Organogenesis最新文献

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Embryonic skin development and repair. 胚胎皮肤发育和修复。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-02-15 DOI: 10.1080/15476278.2017.1421882
Michael S Hu, Mimi R Borrelli, Wan Xing Hong, Samir Malhotra, Alexander T M Cheung, Ryan C Ransom, Robert C Rennert, Shane D Morrison, H Peter Lorenz, Michael T Longaker
{"title":"Embryonic skin development and repair.","authors":"Michael S Hu, Mimi R Borrelli, Wan Xing Hong, Samir Malhotra, Alexander T M Cheung, Ryan C Ransom, Robert C Rennert, Shane D Morrison, H Peter Lorenz, Michael T Longaker","doi":"10.1080/15476278.2017.1421882","DOIUrl":"10.1080/15476278.2017.1421882","url":null,"abstract":"<p><p>Fetal cutaneous wounds have the unique ability to completely regenerate wounded skin and heal without scarring. However, adult cutaneous wounds heal via a fibroproliferative response which results in the formation of a scar. Understanding the mechanism(s) of scarless wound healing leads to enormous clinical potential in facilitating an environment conducive to scarless healing in adult cutaneous wounds. This article reviews the embryonic development of the skin and outlines the structural and functional differences in adult and fetal wound healing phenotypes. A review of current developments made towards applying this clinical knowledge to promote scarless healing in adult wounds is addressed.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 1","pages":"46-63"},"PeriodicalIF":2.3,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150059/pdf/kogg-14-01-1421882.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35809515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erratum. 勘误表。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-02 Epub Date: 2017-12-06 DOI: 10.1080/15476278.2017.1407509
{"title":"Erratum.","authors":"","doi":"10.1080/15476278.2017.1407509","DOIUrl":"https://doi.org/10.1080/15476278.2017.1407509","url":null,"abstract":"Correspondence to: Yumei Zhao, PhD, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Pediatric Dentistry, School of Stomatology, Tongji University, 339 Middle Yanchang Road, Shanghai, 200072, China. Email: yumeizhao@tongji.edu.cn; Shouliang Zhao, PhD, Department of Stomatology, Huashan Hospital, Fudan University, 12 Urumqi Road, Shanghai, 200040, China. Email: slzhao@fudan.edu.cn; Shangfeng Liu, PhD, Department of Stomatology, Huashan Hospital, Fudan University, 12 Urumqi Road, Shanghai, 200040, China. Email: shangfengliufudan@163.com.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 1","pages":"64"},"PeriodicalIF":2.3,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2017.1407509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35583610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells. 猪脱细胞真皮基质处理对伤口愈合和瘢痕形成的影响:表皮干细胞中Jag1表达的作用
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-04-27 DOI: 10.1080/15476278.2018.1436023
Xiao-Dong Chen, Shu-Bin Ruan, Ze-Peng Lin, Ziheng Zhou, Feng-Gang Zhang, Rong-Hua Yang, Ju-Lin Xie
{"title":"Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells.","authors":"Xiao-Dong Chen,&nbsp;Shu-Bin Ruan,&nbsp;Ze-Peng Lin,&nbsp;Ziheng Zhou,&nbsp;Feng-Gang Zhang,&nbsp;Rong-Hua Yang,&nbsp;Ju-Lin Xie","doi":"10.1080/15476278.2018.1436023","DOIUrl":"https://doi.org/10.1080/15476278.2018.1436023","url":null,"abstract":"<p><p>Skin wound healing involves Notch/Jagged1 signaling. However, little is known how Jag1 expression level in epidermal stem cells (ESCs) contributes to wound healing and scar formation. We applied multiple cellular and molecular techniques to examine how Jag1 expression in ESCs modulates ESCs differentiation to myofibroblasts (MFB) in vitro, interpret how Jag1 expression in ESCs is involved in wound healing and scar formation in mice, and evaluate the effects of porcine acellular dermal matrix (ADM) treatment on wound healing and scar formation. We found that Jag1, Notch1 and Hes1 expression was up-regulated in the wound tissue during the period of wound healing. Furthermore, Jag1 expression level in the ESCs was positively associated with the level of differentiation to MFB. ESC-specific knockout of Jag1 delayed wound healing and promoted scar formation in vivo. In addition, we reported that porcine ADM treatment after skin incision could accelerate wound closure and reduce scar formation in vivo. This effect was associated with decreased expression of MFB markers, including α-SMA Col-1 and Col-III in wound tissues. Finally, we confirmed that porcine ADM treatment could increase Jag1, Notch1 and Hesl expression in wound tissues. Taken together, our results suggested that ESC-specific Jag1 expression levels are critical for wound healing and scar formation, and porcine ADM treatment would be beneficial in promoting wound healing and preventing scar formation by enhancing Notch/Jagged1 signaling pathway in ESCs.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 1","pages":"25-35"},"PeriodicalIF":2.3,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1436023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35810874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures. 3D打印结构中原发性肝细胞维持的肝脏特异性功能延长。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-02-01 DOI: 10.1080/15476278.2018.1423931
Yohan Kim, Kyojin Kang, Sangtae Yoon, Ji Sook Kim, Su A Park, Wan Doo Kim, Seung Bum Lee, Ki-Young Ryu, Jaemin Jeong, Dongho Choi
{"title":"Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures.","authors":"Yohan Kim,&nbsp;Kyojin Kang,&nbsp;Sangtae Yoon,&nbsp;Ji Sook Kim,&nbsp;Su A Park,&nbsp;Wan Doo Kim,&nbsp;Seung Bum Lee,&nbsp;Ki-Young Ryu,&nbsp;Jaemin Jeong,&nbsp;Dongho Choi","doi":"10.1080/15476278.2018.1423931","DOIUrl":"https://doi.org/10.1080/15476278.2018.1423931","url":null,"abstract":"<p><p>Isolated primary hepatocytes from the liver are very similar to in vivo native liver hepatocytes, but they have the disadvantage of a limited lifespan in 2D culture. Although a sandwich culture and 3D organoids with mesenchymal stem cells (MSCs) as an attractive assistant cell source to extend lifespan can be used, it cannot fully reproduce the in vivo architecture. Moreover, long-term 3D culture leads to cell death because of hypoxic stress. Therefore, to overcome the drawback of 2D and 3D organoids, we try to use a 3D printing technique using alginate hydrogels with primary hepatocytes and MSCs. The viability of isolated hepatocytes was more than 90%, and the cells remained alive for 7 days without morphological changes in the 3D hepatic architecture with MSCs. Compared to a 2D system, the expression level of functional hepatic genes and proteins was higher for up to 7 days in the 3D hepatic architecture. These results suggest that both the 3D bio-printing technique and paracrine molecules secreted by MSCs supported long-term culture of hepatocytes without morphological changes. Thus, this technique allows for widespread expansion of cells while forming multicellular aggregates, may be applied to drug screening and could be an efficient method for developing an artificial liver.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 1","pages":"1-12"},"PeriodicalIF":2.3,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1423931","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35759824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Sodium hydroxide based non-detergent decellularizing solution for rat lung. 大鼠肺用氢氧化钠非去污剂脱细胞液。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-11 DOI: 10.1080/15476278.2018.1462432
Hideyori Sengyoku, Tomoshi Tsuchiya, Tomohiro Obata, Ryoichiro Doi, Yasumasa Hashimoto, Mitsutoshi Ishii, Hiromi Sakai, Naoto Matsuo, Daisuke Taniguchi, Takashi Suematsu, Murray Lawn, Keitaro Matsumoto, Takuro Miyazaki, Takeshi Nagayasu
{"title":"Sodium hydroxide based non-detergent decellularizing solution for rat lung.","authors":"Hideyori Sengyoku,&nbsp;Tomoshi Tsuchiya,&nbsp;Tomohiro Obata,&nbsp;Ryoichiro Doi,&nbsp;Yasumasa Hashimoto,&nbsp;Mitsutoshi Ishii,&nbsp;Hiromi Sakai,&nbsp;Naoto Matsuo,&nbsp;Daisuke Taniguchi,&nbsp;Takashi Suematsu,&nbsp;Murray Lawn,&nbsp;Keitaro Matsumoto,&nbsp;Takuro Miyazaki,&nbsp;Takeshi Nagayasu","doi":"10.1080/15476278.2018.1462432","DOIUrl":"https://doi.org/10.1080/15476278.2018.1462432","url":null,"abstract":"<p><p>Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches are possible. Here we used high alkaline (pH12) sodium hydroxide (NaOH)-PBS solution without detergents for lung decellularization and compared the efficacy on DNA elimination and ECM preservation with detergent based decellularization solutions CHAPS and SDS. Immunohistochemical image analysis showed that cell components were removed by NaOH solution as well as other detergents. A Collagen and GAG assay showed that the collagen reduction of the NaOH group was comparable to that of the CHAPS and SDS groups. However, DNA reduction was more significant in the NaOH group than in other groups (p < 0.0001). The recellularization of HUVEC revealed cell attachment was not inferior to that of the SDS group. Ex vivo functional analysis showed 100% oxygen ventilation increased oxygen partial pressure as artificial hemoglobin vesicle-PBS solution passed through regenerated lungs in the SDS or NaOH group. It was concluded that the NaOH-PBS based decellularization solution was comparable to ordinal decellularizaton solutions and competitive in cost effectiveness and residues in the decellularized scaffold negligible, thus providing another potential option to detergent for future clinical usage.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 2","pages":"94-106"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1462432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36211225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Bioinspired liver scaffold design criteria. 仿生肝支架设计标准。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-29 DOI: 10.1080/15476278.2018.1505137
Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia
{"title":"Bioinspired liver scaffold design criteria.","authors":"Giorgio Mattei,&nbsp;Chiara Magliaro,&nbsp;Andrea Pirone,&nbsp;Arti Ahluwalia","doi":"10.1080/15476278.2018.1505137","DOIUrl":"10.1080/15476278.2018.1505137","url":null,"abstract":"<p><p>Maintaining hepatic functional characteristics in-vitro is considered one of the main challenges in engineering liver tissue. As hepatocytes cultured ex-vivo are deprived of their native extracellular matrix (ECM) milieu, developing scaffolds that mimic the biomechanical and physicochemical properties of the native ECM is thought to be a promising approach for successful tissue engineering and regenerative medicine applications. On the basis that the decellularized liver matrix represents the ideal design template for engineering bioinspired hepatic scaffolds, to derive quantitative descriptors of liver ECM architecture, we characterised decellularised liver matrices in terms of their biochemical, viscoelastic and structural features along with porosity, permeability and wettability. Together, these data provide a unique set of quantitative design criteria which can be used to generate guidelines for fabricating biomaterial scaffolds for liver tissue engineering. As proof-of-concept, we investigated hepatic cell response to substrate viscoelasticity. On collagen hydrogels mimicking decellularised liver mechanics, cells showed superior morphology, higher viability and albumin secretion than on stiffer and less viscous substrates. Although scaffold properties are generally inspired by those of native tissues, our results indicate significant differences between the mechano-structural characteristics of untreated and decellularised hepatic tissue. Therefore, we suggest that design rules - such as mechanical properties and swelling behaviour - for engineering biomimetic scaffolds be re-examined through further studies on substrates matching the features of decellularized liver matrices.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 3","pages":"129-146"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1505137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36441030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection. 一种用于高灵敏度细胞分泌组检测的微流控竞争性免疫聚集试验。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-08 DOI: 10.1080/15476278.2018.1461306
Fan Liu, Pawan Kc, Liwei Ni, Ge Zhang, Jiang Zhe
{"title":"A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection.","authors":"Fan Liu,&nbsp;Pawan Kc,&nbsp;Liwei Ni,&nbsp;Ge Zhang,&nbsp;Jiang Zhe","doi":"10.1080/15476278.2018.1461306","DOIUrl":"https://doi.org/10.1080/15476278.2018.1461306","url":null,"abstract":"<p><p>We report a high-sensitivity cell secretome detection method using competitive immuno-aggregation and a micro-Coulter counter. A target cell secretome protein competes with anti-biotin-coated microparticles (MPs) to bind with a biotinylated antibody (Ab), causing decreased aggregation of the functionalized MPs and formation of a mixture of MPs and aggregates. In comparison, without the target cell secretome protein, more microparticles are functionalized, and more aggregates are formed. Thus, a decrease in the average volume of functionalized microparticles/aggregates indicates an increase in cell secretome concentration. This volume change is measured by the micro-Coulter counter, which is used to quantitatively estimate the cell secretome concentration. Vascular endothelial growth factor (VEGF), one of the key cell secretome proteins that regulate angiogenesis and vascular permeabilization, was used as the target protein to demonstrate the sensing principle. A standard calibration curve was generated by testing samples with various VEGF concentrations. A detection range from 0.01 ng/mL to 100.00 ng/mL was achieved. We further demonstrated the quantification of VEGF concentration in exogenous samples collected from the secretome of human mesenchymal stem cells (hMSCs) at different incubation times. The results from the assay agree well with the results of a parallel enzyme-linked immunoabsorbent assay (ELISA) test, indicating the specificity and reliability of the competitive immuno-aggregation assay. With its simple structure and easy sample preparation, this assay not only enables high sensitivity detection of VEGF but also can be readily extended to other types of cell secretome analysis as long as the specific Ab is known.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 2","pages":"67-81"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1461306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36206372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution. 无血清和无白蛋白的内皮单层低温保存新方法。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-06 DOI: 10.1080/15476278.2018.1501136
Gesine Pless-Petig, Sven Knoop, Ursula Rauen
{"title":"Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution.","authors":"Gesine Pless-Petig,&nbsp;Sven Knoop,&nbsp;Ursula Rauen","doi":"10.1080/15476278.2018.1501136","DOIUrl":"https://doi.org/10.1080/15476278.2018.1501136","url":null,"abstract":"<p><p>Cryopreservation is the only long-term storage option for the storage of vessels and vascular constructs. However, endothelial barrier function is almost completely lost after cryopreservation in most established cryopreservation solutions. We here aimed to improve endothelial function after cryopreservation using the 2D-model of porcine aortic endothelial cell monolayers. The monolayers were cryopreserved in cell culture medium or cold storage solutions based on the 4°C vascular preservation solution TiProtec<sup>®</sup>, all supplemented with 10% DMSO, using different temperature gradients. After short-term storage at -80°C, monolayers were rapidly thawed and re-cultured in cell culture medium. Thawing after cryopreservation in cell culture medium caused both immediate and delayed cell death, resulting in 11 ± 5% living cells after 24 h of re-culture. After cryopreservation in TiProtec and chloride-poor modifications thereof, the proportion of adherent viable cells was markedly increased compared to cryopreservation in cell culture medium (TiProtec: 38 ± 11%, modified TiProtec solutions ≥ 50%). Using these solutions, cells cryopreserved in a sub-confluent state were able to proliferate during re-culture. Mitochondrial fragmentation was observed in all solutions, but was partially reversible after cryopreservation in TiProtec and almost completely reversible in modified solutions within 3 h of re-culture. The superior protection of TiProtec and its modifications was apparent at all temperature gradients; however, best results were achieved with a cooling rate of -1°C/min. In conclusion, the use of TiProtec or modifications thereof as base solution for cryopreservation greatly improved cryopreservation results for endothelial monolayers in terms of survival and of monolayer and mitochondrial integrity.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 2","pages":"107-121"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1501136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36371763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Utility of extracellular matrix powders in tissue engineering. 细胞外基质粉末在组织工程中的应用。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-01 DOI: 10.1080/15476278.2018.1503771
Lauren Edgar, Afnan Altamimi, Marta García Sánchez, Riccardo Tamburrinia, Amish Asthana, Carlo Gazia, Giuseppe Orlando
{"title":"Utility of extracellular matrix powders in tissue engineering.","authors":"Lauren Edgar,&nbsp;Afnan Altamimi,&nbsp;Marta García Sánchez,&nbsp;Riccardo Tamburrinia,&nbsp;Amish Asthana,&nbsp;Carlo Gazia,&nbsp;Giuseppe Orlando","doi":"10.1080/15476278.2018.1503771","DOIUrl":"https://doi.org/10.1080/15476278.2018.1503771","url":null,"abstract":"<p><p>Extracellular matrix (ECM) materials have had remarkable success as scaffolds in tissue engineering (TE) and as therapies for tissue injury whereby the ECM microenvironment promotes constructive remodeling and tissue regeneration. ECM powder and solubilized derivatives thereof have novel applications in TE and RM afforded by the capacity of these constructs to be dynamically modulated. The powder form allows for effective incorporation and penetration of reagents; hence, ECM powder is an efficacious platform for 3D cell culture and vehicle for small molecule delivery. ECM powder offers minimally invasive therapy for tissue injury and successfully treatment for wounds refractory to first-line therapies. Comminution of ECM and fabrication of powder-derived constructs, however, may compromise the biological integrity of the ECM. The current lack of optimized fabrication protocols prevents a more extensive and effective clinical application of ECM powders. Further study on methods of ECM powder fabrication and modification is needed.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 4","pages":"172-186"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1503771","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10539053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Taurine enhances mouse cochlear neural stem cells proliferation and differentiation to sprial gangli through activating sonic hedgehog signaling pathway. 牛磺酸通过激活sonic hedgehog信号通路促进小鼠耳蜗神经干细胞向螺旋神经节的增殖和分化。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-13 DOI: 10.1080/15476278.2018.1477462
Xinghua Huang, Weijing Wu, Peng Hu, Qin Wang
{"title":"Taurine enhances mouse cochlear neural stem cells proliferation and differentiation to sprial gangli through activating sonic hedgehog signaling pathway.","authors":"Xinghua Huang,&nbsp;Weijing Wu,&nbsp;Peng Hu,&nbsp;Qin Wang","doi":"10.1080/15476278.2018.1477462","DOIUrl":"https://doi.org/10.1080/15476278.2018.1477462","url":null,"abstract":"<p><p>To investigate the molecular mechanism underlying taurine-stimulated proliferation and differentiation of cochlear neural stem cells (NSCs) and potential involvement of Sonic Hedgehog (Shh) pathway. The NSCs were characterized with immunofluorescence stained with nestin antibody. Cell viability was determined by MTT assay. The relative proliferation was measured by BrdU incorporation assay. The morphologic index was measured under light microscope. The relative protein level was determined by immunoblotting. Here we presented our findings that taurine stimulated proliferation and neurite outgrowth of NSCs, which was completely abolished by Shh inhibitor cyclopamine. In addition, cyclopamine antagonized taurine's effect on glutamatergic and GABAergic neuron population via suppressing expressions of Ptc-1, Smo and Gli-1. Our data supported the critical role of Shh pathway underlying the protective effect of taurine on auditory neural system.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 3","pages":"147-157"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1477462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36393666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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