Oral diseases最新文献

Dasatinib and Quercetin Mitigate Age-Related Alveolar Bone Inflammaging and Neutrophil Infiltration.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-06 DOI: 10.1111/odi.15291

Jinru Tang, Jingya Li, Zeyu Hou, Rong He, Bingzhi Li, Jiajing Gong, Yuhang Xie, Wanrong Meng, Yunkun Liu, Takehito Ouchi, Longjiang Li, Bo Li

{"title":"Dasatinib and Quercetin Mitigate Age-Related Alveolar Bone Inflammaging and Neutrophil Infiltration.","authors":"Jinru Tang, Jingya Li, Zeyu Hou, Rong He, Bingzhi Li, Jiajing Gong, Yuhang Xie, Wanrong Meng, Yunkun Liu, Takehito Ouchi, Longjiang Li, Bo Li","doi":"10.1111/odi.15291","DOIUrl":"https://doi.org/10.1111/odi.15291","url":null,"abstract":"Objective: Age-related alveolar bone resorption poses a major dental health challenge, yet its mechanisms and treatments are poorly understood. This study investigates the impact of dasatinib and quercetin (D + Q) treatment on senescent cells (SnCs), senescence-associated secretory phenotype (SASP), and neutrophil infiltration in aged alveolar bone, aiming to develop new strategies for combating age-related bone resorption.Methods: C57BL/6 mice (2 and 18 months) were used to examine alveolar bone resorption, inflammaging, and neutrophil infiltration. Aged mice received D + Q treatment to assess therapeutic effects. Key measurements included cementoenamel junction to the alveolar bone crest (CEJ-ABC) distance, periodontal ligament (PDL) thickness, osteometabolism markers, SnCs accumulation, SASP expression, and neutrophil infiltration.Results: Aged alveolar bone showed increased CEJ-ABC distance, atrophied periodontal ligament, and unbalanced osteometabolism, along with elevated SnCs, SASP, and neutrophils compared to young controls. D + Q treatment improved these conditions by reducing CEJ-ABC distance, enhancing periodontal ligament health, and boosting bone metabolism. It also lowered the expression of SnCs, SASP, and neutrophil markers.Conclusion: D + Q treatment effectively mitigates alveolar bone aging by clearing SnCs, lowering SASP levels, and reducing neutrophil aggregation, presenting a novel approach for age-related bone resorption.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infiltrated Macrophages Aggravate TMJOA Chronic Pain via Piezo2 in IB4⁺-TG Neurons.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-06 DOI: 10.1111/odi.15304

Xueke Jia, Xin Liu, Taomin Zhu, Xiaohan Ma, Ruiming Chen, Huimin Li, Yaping Feng, Liwu Zheng, Yu Liu, Jin Ke

{"title":"Infiltrated Macrophages Aggravate TMJOA Chronic Pain via Piezo2 in IB4+-TG Neurons.","authors":"Xueke Jia, Xin Liu, Taomin Zhu, Xiaohan Ma, Ruiming Chen, Huimin Li, Yaping Feng, Liwu Zheng, Yu Liu, Jin Ke","doi":"10.1111/odi.15304","DOIUrl":"https://doi.org/10.1111/odi.15304","url":null,"abstract":"Objective: Recent research indicates that macrophages in ganglia are linked to chronic pain, with Piezo2 ion channels playing a key role in pain sensation. Our study aims to elucidate the interplay between macrophages and Piezo2 in temporomandibular joint osteoarthritis (TMJOA) chronic pain.Materials and methods: We induced TMJOA chronic pain in rats via articular injection of monosodium iodoacetate (MIA). We then depleted macrophages using clodronate liposomes and overexpressed Piezo2 in trigeminal ganglion (TG) neurons with adeno-associated virus 9 (AAV9)-Piezo2 in TMJOA rats. To explore the connection between macrophages and Piezo2, we employed immunofluorescence, in vitro studies, and the Rat Grimace Scale (RGS) to evaluate pain thresholds in TMJOA rats.Results: A positive correlation was observed between macrophage infiltration and Piezo2 upregulation in TG neurons of TMJOA rats. Depletion of infiltrated macrophages downregulated Piezo2 in TG neurons, while Piezo2 overexpression negated the pain-relieving effects of infiltrated macrophage depletion in TMJOA rats. Macrophages primarily influenced Piezo2 expression in IB4 + - TG neurons of TMJOA chronic pain rats. Ex vivo studies revealed that infiltrated macrophage-derived IL-1β and TNF-α cytokines activate Dil + -TG neurons by upregulating Piezo2 in TMJOA rats.Conclusions: Infiltrated macrophages exacerbate MIA-induced TMJOA chronic pain by upregulating Piezo2 expression in IB4+-TG neurons.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted Therapy for the Management of Oral Potentially Malignant Disorders: A Systematic Review.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-06 DOI: 10.1111/odi.15307

Lauren Frenzel Schuch, Fábio Muradás Girardi, Laura Borges Kirschnick, Aline Lauda Freitas Chaves, Luiz Paulo Kowalski, Ronell Bologna-Molina, William Nassib William Junior, Manoela Domingues Martins, Alan Roger Santos-Silva

{"title":"Targeted Therapy for the Management of Oral Potentially Malignant Disorders: A Systematic Review.","authors":"Lauren Frenzel Schuch, Fábio Muradás Girardi, Laura Borges Kirschnick, Aline Lauda Freitas Chaves, Luiz Paulo Kowalski, Ronell Bologna-Molina, William Nassib William Junior, Manoela Domingues Martins, Alan Roger Santos-Silva","doi":"10.1111/odi.15307","DOIUrl":"https://doi.org/10.1111/odi.15307","url":null,"abstract":"Objective: This study aimed to integrate the available data published in the literature to identify and elucidate the impact of targeted therapy for oral potentially malignant disorders (OPMDs).Material and methods: This systematic review was conducted according to the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) 2020 statement. An electronic search across five databases with no publication date restriction was conducted in January 2023 and subsequently updated in January 2024. Eligibility criteria included studies focusing on targeted therapies. Interventions that either did not align with the specific definition of targeted therapy or failed to meet the criteria for precision-based treatment approaches were excluded from this review.Results: Six clinical trials were identified, all conducted in the United States. The sample size ranged from 12 to 150 subjects. Celecoxib, erlotinib, cetuximab, metformin, and nivolumab were employed as targeted systemic therapies at varying doses. Some patients experienced disease progression with no statistically significant differences in histological improvement rates or size increases. Certain studies reported treatment-related toxicity.Conclusion: The findings of this review do not support the use of targeted therapy in managing OPMDs, highlighting the need for additional clinical trials to further evaluate their efficacy.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Machine Learning in the Diagnosis of Temporomandibular Disorders: An Overview.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-03 DOI: 10.1111/odi.15300

Xiayanran Wu, Yunhao Zheng, Chaolin He, Yiwei Liu, Qian Cheng, Xin Xiong, Jun Wang

{"title":"Application of Machine Learning in the Diagnosis of Temporomandibular Disorders: An Overview.","authors":"Xiayanran Wu, Yunhao Zheng, Chaolin He, Yiwei Liu, Qian Cheng, Xin Xiong, Jun Wang","doi":"10.1111/odi.15300","DOIUrl":"https://doi.org/10.1111/odi.15300","url":null,"abstract":"Objectives: Temporomandibular disorders (TMDs) refer to a group of disorders related to the temporomandibular joint (TMJ), the diagnosis of which is important in dental practice but remains challenging for nonspecialists. With the development of machine learning (ML) methods, ML-based TMDs diagnostic models have shown great potential. The purpose of this review is to summarize the application of ML in TMDs diagnosis, as well as future directions and possible challenges.Methods: PubMed, Google Scholar, and Web of Science databases were searched for electronic literature published up to October 2024, in order to describe the current application of ML in the classification and diagnosis of TMDs.Results: We summarized the application of various ML methods in the diagnosis and classification of different subtypes of TMDs and described the role of different imaging modalities in constructing diagnostic models. Ultimately, we discussed future directions and challenges that ML methods may confront in the application of TMDs diagnosis.Conclusions: The screening and diagnosis models of TMDs based on ML methods hold significant potential for clinical application, but still need to be further verified by a large number of multicenter data and longitudinal studies.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stromal Prognostic Markers for Oral Cancer: An Update.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-03 DOI: 10.1111/odi.15278

Everton Freitas de Morais, Antti Mäkitie, Ricardo D Coletta, Alhadi Almangush

{"title":"Stromal Prognostic Markers for Oral Cancer: An Update.","authors":"Everton Freitas de Morais, Antti Mäkitie, Ricardo D Coletta, Alhadi Almangush","doi":"10.1111/odi.15278","DOIUrl":"https://doi.org/10.1111/odi.15278","url":null,"abstract":"Background: This article aims to provide a broad overview of the oral squamous cell carcinoma (OSCC) tumor stroma, describing and discussing recent advances in the understanding of different stromal elements and their roles in tumor progression. We also describe potential new therapeutic approaches targeting the stroma.Methods: A literature review on the role of stromal biomarkers in OSCC was conducted. A narrative overview of current literature was undertaken to synthesize the contexts with elaboration and summary.Results: The crosstalk between cancer cells and the tumor stroma, a major driver of tumor progression and metastasis, has increasingly been unveiled. This review highlights the tumor-to-stroma ratio (TSR) as a valuable prognostic marker in OSCC, with high stromal content (TSR > 50%) linked to poorer survival. On the other hand, tumor-infiltrating lymphocytes are associated with improved prognosis and longer survival. Furthermore, emerging markers, such as cancer-associated fibroblasts and desmoplastic reactions, play significant roles in promoting tumor invasiveness and resistance to therapy. The review identifies gaps in current evidence and proposes directions for future research to further clarify the prognostic utility of stromal components.Conclusions: Stromal markers provide valuable prognostic insights into OSCC and could enhance clinical decision-making, emphasizing the complexity of OSCC progression.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Characteristics and Prognostic Factors of 199 Salivary Acinic Cell Carcinoma.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-03 DOI: 10.1111/odi.15301

Lijing Zhu, Lisha Sun, Tie-Jun Li

{"title":"Clinical Characteristics and Prognostic Factors of 199 Salivary Acinic Cell Carcinoma.","authors":"Lijing Zhu, Lisha Sun, Tie-Jun Li","doi":"10.1111/odi.15301","DOIUrl":"https://doi.org/10.1111/odi.15301","url":null,"abstract":"Objects: This retrospective study aimed to investigate the clinical characteristics and prognostic features of acinic cell carcinoma (AciCC) of the salivary gland.Methods: One Hundred Ninety-nine cases of AciCC were retrospectively reviewed. Clinical characteristics and outcomes were systematically collected. Recurrence-free survival, metastases-free survival, and overall survival of patients with different clinical variables were compared using the Kaplan-Meier method and Cox univariate regression.Results: A total of 199 patients were included (70 men, 129 women; mean age, 44.57 years). The parotid gland was most frequently affected (75.0%), and the majority of patients were asymptomatic (71.36%). Most patients underwent surgical treatment. Follow-up data were available for 155 patients, with a mean duration of 78.42 months. Local recurrence occurred in 11.6% of these patients, regional metastases in 6.5%, and distant metastases in 5.2%. Of the 10 patients with metastases, 4 died of AciCC. Poorer survival was significantly associated with age > 40 years, perineural invasion, neck dissection, and advanced stage (III/IV). Recurrence and metastases were strongly associated with AciCC-related mortality.Conclusions: Regular follow-up and careful lymph node assessment are critical for patients over 40 years, those with perineural invasion, advanced-stage disease, recurrence, or metastases, as these factors are linked to poorer outcomes in AciCCs.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutational Landscape of Ameloblastoma: Analysis of BRAF and Other Key Mutations in Chinese Patients.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-03 DOI: 10.1111/odi.15286

Zhu You, Zihan Sima, Xiaowen Guo, Xuefen Li, Yanrui Feng, Ning Du, Heyu Zhang, Lisha Sun

{"title":"Mutational Landscape of Ameloblastoma: Analysis of BRAF and Other Key Mutations in Chinese Patients.","authors":"Zhu You, Zihan Sima, Xiaowen Guo, Xuefen Li, Yanrui Feng, Ning Du, Heyu Zhang, Lisha Sun","doi":"10.1111/odi.15286","DOIUrl":"https://doi.org/10.1111/odi.15286","url":null,"abstract":"Objectives: To investigate the prevalence of BRAF, SMO, KRAS, HRAS, NRAS, FGRF2, and CTNNB1 gene mutations in Chinese ameloblastoma (AM) patients and explore their associations with clinical characteristics.Materials and methods: DNA was extracted from 89 formalin-fixed paraffin-embedded AM samples (9 unicystic and 80 conventional). PCR and Sanger sequencing were used to detect mutations, followed by statistical analysis to assess correlations between mutations and clinical variables.Results: BRAF V600E mutations were significantly prevalent, occurring in 92% (59/64) of mandibular AMs compared to 40% (10/25) in maxillary AMs. SMO mutations were found in 20% (5/25) of maxillary and 3.1% (2/64) of mandibular AMs. FGFR2 mutations were detected in six maxillary and two mandibular AMs, while RAS mutations were present in four maxillary and one mandibular AM. No detectable HRAS, NRAS(G12), or CTNNB1 mutations were observed. BRAF mutations showed mutual exclusivity with SMO and FGFR2 mutations.Conclusion: The high prevalence of BRAF V600E mutations, particularly in mandibular AMs, suggests its potential as a diagnostic and therapeutic target. Distinct mutation profiles between maxillary and mandibular AMs indicate molecular diversity. In BRAF-negative cases, alternative oncogenic pathways involving SMO, FGFR2, and RAS may be actionable targets, underscoring the need for personalized treatment approaches.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporomandibular Dysfunction and Sleep Apnea in Dutch Adults With Osteogenesis Imperfecta.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-03 DOI: 10.1111/odi.15280

L Blokland, E de Kuijper-Timmermans, M de Kuijper, K Gooijer, G J Harsevoort, M C Zillikens, N M Appelman-Dijkstra, G J M Janus, S Kalaykova

{"title":"Temporomandibular Dysfunction and Sleep Apnea in Dutch Adults With Osteogenesis Imperfecta.","authors":"L Blokland, E de Kuijper-Timmermans, M de Kuijper, K Gooijer, G J Harsevoort, M C Zillikens, N M Appelman-Dijkstra, G J M Janus, S Kalaykova","doi":"10.1111/odi.15280","DOIUrl":"https://doi.org/10.1111/odi.15280","url":null,"abstract":"Objective: This study investigated self-reported symptoms of temporomandibular disorders, mandibular functional impairment, and obstructive sleep apnea among Dutch adults with Osteogenesis Imperfecta.Materials and methods: A cross-sectional online survey was distributed among Dutch adults with Osteogenesis Imperfecta. Possible risk factors for self-reported temporomandibular disorders (3Q/TMD; DC/TMD), mandibular impairments (Mandibular Function Impairment Questionnaire), and obstructive sleep apnea (STOP-Bang) were explored using univariate and multiple regression analyses.Results: 31% of 155 respondents reported temporomandibular disorder symptoms and 40.0% and 5.2% moderate and severe mandibular function impairment, respectively. Intermediate and severe risk for obstructive sleep apnea were reported by 16.1% and 14.8%, respectively. Female gender and Osteogenesis Imperfecta type III were independently associated with the presence of self-reported temporomandibular disorders. Osteogenesis Imperfecta type III, missing teeth, and self-reported temporomandibular disorders were independently associated with impaired mandibular function. Higher age, male gender, and bisphonate use were independently associated with a higher risk for positive screening for obstructive sleep apnea.Conclusions: A substantial proportion of Dutch adults with Osteogenesis Imperfecta reported symptoms indicative of temporomandibular disorders, mandibular function impairment, and higher risk for obstructive sleep apnea. Especially Osteogenesis Imperfecta type III patients are at risk for self-reported temporomandibular disorders and hampered mandibular function.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Preclinical and Clinical Evidence on the Impact of Phytochemicals in Oral Cancer Metastasis.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-03 DOI: 10.1111/odi.15293

Murugesan Amirtha Varshini, Arikketh Devi

{"title":"Emerging Preclinical and Clinical Evidence on the Impact of Phytochemicals in Oral Cancer Metastasis.","authors":"Murugesan Amirtha Varshini, Arikketh Devi","doi":"10.1111/odi.15293","DOIUrl":"https://doi.org/10.1111/odi.15293","url":null,"abstract":"Objective: This review focuses on the clinical and preclinical studies pertaining to the use of phytochemicals in the prevention of Oral Squamous Cell Carcinoma (OSCC) metastasis.Methods: A thorough overview of the relevant literature was provided by the narrative review approach. The PubMed, Google Scholar, and Scopus databases were used to retrieve publications on the phytochemicals preventing oral cancer metastasis. Clinical trials of phytochemicals on oral cancer patients were retrieved from https://clinicaltrials.gov/.Results: An increasing number of scientific literature provides evidence suggesting that phytochemicals might possess the capacity to impede cancer invasion and metastasis. Plant extracts and phytochemical substances, including genistein, curcumin, lycopene, resveratrol, isothiocyanates, and green tea extract, have shown promise in recent novel research as potential weapons against this cancer.Conclusion: A crucial prognostic factor for oral cancer is distant metastasis (DM). Chemoprevention is a comprehensive concept that encompasses the use of synthetic or natural substances to impede the development or progression of cancer. This current literature review enumerates the data supporting these potential phytochemicals' benefits in OSCC.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Anticoagulant Heparin: An In Vitro Investigation of a Novel Therapeutic Approach for Oral Cancer.

IF 2.9 3区医学

Oral diseases Pub Date : 2025-03-03 DOI: 10.1111/odi.15288

S A Hamza, R Paolini, N M O'Brien-Simpson, W Singleton, R Patini, M McCullough, A Celentano

{"title":"Non-Anticoagulant Heparin: An In Vitro Investigation of a Novel Therapeutic Approach for Oral Cancer.","authors":"S A Hamza, R Paolini, N M O'Brien-Simpson, W Singleton, R Patini, M McCullough, A Celentano","doi":"10.1111/odi.15288","DOIUrl":"https://doi.org/10.1111/odi.15288","url":null,"abstract":"Background: Oral squamous cell carcinoma (OSCC) represents a significant global oral health concern. Non-anticoagulant heparin (NH) emerges as a promising solution considering the enhanced survival observed with anticoagulants in cancer treatment.Methods: We used the MTS assay (0/24/48/72 h), scratch assay (MuviCyte, 0-18 h), invasion Matrigel (24 h), and cytotoxic assay (0-24 h) to assess the in vitro effects of NH and heparin (10, 20, 40, 80 U/mL) on three oral human cell lines (H400/H357/OKF6) as well as their ability to interfere with the chemotherapeutic agents 5FU and cisplatin (1-5 μg/mL).Results: Remarkably, NH not only significantly induced a significant cytotoxic effect on both cancer cell lines at 80 U/mL but also inhibited proliferation at 48/72 h to a comparable extent as heparin. Notably, neither drug exhibited cytotoxic effects on the normal cells. Furthermore, in H400/H357 cells, both heparin and NH significantly inhibit the cell migration and invasion rate. Importantly, the combination of these drugs with commonly used chemotherapeutic agents for OSCC treatment did not compromise their efficacy against the tested cell lines.Conclusion: NH demonstrates promising potential without compromising the efficacy of commonly used chemotherapeutic agents. These results underscore the need for the translation of this research to preclinical animal models.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0