Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"Single and combined effects of phenanthrene and cadmium on oxidative stress and detoxification related biomarkers in clams (Meretrix meretrix)","authors":"Yuanyuan Jing,&nbsp;Tianwen Zhang,&nbsp;Fanguang Hu,&nbsp;Guangbin Liu,&nbsp;Ming Sun","doi":"10.1016/j.cbpc.2024.110050","DOIUrl":"10.1016/j.cbpc.2024.110050","url":null,"abstract":"<div><div>Biomarkers concerning antioxidant reactions and detoxification metabolics were evaluated in <em>Meretrix meretrix</em> exposed to cadmium (Cd, 10 μg/L) and phenanthrene (PHE, 100 μg/L) individually and in combination (10 μg/L Cd + 100 μg/L PHE) for 7 days. The accumulation of Cd and PHE measured in the digestive gland, gill, mantle, and axe foot of the clam showed significant increase in combination treatment and it was higher than the single Cd or single PHE treatment. The activities of oxidative stress-related enzymes, the expression of <em>Cu</em>/<em>Zn SOD</em>, and the content of MDA increased after Cd and PHE exposure in the digestive gland and gill at most cases. In the digestive gland, <em>CAT</em> gene expression was significantly induced in Cd-single group and significantly inhibited in PHE-single group and Cd-PHE mixed group at both day 3 and day 7; in the gill, <em>CAT</em> gene expression was significantly inhibited in all groups at day 3 and except for Cd-single group at day 7. <em>MT</em> expression was significantly induced in Cd-single and Cd-PHE mixed groups at day 7, while <em>hsp70</em> expression was significantly inhibited in PHE-single and Cd-PHE mixed groups at day 7. The results indicated that SOD, CAT, GST, MDA, <em>Cu/Zn SOD</em>, <em>CAT</em>, <em>MT</em> and <em>hsp70</em> were sensitive to cadmium and PHE in a water environment, and can be used as indicators of marine heavy metal pollution.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110050"},"PeriodicalIF":3.9,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative biochemical characterization of pufferfish saxitoxin and tetrodotoxin-binding protein (PSTBP) homologs in the plasma from four Takifugu species: Conservation of heat-stable PSTBP orthologs having three and two tandemly repeated lipocalin domains in genus Takifugu","authors":"Yafei Zhang ,&nbsp;Mikinori Ueno ,&nbsp;Ryohei Tatsuno ,&nbsp;Tomohiro Takatani ,&nbsp;Yohei Shimasaki ,&nbsp;Kazunari Arima ,&nbsp;Mary Grace Sedanza ,&nbsp;Kenichi Yamaguchi ,&nbsp;Yuji Oshima ,&nbsp;Osamu Arakawa","doi":"10.1016/j.cbpc.2024.110049","DOIUrl":"10.1016/j.cbpc.2024.110049","url":null,"abstract":"<div><div>To study the relationship between domain characteristics of pufferfish saxitoxin and tetrodotoxin binding protein (PSTBP) proteoforms and their thermal stability, a comparative biochemical characterization of PSTBPs from the plasma of four <em>Takifugu</em> species (<em>T. flavipterus</em>, <em>T. pardalis</em>, <em>T. alboplumbeus</em> and <em>T. rubripes</em>) was conducted by Western blot analysis. The heat-tolerance tetrodotoxin (TTX)-binding ability of PSTBP proteoforms in <em>T. rubripes</em> plasma was verified by ultrafiltration and liquid chromatography tandem mass spectrometry (LC-MS/MS). These results suggest that the heat-stable PSTBP proteoforms, composed of three and two tandemly repeated lipocalin domains, are genetically conserved and ubiquitous in the genus <em>Takifugu</em>. This study builds on our knowledge of the structural and functional properties of PSTBP proteoforms, which is vital for understanding how toxins are transmitted and accumulate in organisms and is essential for evaluating the potential risks of toxins in seafood.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110049"},"PeriodicalIF":3.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability and expression patterns of housekeeping genes in Mediterranean mussels (Mytilus galloprovincialis) under field investigations","authors":"Letizia Iuffrida ,&nbsp;Rajapaksha Haddokara Gedara Rasika Wathsala ,&nbsp;Margherita Musella ,&nbsp;Giorgia Palladino ,&nbsp;Marco Candela ,&nbsp;Silvia Franzellitti","doi":"10.1016/j.cbpc.2024.110047","DOIUrl":"10.1016/j.cbpc.2024.110047","url":null,"abstract":"<div><div>The use of marine mussels as biological models encompasses a broad range of research fields, in which the application of RNA analyses disclosed novel biomarkers of environmental stress and investigated biochemical mechanisms of action. Quantitative real-time PCR (qPCR) is the gold standard for these studies, and despite its wide use and available protocols, it may be affected by technical flaws requiring reference gene data normalization. In this study, stability of housekeeping genes commonly employed as reference genes in qPCR analyses with <em>Mytilus galloprovincialis</em> was explored under field conditions. Mussels were collected from farms in the Northwestern Adriatic Sea. The sampling strategy considered latitudinal gradients of environmental parameters (proxied by location), gender, and their interactions with seasonality. Analyses of gene stability were performed using different algorithms. BestKeeper and geNorm agreed that combination of the ribosomal genes 18S ribosomal RNA (18S) and 28S ribosomal RNA (<em>28S)</em> was the best normalization strategy in the conditions tested, which agrees with available evidence. NormFinder provided different normalization strategies, involving combinations of tubulin (<em>TUB)/28S</em> (Gender/Season effect) or <em>TUB/</em>helicase <em>(HEL)</em> (Location/Season effect). Since NormFinder considers data grouping and computes both intra- and inter-group stability variations, it should work better with complex experimental designs and dataset structuring. Under the selected normalization strategies, expressions of the variable housekeeping genes actin (<em>ACT)</em> and elongation factor-1<em>α</em> (<em>EF1)</em> correlated with seasonal and latitudinal changes of abiotic environmental factors and mussel physiological status. Results point to consider <em>ACT</em> and <em>EF1</em> expressions as molecular biomarkers of mussel general physiological status in field studies.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110047"},"PeriodicalIF":3.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of 5α-reductase inhibition by dutasteride on reproductive gene expression and hormonal responses in zebrafish embryos","authors":"Hyunki Cho ,&nbsp;Indong Jun ,&nbsp;Karim Md Adnan ,&nbsp;Chang Gyun Park ,&nbsp;Sang-Ah Lee ,&nbsp;Juyong Yoon ,&nbsp;Chang Seon Ryu ,&nbsp;Young Jun Kim","doi":"10.1016/j.cbpc.2024.110048","DOIUrl":"10.1016/j.cbpc.2024.110048","url":null,"abstract":"<div><div>Steroid 5α-reductase (SRD5A) is a crucial enzyme involved in steroid metabolism, primarily converting testosterone to dihydrotestosterone (DHT). Dutasteride, an inhibitor of SRD5A types 1 and 2, is widely used for treating benign prostatic hyperplasia. An adverse outcome pathway (AOP) has been documented wherein SRD5A inhibition decreases DHT synthesis, leading to reduced levels of 17β-estradiol (E2) and vitellogenin (VTG), subsequently impairing fecundity in fish (AOP 289). However, the molecular and cellular mechanisms underlying these effects remain poorly understood. In this study, we assessed the impact of SRD5A inhibition on zebrafish embryos <em>(Danio rerio)</em>. Exposure to dutasteride resulted in decreased DHT, E2, and VTG levels, showing a positive correlation. Dutasteride also downregulated the expression of reproduction-related <em>genes (srd5a2, cyp19a1, esr1, esr2a, esr2b,</em> and <em>vtg</em>), with interrelated reductions observed across these levels. Docking studies suggested that dutasteride's effects may operate independently of androgen receptor (AR) and estrogen receptor (ER) interactions. Furthermore, co-exposure of dutasteride (0.5 or 2 μM) with 0.5 μM DHT revealed gene expression levels comparable to the control group. These findings underscore DHT's pivotal role in modulating estrogenic function and the interplay between estrogenic and androgenic responses in vertebrates. Our proposed AOP model offers insights into mechanistic gaps, thereby enhancing current understanding and bridging knowledge disparities.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110048"},"PeriodicalIF":3.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluoxetine antagonizes the acute response of LPS: Blocks K2P channels","authors":"Elizabeth R. Elliott,&nbsp;Robin L. Cooper","doi":"10.1016/j.cbpc.2024.110045","DOIUrl":"10.1016/j.cbpc.2024.110045","url":null,"abstract":"<div><div>The channels responsible for maintaining resting membrane potential are known as K2P (two-P-domain K⁺ subunit) channels, a subset of which are known to be blocked by Fluoxetine. In this experiment, the compound's effects on the membrane potential were examined on muscles in larval <em>Drosophila</em> overexpressing a subtype of K2P channel (known in <em>Drosophila</em> as dORKA1 or ORKA1) and compared to larvae without overexpression. The compound was also observed in sequence and/or combination with a form of lipopolysaccharide (LPS) that transiently activates K2P channels. Different concentrations of Fluoxetine were tested, and it was also examined in cocktail with the LPS. At 25 μM Fluoxetine exposure, muscle in control larvae underwent depolarization, while muscles overexpressing K2P channels hyperpolarized; at 50 μM, however, much more variable responses were observed. The LPS caused hyperpolarization in both larval strains, but the effect was more transient in the Canton-S line than in the K2P overexpressors. Finally, LPS continued to cause hyperpolarization even in the presence of Fluoxetine, while Fluoxetine quickly depolarized the muscle during exposure to LPS. The cocktail showed a smaller effect on muscles overexpressing ORKA1 as compared to the controls, indicating that Fluoxetine does not block the ORKA1 subtype. This study is significant because it demonstrates how overexpression of K2P channels alters membrane response to LPS and Fluoxetine exposure.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110045"},"PeriodicalIF":3.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of L-NAME and air exposure on mitochondrial energetic markers, thyroid hormone receptor/regulator system and stress/ease-responsive receptor expression in the brain/gut axis of zebrafish","authors":"Arathy Sudhakaran ,&nbsp;M.C. Subhash Peter","doi":"10.1016/j.cbpc.2024.110043","DOIUrl":"10.1016/j.cbpc.2024.110043","url":null,"abstract":"<div><div>As a signal molecule, nitric oxide (NO) has several physiological actions in fish. However, the action of NO on the brain/gut axis, a classic inter-organal axis that bridges the gastrointestinal tract and the CNS, still requires more understanding. The short-term <em>in vivo</em> action of a NO inhibitor, N-omega-nitro-L-arginine methyl ester (L-NAME), on mitochondrial energetic markers and the receptor expression of thyroid hormone (TH) and neuroendocrine hormones involved in stress/ease response was tested in the brain/gut axis of zebrafish exposed to either in non-stressed or air-exposed condition. L-NAME treatment decreased the NO content in brain and gut segments in non-stressed fish but rose upon L-NAME treatment in air-exposed fish that corresponded with the activation of inos, nnos, hif1a and hif1an transcript expressions. The brain/gut segments that showed spatial and differential sensitivity to L-NAME, modified the transcript expression patterns of stress (<em>adra2da</em>, <em>adrb1</em>, <em>nr3c2</em>)- and ease-responsive (<em>htr2b</em>, <em>slc6a4a</em>, <em>mtnr1aa</em>) hormone receptors. The expression pattern of the TH receptor/regulator system (<em>thra</em>, <em>thrb</em>, <em>dio1</em>, <em>dio2</em>, <em>dio3</em>) becomes more active in gut segments than brain segments upon L-NAME challenge in stressed zebrafish. The data provide evidence for a novel role of NO as an integrator of brain/gut axis segments in zebrafish, where the endogenously produced NO in mid-brain/posterior-gut axis aligns together upon air-exposure stress, providing a lead role to the posterior gut that activates and directs the neuroendocrine receptor expressions of stress/ease responsive genes. The data further invites studies exploring the therapeutic potential of L-NAME in this biomedical model to control the brain/gut axis segments.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110043"},"PeriodicalIF":3.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse effects of thimerosal on the early life stages of zebrafish","authors":"Lixia Li ,&nbsp;Kejun Dong ,&nbsp;LeYan Li ,&nbsp;Qingchen Li ,&nbsp;Youqin Su ,&nbsp;Chenrui Zong","doi":"10.1016/j.cbpc.2024.110046","DOIUrl":"10.1016/j.cbpc.2024.110046","url":null,"abstract":"<div><div>Thimerosal (THI) is an organic mercury compound that is widely used in drugs, vaccines and antibacterial products. Its extensive production and use have resulted in significant environmental contamination, posing a considerable threat to aquatic life. However, the knowledge of the toxicity of THI to aquatic organisms is still insufficient. In this study, we conducted a 5-day THI exposure experiment using zebrafish, from 0 to 5 days post fertilization (dpf). The possible adverse effects of THI on the early-life stages of zebrafish were explored by investigating variations in their physiological parameters, behavioral traits, and neurotransmitter levels. The results showed THI exhibited significant developmental toxicity to aquatic organisms. Exposure to THI significantly induced serious malformation (at 50 μg/L), accelerated hatching, and elevated heart rate (at 5 and 50 μg/L). The behavioral traits of zebrafish larvae had an increased first and then decreased relationship with increasing concentration of THI, which induced hyperactivity at 0.5 μg/L but opposite at 50 μg/L. Furthermore, exposure to 50 μg/L THI significantly raised levels of 5-HT, 5-HIAA, DA, DOPAC and ACH in zebrafish larvae. In addition, several significant correlations between behavioral traits and the neurotransmitter contents were detected, which seemed to reveal an important mechanism of the neurobehavioral toxicity of THI to fish.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110046"},"PeriodicalIF":3.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental toxicology of microplastic particles on fish: A review","authors":"Mahdi Banaee ,&nbsp;Cristiana Roberta Multisanti ,&nbsp;Federica Impellitteri ,&nbsp;Giuseppe Piccione ,&nbsp;Caterina Faggio","doi":"10.1016/j.cbpc.2024.110042","DOIUrl":"10.1016/j.cbpc.2024.110042","url":null,"abstract":"<div><div>The increase in plastic debris and its environmental impact has been a major concern for scientists. Physical destruction, chemical reactions, and microbial activity can degrade plastic waste into particles smaller than 5 mm, known as microplastics (MPs). MPs may eventually enter aquatic ecosystems through surface runoff. The accumulation of MPs in aquatic environments poses a potential threat to finfish, shellfish, and the ecological balance. This study investigated the effect of MP exposure on freshwater and marine fish. MPs could cause significant harm to fish, including physical damage, death, inflammation, oxidative stress, disruption of cell signalling and cellular biochemical processes, immune system suppression, genetic damage, and reduction in fish growth and reproduction rates. The activation of the detoxification system of fish exposed to MPs may be associated with the toxicity of MPs and chemical additives to plastic polymers. Furthermore, MPs can enhance the bioavailability of other xenobiotics, allowing these harmful substances to more easily enter and accumulate in fish. Accumulation of MPs and associated chemicals in fish can have adverse effects on the fish and humans who consume them, with these toxic substances magnifying as they move up the food chain. Changes in migration and reproduction patterns and disruptions in predator-prey relationships in fish exposed to MPs can significantly affect ecological dynamics. These interconnected changes can lead to cascading effects throughout aquatic ecosystems. Thus, implementing solutions like reducing plastic production, enhancing recycling efforts, using biodegradable materials, and improving waste management is essential to minimize plastic waste and its environmental impact.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110042"},"PeriodicalIF":3.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and toxicological characteristics of polyphenol oxidase from red palm weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)","authors":"Manal M.E. Ghanem,&nbsp;Ahmed M. Abd-Elaziz,&nbsp;Magda A. Mohamed","doi":"10.1016/j.cbpc.2024.110044","DOIUrl":"10.1016/j.cbpc.2024.110044","url":null,"abstract":"<div><div>Red palm weevil (RPW) <em>Rhynchophorus ferrugineus</em> is the most destructive insect pests of numerous palm species in the world. The introduction of botanical extract(s) as integral part of an integrated pest management (IPM) programs against RPW will reduce the use of chemical insecticides. Polyphenol oxidase (PPO) is one of the RPW innate immune mechanisms and inhibition of such enzyme could result in a disorder of the insect's immune system. A one single PO isoenzyme has been purified from the hemolymph of the 12th instar larvae of RPW. Using L-DOPA as substrate, <em>R. ferrugineus</em> PPO exhibited specific activity 428 Units/mg proteins with 8.3-fold purification, optimum pH and temperature for activity at 7.5 and 40 °C, respectively and is enhanced by Cu²⁺ with 1.76-fold. The rank order for oxidizing <em>R. ferrugineus</em> PPO different substrates is catechol &gt; pyrogallol &gt; L-DOPA &gt; pyrocatechuic acid and not tyrosine. The kinetic parameters Km, Vmax and Vmax/Km for L-DOPA are 3.3 mM, 1.3 μmol/ml/min, and 0.39, respectively. The catalytic efficiency of the enzyme towards catechol is 5.3-fold higher than that for L-DOPA. The enzyme completely inhibited by thiourea, ascorbic acid, dithiothreitol, and SDS. <em>R. ferrugineus</em> PPO is a catechol oxidase di-phenol: O₂ oxidoreductase. Based on the toxicological studies of various botanical extracts, the IC₅₀ ranged from 20 to 90 mg/ml. The enzyme completely inhibited by 50 mg/ml <em>Cinnamomum camphora</em>. Gallic acid, the major phenolic compound, has IC₅₀ 0.8 mM and competitively inhibited the enzyme with Ki 0.54 mM. <em>C. camphora</em> could be a useful natural RPW-controlling agent and used as integral part in IPM programs. This interpretation can be validated in future through an <em>in vivo</em> investigation.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110044"},"PeriodicalIF":3.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of exposure to 17α-methyltestosterone on hepatic lipid metabolism in Gobiocypris rarus","authors":"Tongyao Li ,&nbsp;Zijun Xiong ,&nbsp;Weiya Rong ,&nbsp;Qiong Yang ,&nbsp;Yue Chen ,&nbsp;Haiyan Zhao ,&nbsp;Qing Liu ,&nbsp;Jing Song ,&nbsp;Weiwei Wang ,&nbsp;Yu Liu ,&nbsp;Xianzong Wang ,&nbsp;Shaozhen Liu","doi":"10.1016/j.cbpc.2024.110041","DOIUrl":"10.1016/j.cbpc.2024.110041","url":null,"abstract":"<div><p>This study aimed to investigate the effects of 17α-Methyltestosterone (MT) on hepatic lipid metabolism in <em>Gobiocypris rarus</em>. <em>G. rarus</em> was exposed to varying concentrations of MT (0, 25, 50, and 100 ng/L) for durations of 7, 14, and 21 d. Biochemical and transcriptomic analyses were conducted using methods, such as ELISA, RT-qPCR, Western Blotting, and RNA-seq, to decipher the key signals and molecular mechanisms triggered by MT in vivo. The results revealed that MT induced hepatomegaly in <em>G. rarus</em> and markedly increased the hepatic steatosis index (HSI). After 14 d of exposure, significant increase in <em>PPARγ</em> mRNA expression was observed, whereas after 21 d, <em>PPARα</em> mRNA expression was significantly reduced. The expression pattern of <em>SREBP1C</em> mRNA initially decreased before increasing, mirroring the trend observed for SREBP1C protein expression. Furthermore, MT increased the levels of key lipid synthesis enzymes, including HSL, CPT1, GPAT, and FAS, thereby fostering lipid accumulation. RNA-seq analysis revealed that MT modulated hepatic bile acid metabolism via the PPAR pathway, consequently influencing cholesterol and lipid metabolism. Considering the differential metabolic pathways of MT across genders, it is postulated that MT may undergo aromatization to estrogen within <em>G. rarus</em>, thereby exerting estrogenic effects. These findings provide crucial experimental insights into the detrimental effects of MT in aquatic settings, underscoring its implications for safeguarding aquatic organisms and human health.</p></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"287 ","pages":"Article 110041"},"PeriodicalIF":3.9,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}