Lrp5 A241T High-Bone-Mass Mutation Enhances Implant Osseointegration: An Experimental Murine Study.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-09-30 DOI:10.1111/odi.70102

Danhong Zhou, Xueting Wang, Hui Zhang, Qifeng Jiang, Qinmeng Zhang, Xiaoyan Miao, Ying Wang, Guoli Yang, Zhiwei Jiang

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引用次数: 0

Abstract

Objectives: This study aimed to assess the effect of the low-density lipoprotein receptor-related protein 5 (Lrp5) A241T gain-of-function mutation, which induces a high-bone-mass phenotype, on implant osseointegration in a murine model.

Methods: Heterozygous Lrp5 A241T mutant (Lrp5^A/+) and wild-type (WT) littermate male mice on a C57BL/6J background were used. Micro-computed tomography (micro-CT) was performed to assess tibial trabecular bone architecture. Sandblasted and acid-etched (SLA) titanium implants were surgically inserted into the proximal tibiae, and samples were harvested after 4 weeks for micro-CT and histological analysis. Primary osteoblasts were seeded onto SLA titanium sheets and induced for osteogenesis for 7 days. Osteogenic marker expression was assessed by quantitative real-time polymerase chain reaction and western blotting.

Results: The Lrp5^A/+ group exhibited significantly improved trabecular bone architecture compared to WT controls. At 4 weeks post-implantation, micro-CT revealed greater peri-implant bone formation in the Lrp5^A/+ group, while hard tissue sections showed increased bone area and bone-implant contact. HE and Masson staining further confirmed greater new bone formation. After 7 days of in vitro osteogenic induction, Lrp5, Runx2, and Sp7 were significantly upregulated in the Lrp5^A/+ group.

Conclusions: The Lrp5 A241T mutation significantly enhanced implant osseointegration in a transgenic murine model.

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Lrp5 A241T高骨量突变促进种植体骨整合：一项实验小鼠研究

目的：本研究旨在评估诱导高骨量表型的低密度脂蛋白受体相关蛋白5 (Lrp5) A241T功能获得突变对小鼠种植体骨整合的影响。方法：采用杂合子Lrp5 A241T突变体（Lrp5A/+）和C57BL/6J基因背景的野生型雄性小鼠。微计算机断层扫描（micro-CT）评估胫骨小梁骨结构。手术将喷砂酸蚀（SLA）钛植入胫骨近端，4周后采集标本进行显微ct和组织学分析。将初代成骨细胞植入SLA钛板，诱导成骨7 d。采用实时定量聚合酶链反应和western blotting检测成骨标志物的表达。结果：与WT对照组相比，Lrp5A/+组表现出明显改善的骨小梁结构。植入后4周，显微ct显示Lrp5A/+组种植体周围骨形成更大，硬组织切片显示骨面积和骨与种植体接触增加。HE和Masson染色进一步证实了更多的新骨形成。体外成骨诱导7 d后，Lrp5A/+组Lrp5、Runx2、Sp7显著上调。结论：Lrp5 A241T突变显著增强了转基因小鼠种植体骨整合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.