Organogenesis最新文献

{"title":"Adipose-derived mesenchymal stem cells-derived exosomes containing nano-pearl powder water-soluble matrix promote osteogenic differentiation of MC3T3-E1 cells.","authors":"Ling Chen, QiuHua Mao, WenBai Zhang, YaNan Cheng","doi":"10.1080/15476278.2026.2630547","DOIUrl":"10.1080/15476278.2026.2630547","url":null,"abstract":"<p><strong>Objective: </strong>To explore the synergistic effect of nano-pearl powder (NPP) and adipose-derived stem cell exosomes (ADSC-Exos) on the osteogenic potential of MC3T3-E1 cells.</p><p><strong>Methods: </strong>The water-soluble matrix of NPP (NPP-WSM) was extracted via freeze-drying, and ADSC-Exos were isolated by ultracentrifugation. NPP-WSM was incorporated into ADSC-Exos through co-incubation to generate NPP-WSM-Exos. MC3T3-E1 cells were treated with NPP-WSM or NPP-WSM-Exos. Cell proliferation and migration were evaluated using CCK-8 and wound-healing assays, respectively. Osteogenic differentiation was assessed by Alizarin Red S staining and alkaline phosphatase (ALP) activity. The expression of osteogenesis-related genes (COL1A1, RUNX2, OCN, and OPN) was measured by qPCR and Western blotting. Transcriptome sequencing (RNA-seq) was conducted to identify signaling pathways activated by NPP-WSM-Exos.</p><p><strong>Results: </strong>NPP-WSM-Exos displayed distinct exosome morphology and biomarkers, confirming their successful preparation. Significantly, NPP-WSM-Exos enhanced the viability of MC3T3-E1 cells compared to NPP-WSM alone and upregulated the expression of osteogenic genes, including COL1A1, RUNX2, OCN, and OPN, at both the transcriptional and translational levels. Additionally, NPP-WSM-Exos strongly promoted mineralization, as evidenced by the increased calcification observed through Alizarin Red S staining, and elevated alkaline phosphatase (ALP) activity, indicating excellent potential for osteogenic differentiation. Transcriptome sequencing showed that NPP-WSM-Exos significantly enhanced the PI3K/AKT pathway in MC3T3-E1 cells, while protein level detection indicated that NPP-WSM-Exos could increase AKT phosphorylation levels and inhibit GSK3β activity to improve osteogenic efficiency.</p><p><strong>Conclusion: </strong>The use of adipose-derived stem cell exosomes to encapsulate NPP-WSM can increase the utilization of WSM, promote the proliferation of MC3T3-E1, and enhance the osteogenic differentiation ability.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"22 1","pages":"2630547"},"PeriodicalIF":2.8,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12947552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147308609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of TX-100/SDS-based decellularized vascular material using ultrasound and chemical treatment: evaluation of structure and biosafety.","authors":"Hongguang Chen, Xiaomei Bie, Lifang Hao, HaiGang Jia, Xiufen Li, Chunli Zhang, Jianmei Guo","doi":"10.1080/15476278.2025.2575599","DOIUrl":"10.1080/15476278.2025.2575599","url":null,"abstract":"<p><p>Decellularized blood vessels with low immunogenicity and excellent biocompatibility are promising for tissue engineering and clinical applications. However, current decellularization methods face limitations in cell removal efficiency, matrix preservation, and biosafety. This study optimized the Triton X-100/SDS (TX-100/SDS) decellularization method using ultrasound technology by systematically evaluating the effects of ultrasound power, temperature, and processing time on decellularization efficiency. The optimized method achieved a 72% reduction in nucleic acid residues at 100 W power while preserving matrix integrity and significantly reducing chemical reagent residues. Structural and biosafety evaluations confirmed that the optimized scaffolds met biological safety standards and demonstrated excellent stability, providing a strong foundation for developing high-performance decellularized vascular materials for clinical applications.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"22 1","pages":"2575599"},"PeriodicalIF":2.8,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145952751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of vascular remodeling between a bioresorbable poly-L-lactic acid scaffold and a bare metal stent: a 6-month angiography and intravascular ultrasound analysis in porcine iliac arteries.","authors":"Keita Hayashi, Hideaki Obara, Naoki Fujimura, Yohei Masugi, Yasuhito Sekimoto, Kentaro Matsubara, Yuko Kitagawa","doi":"10.1080/15476278.2026.2630543","DOIUrl":"10.1080/15476278.2026.2630543","url":null,"abstract":"<p><p>Animal experimental studies involving the Igaki-Tamai stent (ITS), a bioresorbable poly-l-lactic acid scaffold, in peripheral arteries are limited, and existing studies evaluated only short-term (3-month) outcomes. This study compared arterial responses associated with the ITS and bare metal stent (BMS) over 6 months using intravascular ultrasound (IVUS) analysis and evaluated feasibility in porcine iliac arteries. Four miniature pigs underwent stent implantation with the ITS in the right iliac artery and the BMS in the left iliac artery. Follow-up evaluations at 6, 12, and 24 weeks included angiographic and IVUS analyses to assess neointimal hyperplasia, percent area stenosis (%AS), and percent in-stent volume obstruction (%VO). Histological analysis was performed to evaluate tissue injury and inflammation scores. At 6 weeks, the neointimal area did not differ significantly between the ITS and BMS groups (8.49 ± 2.10 mm² vs 13.47 ± 6.67 mm², <i>P</i> = .205). However, the ITS group exhibited a significantly smaller neointimal area at 12 weeks (6.87 ± 1.15 mm² vs 20.65 ± 10.99 mm², <i>P</i> = .050) and 24 weeks (5.20 ± 0.85 mm² vs 22.32 ± 12.03 mm², <i>P</i> = .042). %AS and %VO were significantly lower in the ITS group at all follow-ups. The ITS group showed reduced tissue damage (injury score: 0.80 ± 0.430 vs 1.74 ± 0.908, <i>P</i> < .001) and inflammation (inflammation score: 1.25 ± 0.516 vs 1.67 ± 0.832, <i>P</i> < .001) compared with the BMS group. The ITS was associated with reduced vessel injury, lower inflammatory response, and favorable luminal remodeling over 6 months in healthy porcine iliac arteries.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"22 1","pages":"2630543"},"PeriodicalIF":2.8,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12915771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MLPH/RAB3A accelerates the differentiation of pancreatic stem cells to islet β-cells to control blood glucose in diabetic rats.","authors":"Junling Shan, Huifeng Wang, Guangyu Zhu","doi":"10.1080/15476278.2026.2630542","DOIUrl":"10.1080/15476278.2026.2630542","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to determine the potential mechanism by which pancreatic stem cell-derived beta cells (PSCs-β) assist in the body's glucose-lowering capacity in type 1 diabetes (T1D) rats.</p><p><strong>Methods: </strong>We screened the transcriptomic changes in the pancreatic islets of T1D mice to extract key genes from the GSE169275 dataset. Cell proliferation, cell cycle distribution, apoptosis, PSC-β differentiation ability, and insulin production levels were analyzed after MLPH overexpression/knockdown in PSCs. PSC-β-overexpressing MLPH were transplanted into T1D rats, and the changes in fasting blood glucose level, glucose tolerance and insulin, glucagon and C-peptide contents were examined. After the target genes of MLPH were analysed using the database, immunoprecipitation was introduced for validation. Whether RAB3A is involved in the regulatory effects of MLPH on the proliferation and differentiation of PSCs was further verified.</p><p><strong>Results: </strong>MLPH overexpression further enhanced the proliferation of PSCs, inhibited apoptosis and accelerated the differentiation of PSCs to PSC-β cells and insulin secretion. After the transplantation of MLPH-overexpressing PSC-β cells, the pancreatic islet tissue damage was restored, the insulin expression was substantially elevated and the glucagon content decreased. RAB3A knockdown counteracted the effects of MLPH on the proliferation, differentiation and insulin secretion of PSCs.</p><p><strong>Conclusion: </strong>MLPH overexpression is favourable for the differentiation of PSCs to insulin β-cells. Transplantation of MLPH-overexpressing PSC-β cells restored the ability of T1D rats to manage a glycemic load by promoting RAB3A expression.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"22 1","pages":"2630542"},"PeriodicalIF":2.8,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12959181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel tissue-engineered stent graft combining decellularized scaffold and bioresorbable stent: a pilot feasibility study in a porcine model.","authors":"Tatsuya Shimogawara, Kentaro Matsubara, Kazuki Tajima, Masayuki Shimoda, Hiroshi Yagi, Hideaki Obara, Yuko Kitagawa","doi":"10.1080/15476278.2025.2610591","DOIUrl":"10.1080/15476278.2025.2610591","url":null,"abstract":"<p><p>Endovascular aneurysm repair (EVAR) is a widely accepted treatment for aortic pathologies owing to its minimally invasive nature. However, long-term complications, such as stent graft migration and infection, remain unresolved, primarily due to the persistent presence of synthetic materials and limited tissue integration. This pilot study evaluated the feasibility of a novel tissue-engineered stent graft (TESG) combining a bioresorbable poly-L-lactic acid (PLLA) stent with decellularized porcine veins. The veins were processed using a sodium dodecyl sulfate and the Triton X-100 decellularization protocol. Histological and ultrastructural analyses confirmed effective cell removal while preserving extracellular matrix components. Quantitative deoxyribonucleic acid (DNA) analysis showed a > 97% reduction in DNA content. The TESGs were assembled by suturing the decellularized veins into bioresorbable PLLA stents and implanted into porcine iliac arteries (<i>n</i> = 3). Commercially available prosthetic grafts were used as control implants to evaluate differences in tissue responses. Graft patency and morphology were assessed at implantation and on postoperative day 14 using angiography and intravascular ultrasonography. All TESGs remained patent, with no evidence of thrombosis or aneurysmal changes. Histological analysis revealed early endothelialization and smooth muscle cell infiltration within the TESG wall, in contrast to the prosthetic graft controls, which lacked comparable cellular integration. This study demonstrated the short-term feasibility and biological compatibility of a fully bioresorbable TESG. Although long-term outcomes remain to be established, these results support further development of TESG to reduce late complications through improved tissue integration and avoidance of permanent synthetic materials.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"22 1","pages":"2610591"},"PeriodicalIF":2.8,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12758350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145864413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of CGF in osteogenic differentiation of periodontal ligament stem cells through the Wnt pathway.","authors":"Bing Zhou, Wentu Zhou, Xin Li, Cheng Wang","doi":"10.1080/15476278.2025.2575622","DOIUrl":"10.1080/15476278.2025.2575622","url":null,"abstract":"<p><strong>Objective: </strong>Periodontal ligament stem cells (PDLSCs), undifferentiated mesenchymal cells with multipotent differentiation and self-renewal capacities, constitute the optimal MSC population for periodontal regeneration. This study sought to elucidate the effects of concentrated growth factor (CGF) on the osteogenic differentiation of PDLSCs and analyze the underlying mechanisms.</p><p><strong>Methods: </strong>PDLSCs were isolated from the molars of patients with malocclusion and characterized by flow cytometry, osteogenic induction, lipogenic induction, ARS staining and ORO staining. PDLSCs were treated with osteogenic induction medium containing different concentrations of CGF. The osteogenic ability of CGF in PDLSCs was analyzed via ALP staining, ARS staining, and ALP activity assays. WNK1, RUNX2 and OPN were detected by RT-qPCR. WNK1, RUNX2, OPN, <i>β</i>-catenin, GSK3β and <i>p</i>-GSK3β were detected by WB. The role of CGF in PDLSC osteogenic differentiation through the Wnt pathway was verified.</p><p><strong>Results: </strong>PDLSCs were successfully isolated and cultured in vitro. After CGF treatment, ALP activity, mineralization nodule formation, and the expression of RUNX2 and OPN in PDLSCs were increased, with 0.1 mg/mL CGF showing the best osteogenic differentiation ability. WNK1, <i>β</i>-catenin, and <i>p</i>-GSK3β/GSK3β were elevated. CGF activated the Wnt pathway through WNK1. The promoting effects of CGF on osteogenic differentiation of PDLSCs were partially reversed after inhibition of WNK1 and the Wnt pathway.</p><p><strong>Conclusion: </strong>CGF promotes PDLSC osteogenic differentiation by activating the Wnt pathway through WNK1.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"21 1","pages":"2575622"},"PeriodicalIF":2.8,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145513287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal miR-29a-3p derived from bone marrow stromal cells suppresses malignant behavior of NSCLC by regulating DNMT3A/JAK2/STAT3 axis.","authors":"Chao Su, Xiaoliang Han, Kang Zhang","doi":"10.1080/15476278.2025.2575590","DOIUrl":"10.1080/15476278.2025.2575590","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) can be transported to tumor cells through exosomes secreted by bone marrow mesenchymal stem cells (BMSC-Exos) and exert regulatory functions within cells. Here, we aim to investigate the functional mechanism of miR-29a-3p carried by BMSC-Exos in the treatment of NSCLC. Based on the miRNA/mRNA gene expression data in the UCSC dataset (1029 NSCLC and 110 normal samples), bioinformatics analysis predicted the expression levels of miR-29a-3p and DNMT3A in NSCLC samples and their association with prognosis. Exosomes were isolated from BMSCs and characterized. BMSC-Exos with expressed or knocked out miR-29a-3p were treated A549 cells, and their biological effects on cells were evaluated, including proliferation, migration, and apoptosis. Western blotting was employed to explore the involvement of DNMT3A and JAK2/STAT3 signaling pathways. Furthermore, the binding between miR-29a-3p and DNMT3A was verified through dual-luciferase reporter assay. Following transfection with miR-29a-3p mimic and DNMT3A overexpression vectors, their roles in the biological processes of NSCLC were analyzed. In NSCLC, decreased expression of miR-29a-3p or increased expression of DNMT3A was closely associated with poor prognosis. miR-29a-3p can be transferred from BMSCs to A549 cells via exosomes, thereby inhibiting cell proliferation and migration while promoting apoptosis. DNMT3A was identified as a target gene of miR-29a-3p. Mechanistically, miR-29a-3p upregulation led to decreased DNMT3A expression and impaired JAK2/STAT3 signaling pathway. Overall, this study demonstrated that BMSC-derived exosomal miR-29a-3p restrained NSCLC by reducing DNMT3A/JAK2/STAT3 axis. These findings may provide new insights for the development of NSCLC treatment strategies.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"21 1","pages":"2575590"},"PeriodicalIF":2.8,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145496172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalein Alleviates Lithium-Pilocarpine-Induced Status Epilepticus by Regulating DNMT1/GABRD Pathway in Rats.","authors":"Zhenggang Wu, Jing Liu, Deju Yin, Jing Huang, Yujing Huang, Pengfei Wang","doi":"10.1080/15476278.2025.2519607","DOIUrl":"10.1080/15476278.2025.2519607","url":null,"abstract":"<p><strong>Background: </strong>Epilepsy is a common disease of the nervous system. Recent advances in epigenetics have revealed DNA methylation as a key mechanism in epilepsy pathogenesis, particularly through dysregulation of GABAergic signaling. Baicalein has been shown to have anticonvulsant and neuroprotective effects. However, its epigenetic regulatory effects on GABA receptor function remain unexplored.</p><p><strong>Methods: </strong>The status epilepticus (SE) model was induced by lithium chloride-pilocarpine (LiCl-PILO) in Sprague-Dawley (SD) rats. The rats were divided into control group, epileptic SE group and baicalein intervention group. Morris water maze (MWM) test, Nissl staining, immunofluorescence and enzyme-linked immunosorbent assay (ELISA) were used to detect cognitive functions and neuronal damage. Online sites, chromatin immunoprecipitation (ChIP) and western blotting were used to identify DNA methyltransferase 1 (DNMT1)-mediated methylation of gamma-aminobutyric acid type A receptor subunit delta (GABRD) promoter region.</p><p><strong>Results: </strong>Baicalein treatment significantly prolonged the latency of SE onset and seizure onset, and improved the development of epilepsy. Meanwhile, baicalein improved the cognitive impairment in rats induced by LiCl-PILO. After treatment with baicalein, a sustained elevation in the number of neurons and NeuN levels was observed, along with a decrease in the contents of tumor necrosis factor -alpha (TNF-α), interleukin-1β (IL-1β), and ionized calcium-binding adapter molecule 1 (Iba-1) in the hippocampus. Mechanistically, baicalein interacted with DNMT1 to suppress GABRD promoter region methylation, thus increasing GABRD protein level in the hippocampus of rats induced by LiCl-PILO.</p><p><strong>Conclusion: </strong>This study identifies DNMT1/GABRD axis as a novel epigenetic target for epilepsy intervention. Baicalein's ability to enhance tonic inhibition through demethylation of GABRD provides a groundbreaking strategy for drug-resistant epilepsy.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"21 1","pages":"2519607"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pnky Modulates Neural Stem Cell Proliferation and Differentiation Through Activation of Wnt/β-Catenin Signaling Pathway.","authors":"Haidong Wu, Jing Huang, Xiaojing Li, Yali Song, Xuxiang Chen, Yajie Guo","doi":"10.1080/15476278.2025.2519641","DOIUrl":"10.1080/15476278.2025.2519641","url":null,"abstract":"<p><p>Neural stem cell (NSC) possess the essential properties of pluripotency and self-renewal, making them promising candidates for the treatment of neurological disorders such as Alzheimer's disease (AD), Parkinson's disease (PD), and spinal cord injuries. While previous studies have identified the long non-coding RNAs (lncRNAs) Pnky as a regulator of NSC differentiation into neurons via RNA splicing, its role in NSC differentiation and proliferation through the Wnt/β-catenin pathway remains unclear. In this study, we investigated the mechanism by which Pnky influences the Wnt/β-catenin pathway to promote NSC differentiation into neurons. Using cck8 assays, western blot analysis, and quantitative polymerase chain reaction (qPCR), we found that Pnky knockdown significantly enhanced NSC proliferation and promoted their differentiation into neurons. Additionally, Pnky knockdown resulted in the downregulation of the neural stem cell marker Nestin and upregulation of the neuronal marker β3-Tubulin, through activation of the β-catenin signaling pathway. Conversely, inhibiting the β-catenin pathway hindered both NSC differentiation and proliferation. These findings suggest that targeting the Pnky-mediated Wnt/β-catenin pathway may offer novel strategies for the treatment, diagnosis, and drug development of central nervous system diseases.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"21 1","pages":"2519641"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12184132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized Individualized Nursing Improves Recovery and Reduces Complications in ICU Patients with Severe Pneumonia.","authors":"Linjuan Wu, Jingchuan Lin","doi":"10.1080/15476278.2025.2489670","DOIUrl":"10.1080/15476278.2025.2489670","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluates the effectiveness of optimized individualized nursing interventions on clinical outcomes in intensive care unit (ICU) patients with severe pneumonia.</p><p><strong>Methods: </strong>In this randomized controlled trial, 76 patients with severe pneumonia were randomized into a control group and an experimental group. Both groups received routine nursing care. On this basis, the experimental group received optimized individualized nursing. After the nursing intervention, clinical outcomes, respiratory function, coagulation function, Acute Physiology and Chronic Health Evaluation II (APACHE II) score, and St. George's Respiratory Problems Questionnaire (SGRQ) score were assessed, and the complication and mortality rates were counted.</p><p><strong>Results: </strong>After the intervention, compared with the control group, the experimental group exhibited shorter times of fever reduction, white blood cell count recovery, and off-boarding and ICU stay, higher oxygenation index, lower rapid shallow breathing index, respiratory rate, activated partial thromboplastin time, prothrombin time, fibrinogen, and D-Dimer levels, lower APACHE II scores and SGRQ scores (<i>p</i> < 0.05). Additionally, the experimental group possessed a lower complication rate and mortality rate than the control group (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Implementing optimized individualized nursing can significantly enhance recovery and reduce complications in ICU patients with severe pneumonia.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"21 1","pages":"2489670"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}