Organogenesis最新文献

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Evaluation of Radiosterilized Glyercerolated Amniotic Membranes as a Substrate for Cultured Human Epithelial Cells. 放射性灭菌甘油三酯羊膜作为培养人上皮细胞底物的评价。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2020-01-01 Epub Date: 2020-02-15 DOI: 10.1080/15476278.2020.1723366
André O Paggiaro, Monica B Mathor, Walcy R Teodoro, Cesár Isaac, Vera L Capelozzi, Rolf Gemperli
{"title":"Evaluation of Radiosterilized Glyercerolated Amniotic Membranes as a Substrate for Cultured Human Epithelial Cells.","authors":"André O Paggiaro,&nbsp;Monica B Mathor,&nbsp;Walcy R Teodoro,&nbsp;Cesár Isaac,&nbsp;Vera L Capelozzi,&nbsp;Rolf Gemperli","doi":"10.1080/15476278.2020.1723366","DOIUrl":"https://doi.org/10.1080/15476278.2020.1723366","url":null,"abstract":"<p><p>Human amniotic membrane (HAM) is a biomaterial with biological properties beneficial to tissue repair, serving as a substrate for cell cultivation. Irradiation is used for tissue sterilization, but can damage the HAM structure. The objective of this paper was to construct a skin substitute, composed of human keratinocytes cultured on glycerolated HAMs, and to evaluate the influence radiation on subsequent cell culture growth. Four batches of HAMs were glycerolated, and half of them were radio-sterilzed with 25 kGy. Non-irradiated glycerolated HAM (ni-HAM) and irradiated glycerolated HAM (i-HAM) samples were then de-epithelized and analyzed using optical microscopy (Picrossirius staining), immunofluorescence and electron microscopy. Subsequently, keratinocytes were cultured on ni- and i-HAMs, and either immersed or positioned at the air-liquid interface. The basement membranes of the ni-HAM group remained intact following de-epithelialization, whereas the i-HAM group displayed no evidence or remnant presence of these membranes. Concerning the keratinocyte cultures, the ni-HAM substrate promoted the growth of multi-layered and differentiated epithelia. Keratinocytes cultured on i-HAM formed epithelium composed of three layers of stratification and discrete cell differentiation. The glycerolated HAM was compatible with cultured epithelia, demonstrating its potential as a skin substitute. Irradiation at 25 kGy caused structural damage to the amnion.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"16 1","pages":"27-41"},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2020.1723366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37648195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose–Collagen Gels 不同细胞类型和来源对纤维蛋白和琼脂糖-胶原凝胶血管形成前的影响
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-12-06 DOI: 10.1080/15476278.2019.1697597
Caroline Kniebs, F. Kreimendahl, M. Köpf, H. Fischer, S. Jockenhoevel, A. Thiebes
{"title":"Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose–Collagen Gels","authors":"Caroline Kniebs, F. Kreimendahl, M. Köpf, H. Fischer, S. Jockenhoevel, A. Thiebes","doi":"10.1080/15476278.2019.1697597","DOIUrl":"https://doi.org/10.1080/15476278.2019.1697597","url":null,"abstract":"ABSTRACT Vascularisation is essential for the development of tailored, tissue-engineered organs and tissues due to diffusion limits of nutrients and the lack of the necessary connection to the cardiovascular system. To pre-vascularize, endothelial cells and supporting cells can be embedded in the scaffold to foster an adequate nutrient and oxygen supply after transplantation. This technique is applied for tissue engineering of various tissues, but there have been few studies on the use of different cell types or cells sources. We compare the effect of supporting cells from different sources on vascularisation. Fibrin gels and agarose-collagen hydrogels were used as scaffolds. The supporting cells were primary human dermal fibroblasts (HDFs), human nasal fibroblasts (HNFs), human mesenchymal stem cells from umbilical cord’s Wharton’s jelly (WJ MSCs), adipose-derived MSCs (AD MSCs) and femoral bone marrow-derived MSCs (BM MSCs). The tissue constructs were incubated for 14 days and analyzed by two-photon laser scanning microscopy. Vascularisation was supported by all cell types, forming branched networks of tubular vascular structures in both hydrogels. In general, fibrin gels present a higher angiogenic promoting environment compared to agarose-collagen hydrogels and fibroblasts show a high angiogenic potential in co-culture with endothelial cells. In agarose-collagen hydrogels, vascular structures supported by AD MSCs were comparable to our HDF control in terms of volume, area and length. BM MSCs formed a homogeneous network of smaller structures in both hydrogels. This study provides data toward understanding the pre-vascularisation properties of different supporting cell types and sources for tissue engineering of different organs and tissues.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"16 1","pages":"14 - 26"},"PeriodicalIF":2.3,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1697597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46584709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Characterization and in vivo study of decellularized aortic scaffolds using closed sonication system 应用封闭超声系统对脱细胞主动脉支架的表征和体内研究
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-09-07 DOI: 10.1080/15476278.2019.1656997
A. Hazwani, M. Sha'ban, A. Azhim
{"title":"Characterization and in vivo study of decellularized aortic scaffolds using closed sonication system","authors":"A. Hazwani, M. Sha'ban, A. Azhim","doi":"10.1080/15476278.2019.1656997","DOIUrl":"https://doi.org/10.1080/15476278.2019.1656997","url":null,"abstract":"ABSTRACT Extracellular matrix (ECM) based bioscaffolds prepared by decellularization has increasingly emerged in tissue engineering application because it has structural, biochemical, and biomechanical cues that have dramatic effects upon cell behaviors. Therefore, we developed a closed sonication decellularization system to prepare ideal bioscaffolds with minimal adverse effects on the ECM. The decellularization was achieved at 170 kHz of ultrasound frequency in 0.1% and 2% Sodium Dodecyl Sulphate (SDS) solution for 10 hours. The immersion treatment as control was performed to compare the decellularization efficiency with our system. Cell removal and ECM structure were determined by histological staining and biochemical assay. Biomechanical properties were investigated by the indentation testing to test the stiffness, a residual force and compression of bioscaffolds. Additionally, in vivo implantation was performed in rat to investigate host tissue response. Compared to native tissues, histological staining and biochemical assay confirm the absence of cellularity with preservation of ECM structure. Moreover, sonication treatment has not affected the stiffness [N/mm] and a residual force [N] of the aortic scaffolds except for compression [%] which 2% SDS significantly decreased compared to native tissues showing higher SDS has a detrimental effect on ECM structure. Finally, minimal inflammatory response was observed after 1 and 5 weeks of implantation. This study reported that the novelty of our developed closed sonication system to prepare ideal bioscaffolds for tissue engineering applications.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"120 - 136"},"PeriodicalIF":2.3,"publicationDate":"2019-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1656997","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48202411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Advances of Wnt signalling pathway in dental development and potential clinical application Wnt信号通路在口腔发育中的研究进展及临床应用前景
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-09-04 DOI: 10.1080/15476278.2019.1656996
Xi Lu, Jun Yang, Shouliang Zhao, Shangfeng Liu
{"title":"Advances of Wnt signalling pathway in dental development and potential clinical application","authors":"Xi Lu, Jun Yang, Shouliang Zhao, Shangfeng Liu","doi":"10.1080/15476278.2019.1656996","DOIUrl":"https://doi.org/10.1080/15476278.2019.1656996","url":null,"abstract":"ABSTRACT Wnt signalling pathway is widely studied in many processes of biological development, like embryogenesis, tissue homeostasis and wound repair. It is universally known that Wnt signalling pathway plays an important role in tooth development. Here, we summarized the function of Wnt signalling pathway during tooth initiation, crown morphogenesis, root formation, and discussed the therapeutic potential of Wnt modulators.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"101 - 110"},"PeriodicalIF":2.3,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1656996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44866948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Specification of Sprouty2 functions in osteogenesis in in vivo context Sprouty2在体内成骨中的功能规范
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-09-04 DOI: 10.1080/15476278.2019.1656995
B. Veselá, E. Svandova, M. Hovořáková, R. Peterkova, A. Kratochvílová, Martina Pasovská, A. Ramešová, H. Lesot, E. Matalova
{"title":"Specification of Sprouty2 functions in osteogenesis in in vivo context","authors":"B. Veselá, E. Svandova, M. Hovořáková, R. Peterkova, A. Kratochvílová, Martina Pasovská, A. Ramešová, H. Lesot, E. Matalova","doi":"10.1080/15476278.2019.1656995","DOIUrl":"https://doi.org/10.1080/15476278.2019.1656995","url":null,"abstract":"ABSTRACT Sprouty proteins are modulators of the MAPK/ERK pathway. Amongst these, Sprouty2 (SPRY2) has been investigated as a possible factor that takes part in the initial phases of osteogenesis. However, the in vivo context has not yet been investigated and the underlying mechanisms taking place in vitro remain unknown. Therefore, in this study, the impact of Spry2 deficiency was examined in the developing tibias of Spry2 deficient (-/-) mouse. The investigation was performed when the osteogenic zone became clearly visible and when all three basic bone cells types were present. The main markers of osteoblasts, osteocytes and osteoclasts were evaluated by immunohistochemistry and RT-PCR. RT-PCR showed that the expression of Sost was 3.5 times higher in Spry2-/- than in the wild-type bone, which pointed to a still unknown mechanism of action of SPRY2 on the differentiation of osteocytes. The up-regulation of Sost was independent of Hif-1α expression and could not be related to its positive regulator, Runx2, since none of these factors showed an increased expression in the bone of Spry2-/- mice. Regarding the RANK/RANKL/OPG pathway, the Spry2-/- showed an increased expression of Rank, but no significant change in the expression of Rankl and Opg. Thanks to these results, the impact of Spry2 deletion is shown for the first time in the developing bone as a complex organ including, particularly, an effect on osteoblasts (Runx2) and osteocytes (Sost). This might explain the previously reported decrease in bone formation in postnatal Spry2-/- mice.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"111 - 119"},"PeriodicalIF":2.3,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1656995","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42520041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Expression and role of HIF-1α and HIF-2α in tissue regeneration: a study of hypoxia in house gecko tail regeneration HIF-1α和HIF-2α在组织再生中的表达及作用:缺氧对壁虎尾部再生的研究
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-07-03 DOI: 10.1080/15476278.2019.1644889
T. Novianti, V. Juniantito, A. A. Jusuf, E. Arida, S. A. Jusman, M. Sadikin
{"title":"Expression and role of HIF-1α and HIF-2α in tissue regeneration: a study of hypoxia in house gecko tail regeneration","authors":"T. Novianti, V. Juniantito, A. A. Jusuf, E. Arida, S. A. Jusman, M. Sadikin","doi":"10.1080/15476278.2019.1644889","DOIUrl":"https://doi.org/10.1080/15476278.2019.1644889","url":null,"abstract":"ABSTRACT The house gecko (Hemidactylus platyurus) has evolved the ability to autotomize its tail when threatened. The lost part is then regrown via epimorphic regeneration in a process that requires high energy and oxygen levels. Oxygen demand is therefore likely to outstrip supply and this can result in relative hypoxia in the tissues of the regenerating tail. The hypoxic state is stabilized by the Hypoxia Inducible Factor-1α (HIF-1α) and HIF-2α proteins. We induced tail autotomy in 30 mal H. platyurus adults using a standard procedure and then collected samples of the regenerated tail tissue on days 1, 3, 5, 8, 10, 13, 17, 21, 25, and 30 post autotomy. For each sample, mRNA expression was analyzed by qPCR, proteins were analyzed using Western Blot tests and immunohistochemistry, and the histological structure was analyzed using Hematoxylin and Eosin staining. On day 1, HIF-1α mRNA expression increased and the tissue was dominated by leucocyte and erythrocyte cells. HIF-1α mRNA expression peaked on day 3, at which time some cells were actively proliferating, migrating, and differentiating. At the same time as HIF-1α expression decreased, HIF-2α mRNA expression increased, as did overall cellular activity. HIF-2α expression increased more gradually but was present over a longer period of time than HIF-1α. We hypothesize that HIF-1α helps to initially stimulate the tissue regeneration process while HIF-2α functionally takes over the role of HIF-1α after HIF-1α succumbs to the oxygen conditions, but we suspect that both HIF-1α and HIF-2α play a role in overcoming the tissue’s hypoxic state.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"69 - 84"},"PeriodicalIF":2.3,"publicationDate":"2019-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1644889","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46259441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
In vivo construction of lymphoid node by implantation of adipose-derived stromal cells with hydroxypropyl methyl cellulose hydrogel in BALB/c nude mice 羟丙基甲基纤维素水凝胶植入脂肪基质细胞在BALB/c裸鼠体内构建淋巴结
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-07-03 DOI: 10.1080/15476278.2019.1656994
Jing Zhang, Yuqiao Xu, Tao Liu, Jie Min, Yu Ma, Yongli Song, Jianrong Lu, Wen-juan Mi, Yingmei Wang, Hang Li, Wangzhou Li, Da-Qing Zhao
{"title":"In vivo construction of lymphoid node by implantation of adipose-derived stromal cells with hydroxypropyl methyl cellulose hydrogel in BALB/c nude mice","authors":"Jing Zhang, Yuqiao Xu, Tao Liu, Jie Min, Yu Ma, Yongli Song, Jianrong Lu, Wen-juan Mi, Yingmei Wang, Hang Li, Wangzhou Li, Da-Qing Zhao","doi":"10.1080/15476278.2019.1656994","DOIUrl":"https://doi.org/10.1080/15476278.2019.1656994","url":null,"abstract":"ABSTRACT Adipose-derived stromal cells have multilineage potential to differentiate into several specialized tissue types. Herein, we investigated whether ADSCs could differentiate into lymphoid node in vivo. Human ADSCs from routine liposuction were cultured in differentiation medium and were supplemented with transforming growth factor β1 (TGF)-β1 and basic fibroblast growth factor (bFGF). The induced hADSCs mixed with 13% (w/v) hydroxypropyl methylcellulose (HPMC) were injected into BALB/c nude mice subcutaneously. Eight weeks later, nodules were found under the injected sites. Histology, immunohistochemistry, and species identification analysis confirmed that the nodules were lymphoid nodes that were derived from the injected hADSCs. Our experiment demonstrated that the hADSCs could differentiate into lymphocyte-like cells and form lymphoid nodes in vivo. TGF-β1 and bFGF might play important roles in the differentiation of hADSCs into lymphocyte-like cells. Our study might present an alternative approach for engineering immune organs and thus offer potential treatment for immunodeficiency diseases.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"85 - 99"},"PeriodicalIF":2.3,"publicationDate":"2019-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1656994","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48504660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Regeneration of caudal fin in Poecilia latipinna: Insights into the progressive tissue morphogenesis latipina Poecilia尾鳍的再生:对进行性组织形态发生的见解
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-04-03 DOI: 10.1080/15476278.2019.1633168
Sonam Patel, Isha Ranadive, I. Desai, S. Balakrishnan
{"title":"Regeneration of caudal fin in Poecilia latipinna: Insights into the progressive tissue morphogenesis","authors":"Sonam Patel, Isha Ranadive, I. Desai, S. Balakrishnan","doi":"10.1080/15476278.2019.1633168","DOIUrl":"https://doi.org/10.1080/15476278.2019.1633168","url":null,"abstract":"ABSTRACT Studies using fish fin as a model to understand the nuance of epimorphosis are gaining interest of lately. This study illustrates for the first time the daily changes in the tissue architecture of regenerating tail fin of Poecilia latipinna. Wound epithelium is formed within 24 hpa that eventually gets stratified into apical epithelial cap by 48 hpa. In the subsequent day, proliferating cells accumulate in front of each fin-ray marking the beginning of blastema. Distally these cells express signs of cartilage condensation by 4 dpa. However, ossification and subsequent transformation of actinotrichia to lepidotrichia was observed on 5 dpa. Subsequently, the regenerate grew at variable rate until it achieved the original size on 25 dpa. This result would serve as a worthwhile standard reference for further explorative studies that demand manipulation of a regulatory signal at a defined time point.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"35 - 42"},"PeriodicalIF":2.3,"publicationDate":"2019-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1633168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46993561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Anatomical structure, and expression of CCL4 and CCL13-like during the development of maxillary barbel in Paramisgurnus dabryanus. 大鳞副龙上颌倒钩发育过程中CCL4和ccl13样蛋白的表达及解剖结构。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-01-01 Epub Date: 2019-07-06 DOI: 10.1080/15476278.2019.1633870
Kianann Tan, Ruijing Geng, Zhiqiang Wang, Han Liu, Weimin Wang
{"title":"Anatomical structure, and expression of <i>CCL4</i> and <i>CCL13-like</i> during the development of maxillary barbel in <i>Paramisgurnus dabryanus</i>.","authors":"Kianann Tan,&nbsp;Ruijing Geng,&nbsp;Zhiqiang Wang,&nbsp;Han Liu,&nbsp;Weimin Wang","doi":"10.1080/15476278.2019.1633870","DOIUrl":"https://doi.org/10.1080/15476278.2019.1633870","url":null,"abstract":"<p><p><i>Paramisgurnus dabryanus</i> is one of the most economically important fishes in China. Barbels are an essential sensory organ for the food-seeking ability of teleost fish. However, the anatomical structure of the maxillary barbels of <i>P. dabryanus</i> and the molecular basis of their development are unknown. We investigated the anatomical structure of the barbel, and gene expression patterns of two chemokine C-C motif ligands: <i>CCL4</i> and <i>CCL13-like</i> during the maxillary barbel development using Masson Trichrome staining, light and electron microscopy, and qPCR. Anatomically, the maxillary barbel of <i>P. dabryanus</i> contains taste buds, melanophores, collagen fibers, connective tissue, smooth muscles, nerve bundles, and blood vessels, but does not have skeletal muscles or a skeleton rod. The expression of <i>CCL4</i> and <i>CCL13-like</i> was weak or non-existent in the early phases of development, but high at the last two studied time-points: 192- and 216-h post-hatching. Results indicated that <i>CCL4</i> and <i>CCL13-like</i> were related to the development of the maxillary barbel.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"13-23"},"PeriodicalIF":2.3,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1633870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37400639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ectopic localization of autophagosome in fatty liver is a key factor for liver regeneration. 脂肪肝中自噬体的异位定位是肝再生的关键因素。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2019-01-01 Epub Date: 2019-07-06 DOI: 10.1080/15476278.2019.1633872
Yoshihiro Matsumoto, Tomoharu Yoshizumi, Takeo Toshima, Kazuki Takeishi, Takasuke Fukuhara, Shinji Itoh, Toru Ikegami, Yuji Soejima, Masaki Mori
{"title":"Ectopic localization of autophagosome in fatty liver is a key factor for liver regeneration.","authors":"Yoshihiro Matsumoto,&nbsp;Tomoharu Yoshizumi,&nbsp;Takeo Toshima,&nbsp;Kazuki Takeishi,&nbsp;Takasuke Fukuhara,&nbsp;Shinji Itoh,&nbsp;Toru Ikegami,&nbsp;Yuji Soejima,&nbsp;Masaki Mori","doi":"10.1080/15476278.2019.1633872","DOIUrl":"https://doi.org/10.1080/15476278.2019.1633872","url":null,"abstract":"<p><p>Autophagy has a critical role in liver regeneration. However, no studies have demonstrated autophagic flux in the regenerating fatty liver. The aim of this study was to clarify the dynamics of autophagy in the regeneration of the fatty liver. Following 70% partial hepatectomy (PH) in db/db fatty mice, which is a non-alcoholic fatty liver disease (NAFLD) model, we investigated the survival rate and recovery of liver volume. Histological examination of the regenerating liver was examined using electron microscopy. The 7-day survival rate after PH in db/db mice was 20%, which was significantly lower than that in control mice (<i>P</i>< .01). Liver regeneration within 48 h after PH was significantly impaired in db/db mice (<i>P</i>< .05). The number of proliferating cell nuclear antigen (PCNA) positive cells and the expression levels of cell-cycle markers cyclins D, E, and A were lower in db/db mice compared with controls. In the regenerating liver, LC3-II level was higher in db/db mice, but p62 expression was increased and cathepsin D expression, a marker of autophagolysosome proteolysis, was decreased compared with controls. Additionally, electronic microscopy revealed that autophagosomes during liver regeneration in db/db mice were mainly located in lipid droplets. Our findings indicate that the different localization of autophagosomes in db/db mice compared with controls led to impairment of liver regeneration in the fatty liver.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 1","pages":"24-34"},"PeriodicalIF":2.3,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1633872","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37137743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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