Open Biology最新文献_第9页

Comment on: 'The proximal centriole age in spermatozoa is a potential reason for its different fate in the zygote after fertilization' Uzbekov and Avidor-Reiss 2024. 评论精子近端中心粒的年龄是其受精后在合子中不同命运的潜在原因》，Uzbekov 和 Avidor-Reiss 2024。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-03-01 Epub Date: 2024-03-06 DOI: 10.1098/rsob.230458

Rustem Uzbekov, Tomer Avidor-Reiss

引用次数: 0

The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle. 裂殖酵母 NDR 激酶 Orb6 及其信号通路 MOR 在细胞周期中调控细胞质微管组织。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-03-01 Epub Date: 2024-03-06 DOI: 10.1098/rsob.230440

Kazunori Kume, Kenji Nishikawa, Rikuto Furuyama, Takahiro Fujimoto, Takayuki Koyano, Makoto Matsuyama, Masaki Mizunuma, Dai Hirata

{"title":"The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle.","authors":"Kazunori Kume, Kenji Nishikawa, Rikuto Furuyama, Takahiro Fujimoto, Takayuki Koyano, Makoto Matsuyama, Masaki Mizunuma, Dai Hirata","doi":"10.1098/rsob.230440","DOIUrl":"10.1098/rsob.230440","url":null,"abstract":"Microtubule organization and reorganization during the cell cycle are achieved by regulation of the number, distribution and activity of microtubule-organizing centres (MTOCs). In fission yeast, the Mto1/2 complex determines the activity and distribution of cytoplasmic MTOCs. Upon mitosis, cytoplasmic microtubule nucleation ceases; inactivation of the Mto1/2 complex is triggered by Mto2 hyperphosphorylation. However, the protein kinase(s) that phosphorylates Mto2 remains elusive. Here we show that a conserved signalling network, called MOR (morphogenesis Orb6 network) in fission yeast, negatively regulates cytoplasmic MTOCs through Mto2 phosphorylation to ensure proper microtubule organization. Inactivation of Orb6 kinase, the most downstream MOR component, by attenuation of MOR signalling leads to reduced Mto2 phosphorylation, coincident with increased number of both Mto2 puncta and cytoplasmic microtubules. These defects cause the emergence of uncoordinated mitotic cells with cytoplasmic microtubules, resulting in reduced spindle assembly. Thus, the regulation of Mto2 by the MOR is crucial for cytoplasmic microtubule organization and contributes to reorganization of the microtubule cytoskeletons during the cell cycle.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 3","pages":"230440"},"PeriodicalIF":5.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140040091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The subapical labial sensory organ of spotted lanternfly Lycorma delicatula. 斑灯蝇 Lycorma delicatula 的唇下感觉器官。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-03-01 Epub Date: 2024-03-27 DOI: 10.1098/rsob.230438

Hany K M Dweck, Claire E Rutledge

{"title":"The subapical labial sensory organ of spotted lanternfly Lycorma delicatula.","authors":"Hany K M Dweck, Claire E Rutledge","doi":"10.1098/rsob.230438","DOIUrl":"10.1098/rsob.230438","url":null,"abstract":"Deciphering how spotted lanternfly (SLF), an invasive polyphagous planthopper in North America, engages with its environment is a pressing issue with fundamental biological significance and economic importance. This interaction primarily depends on olfaction. However, the cellular basis of olfaction in SLF remains elusive. Here we investigate the neuronal and functional organization of the subapical labial sensory organ using scanning electron microscopy and electrophysiological recordings. This organ is believed to supply planthoppers with crucial sensory information that influences their subsequent feeding behaviour. We find in SLF that this organ comprises two identical placoid sensilla, each housing two distinct neurons. The A neuron displays a remarkable sensitivity to changes in airflow speed. Importantly, the same neuron also exhibits robust excitatory responses exclusively to three aldehydes out of a diverse pool of 85 tested odorants and inhibitory responses to 62 other odorants. By contrast, the B neuron solely serves as an olfactory detector, showing strong excitatory responses to 17 odorants and inhibitory responses to only three. The results provide a potential cellular basis for the behavioural responses of SLF to its ecologically relevant stimuli. Our study also identifies new odorants that may be useful for managing this serious pest.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 3","pages":"230438"},"PeriodicalIF":4.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10965328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140294124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-1202 acts as anti-oncomiR in myeloid leukaemia by down-modulating GATA-1_S expression. miR-1202 通过下调 GATA-1S 的表达，在髓细胞白血病中发挥抗肿瘤坏死因子的作用。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-02-01 Epub Date: 2024-02-14 DOI: 10.1098/rsob.230319

Raffaele Sessa, Silvia Trombetti, Alessandra Lo Bianco, Giovanni Amendola, Rosa Catapano, Elena Cesaro, Fara Petruzziello, Maria D'Armiento, Giuseppe Maria Maruotti, Giuseppe Menna, Paola Izzo, Michela Grosso

{"title":"miR-1202 acts as anti-oncomiR in myeloid leukaemia by down-modulating GATA-1S expression.","authors":"Raffaele Sessa, Silvia Trombetti, Alessandra Lo Bianco, Giovanni Amendola, Rosa Catapano, Elena Cesaro, Fara Petruzziello, Maria D'Armiento, Giuseppe Maria Maruotti, Giuseppe Menna, Paola Izzo, Michela Grosso","doi":"10.1098/rsob.230319","DOIUrl":"10.1098/rsob.230319","url":null,"abstract":"Transient abnormal myelopoiesis (TAM) is a Down syndrome-related pre-leukaemic condition characterized by somatic mutations in the haematopoietic transcription factor GATA-1 that result in exclusive production of its shorter isoform (GATA-1S). Given the common hallmark of altered miRNA expression profiles in haematological malignancies and the pro-leukaemic role of GATA-1S, we aimed to search for miRNAs potentially able to modulate the expression of GATA-1 isoforms. Starting from an in silico prediction of miRNA binding sites in the GATA-1 transcript, miR-1202 came into our sight as potential regulator of GATA-1 expression. Expression studies in K562 cells revealed that miR-1202 directly targets GATA-1, negatively regulates its expression, impairs GATA-1S production, reduces cell proliferation, and increases apoptosis sensitivity. Furthermore, data from TAM and myeloid leukaemia patients provided substantial support to our study by showing that miR-1202 down-modulation is accompanied by increased GATA-1 levels, with more marked effects on GATA-1S. These findings indicate that miR-1202 acts as an anti-oncomiR in myeloid cells and may impact leukaemogenesis at least in part by down-modulating GATA-1S levels.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 2","pages":"230319"},"PeriodicalIF":5.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NFIL3 contributes to cytotoxic T lymphocyte-mediated killing. NFIL3 有助于细胞毒性 T 淋巴细胞介导的杀伤作用。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-02-01 Epub Date: 2024-02-28 DOI: 10.1098/rsob.230456

Tiphaine Douanne, Katharina Strege, Martin Del Castillo Velasco-Herrera, Adam M Rochussen, David J Adams, Gillian M Griffiths

{"title":"NFIL3 contributes to cytotoxic T lymphocyte-mediated killing.","authors":"Tiphaine Douanne, Katharina Strege, Martin Del Castillo Velasco-Herrera, Adam M Rochussen, David J Adams, Gillian M Griffiths","doi":"10.1098/rsob.230456","DOIUrl":"10.1098/rsob.230456","url":null,"abstract":"Cytotoxic T lymphocytes (CTLs) are key effectors of the adaptive immune system that recognize and eliminate virally infected and cancerous cells. In naive CD8+ T cells, T-cell receptor (TCR) engagement drives a number of transcriptional, translational and proliferation changes over the course of hours and days leading to differentiation into CTLs. To gain a better insight into this mechanism, we compared the transcriptional profiles of naive CD8+ T cells to those of activated CTLs. To find new regulators of CTL function, we performed a selective clustered regularly interspaced short palindromic repeats (CRISPR) screen on upregulated genes and identified nuclear factor IL-3 (NFIL3) as a potential regulator of cytotoxicity. Although NFIL3 has established roles in several immune cells including natural killer, Treg, dendritic and CD4+ T cells, its function in CD8+ CTLs is less well understood. Using CRISPR/Cas9 editing, we found that removing NFIL3 in CTLs resulted in a marked decrease in cytotoxicity. We found that in CTLs lacking NFIL3 TCR-induced extracellular signal-regulated kinase phosphorylation, immune synapse formation and granule release were all intact while cytotoxicity was functionally impaired in vitro. Strikingly, NFIL3 controls the production of cytolytic proteins as well as effector cytokines. Thus, NFIL3 plays a cell intrinsic role in modulating cytolytic mechanisms in CTLs.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 2","pages":"230456"},"PeriodicalIF":5.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10898977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level. 在裂殖酵母中结合单分子显微镜和膨胀显微镜，在纳米结构水平上观察蛋白质结构。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-02-01 Epub Date: 2024-02-07 DOI: 10.1098/rsob.230414

Ilijana Vojnovic, Oliver D Caspari, Mehmet Ali Hoşkan, Ulrike Endesfelder

{"title":"Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level.","authors":"Ilijana Vojnovic, Oliver D Caspari, Mehmet Ali Hoşkan, Ulrike Endesfelder","doi":"10.1098/rsob.230414","DOIUrl":"10.1098/rsob.230414","url":null,"abstract":"In this work, we have developed an expansion microscopy (ExM) protocol that combines ExM with photoactivated localization microscopy (ExPALM) for yeast cell imaging, and report a robust protocol for single-molecule and expansion microscopy of fission yeast, abbreviated as SExY. Our optimized SExY protocol retains about 50% of the fluorescent protein signal, doubling the amount obtained compared to the original protein retention ExM (proExM) protocol. It allows for a fivefold, highly isotropic expansion of fission yeast cells, which we carefully controlled while optimizing protein yield. We demonstrate the SExY method on several exemplary molecular targets and explicitly introduce low-abundant protein targets (e.g. nuclear proteins such as cbp1 and mis16, and the centromere-specific histone protein cnp1). The SExY protocol optimizations increasing protein yield could be beneficial for many studies, when targeting low abundance proteins, or for studies that rely on genetic labelling for various reasons (e.g. for proteins that cannot be easily targeted by extrinsic staining or in case artefacts introduced by unspecific staining interfere with data quality).","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 2","pages":"230414"},"PeriodicalIF":5.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10846934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139697977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Baculovirus entry into the central nervous system of Spodoptera exigua caterpillars is independent of the viral protein tyrosine phosphatase. 杆状病毒进入旋毛虫中枢神经系统与病毒蛋白酪氨酸磷酸酶无关。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-02-01 Epub Date: 2024-02-21 DOI: 10.1098/rsob.230278

Simone N Gasque, Yue Han, Iris van der Ham, Dorothy van Leeuwen, Monique M van Oers, Alexander Haverkamp, Vera I D Ros

{"title":"Baculovirus entry into the central nervous system of Spodoptera exigua caterpillars is independent of the viral protein tyrosine phosphatase.","authors":"Simone N Gasque, Yue Han, Iris van der Ham, Dorothy van Leeuwen, Monique M van Oers, Alexander Haverkamp, Vera I D Ros","doi":"10.1098/rsob.230278","DOIUrl":"10.1098/rsob.230278","url":null,"abstract":"Neuroparasitism concerns the hostile take-over of a host's nervous system by a foreign invader, in order to alter the behaviour of the host in favour of the parasite. One of the most remarkable cases of parasite-induced host behavioural manipulation comprises the changes baculoviruses induce in their caterpillar hosts. Baculoviruses may manipulate caterpillar behaviour in two ways: hyperactivity (increased movement in the horizontal plane) and/or tree-top disease (movement to elevated levels in the vertical plane). Those behavioural changes are followed by liquefaction and death of the caterpillar. In Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-infected Spodoptera exigua caterpillars, an enzymatic active form of the virally encoded protein tyrosine phosphatase (PTP) is needed for the expression of hyperactivity from 3 days post infection (dpi). Using eGFP-expressing recombinant AcMNPV strains, we show that infection of the caterpillar's central nervous system (CNS) can be observed primarily from 3 dpi onwards. In addition, we demonstrate that the structural and enzymatic function of PTP does not play a role in infection of the CNS. Instead we show that the virus entered the CNS via the trachea, progressing caudally to frontally through the CNS and that the infection progressed from the outermost cell layers towards the inner cell layers of the CNS, in a PTP independent manner. These findings help to further understand parasitic manipulation and the mechanisms by which neuroparasites infect the host nervous system to manipulate host behaviour.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 2","pages":"230278"},"PeriodicalIF":5.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10878822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139913203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibiting responses under the watch of a recently synchronized peer increases self-monitoring: evidence from functional near-infrared spectroscopy. 在最近同步的同伴注视下抑制反应会增强自我监控能力：功能性近红外光谱的证据。

IF 4.5 3区生物学

Open Biology Pub Date : 2024-02-01 Epub Date: 2024-02-21 DOI: 10.1098/rsob.230382

R Moffat, N Caruana, E S Cross

{"title":"Inhibiting responses under the watch of a recently synchronized peer increases self-monitoring: evidence from functional near-infrared spectroscopy.","authors":"R Moffat, N Caruana, E S Cross","doi":"10.1098/rsob.230382","DOIUrl":"10.1098/rsob.230382","url":null,"abstract":"Developing motor synchrony with a peer (through interventions such as the mirror game) can yield collaborative, cognitive and social benefits. However, it is also well established that observation by an audience can improve cognition. The combined and relative advantages offered by motor synchronization and audience effects are not yet understood. It is important to address this gap to determine the extent to which synchronizing activities might interact with the positive effects of an audience. In this preregistered study, we investigate the extent to which response inhibition may be improved when observed by a peer after motor synchronization with this peer. We compare behavioural and cortical (functional near-infrared spectroscopy; fNIRS) measures of inhibition between synchronized and non-synchronized dyads and find that the presence of a synchronized peer-audience introduces a speed-accuracy trade-off, consisting of slower reaction times and improved accuracy. This co-occurs with cortical activation in bilateral inferior frontal and middle prefrontal cortices, which are implicated in monitoring and maintenance of social alignment. Our findings have implications for carers and support people, who may benefit from synchronizing activities for rehabilitating inhibition and social skills in clinical settings.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 2","pages":"230382"},"PeriodicalIF":4.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10878812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139913204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models. 从肠道到肠外：沙门氏菌进入因子在小鼠模型中感染后显示出不同的转录模式。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-01-01 Epub Date: 2024-01-17 DOI: 10.1098/rsob.230312

Michaël Koczerka, Isabelle Lantier, Marie Morillon, Justine Deperne, Camille D Clamagirand, Isabelle Virlogeux-Payant, Olivier Grépinet

{"title":"From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models.","authors":"Michaël Koczerka, Isabelle Lantier, Marie Morillon, Justine Deperne, Camille D Clamagirand, Isabelle Virlogeux-Payant, Olivier Grépinet","doi":"10.1098/rsob.230312","DOIUrl":"10.1098/rsob.230312","url":null,"abstract":"The infectious process of bacteria of the genus Salmonella requires the finely regulated use of various virulence factors. Among them, the type 3 secretion system-1 (T3SS-1) and the Rck and PagN invasins are involved in the internalization of the pathogen within eukaryotic cells, but their precise role in the host and in the pathogenic process is still poorly understood. In this study, we aimed to determine the kinetics of expression of these entry factors in a typhoid fever-like and a gastroenteritis model in mice by in vivo imaging using bioluminescent Salmonella Typhimurium reporter strains carrying chromosomal transcriptional fusions. Only pagN and T3SS-1 transcription has been clearly identified. Independently of the pathological model, the caecum was identified as the main transcription site of both pagN and the T3SS-1-encoding gene both at early and late stages of the infection. An intense transcription of pagN was also observed in deep organs in the typhoid fever-like model, while that of T3SS-1 remained quite sporadic in these organs, and mainly focused on the intestine all along the infection. This work will help to understand the respective role of these entry factors at the cellular level in the pathogenesis of Salmonella in vivo.","PeriodicalId":19629,"journal":{"name":"Open Biology","volume":"14 1","pages":"230312"},"PeriodicalIF":5.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10791514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139479154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fission yeast Wee1 is required for stable kinetochore-microtubule attachment. 裂殖酵母 Wee1 是稳定的动点心轴-微管连接所必需的。

IF 5.8 3区生物学

Open Biology Pub Date : 2024-01-01 Epub Date: 2024-01-03 DOI: 10.1098/rsob.230379

Masahiro Takado, Takaharu G Yamamoto, Yuji Chikashige, Tomohiro Matsumoto

引用次数: 0