Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"Polystyrene nanoplastics cause developmental abnormalities, oxidative damage and immune toxicity in early zebrafish development","authors":"Febriyansyah Saputra ,&nbsp;Azzah Dyah Pramata ,&nbsp;Agoes Soegianto ,&nbsp;Shao-Yang Hu","doi":"10.1016/j.cbpc.2025.110216","DOIUrl":"10.1016/j.cbpc.2025.110216","url":null,"abstract":"<div><div>Aquatic organisms are increasingly affected by polystyrene nanoplastics (PSNPs), which have the potential to disrupt development, induce oxidative stress, and impair immune function. This study examined the effects of PSNPs on zebrafish (<em>Danio rerio</em>) embryos exposed to 0.01, 0.1, 1, and 10 μg/mL from 2 to 120 h post-fertilization (hpf). The findings indicated that developmental abnormalities occurred in a dose-dependent manner, including delayed hatching, decreased survival rates, reduced body length, smaller eye diameter, and enlarged yolk sac area. PSNPs accumulated in the chorion and yolk sac as early as 6 hpf and were detected in vital tissues, such as the eyes, heart, yolk sac, liver, pancreas, intestine, neuromasts, and tail, immediately after hatching. By 120 hpf, PSNPs significantly reduced swimming distance and velocity. Exposure to PSNPs induced oxidative damage evidenced by altered expression of antioxidant-related genes (<em>CAT1, GPX1A, SOD1, NRF2, KEAP1, HSP70, MT</em>), disrupting cellular homeostasis and causing structural and organ defects. Immune toxicity was marked by dysregulated expression of immune-related genes (<em>IL-1β, IL-6, NF-κB, TNF-α, C3B, TLR-1, TLR-3, TLR-4</em>), indicating inflammation and innate immune activation triggered by oxidative damage. This study highlights the interconnected impacts of developmental abnormalities, oxidative damage, and immune toxicity caused by PSNPs exposure, highlighting the systemic impacts of nanoplastics contamination in aquatic environments.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110216"},"PeriodicalIF":3.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The correlation of immunotoxicity with size and chemical properties of particulate matter within macrophages","authors":"Hsiang-Yu Kung ,&nbsp;Yi-Shiuan Wang ,&nbsp;Yung-Jui Chen ,&nbsp;Ting-Hsuan Wu ,&nbsp;Jai-Yu Chen ,&nbsp;Yueh-Feng Wen ,&nbsp;Yu-Cheng Chen ,&nbsp;Hong-Lin Chan ,&nbsp;Hsiu-Chuan Chou","doi":"10.1016/j.cbpc.2025.110215","DOIUrl":"10.1016/j.cbpc.2025.110215","url":null,"abstract":"<div><div>In recent years, air pollution has become a major public health concern. The emission of greenhouse gases aggravates climate change, thereby affecting air quality. Among these pollutants, particulate matter (PM) can cause respiratory and cardiovascular damage. The smaller the particulate matter, the more likely it is to enter the body. However, research on immune responses to PM is limited and many molecular mechanisms remain unknown. Two sizes of particulate matter, PM₄ (&lt;4 μm) and PM₁₀ (&lt;10 μm), were used in this paper. The results showed that the smaller particles tend to adsorb more complex elements. PM₄ caused more severe cell count reduction and appearance changes. Supernatant test with DMSO and PBS suggested a stronger effect of PM size on cells. PM4 exhibited greater toxicity than PM₁₀, as indicated by lower cell survival rates at both 24 and 48 h. Additionally, PM₄ induced higher level of chemotaxis and reactive oxygen species (ROS) production, suggesting a stronger immune activation response. The difference is that the cell cycle arrest of the two cells was at different phases, RAW264.7 arrest at the Sub-G1 phase and J774A.1 arrest at the G2/M phase, which might be due to the differences in the mechanisms of different macrophage strains or the complex composition of PMs triggering multiple cellular mechanisms. Macrophages also produced chemotactic properties and ROS due to the chemical composition of PM. The results indicate that PM affects the immune system by triggering inflammation, oxidative stress, and cell cycle arrest, leading to immune dysfunction.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110215"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From metabolism to lifespan trade-offs: polyethylene microplastics induce circadian disruption and sex-specific aging in Drosophila melanogaster","authors":"Chengpeng Wang,&nbsp;Jie Shen","doi":"10.1016/j.cbpc.2025.110214","DOIUrl":"10.1016/j.cbpc.2025.110214","url":null,"abstract":"<div><div>Microplastics (MPs), particularly polyethylene microplastics (PE-MPs), are increasingly recognized as contaminants in both aquatic and terrestrial environments. However, the ecological impacts of PE-MPs on terrestrial organisms remain underexplored. This study investigates the physiological and behavioral effects of PE-MPs exposure in the terrestrial model organism <em>Drosophila melanogaster</em>, shedding light on the potential risks posed by PE-MPs in land-based ecosystems. After exposing the fruit flies to different concentrations of PE-MPs for 20 days, we assessed several physiological biomarkers, including spontaneous behavioral activity, starvation resistance, metabolic biomarkers, and lifespan. Our findings indicate that PE-MPs exposure significantly affects fruit fly physiology, with increased spontaneous activity, decreased starvation resistance, and reduced triglyceride (TG) and protein levels (in males), reflecting disruption of metabolic processes. While PE-MPs did not affect female reproductive capacity, they did result in sex-specific impacts on lifespan, with male fruit flies showing a significant reduction in both mean and median lifespan at higher PE-MPs concentrations. These results highlight the need to consider the sex-dependent nature of PE-MPs toxicity when assessing their ecological risks. This study contributes new insights into the potential for PE-MPs to disrupt terrestrial ecosystems and underscores the importance of investigating the effects of microplastics on terrestrial invertebrates, providing a foundation for future ecotoxicological research.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110214"},"PeriodicalIF":3.9,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of the ferroptosis pathway in the toxic mechanism of TCDD-induced liver damage in zebrafish","authors":"Yunqi Gao ,&nbsp;Hongsong Chen ,&nbsp;Hua Lian ,&nbsp;Xiaoxu Cai ,&nbsp;Lingtian Xie ,&nbsp;R.G. Ahmed ,&nbsp;Xuguang Lin ,&nbsp;Hongxing Chen ,&nbsp;Wu Dong","doi":"10.1016/j.cbpc.2025.110213","DOIUrl":"10.1016/j.cbpc.2025.110213","url":null,"abstract":"<div><div>Dioxins, especially 2,3,7,8-Tetrachlorodibenzo-<em>p</em>-dioxin (TCDD) are harmful environmental pollutants, which is known to accumulate in humans and animals through ingestion, drinking water, and direct contact, leading to severe liver steatosis and cell death. This study used zebrafish as an experimental model to explore whether TCDD affects the liver via the ferroptosis pathway. The study examined microscopic and ultrastructural characteristics, oxidative stress-related indicators, iron content, and related gene and protein expression in zebrafish larvae liver cells. The results showed that TCDD exposure led to a decrease in the number of hepatocytes in zebrafish larvae. However, pretreatment with the ferroptosis inhibitor ferrostatin-1 (fer-1) alleviated these TCDD-induced changes. The transmission electron microscopy showed that TCDD exposure led to mitochondrial damage in the liver cells, an elevated iron content, a decrease in the level of the ferroptosis-related enzyme glutathione, increased alanine aminotransferase and malondialdehyde enzyme activities, and decreased glutathione peroxidase 4 protein levels. These effects were alleviated by the fer-1 pretreatment. At the gene level, TCDD exposure induced the expression of ferroptosis-related genes (<em>tf, tfr, tfr1b,</em> and <em>fpn</em>), inflammatory factor-related genes (<em>NF-kB, ptgs2a,</em> and <em>ptgs2b</em>), and lipid degeneration and autophagy genes (<em>atg5, ncoa4,</em> and <em>acox1</em>), and inhibited the expression of oxidative stress-related genes (<em>gpx4, slc7a11,</em> and <em>nrf2</em>). The fer-1 pretreatment counteracted these gene expression changes induced by TCDD. These findings indicate that TCDD-induced liver fatty degeneration and cell death are closely related to the ferroptosis pathway.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110213"},"PeriodicalIF":3.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating insecticide resistance in eggplant fruit and shoot borer: Multi-class insecticides and detoxification gene expression","authors":"Kannidi Siddhartha ,&nbsp;Kuttananjan Ragumoorthi ,&nbsp;Venkatasamy Balasubramani ,&nbsp;Sendha Venkatachary Krishnamoorthy ,&nbsp;Thiruvenkatasamy Saraswathi ,&nbsp;Ettiappan Sumathi","doi":"10.1016/j.cbpc.2025.110210","DOIUrl":"10.1016/j.cbpc.2025.110210","url":null,"abstract":"<div><div>Eggplant fruit and shoot borer, <em>Leucinodes orbonalis</em> (Lepidoptera: Crambidae), is a destructive borer pest that infests eggplant crop from nursery stage to fruiting. This study aimed to evaluate the current insecticide resistance status of <em>L. orbonalis</em> collected from ten different locations of Tamil Nadu, India, using a fruit dip bioassay. Late second instar larvae were exposed to spinosad, spinetoram, thiacloprid and emamectin benzoate. Median lethal concentration (LC₅₀) and resistance ratios (RR) were estimated for each population using a susceptible NBAIR population as a reference. The results showed that emamectin benzoate was highly toxic to <em>L. orbonalis</em>. All field populations exhibited a low to high level of resistance against spinosad (7.4 to 72.1 fold) and spinetoram (6.6 to 67.8 fold), while susceptibility to low levels of resistance was observed against thiacloprid (0.89 to 5.06 fold) and emamectin benzoate (1.48 to 11.29 fold). Additionally, detoxification enzyme assays were conducted for cytochrome P450 monooxygenases (CYP/MFO), glutathione S-transferases (GST) and carboxylesterases (CarE) revealing high specific activity in the Dharmapuri (DMP) population, correlating with resistance. Furthermore, quantitative real-time PCR analysis was conducted to assess detoxification gene expression in resistant (DMP) and susceptible populations after insecticide exposure, revealing significant overexpression of <em>cyp306a1</em> and <em>ce125</em> across the studied insecticides, particularly after 48 h, indicating their key role in resistance. These findings highlight the need for insecticide rotation with different modes of action to mitigate resistance development in <em>L. orbonalis</em>, providing valuable insights for sustainable pest management in Tamil Nadu and similar agricultural contexts worldwide.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110210"},"PeriodicalIF":3.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic understanding of the toxic effects of tri-n-butyl phosphate (TnBP) and tricresyl phosphate (TCP) to Escherichia coli: Evidence from alterations in biomarker expression and perturbations of the metabolic network","authors":"Xiaolong Yu ,&nbsp;Runlin Yao ,&nbsp;Ruipu Yao ,&nbsp;Xu Jin ,&nbsp;Jiahui Huang ,&nbsp;Qianwei Liang ,&nbsp;Ling N. Jin ,&nbsp;Jianteng Sun","doi":"10.1016/j.cbpc.2025.110211","DOIUrl":"10.1016/j.cbpc.2025.110211","url":null,"abstract":"<div><div>Tri-n-butyl phosphate (TnBP) and tricresyl phosphate (TCP), emerging flame retardants and plasticizers, have garnered increasing attention due to their potential risks to ecosystem. A few researches regarding the toxicological mechanisms of TnBP and TCP had been performed, while molecular-level toxic effects of them and metabolic response using microbial models are the lack of relevant investigation. Thus, we investigated the cytotoxicity, oxidative stress response, and metabolic response in <em>E. coli</em> exposed to TnBP and TCP. Exposure to them significantly increased the activities of antioxidant enzymes, indicating activation of the antioxidant defense system. Excessive accumulation of reactive oxygen species (ROS) triggered various biological events, including a reduction in membrane potential (MP), decrease of adenosine triphosphatase (ATPase) activity, and increased malondialdehyde (MDA) content. These findings suggested that oxidative damage compromised membrane proteins function, membrane stability, and intracellular homeostasis. GC–MS and LC-MS-based metabolomics analyses revealed that TnBP and TCP strongly disrupted multiple metabolic pathways, including carbohydrate metabolism, nucleotide metabolism, lipid metabolism, beta-alanine metabolism, pyruvate metabolism and oxidative phosphorylation. These disruptions highlighted the inhibitory effects on molecular functions and metabolic processes. Notably, lipids biomarkers e.g., PC(11:0/16:0), PA(17:1(9Z)/18:2(9Z,12Z)), PE(17:0/14:1(9Z)), and LysoPE(0:0/18:1(11Z)) were significantly altered, verifying that the regulation of lipid-associated metabolite synthesis plays a protective role in maintaining cellular membrane function. In summary, this study enhances our understanding of TnBP and TCP toxicity in <em>E. coli</em>, providing novel insights into their toxicological mechanisms at molecular and network levels. These findings underscore the ecological risks posed by organophosphate flame retardants in aquatic ecosystem.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110211"},"PeriodicalIF":3.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, biochemical, and histological alterations in juvenile giant freshwater prawns (Macrobrachium rosenbergii) exposed to sub-lethal concentrations of 2,4-D dimethylammonium","authors":"Sugunya Kumla ,&nbsp;Boonthiwa Chartchumni ,&nbsp;Phochit Nanthanawat ,&nbsp;Jakkaphun Nanuam ,&nbsp;Sawipa Ruttanakorn ,&nbsp;Panomsak Meemon ,&nbsp;Chutima Thanomsit","doi":"10.1016/j.cbpc.2025.110212","DOIUrl":"10.1016/j.cbpc.2025.110212","url":null,"abstract":"<div><div>The widespread use of herbicides, such as 2,4-D dimethylammonium, poses significant risks to aquatic ecosystems. This study assessed the toxicological effects of 2,4-D dimethylammonium on juvenile giant freshwater prawns (<em>Macrobrachium rosenbergii</em>) by assessing mortality, total hemocyte count (THC), acetylcholinesterase (AChE) expression, and tissue integrity using optical coherence tomography (OCT) and histological analysis. Prawns were exposed to 0, 250, 300, 350, 400, and 450 μL/L of 2,4-D dimethylammonium for 24, 48, 72, and 96 h, and both LC₅₀ values and sub-lethal effects were examined. Acute toxicity tests revealed dose- and time-dependent mortality, with declining LC₅₀ values over 24 to 96 h. THC levels significantly decreased (<em>p</em> &lt; 0.05) at higher concentrations, with prawns exposed to 450 μL/L showing a 35 % reduction at 96 h. Western blot analysis revealed AChE suppression, with expression becoming faintly detectable at 72 h and undetectable at 96 h at 450 μL/L. OCT analysis showed tissue disorganization, increased light scattering, and structural degradation, aligning with histological findings of hepatopancreas and muscle deterioration in high-exposure groups. The study highlights the environmental risks of 2,4-D dimethylammonium contamination and supports OCT as a non-invasive tool for assessing pesticide toxicity in aquatic organisms.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110212"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acute exposure to azoxystrobin on embryos and juveniles of the freshwater snail Lymnaea stagnalis","authors":"Suleyman Ilhan ,&nbsp;Sezgi Somuncu ,&nbsp;Harika Atmaca","doi":"10.1016/j.cbpc.2025.110209","DOIUrl":"10.1016/j.cbpc.2025.110209","url":null,"abstract":"<div><div>Azoxystrobin (AZ) is a systemic fungicide applied to soil to control a wide range of plant pathogenic fungi. AZ residues have been detected in surface waters due to excessive release, posing a risk to aquatic organisms. This study evaluated the toxic effects of short-term AZ exposure on embryos and juveniles of <em>Lymnaea stagnalis</em>. First, the 96-h LC₅₀ values of AZ were estimated as 7.98 (6.50–9.85) mg/L for embryos and 2.90 (2.30–3.86) mg/L for juveniles, indicating that juveniles are more sensitive to acute AZ toxicity. Additional experiments were conducted to assess the sublethal effects of AZ on embryos and juveniles. The highest sublethal exposure concentrations were set at approximately 20 % of the respective estimated LC₅₀ values for embryos and juveniles, while the lower concentrations were determined by successive ten-fold dilutions to include environmentally relevant AZ levels. Accordingly, embryos were exposed to 0, 15, 150, and 1500 μg/L, whereas juveniles were exposed to 0, 5, 50, and 500 μg/L over a 96-h period. In embryos, AZ exposure led to developmental delays, reduced growth, decreased hatching success and mortality at 1500 μg/L. Likewise, juvenile snails displayed marked biochemical and molecular alterations across all tested concentrations. AZ-induced ROS generation and elevated MDA levels were observed, alongside increased activities of SOD, CAT, GPx, and GR. Additionally, mRNA transcripts of HSP40, HSP70, SOD, CAT, GPx, and GR were upregulated in juveniles, even at the lowest exposure concentration. In conclusion, AZ contamination poses a significant ecological risk to the early life stages of freshwater gastropods.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110209"},"PeriodicalIF":3.9,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurotoxicity induced by difenoconazole in zebrafish larvae via activating oxidative stress and the protective role of resveratrol","authors":"Chunlan Liu ,&nbsp;Jiansheng Zhu ,&nbsp;Renfei Zhu ,&nbsp;Yifei Yin","doi":"10.1016/j.cbpc.2025.110208","DOIUrl":"10.1016/j.cbpc.2025.110208","url":null,"abstract":"<div><div>Difenoconazole (DIF) is a typical triazole fungicide detected in the aquatic ecosystem and organisms. However, the neurotoxic effects of DIF remain largely unknown. This study aimed to investigate the neurotoxicity of DIF in zebrafish and the underlying neuroprotective properties of resveratrol (RES, an antioxidant polyphenol). Zebrafish embryos/larvae were treated with 0.6 and 1.2 mg/L DIF from 4 to 96 h post fertilization (hpf) and neurodevelopment was systematically assessed. DIF induced developmental toxicity and aberrant neurobehaviors, including decreased movement time, swimming distance and clockwise rotation times. DIF suppressed the neurogenesis of the central nervous system (CNS) in <em>HuC:egfp</em> transgenic zebrafish and the length of motor neuron axon in <em>hb9:egfp</em> transgenic zebrafish. DIF inhibited cholinesterase activities and downregulated neurodevelopment related genes. DIF also increased oxidative stress via excessive production of reactive oxygen species and decreased activities of antioxidant enzymes, subsequently triggering neuronal apoptosis in the brain. RES partially reinstated DIF-induced neurotoxicity and developmental toxicity by inhibiting excessive oxidative stress and apoptosis, suggesting the involvement of oxidative stress in DIF-induced neurotoxicity. Overall, this study identified the potential mechanisms underlying DIF-induced neurotoxicity and suggested RES as a promising therapeutic strategy.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"295 ","pages":"Article 110208"},"PeriodicalIF":3.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing neonicotinoid pollution in aquatic ecosystems: A systematic review and bibliometric-content analysis","authors":"Qinglin Yang ,&nbsp;Xiaoqi Tang ,&nbsp;Yuzhuo He ,&nbsp;Xianyun Wu ,&nbsp;Xiaobo Yu ,&nbsp;Yanhong Li ,&nbsp;Zhengli Wu","doi":"10.1016/j.cbpc.2025.110207","DOIUrl":"10.1016/j.cbpc.2025.110207","url":null,"abstract":"<div><div>The widespread use and distribution of neonicotinoids (NNIs) have led to their significant accumulation in aquatic ecosystems, posing serious ecological risks to non-target species and the human food chain. This review employed bibliometric analysis to examine global research from 2013 to 2023, highlighting key trends, advancements, and research priorities. Moreover, we summarized the global distribution of NNIs in various aquatic environments through content analysis and assessed their ecotoxicological effects under controlled laboratory conditions. Our findings indicate a growing global concern about NNIs in aquatic systems, with research efforts primarily concentrated in regions most affected by their use. The presence of NNIs across different water bodies highlights widespread contamination, with China facing particularly severe pollution. However, research on the safety of NNIs in aquatic environments remains insufficient. Future studies should focus on monitoring chronic NNIs exposure and its long-term ecological impacts through field research. Moreover, developing microbial formulations, integrating phytoremediation, and combining multiple technologies for synergy are crucial for developing sustainable strategies to mitigate NNIs pollution, protect human health, and preserve aquatic ecosystems.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"294 ","pages":"Article 110207"},"PeriodicalIF":3.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}