Organogenesis最新文献_第10页

The fatty acid chain elongase, Elovl1, is required for kidney and swim bladder development during zebrafish embryogenesis. 在斑马鱼胚胎发育过程中，脂肪酸链延长酶Elovl1是肾脏和鱼鳔发育所必需的。

IF 2.3 4区生物学

Organogenesis Pub Date : 2016-04-02 Epub Date: 2016-04-14 DOI: 10.1080/15476278.2016.1172164

Sushil Bhandari, Joon No Lee, Young-Il Kim, In-Koo Nam, Su-Jung Kim, Se-Jin Kim, SeongAe Kwak, Gi-Su Oh, Hyung-Jin Kim, Hyun Ju Yoo, Hong-Seob So, Seong-Kyu Choe, Raekil Park

{"title":"The fatty acid chain elongase, Elovl1, is required for kidney and swim bladder development during zebrafish embryogenesis.","authors":"Sushil Bhandari, Joon No Lee, Young-Il Kim, In-Koo Nam, Su-Jung Kim, Se-Jin Kim, SeongAe Kwak, Gi-Su Oh, Hyung-Jin Kim, Hyun Ju Yoo, Hong-Seob So, Seong-Kyu Choe, Raekil Park","doi":"10.1080/15476278.2016.1172164","DOIUrl":"https://doi.org/10.1080/15476278.2016.1172164","url":null,"abstract":"Very long chain fatty acids are required for sphingolipid synthesis, lipid homeostasis, myelin formation, epidermal permeability, and retinal function. Seven different enzymes are known to be involved in the elongation cycle of fatty acids, with different chain-length specificities. Elovl1 is one of those enzymes whose function has been linked mainly to the synthesis of sphingolipids and the epidermal barrier. However, the role of Elovl1 in organogenesis is not clear. In zebrafish, 2 Elovl1 genes, elovl1a and elovl1b, are highly expressed in the swim bladder, and elovl1b is also expressed in the kidney. We found that both elovl1 knockdown embryos contain increased levels of long chain fatty acids from carbon number 14 to 20 as compared to control embryos. Oil-Red-O staining shows that yolk lipid consumption is greatly reduced, whereas lipid droplets accumulate within the swim bladder. Notably, knockdown of either elovl1a or elovl1b affects the expression of genes involved in swim bladder development and impairs inflation of the swim bladder. Consistent with its expression in the pronephros, knockdown of elovl1b alone affects the expression of genes required for kidney development and reduces renal clearance. Our findings strongly suggest that both elovl1 genes are a key determinant of swim bladder and kidney development in zebrafish, which may be comparatively applicable to lung and kidney development in humans.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"12 2","pages":"78-93"},"PeriodicalIF":2.3,"publicationDate":"2016-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2016.1172164","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34403579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Membrane channel gene expression in human costal and articular chondrocytes. 人肋关节软骨细胞膜通道基因的表达。

IF 2.3 4区生物学

Organogenesis Pub Date : 2016-04-02 Epub Date: 2016-04-26 DOI: 10.1080/15476278.2016.1181238

A Asmar, R Barrett-Jolley, A Werner, R Kelly, M Stacey

{"title":"Membrane channel gene expression in human costal and articular chondrocytes.","authors":"A Asmar, R Barrett-Jolley, A Werner, R Kelly, M Stacey","doi":"10.1080/15476278.2016.1181238","DOIUrl":"https://doi.org/10.1080/15476278.2016.1181238","url":null,"abstract":"Chondrocytes are the uniquely resident cells found in all types of cartilage and key to their function is the ability to respond to mechanical loads with changes of metabolic activity. This mechanotransduction property is, in part, mediated through the activity of a range of expressed transmembrane channels; ion channels, gap junction proteins, and porins. Appropriate expression of ion channels has been shown essential for production of extracellular matrix and differential expression of transmembrane channels is correlated to musculoskeletal diseases such as osteoarthritis and Albers-Schönberg. In this study we analyzed the consistency of gene expression between channelomes of chondrocytes from human articular and costal (teenage and fetal origin) cartilages. Notably, we found 14 ion channel genes commonly expressed between articular and both types of costal cartilage chondrocytes. There were several other ion channel genes expressed only in articular (6 genes) or costal chondrocytes (5 genes). Significant differences in expression of BEST1 and KCNJ2 (Kir2.1) were observed between fetal and teenage costal cartilage. Interestingly, the large Ca(2+) activated potassium channel (BKα, or KCNMA1) was very highly expressed in all chondrocytes examined. Expression of the gap junction genes for Panx1, GJA1 (Cx43) and GJC1 (Cx45) was also observed in chondrocytes from all cartilage samples. Together, this data highlights similarities between chondrocyte membrane channel gene expressions in cells derived from different anatomical sites, and may imply that common electrophysiological signaling pathways underlie cellular control. The high expression of a range of mechanically and metabolically sensitive membrane channels suggest that chondrocyte mechanotransduction may be more complex than previously thought.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"12 2","pages":"94-107"},"PeriodicalIF":2.3,"publicationDate":"2016-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2016.1181238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34433890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

New theory of uterovaginal embryogenesis 子宫-阴道胚胎发生的新理论

IF 2.3 4区生物学

Organogenesis Pub Date : 2016-01-02 DOI: 10.1080/15476278.2016.1145317

Z. Makiyan

{"title":"New theory of uterovaginal embryogenesis","authors":"Z. Makiyan","doi":"10.1080/15476278.2016.1145317","DOIUrl":"https://doi.org/10.1080/15476278.2016.1145317","url":null,"abstract":"ABSTRACT Background: The explanation of uterine and vaginal embryogenesis in humans still poses many controversies, because it is difficult to assess early stages of an embryo. The literature review revealed many disagreements in Mullerian theory, inciting some authors to propose new embryological hypotheses. In the original Mullerian theory: the paramesonephral ducts form the Fallopian tubes, uterus and vagina; the mesonephral ducts regress in female embryos. Aims: The aim of this article is to investigate the development of Mullerian ducts in humans, using comparative analysis of fundamental embryological theory and various utero-vaginal anomalies. Material and methods: Between 1998 and 2015, 434 patients with various uterovaginal malformations had been operated on at the Scientific Centre of Obstetrics Gynaecology and Perynatology in Moscow. The anatomies of the uterovaginal malformations in these patients were diagnosed with ultrasound and MRI and then verified during surgical correction by laparoscopy. Results: A systematic comparison of uterovaginal malformations to those in the literature has allowed us to formulate a new theory of embryonic morphogenesis. The new theory is significantly different: ovary, ovarian ligamentum proprium, and ligamentum teres uteri derive from gonadal ridges; Fallopian tubes and vagina completely develop from mesonephral ducts. The uterus develops in the area of intersection between the mesonephral ducts with gonadal ridges by the fusion of the two. Conclusions: The new theory may to induce future embryological studies. The hypothetic possibility that the ovary and endometrium derive from the gonadal ridges could be the key to understanding the enigmatic aetiologies of extragenital and ovarian endometriosis.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"12 1","pages":"33 - 41"},"PeriodicalIF":2.3,"publicationDate":"2016-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2016.1145317","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59981933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Wnt-YAP interactions in the neural fate of human pluripotent stem cells and the implications for neural organoid formation Wnt-YAP在人类多能干细胞神经命运中的相互作用及其对神经类器官形成的影响

IF 2.3 4区生物学

Organogenesis Pub Date : 2016-01-02 DOI: 10.1080/15476278.2016.1140290

Julie Bejoy, Liqing Song, Yan Li

引用次数: 12

The role of endothelial cells on islet function and revascularization after islet transplantation 内皮细胞在胰岛移植后胰岛功能和血运重建中的作用

IF 2.3 4区生物学

Organogenesis Pub Date : 2016-01-02 DOI: 10.1080/15476278.2016.1165378

A. del Toro-Arreola, A. K. Robles-Murillo, A. Daneri-Navarro, J. Rivas-Carrillo

引用次数: 22

Studies of gonadal sex differentiation 性腺性别分化的研究

IF 2.3 4区生物学

Organogenesis Pub Date : 2016-01-02 DOI: 10.1080/15476278.2016.1145318

Z. Makiyan

{"title":"Studies of gonadal sex differentiation","authors":"Z. Makiyan","doi":"10.1080/15476278.2016.1145318","DOIUrl":"https://doi.org/10.1080/15476278.2016.1145318","url":null,"abstract":"ABSTRACT Gonadal differentiation has a determinative influence on sex development in human embryos. Disorders of sexual development (DSD) have been associated with persistent embryonal differentiation stages. Between 1998 and 2015, 139 female patients with various (DSD) underwent operations at the Scientific Center of Obstetrics, Gynaecology and Perynatology in Moscow, Russia. Clinical investigations included karyotyping, ultrasound imaging, hormonal measurement and investigations of gonadal morphology. The male characteristics in the embryo are imposed by testicular hormones. When these are absent or inactive, the fetus may be arrested at between developmental stages, or stay on indifferent stage and become phenotypically female. A systematic analysis of gonadal morphology in DSD patients and a literature review revealed some controversies and led us to formulate a new hypothesis about sex differentiation. Proliferation of the mesonephric system (tubules and corpuscles) in the gonads stimulates the masculinization of gonads to testis. Sustentacular Sertoli cells of the testes are derived from mesonephric excretory tubules, while interstitial Leydig cells are derived from the original mesenchyme of the mesonephros. According of the new hypothesis, the original mesonephric cells (tubules and corpuscles) potentially persist in the ovarian parenchyma. In female gonads, some mesonephric excretory tubules regress and lose the tubular structure, but form ovarian theca interna and externa, becoming analogous to the sustentacular Sertoli cells in the testis. The ovarian interstitial Leydig cells are derived from intertubal mesenchyme of the mesonephros, similar to what occurs in male gonads (testis). Surprisingly, the leading determinative factor in sexual differentiation of the gonads is the mesonephros, represented by the embryonic urinary system.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"8 12 1","pages":"42 - 51"},"PeriodicalIF":2.3,"publicationDate":"2016-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2016.1145318","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59981964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

The role of stem cells in limb regeneration 干细胞在肢体再生中的作用

IF 2.3 4区生物学

Organogenesis Pub Date : 2016-01-02 DOI: 10.1080/15476278.2016.1163463

Elizabeth R. Zielins, R. C. Ransom, Tripp Leavitt, M. Longaker, D. Wan

引用次数: 14

From hacking the human genome to editing organs 从破解人类基因组到编辑器官

IF 2.3 4区生物学

Organogenesis Pub Date : 2015-10-02 DOI: 10.1080/15476278.2015.1120047

Takamasa Tobita, J. Guzman‐Lepe, Alexandra Collin de l'Hortet

引用次数: 5

Cell Therapy Strategies to Combat Immunosenescence 对抗免疫衰老的细胞治疗策略

IF 2.3 4区生物学

Organogenesis Pub Date : 2015-10-02 DOI: 10.1080/15476278.2015.1120046

E. Stahl, B. Brown

引用次数: 55

3D bioprinting: A new insight into the therapeutic strategy of neural tissue regeneration. 生物3D打印:神经组织再生治疗策略的新见解。

IF 2.3 4区生物学

Organogenesis Pub Date : 2015-01-01 DOI: 10.1080/15476278.2015.1123360

Fu-Yu Hsieh, Shan-hui Hsu

{"title":"3D bioprinting: A new insight into the therapeutic strategy of neural tissue regeneration.","authors":"Fu-Yu Hsieh, Shan-hui Hsu","doi":"10.1080/15476278.2015.1123360","DOIUrl":"10.1080/15476278.2015.1123360","url":null,"abstract":"Acute traumatic injuries and chronic degenerative diseases represent the world's largest unmet medical need. There are over 50 million people worldwide suffering from neurodegenerative diseases. However, there are only a few treatment options available for acute traumatic injuries and neurodegenerative diseases. Recently, 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation. In this commentary, the newly developed 3D bioprinting technique involving neural stem cells (NSCs) embedded in the thermoresponsive biodegradable polyurethane (PU) bioink is reviewed. The thermoresponsive and biodegradable PU dispersion can form gel near 37 °C without any crosslinker. NSCs embedded within the water-based PU hydrogel with appropriate stiffness showed comparable viability and differentiation after printing. Moreover, in the zebrafish embryo neural deficit model, injection of the NSC-laden PU hydrogels promoted the repair of damaged CNS. In addition, the function of adult zebrafish with traumatic brain injury was rescued after implantation of the 3D-printed NSC-laden constructs. Therefore, the newly developed 3D bioprinting technique may offer new possibilities for future therapeutic strategy of neural tissue regeneration.","PeriodicalId":19596,"journal":{"name":"Organogenesis","volume":"11 1","pages":"153-8"},"PeriodicalIF":2.3,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15476278.2015.1123360","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59981889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81