Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"Synergistic toxicity of cadmium and triadimefon on the microbiota and health of Rana dybowskii tadpoles.","authors":"Xiao-Yun Han, Peng Guo, Qiu-Ru Fan, Qing-Bo Zhou, Ming-da Xu, Xin-Zhou Long, Li-Yong Cui, Qing Tong","doi":"10.1016/j.cbpc.2024.110092","DOIUrl":"10.1016/j.cbpc.2024.110092","url":null,"abstract":"<p><p>The skin and gut microbiota are crucial to amphibians. Triadimefon (TF), a widely used triazole fungicide, controls crop diseases and regulates growth, with uncertain effects on amphibian microbiota. Contamination, typically involving mixed chemicals at low concentrations, including cadmium (Cd) and TF, may detrimentally affect amphibian growth, survival, and microbiota health in both the skin and gut, but few research has examined these consequences. This research examines the impact of Cd and TF on Rana dybowskii tadpoles, focusing on survival, body mass, and microbiome changes over 28 days across four groups: control, Cd, TF, and Cd + TF groups. Results showed significant reductions in survival and body mass in Cd and TF-treated groups, with the combination group being the most affected. Microbiota analysis revealed significant dysbiosis in both gut and skin microbiomes under pollutant stress, with a marked microbiota and a shift in dominant microbial communities. Function prediction analysis based on the microbiome composition highlighted significant differences across various biological pathways, including metabolism, immune system, environmental adaptation, and disease resistance. These alterations suggest that pollutant exposure compromises the tadpoles' ability to maintain homeostasis and resist pathogens. In conclusion, this study reveals the detrimental effects of Cd and TF on the survival, growth, and microbiomes of R. dybowskii tadpoles, indicating significant environmental and health risks.</p>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":" ","pages":"110092"},"PeriodicalIF":3.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-adrenergic blockade via atenolol negatively affects body and heart mass and renal morphology in the developing chicken (Gallus Gallus Domesticus).","authors":"Josie J Rossitto Lopez, I I Dane Crossley, Warren W Burggren","doi":"10.1016/j.cbpc.2024.110089","DOIUrl":"10.1016/j.cbpc.2024.110089","url":null,"abstract":"<p><p>Atenolol is a widely prescribed β₁-cardioselective blocker. We studied atenolol effects on cardiac and renal development in day 18 (D18) chicken embryos. Embryos were dosed with atenolol (3 μg atenolol/g estimated embryo mass) for three days during one of the mesonephric kidney stage (D7-D9), mesonephric-metanephric stage (D11-D13), or metanephric stage (D15-D17), and then sampled on D18. Wet embryo body mass in atenolol-treated groups was reduced at D18 (P < 0.01). Wet heart mass of atenolol-treated embryos was significantly (P < 0.01) reduced in the mesonephric stage on D18. Similarly, kidney mass in atenolol-treated mesonephric and metanephric stages was significantly reduced at D18. Nephron density was 40 % lower following atenolol treatment during the mesonephros stage. Individual glomerular areas of mesonephric- and metanephric-treated stages were significantly larger (P < 0.01) than controls, but overall glomerular area was reduced in the meso- and meso-metanephros populations. Collectively, these data suggest that chronic atenolol treatment results in major renal remodeling and that the mesonephros renal stage (D7-D9), is the critical window for effects of atenolol on renal morphology. Acute atenolol application at D11 through D19 had no effect on mean arterial blood pressure or heart rate, even though these variables were acutely altered as early as Day 15 by isoproterenol. Collectively, these data suggest that the morphological effects of atenolol were not the result of altered perfusion. Further experiments are required to determine if reduced embryo, cardiac and renal masses are specific to chronic atenolol treatment, or whether other β blockers might have similar effects.</p>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":" ","pages":"110089"},"PeriodicalIF":3.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early-life exposure to di(2-ethylhexyl) phthalate impairs reproduction in adult female zebrafish (Danio rerio).","authors":"Biljana Tesic, Svetlana Fa Nedeljkovic, Jelena Markovic Filipovic, Dragana Samardzija Nenadov, Kristina Pogrmic-Majkic, Nebojsa Andric","doi":"10.1016/j.cbpc.2024.110090","DOIUrl":"10.1016/j.cbpc.2024.110090","url":null,"abstract":"<p><p>Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer used in various industrial and consumer products. It is not covalently bound within these products and leaches out during repeated use, heating, or cleaning. Main routes of environmental DEHP pollution are through the industrial and municipal wastewaters, which pollute aquatic environments. The reproductive system of adult fish is known to be vulnerable to DEHP exposure; however, the effects of early-life exposure to DEHP on reproductive function in adult zebrafish (Danio rerio) females are less studied. To evaluate the impact of early-life exposure to DEHP on freshwater female fish reproduction, zebrafish embryos were exposed to DEHP at 0, 10, 100, and 1000 nmol/L from 5 h post-fertilization (hpf) to 120 hpf (larval stage) and then raised to adulthood in clean water. DEHP decreased the number of released eggs and the fertilization rate after mating with unexposed males. Bodyweight and length, the weight of the ovaries, and the gonadosomatic index were decreased in adult female zebrafish following early-life exposure to DEHP. Histological analysis of the ovaries revealed that DEHP inhibited oogenesis. Serum 17β-estradiol levels were significantly reduced. DEHP inhibited gene expression of all three nuclear estrogen receptors in the ovaries, namely esr1, esr2a, and esr2b, and two gonadotropin receptors, fshr and lhr. These results suggest that transient early-life exposure to environmentally relevant concentrations of DEHP can inhibit the reproduction of adult female zebrafish.</p>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":" ","pages":"110090"},"PeriodicalIF":3.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MEHP induced mitochondrial damage by promoting ROS production in CIK cells, leading to apoptosis, autophagy, cell cycle arrest","authors":"Lu Li ,&nbsp;Wan Li ,&nbsp;Yufeng Liu ,&nbsp;Bing Han ,&nbsp;Yanbo Yu ,&nbsp;Hongjin Lin","doi":"10.1016/j.cbpc.2024.110064","DOIUrl":"10.1016/j.cbpc.2024.110064","url":null,"abstract":"<div><div>Although Mono (2-ethylhexyl) phthalate (MEHP) is a metabolite of Di (2-ethylhexyl) phthalate (DEHP), it has been confirmed to exhibit stronger biological toxicity than DEHP. Mitochondrial dynamic homeostasis and normal mitochondrial function regulate numerous physiological and pathological processes. However, it remains unclear whether MEHP triggers apoptosis, autophagy, and cell cycle arrest in grass carp kidney (CIK) cells by causing mitochondrial damage. Here, we established a MEHP dose-dependent exposure models in CIK cells and treated them with NAC. The results demonstrated that MEHP promoted ROS production and decreased antioxidant enzyme activities in CIK cells in a concentration-dependent manner. MEHP destroyed mitochondrial homeostasis and mitochondrial function in CIK cells, manifested by decreasing mitochondrial membrane potential (MMP), down-regulating gene expression of fusion division genes including MFN1, MFN2, CLPP, DRP1, OPA1, and MFF, and reducing OXPHOS complex enzyme protein level including COXI, COXII, COXIII, COXIV, and COXV. In addition, MEHP treatment not only can increase the level of Cyt-c, Atg12, Atg13, Atg14, Beclin1, ULK1, LC3-II, Caspase3, Caspase9, and Bax, but also can decrease the level of Bcl2, p62, CyclinB, CyclinD, and CyclinE in a concentration-dependent manner, which resulted in apoptosis, autophagy and cell cycle arrest. Furthermore, MEHP dose-dependently nduced downregulation gene expression of immunoglobulins and antimicrobial peptides (Hepcidin, β-defensin, LEAP2). However, NAC treatment could significantly reverse the above changes and alleviate CIK cells damage caused by exposure to MEHP. This study has expanded our understanding about molecular mechanisms of MEHP toxicity in aquatic animals and provided a reference for comparative medicine research.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110064"},"PeriodicalIF":3.9,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal remodelling of the fish heart alters sensitivity to petrochemical pollutant, 3-methylphenanthrene","authors":"Irina Dzhumaniiazova ,&nbsp;Tatiana S. Filatova ,&nbsp;Artem Shamshura ,&nbsp;Denis V. Abramochkin ,&nbsp;Holly A. Shiels","doi":"10.1016/j.cbpc.2024.110082","DOIUrl":"10.1016/j.cbpc.2024.110082","url":null,"abstract":"<div><div>Exploitation of offshore oil reserves, heightened traffic in marine transportation routes, and the release of petrochemicals from the thawing of permafrost and glaciers is increasing the bioavailability of polycyclic aromatic hydrocarbons (PAHs) to aquatic organisms. This availability may also change with the seasons as temperature changes accessibility of Arctic transport routes and the degree of land- and ice-melt and thus run-off into coastal ecosystems. Seasonal temperature change also remodels the ion channels in the heart of fish to facilitated preserved cardiac function across a range of temperatures. How this seasonal cardiac remodelling impacts vulnerability to pollutants is currently unknown. In this study we accessed the electrical activity of navaga cod (<em>Eleginus nawaga</em>) ventricular cardiomyocytes under the dual influence of seasonal change and varying concentrations of a pervasive PAH pollutant, 3-methylphenanthrene (3-MP). We used whole-cell patch-clamp to elucidate the effect of various doses of 3-MP on action potential (AP) parameters and the main ion currents (I_Kr, I_K1, I_Na, I_Ca) in ventricular cardiomyocytes isolated from navaga cod in winter and summer at the White Sea, close to the Russian Arctic circle. Navaga cod ventricular cardiomyocytes were particularly vulnerable to 3-MP during the winter season. Exposure to 300 nM 3-MP resulted in significant changes in AP duration in winter-acclimatized fish, whereas no such changes were observed in summer-acclimatized fish. The I_Kr current was the most sensitive to 3-MP, with a winter IC₅₀ of 49.7 nM and a summer IC₅₀ of 53 μM. The I_Na current also exhibited seasonal shifts in sensitivity to 3-MP, with IC₅₀ values of 2.39 μM in winter-acclimatized fish and 7.73 μM in summer-acclimatized fish. No significant differences were observed in the effect of 3-MP on the peak I_Ca current, although 3 μM of 3-MP caused a pronounced decrease in charge transferred by I_Ca (e.g. Q_Ca) in both seasons. The I_K1 current was insensitive to 3-MP in both winter and summer fish. These findings highlight how remodelling of the fish heart with changing season alters the potency of PAH pollution. This paper lays the groundwork for future research on the molecular mechanisms that drive the altered seasonal potency of pollutants in navaga cod and other species.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110082"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of microplastics exposure on liver health: A comprehensive meta-analysis","authors":"Yizi Zhang ,&nbsp;Jiahui Yuan ,&nbsp;Ting Mao","doi":"10.1016/j.cbpc.2024.110080","DOIUrl":"10.1016/j.cbpc.2024.110080","url":null,"abstract":"<div><div>Microplastics (MPs) are significant concerns affecting liver health. This is the first comprehensive meta-analysis, evaluating the impact of MPs on liver functions across various animal models, including mice, fish, crabs, and shrimp. Five databases, including PubMed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), Ovid MEDLINE, and Web of Science, were used to select eligible studies. In all, 70 studies out of 1872 publications were included in the analysis, the impact of MPs on liver enzymes, oxidative stress markers, and inflammatory cytokines were evaluated. Our results revealed significant increases in liver enzymes ALT and AST, oxidative stress markers MDA, and pro-inflammatory cytokines IL-6 and TNF-<em>α</em>, along with a notable reduction in antioxidative enzymes like SOD, CAT, GSH, and GPx. These findings suggest that MPs exposure significantly disrupts liver function by inducing oxidative stress and inflammation. The results underscore the urgent need for targeted environmental policies and further research.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110080"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A time-dependent interactive effect of nitrite and ammonia on inflammatory and immune response in the head kidney of silver carp (Hypophthalmichthys molitrix)","authors":"Yiwen Li ,&nbsp;Honghui Guo ,&nbsp;Hen Ge ,&nbsp;Hang Sha ,&nbsp;Yidi Wu ,&nbsp;Guiwei Zou ,&nbsp;Hongwei Liang","doi":"10.1016/j.cbpc.2024.110078","DOIUrl":"10.1016/j.cbpc.2024.110078","url":null,"abstract":"<div><div>Nitrite and ammonia stress frequently have harmful effects on aquatic animals. However, the effect of ammonia combined with nitrite on immunity is unclear. Aimed to determine their interactive effect on head kidney, silver carp were exposed to ammonia (15 mg/L), nitrite (10 mg/L), or ammonia+nitrite (15 mg/L + 10 mg/L), and control conditions for 48 h. The results showed that exposure to nitrite and ammonia caused loss of cytoplasm and vacuolar degeneration of cells in head kidney. Following exposure to nitrite and ammonia, tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β) levels decreased significantly, while immunoglobulin M (IgM) and complement 3 (C3) levels increased significantly (<em>P</em> &lt; 0.05). Additionally, TNF-α and C3 were significantly affected synergistically by 24 h of exposure to ammonia+nitrite, while significantly antagonistic effect on IL-1β and C3 was observed after 48 h of exposure. After 48 h of exposure, differently expressed genes (DEGs) induced by ammonia, nitrite, and their combination were mainly assigned to molecular function and biological process, and complement and coagulation cascade pathway was enriched with the highest number of immunity-associated DEGs. Integrated biomarker response (IBR) in nitrite group was higher than that in ammonia group, and the combination of ammonia and nitrite increased and decreased the IBR induced by ammonia and nitrite after 24 and 48 h of exposure, respectively. In conclusion, the toxicities of ammonia and nitrite toward head kidney displayed inflammatory suppression and immune activation, and their had synergistic and antagonistic effects on head kidney at 24 h and 48 h, respectively.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110078"},"PeriodicalIF":3.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of an organophosphate pesticide and water restriction on physiology and immunity in the passerine Zonotrichia capensis","authors":"Benjamín Corvalan ,&nbsp;Felipe Alvarez-Vergara ,&nbsp;Diego Landaeta ,&nbsp;Natalia Ramirez-Otarola ,&nbsp;Juan Carlos Sanchez-Hernandez ,&nbsp;Karin Maldonado ,&nbsp;Roberto F. Nespolo ,&nbsp;Seth D. Newsome ,&nbsp;Pablo Sabat","doi":"10.1016/j.cbpc.2024.110076","DOIUrl":"10.1016/j.cbpc.2024.110076","url":null,"abstract":"<div><div>Climate change, specifically rising temperatures and increased frequency of droughts will increase the level of exposure of organisms to chemical pollution. Notably, the impact of increased frequency and duration of drought events and subsequent dehydration on pesticide toxicity remains largely unknown. We evaluated the combined effects of exposure to the pesticide Chlorpyrifos (CPF) and water restriction (WR) on the enzymatic activities of three plasma esterases (acetylcholinesterase, butyrylcholinesterase and carboxylesterase), basal metabolic rate (BMR), leukocyte profile (proportion of heterophils to lymphocytes; H:L ratio), and bactericidal capacity of plasma in the rufous-collared sparrow (<em>Zonotrichia capensis</em>), a common passerine bird in Chile. The activity of the enzyme carboxylesterase decreased in birds exposed only to CPF and birds exposed to both the CPF and WR. In the group exposed to both stressors, the butyrylcholinesterase activity was also reduced. The BMR exhibited an increase in both groups exposed to CPF, with a higher rise observed in the group exposed to both CPF and WR, while the group subjected to WR showed no change. The bactericidal capacity of the plasma decreased significantly in birds exposed to CPF and in those exposed to both the pesticide and WR, while a marginal effect was observed in the group only subjected to WR. The H:L ratio increased in all groups, with the most pronounced effect in birds exposed to both stressors. These results suggest a shift in the energy budget of <em>Z. capensis</em>, favoring the detoxification of the pesticide through esterase activities, at the expense of reduced immunocompetence.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110076"},"PeriodicalIF":3.9,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis of gill tissue response to hypoxia stress in the Chinese mitten crab (Eriocheir sinensis)","authors":"Xue Chen ,&nbsp;Yulin Bai ,&nbsp;Junlei Ma ,&nbsp;Anqi Wang ,&nbsp;Dongpo Xu","doi":"10.1016/j.cbpc.2024.110081","DOIUrl":"10.1016/j.cbpc.2024.110081","url":null,"abstract":"<div><div>Crustaceans often encounter the occurrence of various hypoxic situations, and in order to cope with this situation, they have evolved a series of antioxidant defenses against hypoxic stress. The present study was conducted to investigate the physiological and molecular regulation of hypoxic stress in the Chinese mitten crab (<em>Eriocheir sinensis</em>). We used the method of reducing dissolved oxygen in water to treat the juvenile <em>E. sinensis</em> with hypoxia. The results showed that total antioxidant capacity, superoxide dismutase, catalase and malondialdehyde contents in the gills of juvenile crabs were significantly elevated under hypoxia. In addition, gill tissues from normoxic control (NC), hypoxia-sensitive (HS) and hypoxia-tolerant (HT) groups were analysed using transcriptomic sequencing. The results revealed that 2124, 2946 and 2309 differentially expressed genes (DEGs) were found in NC vs. HS, NC vs. HT and HS vs. HT, respectively. The analysis of KEGG pathway enrichment indicated DEGs were predominantly enriched in oxidative phosphorylation, adipocytokine signaling pathway, and protein processing in endoplasmic reticulum in HS vs. HT. Enrichment of the MAPK signaling pathway, apoptosis, glucagon signaling pathway, and arachidonic acid metabolism was also found in the comparisons of NC vs. HS and NC vs. HT. The DEGs in these pathways may play a key role in gill tolerance to hypoxia. These results provide new insights and references for the oxidative defense and adaptive regulatory mechanisms of gill tissues of juvenile <em>E. sinensis</em> in response to hypoxic stress.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110081"},"PeriodicalIF":3.9,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of a novel furan hybrid chalcone against bisphenol A-induced craniofacial developmental toxicity in zebrafish embryos","authors":"B. Haridevamuthu ,&nbsp;S. Madesh ,&nbsp;Ankit Kumar Bharti ,&nbsp;L.S. Dhivya ,&nbsp;Rajakrishnan Rajagopal ,&nbsp;Ahmed Alfarhan ,&nbsp;Kathiravan Muthu Kumaradoss ,&nbsp;Jesu Arockiaraj","doi":"10.1016/j.cbpc.2024.110072","DOIUrl":"10.1016/j.cbpc.2024.110072","url":null,"abstract":"<div><div>Bisphenol A (BPA), a pervasive endocrine disruptor, is known to cause significant developmental toxicity, particularly affecting craniofacial structures through oxidative stress and apoptosis. A novel furan hybrid chalcone derivative, 3-(2-hydroxy-5-nitrophenyl)-1-(5-methylfuran-2-yl)prop-2-en-1-one (DK04), specifically with a hydroxyl group for its antioxidant properties and a nitro group for enhanced electron-withdrawing ability, was evaluated for its potential to mitigate these toxic effects. Zebrafish embryos were exposed to BPA and co-treated with various concentrations of DK04. Our results demonstrated that DK04 significantly reduced reactive oxygen species (ROS) generation and lipid peroxidation, increased antioxidant enzyme activities (SOD and CAT), and restored the balance between pro-apoptotic (<em>p53</em>) and anti-apoptotic (<em>bcl2</em>) genes. Furthermore, DK04 treatment improved bone mineralization and chondrogenesis by reversing BPA-induced disruptions in osteogenic markers (<em>runx2</em>, <em>sox9a</em>, <em>bmp6</em>, and <em>mmp13a</em>). The locomotion impairments observed in BPA-exposed embryos were also ameliorated by DK04, indicating its potential neuroprotective effects. These findings suggest that DK04 offers a multifaceted approach to counteract BPA toxicity, making it a promising candidate for therapeutic intervention. This research underscores the importance of developing prophylactic compounds to safeguard health against environmental toxicants like BPA. Future studies should focus on long-term safety and efficacy in mammalian models and explore synergistic effects with other protective agents to broaden the applications of DK04 and contribute to public health benefits.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110072"},"PeriodicalIF":3.9,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}