7-chloro-4-(phenylselanyl)quinoline exerts protective effect on acrylamide-induced neurotoxicity in the fly Drosophila melanogaster through modulation of acetylcholinesterase and oxidative stress

Abstract

This study aimed to evaluate the neuroprotective action of 7-chloro-4-(phenylselanyl)quinoline (4-PSQ) through the activity of the enzyme acetylcholinesterase (AChE), oxidative and behavioral parameters associated with acrylamide-induced neurotoxicity (ACR) in Drosophila melanogaster flies. The flies were divided into Control, 4-PSQ (25 μM), ACR (5 mM) and ACR + 4-PSQ (concomitantly) groups, and remained in treatments for 4 days. All analyses were performed with head samples. It was observed that flies belonging to the ACR + 4-PSQ group presented levels of reactive species and lipid peroxidation similar to the control, and similarly aligned the activity of the enzyme's superoxide dismutase, catalase and glutathione S-transferase, culminating in the preservation of mitochondrial and cellular metabolic capacity. There was a positive modulation of the activity of the AChE enzyme, which was attributed to the conservation of locomotor and exploratory behaviors, configuring the neuroprotective role of 4-PSQ. Together, these results may have reflected in the survival of the flies, which obtained a rate similar to the control. Considering the presence of ACR in the population's life, it was applied to the induction of a neurotoxicity model in Drosophila melanogaster, and thus, these results are scientifically relevant, as they conclude that 4-PSQ presented antioxidant and neuroprotective action.