Oral diseases最新文献_第9页

GalNAc-Conjugated siRNA Targeting Complement C3 Inhibits Osteoclast Activation in Periodontitis. 靶向补体 C3 的 GalNAc 结合 siRNA 可抑制牙周炎中破骨细胞的活化。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-10-29 DOI: 10.1111/odi.15170

Yingyi Chen, Yitong Liu, Zhongguo Fu, Junji Xu, Lijia Guo, Huiqing Cao, Liming Gan, Shan Gao, Yi Liu

{"title":"GalNAc-Conjugated siRNA Targeting Complement C3 Inhibits Osteoclast Activation in Periodontitis.","authors":"Yingyi Chen, Yitong Liu, Zhongguo Fu, Junji Xu, Lijia Guo, Huiqing Cao, Liming Gan, Shan Gao, Yi Liu","doi":"10.1111/odi.15170","DOIUrl":"10.1111/odi.15170","url":null,"abstract":"Objective: The complement cascade plays an important role in the inflammation amplification and tissue destruction of periodontitis. Importantly, complement C3 was proved to be the central element of complement cascade. Thus, targeting inhibition of C3 has become one of the focuses of treatment method development and exploration.Methods: The siRNAs targeting C3 were designed and screened for in vitro potency. The selected siRNA was conjugated to GalNAc (GalNAc-C3 siRNA) for liver-specific delivery. The mouse model of periodontitis was established by silk ligation. Stereomicroscopy, Micro-CT, histological and histochemical assessment, and immunofluorescence staining were performed to evaluate the level of bone destructive and osteoclast activity. The influence of GalNAc-C3 siRNA on inflammatory reactions was determined by qRT-PCR, ELISA, and flow cytometry.Results: GalNAc-C3 siRNA showed great in vivo potency and durability to silence hepatic C3 mRNA expression. GalNAc-C3 siRNA treatment could effectively inhibit the production of inflammatory cytokines (IL-17A, TNF-α, IL-6, and IFN-γ) and restrain Th17 differentiation. Importantly, the expression of RANKL and differentiation of osteoclast were inhibited by GalNAc-C3 siRNA.Conclusion: GalNAc-C3 siRNA could efficiently play a role in bone protection by inhibiting inflammatory responses and osteoclast activities. This therapeutic siRNA may become an effective treatment strategy for periodontitis.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"589-599"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning-based hyperspectral technique identifies metastatic lymph nodes in oral squamous cell carcinoma-A pilot study. 基于深度学习的高光谱技术识别口腔鳞状细胞癌的转移淋巴结--一项试点研究。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-07-15 DOI: 10.1111/odi.15067

Qingxiang Li, Xueyu Zhang, Jianyun Zhang, Hongyuan Huang, Liangliang Li, Chuanbin Guo, Wei Li, Yuxing Guo

{"title":"Deep learning-based hyperspectral technique identifies metastatic lymph nodes in oral squamous cell carcinoma-A pilot study.","authors":"Qingxiang Li, Xueyu Zhang, Jianyun Zhang, Hongyuan Huang, Liangliang Li, Chuanbin Guo, Wei Li, Yuxing Guo","doi":"10.1111/odi.15067","DOIUrl":"10.1111/odi.15067","url":null,"abstract":"Aims: To establish a system based on hyperspectral imaging and deep learning for the detection of cancer cells in metastatic lymph nodes.Main methods: The continuous sections of metastatic lymph nodes from 45 oral squamous cell carcinoma (OSCC) patients were collected. An improved ResUNet algorithm was established for deep learning to analyze the spectral curve differences between cancer cells and lymphocytes, and that between tumor tissue and normal tissue.Key findings: It was found that cancer cells, lymphocytes, and erythrocytes in the metastatic lymph nodes could be distinguished basing hyperspectral image, with overall accuracy (OA) as 87.30% and average accuracy (AA) as 85.46%. Cancerous area could be recognized by hyperspectral image and deep learning, and the average intersection over union (IOU) and accuracy were 0.6253 and 0.7692, respectively.Significance: This study indicated that deep learning-based hyperspectral techniques can identify tumor tissue in OSCC metastatic lymph nodes, achieving high accuracy of pathological diagnosis, high work efficiency, and reducing work burden. But these are preliminary results limited to a small sample.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"417-425"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Areca nut-induced AREG promote oral epithelial cell proliferation, migration, and EMT. 阿月浑子诱导的 AREG 可促进口腔上皮细胞增殖、迁移和 EMT。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-07-15 DOI: 10.1111/odi.15065

Ming Li, Zhenying Yuan, Zhangui Tang

{"title":"Areca nut-induced AREG promote oral epithelial cell proliferation, migration, and EMT.","authors":"Ming Li, Zhenying Yuan, Zhangui Tang","doi":"10.1111/odi.15065","DOIUrl":"10.1111/odi.15065","url":null,"abstract":"Objective: To analyze the biological effect and mechanism of areca nut extract (ANE) on human oral keratinocyte (HOK) cells.Materials and methods: The effect of gradient concentration of ANE on the proliferation activity of HOK cells was analyzed by cell counting kit-8 (CCK-8) assays. The differentially expressed genes between the ANE group and control group HOK cells were analyzed by second-generation transcriptome sequencing. Real-time PCR and western blot were, respectively, used to analyze the expression of AREG gene and protein in HOK cells. After AREG gene overexpression or knockdown, the proliferation, migration, and expression of proteins related to epithelial-mesenchymal transformation (EMT), MAPK signal pathway in HOK cells were, respectively, detected by CCK-8, wound healing, transwell, and western blot assays.Results: ANE (500 μg/mL) promoted the proliferation and migration of HOK cells, ANE (2 mg/mL) promoted the EMT of HOK cells, and ANE (50 mg/mL) inhibited the proliferation of HOK cells. AREG knockdown inhibited ANE-induced proliferation and migration of HOK cells, while AREG overexpression promoted the proliferation and migration of HOK cells. Western blot assay showed that ANE activated MAPK signal pathway by upregulating AREG protein in HOK cells.Conclusions: ANE promoted HOK cell proliferation, migration, and EMT by mediating AREG-MAPK signaling pathway.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"354-363"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin overcomes chemoresistance by regulating stemness via KLF4 in oral squamous cell carcinoma. 二甲双胍通过KLF4调节口腔鳞状细胞癌的干性来克服化疗耐药性

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-07-22 DOI: 10.1111/odi.15075

Tong Zhou, Xuefeng Zhang, Dan Yang, Weideng Wei, Jianguo Gan, Xiaoqiang Xia, Qianming Chen, Jian Jiang, Xiaodong Feng

{"title":"Metformin overcomes chemoresistance by regulating stemness via KLF4 in oral squamous cell carcinoma.","authors":"Tong Zhou, Xuefeng Zhang, Dan Yang, Weideng Wei, Jianguo Gan, Xiaoqiang Xia, Qianming Chen, Jian Jiang, Xiaodong Feng","doi":"10.1111/odi.15075","DOIUrl":"10.1111/odi.15075","url":null,"abstract":"Objective: Chemoresistance is a common event after chemotherapy, including oral squamous cell carcinoma (OSCC). Accumulated evidence suggests that the cancer stemness significantly contributes to therapy resistance. An unresolved question remains regarding how to effectively overcome OSCC chemoresistance by targeting stemness. This study aims to investigate the antitumor effect of metformin and clarify the potential molecular mechanisms.Methods: Cellular models resistant to chemotherapy were established, and their viability and sphere-forming ability were assessed using CCK-8 and soft agar formation assays, respectively. RNA-seq and Western blotting analyses were employed to delve into the molecular pathways. Furthermore, to corroborate the inhibitory effects of metformin and cisplatin at an animal level, a subcutaneous tumor transplantation model was instituted.Results: Metformin as a monotherapy exhibited inhibition of stemness traits via Krüppel-like factor 4 (KLF4). Metformin and cisplatin can synergically inhibit cell proliferation and induce cell apoptosis. Animal experiments confirmed the inhibitory effect of cisplatin and metformin on tumor in mice.Conclusion: Our study proposes a potential therapeutic approach of combining chemotherapy with metformin to overcome chemoresistance in OSCC.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"468-481"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An updated categorization of pathologic extraoral halitosis. 口外病理性口臭的最新分类。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-07-22 DOI: 10.1111/odi.15085

Xiao Xian Qian

引用次数: 0

Leveraging programmed cell death patterns to predict prognosis and therapeutic sensitivity in OSCC. 利用程序性细胞死亡模式预测 OSCC 的预后和治疗敏感性。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-09-24 DOI: 10.1111/odi.15139

An Wang, Chi Zhang, Yuhan Wang, Pengfei Diao, Jie Cheng

{"title":"Leveraging programmed cell death patterns to predict prognosis and therapeutic sensitivity in OSCC.","authors":"An Wang, Chi Zhang, Yuhan Wang, Pengfei Diao, Jie Cheng","doi":"10.1111/odi.15139","DOIUrl":"10.1111/odi.15139","url":null,"abstract":"Objectives: Intricate associations between programmed cell death (PCD) and cancer development and treatment outcomes have been increasingly appreciated. Here, we integrated 12 PCD patterns to construct a novel biomarker, cell death index (CDI), for oral squamous cell carcinoma (OSCC) prognostication and therapeutic prediction.Materials and methods: Univariate Cox regression, Kaplan-Meier survival, and LASSO analyses were performed to construct the CDI. A nomogram combining CDI and selected clinicopathological parameters was established by multivariate Cox regression. The associations between CDI and immune landscape and therapeutic sensitivity were estimated. Single-cell RNA-seq data of OSCC was used to infer CDI genes in selected cell types and determine their expression along cell differentiation trajectory.Results: Ten selected PCD genes derived a novel prognostic signature for OSCC. The predictive prognostic performance of CDI and nomogram was robust and superior across multiple independent patient cohorts. CDI was negatively associated with tumor-infiltrating immune cell abundance and immunotherapeutic outcomes. Moreover, scRNA-seq data reanalysis revealed that GSDMB, IL-1A, PRKAA2, and SFRP1 from this signature were primarily expressed in cancer cells and involved in cell differentiation.Conclusions: Our findings established CDI as a novel powerful predictor for prognosis and therapeutic response for OSCC and suggested its potential involvement in cancer cell differentiation.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"452-467"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital mandibular appendicular nodule associated with a cervical/submandibular mass. 先天性下颌骨阑尾结节伴有颈部/下颌骨肿块。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-10-29 DOI: 10.1111/odi.15132

Alfio José Tincani, Gustavo de Souza Vieira, Luccas Lavareze, João Figueira Scarini, Carolina Emerick, Pedro Deak de Almeida, Tiago Antônio Baldasso, André Del Negro, André Luís Maion Casarim, Erika Said Abu Egal, Albina Altemani, Fernanda Viviane Mariano

引用次数: 0

Imiquimod for the treatment of oral leukoplakia: A two-center retrospective study. 咪喹莫特治疗口腔白斑病：双中心回顾性研究

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-07-15 DOI: 10.1111/odi.15069

Herve Sroussi, Alessandro Villa, Malak Adel Alhadlaq, Kentaro Ikeda, Sivappiriyai Veluppillai, Nathaniel Treister, Anette Vistoso Monreal, Piamkamon Vacharotayangul, Michele Lodolo, Sook-Bin Woo

{"title":"Imiquimod for the treatment of oral leukoplakia: A two-center retrospective study.","authors":"Herve Sroussi, Alessandro Villa, Malak Adel Alhadlaq, Kentaro Ikeda, Sivappiriyai Veluppillai, Nathaniel Treister, Anette Vistoso Monreal, Piamkamon Vacharotayangul, Michele Lodolo, Sook-Bin Woo","doi":"10.1111/odi.15069","DOIUrl":"10.1111/odi.15069","url":null,"abstract":"Objectives: We aimed to assess the effectiveness of the use of topical imiquimod for the management of oral leukoplakia (OL).Methods: This was a retrospective study. Medical chart reviews were conducted to identify patients with biopsy-proven OL treated with topical 5% imiquimod. Data included OL characteristics, histopathological diagnosis, treatment outcome, and adverse events (AEs). Treatment response was assessed by measuring the percentage reduction in the size of OL lesions.Results: 33 patients (51.5% females; median age: 65 years) with 38 lesions were included. OLs were either localized (23.7%) or multifocal lesions (76.3%), with the majority on the gingiva (86.8%). Pretreatment histopathological diagnoses were dysplasia in 84.2% and nonreactive hyperkeratosis in 15.8%. Most regimens consisted of 60-minute applications, 5-days-a-week, for 6 weeks. At the end of treatment, 81.6% of 38 lesions showed a reduction in size with 68.4% exhibiting ≥50% reduction in size, and 42.1% exhibiting complete resolution. Application site reactions were the most common with pain/soreness/sensitivity occurring in 86.8%. Fatigue was the most frequently reported systemic AE (28.9%).Conclusion: Two-thirds of OL lesions had ≥50% reduction in size. Most AEs were temporary and resolved upon treatment discontinuation. Prospective studies are needed to further assess Imiquimod's effectiveness in OL management.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"444-451"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comment on Kittichet et al. (doi: 10.1111/odi.14905). 对 Kittichet 等人的评论（doi: 10.1111/odi.14905）。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-07-22 DOI: 10.1111/odi.15089

Efsun Somay, Erkan Topkan, Ugur Selek, Berrin Pehlivan

引用次数: 0

Efficacy of Combined vs. Monotherapy in Oral Lichen Planus: A Randomized Clinical Trial. 口腔扁平苔藓联合疗法与单一疗法的疗效对比：随机临床试验

IF 2.9 3区医学

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-10-22 DOI: 10.1111/odi.15151

Rocio Rodriguez-Galvez, Asta Tvarijonaviciute, Camila Peres-Rubio, Pia Lopez-Jornet

{"title":"Efficacy of Combined vs. Monotherapy in Oral Lichen Planus: A Randomized Clinical Trial.","authors":"Rocio Rodriguez-Galvez, Asta Tvarijonaviciute, Camila Peres-Rubio, Pia Lopez-Jornet","doi":"10.1111/odi.15151","DOIUrl":"10.1111/odi.15151","url":null,"abstract":"Objectives: The aim of this study was to compare the topical therapeutic efficacy of triamcinolone acetonide (TA) (0.2%) plus hyaluronic acid (HA) (0.1%) versus monotherapy in patients with symptomatic oral lichen planus (OLP), as well as to investigate the oxidative stress of saliva under the different treatments.Materials and methods: Sixty OLP patients were included in a randomized, double-blind, singlecenter study with a treatment duration of 28 days and 3-month follow-up period. Participants were randomized into three groups: Group I (TA + HA), Group II (TA), and Group III (HA). Treatment efficacy was assessed by means of Thongprasom scale, Oral Health Impact Profile-14 (OHIP-14), and visual analog scale (VAS). In addition, biochemical analyses were performed in order to determine the level of antioxidant biomarkers in saliva, including superoxide dismutase (SOD), glutathione (GSH), and total antioxidant capacity (TAC).Results: All treatments seem to exhibit a significant effect in accordance with Thongprasom scale (p < 0.001), VAS reduction (p < 0.001), and OHIP14 (p < 0.05), which maintains over time. No significant changes in salivary oxidative stress in any of the three groups occurred.Conclusions: The results exhibited a significant improvement in the treated patients in all three groups. There were no significant changes in salivary stress biomarkers under treatment condition.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"522-531"},"PeriodicalIF":2.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142471643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0