Open Biology最新文献_第7页

Integrated multi-omics identify key signalling pathways for notochord lumenogenesis in ascidian Ciona savignyi. 综合多组学鉴定海鞘脊索放光发生的关键信号通路。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-04-01 Epub Date: 2025-04-09 DOI: 10.1098/rsob.240402

Jin Zhang, Zicheng Tan, Qishu Qin, Hongzhe Peng, Wenjie Shi, Haiyan Yu, Bo Dong

{"title":"Integrated multi-omics identify key signalling pathways for notochord lumenogenesis in ascidian Ciona savignyi.","authors":"Jin Zhang, Zicheng Tan, Qishu Qin, Hongzhe Peng, Wenjie Shi, Haiyan Yu, Bo Dong","doi":"10.1098/rsob.240402","DOIUrl":"10.1098/rsob.240402","url":null,"abstract":"Lumen formation and inflation are crucial for tubular organ morphogenesis and function. However, the key signalling pathways for lumenogenesis regulation were not well identified. Here, we performed tissue-specific transcriptomic sequencing for the isolated Ciona notochord tissue, in which 10 551 genes in total were identified. To investigate crucial signalling pathways in regulating lumenogenesis, KEGG was performed and the results showed that the Rap1 signalling pathway, vascular endothelial growth factor signalling pathway, mitogen activated protein kinase (MAPK) signalling pathway (plant) and Ras signalling pathway might play important roles in lumenogenesis. Moreover, correlation analysis with proteomic data and comparison analysis of single-cell transcriptomic data were further utilized to identify the potential critical roles of the Rap1 signalling pathway and Ras signalling pathway in lumenogenesis. To verify their functions in lumenogenesis, the Ras/calcium-Rap1-MAPK signalling axis was blocked, and the results showed that the notochord lumenogenesis failed. Meanwhile, we identified that CDC42 was a potential downstream target factor of the Ras-Rap1-MAPK signalling axis, playing crucial functions in notochord lumenogenesis. Overall, we systematically revealed the key regulatory signalling pathways for notochord lumen formation and verified a lumenogenesis-related signalling axis, providing a foundational data resource for exploring the mechanisms of lumenogenesis.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 4","pages":"240402"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the bioluminescence systems of three sea pens (Cnidaria: Anthozoa): from de novo transcriptome analyses to biochemical assays. 对三种海笔（刺胞纲：珊瑚虫纲）生物发光系统的洞察：从从头转录组分析到生化分析。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-04-01 Epub Date: 2025-04-30 DOI: 10.1098/rsob.240262

Laurent Duchatelet, Gabriela A Galeazzo, Constance Coubris, Laure Bridoux, René Rezsohazy, Marcelo R S Melo, Milan Marek, Danilo T Amaral, Sam Dupont, Anderson Garbuglio de Oliveira, Jérôme Delroisse

{"title":"Insights into the bioluminescence systems of three sea pens (Cnidaria: Anthozoa): from de novo transcriptome analyses to biochemical assays.","authors":"Laurent Duchatelet, Gabriela A Galeazzo, Constance Coubris, Laure Bridoux, René Rezsohazy, Marcelo R S Melo, Milan Marek, Danilo T Amaral, Sam Dupont, Anderson Garbuglio de Oliveira, Jérôme Delroisse","doi":"10.1098/rsob.240262","DOIUrl":"https://doi.org/10.1098/rsob.240262","url":null,"abstract":"Bioluminescence is the production of visible light by living organisms. It occurs through the oxidation of luciferin substrates catalysed by luciferase enzymes. Auxiliary proteins, such as fluorescent proteins and luciferin-binding proteins, can modify the light emitted wavelength or stabilize reactive luciferin molecules, respectively. Additionally, calcium ions are crucial for the luminescence across various species. Despite the large phylogenetic distribution of bioluminescent organisms, only a few systems have been comprehensively studied. Notably, cnidarian species of the Renilla genus utilize a coelenterazine-dependent luciferase, a calcium-dependent coelenterazine-binding protein and a green fluorescent protein. We investigated the bioluminescence of three sea pen species: Pennatula phosphorea, Anthoptilum murrayi and Funiculina quadrangularis (Pennatuloidea, Anthozoa). Their light-emission spectra reveal peaks at 510, 513 and 485 nm, respectively. A coelenterazine-based reaction was demonstrated in all three species. Using transcriptome analyses, we identified transcripts coding for luciferases, green fluorescent proteins and coelenterazine-binding proteins for P. phosphorea and A. murrayi. Immunodetection confirmed the expression of luciferase in P. phosphorea and F. quadrangularis. We also expressed recombinant luciferase of A. murrayi, confirming its activity. We highlighted the role of calcium ions in bioluminescence, possibly associated with the mechanism of substrate release at the level of coelenterazine-binding proteins. The study proposes a model for anthozoan bioluminescence, offering new avenues for future ecological and functional research on these luminous organisms.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 4","pages":"240262"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota diversity affects fish behaviour and is influenced by host genetics and early rearing conditions. 肠道菌群多样性影响鱼类行为，并受宿主遗传和早期饲养条件的影响。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-04-01 Epub Date: 2025-04-16 DOI: 10.1098/rsob.240232

Ishrat Z Anka, Tamsyn Uren Webster, Sam McLaughlin, Benjamin Overland, Matthew Hitchings, Carlos Garcia de Leaniz, Sofia Consuegra

{"title":"Gut microbiota diversity affects fish behaviour and is influenced by host genetics and early rearing conditions.","authors":"Ishrat Z Anka, Tamsyn Uren Webster, Sam McLaughlin, Benjamin Overland, Matthew Hitchings, Carlos Garcia de Leaniz, Sofia Consuegra","doi":"10.1098/rsob.240232","DOIUrl":"https://doi.org/10.1098/rsob.240232","url":null,"abstract":"The gut microbiota influences human and animal cognition and behaviour through its effects on the endocrine and immune systems. The microbiome-behaviour relationship may be especially relevant for fish, due to their diverse evolutionary history and potential implications for farming and conservation. Yet, there is limited research on the interaction between gut microbiome and behaviour in non-model fish. We manipulated the rearing environment and diet of fish from two inbred strains of the self-fertilizing mangrove killifish (Kryptolebias marmoratus) and assessed the effects on the gut microbiome and its interactions with anxiety-like behaviours. We found that microbiota composition and alpha diversity were significantly influenced by host genetics (strain), hatching mode (naturally or artificial dechorionation) and diet, but not by environmental enrichment. Fish activity level and inspections of a novel object were strongly associated with microbiota community composition and alpha diversity. The microbial taxa associated with differences in behaviour were dominated by Bacteroidales, potentially related to the production of metabolites affecting neural development. We suggest that the association between microbiome and fish behaviour could be an indirect effect of the modulation of the gut microbiota by host genetics and early rearing conditions, which could be affecting the production of microbial metabolites that interact with the fish physiology.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 4","pages":"240232"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cell origin of reactive oxygen species and its implication for evolutionary trade-offs. 活性氧的细胞起源及其对进化权衡的影响。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-04-01 Epub Date: 2025-04-16 DOI: 10.1098/rsob.240312

Maïly Kervella, Fabrice Bertile, Frédéric Bouillaud, François Criscuolo

{"title":"The cell origin of reactive oxygen species and its implication for evolutionary trade-offs.","authors":"Maïly Kervella, Fabrice Bertile, Frédéric Bouillaud, François Criscuolo","doi":"10.1098/rsob.240312","DOIUrl":"https://doi.org/10.1098/rsob.240312","url":null,"abstract":"The allocation of resources in animals is shaped by adaptive trade-offs aimed at maximizing fitness. At the heart of these trade-offs, lies metabolism and the conversion of food resources into energy, a process mostly occurring in mitochondria. Yet, the conversion of nutrients to utilizable energy molecules (adenosine triphosphate) inevitably leads to the by-production of reactive oxygen species (ROS) that may cause damage to important biomolecules such as proteins or lipids. The 'ROS theory of ageing' has thus proposed that the relationship between lifespan and metabolic rate may be mediated by ROS production. However, the relationship is not as straightforward as it may seem: not only are mitochondrial ROS crucial for various cellular functions, but mitochondria are also actually equipped with antioxidant systems, and many extra-mitochondrial sources also produce ROS. In this review, we discuss how viewing the mitochondrion as a regulator of cellular oxidative homeostasis, not merely a ROS producer, may provide new insights into the role of oxidative stress in the reproduction-survival trade-off. We suggest several avenues to test how mitochondrial oxidative buffering capacity might complement current bioenergetic and evolutionary studies.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 4","pages":"240312"},"PeriodicalIF":4.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Footprints in the Sno: investigating the cellular and molecular mechanisms of SNORD116. snord的细胞和分子机制研究[j]。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-19 DOI: 10.1098/rsob.240371

Terri L Holmes, Alzbeta Chabronova, Chris Denning, Victoria James, Mandy J Peffers, James G W Smith

引用次数: 0

C-terminal glycosylation of type IX secretion system cargo proteins in Prevotella intermedia with both short and long secretion signals. 普雷沃氏菌分泌系统中具有短、长分泌信号的转运蛋白的c端糖基化。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-26 DOI: 10.1098/rsob.240335

Xi Ye, Nabil Bin Rustam, Dhana Gorasia, Eric Reynolds, Debnath Ghosal, Paul Veith

{"title":"C-terminal glycosylation of type IX secretion system cargo proteins in Prevotella intermedia with both short and long secretion signals.","authors":"Xi Ye, Nabil Bin Rustam, Dhana Gorasia, Eric Reynolds, Debnath Ghosal, Paul Veith","doi":"10.1098/rsob.240335","DOIUrl":"10.1098/rsob.240335","url":null,"abstract":"Prevotella intermedia is a Gram-negative bacterium that is associated with periodontitis and acute necrotizing ulcerative gingivitis. P. intermedia utilizes the type IX secretion system (T9SS) to secrete and anchor virulence factors to the cell surface, presumably via C-terminal glycosylation. The identity of the linking sugar and the sites of modification on the cargo are unknown. Here, we employed hidden Markov models to predict cargo proteins in P. intermedia and conducted LC-MS/MS analyses of partially deglycosylated fractions to characterize the C-terminal glycosylation. A total of 80 cargo proteins were predicted based on the presence of a T9SS C-terminal domain (CTD) signal, and these were divided into 48 short CTDs and 32 long CTDs. Cleavage sites for five short and four long CTDs were experimentally determined, and glycosylation was observed at the mature C-terminus of six cargo. Two glycans were identified of delta masses 419.198 and 433.185 Da, corresponding to novel C-terminal amide linkages to N-alanyl dHex-HexNAc and N-alanyl (Me-dHex)-HexNAc, respectively. This indicated that both short and long CTDs supported cleavage and glycosylation. AlphaFold multimer modelling predicted that both kinds of CTDs could bind to the PorV shuttle protein in the same manner, with the conserved CTD motifs interacting with the same sites in PorV.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240335"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycomb-mediated transgenerational epigenetic inheritance of Drosophila eye colour is independent of small RNAs. 果蝇眼睛颜色的多梳介导的跨代表观遗传与小rna无关。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1098/rsob.240298

Maximilian Fitz-James, Poppy Sparrow, Christopher Paton, Peter Sarkies

{"title":"Polycomb-mediated transgenerational epigenetic inheritance of Drosophila eye colour is independent of small RNAs.","authors":"Maximilian Fitz-James, Poppy Sparrow, Christopher Paton, Peter Sarkies","doi":"10.1098/rsob.240298","DOIUrl":"10.1098/rsob.240298","url":null,"abstract":"Transgenerational epigenetic inheritance (TEI) describes the process whereby distinct epigenetic states are transmitted between generations, resulting in heritable gene expression and phenotypic differences that are independent of DNA sequence variation. Chromatin modifications have been demonstrated to be important in TEI; however, the extent to which they require other signals to establish and maintain epigenetic states is still unclear. Here we investigate whether small non-coding RNAs contribute to different epigenetic states of the Fab2L transgene in Drosophila triggered by transient long-range chromosomal contacts, which requires Polycomb complex activity to deposit the H3K27me3 modification for long-term TEI. By analysing mutants deficient in small non-coding RNAs, high-throughput sequencing data, long-range chromosomal contacts and gene expression, we demonstrate that small non-coding RNAs do not contribute directly to initiation or maintenance of silencing. However, we uncover an indirect role for microRNA expression in transgene silencing through effects on the Polycomb group gene Pleiohomeotic. Additionally, we show that a commonly used marker gene, Stubble (Sb), affects Pleiohomeotic expression, which may be important in interpreting experiments assaying Polycomb function in Drosophila development. By ruling out a plausible candidate for TEI at the Fab2L transgene, our work highlights the variability in different modes of TEI across species.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240298"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple, streamlined, cost-effective cDNA synthesis method from cell cultures. 简单，流线型，成本效益的cDNA合成方法从细胞培养。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1098/rsob.240226

Daniel Stránský, Monika Šteigerová, Markéta Kuklová, Veronika Hanzíková, Nikolina Canová, Jiří Novotný, Ladislav Šenolt, Ondřej Slanař

{"title":"Simple, streamlined, cost-effective cDNA synthesis method from cell cultures.","authors":"Daniel Stránský, Monika Šteigerová, Markéta Kuklová, Veronika Hanzíková, Nikolina Canová, Jiří Novotný, Ladislav Šenolt, Ondřej Slanař","doi":"10.1098/rsob.240226","DOIUrl":"10.1098/rsob.240226","url":null,"abstract":"Applications like drug development need simple and streamlined methods to process samples from 96-well cell culture plates for gene expression measurements. Unfortunately, current options are expensive for such processing. Therefore, our aim was to develop a method that would allow streamlined analysis of mRNA from 96-well cell culture plates while being relatively cheap and simple. We developed a method based on the qPCR 'Cells-to-cDNA' approach and validated it against commercially available kits using the same approach or spin columns-based RNA purification. For this purpose, we conducted a series of comparisons of gene expression from peripheral blood mononuclear cells, SK-HEP-1 and U-87 cell cultures in 96-well plates. Our final method involved lysing cells with 25-100 µl solution of 0.5% SDS, 10 mM DTT, 1 mg ml-1 proteinase K dissolved in water, 1 h incubation at 50°C, followed by proteinase K inactivation at 90°C for 5 min and lysate neutralization with 1 : 1 dilution by 20% Tween 20 solution. Reverse transcription and qPCR were carried out using standard methods. This method showed a mean reduction of Ct ± s.d. value by 2.4 ± 1.3 compared with the 'Cells-to-cDNA' kit and by 1.4 ± 0.5 compared with the RNA purification kit with lower variability.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240226"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pigment-dispersing factor neuropeptides act as multifunctional hormones and modulators in tardigrades. 色素分散因子神经肽是缓步动物的多功能激素和调节剂。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-05 DOI: 10.1098/rsob.240242

Soumi Dutta, Lars Hering, Milena M Grollmann, Niklas Metzendorf, Vladimir Gross, Kazuharu Arakawa, Susanne Neupert, Monika Stengl, Friedrich W Herberg, Georg Mayer

{"title":"Pigment-dispersing factor neuropeptides act as multifunctional hormones and modulators in tardigrades.","authors":"Soumi Dutta, Lars Hering, Milena M Grollmann, Niklas Metzendorf, Vladimir Gross, Kazuharu Arakawa, Susanne Neupert, Monika Stengl, Friedrich W Herberg, Georg Mayer","doi":"10.1098/rsob.240242","DOIUrl":"10.1098/rsob.240242","url":null,"abstract":"Pigment-dispersing factors (PDFs) are neuropeptides that play key roles in controlling the circadian rhythms in various insects, whereas their function remains elusive in other protostomes including tardigrades (water bears). Here we show that the three PDFs of the tardigrade Hypsibius exemplaris are co-localized in two pairs of inner lobe cells in the brain, whereas only one PDF occurs in four additional cerebral and two extracerebral cells. The axons of the inner lobe cells pass through the contralateral brain hemisphere, descend to the ventral nerve cord and terminate in two pairs of potential release sites in the posteriormost trunk ganglion. Using in vitro assays, we demonstrate that all three PDFs and their deorphanized receptor (PDFR) are functional. Widespread localization of PDFR suggests that tardigrade PDFs may act as multifunctional hormones and neuromodulators that control major functions including light detection, neural processing, locomotion, feeding, digestion, osmoregulation, growth, embryonic development and oogenesis/reproduction.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240242"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic regulation and variation of fetal plasma metabolome in the context of sex, paternal breeds and variable fetal weight. 性别、父系品种和胎儿体重变化背景下胎儿血浆代谢组的遗传调控和变异。

IF 4.5 3区生物学

Open Biology Pub Date : 2025-03-01 Epub Date: 2025-03-05 DOI: 10.1098/rsob.240285

Siriluck Ponsuksili, Eduard Murani, Beate Fuchs, Christina E Galuska, Henry Reyer, Muhammad Arsalan Iqbal, Shuaichen Li, Michael Oster, Klaus Wimmers

{"title":"Genetic regulation and variation of fetal plasma metabolome in the context of sex, paternal breeds and variable fetal weight.","authors":"Siriluck Ponsuksili, Eduard Murani, Beate Fuchs, Christina E Galuska, Henry Reyer, Muhammad Arsalan Iqbal, Shuaichen Li, Michael Oster, Klaus Wimmers","doi":"10.1098/rsob.240285","DOIUrl":"10.1098/rsob.240285","url":null,"abstract":"Metabolic processes in fetuses can significantly influence piglet weight at birth. Understanding the genetic determinants of systemic metabolism is crucial for uncovering how genetic and molecular pathways impact biological mechanisms, particularly during the fetal phase. We present data on 1112 plasma metabolites using untargeted ultra-high performance liquid chromatography-tandem mass spectrometry methods, of 260 backcross (BC) fetuses from two sires' breeds at 63 days post-conception. Eight chemical superclasses have been identified, with lipids accounting for the majority of metabolites. Genomic heritability (h²) was estimated for each metabolite, revealing that 50% had h² values below 0.2, with a higher average in the amino acid class compared with the lipid. We annotated 448 significant metabolite quantitative trait loci associated with 10 metabolites, primarily lipids, indicating strong genetic regulation. Additionally, metabolite associations with sex, fetal weight and sire's breed were explored, revealing significant associations for 354 metabolites. Fetal weight influenced the largest number of metabolites, particularly glycerophospholipids and sphingolipids, emphasizing the genetic and metabolic complexity underlying fetal development. These findings enhance our understanding of the genetic regulation of metabolite levels and their associations with key phenotypic traits in fetuses, providing insights into metabolic pathways, potential biomarkers and serving as a baseline dataset for metabolomics studies of fetuses.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"15 3","pages":"240285"},"PeriodicalIF":4.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0