Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"Genome-wide identification and characterization of the carbonic anhydrase gene family reveals carbonate alkalinity adaptability in Chinese mitten crab (Eriocheir sinensis)","authors":"Yulin Bai ,&nbsp;Junlei Ma ,&nbsp;Zihan Fan ,&nbsp;Anqi Wang ,&nbsp;Dongpo Xu","doi":"10.1016/j.cbpc.2025.110255","DOIUrl":"10.1016/j.cbpc.2025.110255","url":null,"abstract":"<div><div>Carbonic anhydrase (CA) is an essential zinc metalloenzyme that plays a crucial role in the physiological process of acid-base regulation in aquatic species. The <em>CA</em> gene family has been characterized in multiple species, but a comprehensive analysis of the <em>CA</em> gene family in <em>Eriocheir sinensis</em> is yet to be conducted. In this study, we identified members of the <em>CA</em> gene family from the <em>E. sinensis</em> genome and analyzed their genomic physical distribution, structural characteristics, expression patterns, and cellular localization. The results revealed a total of 15 <em>CA</em> genes in <em>E. sinensis</em>, distributed across 12 chromosomes, with tandem duplication observed on chr43. Phylogenetic analysis indicated that the 39 <em>CA</em> genes from <em>E. sinensis</em>, <em>Procambarus clarkii</em>, and <em>Portunus trituberculatus</em> can be classified into 4 clades and 11 subclades, with events of gene duplication and loss observed. Transcriptome expression analysis suggested that the <em>EsiCA7</em> gene might play a dominant role in the acid-base regulation of <em>E. sinensis</em>. Furthermore, subcellular localization experiments for the <em>EsiCA1</em> and <em>EsiCA7</em> genes confirmed the predictions made by bioinformatics analysis. In conclusion, this study provides a theoretical basis and molecular targets for further research on the function of the <em>CA</em> gene family and the mechanism of alkalinity tolerance in <em>E. sinensis</em>.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110255"},"PeriodicalIF":3.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quinoxyfen 1995-2019 is the story's end? Evaluation of its adverse effects on head size and nervous system genes involved in synaptic maturation","authors":"Annamaria Iannetta ,&nbsp;Silvana Zugaro ,&nbsp;Giovanni Angelozzi ,&nbsp;Francesca Mazza ,&nbsp;Marco Minacori ,&nbsp;Tommaso Silvestrini ,&nbsp;Elisabetta Benedetti ,&nbsp;Monia Perugini","doi":"10.1016/j.cbpc.2025.110252","DOIUrl":"10.1016/j.cbpc.2025.110252","url":null,"abstract":"<div><div>In 2013 quinoxyfen (QXY) was included in the list of priority hazard pollutants of the European Water Framework Directive due to its toxicity to aquatic organisms. The use of products formulated containing QXY was banned from all commercials starting from 27th June of 2019. QXY is an organic pollutant with potential persistence, bioaccumulation and this must not lower attention to this pollutant. To date the effects of this compound on development are not completely clear, thus this research tries to elucidate the adverse effects of QXY on zebrafish development. The study aims to understand the toxicological effects of QXY using the zebrafish as in vivo model and performing toxicological and molecular investigations. Considering the FET test results two sublethal concentrations, 0.4 mg/L and 0.8 mg/L were chosen for subsequent analysis. The expression of the <em>gad1b, cyp19a1b, shank3a, nrxn1a</em> and <em>c-fos</em> genes, involved in the development of the nervous system and the regulation of synaptic transmission, were evaluated. To confirm the potential neurotoxic effects of the treatment on the development of the central nervous system, both a transgenic <em>Tg(neuroD:gfp ia50)</em> line was used for confocal microscopy and Orange Acridine was used on wild type larvae to assess the presence of neuronal apoptosis. The results showed sub-lethal alterations, particularly affecting craniofacial and brain development highlighting as QXY may represent a possible endocrine disruptor able to induce severe cartilage defects, small head and tremor phenotype in zebrafish larvae and a strong modulation of the selected genes.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110252"},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of Qiukui Taoren Recipe against acute renal injury by inhibiting oxidative stress, inflammation and apoptotic pathways","authors":"Jing Huang ,&nbsp;Yuxin Wang ,&nbsp;Lixin Feng ,&nbsp;Meng Jin ,&nbsp;Zhaoqing Meng ,&nbsp;Xiaodi Dong ,&nbsp;Jimiao Shan ,&nbsp;Rongchun Wang ,&nbsp;Kechun Liu","doi":"10.1016/j.cbpc.2025.110253","DOIUrl":"10.1016/j.cbpc.2025.110253","url":null,"abstract":"<div><div>Okra and peach kernel, named Qiukui and Taoren in China, respectively, which can be used for medicine and food, are widely applied for the treatment of multifaceted diseases. However, the protective effects against acute kidney injury (AKI) and the underlying mechanisms have not been fully assessed. The aim of this study was to assess the protective effect of Qiukui Taoren Recipe (QT), and the potential molecular mechanisms in AKI using network pharmacology prediction, <em>in vivo</em> and <em>in vitro</em> experiment. There were 42 active components of QT detected by HPLC-Q-TOF/MS, and network pharmacology showed that these active components mainly could act with 63 core targets such as TNF-α, Caspase-3, BCL2, STAT3, PPARγ, and regulated 110 signaling pathways for alleviating AKI. In addition, QT reduced periocular oedema, renal malformations and size reduction, partially restore renal excretory function, and reduced the accumulation of proteinuria in zebrafish AKI model induced by caristolochic acid (AA). QT could upregulate the mRNA expression of <em>Nephrin</em> and oxidative stress genes (<em>Nrf2</em>, <em>HO-1</em>, <em>PPARγ</em>), and downregulate inflammation- and apoptosis-related genes (<em>IL-1β</em>, <em>IL-6</em>, <em>TNF-α</em>, <em>NF-κB</em>, <em>Caspase-3</em>, <em>P53</em>). Furthermore, QT inhibited ROS elevation and apoptosis activated by cisplatin (CDDP) in HEK293T cells. Western blotting results showed that QT could enhance the protein expression of nephrin, and inhibited the protein expression of Kim-1, IL-18, NLRP3, STAT3, P53 and Caspase-3. Overall, these results indicated the protective effect of QT against AKI was due to the inhibition of oxidative stress, inflammation-and apoptosis-related pathways.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110253"},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of allelochemicals from three submerged plants and the inhibitory effects of organic acids on cyanobacteria (Cylindrospermopsis raciborskii)","authors":"Junkai Zhang,&nbsp;Tinghui Min,&nbsp;Jinyu Wang,&nbsp;Zhikun Li,&nbsp;Xi Zhu,&nbsp;Shu-Xuan Liang","doi":"10.1016/j.cbpc.2025.110251","DOIUrl":"10.1016/j.cbpc.2025.110251","url":null,"abstract":"<div><div>C<em>yanobacteria</em> (<em>C. raciborskii</em>)<em>,</em> as a type of harmful algal bloom cyanobacteria, has strong invasiveness and has received widespread attention in eutrophic waters<em>.</em> Using allelopathic organic acids from submerged plants offers a promising strategy for controlling its blooms. This study investigated inhibitory effects of four plant-derived organic acids, including ellagic acid, gallic acid, caffeic acid, and p-hydroxybenzoic acid, on <em>C. raciborskii</em> through growth parameters, chlorophyll-a content, photosynthetic systems, and antioxidant responses<em>.</em> The results indicated that these organic acids significantly altered the concentrations of photosynthetic pigments, alkaline phosphatase (ALP) activity, and antioxidant enzyme activities of <em>C. raciborskii</em>. Different concentrations of organic acids had a significant influence on the intracellular photosynthetic pigment levels of <em>C. raciborskii</em>. These results revealed the reduction of chlorophyll-a was dose-dependent and time-dependent under the action of ellagic acid and gallic acid. All tested acids markedly elevated the alkaline phosphatase activity (248–398 % of the control group). Organic acids also significantly affected the activities of superoxide dismutase, catalase, and glutathione peroxidase, while increasing the level of malondialdehyde, indicating enhanced cellular oxidative stress. These findings suggest that organic acids inhibit photosynthetic pigment synthesis, phosphorus uptake, and antioxidative defenses, ultimately suppressing the growth of <em>C. raciborskii</em>.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110251"},"PeriodicalIF":3.9,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental and neurobehavioral toxicity of di-(2-ethylhexyl) phthalate (DEHP) in zebrafish larvae.","authors":"Garima Jindal, Anuradha Mangla, Poonam Goswami, Mehjbeen Javed, Mohd Anas Saifi, Iqra Mazahir, Padmshree Mudgal, Basir Ahmad, Sheikh Raisuddin","doi":"10.1016/j.cbpc.2025.110254","DOIUrl":"https://doi.org/10.1016/j.cbpc.2025.110254","url":null,"abstract":"<p><p>Di-ethyl hexyl phthalate (DEHP), a plasticizer used in medical devices and cosmetics, easily leaches into the environment due to its weak chemical bonds with plastics. Humans and other organisms are exposed to DEHP through inhalation, ingestion, and dermal contact, inflicting toxic effects like neurotoxicity, hepatotoxicity, and reproductive toxicity. However, there is a lack of information on its developmental toxicity, and its role in demyelination is also not well understood. We explored the adverse effects of DEHP during the early developmental stage of zebrafish larvae, focusing on its deteriorative impact on the nervous system through demyelination at various concentrations (10, 20, 50, 100, and 200 ppm). Cuprizone was used as a positive control compound. Various markers of oxidative stress, hormonal changes, and altered expression of myelin-related protein were measured in larvae exposed to DEHP. Results showed that higher concentrations of DEHP had lethal effects on zebrafish larvae. The activity of acetylcholinesterase (AChE) and the level of reduced glutathione (GSH) were significantly inhibited (p < 0.01), while lipid peroxidation (LPO) and glutathione S-transferase (GST) activity were significantly increased (p < 0.001). Additionally, cortisol and thyroxine levels were notably decreased, together with decreased mRNA and protein expression of the myelin sheath and its cell markers such as myelin basic protein (MBP), SRY-box transcription factor (SOX10), and oligodendrocyte lineage transcription factor 2 (Olig2) in the DEHP-treated larvae. Overall, this study offers an understanding of the developmental neurotoxicity of DEHP and its behavioral and demyelinating effects in zebrafish larvae during early developmental stages.</p>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":" ","pages":"110254"},"PeriodicalIF":3.9,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roundup Transorb reduces growth and expression of somatotropic axis genes in the silverside Odontesthes argentinesis fish larvae","authors":"Maidana Idiarte ,&nbsp;Vinicius Farias Campos ,&nbsp;Amanda Weege da Silveira Martins ,&nbsp;Nivia Maria Streit ,&nbsp;Gilson de Mendonça ,&nbsp;Ricardo Berteaux Robaldo","doi":"10.1016/j.cbpc.2025.110256","DOIUrl":"10.1016/j.cbpc.2025.110256","url":null,"abstract":"<div><div>Despite the use of herbicides has led to substantial increases in agricultural production, it has also introduced a series of environmental challenges that pose threats to the survival of non-target species in both terrestrial and aquatic ecosystems. Species of the genus <em>Odontesthes</em> have significant economic importance as they are valuable resources for artisanal fishing and local populations' income. Moreover, these organisms serve as crucial biological models in ecotoxicological and genetic research. Given the ecological and economic importance of <em>Odontesthes argentinensis</em>, this research aimed to investigate how the gene expression of the somatotropic axis (<em>gh, ghr1, ghr2, igf1,</em> and <em>igf2</em>) is impacted by the herbicide Roundup Transorb (RT), validating them as reliable biomarkers of exposure. <em>O. argentinensis</em> larvae were exposed to glyphosate at concentrations of 0.36, 1.8, and 3.6 mg/L for 96 h. The results indicate that there was a reduction in the expression of all analyzed genes in animals exposed to RT, resulting in decreased body growth. The expression of genes in the somatotropic axis was thus established as an effective biomarker for assessing exposure to Roundup Transorb herbicide.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110256"},"PeriodicalIF":3.9,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of metformin against volatile organic compounds-induced developmental toxicity in zebrafish embryos","authors":"Jieru Lu ,&nbsp;Zhuoyan Li ,&nbsp;Xuehua Ruan ,&nbsp;Lihui Jin ,&nbsp;Xiaogang Yu ,&nbsp;Xin Zhou ,&nbsp;Jianyuan Zhao ,&nbsp;Yurong Wu ,&nbsp;Sun Chen ,&nbsp;Kun Sun ,&nbsp;Jing Sun","doi":"10.1016/j.cbpc.2025.110250","DOIUrl":"10.1016/j.cbpc.2025.110250","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) are widespread environmental pollutants associated with various health risks, including developmental toxicity. Our previous studies revealed a correlation between indoor VOCs exposure and an increased risk of congenital heart diseases (CHDs), which was confirmed by establishing a zebrafish exposure model. Metformin (MET), a classic hypoglycemic drug, has been identified to possess multiple properties. Based on previous research on the protective effects of metformin on air pollution, we propose a hypothesis of using metformin as protective agent against VOCs-induced developmental toxicity in zebrafish embryos and explore the underlying molecular mechanisms.</div><div>The zebrafish embryos were exposed to the VOCs mixture (128 mg/L) alone or in combination with different concentrations of MET (0.5, 1.0 and 2.0 mg/mL) for 120 h. Toxicological indicators such as survival rate, hatching rate and morphological abnormalities (spinal curvature, yolk sac edema and body length reduction) were significantly alleviated by MET, especially in concentrations of 0.5 mg/mL. MET treatment effectively mitigated both cardiovascular impairments (pericardial edema and bradycardia) and neurodevelopmental deficits (locomotor activity). Transcriptomic profiling revealed MET-mediated modulation of apoptosis-related genes and cell cycle regulators. Pathway analyses identified significant enrichment in neurodevelopmental and cardiovascular functions, corroborated by acridine orange staining showing reduced apoptosis.</div><div>Our findings demonstrate MET's anti-apoptotic protection against VOCs-induced developmental toxicity, suggesting its potential as a novel therapeutic intervention for pollution-related developmental disorders.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110250"},"PeriodicalIF":3.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144255311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and histopathological response of non-target organisms to emamectin benzoate: an effective innovative insecticide or a new ecosystem health hazard?","authors":"Meryem Bayrak ,&nbsp;Pınar Arslan Yüce ,&nbsp;Aysel Çağlan Günal ,&nbsp;Göktuğ Gül ,&nbsp;Cristiana Roberta Multisanti ,&nbsp;Caterina Faggio","doi":"10.1016/j.cbpc.2025.110241","DOIUrl":"10.1016/j.cbpc.2025.110241","url":null,"abstract":"<div><div>Emamectin benzoate (EB) is a macrocyclic lactone insecticide, belonging to the avermectins class and ivermectin family and is produced by <em>Streptomyces avermitilis</em>. EB is widely used in agriculture and aquaculture to reduce the damaging effects of pests. This study aimed to investigate the 1) acute toxicity (median lethal concentration values, LC₅₀) and 2) sublethal toxicity (immunological, biochemical and histopathological effects of sublethal concentrations) of EB on non-target freshwater mussels (<em>Unio delicatus</em>). The 96-h LC₅₀ value of EB for <em>U. delicatus</em> was determined as 0.21 mg/L. <em>U. delicatus</em> individuals were exposed to sublethal concentrations at ¹/₁₀ and ¹/₁₀₀ of the LC₅₀ value for 48 h, 7d and 21d. Acute exposure to sub-lethal concentrations resulted in a significant decrease in the total haemocyte count compared to the control groups (<em>p</em> &lt; 0.05). Changes in total antioxidant status and total oxidant levels occurred in the haemolymph. While changes in gill and digestive gland glutathione and advanced oxidation protein products were not significant during acute (48 h and 7d) and chronic (21d) exposure to sub-lethal concentrations of EB, significant increases or decreases were observed in malondialdehyde, compared to control groups (<em>p</em> &lt; 0.05). In addition to immunological and biochemical changes, histopathological changes were observed in gill and digestive gland tissues. The results of this study showed that exposure to EB at two sublethal concentrations in <em>U. delicatus</em> caused biochemical and histopathological changes in the tissues of the haemolymph, gills and digestive glands. The overall results of this study showed that EB is toxic to mussels even at sub-lethal concentrations in acute and chronic exposure.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110241"},"PeriodicalIF":3.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic toxicity of antibiotics and global warming in Danio rerio: Biomarker responses and toxicological effects","authors":"Bárbara S. Diogo ,&nbsp;Sara Rodrigues ,&nbsp;Bent Speksnijder ,&nbsp;Oksana Golovko ,&nbsp;Sara C. Antunes","doi":"10.1016/j.cbpc.2025.110240","DOIUrl":"10.1016/j.cbpc.2025.110240","url":null,"abstract":"<div><div>The combined influence of multiple stress factors on natural ecosystems is a critical concern, as neglecting their effects could compromise essential biological functions. However, limited studies have explored the combined effects of antibiotics and global warming on aquatic ecosystems, leaving a gap in understanding their interaction. This study aimed to assess the toxicity of environmentally relevant concentrations of sulfamethoxazole (SMX: 150 μg/L), trimethoprim (TRIM: 30 μg/L), and their mixture (MIX: 150 μg SMX/L + 30 μg TRIM/L) on <em>Danio rerio</em> at three temperature conditions: standard (26 °C), moderately high (28 °C), and high (32 °C) temperatures. A multi-biomarker approach was used to evaluate the organism's biological status (e.g., antioxidant/detoxification defense enzymes, lipid peroxidation, cholinergic neurotransmission, energetic metabolism, DNA damage). Results indicated that rising temperatures influenced the toxicity level of each antibiotic differently to <em>D. rerio</em>. At 26 °C, all the antibiotics were marginally toxic, and major alterations were observed (oxidative stress and neurotoxicity). Increasing temperature to 28 °C, the toxicity increased, with SMX and MIX exhibiting moderate toxicity, and severe alterations (neurotoxicity and DNA damage). In contrast, TRIM showed only slight toxicity and recorded negligible alterations (antioxidant defense alterations). At higher temperature (32 °C) individual antibiotics revealed slightly toxic with negligible alterations. However, MIX at 32 °C was more toxic, and severe damage was observed (e.g., higher DNA damage). These findings reveal a pressing and alarming threat: combined contaminants impact and climate change could drive aquatic ecosystems toward collapse. Understanding how these stressors interact is critical to preventing potentially irreversible damage to aquatic life.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110240"},"PeriodicalIF":3.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicological impacts of fipronil sulfone: Developmental and behavioral disruptions in zebrafish embryos and larvae","authors":"Bianca de Arruda Leite ,&nbsp;Bruno Rossato ,&nbsp;Carlos Gravato ,&nbsp;Daniel Junqueira Dorta ,&nbsp;Danielle Palma de Oliveira","doi":"10.1016/j.cbpc.2025.110236","DOIUrl":"10.1016/j.cbpc.2025.110236","url":null,"abstract":"<div><div>Fipronil sulfone is a persistent oxidative metabolite of the insecticide fipronil, frequently detected in aquatic systems and known to exert neurotoxic effects on non-target organisms. Despite its environmental relevance, the developmental impacts of fipronil sulfone remain underexplored. In this study, zebrafish embryos and larvae were exposed to environmentally relevant concentrations of fipronil sulfone (12.5–1000 μg/L) at different developmental stages (24, 48, 72, 120, and 144 hpf). Morphological, behavioral, and biochemical endpoints were assessed. Exposure to concentrations ≥125 μg/L significantly reduced swim bladder inflation (up to 100 % inhibition at ≥250 μg/L) and induced spinal curvature in over 60 % of larvae. Survival decreased progressively with increasing concentrations, reaching 100 % mortality at 1000 μg/L. Larvae exposed to 125 μg/L also exhibited hyperactivity followed by reduced locomotion, likely due to blocked GABAergic receptors and acetylcholinesterase inhibition. Moreover, oxidative stress was confirmed by increased reactive oxygen species and glutathione S-transferase activity. These findings demonstrate that even low concentrations of fipronil sulfone can compromise neurodevelopment and survival in zebrafish, underscoring the need for its inclusion in environmental monitoring and risk assessment frameworks.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110236"},"PeriodicalIF":3.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}