Open Medicine最新文献

{"title":"Impact of peripheral conditioning on reperfusion injury following primary percutaneous coronary intervention in diabetic and non-diabetic STEMI patients.","authors":"Veljko Andric, Radica Zivkovic Zaric, Dusan Andric, Jovan Petrovic, Goran Davidovic","doi":"10.1515/med-2025-1175","DOIUrl":"10.1515/med-2025-1175","url":null,"abstract":"<p><strong>Background: </strong>Peripheral conditioning induces transient ischemia, promoting antioxidant production in ischemia-affected tissues, which helps reduce heart reperfusion injury in ST-elevation myocardial infarction (STEMI) patients. This study compares troponin and creatine kinase-MB (CK-MB) levels among STEMI patients with and without remote conditioning.</p><p><strong>Methods: </strong>This study included 160 patients treated for STEMI at a tertiary care centre. The study protocol involved cyclic inflation and deflation of a blood pressure cuff on the brachial region in four cycles of 5 min each. Markers of myocardial necrosis, CK-MB, and troponin, were monitored before percutaneous coronary intervention (PCI), immediately after, and at 24, 48, and 72 h post-PCI.</p><p><strong>Results: </strong>CK-MB and troponin levels were significantly lower in non-diabetic patients who underwent remote peripheral conditioning compared to those who did not, with significant reductions observed after PCI (CK-MB: <i>p</i> = 0.001; troponin: <i>p</i> = 0.033), and at 24 (CK-MB: <i>p</i> = 0.015; troponin: <i>p</i> = 0.001) and 48 h post-PCI (troponin: <i>p</i> = 0.002). In the second phase, no significant differences in CK-MB or troponin levels were found between diabetic patients with and without conditioning. However, a trend toward lower values was noted in the conditioned group. In the third phase, significant reductions in CK-MB (<i>p</i> = 0.002) and troponin levels (after PCI: <i>p</i> = 0.007; 24 h post-PCI: <i>p</i> = 0.045) were observed across all patients who underwent conditioning compared to the control group.</p><p><strong>Conclusion: </strong>Peripheral pre- and post-conditioning is an economical, simple, and physiological method that effectively prevents and reduces heart damage caused by reperfusion injury, particularly in non-diabetic STEMI patients.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251175"},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RAB39B: A novel biomarker for acute myeloid leukemia identified via multi-omics and functional validation.","authors":"Shuo Chen, Mengxing Li, Jishi Wang","doi":"10.1515/med-2025-1168","DOIUrl":"10.1515/med-2025-1168","url":null,"abstract":"<p><strong>Background: </strong>The objective of this research was to investigate the involvement of RAB39B in acute myeloid leukemia (AML) using bioinformatics analysis and <i>in vitro</i> experiments for validation.</p><p><strong>Methods: </strong>In this article, RNA sequencing data from The Cancer Genome Atlas and genotype-tissue expression were utilized to analyze the expression of RAB39BA and identify differentially expressed genes.</p><p><strong>Results: </strong>AML exhibited elevated expression of RAB39B in diverse tumor types. In laboratory experiments, it has been demonstrated that RAB39B exhibits a significant expression level in AML cell lines when compared to normal peripheral blood monocytes. Moreover, RAB39B is closely linked to the growth and programmed cell death of AML cells.</p><p><strong>Conclusion: </strong>In conclusion, RAB39B shows potential as a biomarker for the identification and prediction of AML, contributing to the growth and cell death processes in AML.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251168"},"PeriodicalIF":1.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The safety and efficacy of BCG combined with mitomycin C compared with BCG monotherapy in patients with non-muscle-invasive bladder cancer: A systematic review and meta-analysis.","authors":"Jianping Liu, Weijian Zhou, Wei Zhang, Congwang Chang, Peng Zhang, Guanghua Fu","doi":"10.1515/med-2024-1134","DOIUrl":"10.1515/med-2024-1134","url":null,"abstract":"<p><strong>Introduction: </strong>We sought to determine the efficacy and safety of Bacillus Calmette-Guérin (BCG) combined with mitomycin C (MMC) compared with BCG monotherapy in intravesical therapies for non-muscle-invasive bladder cancer (NMIBC).</p><p><strong>Methods: </strong>We followed the recommended PRISMA guidelines for systematic reviews. Systematic literatures were performed on PubMed, EMBASE, Cochrane Library, CNKI, CBM, VIP, Wan Fang, and Clinical Trials.gov. Randomized controlled trials (RCTs) comparing BCG combined with MMC and BCG monotherapy in intravesical therapies for non-muscle-invasive bladder cancer patients were searched until August 1, 2023.</p><p><strong>Results: </strong>This meta-analysis included 11 RCTs with a total of 1,349 subjects. Compared with BCG monotherapy, BCG combined with MMC was associated with lower disease recurrence rate (relative risk [RR] 0.66, 95% confidence interval [CI]: 0.56-0.77, <i>P</i> < 0.00001), disease progression rate (RR 0.61, 95% CI: 0.44-0.84, <i>P</i> = 0.003), and disease-specific mortality (RR 0.46, 95% CI: 0.26-0.78, <i>P</i> = 0.004). However, there was a higher incidence of systemic adverse reactions (RR 1.57, 95% CI: 1.22-2.02, <i>P</i> = 0.0004). There was no significant difference in the incidence of local adverse reactions (RR 1.07, 95% CI: 0.95-1.20, <i>P</i> = 0.26) and all-cause mortality (RR 0.80, 95% CI: 0.62-1.03, <i>P</i> = 0.08) between the two groups.</p><p><strong>Conclusions: </strong>BCG combined with MMC was associated with a decreased risk of bladder cancer recurrence and disease progression compared with BCG monotherapy. However, there was no significant difference in the incidence of local adverse events and all-cause mortality between the two groups. Due to the limitations of the number and quality of the included studies, more high-quality RCTs are needed to further explore the efficacy and safety of combined therapies.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241134"},"PeriodicalIF":1.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of mRNA-modifying METTL3 oncogene as momentous prognostic biomarker responsible for colorectal cancer development.","authors":"Muhammad Naveed, Muhammad Saad Mughal, Tariq Aziz, Syeda Izma Makhdoom, Hamza Jamil, Ayaz Ali Khan, Nawal Al-Hoshani, Fakhria A Al-Joufi, Roaa Mohammed Tahir Kassim, Maher S Alwethaynani","doi":"10.1515/med-2025-1167","DOIUrl":"10.1515/med-2025-1167","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide, emphasizing the need for improved prognostic biomarkers. Recent studies have identified the mRNA-modifying METTL3 oncogene as a potential biomarker in CRC progression.</p><p><strong>Objective: </strong>This study aimed to investigate the expression patterns of METTL3 in CRC, assess its association with clinical outcomes, identify interacting proteins and biological pathways, and explore its correlation with immune cell infiltration.</p><p><strong>Methods: </strong>Comprehensive analyses were conducted using public datasets, including transcriptome profiles from The Cancer Genome Atlas and the GSE103512 dataset. Protein-protein interaction (PPI) networks, pathway enrichment, and immune infiltration analyses were performed to elucidate METTL3's role in CRC progression.</p><p><strong>Results: </strong>METTL3 expression was significantly higher in CRC tissues compared to normal tissues (<i>p</i> < 0.001). Mutations in METTL3 were detected in approximately 6% of CRC cases, with fusion events involving the SRPK2 gene. PPI analysis identified ten interacting proteins, including METTL4, EIF3H, RBM15B1, CBLL1, WTAP, NCBP1, RBM15, ZC3H13, METTL14, and KIAA1429. METTL3 expression showed a positive correlation with METTL4, METTL14, NCBP1, and WTAP expression (<i>R</i> > 0.5, <i>p</i> < 0.001). Higher METTL3 expression was associated with immunosuppressive phenotypes, such as increased infiltration of tumor-associated macrophages, regulatory T cells, and cancer-associated fibroblasts (<i>p</i> < 0.001). Pathway enrichment analysis revealed METTL3's involvement in crucial pathways, including the cell cycle and renal cell carcinoma (<i>p</i> < 0.01). Gene ontology analysis highlighted its role in mRNA and RNA-related processes.</p><p><strong>Conclusion: </strong>The study supports the potential of METTL3 as a prognostic biomarker in CRC and highlights its involvement in immune modulation and cancer progression. These findings lay the groundwork for future studies aimed at developing targeted therapies and improving patient outcomes.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251167"},"PeriodicalIF":1.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-dose preoperative intraperitoneal erythropoietin and intravenous methylprednisolone in acute traumatic spinal cord injuries following decompression surgeries.","authors":"Luowen Wang, Genbing Shi, Yongjia Jin, Zongyi Mo, Zhaogan Ren, Zhanqiang Hua","doi":"10.1515/med-2024-1105","DOIUrl":"10.1515/med-2024-1105","url":null,"abstract":"<p><strong>Background: </strong>Methylprednisolone is preferably used in acute traumatic spinal cord injuries but its efficacy is limited. The objectives of the study were to evaluate the efficacy and safety of preoperative intraperitoneal erythropoietin plus a high dose of methylprednisolone against a high dose of methylprednisolone monotherapy in patients with traumatic spinal cord injuries.</p><p><strong>Methods: </strong>In the retrospective study, patients received preoperative intraperitoneal erythropoietin + intravenous methylprednisolone (EM cohort, <i>n</i> = 107) or methylprednisolone monotherapy (PE cohort, <i>n</i> = 140).</p><p><strong>Results: </strong>The time between decompression surgeries and injuries was 34.58 ± 6.39 h/patient (maximum: 49 h). Neurologic and sphincter functions of patients at follow-up in the EM cohort exhibited better than the preoperative neurologic and sphincter functions in the same cohort and also neurologic and sphincter functions at follow-up in the PE cohort (<i>p</i> < 0.05 for all). Higher 30-day postoperative mortality was reported in the PE cohort (43 (31%) vs 20 (19%), <i>p</i> = 0.0454) than those of the EM cohort.</p><p><strong>Conclusions: </strong>Preoperative intraperitoneal erythropoietin plus a high dose of methylprednisolone appears to have a beneficial neuroprotective effect, exhibited improved sphincter functions, and decreased mortality more than a high dose of methylprednisolone monotherapy in patients with traumatic spinal cord injuries who underwent surgeries.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241105"},"PeriodicalIF":1.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the role of CXCL10 in pancreatic cancer progression: A novel prognostic indicator.","authors":"Xiaochao Wu, Longfei Rong, Ruiyi Tang, Quanpeng Li, Fei Wang, Xueting Deng, Lin Miao","doi":"10.1515/med-2024-1117","DOIUrl":"10.1515/med-2024-1117","url":null,"abstract":"<p><strong>Objective: </strong>Pancreatic cancer is distinguished by its high likelihood of metastasis and drug resistance, while the fundamental mechanisms are inadequately elucidated. This study aimed to identify pivotal hub genes associated with pancreatic cancer and assess their potential utility in predicting its onset and progression.</p><p><strong>Methods: </strong>Weighted gene co-expression network analysis (WGCNA) combined with differential expression analysis identified novel susceptibility modules and hub genes for pancreatic cancer. Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses were utilized to explore the potential roles of these hub genes. Receiver operator characteristic curves and nomogram models were developed to evaluate diagnostic efficacy. Mendelian randomization, flow cytometry, Transwell, and RNA sequencing were conducted to explore the association between C-X-C motif chemokine ligand 10 (CXCL10) and immune infiltration.</p><p><strong>Results: </strong>WGCNA analysis was performed to build gene co-expression networks, and ten key genes were found. CXCL10 was the central gene, and its expression was significantly linked to the survival of patients with pancreatic cancer and their response to immune checkpoint inhibitors. CXCL10 demonstrated the ability to stimulate the differentiation of macrophages toward the M2 phenotype. CXCL10 could facilitate the metastasis of pancreatic cancer cells by modulating macrophage polarization. CXCL10 affects macrophage polarization by regulating the expression of vascular endothelial growth factor A.</p><p><strong>Conclusions: </strong>CXCL10 demonstrates potential as a therapeutic target for managing pancreatic cancer.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241117"},"PeriodicalIF":1.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perform tumor-specific survival analysis for Merkel cell carcinoma patients undergoing surgical resection based on the SEER database by constructing a nomogram chart.","authors":"Jingxuan Zhou, Hai Yu, Xichun Xia, Yanan Chen, Wai-Kit Ming, Yuzhen Jiang, Yau Sun Lak, Chongchong Ip, Chaodi Huang, Qiqi Zhao, Suzheng Zheng, Liming Xia, Xinkai Zheng, Shi Wu, Jun Lyu, Liehua Deng","doi":"10.1515/med-2024-1103","DOIUrl":"10.1515/med-2024-1103","url":null,"abstract":"<p><strong>Objective: </strong>To explore the postoperative risk factors of Merkel cell carcinoma patients who have undergone surgical resection, and to construct a survival prognosis column chart.</p><p><strong>Method: </strong>Patients diagnosed with Merkel cell carcinoma and underwent surgical resection from 2000 to 2019 were selected from the surveillance, epidemiology, and end results database. COX regression analysis was used to screen for independent prognostic factors, and a column chart was constructed. The predictive performance of the column chart was evaluated using consistency index, receiver operating characteristic curve, and calibration curve.</p><p><strong>Results: </strong>The results of multi-factor COX regression showed that T stage and N stage were independent prognostic factors affecting cancer-specific survival (CSS) in patients after Merkel cell carcinoma resection. Construct a column chart based on the above two factors. The C-index of the column chart in the modeling group is 0.732 [95% CI (0.649, 0.814)], and the area under the curve (AUC) for the first and second years are 0.816 [95% CI (0.728, 0.904)] and 0.693 [95% CI (0.593, 0.792)], respectively. The C-index in the validation group was 0.724 [95% CI (0.569, 0.879)], and the AUC in the first and second years were 0.739 [95% CI (0.644, 0.833)] and 0.658 [95% CI (0.556, 0.759)], respectively.</p><p><strong>Conclusion: </strong>The predictive model constructed based on two factors, T stage and N stage, has good prognostic diagnostic accuracy and is helpful for clinical decision-making and personalized treatment.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241103"},"PeriodicalIF":1.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying early screening factors for depression in middle-aged and older adults: A cohort study.","authors":"Guanqun Chao, Lan Zhang, Zheli Zhan, Yang Bao","doi":"10.1515/med-2025-1160","DOIUrl":"10.1515/med-2025-1160","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to explore the current status of depression and related factors in middle-aged and elderly people in China using a cohort database with multi-year follow-up.</p><p><strong>Methods: </strong>The study population for this project was derived from the China Health and Retirement Longitudinal Study. Participants were divided into control and depression groups based on scores from the Center for Epidemiological Research Depression Scale. Continuous variables were compared using <i>t</i>-tests or Mann-Whitney U tests, while categorical variables were compared using chi-square tests. A multivariate logistic regression model was employed to evaluate factors associated with depression.</p><p><strong>Results: </strong>A total of 9,749 participants were included in the study. Correlation analyses revealed that age, body mass index, diastolic blood pressure, waist circumference, total cholesterol, uric acid, and length of sleep were significantly associated with depression (<i>p</i> < 0.05). Women exhibited a higher risk of depression in middle and old age compared to men (<i>p</i> < 0.05). An increase in waist circumference was associated with a decreased risk of depression (<i>p</i> < 0.05). Longer sleep duration and higher educational levels were also associated with a reduced risk of depression (<i>p</i> < 0.05). Unfavorable marital status and decreased frequency of alcohol consumption were found to increase the risk of depression (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>In middle-aged and elderly individuals, women, low education level, and experiencing dissatisfaction in marriage are connected with a high risk of depression. On the other hand, increased waist circumference, moderate alcohol consumption, and longer sleep duration are connected with a low risk of depression.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251160"},"PeriodicalIF":1.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative analysis of the impact of repeated administration of flavan 3-ol on brown, subcutaneous, and visceral adipose tissue.","authors":"Naomi Osakabe, Hitomi Nakamura, Yamato Yoshida, Sae Katsuragawa, Naoki Iida, Yasuyuki Fujii, Ursula M Jacob, Tilman Fritsch, Ali Abdelhameed, Vittorio Calabrese","doi":"10.1515/med-2025-1152","DOIUrl":"10.1515/med-2025-1152","url":null,"abstract":"<p><strong>Introduction: </strong>Flavan-3-ols (FLs), astringent polyphenols, are known to have low bioavailability and induce excessive sympathetic nervous system activation. This study aimed to compare the effects of FLs on brown, subcutaneous, and visceral adipose tissue in mice.</p><p><strong>Methods: </strong>C57BL/6J male mice fed a standard or high-fat diet were given water or 50 mg/kg FL orally by gavage for 2 weeks. Excised brown, inguinal, and epididymal fat tissues were prepared for frozen sectioning. After hematoxylin and eosin (HE) staining, the effects of FL administration on each adipose tissue were observed, and expression analysis of mitochondrial DNA genes was performed.</p><p><strong>Results: </strong>Repeated administration of FL had no morphological effects on brown adipose tissue or visceral fat. However, FL significantly reduced the cell size in subcutaneous fat and induced the appearance of multilocular structures. Furthermore, FL increased cytochrome B expression in subcutaneous fat. The results showed that FLs induce browning of subcutaneous fat in mice.</p><p><strong>Conclusion: </strong>This study showed that FL-induced enhancement of sympathetic nerve activity increased mitochondria in subcutaneous fat and promoted browning. However, no changes were observed in other adipose tissues. Further long-term administration is required to analyze the effects of FLs on adipose tissue thoroughly.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251152"},"PeriodicalIF":1.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrahydropalmatine improves mitochondrial function in vascular smooth muscle cells of atherosclerosis <i>in vitro</i> by inhibiting Ras homolog gene family A/Rho-associated protein kinase-1 signaling pathway.","authors":"Ke Ding, Qiying Bao, Jiaqi He, Jiahong Wang, Hui Wang","doi":"10.1515/med-2024-1059","DOIUrl":"10.1515/med-2024-1059","url":null,"abstract":"<p><strong>Background: </strong>Tetrahydropalmatine (THP) regulates mitochondrial function in vascular smooth muscle cells (VSMCs) to prevent or alleviate atherosclerosis (AS), with unclear specific mechanism.</p><p><strong>Methods: </strong>AS models were constructed by oxidized low-density lipoprotein (ox-LDL)-treated VSMCs. Cell counting kit-8 for cell viability, wound scratch assay for cell migration, and flow cytometry for cell cycle, intracellular reactive oxygen species, and mitochondrial membrane potential (MMP) were performed. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels by biochemical kits, oxygen consumption rate (OCR) by seahorse apparatus, apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay (TUNEL) staining, and apoptosis-related expression by western blot were detected. Ras homolog gene family A/Rho-associated protein kinase-1 (RhoA/ROCK1) levels were measured by western blot and ELISA. The RhoA agonist, U46619, was employed to validate mechanism of THP.</p><p><strong>Results: </strong>THP suppressed cell cycle progression and cell migration whereas alleviating cell viability and oxidative stress, as reduced MDA and enhanced SOD levels in ox-LDL-incubated VSMCs. THP protected mitochondrial function by higher MMP levels and OCR values. Additionally, THP decreased TUNEL-positive cells, Bax, Caspase-3, RhoA, ROCK1, and osteopontin expression, while increased Bcl-2 and smooth muscle myosin heavy chain levels. Furthermore, U46619 intervention antagonized effects of THP.</p><p><strong>Conclusion: </strong>THP improved mitochondrial function in VSMCs of AS by inhibiting RhoA/ROCK1 signaling pathway.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241059"},"PeriodicalIF":1.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}