PeerJ最新文献

{"title":"Psychological distress and problematic internet use among language teachers: a latent profile analysis.","authors":"Zizheng Shen, Honggang Liu, I-Hua Chen","doi":"10.7717/peerj.19707","DOIUrl":"10.7717/peerj.19707","url":null,"abstract":"<p><strong>Background: </strong>Given the limited attention to psychological distress (PD) and problematic internet use (PIU) among language teachers, the study tried to reveal the different PD profiles and their association with PIU.</p><p><strong>Methods: </strong>We first employed latent profile analysis (LPA) to identify the latent profiles within a cohort of language teachers in China and then utilized multivariate logistic regression analysis to explore the demographic characteristics associated with different PD profiles. After that, one-way analysis of variance (ANOVA) was conducted to examine the relationships between PD and PIU.</p><p><strong>Results: </strong>The LPA identified three distinct latent profiles of PD: the moderate-to-severe PD profile, the mild PD profile, and the mentally healthy profile. The results of multivariate logistic regression indicated that male and experienced language teachers were more likely to experience severe PD. The results of one way ANOVA suggested that language teachers experiencing PD reported notably higher levels of PIU, with those in the moderate-to-severe PD group scoring particularly high in internet gaming disorder.</p><p><strong>Discussion: </strong>Results of this study indicate the close association between PD and PIU in language teachers. These findings underscore the critical need to address PD among language teachers and emphasize the importance of education and training aimed at promoting teachers' appropriate internet use. Breaking the \"PD-PIU\" vicious cycle is essential for fostering better mental health and well-being within this professional group.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19707"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"West Nile Virus (<i>Orthoflavivirus nilense</i>) RNA concentrations in wastewater solids at five wastewater treatment plants in the United States.","authors":"Alessandro Zulli, Dorothea Duong, Bridgette Shelden, Amanda Bidwell, Marlene K Wolfe, Bradley White, Alexandria B Boehm","doi":"10.7717/peerj.19748","DOIUrl":"10.7717/peerj.19748","url":null,"abstract":"<p><strong>Background: </strong><i>Orthoflavivirus nilense</i>, formerly known as West Nile Virus (WNV), has become endemic to the United States since its introduction in 1999. Current surveillance methods rely primarily on mosquito pool testing, which is both costly and time-intensive. Wastewater-based epidemiology (WBE) has proven an effective method for the surveillance of various pathogens, including other orthoflaviviruses such as Dengue. WBE for WNV represents a potentially valuable surveillance approach that has so far been underexplored.</p><p><strong>Methods: </strong>A targeted droplet digital reverse transcription-polymerase chain reaction (RT-PCR) approach (ddRT-PCR) was used to measure WNV concentrations in wastewater retrospectively from five locations and in over 600 samples. Three of these locations were in communities with multiple confirmed WNV infections, while two were not. Samples were collected during periods corresponding to typical WNV seasonality (spring to fall). SARS-CoV-2 RNA was measured simultaneously to assess nucleic acid degradation during sample storage. Publicly available confirmed WNV case data were compiled from the California and Nebraska departments of public health and their weekly arboviral reports.</p><p><strong>Results: </strong>WNV RNA was detected in wastewater samples during periods of known viral circulation within a community. The adopted ddRT-PCR assay is highly specific and sensitive, and detections in wastewater solids correspond to the occurrence of cases in the season and location of sampling. WNV was detected in nine samples in three locations with known WNV clinical cases-wastewater positivity rates in these locations ranged from 3.3% to 13%. The results suggest that wastewater monitoring could serve as an effective complement to traditional surveillance methods, particularly for sentinel surveillance in locations which do not have extensive mosquito and clinical testing systems.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19748"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of macrophages in renal fibrosis and therapeutic prospects.","authors":"Di Niu, Jun Jie Yang, Dan Feng He","doi":"10.7717/peerj.19769","DOIUrl":"10.7717/peerj.19769","url":null,"abstract":"<p><p>Monocytes/macrophages are the key regulators of tissue repair, regeneration, and fibrosis. Monocyte-derived macrophages, which are characterized by high heterogeneity and plasticity, are recruited, activated, and polarized throughout the process of renal fibrosis in response to the local microenvironment. Increasing evidence suggests that phenotypic changes in macrophages are essential for chronic kidney disease (CKD) development and progression. Advanced bioinformatics and single-cell RNA sequencing analyses have revealed the critical mechanisms of macrophage iron homeostasis dysregulation and macrophage-to-myofibroblast transition (MMT), which may be a novel therapeutic target for renal fibrosis. In this review, we systematically examine the dynamic phenotype transitions of macrophages across distinct phases of kidney injury progression. Notably, we provide new insights into the multifaceted crosstalk between renal macrophages and neighboring parenchymal cells, including tubular epithelial cells, fibroblasts, podocytes, mesangial cells, and endothelial cells, mediated through diverse mechanisms, including soluble factors, extracellular vesicles, and direct cell-cell contact, and highlight the therapeutic potential of targeting macrophages.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19769"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolome profiling reveal the inhibitory effects of food preservatives on pathogenic fungi.","authors":"Zhenxia Shi, Ni Zhan, Ming Ma, Zhen Wang, Xunyou Yan, Rumeng Li, Xuejuan Liu","doi":"10.7717/peerj.19737","DOIUrl":"10.7717/peerj.19737","url":null,"abstract":"<p><p>Sec-butylamine, potassium sorbate, and citric acid were selected as preservatives to investigate their inhibitory effects on common plant pathogens-<i>Aspergillus flavus</i>, <i>Alternaria alternata</i>, and <i>Talaromyces funiculosus</i>-as well as the inhibitory mechanism of sec-butylamine against <i>A. flavus</i>. The results showed that all three preservatives significantly inhibited the growth of the tested fungi. Under the experimental conditions, 0.6% sec-butylamine, 1.2% citric acid, and 0.2% potassium sorbate completely inhibited the growth of <i>A. flavus</i>. Similarly, 0.5% sec-butylamine, 1.0% citric acid, and 0.6% potassium sorbate completely inhibited <i>A. alternata</i>, while 1.0% sec-butylamine, 1.2% citric acid, and 0.8% potassium sorbate completely inhibited <i>T. funiculosus</i>. All three preservatives exhibited strong inhibitory activity against mycelial growth, with inhibition increasing alongside concentration under <i>ex vivo</i> conditions. To explore the inhibitory mechanism of sec-butylamine on <i>A. flavus</i>, transcriptomic and metabolomic analyses were conducted on <i>A. flavus</i> mycelia before and after treatment. The results revealed that key genes such as AFLA_053390, AFLA_121370, AFLA_024930, and AFLA_041970 were significantly downregulated following sec-butylamine exposure. Additionally, AFLA_002830 and AFLA_030450 also showed reduced expression levels. Metabolomic analysis identified several metabolites associated with sec-butylamine treatment. Compounds such as (3R)-4,4-dimethyl-2-oxotetrahydro-3-furanyl β-D-glucopyranoside (Com_5857_neg), trehalose (Com_3182_neg), D-glucosamine 6-phosphate (Com_4401_neg), and sucrose (Com_494_neg) were elevated, while D-gluconic acid (Com_9540_neg), D-glucose 6-phosphate (Com_723_neg), verbascose (Com_11501_neg), and D-(-)-fructose (Com_285_neg) were reduced after treatment. This study provides a reference for the practical application of food preservatives and lays a foundation for further research into their antifungal mechanisms.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19737"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomics-based analysis of mobile genetic elements and antibiotic/metal resistance genes carried by treated wastewater.","authors":"Chahnez Naccache, Chourouk Ibrahim, Abdennaceur Hassen, Maha Mezghani Khemakhem","doi":"10.7717/peerj.19682","DOIUrl":"10.7717/peerj.19682","url":null,"abstract":"<p><p>Wastewater treatment plants in Tunisia are recognized as key locations for the spread of antibiotic and heavy metal resistance genes among bacteria. Despite the widespread presence of pollutants in these treatment systems, there is still a significant gap in our understanding of resistance dynamics. This study focused on analyzing the bacterial community and resistome-mobilome profiles of the Charguia wastewater treatment plant (WWTP). Using metagenomics sequencing, six samples from the influent, sludge, and effluent were thoroughly examined. Our research findings indicated the prevalence of <i>Proteobacteria</i> and high levels of <i>Bacteroidota</i>, <i>Firmicutes</i>, <i>Campylobacterota</i>, and <i>Patescibacteria</i>. After conducting a species level analysis, we identified important species such as <i>Pseudomonas psychrophila</i>, <i>Pseudomonas fragi</i>, <i>Pseudomonas lundensis</i>, <i>Acinetobacter johnsonii</i>, and <i>Thiothrix unzii</i> linked to antibiotic resistant genes (ARGs) like <i>mdtA</i> and <i>merR1</i> and heavy metal resistance genes (MRGs), including <i>czcA</i> and <i>cnrA</i>. Our study illustrated the persistence of specific species in the effluent due to the co-occurrence of ARGs/MRGs and mobile genetic elements (MGE). Notably, <i>IncQ</i> and <i>IncP</i> were found to be associated with <i>mdtA, mexR, arsR1</i>, and <i>merR</i>. The conclusions drawn from our research suggest that the WWTP has been potentially effective in reducing multidrug resistance.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19682"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of P fertilizer and arbuscular mycorrhizal fungi on forage legume growth, chlorophyll content and productivity.","authors":"Sanele Mpongwana, Alen Manyevere, Conference Thando Mpendulo, Johnfisher Mupangwa, Wandile Mashece, Mthunzi Mndela","doi":"10.7717/peerj.18955","DOIUrl":"10.7717/peerj.18955","url":null,"abstract":"<p><p>Soil phosphorous (P) is the most limiting plant nutrient globally, reducing forage plant productivity. Although inorganic P fertilizers are used, about 75-90% of P becomes unavailable for plant uptake, hence, the strategies to enhance P uptake acquisition, such as the use of arbuscular mycorrhizal fungi (AMF) inoculation, are crucial. A greenhouse pot experiment was conducted under controlled environmental conditions at the University of Fort Hare, where three legume species (<i>Vigna unguiculata</i>, <i>Lablab purpereus</i> and <i>Mucuna pruriens</i>) were grown for 90 days under five P fertilizer levels (0; 0.68; 1.36; 2.04 and 2.72 g P/pot) with or without AMF-inoculation, resulting in 30 treatment factorial arrangement, each replicated 4 times. Agronomic responses to P fertilization and AMF-inoculation were assessed. Plant height, stem diameter, chlorophyll content, and leaf and stem yield were significantly influenced (<i>p</i> < 0.001) by the interaction of phosphorus (P) fertilizer levels, arbuscular mycorrhizal fungi (AMF) inoculation, and legume species. Inoculated plants showed remarkable growth, reaching heights of 94.2 to 159.0 cm compared to 61.1 to 117.0 cm in uninoculated plants. Additionally, inoculated plants had stem diameters twice as large as those of uninoculated plants when grown with 1.36 g P/pot, outperforming other P fertilizer levels by day 90 across all legume species. Likewise, chlorophyll content of inoculated plants (78.1-90.7 soil plant analysis development (SPAD)) was significantly higher than uninoculated plants (56.9-69.1 SPAD) at 1.63 P g/pot compared to 0, 0.68, 2.04 and 2.72 g P/pot. Moreover, inoculated plants attained relatively higher leaf (123.3-144.0 g/pot) and stem yield (75.2-121.8 g/pot) than uninoculated plants at 1.36 g P/pot compared to 0, 0.68, 2.04 and 2.72 g P/pot. Overall, AMF-inoculation improved growth and productivity of forage legumes, but its effects depended on the P fertilizer level, with 1.36 g P/pot being the potential optimum fertilizer rate for soil nutrition of legume pastures.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e18955"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Andean plump toad of the genus <i>Osornophryne</i> (Anura: Bufonidae) from Cerro Candelaria, Ecuador.","authors":"Juan P Reyes-Puig, Miguel A Urgiles-Merchán, H Mauricio Ortega-Andrade, Diego F Cisneros-Heredia, Julio C Carrión-Olmedo, Mario H Yáñez-Muñoz","doi":"10.7717/peerj.19760","DOIUrl":"10.7717/peerj.19760","url":null,"abstract":"<p><p>The amphibian genus <i>Osornophryne</i> is endemic to the northern Andes of South America and has long been considered rare. Recent explorations in the humid montane forests of the upper Pastaza Valley have uncovered previously unknown species. Here, we describe a new Andean toad species from the central Ecuadorian Andes, identified through genetic analyses and distinctive morphological and cranial traits. <i>Osornophryne backshalli</i> sp. nov., from Cerro Candelaria in the upper Pastaza River basin, is closely related to <i>O. sumacoensis</i> from Sumaco Volcano. This new species is characterized by a uniquely short fifth toe relative to toes I-III, triangular papillae on the snout tip, an occipital fold, large subconical and conical warts on the body, dorsal surfaces Brownish Olive with Spectrum Yellow and Light Neutral Gray flecks, ventral surfaces Brownish Olive with Spectrum Yellow bright blotches. Our phylogenetic analyses revise the genus taxonomy by delimiting two well-supported clades: the <i>Osornophryne bufoniformis</i> species group and the <i>Osornophryne guacamayo</i> species group. Furthermore, we show that the Pastaza River does not constitute a geographical barrier for <i>Osornophryne</i> distribution. These findings emphasize the value of continued exploration to expand our understanding of this genus in the humid montane forests of the Ecuadorian Andes.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19760"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of soy isoflavones on menopausal symptoms in perimenopausal women: a systematic review and meta-analysis.","authors":"Haodi Luan, Qianqian Liu, Yahui Guo, Hua Fan, Sileng A, Jing Lin","doi":"10.7717/peerj.19715","DOIUrl":"10.7717/peerj.19715","url":null,"abstract":"<p><p>Soy isoflavones are phytoestrogens found mainly in soy and its derivatives. Given their estrogen-like and antioxidant-inhibiting inflammatory effects, they have been hypothesized to be effective in treating menopausal symptoms. We conducted a systematic review in accordance with the PRISMA guidelines. In October 2024, we screened 2,099 articles, of which 12 were eligible for meta-analysis, and found that soy isoflavones were effective for treating menopausal symptoms (seven studies, 533 participants, Hedges' g = -0.25, 95% CI [-0.42 to -0.08], <i>p</i> = 0.00). Soy isoflavones showed significant effects on headache (three studies, 340 participants, Hedges' g = -0.38, 95% CI [-0.60 to -0.17], <i>p</i> = 0.00), psychosocial symptoms (five studies, 416 participants, Hedges' g = -0.29, 95% CI [-0.48 to -0.10], <i>p</i> = 0.00), palpitation (three studies, 356 participants, Hedges' g = -0.42, 95% CI [-0.63 to -0.22], <i>p</i> = 0.00), and depression (four studies, 748 participants, Hedges' g = -0.72, 95% CI [-1.17 to -0.28], <i>p</i> = 0.00), but no significant treatment effect on paresthesia symptoms, fatigue symptoms, physical symptoms, hot flushes, excessive sweating, insomnia, and vasomotor symptoms was observed. However, our results should be interpreted with caution owing to the small sample size. More trials should be conducted in the future to validate our findings.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19715"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study of salivary cortisol and glutamate after the cold pressor task in healthy adults.","authors":"Roxaneh Zarnegar, Angeliki Vounta, Arisara Amrapala, Sara S Ghoreishizadeh","doi":"10.7717/peerj.19625","DOIUrl":"10.7717/peerj.19625","url":null,"abstract":"<p><p>Nociception related salivary biomolecules can be a useful future aid in the assessment of acute pain. We have investigated changes in the levels of two salivary biomolecules, glutamate and cortisol, following the induction of acute cold pain using the cold pressor task (CPT). Saliva samples were collected from 18 healthy volunteers before, immediately after and then, every 10 minutes for one hour after CPT. Statistical analysis of the biomolecule concentrations across all participants and time points were done. This showed significant differences between salivary cortisol concentration before (median 0.14 µg/dL, Interquartile Range (IQR) = 0.1) and 10 minutes after termination of CPT (median 0.34 µg/dL, IQR = 0.4, <i>p</i> = 0.007). Male participants exhibited a greater increase in cortisol concentration after cold pain compared to females. The timeline and pattern of the rise in salivary cortisol concentration in this study are consistent with existing literature. Salivary glutamate concentration fluctuated but none of the changes were statistically significant except at <i>t</i> =  + 50 minutes, when the concentration had dropped below baseline. The findings do not support the use of glutamate as a useful biomarker in acute pain despite evidence that plasma and salivary glutamate levels are higher in people with chronic pain conditions such as migraine and temporomandibular disorder.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19625"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining echocardiography with carotid ultrasound parameters in predicting major adverse cardiovascular events among older individuals with coronary artery disease.","authors":"Tianli Jia, Xiaoxiao Li, Qianqian Xie","doi":"10.7717/peerj.19688","DOIUrl":"10.7717/peerj.19688","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the predictive value of combining echocardiography with carotid ultrasound for major adverse cardiovascular events (MACE) among older individuals with coronary artery disease.</p><p><strong>Methods: </strong>A total of 138 elderly individuals diagnosed with coronary artery disease and admitted to our facility from June 2020 to June 2021 were included in this study. These patients were categorized into two groups: a non-MACE group consisting of 84 patients and a MACE group with 54 patients, based on whether they experienced MACE within three years following their discharge. Additionally, a validation cohort of 141 patients was assembled, which was also divided into a non-MACE group with 90 patients and a MACE group with 51 patients. Upon admission, all participants underwent both transthoracic echocardiography and carotid ultrasound assessments. We then conducted a comparative analysis of the ultrasound parameters between the two groups.</p><p><strong>Results: </strong>The MACE group had higher left ventricular end-diastolic diameter (LVEDD), carotid intima-media thickness (IMT), and Crouse score, and lower left ventricular ejection fraction (LVEF) and A-wave velocity (all <i>P</i> < 0.05). LVEDD, IMT, and Crouse score were risk factors for MACE, while LVEF and A value were protective factors against MACE. Derivation area under the curve (AUC) = 0.755; validation AUC = 0.754.</p><p><strong>Conclusion: </strong>Echocardiography and carotid ultrasound parameters can be used to predict the occurrence of MACE within 3 years in elderly patients with coronary artery disease, and combined detection can effectively improve the accuracy of prediction.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19688"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}