Organogenesis最新文献_第7页

Sodium hydroxide based non-detergent decellularizing solution for rat lung. 大鼠肺用氢氧化钠非去污剂脱细胞液。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-11 DOI: 10.1080/15476278.2018.1462432

Hideyori Sengyoku, Tomoshi Tsuchiya, Tomohiro Obata, Ryoichiro Doi, Yasumasa Hashimoto, Mitsutoshi Ishii, Hiromi Sakai, Naoto Matsuo, Daisuke Taniguchi, Takashi Suematsu, Murray Lawn, Keitaro Matsumoto, Takuro Miyazaki, Takeshi Nagayasu

{"title":"Sodium hydroxide based non-detergent decellularizing solution for rat lung.","authors":"Hideyori Sengyoku, Tomoshi Tsuchiya, Tomohiro Obata, Ryoichiro Doi, Yasumasa Hashimoto, Mitsutoshi Ishii, Hiromi Sakai, Naoto Matsuo, Daisuke Taniguchi, Takashi Suematsu, Murray Lawn, Keitaro Matsumoto, Takuro Miyazaki, Takeshi Nagayasu","doi":"10.1080/15476278.2018.1462432","DOIUrl":"https://doi.org/10.1080/15476278.2018.1462432","url":null,"abstract":"Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches are possible. Here we used high alkaline (pH12) sodium hydroxide (NaOH)-PBS solution without detergents for lung decellularization and compared the efficacy on DNA elimination and ECM preservation with detergent based decellularization solutions CHAPS and SDS. Immunohistochemical image analysis showed that cell components were removed by NaOH solution as well as other detergents. A Collagen and GAG assay showed that the collagen reduction of the NaOH group was comparable to that of the CHAPS and SDS groups. However, DNA reduction was more significant in the NaOH group than in other groups (p < 0.0001). The recellularization of HUVEC revealed cell attachment was not inferior to that of the SDS group. Ex vivo functional analysis showed 100% oxygen ventilation increased oxygen partial pressure as artificial hemoglobin vesicle-PBS solution passed through regenerated lungs in the SDS or NaOH group. It was concluded that the NaOH-PBS based decellularization solution was comparable to ordinal decellularizaton solutions and competitive in cost effectiveness and residues in the decellularized scaffold negligible, thus providing another potential option to detergent for future clinical usage.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1462432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36211225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Rebuilding a better home for transplanted islets. 为移植的胰岛重建一个更好的家园。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-09-25 DOI: 10.1080/15476278.2018.1517509

Daniel M Tremmel, Jon S Odorico

引用次数: 18

Bioinspired liver scaffold design criteria. 仿生肝支架设计标准。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-29 DOI: 10.1080/15476278.2018.1505137

Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia

{"title":"Bioinspired liver scaffold design criteria.","authors":"Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia","doi":"10.1080/15476278.2018.1505137","DOIUrl":"10.1080/15476278.2018.1505137","url":null,"abstract":"Maintaining hepatic functional characteristics in-vitro is considered one of the main challenges in engineering liver tissue. As hepatocytes cultured ex-vivo are deprived of their native extracellular matrix (ECM) milieu, developing scaffolds that mimic the biomechanical and physicochemical properties of the native ECM is thought to be a promising approach for successful tissue engineering and regenerative medicine applications. On the basis that the decellularized liver matrix represents the ideal design template for engineering bioinspired hepatic scaffolds, to derive quantitative descriptors of liver ECM architecture, we characterised decellularised liver matrices in terms of their biochemical, viscoelastic and structural features along with porosity, permeability and wettability. Together, these data provide a unique set of quantitative design criteria which can be used to generate guidelines for fabricating biomaterial scaffolds for liver tissue engineering. As proof-of-concept, we investigated hepatic cell response to substrate viscoelasticity. On collagen hydrogels mimicking decellularised liver mechanics, cells showed superior morphology, higher viability and albumin secretion than on stiffer and less viscous substrates. Although scaffold properties are generally inspired by those of native tissues, our results indicate significant differences between the mechano-structural characteristics of untreated and decellularised hepatic tissue. Therefore, we suggest that design rules - such as mechanical properties and swelling behaviour - for engineering biomimetic scaffolds be re-examined through further studies on substrates matching the features of decellularized liver matrices.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1505137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36441030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection. 一种用于高灵敏度细胞分泌组检测的微流控竞争性免疫聚集试验。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-08 DOI: 10.1080/15476278.2018.1461306

Fan Liu, Pawan Kc, Liwei Ni, Ge Zhang, Jiang Zhe

{"title":"A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection.","authors":"Fan Liu, Pawan Kc, Liwei Ni, Ge Zhang, Jiang Zhe","doi":"10.1080/15476278.2018.1461306","DOIUrl":"https://doi.org/10.1080/15476278.2018.1461306","url":null,"abstract":"We report a high-sensitivity cell secretome detection method using competitive immuno-aggregation and a micro-Coulter counter. A target cell secretome protein competes with anti-biotin-coated microparticles (MPs) to bind with a biotinylated antibody (Ab), causing decreased aggregation of the functionalized MPs and formation of a mixture of MPs and aggregates. In comparison, without the target cell secretome protein, more microparticles are functionalized, and more aggregates are formed. Thus, a decrease in the average volume of functionalized microparticles/aggregates indicates an increase in cell secretome concentration. This volume change is measured by the micro-Coulter counter, which is used to quantitatively estimate the cell secretome concentration. Vascular endothelial growth factor (VEGF), one of the key cell secretome proteins that regulate angiogenesis and vascular permeabilization, was used as the target protein to demonstrate the sensing principle. A standard calibration curve was generated by testing samples with various VEGF concentrations. A detection range from 0.01 ng/mL to 100.00 ng/mL was achieved. We further demonstrated the quantification of VEGF concentration in exogenous samples collected from the secretome of human mesenchymal stem cells (hMSCs) at different incubation times. The results from the assay agree well with the results of a parallel enzyme-linked immunoabsorbent assay (ELISA) test, indicating the specificity and reliability of the competitive immuno-aggregation assay. With its simple structure and easy sample preparation, this assay not only enables high sensitivity detection of VEGF but also can be readily extended to other types of cell secretome analysis as long as the specific Ab is known.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1461306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36206372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution. 无血清和无白蛋白的内皮单层低温保存新方法。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-06 DOI: 10.1080/15476278.2018.1501136

Gesine Pless-Petig, Sven Knoop, Ursula Rauen

{"title":"Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution.","authors":"Gesine Pless-Petig, Sven Knoop, Ursula Rauen","doi":"10.1080/15476278.2018.1501136","DOIUrl":"https://doi.org/10.1080/15476278.2018.1501136","url":null,"abstract":"Cryopreservation is the only long-term storage option for the storage of vessels and vascular constructs. However, endothelial barrier function is almost completely lost after cryopreservation in most established cryopreservation solutions. We here aimed to improve endothelial function after cryopreservation using the 2D-model of porcine aortic endothelial cell monolayers. The monolayers were cryopreserved in cell culture medium or cold storage solutions based on the 4°C vascular preservation solution TiProtec®, all supplemented with 10% DMSO, using different temperature gradients. After short-term storage at -80°C, monolayers were rapidly thawed and re-cultured in cell culture medium. Thawing after cryopreservation in cell culture medium caused both immediate and delayed cell death, resulting in 11 ± 5% living cells after 24 h of re-culture. After cryopreservation in TiProtec and chloride-poor modifications thereof, the proportion of adherent viable cells was markedly increased compared to cryopreservation in cell culture medium (TiProtec: 38 ± 11%, modified TiProtec solutions ≥ 50%). Using these solutions, cells cryopreserved in a sub-confluent state were able to proliferate during re-culture. Mitochondrial fragmentation was observed in all solutions, but was partially reversible after cryopreservation in TiProtec and almost completely reversible in modified solutions within 3 h of re-culture. The superior protection of TiProtec and its modifications was apparent at all temperature gradients; however, best results were achieved with a cooling rate of -1°C/min. In conclusion, the use of TiProtec or modifications thereof as base solution for cryopreservation greatly improved cryopreservation results for endothelial monolayers in terms of survival and of monolayer and mitochondrial integrity.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1501136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36371763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Utility of extracellular matrix powders in tissue engineering. 细胞外基质粉末在组织工程中的应用。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 DOI: 10.1080/15476278.2018.1503771

Lauren Edgar, Afnan Altamimi, Marta García Sánchez, Riccardo Tamburrinia, Amish Asthana, Carlo Gazia, Giuseppe Orlando

{"title":"Utility of extracellular matrix powders in tissue engineering.","authors":"Lauren Edgar, Afnan Altamimi, Marta García Sánchez, Riccardo Tamburrinia, Amish Asthana, Carlo Gazia, Giuseppe Orlando","doi":"10.1080/15476278.2018.1503771","DOIUrl":"https://doi.org/10.1080/15476278.2018.1503771","url":null,"abstract":"Extracellular matrix (ECM) materials have had remarkable success as scaffolds in tissue engineering (TE) and as therapies for tissue injury whereby the ECM microenvironment promotes constructive remodeling and tissue regeneration. ECM powder and solubilized derivatives thereof have novel applications in TE and RM afforded by the capacity of these constructs to be dynamically modulated. The powder form allows for effective incorporation and penetration of reagents; hence, ECM powder is an efficacious platform for 3D cell culture and vehicle for small molecule delivery. ECM powder offers minimally invasive therapy for tissue injury and successfully treatment for wounds refractory to first-line therapies. Comminution of ECM and fabrication of powder-derived constructs, however, may compromise the biological integrity of the ECM. The current lack of optimized fabrication protocols prevents a more extensive and effective clinical application of ECM powders. Further study on methods of ECM powder fabrication and modification is needed.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1503771","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10539053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Taurine enhances mouse cochlear neural stem cells proliferation and differentiation to sprial gangli through activating sonic hedgehog signaling pathway. 牛磺酸通过激活sonic hedgehog信号通路促进小鼠耳蜗神经干细胞向螺旋神经节的增殖和分化。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-13 DOI: 10.1080/15476278.2018.1477462

Xinghua Huang, Weijing Wu, Peng Hu, Qin Wang

引用次数: 4

Celastrol enhances Atoh1 expression in inner ear stem cells and promotes their differentiation into functional auditory neuronal-like cells. Celastrol增强内耳干细胞中Atoh1的表达，促进其向功能性听觉神经元样细胞分化。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-06-14 DOI: 10.1080/15476278.2018.1462433

Zhao Han, Yu-Yan Gu, Ning Cong, Rui Ma, Fang-Lu Chi

{"title":"Celastrol enhances Atoh1 expression in inner ear stem cells and promotes their differentiation into functional auditory neuronal-like cells.","authors":"Zhao Han, Yu-Yan Gu, Ning Cong, Rui Ma, Fang-Lu Chi","doi":"10.1080/15476278.2018.1462433","DOIUrl":"https://doi.org/10.1080/15476278.2018.1462433","url":null,"abstract":"We aimed to investigate the beneficial effect of Celastrol on inner ear stem cells and potential therapeutic value for hearing loss. The inner ear stem cells were isolated and characterized from utricular sensory epithelium of adult mice. The stemness was evaluated by sphere formation assay. The relative expressions of Atoh1, MAP-2 and Myosin VI were measured by RT-PCR and immunoblotting. The up-regulation of MAP-2 was also analysed with immunofluorescence. The in vitro neuronal excitability was interrogated by calcium oscillation. The electrophysiological property was determined by inward current recorded on patch clamp. Our results demonstrated that Celastrol treatment significantly improved the viability and proliferation of mouse inner ear stem cells, and facilitated sphere formation. Moreover, Celastrol stimulated differentiation of mouse inner ear stem cells to neuronal-like cells and enhanced neural excitability. Celastrol also enhanced neuronal-like cell identity in the inner ear stem cell derived neurons, as well as their electrophysiological function. Most notably, these effects were apparently associated with the upregulation of Atoh1 in response to Celastrol treatment. Celastrol showed beneficial effect on inner ear stem cells and held therapeutic promise against hearing loss.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2018.1462433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36221934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Application of drug delivery systems for the controlled delivery of growth factors to treat nervous system injury. 药物递送系统在生长因子控制递送治疗神经系统损伤中的应用。

IF 2.3 4区生物学

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-27 DOI: 10.1080/15476278.2018.1491183

Fukai Ma, Fan Wang, Ronggang Li, Jianhong Zhu

引用次数: 0

Regulation of proliferation in developing human tooth germs by MSX homeodomain proteins and cyclin-dependent kinase inhibitor p19^INK4d. MSX同源结构域蛋白和周期蛋白依赖性激酶抑制剂p19INK4d对人牙胚增殖的调控

IF 2.3 4区生物学

Organogenesis Pub Date : 2017-10-02 Epub Date: 2017-09-21 DOI: 10.1080/15476278.2017.1358337

Darko Kero, Katarina Vukojevic, Petra Stazic, Danijela Sundov, Snjezana Mardesic Brakus, Mirna Saraga-Babic

{"title":"Regulation of proliferation in developing human tooth germs by MSX homeodomain proteins and cyclin-dependent kinase inhibitor p19INK4d.","authors":"Darko Kero, Katarina Vukojevic, Petra Stazic, Danijela Sundov, Snjezana Mardesic Brakus, Mirna Saraga-Babic","doi":"10.1080/15476278.2017.1358337","DOIUrl":"https://doi.org/10.1080/15476278.2017.1358337","url":null,"abstract":"Before the secretion of hard dental tissues, tooth germs undergo several distinctive stages of development (dental lamina, bud, cap and bell). Every stage is characterized by specific proliferation patterns, which is regulated by various morphogens, growth factors and homeodomain proteins. The role of MSX homeodomain proteins in odontogenesis is rather complex. Expression domains of genes encoding for murine Msx1/2 during development are observed in tissues containing highly proliferative progenitor cells. Arrest of tooth development in Msx knockout mice can be attributed to impaired proliferation of progenitor cells. In Msx1 knockout mice, these progenitor cells start to differentiate prematurely as they strongly express cyclin-dependent kinase inhibitor p19INK4d. p19INK4d induces terminal differentiation of cells by blocking the cell cycle in mitogen-responsive G1 phase. Direct suppression of p19INK4d by Msx1 protein is, therefore, important for maintaining proliferation of progenitor cells at levels required for the normal progression of tooth development. In this study, we examined the expression patterns of MSX1, MSX2 and p19INK4d in human incisor tooth germs during the bud, cap and early bell stages of development. The distribution of expression domains of p19INK4d throughout the investigated period indicates that p19INK4d plays active role during human tooth development. Furthermore, comparison of expression domains of p19INK4d with those of MSX1, MSX2 and proliferation markers Ki67, Cyclin A2 and pRb, indicates that MSX-mediated regulation of proliferation in human tooth germs might not be executed by the mechanism similar to one described in developing tooth germs of wild-type mouse.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2017-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2017.1358337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35428356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14