Oral diseases最新文献_第9页

Comment on "Site-specific radiation dosage and implant survival in oral cancer patients: A cohort study". 放疗或化放疗后造成植入物损失的潜在因素。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-02-20 DOI: 10.1111/odi.14889

Erkan Topkan, Efsun Somay, Uğur Selek

引用次数: 0

Virucidal effect of mouthwash on acyclovir-resistant herpes simplex virus. 漱口水对阿昔洛韦耐药单纯疱疹病毒的杀病毒作用。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2023-12-27 DOI: 10.1111/odi.14843

M Bar Ilan, S Dovrat, R Cohen, M Georgaki, E Papadopoulou, N G Nikitakis, N Yarom

{"title":"Virucidal effect of mouthwash on acyclovir-resistant herpes simplex virus.","authors":"M Bar Ilan, S Dovrat, R Cohen, M Georgaki, E Papadopoulou, N G Nikitakis, N Yarom","doi":"10.1111/odi.14843","DOIUrl":"10.1111/odi.14843","url":null,"abstract":"Objectives: The symptoms of herpes simplex viruses type 1 (HSV-1) infections might be severe and persistent in immunocompromised patients in whom they reactivate at a high frequency. The development of Acyclovir (ACV) resistant strains due to long-term treatment with antiviral agents in those patients is not uncommon. The aim of the present study was to assess the virucidal effect of commercially available mouthwashes against ACV-resistant HSV-1 strains.Materials and methods: Two acyclovir-resistant HSV-1 strains were exposed for 30 s to essential oil-based (Listerine Fresh Burst® and Listerine Zero®), chlorhexidine gluconate 0.2% (Hexidyl®) and povidone-iodine 7.5% (Betadine Gargle®) mouthwashes. Loss of virus infectivity was determined by means of plaque reduction assays in a cell culture system.Results: All 4 of the tested solutions significantly reduced virus infectivity, with the essential oil-based and povidone-iodine mouthwashes being slightly more efficacious, compared to chlorhexidine.Conclusion: The findings of this analysis revealed that the tested oral rinses demonstrated in-vitro antiviral activity against ACV-resistant HSV. Comparative clinical trials are required to establish the clinical effectiveness of daily use of oral rinses in reducing the appearance of oral HSV lesions in immunocompromised patients.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4762-4766"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139049108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between periodontal disease and malignant soft tissue sarcomas. 牙周病与恶性软组织肉瘤的关系

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-01-08 DOI: 10.1111/odi.14853

Takatoshi Hiroshimaya, Yusuke Fujimoto, Hiromi Sasaki, Toshihiro Motoi, Emi Nagata, Noboru Taniguchi, Takahiko Oho

{"title":"Association between periodontal disease and malignant soft tissue sarcomas.","authors":"Takatoshi Hiroshimaya, Yusuke Fujimoto, Hiromi Sasaki, Toshihiro Motoi, Emi Nagata, Noboru Taniguchi, Takahiko Oho","doi":"10.1111/odi.14853","DOIUrl":"10.1111/odi.14853","url":null,"abstract":"Objective: Malignant soft tissue sarcoma (MSTS) is a rare disease, but is seen in patients undergoing orthopedic surgery. Although the association of periodontal disease with various cancers occurring in the oral cavity, gastrointestinal tract, lungs, and prostate, has been reported, the association between periodontal disease and MSTS remains unclear. This study investigated the association between periodontal disease and MSTS in patients undergoing orthopedic surgery.Subjects and methods: One hundred fifteen patients who underwent orthopedic surgery between 2017 and 2021 were retrospectively enrolled (mean age = 66.8 ± 10.7 years). The patient background was adjusted by the propensity score (PS). Subsequently, the association of periodontal disease with MSTS was analyzed using PS inverse probability of treatment weighting (IPTW). Periodontal status was determined by evaluating the periodontal inflamed surface area, which was calculated by measuring the periodontal probing pocket depth and detecting bleeding on probing.Results: Multivariate logistic regression analysis after adjustment by the PS showed that severe periodontitis was significantly associated with MSTS (odds ratio 2.81, p = 0.04). Furthermore, IPTW showed that severe periodontitis was significantly associated with MSTS (odds ratio 3.21, p = 0.01).Conclusion: The results indicate an association between periodontal inflammation and MSTS.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4731-4739"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139403915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of differentiated dysplasia as a variant of oral epithelial dysplasia. 诊断分化性发育不良是口腔上皮发育不良的一种变异。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-01-08 DOI: 10.1111/odi.14846

Anne-Sophie Becker, Maximiliane Holm, Jan Liese, Nadja Engel, Annette Helene Zimpfer

{"title":"Diagnosis of differentiated dysplasia as a variant of oral epithelial dysplasia.","authors":"Anne-Sophie Becker, Maximiliane Holm, Jan Liese, Nadja Engel, Annette Helene Zimpfer","doi":"10.1111/odi.14846","DOIUrl":"10.1111/odi.14846","url":null,"abstract":"Objectives: The World Health Organization's definition of oral epithelial dysplasia includes differentiated dysplasia, which is defined by purely architectural abnormalities of oral mucosa without cytological changes. We analysed differentiated dysplasia's frequency, progression risk and correlation with oral brush cytology.Materials and methods: Cytoarchitectural criteria and expression patterns of keratin 13/17 and ki67 were studied in oral biopsies clinically diagnosed with leukoplakia. Biopsies were assessed for dysplasia and its grade. Available brush cytology findings were obtained from clinical records.Results: We included 159 biopsies from 112 patients (33% differentiated dysplasia; 27% keratosis without dysplasia; oral epithelial dysplasia with atypia of mild, moderate and severe degree including invasive cancers in 9%, 8% and 7%, respectively). Keratin 13 loss and keratin 17 gain were higher in differentiated-dysplasia cases (p < 0.0001), which had the highest hypergranulosis frequency. Keratin 17 expression was associated with higher malignant-transformation rates (p = 0.0028). The transformation rate and time were comparable between dysplasia with atypia and differentiated-dysplasia cases, which had higher progression rates and shorter time periods than keratosis cases without dysplasia (p = 0.08). Cytology prior to differentiated dysplasia all indicated normal oral mucosa.Conclusions: Keratin 17 but not oral brush cytology can help identify patients with differentiated dysplasia with higher risk for malignant transformation.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4185-4194"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139403916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Notch signaling regulates mineralization via microRNA modulation in dental pulp stem cells. Notch信号通过微RNA调节牙髓干细胞的矿化。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-01-19 DOI: 10.1111/odi.14868

Promphakkon Kulthanaamondhita, Chatvadee Kornsuthisopon, Ajjima Chansaenroj, Ravipha Suwittayarak, Voraphat Trachoo, Jeeranan Manokawinchoke, Seung-Cheol Lee, Hiroshi Egusa, Jin Man Kim, Thanaphum Osathanon

{"title":"Notch signaling regulates mineralization via microRNA modulation in dental pulp stem cells.","authors":"Promphakkon Kulthanaamondhita, Chatvadee Kornsuthisopon, Ajjima Chansaenroj, Ravipha Suwittayarak, Voraphat Trachoo, Jeeranan Manokawinchoke, Seung-Cheol Lee, Hiroshi Egusa, Jin Man Kim, Thanaphum Osathanon","doi":"10.1111/odi.14868","DOIUrl":"10.1111/odi.14868","url":null,"abstract":"Objectives: This study investigated the miRNA expression profile in Notch-activated human dental stem pulp stem cells (DPSCs) and validated the functions of miRNAs in modulating the odonto/osteogenic properties of DPSCs.Methods: DPSCs were treated with indirect immobilized Jagged1. The miRNA expression profile was examined using NanoString analysis. Bioinformatic analysis was performed, and miRNA expression was validated. Odonto/osteogenic differentiation was examined using alkaline phosphatase staining, Alizarin Red S staining, as well as odonto/osteogenic-related gene and protein expression.Results: Fourteen miRNAs were differentially expressed in Jagged1-treated DPSCs. Pathway analysis revealed that altered miRNAs were associated with TGF-β, Hippo, ErbB signalling pathways, FoxO and Ras signalling. Target prediction analysis demonstrated that 7604 genes were predicted to be targets for these altered miRNAs. Enrichment analysis revealed relationships to various DNA bindings. Among differentially expressed miRNA, miR-296-3p and miR-450b-5p were upregulated under Jagged1-treated conditions. Overexpression of miR-296-3p and miR-450b-5p enhanced mineralization and upregulation of odonto/osteogenic-related genes, whereas inhibition of these miRNAs revealed opposing results. The miR-296-3p and miR-450b-5p inhibitors attenuated the effects of Jagged1-induced mineralization in DPSCs.Conclusions: Jagged-1 promotes mineralization in DPSCs that are partially regulated by miRNA. The novel understanding of these miRNAs could lead to innovative controlled mechanisms that can be applied to modulate biology-targeted dental materials.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4547-4557"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139502978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periodontitis and systemic parameters in chronic kidney disease: Systematic review and meta-analysis. 牙周炎与慢性肾脏病的系统参数：系统回顾和荟萃分析。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-05-09 DOI: 10.1111/odi.14981

Suélem Maria Santana Pinheiro Ferreira, Isaac Suzart Gomes-Filho, Maria da Conceição Nascimento Costa, Simone Seixas da Cruz, Carolina Lara Neves, Carlos Alberto Lima da Silva, Kaio Vinicius Freitas de Andrade, Marcela Beatriz Aguiar Moreira, Alexandre Marcelo Hintz, Maria Isabel Pereira Vianna

{"title":"Periodontitis and systemic parameters in chronic kidney disease: Systematic review and meta-analysis.","authors":"Suélem Maria Santana Pinheiro Ferreira, Isaac Suzart Gomes-Filho, Maria da Conceição Nascimento Costa, Simone Seixas da Cruz, Carolina Lara Neves, Carlos Alberto Lima da Silva, Kaio Vinicius Freitas de Andrade, Marcela Beatriz Aguiar Moreira, Alexandre Marcelo Hintz, Maria Isabel Pereira Vianna","doi":"10.1111/odi.14981","DOIUrl":"10.1111/odi.14981","url":null,"abstract":"Objective: To perform a systematic review with meta-analysis to assess recent scientific evidence on the association between periodontitis and systemic parameters/conditions in individuals with chronic kidney disease (CKD).Materials and methods: The search for studies was performed in MedLine/PubMed, Scopus, Web of Science, and BIREME databases. Reference lists of selected articles were also searched. Studies with different epidemiological designs evaluating the influence of exposure to periodontitis on serum markers and mortality in individuals with CKD were eligible for inclusion. Three independent reviewers performed the article selection and data extraction. The assessment of methodological quality used the adapted Newcastle Ottawa Scale. Random effects meta-analysis was performed to calculate association measurements and 95% confidence intervals.Results: In total, 3053 records were identified in the database search, with only 25 studies meeting the eligibility criteria and, of these, 10 studies contributed data for meta-analysis. Using a random-effects model, periodontitis was associated with hypoalbuminemia (PRunadjusted = 2.47; 95%CI:1.43-4.26), with high levels of C-reactive protein (PRunadjusted = 1.35; 95%CI%:1.12-1.64), death from cardiovascular disease (RRunadjusted = 2.29; 95%CI:1.67-3.15) and death from all causes (RRunadjusted = 1.73; 95%CI:1.32-2.27).Conclusions: The findings of this review validated a positive association between periodontitis and serum markers and mortality data in individuals with CKD.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4078-4086"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140892023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of six novel mutations in EDA from 20 hypohidrotic ectodermal dysplasia families. 从20个发育不全外胚层发育不良家族中鉴定出EDA中的六种新型突变。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2023-12-21 DOI: 10.1111/odi.14838

Qin Xing, Qimin Zhou, Hongyan Li, Zhongjie Wang, Shun Li, Jiayu Wu, Huimin Zhu, Desheng Liang, Zhuo Li, Lingqian Wu

{"title":"Identification of six novel mutations in EDA from 20 hypohidrotic ectodermal dysplasia families.","authors":"Qin Xing, Qimin Zhou, Hongyan Li, Zhongjie Wang, Shun Li, Jiayu Wu, Huimin Zhu, Desheng Liang, Zhuo Li, Lingqian Wu","doi":"10.1111/odi.14838","DOIUrl":"10.1111/odi.14838","url":null,"abstract":"Objective: To investigate the genetic causes of 22 patients with clinically high suspicion of X-linked hypohidrotic ectodermal dysplasia from 20 unrelated Chinese families, expand the spectrum of ectodysplasin-A mutations, and provide more evidence for variants of uncertain significance.Subjects and methods: Whole-exome sequencing was performed and potentially pathogenic variants were verified by Sanger sequencing. Western blotting, real-time PCR and immunofluorescence analyses were performed to investigate the preliminary functions of the candidate variants.Results: Nineteen ectodysplasin-A variants were identified, six of which were not previously reported. Among these variants, we identified a patient who carried two mutations in ectodysplasin-A and exhibited more severe phenotypes. Additionally, mutant protein expression levels decreased, whereas mRNA transcription levels increased. Cellular sublocalisation of the variants located in the tumour necrosis factor homologous domain showed that the proteins accumulated in the nucleus, whereas wild-type proteins remained in the cell membrane. A rare indel variant and two classical splicing variants that lead to exon 7 skipping were detected.Conclusions: This study provides definitive diagnoses for 20 families with suspected X-linked hypohidrotic ectodermal dysplasia and additional information on clinical heterogeneity and genotype-phenotype relationships.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4608-4619"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetics of neutrophil extracellular traps and cytokines in oral mucositis and Candida infection. 口腔粘膜炎和念珠菌感染时中性粒细胞胞外捕获物和细胞因子的动力学。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-01-04 DOI: 10.1111/odi.14857

José Alcides Almeida de Arruda, Sicília Rezende Oliveira, Fernanda Vieira Heimlich, Bárbara Maria de Amorim-Santos, Ayda Henriques Schneider, Ana Carolina Velasco Pondé de Sena, Karla Emília de Sá Rodrigues, Soraia Macari, Daniele Glória Souza, Denise Vieira Travassos, Lucas Guimarães Abreu, Tarcília Aparecida Silva, Ricardo Alves Mesquita

{"title":"Kinetics of neutrophil extracellular traps and cytokines in oral mucositis and Candida infection.","authors":"José Alcides Almeida de Arruda, Sicília Rezende Oliveira, Fernanda Vieira Heimlich, Bárbara Maria de Amorim-Santos, Ayda Henriques Schneider, Ana Carolina Velasco Pondé de Sena, Karla Emília de Sá Rodrigues, Soraia Macari, Daniele Glória Souza, Denise Vieira Travassos, Lucas Guimarães Abreu, Tarcília Aparecida Silva, Ricardo Alves Mesquita","doi":"10.1111/odi.14857","DOIUrl":"10.1111/odi.14857","url":null,"abstract":"Objective: This study investigated the concentrations of neutrophil extracellular traps (NET) and salivary cytokines (IL-1β, IL-6, IL-8/CXCL8, TNF, and TGF-β1) in patients undergoing chemotherapy and their associations with oral mucositis (OM) and Candida infection.Materials and methods: This prospective longitudinal study performed at a Brazilian service included 60 adults diagnosed with hematolymphoid diseases. Saliva samples were collected on days D0, D3, D10, and D15. Cytokines were analyzed by ELISA and NET formation by identification of the myeloperoxidase-DNA complex. Oral Candida spp. was cultured.Results: OM occurred in 43.3% of patients and oral candidiasis in 20%. However, 66% of individuals had positive cultures for C. albicans. Higher concentrations of IL-6, IL-8/CXCL8, and TNF and lower concentrations of TGF-β1 were observed in patients with OM. C. albicans infection contributed to the increase in IL-8/CXCL8, TGF-β1, and TNF. Individuals with OM or with oral candidiasis had significant reductions in NET formation. In contrast, individuals with C. albicans and with concomitant C. albicans and OM exhibited higher NET formation.Conclusion: The kinetics of cytokine levels and NET formation in chemotherapy-induced OM appears to be altered by Candida infection, even in the absence of clinical signs of oral candidiasis.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4751-4761"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139098402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALDH3A1 upregulation inhibits neutrophils N2 polarization and halts oral cancer growth. ALDH3A1上调可抑制中性粒细胞的N2极化并阻止口腔癌的生长。

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-01-15 DOI: 10.1111/odi.14863

Ying He, Yi Qu, Shan Jin, Yongfeng Zhang, Lizheng Qin

{"title":"ALDH3A1 upregulation inhibits neutrophils N2 polarization and halts oral cancer growth.","authors":"Ying He, Yi Qu, Shan Jin, Yongfeng Zhang, Lizheng Qin","doi":"10.1111/odi.14863","DOIUrl":"10.1111/odi.14863","url":null,"abstract":"Objectives: Tumor-associated neutrophils (TANs) are among the most abundant inflammatory cells in tumor microenvironment (TME). Aldehyde dehydrogenase 3A1 (ALDH3A1) is significantly reduced in oral squamous cell carcinoma (OSCC), ALDH3A1 overexpression suppresses tumorigenesis by inhibiting inflammation. This study investigated the relationship and mechanisms underlying the crosstalk between ALDH3A1 and TANs in OSCC.Materials and methods: Immunohistochemistry and immunofluorescence were performed to investigate the abundance of TANs and the expression of ALDH3A1. dHL-60 were induced with tumor-conditioned media and recombinant IL-6/IL-8. The expression of key proteins in PI3K/AKT/NF-κB pathway were detected by RT-PCR and western blot. A xenograft model was utilized to examine the effect of ALDH3A1 on tumorigenicity and polarization of TANs.Results: In patients with OSCC, TANs significantly increased and were associated with a worse prognosis. Additionally, ALDH3A1 negatively correlated with TANs infiltration and especially the N2 phenotype which was the prominent part in OSCC. Furthermore, our study demonstrated that tumor-derived IL-8 drives ALDH3A1-mediated TANs N2 polarization in the TME through PI3K/AKT/NF-κB pathway in vitro and in vivo.Conclusion: Our results indicate that TANs can serve as a prognostic biomarker and ALDH3A1 could be a promising therapeutic target for regulating TANs N2 polarization in antitumor therapy.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4231-4242"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139472845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linagliptin's impact on lymphatic barrier and lymphangiogenesis in oral cancer with high glucose. 利拉利汀对高糖口腔癌患者淋巴屏障和淋巴管生成的影响

IF 2.9 3区医学

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-02-20 DOI: 10.1111/odi.14893

Hongyu Wang, Xiao She, Qiongdong Xu, Xingyu Zhou, Qinchao Tang, Huakun Wei, Tianjing Huang, Feixin Liang

{"title":"Linagliptin's impact on lymphatic barrier and lymphangiogenesis in oral cancer with high glucose.","authors":"Hongyu Wang, Xiao She, Qiongdong Xu, Xingyu Zhou, Qinchao Tang, Huakun Wei, Tianjing Huang, Feixin Liang","doi":"10.1111/odi.14893","DOIUrl":"10.1111/odi.14893","url":null,"abstract":"Objectives: Uncertainties remain regarding the effect of elevated glucose levels on lymphatic metastasis of cancer cells. Our study elucidated the mechanisms linking high glucose to lymphangiogenesis and lymphatic barrier-related factors and investigated the protective role of linagliptin against lymphatic barrier dysfunction.Materials and methods: A CAL-27-LEC co-culture system was established. Sodium fluorescein permeability assay observed lymphatic endothelial cell permeability. Western blotting and RT-qPCR detected protein and mRNA expression under different conditions, respectively. CCK-8, scratch wound healing, and transwell assays revealed cell migration and proliferation. Tube formation experiment tested capacity for endothelial tube formation. Immunohistochemical staining analyzed tissue sections from 43 oral cancer individuals with/without diabetes.Results: In high-glucose co-culture system, we observed increased lymphatic barrier permeability and decreased expression of ZO-1 and occludin, two tight-junction proteins; conversely, the expression of PAR2, a high permeability-related protein, was increased. Following linagliptin treatment, the expression levels of VEGF-C, VEGFR-3, and PAR2 decreased, while those of ZO-1 and occludin increased. Considerably higher levels of LYVE-1 expression in individuals with diabetes than in those without diabetes.Conclusions: By ameliorating the high glucose-induced disruption of the lymphatic endothelial barrier, linagliptin may reduce lymphangiogenesis and exhibit an inhibitory effect on lymphatic metastasis in oral cancer patients with diabetes.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"4195-4208"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139906255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0