Organogenesis最新文献

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Meckel Gruber syndrome, A case report. 梅克尔-格鲁伯综合征,一例报告。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 Epub Date: 2015-06-02 DOI: 10.1080/15476278.2015.1055431
Kiper Aslan, Elif Külahçı Aslan, Adnan Orhan, Mehmet Aral Atalay
{"title":"Meckel Gruber syndrome, A case report.","authors":"Kiper Aslan,&nbsp;Elif Külahçı Aslan,&nbsp;Adnan Orhan,&nbsp;Mehmet Aral Atalay","doi":"10.1080/15476278.2015.1055431","DOIUrl":"https://doi.org/10.1080/15476278.2015.1055431","url":null,"abstract":"<p><strong>Introduction: </strong>Meckel-Gruber Syndrome was first described by J R Meckel in 1822. It is an autosomal recessive disorder, and is caused by the failure of mesodermal induction. The typical triad of Meckel-Gruber Syndrome (MGS) involves meningo-encephalocele, polycystic kidneys and postaxial polydactyly. The worldwide incidence varies from 1 in 1.300 to 1 in 140.000 live births.</p><p><strong>Case: </strong>In this report, we present a case of MGS in which the diagnosis was made at 19 weeks of gestation based on ultrasonographic findings of the typical triad of the disease (encephalocele, polycystic kidneys, and polydactyly) These features were suggestive of the diagnosis of Meckel Gruber Syndrome (MGS). She had also placenta previa totalis. The patient was counselled regarding the lethal outcome of MGS. Unfortunately, the family did not approve the termination of pregnancy. At the 32nd week, she referred to hospital with complaints of vaginal bleeding and uterine contractions. An emergency cesarean section was perfomed due to plasental malposition. A 1380 gr, female fetus was delivered. First and 5th minute Apgar scores were 1 and 0, respectively. Consequently, the baby died after 45 minutes of neonatal resuscitation.</p><p><strong>Conclusion: </strong>MGS is a lethal disorder. One cannot speak about survival of the fetus because of the pulmonary hypoplasia. The parents should be counseled about prognosis of the fetus and the outcome. Counselers should strictly give information about the recurrence risk for the next pregnancies.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 2","pages":"87-92"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1055431","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33355750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Wnt signaling induces epithelial differentiation during cutaneous wound healing. Wnt信号在皮肤伤口愈合过程中诱导上皮分化。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1086052
Khosrow S Houschyar, Arash Momeni, Malcolm N Pyles, Zeshaan N Maan, Alexander J Whittam, Frank Siemers
{"title":"Wnt signaling induces epithelial differentiation during cutaneous wound healing.","authors":"Khosrow S Houschyar,&nbsp;Arash Momeni,&nbsp;Malcolm N Pyles,&nbsp;Zeshaan N Maan,&nbsp;Alexander J Whittam,&nbsp;Frank Siemers","doi":"10.1080/15476278.2015.1086052","DOIUrl":"https://doi.org/10.1080/15476278.2015.1086052","url":null,"abstract":"<p><p>Cutaneous wound repair in adult mammals typically does not regenerate original dermal architecture. Skin that has undergone repair following injury is not identical to intact uninjured skin. This disparity may be caused by differences in the mechanisms that regulate postnatal cutaneous wound repair compared to embryonic skin development and thus we seek a deeper understanding of the role that Wnt signaling plays in the mechanisms of skin repair in both fetal and adult wounds. The influence of secreted Wnt signaling proteins in tissue homeostasis has galvanized efforts to identify small molecules that target Wnt-mediated cellular responses. Wnt signaling is activated by wounding and participates in every subsequent stage of the healing process from the control of inflammation and programmed cell death, to the mobilization of stem cell reservoirs within the wound site. Endogenous Wnt signaling augmentation represents an attractive option to aid in the restoration of cutaneous wounds, as the complex mechanisms of the Wnt pathway have been increasingly investigated over the years. In this review, we summarize recent data elucidating the roles that Wnt signaling plays in cutaneous wound healing process. </p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 3","pages":"95-104"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1086052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33953216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 71
Freezing/Thawing without Cryoprotectant Damages Native but not Decellularized Porcine Renal Tissue. 无冷冻保护剂的冷冻/解冻会损害原生而非脱细胞的猪肾组织。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1022009
Nafiseh Poornejad, Timothy S Frost, Daniel R Scott, Brinden B Elton, Paul R Reynolds, Beverly L Roeder, Alonzo D Cook
{"title":"Freezing/Thawing without Cryoprotectant Damages Native but not Decellularized Porcine Renal Tissue.","authors":"Nafiseh Poornejad,&nbsp;Timothy S Frost,&nbsp;Daniel R Scott,&nbsp;Brinden B Elton,&nbsp;Paul R Reynolds,&nbsp;Beverly L Roeder,&nbsp;Alonzo D Cook","doi":"10.1080/15476278.2015.1022009","DOIUrl":"https://doi.org/10.1080/15476278.2015.1022009","url":null,"abstract":"<p><p>Whole organ decellularization of porcine renal tissue and recellularization with a patient's own cells would potentially overcome immunorejection, which is one of the most significant problems with allogeneic kidney transplantation. However, there are obstacles to achieving this goal, including preservation of the decellularized extracellular matrix (ECM), identifying the proper cell types, and repopulating the ECM before transplantation. Freezing biological tissue is the best option to avoid spoilage; however, it may damage the structure of the tissue or disrupt cellular membranes through ice crystal formation. Cryoprotectants have been used to repress ice formation during freezing, although cell toxicity can still occur. The effect of freezing/thawing on native (n = 10) and decellularized (n = 10) whole porcine kidneys was studied without using cryoprotectants. Results showed that the elastic modulus of native kidneys was reduced by a factor of 22 (P < 0.0001) by freezing/thawing or decellularization, while the elastic modulus for decellularized ECM was essentially unchanged by the freezing/thawing process (p = 0.0636). Arterial pressure, representative of structural integrity, was also reduced by a factor of 52 (P < 0.0001) after freezing/thawing for native kidneys, compared to a factor of 43 (P < 0.0001) for decellularization and a factor of 4 (P < 0.0001) for freezing/thawing decellularized structures. Both freezing/thawing and decellularization reduced stiffness, but the reductions were not additive. Investigation of the microstructure of frozen/thawed native and decellularized renal tissues showed increased porosity due to cell removal and ice crystal formation. Orcein and Sirius staining showed partial damage to elastic and collagen fibers after freezing/thawing. It was concluded that cellular damage and removal was more responsible for reducing stiffness than fibril destruction. Cell viability and growth were demonstrated on decellularized frozen/thawed and non-frozen samples using human renal cortical tubular epithelial (RCTE) cells over 12 d. No adverse effect on the ability to recellularize after freezing/thawing was observed. It is recommended that porcine kidneys be frozen prior to decellularization to prevent contamination, and after decellularization to prevent protein denaturation. Cryoprotectants may still be necessary, however, during storage and transportation after recellularization. </p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 1","pages":"30-45"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1022009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33096298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Vascular replacement using a layered elastin-collagen vascular graft in a porcine model: one week patency versus one month occlusion. 在猪模型中使用层状弹性胶原血管移植物进行血管置换:通畅一周vs闭塞一个月。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 Epub Date: 2015-06-10 DOI: 10.1080/15476278.2015.1038448
M J W Koens, A G Krasznai, A E J Hanssen, T Hendriks, R Praster, W F Daamen, J A van der Vliet, T H van Kuppevelt
{"title":"Vascular replacement using a layered elastin-collagen vascular graft in a porcine model: one week patency versus one month occlusion.","authors":"M J W Koens,&nbsp;A G Krasznai,&nbsp;A E J Hanssen,&nbsp;T Hendriks,&nbsp;R Praster,&nbsp;W F Daamen,&nbsp;J A van der Vliet,&nbsp;T H van Kuppevelt","doi":"10.1080/15476278.2015.1038448","DOIUrl":"https://doi.org/10.1080/15476278.2015.1038448","url":null,"abstract":"<p><p>A persistent clinical demand exists for a suitable arterial prosthesis. In this study, a vascular conduit mimicking the native 3-layered artery, and constructed from the extracellular matrix proteins type I collagen and elastin, was evaluated for its performance as a blood vessel equivalent. A tubular 3-layered graft (elastin-collagen-collagen) was prepared using highly purified type I collagen fibrils and elastin fibers, resembling the 3-layered native blood vessel architecture. The vascular graft was crosslinked and heparinised (37 ± 4 μg heparin/mg graft), and evaluated as a vascular graft using a porcine bilateral iliac artery model. An intra-animal comparison with clinically-used heparinised ePTFE (Propaten®) was made. Analyses included biochemical characterization, duplex scanning, (immuno)histochemistry and scanning electron microscopy. The tubular graft was easy to handle with adequate suturability. Implantation resulted in pulsating grafts without leakage. One week after implantation, both ePTFE and the natural acellular graft had 100% patencies on duplex scanning. Grafts were partially endothelialised (Von Willebrand-positive endothelium with a laminin-positive basal membrane layer). After one month, layered thrombi were found in the natural (4/4) and ePTFE graft (1/4), resulting in occlusion which in case of the natural graft is likely due to the porosity of the inner elastin layer. In vivo application of a molecularly-defined tubular graft, based on nature's matrix proteins, for vascular surgery is feasible.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 3","pages":"105-21"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1038448","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33377070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts. 丝素海绵涂层对针织丝小直径血管移植物体内性能的影响。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1093268
Toshiharu Fukayama, Yusuke Ozai, Haruka Shimokawadoko, Derya Aytemiz, Ryou Tanaka, Noboru Machida, Tetsuo Asakura
{"title":"Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts.","authors":"Toshiharu Fukayama,&nbsp;Yusuke Ozai,&nbsp;Haruka Shimokawadoko,&nbsp;Derya Aytemiz,&nbsp;Ryou Tanaka,&nbsp;Noboru Machida,&nbsp;Tetsuo Asakura","doi":"10.1080/15476278.2015.1093268","DOIUrl":"https://doi.org/10.1080/15476278.2015.1093268","url":null,"abstract":"<p><p>Vascular grafts under 5 mm or less in diameter are not developed due to a problem caused by early thrombus formation, neointimal hyperplasia, etc. Bombyx mori silk fibroin (SF) which has biodegradability and tissue infiltration is focused as tube and coating material of vascular grafts. Coating is an important factor to maintain the strength of the anastomotic region of vascular grafts, and to prevent the blood leak from the vascular grafts after implantation. Therefore, in this research, we focused on the SF concentration of the coating solution, and tissue infiltration and remodeling were compared among each SF concentration. Silk poly (-ethylene) glycol diglycidyl ether (PGDE) coating with concentrations of 1.0%, 2.5%, 5.0%, and 7.5% SF were applied for the double-raschel knitted small-sized vessel with 1.5 mm diameter and 1cm in length. The grafts were implanted in the rat abdominal aorta and removed after 3 weeks or 3 months. Vascular grafts patency was monitored by ultrasound, and morphological evaluation was performed by histopathological examination. SF concentration had no significant effects on the patency rate. However, tissue infiltration was significantly higher in the sample of 2.5% SF in 3 weeks, and 1.0% and 2.5% SF in 3 months. Also, in comparison of length inside of the graft, stenosis were not found in 3 weeks, however, found with 5.0% and 7.5% in 3 months. From these results, it is clear that 2.5% SF coating is the most suitable concentration, based on the characteristics of less stenosis, early tissue infiltration, and less neointimal hyperplasia.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 3","pages":"137-51"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1093268","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34113437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Application of retinoic acid improves form and function of tissue engineered corneal construct. 应用维甲酸可改善组织工程角膜结构的形态和功能。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1093267
Fadhilah Z Abidin, Ricardo M Gouveia, Che J Connon
{"title":"Application of retinoic acid improves form and function of tissue engineered corneal construct.","authors":"Fadhilah Z Abidin, Ricardo M Gouveia, Che J Connon","doi":"10.1080/15476278.2015.1093267","DOIUrl":"10.1080/15476278.2015.1093267","url":null,"abstract":"<p><p>Retinoic acid has recently been shown to control the phenotype and extracellular matrix composition of corneal stromal cells cultured in vitro as monolayers. This study set out to investigate the effects of retinoic acid on human corneal keratocytes within a 3D environment. Human corneal keratocytes were encapsulated in collagen gels, which were subsequently compressed under load, and cultured in serum-free media supplemented with 10 µM retinoic acid or DMSO vehicle for 30 days. Cell proliferation was quantified on selected days, while the expression of several important keratocytes markers was evaluated at day 30 using RT-PCR and immunoblotting. The weight and size of the collagen constructs were measured before and after hydration and contraction analyses. Retinoic acid enhanced keratocyte proliferation until day 30, whereas cells in control culture conditions showed reduced numbers after day 21. Both gene and protein expressions of keratocyte-characteristic proteoglycans (keratocan, lumican and decorin), corneal crystallins and collagen type I and V were significantly increased following retinoic acid supplementation. Retinoic acid also significantly reduced the expression of matrix metalloproteases 1, 3 and 9 while not increasing α-smooth muscle actin and fibronectin expression. Furthermore, these effects were also correlated with the ability of retinoic acid to significantly inhibit the contractility of keratocytes while allowing the build-up of corneal stromal extracellular matrix within the 3D constructs. Thus, retinoic acid supplementation represents a promising strategy to improve the phenotype of 3D-cultured keratocytes, and their usefulness as a model of corneal stroma for corneal biology and regenerative medicine applications. </p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 3","pages":"122-36"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34113436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Creation and implantation of acellular rat renal ECM-based scaffolds. 脱细胞大鼠肾内皮细胞基质支架的制备与植入。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1072661
Andrea Peloso, Jacopo Ferrario, Benedetta Maiga, Ilaria Benzoni, Carolina Bianco, Antonio Citro, Manuela Currao, Alessandro Malara, Annalisa Gaspari, Alessandra Balduini, Massimo Abelli, Lorenzo Piemonti, Paolo Dionigi, Giuseppe Orlando, Marcello Maestri
{"title":"Creation and implantation of acellular rat renal ECM-based scaffolds.","authors":"Andrea Peloso,&nbsp;Jacopo Ferrario,&nbsp;Benedetta Maiga,&nbsp;Ilaria Benzoni,&nbsp;Carolina Bianco,&nbsp;Antonio Citro,&nbsp;Manuela Currao,&nbsp;Alessandro Malara,&nbsp;Annalisa Gaspari,&nbsp;Alessandra Balduini,&nbsp;Massimo Abelli,&nbsp;Lorenzo Piemonti,&nbsp;Paolo Dionigi,&nbsp;Giuseppe Orlando,&nbsp;Marcello Maestri","doi":"10.1080/15476278.2015.1072661","DOIUrl":"https://doi.org/10.1080/15476278.2015.1072661","url":null,"abstract":"<p><p>Kidney transplantation is the only potentially curative treatment for patient facing end-stage renal disease, and it is now routinely used. Its use is mainly limited by the supply of transplantable donor organs, which far exceeds the demand. Regenerative medicine and tissue engineering offer promising means for overcoming this shortage. In the present study, we developed and validated a protocol for producing acellular rat renal scaffolds. Left kidneys were removed from 26 male Lewis rats (weights: 250-350 g) and decellularized by means of aortic anterograde perfusion with ionic and anionic detergents (Triton X-100 1% and SDS 1%, respectively). 19 scaffolds thus obtained (and contralateral native kidneys as controls) were deeply characterized in order to evaluate the decellularization quality, the preservation of extracellular matrix components and resultant micro-angioarchitecture structure. The other 7 were transplanted into 7 recipient rats that had undergone unilateral nephrectomy. Recipients were sacrificed on post-transplantation day 7 and the scaffolds subjected to histologic studies. The dual-detergent protocol showed, with only 5 h of perfusion per organ, to obtain thoroughly decellularized renal scaffolds consisting almost exclusively of extracellular matrix. Finally the macro- and the microarchitecture of the renal parenchyma were well preserved, and the grafts were implanted with ease. Seven days after transplant, the scaffolds were morphologically intact although all vascular structures were obstructed with thrombi. Production and implantation of acellular rat renal scaffolds is a suitable platform for further studies on regenerative medicine and tissue engineering.</p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 2","pages":"58-74"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1072661","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34299364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
Erratum. 勘误表。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1063374
{"title":"Erratum.","authors":"","doi":"10.1080/15476278.2015.1063374","DOIUrl":"https://doi.org/10.1080/15476278.2015.1063374","url":null,"abstract":"","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 2","pages":"93"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1063374","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33971422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Mesenchymal stem cells: potential for therapy and treatment of chronic non-healing skin wounds. 间充质干细胞:治疗和治疗慢性不愈合皮肤伤口的潜力。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1126018
Giovanni Marfia, Stefania Elena Navone, Clara Di Vito, Nicola Ughi, Silvia Tabano, Monica Miozzo, Carlo Tremolada, Gianni Bolla, Chiara Crotti, Francesca Ingegnoli, Paolo Rampini, Laura Riboni, Roberta Gualtierotti, Rolando Campanella
{"title":"Mesenchymal stem cells: potential for therapy and treatment of chronic non-healing skin wounds.","authors":"Giovanni Marfia, Stefania Elena Navone, Clara Di Vito, Nicola Ughi, Silvia Tabano, Monica Miozzo, Carlo Tremolada, Gianni Bolla, Chiara Crotti, Francesca Ingegnoli, Paolo Rampini, Laura Riboni, Roberta Gualtierotti, Rolando Campanella","doi":"10.1080/15476278.2015.1126018","DOIUrl":"10.1080/15476278.2015.1126018","url":null,"abstract":"<p><p>Wound healing is a complex physiological process including overlapping phases (hemostatic/inflammatory, proliferating and remodeling phases). Every alteration in this mechanism might lead to pathological conditions of different medical relevance. Treatments for chronic non-healing wounds are expensive because reiterative treatments are needed. Regenerative medicine and in particular mesenchymal stem cells approach is emerging as new potential clinical application in wound healing. In the past decades, advance in the understanding of molecular mechanisms underlying wound healing process has led to extensive topical administration of growth factors as part of wound care. Currently, no definitive treatment is available and the research on optimal wound care depends upon the efficacy and cost-benefit of emerging therapies. Here we provide an overview on the novel approaches through stem cell therapy to improve cutaneous wound healing, with a focus on diabetic wounds and Systemic Sclerosis-associated ulcers, which are particularly challenging. Current and future treatment approaches are discussed with an emphasis on recent advances. </p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 1","pages":"183-206"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1126018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59981895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 82
Repurposed biological scaffolds: kidney to pancreas. 重新利用的生物支架:肾脏到胰腺。
IF 2.3 4区 生物学
Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1067354
Bradley J Willenberg, Jose Oca-Cossio, Yunqing Cai, Alicia R Brown, William L Clapp, Dale R Abrahamson, Naohiro Terada, Gary W Ellison, Clayton E Mathews, Christopher D Batich, Edward A Ross
{"title":"Repurposed biological scaffolds: kidney to pancreas.","authors":"Bradley J Willenberg,&nbsp;Jose Oca-Cossio,&nbsp;Yunqing Cai,&nbsp;Alicia R Brown,&nbsp;William L Clapp,&nbsp;Dale R Abrahamson,&nbsp;Naohiro Terada,&nbsp;Gary W Ellison,&nbsp;Clayton E Mathews,&nbsp;Christopher D Batich,&nbsp;Edward A Ross","doi":"10.1080/15476278.2015.1067354","DOIUrl":"https://doi.org/10.1080/15476278.2015.1067354","url":null,"abstract":"<p><p>Advances in organ regeneration have been facilitated by gentle decellularization protocols that maintain distinct tissue compartments, and thereby allow seeding of blood vessels with endothelial lineages separate from populations of the parenchyma with tissue-specific cells. We hypothesized that a reconstituted vasculature could serve as a novel platform for perfusing cells derived from a different organ: thus discordance of origin between the vascular and functional cells, leading to a hybrid repurposed organ. The need for a highly vascular bed is highlighted by tissue engineering approaches that involve transplantation of just cells, as attempted for insulin production to treat human diabetes. Those pancreatic islet cells present unique challenges since large numbers are needed to allow the cell-to-cell signaling required for viability and proper function; however, increasing their number is limited by inadequate perfusion and hypoxia. As proof of principle of the repurposed organ methodology we harnessed the vasculature of a kidney scaffold while seeding the collecting system with insulin-producing cells. Pig kidneys were decellularized by sequential detergent, enzymatic and rinsing steps. Maintenance of distinct vascular and collecting system compartments was demonstrated by both fluorescent 10 micron polystyrene microspheres and cell distributions in tissue sections. Sterilized acellular scaffolds underwent seeding separately via the artery (fibroblasts or endothelioma cells) and retrograde (murine βTC-tet cells) up the ureter. After three-day bioreactor incubation, histology confirmed separation of cells in the vasculature from those in the collecting system. βTC-tet clusters survived in tubules, glomerular Bowman's space, demonstrated insulin immunolabeling, and thereby supported the feasibility of kidney-to-pancreas repurposing. </p>","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 2","pages":"47-57"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1067354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33971423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
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