Open Medicine最新文献

Comparing the therapeutic efficacy of endoscopic minimally invasive surgery and traditional surgery for early-stage breast cancer: A meta-analysis.

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-20 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1133

Qiyi Ma, Tingting Shi, Huan Wang, Jie Xing

{"title":"Comparing the therapeutic efficacy of endoscopic minimally invasive surgery and traditional surgery for early-stage breast cancer: A meta-analysis.","authors":"Qiyi Ma, Tingting Shi, Huan Wang, Jie Xing","doi":"10.1515/med-2024-1133","DOIUrl":"10.1515/med-2024-1133","url":null,"abstract":"Background: Early-stage breast cancer requires effective surgical interventions. This meta-analysis compares the therapeutic efficacy of endoscopic minimally invasive surgery (EMIS) with traditional surgery, such as modified radical mastectomy.Methods: Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and the Patient, Intervention, Comparison, Outcome model, we systematically searched PubMed, Embase, Web of Science, and the Cochrane Library until July 19, 2023. We included comparative trials, focusing on randomized controlled trials, retrospective, and prospective studies. Metrics analyzed included operative time, blood loss, postoperative drainage volume, and lymph node harvest using Stata version 17.Results: Out of 943 studies, six met the inclusion criteria. Endoscopic surgery had a longer operative time (weighted mean difference [WMD] = 1.03, P < 0.01) but significantly less blood loss (WMD = -1.48, P < 0.01). No significant differences were noted in drainage volume and lymph node harvest.Conclusions: EMIS reduces intraoperative blood loss but requires more time than traditional surgeries. Both methods show comparable outcomes in postoperative drainage and lymph node harvest, supporting their efficacy in treating early-stage breast cancer.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241133"},"PeriodicalIF":1.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the effectiveness of nurse-led preoperative counseling and postoperative follow-up care vs standard care for patients with gastric cancer. 护士主导的胃癌患者术前咨询及术后随访与标准护理的效果比较。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1098

Wenwen Wang, Yan Zhu, Yu Sun, Yandong Li

{"title":"Comparison of the effectiveness of nurse-led preoperative counseling and postoperative follow-up care vs standard care for patients with gastric cancer.","authors":"Wenwen Wang, Yan Zhu, Yu Sun, Yandong Li","doi":"10.1515/med-2024-1098","DOIUrl":"10.1515/med-2024-1098","url":null,"abstract":"Background: Radical gastrectomy is generally prefered for gastric cancer but has postoperative complications. The objectives of the study are to evaluate the effectiveness of three different models of preoperative counseling and postoperative follow-up care in patients who underwent radical gastrectomy for gastric cancer.Methods: In retrospective medical record analyses, patients received nurse-led preoperative counseling and postoperative follow-up care (NC cohort, n = 105) or surgeon-led preoperative counseling and surgeon-led follow-up (SC cohort, n = 140), or did not receive counseling and aftercare (RC cohort, n = 160).Results: Patients had a postoperative intensive care unit (PICU) stay of 24 (26-22) h/patient and postoperative pain of 6 (6-5)/ patient. The frequencies of nausea, vomiting, medical intensive care unit admission(s), dizziness, length of PICU stay, and intensity of postoperative pain were lower among patients in the NC cohort, followed by those in the SC and RC cohorts. A higher number of patients in the RC cohort died when compared to the NC (25 [16%) vs 2 [2%], p = 0.0458) and the SC (25 [16%] vs 11 [8%], p = 0.0001) cohorts in follow-up.Conclusion: Patients who undergo radical gastrectomy for gastric cancer require healthcare professional-led preoperative counseling and postoperative aftercare. Nurse-led preoperative counseling and postoperative aftercare, could improve outcome measures in patients who underwent radical gastrectomy for gastric cancer.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241098"},"PeriodicalIF":1.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiangiogenic potential of Elaeagnus umbellata extracts and molecular docking study by targeting VEGFR-2 pathway. 黑叶参提取物抗血管生成潜能及靶向VEGFR-2途径的分子对接研究

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1083

Nausheen Nazir, Arbaz Waqar, Amir Zaib Khan, Ayaz Ali Khan, Tariq Aziz, Abdullah F Alasmari

{"title":"Antiangiogenic potential of Elaeagnus umbellata extracts and molecular docking study by targeting VEGFR-2 pathway.","authors":"Nausheen Nazir, Arbaz Waqar, Amir Zaib Khan, Ayaz Ali Khan, Tariq Aziz, Abdullah F Alasmari","doi":"10.1515/med-2024-1083","DOIUrl":"10.1515/med-2024-1083","url":null,"abstract":"Background: Anti-angiogenesis or inhibition of blood vessel formation is the best way to prevent the growth and metastasis of tumors. Natural sources like plants are currently being explored for its antiangiogenic activity as they are factories of various phytochemicals. The goal of the current study is to investigate the antiangiogenic potential of Elaeagnus umbellata (E. umbellata) by using chorioallantoic membrane (CAM) assay and molecular docking.Methods: Based on our previous research, the antiangiogenic activity was carried out using active fractions including crude methanol (Eu-Met), ethyl acetate (Eu-EtAc), and chloroform (Eu-Chf) extracts using CAM assay. Furthermore, to understand the binding mechanism of identified compounds, molecular docking was performed against vascular endothelial growth factor receptor 2 (VEGFR-2) using AutoDock vina as docking software. VEGFR-2 is overexpressed in pathological angiogenesis.Results: In CAM assay, Eu-Met, Eu-EtAc, and Eu-Chf extracts showed antiangiogenic activities but notable antiangiogenic activities were exhibited by Eu-Chf with IC50 value of 65.02 μg/mL. In molecular docking, five compounds, catechin, catechin hydrate, morin, quercetin, and rutin, reported in the extract and active fractions (Eu-Met, Eu-EtAc, and Eu-Chf) of E. umbellata showed strong interactions with VEGFR-2 with binding affinities of -9.4, -9.3, -9.9, -10.2, and -9.4 kcal/mol.Conclusion: Based on our results, we can claim that E. umbellata possess antiangiogenic activity which needs to be explored further.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241083"},"PeriodicalIF":1.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CMSP exerts anti-tumor effects on small cell lung cancer cells by inducing mitochondrial dysfunction and ferroptosis. CMSP通过诱导线粒体功能障碍和铁下垂对小细胞肺癌细胞有抗肿瘤作用。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-15 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1100

Xi Yan, Yinghao Niu, Yaojie Wang, Sisi Wei, Lina Han, Zhongyu Guo, Lianmei Zhao, Feng Gao

{"title":"CMSP exerts anti-tumor effects on small cell lung cancer cells by inducing mitochondrial dysfunction and ferroptosis.","authors":"Xi Yan, Yinghao Niu, Yaojie Wang, Sisi Wei, Lina Han, Zhongyu Guo, Lianmei Zhao, Feng Gao","doi":"10.1515/med-2024-1100","DOIUrl":"https://doi.org/10.1515/med-2024-1100","url":null,"abstract":"Purpose: This study aims to investigate the role and mechanism of p-hydroxyl cinnamaldehyde (CMSP) in triggering ferroptosis of small cell lung cancer (SCLC) cells.Methods: The impact of CMSP on ferroptosis in H1688 and SW1271 cells was assessed through cell experiments and biological information analysis. Moreover, the expression of heme oxygenase 1 (HMOX1) in SCLC tissue was examined.Results: Following CMSP treatment, a concentration-dependent increase in cell death was observed, and differentially expressed genes were found to be associated with ferroptosis. CMSP notably facilitated ferroptosis events, such as elevated levels of reactive oxygen species (ROS), Fe2+, malondialdehyde (MDA), transferrin receptor 1 (TFR1), divalent metal transporter 1 (DMT1), and decreased levels of glutathione (GSH), solute carrier family 7 member 11 (SLC7A11), and glutathione peroxidase 4 (GPX4). Furthermore, CMSP promoted mitochondrial dysfunction, manifested as reduced mitochondrial volume, increased membrane density, elevated mitochondrial ROS, and decreased mitochondrial membrane potential. Consistently, the mitochondrial-targeted antioxidant Mito-TEMPO reversed CMSP-induced ferroptosis. Expression of the HMOX1 gene was markedly increased under CMSP treatment, while lower expression was observed in cancer tissue compared to adjacent tissue.Conclusion: CMSP triggers mitochondrial dysfunction via HMOX1 activation, leading to ferroptosis in SCLC cells, underscoring its potential as a therapeutic agent for SCLC.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241100"},"PeriodicalIF":1.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive value of plasma sB7-H3 and YKL-40 in pediatric refractory Mycoplasma pneumoniae pneumonia. 血浆sB7-H3和YKL-40对小儿难治性肺炎支原体肺炎的预测价值。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-15 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1114

QiuMin Zhao, ShiYan Ji, HaiPing Jiang, DongMing Lu, LiFen Qian, JingWen Zhang, Yue Cui, Wei Lin, HaoYing Ge, Meng Gu

{"title":"Predictive value of plasma sB7-H3 and YKL-40 in pediatric refractory Mycoplasma pneumoniae pneumonia.","authors":"QiuMin Zhao, ShiYan Ji, HaiPing Jiang, DongMing Lu, LiFen Qian, JingWen Zhang, Yue Cui, Wei Lin, HaoYing Ge, Meng Gu","doi":"10.1515/med-2024-1114","DOIUrl":"https://doi.org/10.1515/med-2024-1114","url":null,"abstract":"Objective: This study investigated the clinical significance of plasma sB7-H3 and YKL-40 levels in children with refractory Mycoplasma pneumoniae pneumonia (RMPP).Methods: A total of 182 RMPP patients (103 general Mycoplasma pneumoniae patients and 79 RMPP patients) were included. sB7-H3, YKL-40, and other inflammatory factors were measured. Independent factors associated with the early diagnosis of RMPP were determined. The value of each independent risk factor in predicting RMPP was evaluated.Results: The RMPP group reported significantly longer hospital stays and total fever durations. Levels of C-reactive protein, D-dimer, IL-13, IL-6/-10, sB7-H3, and YKL-40 were higher in the RMPP group. sB7-H3 was positively correlated with IL-13, IL-6, and IL-4, whereas YKL-40 was positively correlated with all of the above indicators (IL-5 was also included). sB7-H3 and YKL-40 were independent risk factors for RMPP. The critical values for sB7-H3 and YKL-40 were 3.525 and 313.3 ng/mL, respectively. sB7-H3 and YKL-40 had areas under the curve of 0.734 and 0.859, respectively. YKL-40 had high sensitivity and specificity of 88.61 and 87.38%, respectively. Both indicators had predictive value, YKL-40 had the highest predictive ability for RMPP.Conclusion: Detection of sB7-H3 and YKL-40 levels in the plasma may be useful in diagnosing RMPP early in the disease process.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241114"},"PeriodicalIF":1.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of headspace gas chromatography with a flame ionization detector method for the determination of ethanol in the vitreous humor. 用火焰电离检测器顶空气相色谱法测定玻璃体中乙醇的方法的建立和验证。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-15 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1123

Filip Mihajlović, Ivana Andrić, Živana Slović, Maja Vujović, Kristina Piskulić, Snežana Đorđević

{"title":"Development and validation of headspace gas chromatography with a flame ionization detector method for the determination of ethanol in the vitreous humor.","authors":"Filip Mihajlović, Ivana Andrić, Živana Slović, Maja Vujović, Kristina Piskulić, Snežana Đorđević","doi":"10.1515/med-2024-1123","DOIUrl":"10.1515/med-2024-1123","url":null,"abstract":"Introduction: Qualitative and quantitative testing of ethanol in post-mortem samples is an important analytical procedure that provides accurate, precise, and reliable results. Given the complexity of the issue, obtaining a realistic picture of lifelong alcoholemia requires supporting blood ethanol findings with analyses of alternative samples, primarily vitreous humor (VH).Objective: The objective of this study was to develop and validate a headspace gas chromatography with flame ionization detection (HS/GC-FID) method for determining ethanol concentration in VH.Materials and methods: Conditions for the HS/GC-FID method were established and the method was validated according to the guidelines of the European Medicines Agency. Validation parameters such as precision, accuracy, specificity, sensitivity, and linearity over a wide concentration range were evaluated through statistical analysis.Results: The method demonstrated precision, accuracy, specificity, and sensitivity. Additionally, it proved to be linear across a wide concentration range and relatively fast, making it suitable for rapid and routine determination of ethanol concentration in VH, particularly for forensic applications.Conclusion: Results from validation and application of the method to VH samples indicate that ethanol concentration in VH can be reliably determined using the presented HS/GC-FID method, making it a valuable tool in forensic investigations.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241123"},"PeriodicalIF":1.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway. CCNB1的下调通过Wnt/β-catenin信号通路缓解妊娠期糖尿病中高糖引发的滋养细胞功能障碍。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1119

Biru Xiao, Wenmiao Zhang, Nini Ji, Qiuyue Chen

{"title":"Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway.","authors":"Biru Xiao, Wenmiao Zhang, Nini Ji, Qiuyue Chen","doi":"10.1515/med-2024-1119","DOIUrl":"10.1515/med-2024-1119","url":null,"abstract":"Gestational diabetes mellitus (GDM), defined as glucose intolerance occurring or first detected during pregnancy, affects approximately 8% of pregnancies worldwide. The dysfunction of trophoblasts in pregnancies complicated by GDM is associated with changes in trophoblast cell functions, resulting in compromised proliferation and regulation of the cell cycle. Cyclin B1 (CCNB1), a pivotal controller of the start of mitosis, is crucial in these mechanisms. Nevertheless, the precise function of CCNB1 in trophoblast dysfunction related to GDM has not been extensively investigated. The aim of this study was to investigate CCNB1's role in high glucose (HG)-triggered trophoblast. Herein, we revealed that in HG-stimulated HTR8/SVneo cells, CCNB1 is highly expressed. Knockdown of CCNB1 significantly promotes the growth of HG-stimulated HTR8/SVneo cells and suppresses inflammation (p < 0.05). Additionally, reducing CCNB1 expression significantly improves glucose uptake and inhibits the Wnt/β-catenin pathway in HG-stimulated HTR8/SVneo cells (p < 0.05). In conclusion, our study demonstrated that the deletion of CCNB1 can alleviate trophoblast dysfunction induced by HG in GDM through the Wnt/β-catenin pathway. This suggests that CCNB1 may be a potential target for managing GDM. Although our results underscore the potential therapeutic benefits of reducing CCNB1 in mitigating trophoblast dysfunction, it is important to note that the study is limited by its reliance on a single cell line and the absence of in vivo validation.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241119"},"PeriodicalIF":1.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk factors for severe adverse drug reactions in hospitalized patients. 住院患者严重药物不良反应的危险因素。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1122

Nemanja Z Petrović, Ana V Pejčić, Miloš N Milosavljević, Slobodan M Janković

{"title":"Risk factors for severe adverse drug reactions in hospitalized patients.","authors":"Nemanja Z Petrović, Ana V Pejčić, Miloš N Milosavljević, Slobodan M Janković","doi":"10.1515/med-2024-1122","DOIUrl":"10.1515/med-2024-1122","url":null,"abstract":"Background: Severe adverse drug reactions (sADRs) are becoming increasingly common nowadays. The incidence of sADRs is approaching 6.7%, and the incidence of fatal adverse reactions is 0.32% in hospitalized patients. Of these, 48.5% are, at least potentially, preventable.Aims: This study's objective was to determine factors associated with the occurrence and preventability of sADRs occurring at the tertiary level.Methods: A case-control retrospective-prospective clinical observational study design was used for the study. The research cohort included patients hospitalized at the University Clinical Center (UCC) in Kragujevac, Serbia, from January 1, 2019 to January 1, 2024. The research comprised 147 individuals who were admitted to the UCC in Kragujevac. There were 49 patients with sADRs and 98 controls.Results: Significant factors associated with sADRs in our study were a total number of consultations (ORadjusted = 5.60), Charlson comorbidity index (ORadjusted = 0.30), C-reactive protein (ORadjusted = 1.07), prescribed antihistamines (ORadjusted = 14.37), and antihypertensives (ORadjusted = 0.15).Conclusion: We have identified the factors that are associated with sADRs should be kept in mind while working with patients at the tertiary level. Early detection of those factors may help with early notification of sADRs and their prevention.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241122"},"PeriodicalIF":1.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling novel biomarkers for platinum chemoresistance in ovarian cancer. 揭示卵巢癌铂化疗耐药的新生物标志物。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1084

Caixia Wang, Changsheng Peng, Chuan Xie

{"title":"Unveiling novel biomarkers for platinum chemoresistance in ovarian cancer.","authors":"Caixia Wang, Changsheng Peng, Chuan Xie","doi":"10.1515/med-2024-1084","DOIUrl":"10.1515/med-2024-1084","url":null,"abstract":"Primary chemoresistance to platinum-based treatment is observed in approximately 33% of individuals diagnosed with ovarian cancer; however, conventional clinical markers exhibit limited predictive value for chemoresistance. This study aimed to discover new genetic markers that can predict primary resistance to platinum-based chemotherapy. Through the analysis of three GEO datasets (GSE114206, GSE51373, and GSE63885) utilizing bioinformatics methodologies, we identified two specific genes, MFAP4 and EFEMP1. The findings revealed that the areas under the receiver operating characteristic curves for MFAP4 and EFEMP1 were 0.716 and 0.657 in the training cohort, and 0.629 and 0.746 in the testing cohort, respectively. In all cases or in cases treated with platin, high expression of MFAP4 and EFEMP1 was linked to shortened overall survival and progression-free survival. MFAP4 and EFEMP1 were positively correlated with epithelial-mesenchymal transition, TGF-β signaling, KRAS signaling, and so on. The high expression groups of MFAP4 and EFEMP1 exhibited elevated stromal, immune, and ESTIMATE scores. Finally, we constructed a regulatory network involving lncRNA-miRNA-mRNA interactions. In summary, MFAP4 and EFEMP1 have the potential to serve as predictive indicators for both response to platinum-based chemotherapy and survival rates, and might be regarded as innovative biomarkers and therapeutic targets for OC patients.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241084"},"PeriodicalIF":1.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing. 基于单细胞测序分析ALA-PDT对单核真菌感染小鼠足垫模型巨噬细胞的影响。

IF 1.7 4区医学

Open Medicine Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI: 10.1515/med-2024-1132

Wenyi Chen, Xuelin Wu, Muhammad Danish Yaqoob, Kangxing Liu, Yanqing Hu, Xiuling Ke, Yongxuan Hu

{"title":"Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing.","authors":"Wenyi Chen, Xuelin Wu, Muhammad Danish Yaqoob, Kangxing Liu, Yanqing Hu, Xiuling Ke, Yongxuan Hu","doi":"10.1515/med-2024-1132","DOIUrl":"10.1515/med-2024-1132","url":null,"abstract":"Chromoblastomycosis (CBM) is a chronic neglected fungal disease that causes serious damage to the physical and mental health of patients. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has garnered significant attention in the recent era for the treatment of CBM and has exhibited promising effects in several clinical case reports. We established a mice footpad infection model with Fonsecaea monophora and analyzed the impact of PDT treatment on the immune response of macrophages using single-cell sequencing. The results showed that infection of the mouse footpad skin with F. monophora results in an increase in inflammatory cells, primarily mononuclear-macrophages, with the activation of complement and enhancement of cell chemotaxis, leading to upregulation of anti-infection-related pathways. After ALA-PDT treatment, the number of inflammatory cells decreased, while macrophages upregulated the expression of antigen-recognition-related genes, enhancing phagocytosis and autophagy-related biological functions.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241132"},"PeriodicalIF":1.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0