Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"Short-term exposure of enrofloxacin inhibits synthesis of central estradiol through secretoneurin a/aromatase B (cyp19a1b) pathway in crucian carp","authors":"Huazhong Liu ,&nbsp;Gen Wan ,&nbsp;Ping Luo ,&nbsp;Jianzhen Huang ,&nbsp;Jiayi Wu ,&nbsp;Zhuocheng Nie ,&nbsp;Jiming Ruan","doi":"10.1016/j.cbpc.2024.110112","DOIUrl":"10.1016/j.cbpc.2024.110112","url":null,"abstract":"<div><div>It has been reported that enrofloxacin (ENR) disrupts metabolic pathway of steroid in female crucian carp, promoting testosterone (T) synthesis through stimulating expression of luteinizing hormone (LH) and inhibiting conversion of T to estradiol (E₂) through repressing aromatase A expression. To further learn effect of ENR on steroid metabolism in fish, this work investigated effect of ENR on central E₂ synthesis and the involved mechanisms in female crucian carp through evaluating contents of T and E₂ in blood and brain, expression of secretogranin 2a (<em>scg2a</em>), gonadotrophin 2 β (<em>gth 2β</em>, namely LH) and aromatase B (<em>cyp19a1b</em>) genes in brain, and activation of PI3K/Akt pathway in brain of ENR exposed female crucian carp. Results revealed that ENR promoted steroid metabolism in brain of female crucian carp, stimulated synthesis of T synthesis but inhibited conversion of T to E₂ through promoting expression of <em>scg2a</em> and <em>gth 2β</em> but repressing expression of <em>cyp19a1b</em>, PI3K/Akt signaling pathway participated in regulating the biological process.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110112"},"PeriodicalIF":3.9,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocytosis, endoplasmic reticulum, actin cytoskeleton affected in tilapia liver under polystyrene microplastics and BDE153 acute co-exposure","authors":"Yao Zheng ,&nbsp;Jiajia Li ,&nbsp;Haojun Zhu ,&nbsp;Jiawen Hu,&nbsp;Yi Sun,&nbsp;Gangchun Xu","doi":"10.1016/j.cbpc.2024.110117","DOIUrl":"10.1016/j.cbpc.2024.110117","url":null,"abstract":"<div><div>Studies showed that contaminants adhered to the surface of nano-polystyrene microplastics (NPs) have a toxicological effect. Juveniles tilapia were dispersed into four groups: the control group A, 75 nm NPs exposed group B, 5 ng·L⁻¹ 2,2′,4,4′,5,5′-hexabromodiphenyl ether group C (BDE₁₅₃), and 5 ng·L⁻¹ BDE₁₅₃ + 75 nm MPs group D, and acutely exposed for 2, 4 and 8 days. The hepatic histopathological change, enzymatic activities, transcriptomics, and proteomics, have been performed in tilapia. The results showed that the enzymatic activities of anti-oxidative (ROS, SOD, EROD), energy (ATP), lipid metabolism (TC, TG, FAS, LPL, ACC), pro-inflammatory (TNFα, IL-1β) and apoptosis (caspase 3) significantly increased at 2 d in BDE₁₅₃ and the combined group and together in BDE₁₅₃ group at 8 d. Histological slice showed displaced nucleus by BDE₁₅₃ exposure and vacuoles appeared in the combined groups. KEGG results revealed that pathways associated with endocytosis, protein processing in endoplasmic reticulum and regulation of actin cytoskeleton were significantly enriched. The selected genes associated with neurocentral development (<em>ganab</em>, <em>diaph3</em>/<em>baiap2a</em>/<em>ddost</em> decreased and increased), lipid metabolism (<em>ldlrap1a</em> decreased, <em>stt3b</em> increased), energy (<em>agap2</em> decreased, <em>uggt1</em> increased) were affected under co-exposure, and fibronectin significantly increased via proteome. Our study indicated that endocytosis, protein processing in endoplasmic reticulum, regulation of actin cytoskeleton were affected in tilapia liver under NPs and BDE₁₅₃ co-exposure.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110117"},"PeriodicalIF":3.9,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-9a and miR-10482-5p regulate the expression of chitin synthase and chitinase genes, enhancing lufenuron tolerance in Spodoptera frugiperda","authors":"Shanshan Ling ,&nbsp;Zhimin Guo ,&nbsp;Mengyan Wu,&nbsp;Jiahui Tang,&nbsp;Haixiang Lv,&nbsp;Jianhong Li,&nbsp;Kangsheng Ma","doi":"10.1016/j.cbpc.2024.110115","DOIUrl":"10.1016/j.cbpc.2024.110115","url":null,"abstract":"<div><div><em>Spodoptera frugiperda</em> is a significant agricultural pest, severely impacting the yield and quality of grain. Chitin is the momentous component of exoskeletons, which has a significant impact on the growth and development of insects. Our previous study found that exposure to lufenuron can reduce the expression of chitinase gene (<em>SfCHT5</em>) and increase the expression of chitin synthase gene (<em>SfCHSB</em>), two key genes for chitin synthesis in <em>S. frugiperda</em>. However, the post-transcriptional regulatory mechanisms of these key genes in <em>S. frugiperda</em> remain unclear. With miRNA as the entry point, target site prediction, dual luciferase reporter assays, and mimics/inhibitors injection were used to explore the post-transcriptional regulatory mechanism of <em>SfCHSB</em> and <em>SfCHT5</em>. The combined results confirm that miR-10482-5p targets <em>SfCHT5</em> and regulates its expression, while miR-9a targets and regulates the expression of both <em>SfCHT5</em> and <em>SfCHSB</em>. Additionally, injection of miR-10482-5p and miR-9a mimic significantly reduced the mortality rates of <em>S. frugiperda</em> treated with lufenuron, increased pupation rate, and emergence rate. These findings underscore the critical regulatory role of miR-10482-5p and miR-9a in modulating the expression of key genes involved in chitin synthesis, thereby enhancing the tolerance of <em>S. frugiperda</em> to lufenuron and influencing its growth and development. In summary, this study preliminarily elucidates the post-transcriptional regulatory mechanisms of miRNA-mediated <em>SfCHSB</em> and <em>SfCHT5</em> expression.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110115"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms that regulate scopolamine effects on inducible fish antipredation responses in Daphnia magna","authors":"María Paula Carrillo,&nbsp;Benjamín Piña,&nbsp;Maria Vila-Costa,&nbsp;Carlos Barata","doi":"10.1016/j.cbpc.2024.110116","DOIUrl":"10.1016/j.cbpc.2024.110116","url":null,"abstract":"<div><div>Daphnia's antipredator responses are regulated largely by the nervous system, making these responses particularly susceptible to compounds that impact neurodevelopmental or neurofunctional processes. This study aimed to determine the molecular pathways involved in modulating the effects of scopolamine on inducible antipredation responses triggered by fish kairomones. We used two clones showing two contrasting responses. The positive phototactic clone 85 responds strongly to fish kairomones showing a marked negative phototactism and higher developmental rates. Consistently, the negative phototactic clone F shows the opposite behavior to the same stimuli. Adults of both clones were exposed to fish kairomones, scopolamine alone and a mixture of both. Scopolamine is a muscarine antagonist able to mimic fish kairomones inducible behavioral responses in both clones, while affecting differently morphological and life-history traits. Whole transcriptomic Illumina analyses indicated a greater number of de-regulated genes of the fish kairomone sensitive clone 85 (1650) compared to the F one (1138), which were grouped in four clusters (two per clone). The mixture of scopolamine and fish kairomone treatments on gene transcription was additive in both clones, indicating similar modes of action. Most enriched metabolic routes were related with neurological pathways and regulation of cell proliferation/differentiation. Our results indicate that fish kairomones and scopolamine deregulate not only neurological signaling pathways but also cell differentiation and proliferation pathways, which are linked to the observed behavioral responses as well as the developmental, morphological, and reproductive effects.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110116"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced uptake of perfluorooctanoic acid by polystyrene nanoparticles in Pacific oyster (Magallana gigas)","authors":"Arian Farajizadeh,&nbsp;Marina Giacomin,&nbsp;Greg Goss","doi":"10.1016/j.cbpc.2024.110119","DOIUrl":"10.1016/j.cbpc.2024.110119","url":null,"abstract":"<div><div>The effects of plastic pollution on marine organisms is of growing concern. The hydrophobic surface of plastics adsorbs organic contaminants and can alter the rate of chemical uptake in fishes. Per-fluorinated organic chemicals such as Perfluorooctanoic acid (PFOA) are highly hydrophobic toxic chemicals that adsorb to hydrophobic surfaces. We hypothesized that the presence of nano-sized plastic particles adsorbs PFOA and alter both the physical-chemical properties of the plastics and also enhance PFOA uptake into organisms. Using radiolabelled ¹⁴C-PFOA, we measured direct unidirectional uptake of PFOA in juvenile Pacific Oysters (<em>Magallana gigas</em>) at different (0.025, 0.50, and 0.100 mg/L) concentrations, for different exposure periods (1, 2, 4, and 6 h) and investigated whether varying concentrations (0.1, 0.5, 1 mg/L) of either 500 nm or 20 nm polystyrene nanoparticles (PS-NPs) differentially altered the uptake rate of PFOA. Our results demonstrate that PFOA adsorbs to the surface of PS-NPs, altering PS-NP behaviour in solution and significantly increases the rate of uptake of PFOA in exposed Pacific oysters. PFOA uptake at 0.1 mg/L was increased 2.3-fold in the presence of 1 mg/L 500 nm PS-NP and 3.2-fold in the presence of 1 mg/L 20 nm PS-NP. In a separate study to examine if PS NPs potentiate the biochemical response to PFOA, both 500 and 20 nm PS-NP at 100 mg/L increased the 1 mg/L PFOA-induced oxidative stress by 2.5-fold and 3-fold respectively. These findings demonstrate that nanoplastics as co-contaminants in marine systems are able to adsorb PFOA and significantly potentiate its uptake and toxicity.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110119"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of expanded polystyrene particle on energy metabolism of the sea slater (Ligia cinerascens) originating from a highly EPS-polluted area","authors":"Youn-Ha Lee ,&nbsp;Je-WonYoo ,&nbsp;Jee-Hyun Jung ,&nbsp;Young-Mi Lee","doi":"10.1016/j.cbpc.2024.110118","DOIUrl":"10.1016/j.cbpc.2024.110118","url":null,"abstract":"<div><div>Due to its high concentration and persistence, microplastic (MP) pollution is a major threat to marine environments. Expanded polystyrene (EPS) particles are the most abundant MP type in Asian regions, including the Korean coastal region. Although many previous studies have reported the toxicity of MPs to marine biota, the toxicity of environmentally relevant MPs to coastal organisms is not well understood. Thus, we investigated the toxicity of EPS on the growth and energy metabolism of the juvenile marine isopods, <em>Ligia cinerascens</em>, obtained from a population that has been exposed to EPS through multiple generations. After 14 and 21 days of dietary EPS exposure, body weight and molting of <em>L. cinerascens</em> were unaffected. However, the energy reserves (carbohydrates, proteins, and lipids) were significantly reduced, resulting in a decrease in the total energy budget (E_a) by dietary EPS exposure. The transcriptional modulation patterns of genes related to energy metabolism suggested that dietary EPS exposure may increase the digestion of non-carbohydrate sources, such as proteins and lipids, to compensate for increased energy expenditure. Our findings suggest that dietary EPS exposure, although no toxic at the individual level, can reduce the energy status of juvenile marine isopods, which provides useful information on the toxic effects of environmentally relevant MPs to coastal ecosystem.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110118"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity and immunomodulation of four novel cathelicidin genes isolated from the tiger frog Hoplobatrachus rugulosus","authors":"Danni Huang ,&nbsp;Fulong Gao ,&nbsp;Yixin Huang ,&nbsp;Ronghui Zheng ,&nbsp;Chao Fang ,&nbsp;Wenshu Huang ,&nbsp;Kejian Wang ,&nbsp;Jun Bo","doi":"10.1016/j.cbpc.2024.110091","DOIUrl":"10.1016/j.cbpc.2024.110091","url":null,"abstract":"<div><div>Cathelicidin is a family of antimicrobial peptides in vertebrates that plays an important role in resistance and immunization against pathogenic microorganisms. In the present study, the full-length cDNA sequences of four novel cathelicidins (<em>cathelicidin-1</em> to <em>cathelicidin-4</em>) in the tiger frog <em>Hoplobatrachus rugulosus</em>, encoding 153, 188, 132, and 160 amino acids, respectively, were firstly cloned by rapid amplification of the cDNA ends (RACE) technique. Sequence comparison and phylogenetic tree analysis indicated that the structures of the four cathelicidins are highly diverse. Afterwards, the tissue distribution profiles and antimicrobial patterns of cathelicidins in <em>H. rugulosus</em> were determined by real-time PCR. The four cathelicidins showed tissue-specific distribution patterns in the healthy frogs, and the transcriptional levels of cathelicidins exhibited a tissue- and time-dependency profile in the frogs challenged with pathogenic bacteria <em>Aeromonas hydrophila</em> for 72 h. The synthetic peptides of <em>cathelicidin-1</em> and <em>cathelicidin-2</em> exhibited broad-spectrum <em>in vitro</em> antimicrobial activity, and cathelicidins exerted antimicrobial activities through excessive induction of reactive oxygen species and direct disruption of the microbial membrane structure. In addition, the intraperitoneal injection of cathelicidin proteins significantly increased the marine medaka <em>Oryzias melastigma</em> resistance to bacterial challenges. The existence of multiple cathelicidins, their distinct tissue distribution patterns, and the inducible expression profiles suggest a sophisticated, highly redundant, and multilevel network of antimicrobial defense mechanisms in tiger frogs. This study provides evidence that cathelicidins have antimicrobial and immunomodulatory activities, and cathelicidins derived from <em>H. rugulosus</em> have potential therapeutic applications against pathogenic infections in aquaculture.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110091"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions of a PFOS/sodium nitrite mixture in Chinese mitten crab (Eriocheir sinensis): Impacts on survival, growth, behavior, energy metabolism and hepatopancreas transcriptome","authors":"Qiuran Yu ,&nbsp;Song Wang ,&nbsp;Erchao Li ,&nbsp;Yiwen Yang ,&nbsp;Xiaodan Wang ,&nbsp;Chuanjie Qin ,&nbsp;Jian G. Qin ,&nbsp;Liqiao Chen","doi":"10.1016/j.cbpc.2024.110114","DOIUrl":"10.1016/j.cbpc.2024.110114","url":null,"abstract":"<div><div>Perfluorooctanesulfonic acid (PFOS) and sodium nitrite may have complex adverse effects on aquatic animals. This study assessed the interactive effects of PFOS and sodium nitrite on Chinese mitten crab (<em>Eriocheir sinensis</em>). A 2 × 3 factorial experiment with 0, 0.1, and 5 mg/L PFOS and 0, 3.50 mg/L sodium nitrite evaluated impacts on growth, behavior, oxygen consumption, health, energy metabolism, and hepatopancreas transcriptome. PFOS &lt;0.1 mg/L with 3.50 mg/L nitrite significantly decreased PFOS accumulation in the hepatopancreas and improved feeding rates and hepatopancreas structure (<em>P</em> &lt; 0.05). Under 5 mg/L PFOS and nitrite, survival, weight gain, hepatosomatic index, and feeding rate significantly decreased (<em>P</em> &lt; 0.05). PFOS (0.1 mg/L) with nitrite significantly prolonged righting response time and increased locomotion speed (<em>P</em> &lt; 0.05). PFOS (5 mg/L) significantly decreased serum triglyceride and lactate levels, while PFOS and nitrite decreased glucose, triglyceride, and glycogen levels and increased lactate in hepatopancreas (<em>P</em> &lt; 0.05). Transcriptomic analysis indicated that PFOS affects p53 signaling, cell cycle and neurotransmission pathways, with notable changes in cell proliferation genes (<em>pcna</em>, <em>ccna</em>, <em>cdk1</em>, <em>cdk2</em>, <em>rbx1</em>) primarily downregulated by PFOS. Overall, PFOS disrupts neurotransmitter regulation and causes hepatopancreatic damage, while nitrite can reduce the toxicity of PFOS by decreasing its hepatopancreas accumulation. However, high levels of PFOS combined with sodium nitrite exacerbate toxicity, emphasizing the need for comprehensive assessment of environmental pollutant interactions.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110114"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of cyantraniliprole sublethal doses on the population dynamics and gene expression of Aphis gossypii Glover (Hemiptera: Aphididae)","authors":"Yuepeng Qiu ,&nbsp;Xianpeng Song ,&nbsp;Dan Wang ,&nbsp;Hongyan Hu ,&nbsp;Changcai Wu ,&nbsp;Yongpan Shan ,&nbsp;Yajie Ma ,&nbsp;Xiangliang Ren ,&nbsp;Yan Ma","doi":"10.1016/j.cbpc.2024.110113","DOIUrl":"10.1016/j.cbpc.2024.110113","url":null,"abstract":"<div><div>Cyantraniliprole (CYA), widely recognized as a highly effective solution, is widely used in pest management. It has been broadly utilized to manage diverse pests, among which <em>Aphis gossypii</em> Glover (Hemiptera: Aphididae) is a prominent agricultural pest that leads to significant crop damage worldwide. Studies suggest that the sublethal effect of insecticides might contribute to the resurgence of <em>A. gossypii</em>. Therefore, in this study, <em>A. gossypii</em> were exposed to sublethal doses of CYA (LC₁₅ and LC₃₀ values of 1.43 and 2.93 mg/L, respectively) for 48 h then employed life table parameters and RT-qPCR were used to estimate the sublethal and cross-generational impacts. Treatments with sublethal doses of CYA notably reduced the survival and reproduction of the F₀ <em>A. gossypii</em> and CYA at LC₃₀ significantly increased the fecundity and population growth parameters (<em>R</em>₀, <em>r</em>, <em>λ</em>, and <em>GRR</em>) of F₁ and reduced in the pre-adult stage. Furthermore, gene expression analysis indicated a significant downregulation of juvenile hormone-binding protein (<em>JHBP</em>) in F₀. Conversely, the F₁ generation exhibited an upregulation of vitellogenin (<em>Vg</em>), insulin receptor substrate 1 (<em>InS1</em>), ecdysone receptor (<em>EcR</em>), and ultraspiracle protein (<em>USP</em>). The funding not only enhance the comprehension of the sublethal effects of CYA on <em>A. gossypii</em> but also provide valuable guidance for the effective utilization of insecticides in managing the pest.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110113"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zearalenone delays tissue regeneration by dysregulating neutrophil balance in zebrafish (Danio rerio) larvae","authors":"Rui Luo ,&nbsp;Bo Wang ,&nbsp;Rui Luo","doi":"10.1016/j.cbpc.2024.110105","DOIUrl":"10.1016/j.cbpc.2024.110105","url":null,"abstract":"<div><div>Zearalenone (ZEA), a common mycotoxin, poses significant environmental and health risks. While its toxicological effects are well-studied, its impact on regeneration remains unclear. This study explored ZEA's effects on zebrafish (<em>Danio rerio</em>) larvae, focusing on developmental toxicity, immunotoxicity, and tissue regeneration. Embryos were exposed to 0, 0.5, 1, and 1.5 μM ZEA from 6 to 72 h post-fertilization (hpf). Although hatching and survival rates remained unaffected, malformations, including body axis bending and pericardial edema, increased dose-dependently, with 4.44 % abnormalities observed at 1.5 μM (<em>p</em> = 0.01). Heart rates also declined significantly at 1.5 μM (75.40 vs. 72.53 beats/30s, <em>p</em> = 0.0054). Immunotoxicity was assessed using Tg(mpx: eGFP) zebrafish to monitor neutrophil responses post-injury. ZEA exposure led to increased neutrophil counts (229.87 vs. 330.80, <em>p</em> &lt; 0.0001) and chemotaxis (21.15 % vs. 34.57 %, p &lt; 0.0001). RNA sequencing of 0 and 1.5 μM groups revealed disrupted redox balance and oxygen transport, with down-regulation of hbae1, hbbe2, and hbae3 and up-regulation of hif1a, indicating hypoxia involvement. Elevated reactive oxygen species (ROS), reduced antioxidant enzyme activity, and increased apoptosis were also observed.</div><div>Tail fin regeneration assays showed delayed regeneration at 1 and 1.5 μM ZEA, linked to impaired immune function and oxidative stress. These findings highlight ZEA's adverse effects on developmental and regenerative processes, underscoring its environmental and health implications and the need for further research.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110105"},"PeriodicalIF":3.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}