Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"Warming-induced microplastic accumulation and physiological toxicity in fiddler crabs","authors":"Karen Beltran-de la Torre ,&nbsp;Jorge Arturo Vargas-Abúndez ,&nbsp;Ricardo Dzul-Caamal ,&nbsp;Anieli C. Maraschi ,&nbsp;Mariana V. Capparelli","doi":"10.1016/j.cbpc.2025.110142","DOIUrl":"10.1016/j.cbpc.2025.110142","url":null,"abstract":"<div><div>Under natural conditions, organisms are exposed to multiple stressors simultaneously, such as microplastic (MP) contamination and rising global temperatures. To assess the combined effects of acute MP exposure and increasing temperatures on the fiddler crab <em>Minuca rapax</em>, we exposed the crabs to polyethylene microspheres (0 and 2 mg L⁻¹, size 53–63 μm) at three different temperatures (24, 27, and 30 °C). Physiological responses were assessed by measuring oxygen consumption and evaluating the biochemical activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) alongside lipid peroxidation (LPO) in the gills and hepatopancreas. MP bioaccumulation was quantified in the gills, digestive tract, and muscles. Our findings revealed that MP bioaccumulation was highest in the gills, followed by the digestive tract and muscles. Notably, elevated temperatures (30 °C) suppressed MP accumulation. At 30 °C, MP-exposed crabs showed increased oxygen consumption, while at 27 °C, SOD and GPx activities were elevated. In contrast, in MP-exposed crabs, catalase activity and LPO levels decreased at 30 °C. Overall, the combined effects of MP exposure and temperature-induced stress exacerbated physiological toxicity in <em>Minuca rapax</em>, underscoring the importance of considering multiple environmental stressors when evaluating the impacts of MP contamination.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"290 ","pages":"Article 110142"},"PeriodicalIF":3.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genus-wide study on venom proteome variation and phospholipase A2 inhibition in Asian lance-headed pit vipers (genus: Trimeresurus)","authors":"Mun Yee Yong ,&nbsp;Kae Yi Tan ,&nbsp;Choo Hock Tan","doi":"10.1016/j.cbpc.2024.110077","DOIUrl":"10.1016/j.cbpc.2024.110077","url":null,"abstract":"<div><div>High molecular weight proteins are present abundantly in viperid venoms. The amino acid sequence can be highly variable, contributing to the structure and function diversity of snake venom protein. However, this variability remains poorly understood in many species. The study investigated the venom protein variability in a distinct clade of Asian pit vipers (<em>Trimeresurus</em> species complex) through comparative proteomics, applying gel electrophoresis (SDS-PAGE), liquid chromatography-tandem mass spectrometry (LCMS/MS), and bioinformatic approaches. The proteomes revealed a number of conserved protein families, within each are variably expressed protein paralogs that are unrelated to the snake phylogeny and geographic origin. The expression levels of two major enzymes, i.e., snake venom serine proteinase and metalloproteinase, correlate weakly with procoagulant and hemorrhagic activities, implying co-expression of other functionally versatile toxins in the venom. The phospholipase A₂ (PLA₂) abundance correlates strongly with its enzymatic activity, and a unique phenotype was discovered in two species expressing extremely little PLA₂. The commercial mono-specific antivenom effectively neutralized the venoms' procoagulant and hemorrhagic effects but failed to inhibit the PLA₂ activities. Instead, the PLA₂ activities of all venoms were effectively inhibited by the small molecule inhibitor varespladib, suggesting its potential to be repurposed as a highly potent adjuvant therapeutic in snakebite envenoming.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"288 ","pages":"Article 110077"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive toxicity and transgenerational effects of co-exposure to polystyrene microplastics and arsenic in zebrafish","authors":"Yuxuan Luo ,&nbsp;Zheng Zhang ,&nbsp;Xuewei Li ,&nbsp;Zile Zhuang ,&nbsp;Yihan Li ,&nbsp;Xinya Wang ,&nbsp;Changqing Liao ,&nbsp;Lujia Chen ,&nbsp;Qizhi Luo ,&nbsp;Xuncai Chen","doi":"10.1016/j.cbpc.2025.110134","DOIUrl":"10.1016/j.cbpc.2025.110134","url":null,"abstract":"<div><div>Microplastics (MPs) are ubiquitous environmental pollutants that have garnered significant attention due to their small particle size, resistance to degradation and large specific surface area, which makes it easy to adsorb various pollutants, particularly heavy metals. Arsenic (As), a common metal poisons, poses significant risks due to its widespread industrial use. When MPs and As co-exist in the environment, they can exert combined toxic effects on organisms, affecting various systems, including the nervous system. However, research on the reproductive damage caused by the co-exposure to MPs and As is limited, and the toxic effects and mechanisms remain unclear. In this study, we investigated the <em>co</em>-exposure of polystyrene microplastics (PSMP) and As on female zebrafish to evaluate the reproductive toxicity and transgenerational effects. The results revealed that the combined exposure exhibited elevated reproductive toxicity, resulting in reduced gonadal indices, abnormal oocyte maturation, and disrupted sex hormone levels, as evidenced by an increased E2/T ratio. Metabolomics analyses revealed that the co-exposure to PSMP and As primarily affected pathways involved in aminoacyl-tRNA biosynthesis, sphingolipid metabolism, linoleic acid metabolism, galactose metabolism, and amino sugar and nucleotide sugar metabolism. These pathways are associated with oxidative stress, lipid synthesis, and sex hormone synthesis. Importantly, the combined exposure group exhibited more pronounced effects on offspring development compared to the alone treatment group, characterized by increased mortality rate, decreased hatching rate, and reduced heart rate. These findings provide evidence that co-exposure to MPs and As damages the reproductive system and adversely affects offspring growth and development.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"290 ","pages":"Article 110134"},"PeriodicalIF":3.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of in vitro cytochalasin D and hypoxia on mitochondrial energetics and biogenesis, cell signal status and actin/tubulin/Hsp/MMP entity in air-breathing fish heart","authors":"S. Rekha ,&nbsp;M.C. Subhash Peter","doi":"10.1016/j.cbpc.2025.110132","DOIUrl":"10.1016/j.cbpc.2025.110132","url":null,"abstract":"<div><div>The cardiac actin cytoskeleton has a dynamic pattern of polymerisation. It is uncertain how far actin destabilisation impacts mitochondrial energetics and biogenesis, cell signal status, and structural entities in cardiomyocytes, particularly in hypoxic conditions. We thus tested the <em>in vitro</em> action of cytochalasin D (Cyt D), an inhibitor of actin polymerisation, in hypoxic ventricular explants to elucidate the role of the actin in mitochondrial energetics and biogenesis, cell signals and actin/tubulin/hsps/MMPs dynamics in hypoxic air-breathing fish hearts. The COX activity increased upon Cyt D exposure, whereas hypoxia lowered COX and SDH activities but increased LDH activity. The ROS increased, and NO decreased by Cyt D. COX and LDH activities, and NO content reversed after Cyt D exposure in hypoxic hearts. Cyt D exposure upregulated actin isoform expression (<em>Actc1</em> and <em>Actb1</em>) but downregulated tubulin isoform (<em>Tedc1</em>). Hypoxia upregulated actin (<em>Acta1a</em>, <em>Actb1</em>, <em>Actb2</em>, <em>Actc1a</em>) tubulin (<em>Tuba</em>, <em>Tubb5</em>, <em>Tedc1</em>, <em>Tubd1</em>) and hsp (<em>Hspa5</em>, <em>Hspa9</em>, <em>Hspa12a</em>, <em>Hspa14</em>, <em>Hspd1</em>, <em>Hsp90</em>) isoform transcript expression and Cyt D in hypoxic hearts reversed these isoform's expression. Hypoxia upregulated <em>Mmp2</em> and 9 transcript expressions but downregulated <em>Mfn1</em>, <em>Fis1</em>, <em>Nfkb1</em>, <em>Prkacaa</em>, and <em>Aktip</em> expressions, and Cyt D exposure reversed almost all these markers in hypoxic hearts. The data provide novel evidence for the mechanistic role of actin in integrating mitochondrial energetics and biogenesis, cell signal status and actin/tubulin/Hsp/MMP entity, indicating its critical cardioprotective role in defending against hypoxia. Besides proposing an air-breathing fish heart as a model, the study further brings the therapeutic potential of Cyt D towards hypoxia intervention.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"290 ","pages":"Article 110132"},"PeriodicalIF":3.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characteristics of aminotransferases gene family in Ruditapes philippinarum and its response to salinity stresses","authors":"Tao Liu,&nbsp;Hongtao Nie,&nbsp;Zhongming Huo,&nbsp;Xiwu Yan","doi":"10.1016/j.cbpc.2025.110133","DOIUrl":"10.1016/j.cbpc.2025.110133","url":null,"abstract":"<div><div>Aminotransferase is involved in the regulation of amino acid metabolism, which can affect the balance and distribution of amino acids in the organism, help maintain the homeostasis of amino acids in the organism, and play an important role in the environmental adaptation of aquatic animals. In this study, a total of 28 aminotransferase genes were identified in the genome of <em>R. philippinarum</em>. The gene structure, protein structure, chromosome localization, and phylogenetic analysis of aminotransferase were conducted using bioinformatics. According to the gene structure and phylogenetic analysis of aminotransferase proteins, aminotransferase proteins can be categorized into class I and II, class III, and class V. RNA-seq data analysis showed that aminotransferase genes were differentially expressed at different developmental stages, tissues, and salinity stress. In addition, qPCR demonstrated that the expression levels of most aminotransferase genes increased significantly during salinity changes. We also measured the free amino acids content in the gills of <em>R. philippinarum</em> after 48 h of low and high salinity stress. The results indicated that the total free amino acids under low salinity stress (75.89 ± 3.31 mg/g) and high salinity stress (91.01 ± 3.31 mg/g) at 48 h were significantly decreased and increased compared with the control group (83.01 ± 3.12 mg/g), respectively. The results of this study provide a valuable reference for further research on the salinity adaptation of the aminotransferase gene in <em>R. philippinarum</em>.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"290 ","pages":"Article 110133"},"PeriodicalIF":3.9,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual dimorphism in zebrafish aggression and metabolism under acute ammonia stress","authors":"Fang Zhi Chua ,&nbsp;Li-Yih Lin ,&nbsp;Yung-Che Tseng ,&nbsp;Ming-Yi Chou","doi":"10.1016/j.cbpc.2025.110131","DOIUrl":"10.1016/j.cbpc.2025.110131","url":null,"abstract":"<div><div>Animals must adapt their behaviors in response to environmental stressors to enhance survival prospects. Aquatic organisms, particularly teleost fish, face unique environmental challenges, making them ideal models for studying environmental stress adaptation. While previous research on acute environmental stress acclimation provided valuable insights, it often overlooked potential sex-specific responses. Growing evidence suggests significant sexual dimorphism in physiological and behavioral responses to various environmental stressors. This emerging paradigm reveals a critical knowledge gap in our understanding of sex-specific stress acclimation strategies and their underlying mechanisms in teleost fish. To address this gap, we investigated the effects of acute ammonia exposure, a common aquatic stressor, on male and female zebrafish. We examined differential behaviors and metabolic rates between the sexes under ammonia stress and found sex-specific responses: males tended to recover aggression and reduced fighting latency without affecting outcomes, whereas females exhibited lowered oxygen consumption and reduced aggression. These findings highlight differences in acute stress adaptation strategies between males and females, contributing to a more-comprehensive understanding of sex-specific stress adaptation in aquatic environments and underscoring the importance of considering sexual dimorphism in environmental stress studies.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"290 ","pages":"Article 110131"},"PeriodicalIF":3.9,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paraquat neurotoxicity: Oxidative stress and neuronal dysfunction in the ascidian brain","authors":"Rebeca Reis e Silva ,&nbsp;Taynan Motta Portal ,&nbsp;Nathany da Silva Nogueira ,&nbsp;Thuany da Silva Nogueira ,&nbsp;Andressa de Abreu Mello ,&nbsp;Cintia Monteiro-de-Barros","doi":"10.1016/j.cbpc.2025.110128","DOIUrl":"10.1016/j.cbpc.2025.110128","url":null,"abstract":"<div><div>Paraquat (PQ) is a widely used herbicide; however, it has been linked to various diseases, including an increased risk of developing Parkinsonism. To study this, invertebrates such as ascidians have been used. They have a simple nervous system and are considered an emerging model for the study of neurodegenerative diseases. Here, we investigated the effects of PQ in the brain of the ascidian <em>Styela plicata</em>. We performed behavioral tests, assessed morphology, and monitored oxidative stress and the expression of tyrosine hydroxylase (TH) and caspase-3 (Casp 3) using immunofluorescence. In the presence of PQ, siphon movement was reduced and cortical thickness was increased. In addition, an observed increase in ROS was detected, along with attenuated SOD and CAT activities and increased LPO production. Interestingly, PQ downregulated the expression of TH and upregulated that of Casp 3. We conclude that PQ-induced oxidative stress leads to the death of catecholaminergic neurons in the <em>S. plicata</em> brain.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"290 ","pages":"Article 110128"},"PeriodicalIF":3.9,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative biology of chlorantraniliprole selected and unselected Chrysoperla carnea (Stephens) populations: Stability of resistance, inheritance mode, and realized heritability","authors":"Muhammad Abubakar ,&nbsp;Sarfraz Ali Shad","doi":"10.1016/j.cbpc.2025.110125","DOIUrl":"10.1016/j.cbpc.2025.110125","url":null,"abstract":"<div><div>Insecticide resistance in natural enemies can be used as a positive trait in integrated pest management programs by increasing the compatibility of two important tools; biological and chemical control. In this experiment, a field population of <em>Chrysoperla carnea</em> (Stephens) (Neuroptera: Chrysopidae) was selected with chlorantraniliprole for 35 generations (Chlor-Sel) developed a 100.32-fold resistance level compared to an unselected population (Unsel). The dominance values of resistance for reciprocal crosses confirmed incomplete dominance inheritance of chlorantraniliprole resistance. The results on projected rate of chlorantraniliprole resistance showed that at 10 % to 90 % survival in each selection and a realized heritability value of 0.19, the Chlor-Sel will require only 3 to 26 generations (Slope = 1.15) to increase a 10-fold level of resistance. The results of the age-stage life table experiment showed that fecundity, oviposition days, male and female longevity, net reproductive rate (Ro), and gross reproductive rate (GRR) of both crosses were significantly increased compared to their parents. The fecundity of reproductive females, male and female longevity, Ro, generation time (<em>T</em>), and GRR of Chlor-Sel were not significantly different than that of the Unsel strain of <em>C. carnea</em>. In conclusion, chlorantraniliprole resistance in <em>C. carnea</em> has an autosomal, polygenic, and incompletely dominant inheritance mode and <em>C. carnea</em> has potential to develop resistance that leads to fitness benefits. This information will help in management of insect pests by deploying resistant strains with selected insecticides and conservation of these natural enemies in fields with sustaining resistance genes.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110125"},"PeriodicalIF":3.9,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deleted in malignant brain tumors 1 (DMBT1) gene relate to immune priming and phagocytosis modulation in the small abalone Haliotis diversicolor","authors":"Tuo Yao ,&nbsp;Junyuan Tan ,&nbsp;Jie Lu ,&nbsp;Shengli Fu ,&nbsp;Zhilv Xie ,&nbsp;Bing Li ,&nbsp;Changhong Cheng ,&nbsp;Lingtong Ye","doi":"10.1016/j.cbpc.2025.110126","DOIUrl":"10.1016/j.cbpc.2025.110126","url":null,"abstract":"<div><div>The small abalone (<em>Haliotis diversicolor</em>) is an economic shellfish cultured in the south coast of China. In recent years, the frequent occurrence of the disease has led to significant mortality in abalone farms. Deleted in malignant brain tumors 1 (DMBT1), a member of the scavenger receptor cysteine-rich (SRCR) protein family, plays an important role in host defense. However, its function in <em>H. diversicolor</em> remains unknown. In order to evaluate the immune priming effect after secondary infection and elucidate possible regulatory mechanism, a novel DMBT1 from the small abalone <em>H. diversicolor</em> (designated as HdDMBT1) was cloned and characterized in this study. The open reading frame of HdDMBT1 was 2331 bp encoding 776 amino acids with a molecular weight of 84.73 kDa. HdDMBT1 contained conserved active sites with DMBT1 from other species, detected in all tested tissues and had higher expression levels in hepatopancreas. The temporal expression profiles of HdDMBT1 after two challenges of <em>Vibrio harveyi</em> were examined to evaluate priming response in the small abalone. The expression level of HdDMBT1 mRNA in hepatopancreas increased significantly after <em>V. harveyi</em> challenge. Meanwhile, the expression level of HdDMBT1 after the second challenge was significantly higher than that after the first challenge (4.23-fold). RNA interference (RNAi) experiments were conducted to examine the role of HdDMBT1 in response to <em>V. harveyi</em> infection. Knocking down HdDMBT1 decreased the hemocytes phagocytosis (0.48-fold). In addition, the bacterial density in hemolymph and the mortality of abalone raised, when infected with <em>V. harveyi</em> after dsHdDMBT1 injection. These results indicated that HdDMBT1 might play an important role in tolerance to bacterial infection.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"290 ","pages":"Article 110126"},"PeriodicalIF":3.9,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA damage triggers the death of green sea turtle-derived cells at high temperature","authors":"Masafumi Katayama ,&nbsp;Satomi Kondo ,&nbsp;Manabu Onuma ,&nbsp;Shouta M.M. Nakayama ,&nbsp;Tomokazu Fukuda","doi":"10.1016/j.cbpc.2025.110127","DOIUrl":"10.1016/j.cbpc.2025.110127","url":null,"abstract":"<div><div>As temperatures rise due to increasingly severe global warming, the effect of high temperatures on wildlife, including green sea turtles, is one of the issues that must be addressed to ensure the conservation of biodiversity. In the current study, we found that green sea turtle cell death due to apoptosis occurred at 37 °C, which suppressed cell proliferation. We also found that high temperature-induced heat stress led to the accumulation of DNA damage in green sea turtle cells. DNA damage is a key factor for the induction of apoptosis. Therefore, heat stress-mediated DNA damage can trigger green sea turtle cell apoptosis. Based on these results, we predict that high temperatures, such as 37 °C, would adversely impact green sea turtles, resulting in death or health-related challenges. Importantly, this information can facilitate the conservation of green sea turtles because cellular and molecular studies regarding the effects of global warming effects remain limited.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110127"},"PeriodicalIF":3.9,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}