Open Medicine最新文献_第2页

Inhibition of ATG7 promotes orthodontic tooth movement by regulating the RANKL/OPG ratio under compression force. 抑制ATG7通过调节压缩力作用下的RANKL/OPG比值促进正畸牙齿运动。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1252

Zhenjin Yang, Mingzhu Chen, Xianmin Liao, Liya Ma, Cun Liang, Yanjie Li, Jiangtian Hu

{"title":"Inhibition of ATG7 promotes orthodontic tooth movement by regulating the RANKL/OPG ratio under compression force.","authors":"Zhenjin Yang, Mingzhu Chen, Xianmin Liao, Liya Ma, Cun Liang, Yanjie Li, Jiangtian Hu","doi":"10.1515/med-2025-1252","DOIUrl":"10.1515/med-2025-1252","url":null,"abstract":"Objective: Autophagy serves as a protective mechanism in response to mechanical stress during OTM. However, the role of ATG7, a key regulatory gene in autophagy, in modulating periodontium remodeling during OTM remains unclear. This study aims to investigate the potential modulation of periodontium remodeling by ATG7 under compression force.Materials and methods: HPDLSCs and a rat OTM model were used as in vitro and in vivo systems to study the effect of compressive stress on autophagy and osteoclast-related markers. To investigate the role of ATG7, hPDLSCs with ATG7 knockdown and ATG7+/- rats were used in this study.Results: Compression force activates autophagy and increases the RANKL/OPG ratio. ATG7 knockdown significantly suppresses autophagy in hPDLSCs, while the RANKL/OPG ratio is markedly elevated. Under compression stress, hPDLSCs-siATG7 markedly enhanced RANKL/OPG expression. In the rat OTM model, autophagy was significantly activated in the periodontium on the compression side. Compared to wild-type SD rats, ATG7+/- rats exhibit reduced autophagy-related protein expression, an increased RANKL/OPG ratio, and accelerated tooth movement.Conclusions: Under compression force, inhibition of ATG7 expression significantly increases the RANKL/OPG ratio both in vivo and in vitro, which is accompanied by an increased rate of orthodontic tooth movement.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251252"},"PeriodicalIF":1.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk factors of frailty/sarcopenia in community older adults: Meta-analysis. 社区老年人虚弱/肌肉减少症的危险因素：荟萃分析

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1259

Xiaoyang Yan, Jiansong Zhou, Xia Cao

{"title":"Risk factors of frailty/sarcopenia in community older adults: Meta-analysis.","authors":"Xiaoyang Yan, Jiansong Zhou, Xia Cao","doi":"10.1515/med-2025-1259","DOIUrl":"10.1515/med-2025-1259","url":null,"abstract":"Objective: This study aimed to review the rates of physical frailty and sarcopenia, as well as associated risk factors, among adults aged 60 and older living in the community.Methods: A systematic literature search was conducted in PubMed, Wiley Library, Embase, Web of Science, and Cochrane Library for articles published from January 2000 to December 2024. The review followed PRISMA guidelines for meta-analysis.Results: Sixteen studies with 41,765 participants were included in the meta-analysis. The pooled prevalence of frailty/sarcopenia was 27% (95% CI: 19-35%). Specifically, the pooled prevalence of frailty was 25% (95% CI: 16-38%) and that of sarcopenia was 23% (95% CI: 13-37%). Age, malnutrition, depression, falls, and hypertension were identified as significant risk factors for frailty/sarcopenia.Conclusion: Nearly 30% of older adults in the community are affected by frailty/sarcopenia. Early identification of these risk factors is crucial for the prevention and management of frailty/sarcopenia. Further research is needed to determine effective interventions and strategies to reduce the incidence of frailty/sarcopenia.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251259"},"PeriodicalIF":1.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors determining the number of sessions in successful extracorporeal shock wave lithotripsy patients. 体外冲击波碎石术成功患者手术次数的决定因素。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-11 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1276

Müslüm Ergün, Süleyman Sağır

{"title":"Factors determining the number of sessions in successful extracorporeal shock wave lithotripsy patients.","authors":"Müslüm Ergün, Süleyman Sağır","doi":"10.1515/med-2025-1276","DOIUrl":"10.1515/med-2025-1276","url":null,"abstract":"Background and aim: The aim of this study is to investigate whether certain clinical nomograms influencing the success of extracorporeal shock wave lithotripsy (ESWL) also play a role in determining the number of treatment sessions required in patients who achieved stone-free status following ESWL.Methods: The data of 354 patients with successful ESWL outcomes were analyzed. Patients were evaluated 4-6 weeks post-ESWL using X-ray, ultrasound, or computed tomography. The presence of residual stones larger than 4 mm was considered a treatment failure. Data recorded included age, gender, laterality of stone localization (right/left), stone location (renal pelvis, mid calyx, upper calyx, proximal ureter, mid ureter, distal ureter), stone size (maximum longitudinal dimension), body mass index (BMI), and stone Hounsfield unit (HU) values. Patients were categorized into two groups: single-session and multiple-session treatment.Results: In the univariate model, factors such as age, BMI, distal ureter, renal pelvis, mid-calyx stone localization, stone size, and stone HU value showed a significant (p < 0.05) effect in distinguishing between single-session and multiple-session groups. Stone size and HU values were significantly higher (p < 0.05) in the multiple-session group compared to the single-session group. In the multivariate model, age, stone size, and HU value emerged as significant independent factors (p < 0.05) in differentiating between single-session and multiple-session treatments.Conclusion: Several factors influencing the success of ESWL also affect the number of sessions required. BMI, age, stone size, stone HU value, and certain stone locations are key determinants of the number of ESWL sessions.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251276"},"PeriodicalIF":1.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Procalcitonin and C-reactive protein as biomarkers for diagnosing and assessing the severity of acute cholecystitis. 降钙素原和c反应蛋白作为诊断和评估急性胆囊炎严重程度的生物标志物。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-11 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1258

Qisheng Hou, Huayu Li, Cheng Liu, Yujia Sun, Bo Wang, Hui Xiong, Si Liu

{"title":"Procalcitonin and C-reactive protein as biomarkers for diagnosing and assessing the severity of acute cholecystitis.","authors":"Qisheng Hou, Huayu Li, Cheng Liu, Yujia Sun, Bo Wang, Hui Xiong, Si Liu","doi":"10.1515/med-2025-1258","DOIUrl":"10.1515/med-2025-1258","url":null,"abstract":"Background: Acute cholecystitis (AC) is a common condition in emergency departments (EDs), where timely diagnosis and severity assessment are critical for treatment decisions and outcome prediction.Methods: This study included 194 patients with AC to evaluate the diagnostic and severity-grading performance of procalcitonin (PCT) and C-reactive protein (CRP). Receiver operating characteristic (ROC) curve analysis was used to determine optimal cut-off values for discriminating between severity grades according to the Tokyo Guidelines.Results: Elevated levels of PCT and CRP were observed in 73.2 and 67% of cases, respectively. PCT demonstrated a sensitivity of 75.6% and specificity of 64.8% at a cut-off value of ≤ 0.595 ng/mL for distinguishing grade I from grades II to III. For differentiating grade III from grades I to II, PCT showed a sensitivity of 62.5% and specificity of 92.1% at a cut-off value of ≥5.095 ng/mL. Similarly, CRP had a sensitivity of 82.9% and specificity of 64.8% at a cut-off value of ≤78 mg/L for grade I versus grades II to III, and a sensitivity of 62.5% and specificity of 69.7% at a cut-off value of ≥82.5 mg/L for grade III versus grades I to II.Conclusion: Both PCT and CRP are valuable biomarkers for diagnosing AC and assessing its severity.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251258"},"PeriodicalIF":1.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EsoFLIP-assisted dilation for dysphagia in systemic sclerosis: Highlighting the role of multimodal esophageal evaluation. esoflip辅助扩张治疗系统性硬化症患者的吞咽困难：强调多模式食管评估的作用。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-09 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1273

Subhankar Chakraborty, Martha Yearsley

{"title":"EsoFLIP-assisted dilation for dysphagia in systemic sclerosis: Highlighting the role of multimodal esophageal evaluation.","authors":"Subhankar Chakraborty, Martha Yearsley","doi":"10.1515/med-2025-1273","DOIUrl":"10.1515/med-2025-1273","url":null,"abstract":"Scleroderma is an autoimmune disorder characterized by vasculopathy, progressive skin fibrosis, and dysfunction of internal organs. Esophageal involvement is seen in most scleroderma patients. There is no specific treatment for dysphagia caused by scleroderma. FLIP technology is a novel diagnostic and therapeutic modality to assess the tightness of esophageal sphincters and esophageal motility and also provide therapeutic dilation. We describe the case of a patient with scleroderma who presented with dysphagia. Upper endoscopy was unremarkable. Esophageal manometry revealed aperistalsis with normal upper and lower esophageal sphincter (LES) relaxation. Barium esophagram demonstrated poor esophageal motility and hold-up of the barium tablet at the gastroesophageal junction. EndoFLIP test during sedated upper endoscopy revealed normal LES distensibility and absent peristalsis of the esophageal body. Due to dysphagia, the LES was dilated with a 30 mm EsoFLIP. A barium esophagram following the dilation revealed a significant reduction in esophageal body diameter, normalization of barium tablet transit, and symptomatic improvement in dysphagia. Our case is the first report of the use of EsoFLIP to help improve dysphagia in a scleroderma patient. It illustrates the importance of multi-modal functional testing in patients with dysphagia and suggests the utility of EsoFLIP in the treatment of dysphagia in these patients.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251273"},"PeriodicalIF":1.6,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Petunidin attenuates lipopolysaccharide-induced retinal microglia inflammatory response in diabetic retinopathy by targeting OGT/NF-κB/LCN2 axis. Petunidin通过靶向OGT/NF-κB/LCN2轴减轻脂多糖诱导的糖尿病视网膜病变视网膜小胶质细胞炎症反应。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-06 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1274

Mengxi Yu

{"title":"Petunidin attenuates lipopolysaccharide-induced retinal microglia inflammatory response in diabetic retinopathy by targeting OGT/NF-κB/LCN2 axis.","authors":"Mengxi Yu","doi":"10.1515/med-2025-1274","DOIUrl":"10.1515/med-2025-1274","url":null,"abstract":"Background: Diabetic retinopathy (DR), as a frequent complication of diabetes mellitus, is a common cause of vision impairment and blindness. Microglial activation plays an important role in the pathological cascade of DR, and novel potential therapeutics is needed to interfere with this process. Petunidin (PET) is one of the most abundant natural anthocyanins with the biological activities of anti-inflammation, anti-cancer, and anti-microbial.Objective: The purpose of this study is to investigate the effect of PET against lipopolysaccharide (LPS)-induced retinal microglia inflammatory response as well as the underpinning mechanism.Methods: Cell viability was determined using MTT assay. Cytokines secretion was determined using ELISA assay. iTRAQ-based proteomic analysis was used for the identification of differentially expressed proteins. Western blot analysis, Co-IP and immunofluorescence analysis were applied in the study.Results: The results showed that PET pre-treatment significantly reduced LPS-induced cytokines secretion in BV2 cells and primary retinal microglia, as well as lipocalin 2 (LCN2) upregulation in BV2 cells by suppressing activation of O-GlcNAc modification and activation of NF‑κB. Further study revealed that PET inactivated NF‑κB by down-regulating OGT in BV2 cells, indicating that the protective effect of PET against LPS-induced retinal microglia inflammatory response was achieved by regulating OGT/NF-κB/LCN2 axis.Conclusion: Our findings may contribute to the potential clinical use of PET in treating DR and suggest OGT/NF-κB/LCN2 axis may be the potential therapeutic target of this disease.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251274"},"PeriodicalIF":1.6,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lateral transperitoneal laparoscopic adrenalectomy: A single-centre experience of 21 procedures. 侧经腹膜腹腔镜肾上腺切除术：21例手术的单中心经验。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-06 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1230

Saman Anwar Wahid, Shakhawan Hama Amin Said, Lusan Abdulhameed Arkawazi, Mzhda Sahib Jaafar, Zhino Nuri Hussein, Attar Abdul Kareem Nadir

{"title":"Lateral transperitoneal laparoscopic adrenalectomy: A single-centre experience of 21 procedures.","authors":"Saman Anwar Wahid, Shakhawan Hama Amin Said, Lusan Abdulhameed Arkawazi, Mzhda Sahib Jaafar, Zhino Nuri Hussein, Attar Abdul Kareem Nadir","doi":"10.1515/med-2025-1230","DOIUrl":"10.1515/med-2025-1230","url":null,"abstract":"Background: Laparoscopic adrenalectomy (LA) is the gold standard technique for well-selected patients with an adrenal mass. The surgical team's experience is an essential factor in the surgical outcome.Objective: The aim of this study was to assess the outcomes of patients who underwent lateral transperitoneal LA to remove their adrenal mass.Methods: In this case-series study, 20 consecutive patients with adrenal mass were enrolled in Sulaimani Teaching Hospital, Sulaimaniyah, Iraq, from March 2019 to March 2022. Patients underwent lateral transperitoneal LA under general anaesthesia. Then, the type of LA, operation time, mass size, hospital stay, histopathological finding, and postoperative complications were assessed.Results: Most patients were females (65%), underwent left-sided LA (55%), had a nonfunctioning mass (55%), and had no postoperative complications (85%). The mean operation time was 95 ± 2.0 min, and the mean hospital stay was 3.2 ± 1.5 days. Most patients were detected incidentally through radiology (55%), while others were detected after biochemical tests were done, including cases of pheochromocytoma (25%), hypercortisolism (10%), and hyperaldosteronism (10%). Most patients had benign cortical adenoma (45%), followed by benign medullary pheochromocytoma (25%), myelolipoma (15%), oncocytoma, adrenal hyperplasia, and ganglioneuroma (5.0% each).Conclusions: LA seems to be a safe and feasible option for selected cases in this locality.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251230"},"PeriodicalIF":1.6,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological properties of valve materials using RGD and EC. 用RGD和EC研究阀门材料的生物特性。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1195

Chennian Xu, Yang Liu, Rui Qiao, Ping Jin, Hao Tang, Zhiyuan Tian, Bin Cui, Anguo Wen, Jian Yang

{"title":"Biological properties of valve materials using RGD and EC.","authors":"Chennian Xu, Yang Liu, Rui Qiao, Ping Jin, Hao Tang, Zhiyuan Tian, Bin Cui, Anguo Wen, Jian Yang","doi":"10.1515/med-2025-1195","DOIUrl":"10.1515/med-2025-1195","url":null,"abstract":"Background: Rapid application of transcatheter valve replacement for valve diseases has promoted the development of biological valves. Different efforts have been made to improve the surface properties of valves. We developed a new method using arginine-glycine-aspartate (RGD) peptide and epoxy chloropropane (EC). Our goal was to evaluate and detect the surface biological characteristics of valve materials using RGD and EC treatments.Methods: The surfaces of the valve materials used in the GA-EC and RGD-EC groups were smooth and relatively dense, as observed using a scanning electron microscope.Results: More MSCs adhered to and grew on the samples in the GA-EC and RGD-EC groups than in the GA group. The apoptosis rate of MSCs was markedly decreased, whereas the expression of vimentin was elevated in the GA-EC and RGD-EC groups (P < 0.05). The adherent ability of MSCs in the RGD-EC and GA-EC groups was significantly higher than that in the GA group (P < 0.05).Conclusions: The new treatment method using RGD and EC improves the biological properties of the surface of the biological valve materials.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251195"},"PeriodicalIF":1.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cholera epidemiology analysis through the experience of the 1973 Naples epidemic. 通过1973年那不勒斯疫情的经验分析霍乱流行病学。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1029

Bifulco Maurizio, Di Zazzo Erika, Pagano Cristina, Martini Mariano, Orsini Davide

{"title":"Cholera epidemiology analysis through the experience of the 1973 Naples epidemic.","authors":"Bifulco Maurizio, Di Zazzo Erika, Pagano Cristina, Martini Mariano, Orsini Davide","doi":"10.1515/med-2024-1029","DOIUrl":"10.1515/med-2024-1029","url":null,"abstract":"Background and objective: The World Health Organization (WHO) appeal of January 15, 2024, stated \"The current number, size and concurrence of multiple outbreaks, the spread to areas free of cholera for decades and alarmingly high mortality rates present a major threat to global health security.\" The current state is extremely worrying, considering the difficulties of countries in dealing with cholera epidemics due to the lack of funding and the difficulty in oral cholera vaccine production and administration. This study aims to analyse the past and current influence of anthropization on cholera onset.Methods: We analysed the literature, particularly of the last 5 years, on the influence of human actions that impact the spread of cholera.Results: The epidemiological data published by WHO and the available literature highlight a strong impact of human actions on the epidemic spread of cholera, the government's difficulty in making decisions on epidemic prevention or containment, and the fear of the population.Conclusions: Cholera should be considered an anthropogenic disaster, considering the historical health analysis of the cholera epidemics in Italy in the last two centuries and in southern Italy and in Naples in 1973.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241029"},"PeriodicalIF":1.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endotoxin tolerance inhibits NLRP3 inflammasome activation in macrophages of septic mice by restoring autophagic flux through TRIM26. 内毒素耐受通过TRIM26恢复自噬通量，抑制脓毒症小鼠巨噬细胞NLRP3炎性体活化。

IF 1.6 4区医学

Open Medicine Pub Date : 2025-09-03 eCollection Date: 2025-01-01 DOI: 10.1515/med-2025-1231

Yanyan Yang, Shiwen Wu, Minghao Lin, Xueting Xie, Huifang Shi, Youran Chen, Shanshan Li, Yuchun Jiang, Sijie Zheng, Chibin Shen, Naibin Yang, Mingqin Lu

{"title":"Endotoxin tolerance inhibits NLRP3 inflammasome activation in macrophages of septic mice by restoring autophagic flux through TRIM26.","authors":"Yanyan Yang, Shiwen Wu, Minghao Lin, Xueting Xie, Huifang Shi, Youran Chen, Shanshan Li, Yuchun Jiang, Sijie Zheng, Chibin Shen, Naibin Yang, Mingqin Lu","doi":"10.1515/med-2025-1231","DOIUrl":"10.1515/med-2025-1231","url":null,"abstract":"Objective: Endotoxin tolerance (ET) has been demonstrated to attenuate the inflammatory response in murine models of sepsis. This study seeks to elucidate the underlying mechanisms by which ET modulates inflammation in sepsis, with a particular focus on macrophage autophagy.Methods: An in vivo sepsis model was generated using cecal ligation and perforation, while an in vitro model of inflammatory injury was induced via lipopolysaccharide (LPS) administration. ET was established through pretreatment with low-dose LPS. Subsequent analyses were conducted to assess the presence of the NLRP3 inflammasome, autophagic flux, and the expression levels of TRIM26.Results: Heightened inflammation was observed in the TNF-α levels and various organs of the sepsis group; conversely, inflammation was reduced in the group receiving ET treatment. Upon stimulation with LPS, primary mouse peritoneal macrophages exhibited activation of the NLRP3 inflammasome and autophagy, accumulation of mitochondrial reactive oxygen species, compromised membrane potential, resulting in cell apoptosis, and decreased expression of TRIM26. ET was found to enhance autophagy, suppress the activation of NLRP3 inflammasomes, and upregulate the expression of TRIM26. Interestingly, modulation of autophagy levels either reversed or intensified the protective effects of ET on macrophages in vitro. Knockdown of TRIM26 using small interfering RNA (siRNA) resulted in increased NLRP3 inflammasome activation and accumulation of P62.Conclusion: We reveal that ET restores the autophagic flux in macrophages, inhibit NLRP3 inflammasome activation, and mitigate inflammatory damage in septic mice, potentially through the regulation of TRIM26.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20251231"},"PeriodicalIF":1.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0