Organogenesis最新文献_第6页

ATP6V1H facilitates osteogenic differentiation in MC3T3-E1 cells via Akt/GSK3β signaling pathway. ATP6V1H通过Akt/GSK3β信号通路促进MC3T3-E1细胞成骨分化。

IF 2.3 4区生物学

Organogenesis Pub Date : 2019-01-01 Epub Date: 2019-07-04 DOI: 10.1080/15476278.2019.1633869

Fusong Jiang, Haojie Shan, Chenhao Pan, Zubin Zhou, Keze Cui, Yuanliang Chen, Haibo Zhong, Zhibin Lin, Nan Wang, Liang Yan, Xiaowei Yu

{"title":"ATP6V1H facilitates osteogenic differentiation in MC3T3-E1 cells via Akt/GSK3β signaling pathway.","authors":"Fusong Jiang, Haojie Shan, Chenhao Pan, Zubin Zhou, Keze Cui, Yuanliang Chen, Haibo Zhong, Zhibin Lin, Nan Wang, Liang Yan, Xiaowei Yu","doi":"10.1080/15476278.2019.1633869","DOIUrl":"https://doi.org/10.1080/15476278.2019.1633869","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) accounts for approximately 90% of all diabetic patients, and osteoporosis is one of the complications during T2DM process. ATP6V1H (V-type proton ATPase subunit H) displays crucial roles in inhibiting bone loss, but its role in osteogenic differentiation remains unknown. Therefore in this study, we aimed to explore the biological role of ATP6V1H in osteogenic differentiation. OM (osteogenic medium) and HG (high glucose and free fatty acids) were used to induce the MC3T3-E1 cells into osteogenic differentiation in a T2DM simulating environment. CCK8 assay was used to detect cell viability. Alizarin Red staining was used to detect the influence of ATP6V1H on osteogenic differentiation. ATP6V1H expression increased in OM-MC3T3-E1 cells, while decreased in OM+HG-MC3T3-E1 cells. ATP6V1H promoted osteogenic differentiation of OM+HG-MC3T3-E1 cells. Overexpression of ATP6V1H inhibited Akt/GSK3β signaling pathway, while knockdown of ATP6V1H promoted Akt/GSK3β signaling pathway. ATP6V1H overexpression promoted osteogenic differentiation of OM+HG-MC3T3-E1 cells. The role of ATP6V1H in osteogenic differentiation in a T2DM simulating environment involved in Akt/GSK3β signaling pathway. These data demonstrated that ATP6V1H could serve as a potential target for osteogenic differentiation in a T2DM simulating environment.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"15 2","pages":"43-54"},"PeriodicalIF":2.3,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2019.1633869","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37130242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Corrigendum. 勘误表。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-01-30 DOI: 10.1080/15476278.2017.1422894

引用次数: 0

Suppression of LRRC19 promotes cutaneous wound healing in pressure ulcers in mice. 抑制LRRC19促进小鼠压疮皮肤创面愈合。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-02-20 DOI: 10.1080/15476278.2018.1436924

Jie Sun, Zhijing Wang, Xirui Wang

{"title":"Suppression of LRRC19 promotes cutaneous wound healing in pressure ulcers in mice.","authors":"Jie Sun, Zhijing Wang, Xirui Wang","doi":"10.1080/15476278.2018.1436924","DOIUrl":"https://doi.org/10.1080/15476278.2018.1436924","url":null,"abstract":"The ischemia-reperfusion (I/R) induced skin lesion has been identified as primary cause of pressure ulcer. Better understanding of the mechanism is required for new therapy development. Leucine rich repeat containing protein 19 (LRRC19) is a recently discovered transmembrane protein containing leucine-rich repeats and plays a role in immune response. To investigate the role of LRRC19 in pressure ulcers, mouse ulcer model was established with two cycles of I/R. The expression of LRRC19 was assessed during injury. siRNA mediated LRRC19 downregulation was applied to investigate the disease severity, immune cell infiltration and pro-inflammatory cytokines production. The primary skin fibroblasts were stimulated with IL-1β to dissect the molecular mechanism. LRRC19 was readily induced in I/R induced lesion site in a pattern mimicking the disease progress as measured by wound area. Knockdown of LRRC19 by siRNA significantly alleviated the disease severity and attenuated immune cell infiltration and pro-inflammatory cytokines production. In primary skin fibroblast model, siRNA knockdown of LRRC19 suppressed IL-1β mediated NFκB activation and its downstream cytokines production. LRRC19 was a novel factor for I/R-induced tissue damage by promoting NFκB dependent pro-inflammatory response. Our results supported that LRRC19 could be a potential therapeutic target for pressure ulcers.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 1","pages":"13-24"},"PeriodicalIF":2.3,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1436924","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35847320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Embryonic skin development and repair. 胚胎皮肤发育和修复。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-02-15 DOI: 10.1080/15476278.2017.1421882

Michael S Hu, Mimi R Borrelli, Wan Xing Hong, Samir Malhotra, Alexander T M Cheung, Ryan C Ransom, Robert C Rennert, Shane D Morrison, H Peter Lorenz, Michael T Longaker

引用次数: 0

Erratum. 勘误表。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-02 Epub Date: 2017-12-06 DOI: 10.1080/15476278.2017.1407509

引用次数: 0

Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells. 猪脱细胞真皮基质处理对伤口愈合和瘢痕形成的影响:表皮干细胞中Jag1表达的作用

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-04-27 DOI: 10.1080/15476278.2018.1436023

Xiao-Dong Chen, Shu-Bin Ruan, Ze-Peng Lin, Ziheng Zhou, Feng-Gang Zhang, Rong-Hua Yang, Ju-Lin Xie

{"title":"Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells.","authors":"Xiao-Dong Chen, Shu-Bin Ruan, Ze-Peng Lin, Ziheng Zhou, Feng-Gang Zhang, Rong-Hua Yang, Ju-Lin Xie","doi":"10.1080/15476278.2018.1436023","DOIUrl":"https://doi.org/10.1080/15476278.2018.1436023","url":null,"abstract":"Skin wound healing involves Notch/Jagged1 signaling. However, little is known how Jag1 expression level in epidermal stem cells (ESCs) contributes to wound healing and scar formation. We applied multiple cellular and molecular techniques to examine how Jag1 expression in ESCs modulates ESCs differentiation to myofibroblasts (MFB) in vitro, interpret how Jag1 expression in ESCs is involved in wound healing and scar formation in mice, and evaluate the effects of porcine acellular dermal matrix (ADM) treatment on wound healing and scar formation. We found that Jag1, Notch1 and Hes1 expression was up-regulated in the wound tissue during the period of wound healing. Furthermore, Jag1 expression level in the ESCs was positively associated with the level of differentiation to MFB. ESC-specific knockout of Jag1 delayed wound healing and promoted scar formation in vivo. In addition, we reported that porcine ADM treatment after skin incision could accelerate wound closure and reduce scar formation in vivo. This effect was associated with decreased expression of MFB markers, including α-SMA Col-1 and Col-III in wound tissues. Finally, we confirmed that porcine ADM treatment could increase Jag1, Notch1 and Hesl expression in wound tissues. Taken together, our results suggested that ESC-specific Jag1 expression levels are critical for wound healing and scar formation, and porcine ADM treatment would be beneficial in promoting wound healing and preventing scar formation by enhancing Notch/Jagged1 signaling pathway in ESCs.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 1","pages":"25-35"},"PeriodicalIF":2.3,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1436023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35810874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures. 3D打印结构中原发性肝细胞维持的肝脏特异性功能延长。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-02-01 DOI: 10.1080/15476278.2018.1423931

Yohan Kim, Kyojin Kang, Sangtae Yoon, Ji Sook Kim, Su A Park, Wan Doo Kim, Seung Bum Lee, Ki-Young Ryu, Jaemin Jeong, Dongho Choi

{"title":"Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures.","authors":"Yohan Kim, Kyojin Kang, Sangtae Yoon, Ji Sook Kim, Su A Park, Wan Doo Kim, Seung Bum Lee, Ki-Young Ryu, Jaemin Jeong, Dongho Choi","doi":"10.1080/15476278.2018.1423931","DOIUrl":"https://doi.org/10.1080/15476278.2018.1423931","url":null,"abstract":"Isolated primary hepatocytes from the liver are very similar to in vivo native liver hepatocytes, but they have the disadvantage of a limited lifespan in 2D culture. Although a sandwich culture and 3D organoids with mesenchymal stem cells (MSCs) as an attractive assistant cell source to extend lifespan can be used, it cannot fully reproduce the in vivo architecture. Moreover, long-term 3D culture leads to cell death because of hypoxic stress. Therefore, to overcome the drawback of 2D and 3D organoids, we try to use a 3D printing technique using alginate hydrogels with primary hepatocytes and MSCs. The viability of isolated hepatocytes was more than 90%, and the cells remained alive for 7 days without morphological changes in the 3D hepatic architecture with MSCs. Compared to a 2D system, the expression level of functional hepatic genes and proteins was higher for up to 7 days in the 3D hepatic architecture. These results suggest that both the 3D bio-printing technique and paracrine molecules secreted by MSCs supported long-term culture of hepatocytes without morphological changes. Thus, this technique allows for widespread expansion of cells while forming multicellular aggregates, may be applied to drug screening and could be an efficient method for developing an artificial liver.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 1","pages":"1-12"},"PeriodicalIF":2.3,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1423931","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35759824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Sodium hydroxide based non-detergent decellularizing solution for rat lung. 大鼠肺用氢氧化钠非去污剂脱细胞液。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-11 DOI: 10.1080/15476278.2018.1462432

Hideyori Sengyoku, Tomoshi Tsuchiya, Tomohiro Obata, Ryoichiro Doi, Yasumasa Hashimoto, Mitsutoshi Ishii, Hiromi Sakai, Naoto Matsuo, Daisuke Taniguchi, Takashi Suematsu, Murray Lawn, Keitaro Matsumoto, Takuro Miyazaki, Takeshi Nagayasu

{"title":"Sodium hydroxide based non-detergent decellularizing solution for rat lung.","authors":"Hideyori Sengyoku, Tomoshi Tsuchiya, Tomohiro Obata, Ryoichiro Doi, Yasumasa Hashimoto, Mitsutoshi Ishii, Hiromi Sakai, Naoto Matsuo, Daisuke Taniguchi, Takashi Suematsu, Murray Lawn, Keitaro Matsumoto, Takuro Miyazaki, Takeshi Nagayasu","doi":"10.1080/15476278.2018.1462432","DOIUrl":"https://doi.org/10.1080/15476278.2018.1462432","url":null,"abstract":"Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches are possible. Here we used high alkaline (pH12) sodium hydroxide (NaOH)-PBS solution without detergents for lung decellularization and compared the efficacy on DNA elimination and ECM preservation with detergent based decellularization solutions CHAPS and SDS. Immunohistochemical image analysis showed that cell components were removed by NaOH solution as well as other detergents. A Collagen and GAG assay showed that the collagen reduction of the NaOH group was comparable to that of the CHAPS and SDS groups. However, DNA reduction was more significant in the NaOH group than in other groups (p < 0.0001). The recellularization of HUVEC revealed cell attachment was not inferior to that of the SDS group. Ex vivo functional analysis showed 100% oxygen ventilation increased oxygen partial pressure as artificial hemoglobin vesicle-PBS solution passed through regenerated lungs in the SDS or NaOH group. It was concluded that the NaOH-PBS based decellularization solution was comparable to ordinal decellularizaton solutions and competitive in cost effectiveness and residues in the decellularized scaffold negligible, thus providing another potential option to detergent for future clinical usage.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 2","pages":"94-106"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1462432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36211225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Bioinspired liver scaffold design criteria. 仿生肝支架设计标准。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-29 DOI: 10.1080/15476278.2018.1505137

Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia

{"title":"Bioinspired liver scaffold design criteria.","authors":"Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia","doi":"10.1080/15476278.2018.1505137","DOIUrl":"10.1080/15476278.2018.1505137","url":null,"abstract":"Maintaining hepatic functional characteristics in-vitro is considered one of the main challenges in engineering liver tissue. As hepatocytes cultured ex-vivo are deprived of their native extracellular matrix (ECM) milieu, developing scaffolds that mimic the biomechanical and physicochemical properties of the native ECM is thought to be a promising approach for successful tissue engineering and regenerative medicine applications. On the basis that the decellularized liver matrix represents the ideal design template for engineering bioinspired hepatic scaffolds, to derive quantitative descriptors of liver ECM architecture, we characterised decellularised liver matrices in terms of their biochemical, viscoelastic and structural features along with porosity, permeability and wettability. Together, these data provide a unique set of quantitative design criteria which can be used to generate guidelines for fabricating biomaterial scaffolds for liver tissue engineering. As proof-of-concept, we investigated hepatic cell response to substrate viscoelasticity. On collagen hydrogels mimicking decellularised liver mechanics, cells showed superior morphology, higher viability and albumin secretion than on stiffer and less viscous substrates. Although scaffold properties are generally inspired by those of native tissues, our results indicate significant differences between the mechano-structural characteristics of untreated and decellularised hepatic tissue. Therefore, we suggest that design rules - such as mechanical properties and swelling behaviour - for engineering biomimetic scaffolds be re-examined through further studies on substrates matching the features of decellularized liver matrices.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 3","pages":"129-146"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1505137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36441030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection. 一种用于高灵敏度细胞分泌组检测的微流控竞争性免疫聚集试验。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-08 DOI: 10.1080/15476278.2018.1461306

Fan Liu, Pawan Kc, Liwei Ni, Ge Zhang, Jiang Zhe

{"title":"A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection.","authors":"Fan Liu, Pawan Kc, Liwei Ni, Ge Zhang, Jiang Zhe","doi":"10.1080/15476278.2018.1461306","DOIUrl":"https://doi.org/10.1080/15476278.2018.1461306","url":null,"abstract":"We report a high-sensitivity cell secretome detection method using competitive immuno-aggregation and a micro-Coulter counter. A target cell secretome protein competes with anti-biotin-coated microparticles (MPs) to bind with a biotinylated antibody (Ab), causing decreased aggregation of the functionalized MPs and formation of a mixture of MPs and aggregates. In comparison, without the target cell secretome protein, more microparticles are functionalized, and more aggregates are formed. Thus, a decrease in the average volume of functionalized microparticles/aggregates indicates an increase in cell secretome concentration. This volume change is measured by the micro-Coulter counter, which is used to quantitatively estimate the cell secretome concentration. Vascular endothelial growth factor (VEGF), one of the key cell secretome proteins that regulate angiogenesis and vascular permeabilization, was used as the target protein to demonstrate the sensing principle. A standard calibration curve was generated by testing samples with various VEGF concentrations. A detection range from 0.01 ng/mL to 100.00 ng/mL was achieved. We further demonstrated the quantification of VEGF concentration in exogenous samples collected from the secretome of human mesenchymal stem cells (hMSCs) at different incubation times. The results from the assay agree well with the results of a parallel enzyme-linked immunoabsorbent assay (ELISA) test, indicating the specificity and reliability of the competitive immuno-aggregation assay. With its simple structure and easy sample preparation, this assay not only enables high sensitivity detection of VEGF but also can be readily extended to other types of cell secretome analysis as long as the specific Ab is known.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"14 2","pages":"67-81"},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1461306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36206372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5