Open Biology最新文献_第8页

Simple, streamlined, cost-effective cDNA synthesis method from cell cultures. 简单，流线型，成本效益的cDNA合成方法从细胞培养。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1098/rsob.240226

Daniel Stránský, Monika Šteigerová, Markéta Kuklová, Veronika Hanzíková, Nikolina Canová, Jiří Novotný, Ladislav Šenolt, Ondřej Slanař

{"title":"Simple, streamlined, cost-effective cDNA synthesis method from cell cultures.","authors":"Daniel Stránský, Monika Šteigerová, Markéta Kuklová, Veronika Hanzíková, Nikolina Canová, Jiří Novotný, Ladislav Šenolt, Ondřej Slanař","doi":"10.1098/rsob.240226","DOIUrl":"10.1098/rsob.240226","url":null,"abstract":"Applications like drug development need simple and streamlined methods to process samples from 96-well cell culture plates for gene expression measurements. Unfortunately, current options are expensive for such processing. Therefore, our aim was to develop a method that would allow streamlined analysis of mRNA from 96-well cell culture plates while being relatively cheap and simple. We developed a method based on the qPCR 'Cells-to-cDNA' approach and validated it against commercially available kits using the same approach or spin columns-based RNA purification. For this purpose, we conducted a series of comparisons of gene expression from peripheral blood mononuclear cells, SK-HEP-1 and U-87 cell cultures in 96-well plates. Our final method involved lysing cells with 25-100 µl solution of 0.5% SDS, 10 mM DTT, 1 mg ml-1 proteinase K dissolved in water, 1 h incubation at 50°C, followed by proteinase K inactivation at 90°C for 5 min and lysate neutralization with 1 : 1 dilution by 20% Tween 20 solution. Reverse transcription and qPCR were carried out using standard methods. This method showed a mean reduction of Ct ± s.d. value by 2.4 ± 1.3 compared with the 'Cells-to-cDNA' kit and by 1.4 ± 0.5 compared with the RNA purification kit with lower variability.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240226"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pigment-dispersing factor neuropeptides act as multifunctional hormones and modulators in tardigrades. 色素分散因子神经肽是缓步动物的多功能激素和调节剂。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-05 DOI: 10.1098/rsob.240242

Soumi Dutta, Lars Hering, Milena M Grollmann, Niklas Metzendorf, Vladimir Gross, Kazuharu Arakawa, Susanne Neupert, Monika Stengl, Friedrich W Herberg, Georg Mayer

{"title":"Pigment-dispersing factor neuropeptides act as multifunctional hormones and modulators in tardigrades.","authors":"Soumi Dutta, Lars Hering, Milena M Grollmann, Niklas Metzendorf, Vladimir Gross, Kazuharu Arakawa, Susanne Neupert, Monika Stengl, Friedrich W Herberg, Georg Mayer","doi":"10.1098/rsob.240242","DOIUrl":"10.1098/rsob.240242","url":null,"abstract":"Pigment-dispersing factors (PDFs) are neuropeptides that play key roles in controlling the circadian rhythms in various insects, whereas their function remains elusive in other protostomes including tardigrades (water bears). Here we show that the three PDFs of the tardigrade Hypsibius exemplaris are co-localized in two pairs of inner lobe cells in the brain, whereas only one PDF occurs in four additional cerebral and two extracerebral cells. The axons of the inner lobe cells pass through the contralateral brain hemisphere, descend to the ventral nerve cord and terminate in two pairs of potential release sites in the posteriormost trunk ganglion. Using in vitro assays, we demonstrate that all three PDFs and their deorphanized receptor (PDFR) are functional. Widespread localization of PDFR suggests that tardigrade PDFs may act as multifunctional hormones and neuromodulators that control major functions including light detection, neural processing, locomotion, feeding, digestion, osmoregulation, growth, embryonic development and oogenesis/reproduction.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240242"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic regulation and variation of fetal plasma metabolome in the context of sex, paternal breeds and variable fetal weight. 性别、父系品种和胎儿体重变化背景下胎儿血浆代谢组的遗传调控和变异。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-05 DOI: 10.1098/rsob.240285

Siriluck Ponsuksili, Eduard Murani, Beate Fuchs, Christina E Galuska, Henry Reyer, Muhammad Arsalan Iqbal, Shuaichen Li, Michael Oster, Klaus Wimmers

{"title":"Genetic regulation and variation of fetal plasma metabolome in the context of sex, paternal breeds and variable fetal weight.","authors":"Siriluck Ponsuksili, Eduard Murani, Beate Fuchs, Christina E Galuska, Henry Reyer, Muhammad Arsalan Iqbal, Shuaichen Li, Michael Oster, Klaus Wimmers","doi":"10.1098/rsob.240285","DOIUrl":"10.1098/rsob.240285","url":null,"abstract":"Metabolic processes in fetuses can significantly influence piglet weight at birth. Understanding the genetic determinants of systemic metabolism is crucial for uncovering how genetic and molecular pathways impact biological mechanisms, particularly during the fetal phase. We present data on 1112 plasma metabolites using untargeted ultra-high performance liquid chromatography-tandem mass spectrometry methods, of 260 backcross (BC) fetuses from two sires' breeds at 63 days post-conception. Eight chemical superclasses have been identified, with lipids accounting for the majority of metabolites. Genomic heritability (h²) was estimated for each metabolite, revealing that 50% had h² values below 0.2, with a higher average in the amino acid class compared with the lipid. We annotated 448 significant metabolite quantitative trait loci associated with 10 metabolites, primarily lipids, indicating strong genetic regulation. Additionally, metabolite associations with sex, fetal weight and sire's breed were explored, revealing significant associations for 354 metabolites. Fetal weight influenced the largest number of metabolites, particularly glycerophospholipids and sphingolipids, emphasizing the genetic and metabolic complexity underlying fetal development. These findings enhance our understanding of the genetic regulation of metabolite levels and their associations with key phenotypic traits in fetuses, providing insights into metabolic pathways, potential biomarkers and serving as a baseline dataset for metabolomics studies of fetuses.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240285"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limitations of sequence dissimilarity as a predictor of prokaryotic lineage. 序列异质性作为原核生物血统预测指标的局限性

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-19 DOI: 10.1098/rsob.240302

Alvar A Lavin, Juan Rivas-Santisteban

引用次数: 0

Schwann cells in regeneration and cancer: an epithelial-mesenchymal transition perspective. 雪旺细胞再生和癌症：上皮-间质转变的观点。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-05 DOI: 10.1098/rsob.240337

Francisco Gracia, Berta Sanchez-Laorden, Jose A Gomez-Sanchez

{"title":"Schwann cells in regeneration and cancer: an epithelial-mesenchymal transition perspective.","authors":"Francisco Gracia, Berta Sanchez-Laorden, Jose A Gomez-Sanchez","doi":"10.1098/rsob.240337","DOIUrl":"10.1098/rsob.240337","url":null,"abstract":"In the peripheral nervous system, glial cells, known as Schwann cells (SCs), are responsible for supporting and maintaining nerves. One of the most important characteristics of SCs is their remarkable plasticity. In various injury contexts, SCs undergo a reprogramming process that generates specialized cells to promote tissue regeneration and repair. However, in pathological conditions, this same plasticity and regenerative potential can be hijacked. Different studies highlight the activation of the epithelial-mesenchymal transition (EMT) as a driver of SC phenotypic plasticity. Although SCs are not epithelial, their neural crest origin makes EMT activation crucial for their ability to adopt repair phenotypes, mirroring the plasticity observed during development. These adaptive processes are essential for regeneration. However, EMT activation in SCs-derived tumours enhances cancer progression and aggressiveness. Furthermore, in the tumour microenvironment (TME), SCs also acquire activated phenotypes that contribute to tumour migration and invasion by activating EMT in cancer cells. In this review, we will discuss how EMT impacts SC plasticity and function from development and tissue regeneration to pathological conditions, such as cancer.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240337"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between circadian and photoperiodic latitudinal clines in Drosophila littoralis. 沿海果蝇昼夜与光周期纬度线的相关性。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-05 DOI: 10.1098/rsob.240403

Giulia Manoli, Pekka Lankinen, Enrico Bertolini, Charlotte Helfrich-Förster

{"title":"Correlation between circadian and photoperiodic latitudinal clines in Drosophila littoralis.","authors":"Giulia Manoli, Pekka Lankinen, Enrico Bertolini, Charlotte Helfrich-Förster","doi":"10.1098/rsob.240403","DOIUrl":"10.1098/rsob.240403","url":null,"abstract":"Insects can survive harsh conditions, including Arctic winters, by entering a hormonally induced state of dormancy, known as diapause. Diapause is triggered by environmental cues such as shortening of the photoperiod (lengthening of the night). The time of entry into diapause depends on the latitude of the insects' habitat, and this applies even within a species: populations living at higher latitudes enter diapause earlier in the year than populations living at lower latitudes. A long-standing question in biology is whether the internal circadian clock, which governs daily behaviour and serves as a reference clock to measure night length, shows similar latitudinal adaptations. To address this question, we examined the onset of diapause and various behavioural and molecular parameters of the circadian clock in the cosmopolitan fly, Drosophila littoralis, a species distributed throughout Europe from the Black Sea (41° N) to Arctic regions (69° N). We found that all clock parameters examined showed the same correlation with latitude as the critical night length for diapause induction. We conclude that the circadian clock has adapted to the latitude and that this may result in the observed latitudinal differences in the onset of diapause.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240403"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering respiratory viral infections by harnessing organ-on-chip technology to explore the gut-lung axis. 利用器官芯片技术探索肠-肺轴，破解呼吸道病毒感染。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-05 DOI: 10.1098/rsob.240231

Hristina Koceva, Mona Amiratashani, Parastoo Akbarimoghaddam, Bianca Hoffmann, Gaukhar Zhurgenbayeva, Mark S Gresnigt, Vanessa Rossetto Marcelino, Christian Eggeling, Marc Thilo Figge, Maria-João Amorim, Alexander S Mosig

{"title":"Deciphering respiratory viral infections by harnessing organ-on-chip technology to explore the gut-lung axis.","authors":"Hristina Koceva, Mona Amiratashani, Parastoo Akbarimoghaddam, Bianca Hoffmann, Gaukhar Zhurgenbayeva, Mark S Gresnigt, Vanessa Rossetto Marcelino, Christian Eggeling, Marc Thilo Figge, Maria-João Amorim, Alexander S Mosig","doi":"10.1098/rsob.240231","DOIUrl":"10.1098/rsob.240231","url":null,"abstract":"The lung microbiome has recently gained attention for potentially affecting respiratory viral infections, including influenza A virus, respiratory syncytial virus (RSV) and SARS-CoV-2. We will discuss the complexities of the lung microenvironment in the context of viral infections and the use of organ-on-chip (OoC) models in replicating the respiratory tract milieu to aid in understanding the role of temporary microbial colonization. Leveraging the innovative capabilities of OoC, particularly through integrating gut and lung models, opens new avenues to understand the mechanisms linking inter-organ crosstalk and respiratory infections. We will discuss technical aspects of OoC lung models, ranging from the selection of cell substrates for extracellular matrix mimicry, mechanical strain, breathing mechanisms and air-liquid interface to the integration of immune cells and use of microscopy tools for algorithm-based image analysis and systems biology to study viral infection in vitro. OoC offers exciting new options to study viral infections across host species and to investigate human cellular physiology at a personalized level. This review bridges the gap between complex biological phenomena and the technical prowess of OoC models, providing a comprehensive roadmap for researchers in the field.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240231"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleolar origins: challenging perspectives on evolution and function. 核仁起源：进化和功能的挑战观点。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1098/rsob.240330

Israel Muñoz-Velasco, Ana Karen Herrera-Escamilla, Alberto Vázquez-Salazar

{"title":"Nucleolar origins: challenging perspectives on evolution and function.","authors":"Israel Muñoz-Velasco, Ana Karen Herrera-Escamilla, Alberto Vázquez-Salazar","doi":"10.1098/rsob.240330","DOIUrl":"10.1098/rsob.240330","url":null,"abstract":"The nucleolus, once considered a mere 'ribosome factory', is now recognized as a dynamic hub influencing nearly every aspect of cellular life, from genome organization to stress response and ageing. Despite being a hallmark of eukaryotic cells, recent discoveries reveal that even prokaryotes exhibit nucleolus-like structures, hinting at ancient origins for nucleolar functions. This review explores the evolutionary journey of the nucleolus, tracing its roots back to early life and examining its structural and functional diversity across domains. We highlight key nucleolar proteins that play vital roles not only in ribosome production but also in regulating cell cycle, DNA repair and cellular stress, linking nucleolar activity directly to health and disease. Dysfunctions in nucleolar processes are implicated in cancer, ribosomopathies and neurodegenerative disorders, positioning the nucleolus as a critical target for innovative therapeutic strategies. As advanced imaging and molecular techniques unlock deeper insights into both canonical and mysterious non-canonical roles, the nucleolus stands as a model for how cellular microenvironments can evolve to meet complex biological demands. By addressing open questions surrounding the evolution of the nucleolus, its organization and diverse functions, the ideas presented here aim to contribute to the ongoing discussion, challenging traditional paradigms and suggesting new avenues for uncovering the fundamental principles that drive cellular life.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240330"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The proteotranscriptomic characterization of venom in the white seafan Eunicella singularis elucidates the evolution of Octocorallia arsenal. 白色海洋单根unicella singularis毒液的蛋白质转录组学特征阐明了Octocorallia库的进化。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1098/rsob.250015

Maria Vittoria Modica, Serena Leone, Marco Gerdol, Samuele Greco, Didier Aurelle, Marco Oliverio, Giulia Fassio, Khadija El Koulali, Célia Barrachina, Sebastien Dutertre

{"title":"The proteotranscriptomic characterization of venom in the white seafan Eunicella singularis elucidates the evolution of Octocorallia arsenal.","authors":"Maria Vittoria Modica, Serena Leone, Marco Gerdol, Samuele Greco, Didier Aurelle, Marco Oliverio, Giulia Fassio, Khadija El Koulali, Célia Barrachina, Sebastien Dutertre","doi":"10.1098/rsob.250015","DOIUrl":"10.1098/rsob.250015","url":null,"abstract":"All the members of the phylum Cnidaria are characterized by the production of venom in specialized structures, the nematocysts. Venom of jellyfish (Medusozoa) and sea anemones (Anthozoa) has been investigated since the 1970s, revealing a remarkable molecular diversity. Specifically, sea anemones harbour a rich repertoire of neurotoxic peptides, some of which have been developed in drug leads. However, venoms of the vast majority of Anthozoa species remain uncharacterized, particularly in the class Octocorallia. To fill this gap, we applied a proteo-transcriptomic approach to investigate venom composition in Eunicella singularis, a gorgonian species common in Mediterranean hard-bottom benthic communities. Our results highlighted the peculiarities of the venom of E. singularis with respect to sea anemones, which is reflected in the presence of several toxins with novel folds, worthy of functional characterization. A comparative genomic survey across the octocoral radiation allowed us to generalize these findings and provided insights into the evolutionary history, molecular diversification patterns and putative adaptive roles of venom toxins. A comparison of whole-body and nematocyst proteomes revealed the presence of different cytolytic toxins inside and outside the nematocysts. Two instances of differential maturation patterns of toxin precursors were also identified, highlighting the intricate regulatory pathways underlying toxin expression.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"250015"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of resistance and disease tolerance mechanisms to oral bacterial infection in Drosophila melanogaster. 黑腹果蝇对口腔细菌感染的耐药性和疾病耐受机制的进化。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1098/rsob.240265

Tânia F Paulo, Priscilla A Akyaw, Tiago Paixão, Élio Sucena

{"title":"Evolution of resistance and disease tolerance mechanisms to oral bacterial infection in Drosophila melanogaster.","authors":"Tânia F Paulo, Priscilla A Akyaw, Tiago Paixão, Élio Sucena","doi":"10.1098/rsob.240265","DOIUrl":"10.1098/rsob.240265","url":null,"abstract":"Pathogens exert strong selection on hosts that evolve and deploy different defensive strategies, namely minimizing pathogen exposure (avoidance), directly promoting pathogen elimination (resistance) and/or managing the deleterious effects of illness (disease tolerance). However, how the host response partitions across these processes has not been directly tested in a single host-pathogen system, let alone in the context of known adaptive trajectories resulting from experimental evolution. Here, we compare a Drosophila melanogaster population adapted to oral infection with its natural pathogen Pseudomonas entomophila (BactOral), to its control population to find no evidence for behavioural changes but measurable differences in both resistance and disease tolerance. In BactOral, no differences were detected in bacterial intake or defecation, nor gut cell renewal. However, a measurable relative decrease in bacterial loads correlates with an increase in gut-specific anti-microbial peptide production, pointing to a strengthening in resistance. Additionally, we posit that disease tolerance also contributes to the response of BactOral through a tighter control of self- and pathogen-derived damage caused by bacteria exposure. This study reveals a genetically complex and mechanistically multi-layered response, possibly reflecting the structure of adaptation to infection in natural populations.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240265"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0