxonomic classification and biological conservation strategies.</p><p><strong>Conclusion: </strong>In conclusion, the study of genetic diversity in four <i>Steindachneridion</i> species has revealed distinct molecular operational taxonomic units (MOTUs), which highlights the necessity for conservation efforts. The detection of new haplotypes and intraspecific variability emphasizes the urgency of implementing systematic conservation measures in the face of looming extinction threats.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18193"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EcoNicheS: enhancing ecological niche modeling, niche overlap and connectivity analysis using the shiny dashboard and R package.","authors":"Armando Sunny, Clere Marmolejo, Rodrigo Vidal-López, Fredy A Falconi-Briones, Ángela P Cuervo-Robayo, René Bolom-Huet","doi":"10.7717/peerj.19136","DOIUrl":"10.7717/peerj.19136","url":null,"abstract":"<p><p>EcoNicheS (https://github.com/armandosunny/EcoNicheS) is a comprehensive R package built on a Shiny dashboard that offers an intuitive and streamlined workflow for creating ecological niche models (ENMs) and landscape connectivity models. It incorporates tools for niche modeling, overlap analysis, and connectivity modeling, leveraging robust algorithms from the biomod2 suite. EcoNicheS is designed to simplify the technical complexities of ENMs, bridging the gap between advanced modeling techniques and user accessibility. The package offers an interactive interface for streamlined data input, model parameterization, and result visualization. Its comprehensive toolset includes occurrence data processing, pseudoabsence point generation, urbanization filters, and ecological connectivity modeling, distinguishing it from other platforms. EcoNicheS integrates innovative workflows with dynamic output visualizations while emphasizing reproducibility and comparability across statistical methods. Its practical applications span diverse research fields, including biogeography, epidemiology, evolutionary studies, climate change impacts, landscape connectivity, and biodiversity conservation. This versatility makes EcoNicheS a valuable resource for advancing in ecological and conservation science.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19136"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of endoplasmic reticulum stress in type 2 diabetes mellitus mechanisms and impact on islet function.","authors":"Zhaxicao He, Qian Liu, Yan Wang, Bing Zhao, Lumei Zhang, Xia Yang, Zhigang Wang","doi":"10.7717/peerj.19192","DOIUrl":"10.7717/peerj.19192","url":null,"abstract":"<p><p>Type 2 diabetes mellitus (T2DM) is a globally prevalent metabolic disorder characterized by insulin resistance and dysfunction of islet cells. Endoplasmic reticulum (ER) stress plays a crucial role in the pathogenesis and progression of T2DM, especially in the function and survival of β-cells. β-cells are particularly sensitive to ER stress because they require substantial insulin synthesis and secretion energy. In the early stages of T2DM, the increased demand for insulin exacerbates β-cell ER stress. Although the unfolded protein response (UPR) can temporarily alleviate this stress, prolonged or excessive stress leads to pancreatic cell dysfunction and apoptosis, resulting in insufficient insulin secretion. This review explores the mechanisms of ER stress in T2DM, particularly its impact on islet cells. We discuss how ER stress activates UPR signaling pathways to regulate protein folding and degradation, but when stress becomes excessive, these pathways may contribute to β-cell death. A deeper understanding of how ER stress impacts islet cells could lead to the development of novel T2DM treatment strategies aimed at improving islet function and slowing disease progression.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19192"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fisetin may protect early porcine embryos from oxidative stress by down-regulating GRP78 levels.","authors":"Xiu-Wen Yuan, Hao Guo, ChaoRui Wang, HeWei Ji, YongNan Xu, Xue Rui Yao, Lin Wang, QiLong Cao, Nam-Hyung Kim, Ying-Hua Li","doi":"10.7717/peerj.19198","DOIUrl":"10.7717/peerj.19198","url":null,"abstract":"<p><p>Fisetin is a natural flavonol with a variety of biological activities, including anti-inflammatory and antitumor activities. However, the effect of fisetin on mammalian oocyte and embryo development is unknown, so in this study, we used porcine oocytes as an experimental model, and added optimal concentrations of fisetin to the <i>in vitro</i> culture medium after parthenogenetic activated to investigate the effect of fisetin on porcine embryo development. It was found that 0.1 µM fisetin significantly increased the cleavage rate and blastocyst formation rate, and the quality of blastocysts was also improved. Staining results showed that the levels of reactive oxygen species (ROS), autophagy, endoplasmic reticulum stress and apoptosis were significantly reduced, while glutathione levels and mitochondrial function were significantly increased in the 0.1 µM fisetin-treated group of early porcine embryos compared with the control group. Meanwhile, fisetin decreased the expression level of the endoplasmic reticulum stress marker protein GRP78 (0.71 ± 0.19). In addition, fisetin decreased the expression of genes related to pro-apoptosis, autophagy and endoplasmic reticulum stress and increased the expression of genes related to antioxidant, pluripotency and mitochondrial. According to our results, fisetin promotes early embryonic development in porcine, and this effect may be realized by down-regulating the expression level of GRP78.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19198"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing drought resilience in durum wheat: effect of root architecture and genotypic performance in semi-arid rainfed regions.","authors":"Ridha Boudiar, Abdelhamid Mekhlouf, Yacine Bekkar, Meriem Yessaadi, Adel Bachir, Larbi Karkour, Ana Maria Casas, Ernesto Igartua","doi":"10.7717/peerj.19096","DOIUrl":"10.7717/peerj.19096","url":null,"abstract":"<p><strong>Background: </strong>Developing drought-adapted genotypes is a primary goal for achieving resilient agriculture in the Mediterranean region. Durum wheat, a widely grown crop in the drylands of the Mediterranean basin, would significantly benefit from increased drought resistance.</p><p><strong>Methods: </strong>We investigated a diverse set of 30 durum wheat varieties, including both local landraces and modern cultivars that have proven successful in Algeria. These varieties were evaluated in field trials over two consecutive years with contrasting rainfall patterns (one very dry, the other quite wet). Grain yield (PGY), yield components, and flag leaf characteristics such as area, canopy temperature, or rolling index were evaluated. Data from previous studies of root traits recorded on the same set of genotypes at seedling and adult growth stages were used to search for possible associations with grain yield and other agronomic traits measured in the current work.</p><p><strong>Results: </strong>Genotypic variation was found for all traits measured under both conditions. Grain yield and aerial biomass were reduced by 76% (from 5.28 to 1.97 Mg ha^-1) and 66% (from 15.94 to 3.80 Mg ha^-1), respectively in the dry year, whereas the harvest index increased by 32%. The breeding history of the germplasm (cultivar <i>vs</i>. landrace) had a significant effect on the traits studied. Landraces showed higher biomass only under drought (4.27 <i>vs</i>. 3.63 Mg ha^-1), whereas modern cultivars out-yielded landraces only under non-drought conditions (5.56 <i>vs</i>. 4.49 Mg ha^-1). Promising associations were found between root and agronomic traits, especially with grain yield, indicating that a profuse (large root length) and shallow (wide root angle) root system was related to increased yield of modern cultivars only in the dry year, without penalizing yield in the wet year.</p><p><strong>Conclusion: </strong>Breeding programs could improve grain yield under Algerian, semi-arid conditions, by making crosses between selected landraces with good growth potential under drought and modern cultivars, with high efficiency of biomass conversion into grain, and searching for lines with acceptable agronomic performance, which combine these desirable traits from landraces and modern cultivars, with the presence of shallow and profuse root systems.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19096"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of liquid media requirements for storing and evaluating respiratory cilia motility.","authors":"Richard Francis","doi":"10.7717/peerj.19191","DOIUrl":"10.7717/peerj.19191","url":null,"abstract":"<p><p>Mucociliary clearance is critical for maintaining normal lung function. Respiratory cilia which drive mucociliary clearance are commonly studied by measuring cilia beat frequency (CBF). There is currently significant variation within the literature regarding what is a normal value for CBF, this may be due in part to the large variety of liquid media used to suspend, maintain, and image ciliated cells. This study aimed to conduct a thorough examination to assess how media choice influences respiratory cilia motility. To accomplish this, Adult C57/BL6 mouse trachea samples were incubated in eight commonly used liquid media including: Saline, Dulbecco's Phosphate-Buffered Saline (DPBS), Hanks' Balanced Salt Solution (HBSS), Medium 199 (M199), Dulbecco's Modified Eagle's Medium (DMEM), Roswell Park Memorial Institute Medium (RPMI), Minimum Essential Medium (MEM), and Leibovitz's L-15 Medium (L-15); with or without 10% FBS supplementation. The effects of storage time (0-12 hours) and storage temperature (4 °C or room temperature) were also assessed. All media except saline were found to be equally effective in maintaining cilia function in airway samples that were freshly harvested and immediately imaged. Saline, however, significantly reduced the number of cells with motile cilia. A more complex pattern emerged when samples were stored before imaging. In saline, cilia function was significantly impaired after just one hour of storage. Samples stored in all other media showed strong maintenance of motile cilia function, with only minor changes. Notably, cilia function was better preserved with storage at 4 °C, while room temperature storage generally led to significant increases in CBF, especially in media containing FBS. Lastly, FBS supplementation was essential for maintaining cilia motility in L-15 media, as L-15 without FBS resulted in significant decreases in cilia motility following storage at either 4 °C or room temperature. In conclusion, saline should only be used if cilia are to be imaged immediately, as cilia stored in saline quickly lose motile function. All other commonly used media appear equally capable of maintaining motile cilia function for up to 12 hours when stored at 4 °C. Surprisingly, DPBS was just as effective as more expensive media in preserving ciliated samples. Storing ciliated tissue at room temperature generally leads to increased CBF, particularly in media containing FBS. Finally, L-15 media alone specifically requires the addition of 10% FBS to maintain cilia motility. These findings provide a valuable foundation for standardizing the handling, collection, and transport of ciliated samples for motile cilia assessment.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19191"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment tools for attention deficits in patients with stroke: a scoping review across components and recovery phases.","authors":"Katsuya Sakai, Takayuki Miyauchi, Junpei Tanabe","doi":"10.7717/peerj.19163","DOIUrl":"10.7717/peerj.19163","url":null,"abstract":"<p><strong>Background: </strong>Attention deficits are common in patients with stroke, making the assessment of attention functions crucial for improvement. A previous review reported on attention deficit assessments using specific components in patients with stroke. However, this study only included randomized controlled trials (RCTs) and did not encompass the attention assessments included in the observational study. Therefore, we reviewed and categorized the assessments used for attention deficits in patients with stroke according to specific attention components including RCTs and observational studies.</p><p><strong>Method: </strong>In this study, we adhered to the scoping review guidelines. The population, concept, and context of this study were stroke; attention deficits, RCTs, observational studies, and assessments; and components (focused, selective, sustained, spatial, divided, visual, and auditory attention) and phase (acute, subacute, and chronic), respectively. Two reviewers independently screened articles at the title, abstract, and full-text levels based on inclusion and exclusion criteria using four databases and the Rayyan software. Furthermore, we identified the study design, sample size, duration since stroke onset, and assessment tools were identified.</p><p><strong>Results: </strong>Out of 1,423 articles, we selected 35. The study designs included observational studies (80%) and RCTs (20%) and a total of 2,987 patients. The age range was 40.0 ± 7.7 to 83.6 ± 9.7 years. Twenty-four assessment tools were identified, mainly including the Trail Making Test Part A, Test of Everyday Attention, and other assessments (40%, 11.4%, and 62.8%, respectively). Regarding the five components of attention, there were 10 assessments were used each for sustained and selective attention (28.6%), and six each for alertness and divided attention (17.1%). Spatial attention was assessed using only one tool (2.9%).</p><p><strong>Conclusions: </strong>We identified various assessment tools for analyzing attention deficit in patients with stroke and mapped them by component. This scoping review would be useful for selecting assessment methods for patients with stroke with attention deficits.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19163"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial community composition is an important predictor of surface soil fertility across different land use types: a case study in the Three Gorges Reservoir area.","authors":"Lin Xu, Dandan Cheng, Liang Feng, Xuetian Lu, Sarah Ruffell, Hongmei Wang","doi":"10.7717/peerj.18959","DOIUrl":"10.7717/peerj.18959","url":null,"abstract":"<p><strong>Background: </strong>Surface soil is a vital component of terrestrial ecosystems and is of great importance for primary productivity. In Zhangjiachong, a small watershed in Zigui County, central China, human activity and erosion cause extensive surface soil degradation. It is still unclear as to what extent human activity influences soil fertility and soil microorganisms in this area.</p><p><strong>Methods: </strong>Soil samples were collected, during spring and autumn, across a series of land use types with different levels of human activity. We assessed soil fertility and microbial communities using 16S rRNA gene sequencing and Biolog ECO-plates.</p><p><strong>Results: </strong>The results showed that higher levels of human activity were associated with lower soil fertility and microbial metabolic activity, in addition to higher bacterial diversity. Moreover, human activity had negative effects on the relative abundances of Proteobacteria and Acidobacteriota, which were the key drivers of surface soil fertility. Conversely, stronger human activity was associated with lower abundance of Actinobacteriota. This study suggested that human activity had a negative influence on surface soil fertility, and bacterial community composition could be a good predictor of surface soil fertility.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18959"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}