Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"Effects of short-term exposure to environmentally relevant pesticides mixture on morphological alterations, oxidative-nitrative stress biomarkers, cellular apoptosis, and antioxidant expression in kidneys of goldfish","authors":"Esmirna Cantu ,&nbsp;Md Saydur Rahman","doi":"10.1016/j.cbpc.2025.110337","DOIUrl":"10.1016/j.cbpc.2025.110337","url":null,"abstract":"<div><div>Chemical stressors are pervasive, affecting both terrestrial and aquatic environments. The continual influx of these toxins is damaging ecosystems and the organisms that inhabit them. The abundance of environmental toxins makes aquatic habitats inhospitable for aquatic life. These chemical stressors consistently disrupt the life processes of aquatic organisms, particularly their physiological functions. This study examined on the effects of environmentally relevant pesticides mixture (low-dose and high-dose: S-metolachlor: 2.4 go/L, 12 μg/L; linuron: 2.0 μg/L, 10 μg/L; isoproturon: 1.2 μg/L, 6.0 μg/L; tebucanozole: 1.2 μg/L, 6.0 μg/L; aclonifen: 0.8 μg/L, 4.0 μg/L; atrazine: 0.4 μg/L, 2.0 μg/L; pendimethalin: 0.4 μg/L, 2.0 μg/L, and azinphos-methyl: 0.8 μg/L, 4.0 μg/L) on tissue morphology, cellular apoptosis, and nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), renin, superoxide dismutase (SOD), and catalase (CAT) expression in the kidneys of goldfish (<em>Carassius auratus</em>) exposed for one week under controlled laboratory conditions. Histopathological analysis revealed severe tissue damage in the kidneys, while silver staining identified melano-macrophage centers, and immunohistochemistry and real-time PCR shed light on the expression of molecular biomarkers in these tissues. Exposure to pesticide mixtures caused fish to exhibit glomerular shrinkage, enlargement of Bowman's space, and degradation of glomerular cells. Moreover, the expression of renin, DNP, NTP, SOD, and apoptotic nuclei increased in kidney tissues, while CAT expression decreased. Overall, our findings indicate that exposure of goldfish to an environmentally relevant pesticide mixture leads to increased cellular damage and altered osmoregulatory and antioxidant enzyme expressions, which may impair physiological functions, including growth, reproduction, and development in teleost fish.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110337"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective effect of ferulic acid in valproic acid induced autism like behaviour in zebrafish via modulation of PI3K/AKT/mTOR pathway","authors":"Dhrita Chatterjee ,&nbsp;Shamsher Singh","doi":"10.1016/j.cbpc.2025.110347","DOIUrl":"10.1016/j.cbpc.2025.110347","url":null,"abstract":"<div><div>Valproic acid (VPA), a widely used antiepileptic and mood stabilizing drug, is known to induce autism-like features when administered during neurodevelopment. Recent evidence suggests that VPA exposure during adulthood may also elicit autism spectrum disorder (ASD)-like features by altering key signalling pathways, such as phosphoinositide 3-kinase/AKT/mammalian target of rapamycin (PI3K-AKT-mTOR), and cause behavioural and neuromorphological deficits. The study explored the neuroprotective properties of ferulic acid (FA) in VPA-induced cognitive and behavioural impairments. Zebrafish were exposed to VPA at 500 μM for four consecutive days to induce ASD-like features. After 4 days of VPA exposure, they were treated with FA (50, 100, and 200 mg/kg) and risperidone (0.5 mg/kg) for 4 days. Behavioural (T-maze, Novel Tank Driving Test (NTDT), and social interaction), biochemical (oxidative markers), molecular changes (PI3K, mTOR by ELISA, and AKT by immunohistochemistry), and histopathological analyses were performed to confirm the neuroprotective properties of ferulic acid (FA). VPA (500 μM) exposure significantly deteriorated behavioural and molecular alteration levels (<em>p</em> &lt; 0.001 vs. normal control group) in zebrafish. However, FA (100 and 200 mg/kg) significantly improved cognitive and behavioural alterations, as well as oxidative marker and neurotransmitter levels (<em>p</em> &lt; 0.05 vs. VPA group) in zebrafish. Treatment also improved histopathological changes and AKT levels (<em>p</em> &lt; 0.001 vs. the VPA group) in zebrafish. Our results demonstrated that the therapeutic effect of FA in VPA induced autism like symptoms in zebrafish was mediated by its antioxidant, anti-inflammatory, and anti-apoptotic properties through modulation of the PI3K-AKT-mTOR pathway, offering a promising therapeutic strategy for ASD-like symptoms.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110347"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of cypermethrin on the clams Ruditapes philippinarum (A. Adams & Reeve, 1850)","authors":"Lihua Qi ,&nbsp;Chengang Hua ,&nbsp;Zongqi Ye ,&nbsp;Xu Shen ,&nbsp;Yuefeng Cai ,&nbsp;Xin Shen","doi":"10.1016/j.cbpc.2025.110366","DOIUrl":"10.1016/j.cbpc.2025.110366","url":null,"abstract":"<div><div>Cypermethrin, highly toxic to aquatic life, impacts <em>Ruditapes philippinarum</em> (A. Adams &amp; Reeve, 1850), an economically valuable species. Thus, studying its toxicity mechanism to <em>R. philippinarum</em> is crucial. In this research, <em>R. philippinarum</em> was exposed to 5000 ng/L cypermethrin and sampled on days 0, 1, 3, 6, 10, 15, and 21 to assess enzymatic activities and conduct transcriptome sequencing. The enzymatic activity results showed that cypermethrin could increase the activity of the ethoxyresorufin-O-deethylase (EROD), turn on the antioxidant defenses, cause an initial increase in total antioxidant capacity (T-AOC) and then a decrease, increase the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), and inhibit superoxide dismutase (SOD). Furthermore, the contents of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) increased in the body at 10, 15, and 21 days of exposure. Transcriptome sequencing was carried out to analyze the responses of cypermethrin stress for 1 day and 21 days. Differentially expressed genes (DEGs) were linked to detoxification metabolism and oxidative stress, according to Gene Ontology (GO) enrichment analysis. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis demonstrated that cypermethrin had toxic effects through key factors affecting detoxification metabolism, oxidative stress, immune response, and apoptosis-related pathways. This study gives insights into the harmful mechanism of cypermethrin stress on <em>R. philippinarum</em>, as well as a theoretical basis for assessing the ecological danger of cypermethrin in Chinese coastal waters.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110366"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling excessive feed-sources copper-induced ileitis in chickens: Insights into tight junction damage and ROS/NLRP3/pyroptosis axis","authors":"Boran Zhou ,&nbsp;Yufei Cao ,&nbsp;Yingxue Zhang,&nbsp;Mingwei Xing,&nbsp;Yu Wang","doi":"10.1016/j.cbpc.2025.110357","DOIUrl":"10.1016/j.cbpc.2025.110357","url":null,"abstract":"<div><div>Copper, widely used as a growth promoter and antibacterial agent, is commonly added to livestock and poultry feed. However, this widespread use leads to its accumulation in the animals' bodies, resulting in intestinal toxicity. The specific mechanisms of copper-induced ileitis in broilers remain unclear. In this study, broilers were fed diets containing 0, 100, or 300 mg/kg CuSO₄ over a five-week period. Results showed that the high‑copper group (300 mg/kg) exhibited significant suppression of antioxidant defenses, including reduced heme oxygenase-1 (HO-1), NAD(<em>P</em>)H quinone dehydrogenase 1 (NQO1), total superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels, while catalase (CAT) activity was paradoxically elevated. Notably, NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome complex activation (NLRP3, apoptosis-associated speck-like protein containing a CARD [ASC], Caspase-1) and upregulated pyroptosis markers (Caspase-1, interleukin-1β [IL-1β]) indicated reactive oxygen species (ROS)–NLRP3 axis involvement. Concurrently, pro-inflammatory mediators (interleukin-7 [IL-7], interleukin-17 [IL-17], inducible nitric oxide synthase [iNOS], tumor necrosis factor-α [TNF-α]) were dysregulated, accompanied by diminished expression of tight junction proteins (zonula occludens-1 [ZO-1], Claudin-3, Occludin) and inhibition of the Wnt/β-catenin pathway. These findings demonstrate that copper-induced intestinal inflammation and pyroptosis are driven by ROS–NLRP3 axis activation, while simultaneously compromising mucosal barrier integrity. This study elucidates critical mechanisms of copper toxicity in broilers and highlights risks associated with excessive copper exposure.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110357"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The plastic threat to marine gastropods: Oxidative stress, energy metabolism and impaired recovery in juvenile abalone (Haliotis discus hannai) exposed to polystyrene microplastics","authors":"Chaoyi Xie ,&nbsp;Hongce Song ,&nbsp;Junjie Li ,&nbsp;Shuai Xu ,&nbsp;Liqun Ren ,&nbsp;Chunnuan Zhao ,&nbsp;Yuping Wu ,&nbsp;Xiaotong Wang ,&nbsp;Biao Wu ,&nbsp;Shubao Zhang ,&nbsp;Haiying Han ,&nbsp;Tao Yu","doi":"10.1016/j.cbpc.2025.110361","DOIUrl":"10.1016/j.cbpc.2025.110361","url":null,"abstract":"<div><div>Microplastic (MP) pollution challenges marine ecosystems, with gastropods like <em>Haliotis discus hannai</em> being potentially vulnerable. However, the reversibility of MP-induced physiological damage in gastropods remains poorly understood. This study investigated the effects of polystyrene microplastics (PS-MPs; 0, 100, and 1000 μg/L) on <em>H. discus hannai</em> in a 14-day exposure followed by a 7-day recovery experimental design. The analysis focused on antioxidant status, energy metabolism, and immune pathways. Results showed dose-dependent oxidative stress during PS-MPs exposure, indicated by significantly (<em>P</em> &lt; 0.05) elevated antioxidant enzyme activities (Superoxide dismutase, Catalase and Glutathione peroxidase), which decreased but did not normalize during recovery. Exposure to a low concentration of PS-MPs (100 μg/L) resulted in metabolic adjustments consistent with homeostatic maintenance, whereas a high concentration (1000 μg/L) significantly increased lactate dehydrogenase and Na⁺/K⁺-ATPase activities, suggesting metabolic disruption. These energy metabolism alterations persisted, showing incomplete recovery. Immune pathway analysis revealed minimal changes at low PS-MPs concentrations but significant enrichment of pathways like IL-17 and Fc-Epsilon-RI signaling at high concentrations during exposure. Notably, sustained activation of immune pathways, including NOD-like receptor, Toll-like receptor, and IL-17 signaling (particularly in the high-concentration group), persisted during recovery, potentially indicating prolonged cellular stress or links to apoptosis. Furthermore, Integrated Biomarker Response (IBR) analysis confirmed that <em>H. discus hannai</em> remained severely impacted even after the recovery period. This study provides crucial evidence on the complex toxicodynamics of PS-MPs in <em>H. discus hannai</em>, highlighting incomplete physiological recovery and significant damage during the recovery period following exposure, especially at high concentrations.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110361"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish cancer and its assistance to human cancer research: A fresh perspective","authors":"Hao Dan ,&nbsp;Wentong Wang ,&nbsp;Xuhong Chen ,&nbsp;Mariam N. Goda ,&nbsp;Mohamed S. Kisha ,&nbsp;Quanquan Cao","doi":"10.1016/j.cbpc.2025.110369","DOIUrl":"10.1016/j.cbpc.2025.110369","url":null,"abstract":"<div><div>Cancer is a complex disease affecting various species, including fish, which have emerged as valuable models for studying cancer mechanisms. Recent studies highlight the similarities and differences between fish and mammalian tumors, offering insights for novel human cancer therapies. Fish models, particularly zebrafish, replicate key cancer phenotypes and molecular pathways seen in humans, making them powerful tools for cancer research. This review explores the transition from fish to human cancer studies, focusing on the molecular basis, diagnosis, and treatment of fish cancer. Key findings include the role of blood vessel formation in fish tumors and the application of zebrafish tumor models to understand human cancer. These studies provide new perspectives and technologies for human cancer research, though challenges remain in fully leveraging fish models for future therapeutic advancements.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110369"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-23a modulates chlorantraniliprole susceptibility by targeting SfGSTs3 in Spodoptera frugiperda (Smith)","authors":"Fan-Bin Kong ,&nbsp;Yong-Po Lv ,&nbsp;Bai-Zhong Zhang ,&nbsp;Meng-Yuan Zhang ,&nbsp;Ling-Ling Cui ,&nbsp;Ren-Jie Li ,&nbsp;Dong-Mei Chen ,&nbsp;Yu-Yang Peng ,&nbsp;Run-Qiang Liu","doi":"10.1016/j.cbpc.2025.110367","DOIUrl":"10.1016/j.cbpc.2025.110367","url":null,"abstract":"<div><div>The fall armyworm <em>Spodoptera frugiperda</em> (Smith) can damage many crops distributed worldwide, and chemical insecticide application is the main control strategy. However, the frequent application of insecticides can lead to severe insecticide resistance in <em>S. frugiperda</em>. Glutathione S-transferases (GSTs) play a critical role in insecticide resistance in pests. In this study, it was found that the expression of SfGSTs3 was significantly up-regulated after exposure to chlorantraniliprole. After injection of dsSfGSTs3, the susceptibility of chlorantraniliprole was improved, and microRNA-23a binding on 3’UTR of SfGSTs3 was found. Luciferase reporter assays revealed that the effects of miR-23a on <em>SfGSTs3</em> expression were suppressed via this binding site in <em>S. frugiperda</em>. Injection of the miR-23a agomir significantly reduced <em>SfGSTs3</em> expression, together with increased chlorantraniliprole susceptibility. In contrast, injection of the miR-23a antagomir significantly improved <em>SfGSTs3</em> expression and thus decreased chlorantraniliprole susceptibility in the larvae of <em>S. frugiperda</em>. These findings provide a theoretical foundation for better understanding the posttranscriptional regulation of <em>SfGSTs3</em> and can be used to further study the mechanism by which miRNAs regulate insecticide susceptibility in pests.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110367"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How microplastics influence the health and microbiota of aquatic invertebrates: A review","authors":"Haksoo Jeong ,&nbsp;Zhou Yang ,&nbsp;Jae-Seong Lee","doi":"10.1016/j.cbpc.2025.110333","DOIUrl":"10.1016/j.cbpc.2025.110333","url":null,"abstract":"<div><div>Concern about microplastics (MPs) in aquatic ecosystems, and their hazardous effects on aquatic organisms and microbiota in particular, is growing globally. Previous reviews focused on the impact of MPs on biological responses and microbial alterations in fish. The impacts of MPs on other aquatic organisms (mainly invertebrates) remain poorly understood. This review concentrates on the effects of MPs on aquatic organisms other than fish. First, we describe an important microscopic ecosystem associated with MPs and its function in aquatic systems. Second, we elucidate the latest trends in relevant research and experimental designs. We also discuss the toxicity of MPs in various biological and molecular responses, microbial changes, and the relationship between host health and microbiota in aquatic organisms. Last, we examine the combined effects of MPs, climate change, and pollutants on microbiota and hosts. This review provides an overall research background for MPs and microbiota in aquatic ecosystems and suggests future research directions.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110333"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol attenuates trimethyltin chloride-induced cardiac defects via the ROS/Wnt/β-catenin pathway","authors":"Hanwen Hu ,&nbsp;Jin Chen ,&nbsp;Xin Wang ,&nbsp;Mingxue Gong ,&nbsp;Wenzhuo Li ,&nbsp;Yiming Liu ,&nbsp;Hongbiao Li ,&nbsp;Ming Huang ,&nbsp;Anfei Liu ,&nbsp;Zhenzhong Liu","doi":"10.1016/j.cbpc.2025.110373","DOIUrl":"10.1016/j.cbpc.2025.110373","url":null,"abstract":"<div><div>Trimethyltin chloride (TMT), a toxic byproduct of organotin manufacturing, is an emerging environmental contaminant linked to developmental cardiotoxicity. However, its pathogenic mechanism remains undefined. Here, using zebrafish embryos, we show that TMT exposure induced dose-dependent cardiac malformations, increasing pericardial area by 44.9 % and decreasing heart rate by 16.8 % at 5 μM (both <em>P</em> &lt; 0.001), accompanied by excessive ROS (+48.5 %, <em>P</em> &lt; 0.001) and mitochondrial ROS (+82.8 %, <em>P</em> &lt; 0.001) generation. Co-treatment with resveratrol (RSV) or the ROS inhibitor <em>N</em>-acetylcysteine (NAC) reduced TMT-induced cardiac defects and suppressed ROS and mitochondrial ROS overproduction (all <em>P</em> &lt; 0.05). RSV or NAC also mitigated DNA damage, mitochondrial injury, and apoptosis in the heart. Mechanistically, TMT inhibited the Wnt/β-catenin signaling pathway (−61.7 % β-catenin, <em>P</em> &lt; 0.001), an effect attenuated by RSV or NAC. Inhibition of apoptosis with Ac-DEVD-CHO or activation of Wnt/β-catenin signaling with CHIR likewise alleviated TMT-induced cardiac malformations. These results indicate that TMT disrupts heart development through ROS-mediated suppression of Wnt/β-catenin signaling, leading to mitochondrial damage, DNA damage, and apoptosis. Furthermore, RSV, a dietary antioxidant, provides significant protection against TMT-induced cardiac developmental toxicity. This study identifies potential molecular targets for preventing and treating embryonic heart injury caused by environmental toxicants.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110373"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enantioselective toxicity of 3,4-methylenedioxypyrovalerone in Daphnia magna: Assessment of morphophysiological, behavioural, biochemical and reproductive responses","authors":"Ariana Pérez-Pereira ,&nbsp;Ana R. Carvalho ,&nbsp;Pedro A. Costa ,&nbsp;Bruno B. Castro ,&nbsp;João S. Carrola ,&nbsp;Virgínia M.F. Gonçalves ,&nbsp;Ana R.L. Ribeiro ,&nbsp;Ana Sofia Almeida ,&nbsp;Carla Fernandes ,&nbsp;Maria E. Tiritan ,&nbsp;Cláudia Ribeiro","doi":"10.1016/j.cbpc.2025.110375","DOIUrl":"10.1016/j.cbpc.2025.110375","url":null,"abstract":"<div><div>The enantioselective toxicity of 3,4-methylenedioxypyrovalerone (MDPV) remains unexplored in freshwater organisms. This study investigated the effects of MDPV racemate and its enantiomers in <em>Daphnia magna</em> using two approaches: (<em>i</em>) an 8-day sub-chronic assay focused on early development stages; and (<em>ii</em>) the standard 21-day reproduction assay. The sub-chronic assay tested two environmental concentrations (0.10 and 1.0 μg L⁻¹), for both racemate or individual enantiomers, and a higher sublethal concentration of 10 μg L⁻¹ for the racemate. Survival, morphophysiological, behavioural, reproductive, and biochemical responses were evaluated. The reproduction assay tested concentrations from 0.10 to 1.79 μg L⁻¹, and evaluated survival, body size, and reproductive effects.</div><div>Sub-chronic exposure to 10 μg L⁻¹ (<em>R</em>,<em>S</em>)-MDPV decreased survival. MDPV racemate and its enantiomers changed morphophysiological parameters, except for body growth. In juveniles, the heart area increased with 0.10 μg L⁻¹ of (<em>R</em>)-MDPV whereas the heart size decreased with 0.10 and 1.0 μg L⁻¹ of (<em>S</em>)-MDPV. Enantioselective effects were observed in swimming behaviour: (<em>S</em>)-MDPV increased speed and (<em>R</em>)-MDPV increased active time. No effects were observed in biochemical (except in reactive oxygen species for (<em>S</em>)-MDPV) and early reproductive parameters. The reproduction assay revealed enhanced growth in all MDPV forms (except at 0.56 μg L⁻¹). A nonsignificant reduction in survival to the higher concentrations of (<em>R</em>)-MDPV was accompanied by reduced reproductive output (at 1.00 and 1.79 μg L⁻¹) and intrinsic rate of population increase (at 1.79 μg L⁻¹). Our findings suggest that, in general, (<em>S</em>)-MDPV triggers more severe sub-chronic effects, whereas (<em>R</em>)-MDPV is associated with harmful effects for longer time exposure.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"299 ","pages":"Article 110375"},"PeriodicalIF":4.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}