Oral diseases最新文献

Oral Microbiota Dysbiosis Initiated by Chronic Colitis and the Possible Role in Oral Mucosa Changes. 慢性结肠炎引起的口腔微生物群失调及其在口腔黏膜变化中的可能作用。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-19 DOI: 10.1111/odi.15344

Zhuo Wang, Minghui Wei, Jian Wan, Wenfang He, Jiaming Zhou, Yujie Zhang, Yi Liu, Yanxing Liu, Dan Liu, Qinglin Zhu, Xinwen Wang, Kaichun Wu

{"title":"Oral Microbiota Dysbiosis Initiated by Chronic Colitis and the Possible Role in Oral Mucosa Changes.","authors":"Zhuo Wang, Minghui Wei, Jian Wan, Wenfang He, Jiaming Zhou, Yujie Zhang, Yi Liu, Yanxing Liu, Dan Liu, Qinglin Zhu, Xinwen Wang, Kaichun Wu","doi":"10.1111/odi.15344","DOIUrl":"https://doi.org/10.1111/odi.15344","url":null,"abstract":"Objective: To investigate oral microbiota dysbiosis and cytopathological changes in oral mucosa of murine chronic colitis model and the correlation between them.Methods: Dextran sodium sulfate (DSS) induced chronic colitis was established in SPF C57BL/6 male mice, oral microbiome characterization was performed using 16S rRNA gene sequencing, and cytopathological and immunohistochemistry assessment was performed in oral mucosa.Results: When chronic colitis was induced, the overall microbial composition of the oral microbiome was altered with increased abundance in phylum Proteobacteria (82.2%), Actinobacteria (2.6%) and decreased abundance in Firmicutes (12.7%), Bacteroidetes (1.1%). Among the top 10 most abundance genera, Streptococcus was the only genera significantly decreased in colitis mice oral cavity. Meanwhile, oral epithelial hyperplasia was identified in the murine chronic colitis model, and the ki67 expression was significantly upregulated in oral epithelium (p < 0.05). The chronic course of colitis did not lead to obvious inflammatory infiltration in the oral mucosa. Spearman analysis indicated a strong inverse correlation (r = -0.52, p = 0.03) between oral Streptococcus and epithelium thickness.Conclusions: The chronic colitis mice displayed epithelial hyperplasia in the oral mucosa without obvious inflammatory infiltration, which might be associated with oral dysbiosis, especially a decreased abundance of Streptococcus.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remote Consultations for Monitoring Actinic Cheilitis: A Preliminary Non-Randomized Clinical Trial. 远程会诊监测光化性口炎：一项初步的非随机临床试验。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-19 DOI: 10.1111/odi.70010

Mônica Simões Israel, Nathália de Almeida Freire, Bruno Teixeira Gonçalves Rodrigues, Yasmin Muniz Dias, Manoela Domingues Martins, Carlos Augusto Moreira de Sousa, Vinicius Coelho Carrard

{"title":"Remote Consultations for Monitoring Actinic Cheilitis: A Preliminary Non-Randomized Clinical Trial.","authors":"Mônica Simões Israel, Nathália de Almeida Freire, Bruno Teixeira Gonçalves Rodrigues, Yasmin Muniz Dias, Manoela Domingues Martins, Carlos Augusto Moreira de Sousa, Vinicius Coelho Carrard","doi":"10.1111/odi.70010","DOIUrl":"https://doi.org/10.1111/odi.70010","url":null,"abstract":"Objective: To evaluate the feasibility of remote consultation for monitoring actinic cheilitis.Materials and methods: A cross-over, non-randomized clinical trial comparing remote and in-person consultations for patients with actinic cheilitis. During the remote consultations, patients were interviewed and submitted clinical photos taken with smartphones. Local signs and symptoms were recorded, and examiners assessed whether a biopsy was indicated. Photos sent by patients were compared with those taken during the in-person visits.Results: The study included 36 patients. The most frequent clinical presentation was plaques with erosive areas. Agreement between remote and in-person evaluations regarding clinical alterations was 97% or higher. In 11% of cases (n = 4), both examiners recommended biopsy. In only 2 cases (5.6%), the remote examiner considered an in-person consultation necessary. Photos taken by patients were rated as adequate in 88.6% of cases, compared to 97.1% for professional photos, with no statistically significant difference (p = 0.16).Conclusion: Teledentistry showed promising results for the follow-up of actinic cheilitis and may help reduce healthcare costs. However, due to its potentially malignant nature, initial assessment must be performed by an oral medicine specialist to ensure appropriate diagnosis and management.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel COL12A1 Mutation Causes Oral Tissue Abnormalities by Regulating Gingival Fibroblast Function. 一种新的COL12A1突变通过调节牙龈成纤维细胞功能导致口腔组织异常。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-18 DOI: 10.1111/odi.70000

Shi Yu, Yuanyuan Wang, Xiaojing Yuan, Yi Yang, Xin Wang, Man Qin

{"title":"A Novel COL12A1 Mutation Causes Oral Tissue Abnormalities by Regulating Gingival Fibroblast Function.","authors":"Shi Yu, Yuanyuan Wang, Xiaojing Yuan, Yi Yang, Xin Wang, Man Qin","doi":"10.1111/odi.70000","DOIUrl":"https://doi.org/10.1111/odi.70000","url":null,"abstract":"Objective: This study aimed to identify a novel COL12A1 mutation in a patient with Ullrich congenital muscular dystrophy-2 (UCMD2) presenting with gingival hyperplasia and skeletal anomalies and to characterize its functional impact on gingival fibroblasts (GFs) behavior.Methods: Whole-exome sequencing identified COL12A1 mutations in a consanguineous family. GFs isolated from the patient and healthy controls underwent functional assays to assess proliferation, apoptosis, and osteogenic differentiation. Lentiviral COL12A1 knockdown in GFs validated phenotypic changes. RNA sequencing elucidated altered molecular pathways.Results: A homozygous COL12A1 frameshift mutation (NM_004370: c.6747del, p.Thr2249Thrfs*44) caused collagen XII deficiency. Patient-derived GFs exhibited hyperproliferation (elevated cyclin D1/PCNA, S-phase accumulation), reduced apoptosis (increased Bcl2/Bax ratio), and impaired osteogenic differentiation (downregulated RUNX2, OCN, OPN; reduced mineralization). COL12A1 knockdown recapitulated these defects. Transcriptomics revealed upregulated interferon-alpha/beta response and apoptotic signaling pathways, alongside downregulated extracellular matrix (ECM) organization, cell adhesion, and skeletal development genes in COL12A1-deficient GFs.Conclusion: COL12A1 deficiency disrupts gingival homeostasis by driving fibroblast hyperproliferation, inhibiting fibroblast apoptosis, and suppressing osteogenic differentiation via dysregulated ECM remodeling. These findings establish collagen XII as a critical regulator of neural crest-derived oral connective tissues, providing mechanistic insights into gingival hyperplasia and skeletal anomalies in COL12A1-related disorders.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Experimental Periodontitis and Periodontal Treatment on Alzheimer-Like Pathology in Rats. 实验性牙周炎及牙周治疗对大鼠阿尔茨海默样病理的影响。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-16 DOI: 10.1111/odi.70001

Sedanur Yavuz, Birsen Elibol, Esra Demir, Beyza Kinsiz, Ozlem Toluk, Sadiye Gunpinar

{"title":"Effects of Experimental Periodontitis and Periodontal Treatment on Alzheimer-Like Pathology in Rats.","authors":"Sedanur Yavuz, Birsen Elibol, Esra Demir, Beyza Kinsiz, Ozlem Toluk, Sadiye Gunpinar","doi":"10.1111/odi.70001","DOIUrl":"https://doi.org/10.1111/odi.70001","url":null,"abstract":"Objective: This study investigated the effects of periodontitis (P) and non-surgical periodontal therapy (NSPT) on behavior, neurodegeneration, and neuroinflammation in rats with Alzheimer's disease (AD)-like pathology.Methods: AD-like pathology was induced in rats (n = 28) using STZ neurodegeneration model. Periodontitis was experimentally induced (n = 32), and half of which received NSPT with Chlorhexidine (CHX) gel. Behavioral assessment included the passive avoidance task (PA) and Morris water maze (MWM). Levels of NLRP3, phosphorylated tau (p-tau), and tau in the hippocampus, cerebrospinal fluid (CSF), and serum were measured by ELISA, while BACE1, IL1β, iNOS, and NF-κβ proteins were assessed by Western blotting.Results: Rats in the AD and AD + P groups performed worse in behavioral tests compared to controls (p < 0.05), whereas the NSPT group showed similar performance to controls (p > 0.05). CSF p-tau levels were comparable between AD and AD + P groups, but the hippocampal p-tau/tau ratio was significantly higher in the AD + P group (p < 0.05). BACE1 levels were similar in P and AD groups. NLRP3 and iNOS levels did not show significant differences across groups. Notably, the NSPT group exhibited reduced NF-κβ levels (p < 0.05).Conclusions: Periodontitis may exacerbate AD-like molecular pathology, particularly by promoting tau hyperphosphorylation, while NSPT appears to mitigate disease progression and improve behavioral outcomes.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrate Prevents Sjögren's Disease by Modulating T Helper Cells via NF-κB Pathway Suppression. 硝酸盐通过抑制NF-κB通路调节T辅助细胞预防Sjögren疾病。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-16 DOI: 10.1111/odi.70004

Conglin Du, Zi Yang, Yang Yang, Chunmei Zhang, Hideaki Kagami, Xianqi Li, Liang Hu, Junji Xu, Jian Zhou

{"title":"Nitrate Prevents Sjögren's Disease by Modulating T Helper Cells via NF-κB Pathway Suppression.","authors":"Conglin Du, Zi Yang, Yang Yang, Chunmei Zhang, Hideaki Kagami, Xianqi Li, Liang Hu, Junji Xu, Jian Zhou","doi":"10.1111/odi.70004","DOIUrl":"https://doi.org/10.1111/odi.70004","url":null,"abstract":"Objective: Sjögren's disease (SjD) is a chronic autoimmune disease characterized by abnormal T helper (Th) cell distribution in the salivary glands (SGs). Although nitrate can regulate immune responses and preserve SGs function, its preventive effects on SjD remain unexplored.Methods: Nonobese diabetic (NOD)/Ltj mice were used as a secondary Sjögren's disease (sSjD) animal model. From 6 to 16 weeks of age, the mice received either sodium nitrate (2 mM) in drinking water (SjD + Nit group) or sodium chloride (SjD + NaCl group). Salivary flow rates were recorded biweekly, and submandibular glands were collected at 16 weeks for histological analysis. Th subsets ratios were determined using flow cytometry, and mRNA sequencing was used to explore nitrate's preventive mechanism.Results: Nitrate pretreatment preserved salivary function and reduced lymphocyte infiltration. T cell related genes were downregulated, while salivary function genes were upregulated. Differentiation pathways for Th cell subsets were downregulated in the SjD + Nit group, and nitrate modulated the abnormal balance of Th cells, regulatory T cells (Tregs) in the SGs, and peripheral blood, suppressing the NF-κb pathway of sSjD.Conclusions: Preventive nitrate administration may preserve SG function, rebalance Th cells, and suppress NF-κB pathway, offering a potential treatment for SjD.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Validation of ADAM9 as a Prognostic Biomarker in Oral Cancer. ADAM9作为口腔癌预后生物标志物的临床验证

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-16 DOI: 10.1111/odi.15383

Jeng-Wei Lu, Pei-Chen Shih, Show-Mei Chuang, Wan-Jung Tu, Ming-Heng Tsai, Yun-Jung Tsai, Shu-Hui Lin

{"title":"Clinical Validation of ADAM9 as a Prognostic Biomarker in Oral Cancer.","authors":"Jeng-Wei Lu, Pei-Chen Shih, Show-Mei Chuang, Wan-Jung Tu, Ming-Heng Tsai, Yun-Jung Tsai, Shu-Hui Lin","doi":"10.1111/odi.15383","DOIUrl":"https://doi.org/10.1111/odi.15383","url":null,"abstract":"Background: Oral cancer has a high incidence in Taiwan, and identifying prognostic biomarkers is crucial. This study investigated the role of a disintegrin and metalloprotease 9 (ADAM9) in oral cancer progression and outcomes.Methods: This study investigated ADAM9 protein expression in 353 oral cancer tissue specimens through immunohistochemical (IHC) analysis.Results: The analysis revealed that, among the 353 patients, 21 (6%) exhibited low ADAM9 expression, while the remaining 332 patients (94%) showed high ADAM9 expression, which correlated with advanced T status, poor overall survival, and unfavorable prognosis. Kaplan-Meier analysis confirmed that higher ADAM9 expression predicted significantly worse survival. Univariate and multivariate analyses identified ADAM9, histological grade, and AJCC stage as independent prognostic factors. Functionally, ADAM9 silencing in SAS and OC2 cells inhibited invasion and migration, downregulating matrix metalloproteinase 9 (MMP9) and matrix metalloproteinase 14 (MMP14). siRNA-mediated ADAM9 knockdown also reduced cell viability and migration, as confirmed by cell counting kit-8 and transwell assays. The Cancer Genome Atlas (TCGA) analysis further revealed a positive correlation between ADAM9 mRNA levels and matrix metalloproteinase 2 (MMP2) or MMP14 expression in oral cancer patients.Conclusions: This study identifies ADAM9 as a key driver of oral cancer in a Taiwanese cohort and highlights its diagnostic and therapeutic potential.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Oral Melanocytic Nevi in Sun-Exposed and Sun-Protected Regions. 日晒和防晒地区口腔黑素细胞痣的特征。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-12 DOI: 10.1111/odi.15404

Thalita Soares Tavares, Adriana Aparecida Silva da da Costa, Marina Gonçalves Diniz, Daniela Pereira Meirelles, Sandra Beatriz Chaves Tarquínio, Ana Carolina Uchoa Vasconcelos, Aline Cristina Batista Rodrigues Johann, Ricardo Santiago Gomez, de Aguiar, Patrícia Carlos Caldeira

{"title":"Characterization of Oral Melanocytic Nevi in Sun-Exposed and Sun-Protected Regions.","authors":"Thalita Soares Tavares, Adriana Aparecida Silva da da Costa, Marina Gonçalves Diniz, Daniela Pereira Meirelles, Sandra Beatriz Chaves Tarquínio, Ana Carolina Uchoa Vasconcelos, Aline Cristina Batista Rodrigues Johann, Ricardo Santiago Gomez, de Aguiar, Patrícia Carlos Caldeira","doi":"10.1111/odi.15404","DOIUrl":"https://doi.org/10.1111/odi.15404","url":null,"abstract":"Objective: Oral melanocytic nevi (OMN) are histologically similar to skin nevi; however, they are much rarer and not always related to sun exposure. This study aimed to characterize the histopathological, immunohistochemical, and molecular features of OMN in sun-exposed (vermilion lip) and sun-protected (intraoral) regions.Method: Cross-sectional study on 14 intraoral and 20 vermilion lip OMN. Hematoxylin-eosin, immunohistochemistry for TRP2 and MiTF, and RT-qPCR for TRP2 and MiTF were used to assess histopathology, protein, and mRNA expression, respectively. Statistical analysis was performed.Results: Intramucous and blue nevi are the most frequent histological subtypes. Intraoral lesions predominantly affect Brown or Black individuals (64.3%), presenting mostly as macules. Vermilion lip nevi affect White individuals (75%), with elevated lesions (30%). Histologically, intraoral nevi show asymmetric, lack theques formation and floret cells, and present no mitotic activity or pleomorphism, while vermilion nevi display pleomorphism, symmetry, theques formation, floret cells, and solar elastosis. TRP2 and MiTF protein and mRNA expression were upregulated in both groups (p < 0.05), with no differences between them (p > 0.05).Conclusions: Intraoral and vermilion lip nevi have distinct histopathological features, but similar TRP2 and MiTF protein and mRNA expression. This characterization may aid the histopathological diagnosis of OMN and distinction from other oral pigmentations.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Applications and Mechanisms of Klotho in Periodontitis Diagnosis and Therapy. Klotho在牙周炎诊治中的临床应用及作用机制。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-12 DOI: 10.1111/odi.15402

Chengyu Wan, Yalan Yang, Bozhao Wang, Jian Li

{"title":"Clinical Applications and Mechanisms of Klotho in Periodontitis Diagnosis and Therapy.","authors":"Chengyu Wan, Yalan Yang, Bozhao Wang, Jian Li","doi":"10.1111/odi.15402","DOIUrl":"https://doi.org/10.1111/odi.15402","url":null,"abstract":"Objectives: Periodontitis is a prevalent chronic inflammatory condition affecting the periodontal supporting tissue, and Klotho has anti-inflammatory and antioxidant effects. In recent years, some emerging evidence has revealed a link between Klotho and periodontitis. This review aims to elucidate the effects of Klotho in periodontitis and summarize the methods to increase Klotho levels for clinical treatment.Subjects and methods: We conducted a systematic search of PubMed, Web of Science, and Embase databases to review the current research status of Klotho in relation to periodontitis and its roles in periodontal disease mechanisms.Results: Klotho demonstrates anti-inflammatory, antioxidant, autophagy-enhancing, and alveolar bone regeneration-promoting effects in periodontitis. Feasible strategies to elevate Klotho levels in humans include exogenous supplementation of Klotho and modulation of its upstream regulatory pathways.Conclusion: Klotho and its associated enhancers show significant therapeutic potential for the management of periodontitis; however, the underlying mechanisms and more clinical studies are still lacking, and further research is needed.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KL-12 Peptide Targets Peri-Implantitis via Dual Antibacterial and Immunomodulatory Effects. KL-12肽通过双重抗菌和免疫调节作用靶向种植体周围炎。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-12 DOI: 10.1111/odi.15401

Jiahao Qin, Shuwei Yang, Weixu Meng, Zanxun Zhou, Zihui Yu, Xi Zhang

{"title":"KL-12 Peptide Targets Peri-Implantitis via Dual Antibacterial and Immunomodulatory Effects.","authors":"Jiahao Qin, Shuwei Yang, Weixu Meng, Zanxun Zhou, Zihui Yu, Xi Zhang","doi":"10.1111/odi.15401","DOIUrl":"https://doi.org/10.1111/odi.15401","url":null,"abstract":"Aims: Peri-implantitis is a common dental complication characterized by inflammation of peri-implant tissues and progressive bone loss. With the rise of antibiotic resistance, this study investigates the potential of antimicrobial peptides (AMPs), specifically KL-12, a novel peptide derived from cecropin B, as an alternative therapeutic strategy against Porphyromonas gingivalis, a key pathogen in peri-implantitis.Methods: We evaluated the structural stability, antibacterial efficacy, and cytotoxicity of KL-12. Antibacterial activity was measured against P. gingivalis, while the peptide's mode of action was explored concerning its effect on the gingipain proteases essential for the bacterium's nutrient acquisition. Additionally, we assessed KL-12's impact on macrophage polarization and anti-inflammatory responses.Results: The findings revealed that KL-12 exhibited significant antibacterial activity against P. gingivalis and demonstrated potential as a specific inhibitor of gingipains. It was observed that KL-12 disrupts bacterial viability through mechanisms involving deformation and nutrient deprivation, rather than traditional pore formation. KL-12 effectively modulated macrophage polarization, promoting M2 differentiation and enhancing anti-inflammatory responses.Conclusion: These results suggest that KL-12 functions as a dual-action therapeutic agent, targeting both bacterial pathogens and inflammatory processes in the management of peri-implantitis. Future studies will aim to clarify the molecular mechanisms underlying KL-12's antibacterial and immunomodulatory effects.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell RNA Sequencing Study on Two Synovial Derived Tumors in the Temporomandibular Joint. 两种颞下颌关节滑膜衍生肿瘤的单细胞RNA测序研究。

IF 2.9 3区医学

Oral diseases Pub Date : 2025-06-11 DOI: 10.1111/odi.70003

Weihua Han, Tiansong Xu, Zonghan He, Haiyan Luo, Chuanbin Guo, Juanhong Meng

{"title":"Single-Cell RNA Sequencing Study on Two Synovial Derived Tumors in the Temporomandibular Joint.","authors":"Weihua Han, Tiansong Xu, Zonghan He, Haiyan Luo, Chuanbin Guo, Juanhong Meng","doi":"10.1111/odi.70003","DOIUrl":"https://doi.org/10.1111/odi.70003","url":null,"abstract":"Objective: To investigate the cellular composition and differentiation trajectory of synovial chondromatosis (SC) and diffuse tenosynovial giant cell tumor (D-TSGCT) in the temporomandibular joint (TMJ).Materials and methods: Single-cell RNA sequencing was applied to analyze the cellular composition, differentiation trajectory, and intercellular communication of SC and D-TSGCT. Cell culture and morphological experiments were applied to validate the chondrogenic differentiation potential of fibroblast-like synoviocytes (FLS) in the initial stage of SC and to explore the expression and regulation of differential genes during chondrogenic differentiation.Results: SC was mainly composed of FLS and chondrocytes, while D-TSGCT had a relatively complex cellular composition. Genetically, chondrogenesis-related genes such as COMP and SMAD2 were upregulated in the early stage of SC. TGFβ pathway and collagen catabolic process were subsequently activated, ultimately leading to cartilage formation. CSF1 and CD68 were overexpressed at the forming stage of D-TSGCT, and the macrophage pathway was enriched in the later phase. Based on experimental verification, COMP could induce chondrogenic differentiation of FLS by activating TGFβ/SMAD signal pathways in vitro.Conclusions: FLS are the common starting point of synovial-derived tumors, but differentiate toward different endpoints in the SC and D-TSGCT. COMP/TGFβ/SMAD signaling pathways can promote chondrogenic differentiation of synovium in SC.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0