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MARK1 suppress malignant progression of hepatocellular carcinoma and improves sorafenib resistance through negatively regulating POTEE. MARK1 通过负调控 POTEE 抑制肝细胞癌的恶性进展并改善索拉非尼的耐药性。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-11-07 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1060

Xin Lu, Zhiyuan Chen, Wenting Mi, Jianming Zheng, Yubin Liu

{"title":"MARK1 suppress malignant progression of hepatocellular carcinoma and improves sorafenib resistance through negatively regulating POTEE.","authors":"Xin Lu, Zhiyuan Chen, Wenting Mi, Jianming Zheng, Yubin Liu","doi":"10.1515/med-2024-1060","DOIUrl":"https://doi.org/10.1515/med-2024-1060","url":null,"abstract":"Purpose: This study aimed to investigate the role of microtubule-affinity regulatory protein kinase 1 (MARK1) in hepatocellular carcinoma (HCC) progression, its association with sorafenib sensitivity, and the interplay between MARK1 and POTE Ankyrin domain family member E(POTEE) in HCC cells.Methods: Quantitative real-time polymerase chain reaction analysis was used to assess MARK1 and POTEE expression in 60 pairs of HCC tissues and cell lines. The correlation between MARK1 levels, clinicopathological features, and patient prognosis was analyzed. Sorafenib-resistant HCC cell models were developed, followed by MARK1 overexpression to evaluate its impact on cell functions. Luciferase reporter assays and rescue experiments were conducted to elucidate the MARK1-POTEE regulatory mechanism.Results: MARK1 exhibited decreased mRNA expression in HCC tissues and cells, correlating with adverse clinicopathological features and poorer patient survival. Luciferase assays confirmed direct binding between MARK1 and POTEE. Sorafenib treatment increased MARK1 protein levels, reduced POTEE, and inhibited cell proliferation. Overexpressing MARK1 suppressed sorafenib-induced proliferation in resistant cells, while co-overexpression of MARK1 and POTEE reversed this effect.Conclusion: MARK1 potentially restrains HCC progression and enhances sorafenib resistance by negatively modulating POTEE expression, highlighting its significance as a therapeutic target in HCC treatment.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic role of respiratory exercise in patients with tuberculous pleurisy. 呼吸运动对结核性胸膜炎患者的治疗作用。

IF 1.7 4区医学

Open Medicine Pub Date : 2024-11-07 eCollection Date: 2024-01-01 DOI: 10.1515/med-2024-1057

ZengXia Ma, GuiMei Liu, GaoFeng Qiao, ChangMing Shen, Cheng Wang

{"title":"Therapeutic role of respiratory exercise in patients with tuberculous pleurisy.","authors":"ZengXia Ma, GuiMei Liu, GaoFeng Qiao, ChangMing Shen, Cheng Wang","doi":"10.1515/med-2024-1057","DOIUrl":"https://doi.org/10.1515/med-2024-1057","url":null,"abstract":"Objective: To observe the efficacy of respiratory exercise in patients with tuberculous pleurisy (TBP).Methods: A randomized controlled study was conducted including 146 patients diagnosed with TBP and undergoing pleural effusion drainage in Shandong Public Health Clinical Center from June 2020 to December 2022, and the patients were randomly divided into the control group and the respiratory exercise observation group. Pleural effusion drainage time, the difference of pulmonary function, and the degree of pleural hypertrophy between the two groups at 1 and 3 months after treatment were studied.Results: Compared with the control group, the pleural effusion drainage time of the observation group was shortened, and there was no significant difference between the two groups in terms of lung function and the degree of pleural hypertrophy at 1 month after treatment, while the lung function indexes and the degree of pleural hypertrophy of the observation group were significantly improved compared with that of the control group at 3 months after treatment.Conclusion: Respiratory exercise can shorten the drainage time of effusion in patients with TBP, and help to improve lung function and alleviate pleural hypertrophy adhesion.","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0