Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献_第3页

Gene-metabolite correlations underpin the hepatic fibrosis and metabolic disruption induced by methyltestosterone in juvenile Chinese longsnout catfish (Leiocassis longirostris) 基因-代谢物相关性支持甲基睾酮诱导的中国长鼻鲶鱼幼鱼肝纤维化和代谢紊乱

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-27 DOI: 10.1016/j.cbpc.2025.110260

Yang Feng , Yongqiang Deng , Zhao Li , Zheng Wei , Zhongmeng Zhao , Han Zhao , Senyue Liu , Lu Zhang , Yuanliang Duan , Zhipeng Huang , Jun Du , Qiang Li , Jian Zhou , Chengyan Mou

{"title":"Gene-metabolite correlations underpin the hepatic fibrosis and metabolic disruption induced by methyltestosterone in juvenile Chinese longsnout catfish (Leiocassis longirostris)","authors":"Yang Feng , Yongqiang Deng , Zhao Li , Zheng Wei , Zhongmeng Zhao , Han Zhao , Senyue Liu , Lu Zhang , Yuanliang Duan , Zhipeng Huang , Jun Du , Qiang Li , Jian Zhou , Chengyan Mou","doi":"10.1016/j.cbpc.2025.110260","DOIUrl":"10.1016/j.cbpc.2025.110260","url":null,"abstract":"<div><div>Methyltestosterone (MT), a synthetic androgen frequently employed in aquaculture to induce sex reversal and boost growth rates, has yet to be extensively examined for its enduring effects on the health of fish. This study systematically evaluated the toxic effects of MT on the liver of Chinese longsnout catfish (<em>Leiocassis longirostris</em>) using an integrated approach of histopathology, transcriptomics, and metabolomics. The fish were subjected to three doses of MT (100 ng, 200 ng, and 300 ng) over 30 days, followed by pathological examinations of liver tissue. The study unveiled a novel discovery that MT induced perivascular fibrosis and abnormal protein accumulation in the liver, which bore a striking resemblance to effects seen in humans. Transcriptomic analysis pinpointed differentially expressed genes (DEGs) that were predominantly enriched in metabolic pathways, including those related to PPAR signaling, nitrogen metabolism, and cholesterol metabolism. Metabolomic analysis further revealed significant disruptions in the metabolic profiles of MT-treated fish, particularly in fatty acid and amino acid metabolism pathways. Key differential metabolites, such as oleic acid, phosphocholine, and various lipids and amino acids, were identified through VIP value screening. Correlation analysis showed MT induced liver fibrosis via upregulating fibrosis-related COL6A6 and disease related genes (<em>F2R</em>, <em>ESR1</em>, <em>PGR</em>). MT also induced lipid anabolism upregulation and amino acid metabolism suppression, linking MT to NAFLD-like obesity in fish. This study offers comprehensive insights into MT's hepatic toxicity, guiding safe aquaculture practices.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110260"},"PeriodicalIF":3.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144516957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interaction between cardiovascular system and thyroid: Atrial natriuretic peptide in the thyroid function of the lizard Podarcis siculus: In vivo experiments and immunolocalisation 心血管系统和甲状腺之间的相互作用：心钠肽在蜥蜴足猿甲状腺功能中的作用：体内实验和免疫定位。

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-25 DOI: 10.1016/j.cbpc.2025.110259

Rosaria Sciarrillo , Alessandra Falzarano , Vito Gallicchio , Assunta Lallo , Francesca Carrella , Aldo Mileo , Anna Capaldo , Maria De Falco

{"title":"The interaction between cardiovascular system and thyroid: Atrial natriuretic peptide in the thyroid function of the lizard Podarcis siculus: In vivo experiments and immunolocalisation","authors":"Rosaria Sciarrillo , Alessandra Falzarano , Vito Gallicchio , Assunta Lallo , Francesca Carrella , Aldo Mileo , Anna Capaldo , Maria De Falco","doi":"10.1016/j.cbpc.2025.110259","DOIUrl":"10.1016/j.cbpc.2025.110259","url":null,"abstract":"<div><div>Although the relationship between thyroid and heart has been extensively studied, especially in cases of heart disease, the hypothesis of a relationship between heart and thyroid is still far from being fully understood. The possible involvement of atrial natriuretic peptide (ANP) in thyroid regulation is supported by the immunohistochemical identification of the peptide in thyroid follicular cells of the lizard <em>Podarcis siculus</em>. The aim of this work was to study the action of ANP on the metabolism of thyroid hormones and to analyze the mechanism of ANF action in lizard thyroid follicles. Intraperitoneally administration of ANP (1–4 μg/100 g body weight) to <em>Podarcis siculus</em> lizards both after 2 h and after 24 h inhibited circulating plasma levels of T<sub>3</sub> and T<sub>4</sub> resulting in stimulation of TSH (Thyroid-Stimulating Hormone) and decrease of TRH (Thyrotropin Releasing Hormone). The inhibitory effect of ANP on the thyroid cells could be mediated by cGMP, which is one of the main mediators of ANP action. A stimulation of the level of 5-T4 ORD (type II) Monodeiodinase activity at the hepatic level by ANP increased hepatic T<sub>3</sub> levels and decreased hepatic T<sub>4</sub> levels, revealing an alternative mode of signalling by ANP on peripheral biosynthesis of thyroid hormones. In conclusion, our results indicate the ANP role in the regulation of thyroid hormone synthesis and secretion, supported by the immunohistochemical presence of the peptide in the apical region of thyroid follicular cells and in the fibres surrounding lizard follicles, which could underlie an ANP-mediated autocrine and paracrine regulatory pathway.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110259"},"PeriodicalIF":3.9,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

7-chloro-4-(phenylselanyl)quinoline exerts protective effect on acrylamide-induced neurotoxicity in the fly Drosophila melanogaster through modulation of acetylcholinesterase and oxidative stress 7-氯-4-（苯selanyl）喹啉通过调节乙酰胆碱酯酶和氧化应激对丙烯酰胺诱导的黑腹果蝇神经毒性具有保护作用。

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-23 DOI: 10.1016/j.cbpc.2025.110257

Elize Musachio , Francielli Polet Almeida , Márcia Rósula Poetini , Dieniffer Espinosa Janner , Diego Alves , Gustavo Guerra , Marina Prigol

{"title":"7-chloro-4-(phenylselanyl)quinoline exerts protective effect on acrylamide-induced neurotoxicity in the fly Drosophila melanogaster through modulation of acetylcholinesterase and oxidative stress","authors":"Elize Musachio , Francielli Polet Almeida , Márcia Rósula Poetini , Dieniffer Espinosa Janner , Diego Alves , Gustavo Guerra , Marina Prigol","doi":"10.1016/j.cbpc.2025.110257","DOIUrl":"10.1016/j.cbpc.2025.110257","url":null,"abstract":"<div><div>This study aimed to evaluate the neuroprotective action of 7-chloro-4-(phenylselanyl)quinoline (4-PSQ) through the activity of the enzyme acetylcholinesterase (AChE), oxidative and behavioral parameters associated with acrylamide-induced neurotoxicity (ACR) in <em>Drosophila melanogaster</em> flies. The flies were divided into Control, 4-PSQ (25 μM), ACR (5 mM) and ACR + 4-PSQ (concomitantly) groups, and remained in treatments for 4 days. All analyses were performed with head samples. It was observed that flies belonging to the ACR + 4-PSQ group presented levels of reactive species and lipid peroxidation similar to the control, and similarly aligned the activity of the enzyme's superoxide dismutase, catalase and glutathione S-transferase, culminating in the preservation of mitochondrial and cellular metabolic capacity. There was a positive modulation of the activity of the AChE enzyme, which was attributed to the conservation of locomotor and exploratory behaviors, configuring the neuroprotective role of 4-PSQ. Together, these results may have reflected in the survival of the flies, which obtained a rate similar to the control. Considering the presence of ACR in the population's life, it was applied to the induction of a neurotoxicity model in <em>Drosophila melanogaster</em>, and thus, these results are scientifically relevant, as they conclude that 4-PSQ presented antioxidant and neuroprotective action.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110257"},"PeriodicalIF":3.9,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The tissue-specific ferroptosis in zebrafish (Danio rerio) exposed to dibutyl phthalate at environmental-relevant concentrations 斑马鱼（Danio rerio）暴露于环境相关浓度的邻苯二甲酸二丁酯的组织特异性铁下垂

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-23 DOI: 10.1016/j.cbpc.2025.110258

Tingting Hou , Fei Wang , Lei Wang , Huiqiang Zhang , Xiaoteng Fan

{"title":"The tissue-specific ferroptosis in zebrafish (Danio rerio) exposed to dibutyl phthalate at environmental-relevant concentrations","authors":"Tingting Hou , Fei Wang , Lei Wang , Huiqiang Zhang , Xiaoteng Fan","doi":"10.1016/j.cbpc.2025.110258","DOIUrl":"10.1016/j.cbpc.2025.110258","url":null,"abstract":"<div><div>Dibutyl phthalate (DBP) is extensively contaminated in aquatic environment with severe threats to fishes, its toxic mechanisms and the susceptibility across different tissues require further investigations. In this study, zebrafish (<em>Danio rerio</em>) were used to investigate the histopathology with ferroptosis responses to DBP exposure through physiological and biochemical detection, histological examination, and transcript analyses. Following the chronic DBP exposure at environmental-relevant concentrations (0, 5, and 50 μg/L), the body weight, body length, condition factor, and gonadosomatic index of female zebrafish were decreased, along with a slightly increased hepatosomatic index and retarded development. Notably, the total iron content and distribution were elevated in the liver and brain, accompanied by increased lipid peroxidation and decreased glutathione peroxidase 4 (GPX4) and glutathione (GSH) levels, ultimately leading to histological impairments. Transcript analyses showed that DBP induced ferroptosis by down-regulating genes expression of system Xc<sup>−</sup> and <em>gpx4b</em>, while up-regulating genes expression related to iron metabolism and lipid peroxidation in the liver. In the brain, similarly ferroptosis responses were observed, characterized by the downregulation of <em>gpx4b</em> and upregulation of iron metabolism-related genes and <em>acsl4a</em> expression. In contrast, the gill and ovary exhibited oxidative stress, lipid peroxidation, structural disruption or follicular retardation without pronounced ferroptosis response. Taken together, this study demonstrates that DBP exposure could induce ferroptosis in zebrafish in a tissue-specific manner, that contributes to an in-depth understanding of the biotoxicity and tolerance to chemical contamination.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110258"},"PeriodicalIF":3.9,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and characterization of the carbonic anhydrase gene family reveals carbonate alkalinity adaptability in Chinese mitten crab (Eriocheir sinensis) 中华绒螯蟹碳酸酐酶基因家族的全基因组鉴定及其对碳酸盐碱度的适应性

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-17 DOI: 10.1016/j.cbpc.2025.110255

Yulin Bai , Junlei Ma , Zihan Fan , Anqi Wang , Dongpo Xu

{"title":"Genome-wide identification and characterization of the carbonic anhydrase gene family reveals carbonate alkalinity adaptability in Chinese mitten crab (Eriocheir sinensis)","authors":"Yulin Bai , Junlei Ma , Zihan Fan , Anqi Wang , Dongpo Xu","doi":"10.1016/j.cbpc.2025.110255","DOIUrl":"10.1016/j.cbpc.2025.110255","url":null,"abstract":"<div><div>Carbonic anhydrase (CA) is an essential zinc metalloenzyme that plays a crucial role in the physiological process of acid-base regulation in aquatic species. The <em>CA</em> gene family has been characterized in multiple species, but a comprehensive analysis of the <em>CA</em> gene family in <em>Eriocheir sinensis</em> is yet to be conducted. In this study, we identified members of the <em>CA</em> gene family from the <em>E. sinensis</em> genome and analyzed their genomic physical distribution, structural characteristics, expression patterns, and cellular localization. The results revealed a total of 15 <em>CA</em> genes in <em>E. sinensis</em>, distributed across 12 chromosomes, with tandem duplication observed on chr43. Phylogenetic analysis indicated that the 39 <em>CA</em> genes from <em>E. sinensis</em>, <em>Procambarus clarkii</em>, and <em>Portunus trituberculatus</em> can be classified into 4 clades and 11 subclades, with events of gene duplication and loss observed. Transcriptome expression analysis suggested that the <em>EsiCA7</em> gene might play a dominant role in the acid-base regulation of <em>E. sinensis</em>. Furthermore, subcellular localization experiments for the <em>EsiCA1</em> and <em>EsiCA7</em> genes confirmed the predictions made by bioinformatics analysis. In conclusion, this study provides a theoretical basis and molecular targets for further research on the function of the <em>CA</em> gene family and the mechanism of alkalinity tolerance in <em>E. sinensis</em>.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110255"},"PeriodicalIF":3.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quinoxyfen 1995-2019 is the story's end? Evaluation of its adverse effects on head size and nervous system genes involved in synaptic maturation 喹诺芬1995-2019是故事的结局吗？评估其对头大小和参与突触成熟的神经系统基因的不利影响。

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-16 DOI: 10.1016/j.cbpc.2025.110252

Annamaria Iannetta , Silvana Zugaro , Giovanni Angelozzi , Francesca Mazza , Marco Minacori , Tommaso Silvestrini , Elisabetta Benedetti , Monia Perugini

{"title":"Quinoxyfen 1995-2019 is the story's end? Evaluation of its adverse effects on head size and nervous system genes involved in synaptic maturation","authors":"Annamaria Iannetta , Silvana Zugaro , Giovanni Angelozzi , Francesca Mazza , Marco Minacori , Tommaso Silvestrini , Elisabetta Benedetti , Monia Perugini","doi":"10.1016/j.cbpc.2025.110252","DOIUrl":"10.1016/j.cbpc.2025.110252","url":null,"abstract":"<div><div>In 2013 quinoxyfen (QXY) was included in the list of priority hazard pollutants of the European Water Framework Directive due to its toxicity to aquatic organisms. The use of products formulated containing QXY was banned from all commercials starting from 27th June of 2019. QXY is an organic pollutant with potential persistence, bioaccumulation and this must not lower attention to this pollutant. To date the effects of this compound on development are not completely clear, thus this research tries to elucidate the adverse effects of QXY on zebrafish development. The study aims to understand the toxicological effects of QXY using the zebrafish as in vivo model and performing toxicological and molecular investigations. Considering the FET test results two sublethal concentrations, 0.4 mg/L and 0.8 mg/L were chosen for subsequent analysis. The expression of the <em>gad1b, cyp19a1b, shank3a, nrxn1a</em> and <em>c-fos</em> genes, involved in the development of the nervous system and the regulation of synaptic transmission, were evaluated. To confirm the potential neurotoxic effects of the treatment on the development of the central nervous system, both a transgenic <em>Tg(neuroD:gfp ia50)</em> line was used for confocal microscopy and Orange Acridine was used on wild type larvae to assess the presence of neuronal apoptosis. The results showed sub-lethal alterations, particularly affecting craniofacial and brain development highlighting as QXY may represent a possible endocrine disruptor able to induce severe cartilage defects, small head and tremor phenotype in zebrafish larvae and a strong modulation of the selected genes.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110252"},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of Qiukui Taoren Recipe against acute renal injury by inhibiting oxidative stress, inflammation and apoptotic pathways 秋葵桃仁方通过抑制氧化应激、炎症和凋亡通路对急性肾损伤的保护作用

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-16 DOI: 10.1016/j.cbpc.2025.110253

Jing Huang , Yuxin Wang , Lixin Feng , Meng Jin , Zhaoqing Meng , Xiaodi Dong , Jimiao Shan , Rongchun Wang , Kechun Liu

{"title":"Protective effect of Qiukui Taoren Recipe against acute renal injury by inhibiting oxidative stress, inflammation and apoptotic pathways","authors":"Jing Huang , Yuxin Wang , Lixin Feng , Meng Jin , Zhaoqing Meng , Xiaodi Dong , Jimiao Shan , Rongchun Wang , Kechun Liu","doi":"10.1016/j.cbpc.2025.110253","DOIUrl":"10.1016/j.cbpc.2025.110253","url":null,"abstract":"<div><div>Okra and peach kernel, named Qiukui and Taoren in China, respectively, which can be used for medicine and food, are widely applied for the treatment of multifaceted diseases. However, the protective effects against acute kidney injury (AKI) and the underlying mechanisms have not been fully assessed. The aim of this study was to assess the protective effect of Qiukui Taoren Recipe (QT), and the potential molecular mechanisms in AKI using network pharmacology prediction, <em>in vivo</em> and <em>in vitro</em> experiment. There were 42 active components of QT detected by HPLC-Q-TOF/MS, and network pharmacology showed that these active components mainly could act with 63 core targets such as TNF-α, Caspase-3, BCL2, STAT3, PPARγ, and regulated 110 signaling pathways for alleviating AKI. In addition, QT reduced periocular oedema, renal malformations and size reduction, partially restore renal excretory function, and reduced the accumulation of proteinuria in zebrafish AKI model induced by caristolochic acid (AA). QT could upregulate the mRNA expression of <em>Nephrin</em> and oxidative stress genes (<em>Nrf2</em>, <em>HO-1</em>, <em>PPARγ</em>), and downregulate inflammation- and apoptosis-related genes (<em>IL-1β</em>, <em>IL-6</em>, <em>TNF-α</em>, <em>NF-κB</em>, <em>Caspase-3</em>, <em>P53</em>). Furthermore, QT inhibited ROS elevation and apoptosis activated by cisplatin (CDDP) in HEK293T cells. Western blotting results showed that QT could enhance the protein expression of nephrin, and inhibited the protein expression of Kim-1, IL-18, NLRP3, STAT3, P53 and Caspase-3. Overall, these results indicated the protective effect of QT against AKI was due to the inhibition of oxidative stress, inflammation-and apoptosis-related pathways.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110253"},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of allelochemicals from three submerged plants and the inhibitory effects of organic acids on cyanobacteria (Cylindrospermopsis raciborskii) 3种沉水植物化感物质的鉴定及有机酸对蓝藻的抑制作用

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-15 DOI: 10.1016/j.cbpc.2025.110251

Junkai Zhang, Tinghui Min, Jinyu Wang, Zhikun Li, Xi Zhu, Shu-Xuan Liang

{"title":"Identification of allelochemicals from three submerged plants and the inhibitory effects of organic acids on cyanobacteria (Cylindrospermopsis raciborskii)","authors":"Junkai Zhang, Tinghui Min, Jinyu Wang, Zhikun Li, Xi Zhu, Shu-Xuan Liang","doi":"10.1016/j.cbpc.2025.110251","DOIUrl":"10.1016/j.cbpc.2025.110251","url":null,"abstract":"<div><div>C<em>yanobacteria</em> (<em>C. raciborskii</em>)<em>,</em> as a type of harmful algal bloom cyanobacteria, has strong invasiveness and has received widespread attention in eutrophic waters<em>.</em> Using allelopathic organic acids from submerged plants offers a promising strategy for controlling its blooms. This study investigated inhibitory effects of four plant-derived organic acids, including ellagic acid, gallic acid, caffeic acid, and p-hydroxybenzoic acid, on <em>C. raciborskii</em> through growth parameters, chlorophyll-a content, photosynthetic systems, and antioxidant responses<em>.</em> The results indicated that these organic acids significantly altered the concentrations of photosynthetic pigments, alkaline phosphatase (ALP) activity, and antioxidant enzyme activities of <em>C. raciborskii</em>. Different concentrations of organic acids had a significant influence on the intracellular photosynthetic pigment levels of <em>C. raciborskii</em>. These results revealed the reduction of chlorophyll-a was dose-dependent and time-dependent under the action of ellagic acid and gallic acid. All tested acids markedly elevated the alkaline phosphatase activity (248–398 % of the control group). Organic acids also significantly affected the activities of superoxide dismutase, catalase, and glutathione peroxidase, while increasing the level of malondialdehyde, indicating enhanced cellular oxidative stress. These findings suggest that organic acids inhibit photosynthetic pigment synthesis, phosphorus uptake, and antioxidative defenses, ultimately suppressing the growth of <em>C. raciborskii</em>.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110251"},"PeriodicalIF":3.9,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental and neurobehavioral toxicity of di-(2-ethylhexyl) phthalate (DEHP) in zebrafish larvae 邻苯二甲酸二（2-乙基己基）酯（DEHP）对斑马鱼幼虫的发育和神经行为毒性。

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-14 DOI: 10.1016/j.cbpc.2025.110254

Garima Jindal , Anuradha Mangla , Poonam Goswami , Mehjbeen Javed , Mohd. Anas Saifi , Iqra Mazahir , Padmshree Mudgal , Basir Ahmad , Sheikh Raisuddin

{"title":"Developmental and neurobehavioral toxicity of di-(2-ethylhexyl) phthalate (DEHP) in zebrafish larvae","authors":"Garima Jindal , Anuradha Mangla , Poonam Goswami , Mehjbeen Javed , Mohd. Anas Saifi , Iqra Mazahir , Padmshree Mudgal , Basir Ahmad , Sheikh Raisuddin","doi":"10.1016/j.cbpc.2025.110254","DOIUrl":"10.1016/j.cbpc.2025.110254","url":null,"abstract":"<div><div>Di-ethyl hexyl phthalate (DEHP), a plasticizer used in medical devices and cosmetics, easily leaches into the environment due to its weak chemical bonds with plastics. Humans and other organisms are exposed to DEHP through inhalation, ingestion, and dermal contact, inflicting toxic effects like neurotoxicity, hepatotoxicity, and reproductive toxicity. However, there is a lack of information on its developmental toxicity, and its role in demyelination is also not well understood. We explored the adverse effects of DEHP during the early developmental stage of zebrafish larvae, focusing on its deteriorative impact on the nervous system through demyelination at various concentrations (10, 20, 50, 100, and 200 ppm). Cuprizone was used as a positive control compound. Various markers of oxidative stress, hormonal changes, and altered expression of myelin-related protein were measured in larvae exposed to DEHP. Results showed that higher concentrations of DEHP had lethal effects on zebrafish larvae. The activity of acetylcholinesterase (AChE) and the level of reduced glutathione (GSH) were significantly decreased, while lipid peroxidation (LPO) and glutathione <em>S-</em>transferase (GST) activity were significantly increased. Additionally, cortisol and thyroxine levels were notably decreased, together with decreased mRNA expression of the myelin sheath and its cell markers such as myelin basic protein (<em>mbp</em>), SRY-box transcription factor (<em>sox10</em>), and oligodendrocyte lineage transcription factor 2 (<em>o</em><em>lig2</em>) in the DEHP-treated larvae. Similarly, protein expression of Mbp, Sox10, and Olig2 was also reduced. Overall, this study offers an understanding of the developmental neurotoxicity of DEHP and its behavioral and demyelinating effects in zebrafish larvae during early developmental stages.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110254"},"PeriodicalIF":3.9,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roundup Transorb reduces growth and expression of somatotropic axis genes in the silverside Odontesthes argentinesis fish larvae 农达Transorb降低阿根廷银齿鱼幼鱼的生长和促生长轴基因的表达

IF 3.9 3区环境科学与生态学

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2025-06-14 DOI: 10.1016/j.cbpc.2025.110256

Maidana Idiarte , Vinicius Farias Campos , Amanda Weege da Silveira Martins , Nivia Maria Streit , Gilson de Mendonça , Ricardo Berteaux Robaldo

{"title":"Roundup Transorb reduces growth and expression of somatotropic axis genes in the silverside Odontesthes argentinesis fish larvae","authors":"Maidana Idiarte , Vinicius Farias Campos , Amanda Weege da Silveira Martins , Nivia Maria Streit , Gilson de Mendonça , Ricardo Berteaux Robaldo","doi":"10.1016/j.cbpc.2025.110256","DOIUrl":"10.1016/j.cbpc.2025.110256","url":null,"abstract":"<div><div>Despite the use of herbicides has led to substantial increases in agricultural production, it has also introduced a series of environmental challenges that pose threats to the survival of non-target species in both terrestrial and aquatic ecosystems. Species of the genus <em>Odontesthes</em> have significant economic importance as they are valuable resources for artisanal fishing and local populations' income. Moreover, these organisms serve as crucial biological models in ecotoxicological and genetic research. Given the ecological and economic importance of <em>Odontesthes argentinensis</em>, this research aimed to investigate how the gene expression of the somatotropic axis (<em>gh, ghr1, ghr2, igf1,</em> and <em>igf2</em>) is impacted by the herbicide Roundup Transorb (RT), validating them as reliable biomarkers of exposure. <em>O. argentinensis</em> larvae were exposed to glyphosate at concentrations of 0.36, 1.8, and 3.6 mg/L for 96 h. The results indicate that there was a reduction in the expression of all analyzed genes in animals exposed to RT, resulting in decreased body growth. The expression of genes in the somatotropic axis was thus established as an effective biomarker for assessing exposure to Roundup Transorb herbicide.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"296 ","pages":"Article 110256"},"PeriodicalIF":3.9,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0