Enamel matrix derivative enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells on the titanium implant surface.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2017-07-03 Epub Date: 2017-06-09 DOI:10.1080/15476278.2017.1331196
Guang Li, Jing Hu, Hui Chen, Liang Chen, Na Zhang, Lisheng Zhao, Ning Wen, Yongjin Yang
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引用次数: 23

Abstract

Periodontal ligament stem cells (PDLSCs) have mesenchymal-stem-cells-like qualities, and are considered as one of the candidates of future clinical application in periodontal regeneration therapy. Enamel matrix derivative (EMD) is widely used in promoting periodontal regeneration. However, the effects of EMD on the proliferation and osteogenic differentiation of human PDLSCs grown on the Ti implant surface are still no clear. Therefore, this study examined the effects of EMD on human PDLSCs in vitro. Human PDLSCs were isolated from healthy participants, and seeded on the surface of Ti implant disks and stimulated with various concentrations of EMD. Cell proliferation was determined with Cell Counting Kit-8 (CCK-8). The osteogenic differentiation of PDLSCs was evaluated by the measurement of alkaline phosphatase (ALP) activity, Alizarin red staining, and real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. The results indicated that EMD at concentrations (5-60 µg/ml) increased the viability and proliferation of PDLSCs. The treatment with 30 and 60 µg/ml of EMD significantly elevated ALP activity, augmented mineralized nodule formation and calcium deposition, and upregulated the mRNA and protein levels of Runx-2 and osteocalcin (OCN) in the PDLSCs grown on the Ti surface. Further investigation found that EMD treatment did not change the protein levels of phosphatidylinositol-3-kinase (PI3K), p-PI3K, Akt and mTOR, but significantly upregulated the phosphorylated levels of Akt and mTOR. Collectively, these results suggest that EMD stimulation can promote the proliferation and osteogenic differentiation of PDLSCs grown on Ti surface, which is possibly associated with the activation of Akt/mTOR signaling pathway.

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牙釉质基质衍生物促进人牙周韧带干细胞在钛种植体表面的增殖和成骨分化。
牙周韧带干细胞(Periodontal ligament stem cells, PDLSCs)具有间充质干细胞的特性,被认为是牙周再生治疗中未来临床应用的候选细胞之一。牙釉质基质衍生物(EMD)在促进牙周再生方面有着广泛的应用。然而,EMD对Ti种植体表面生长的人PDLSCs增殖和成骨分化的影响尚不清楚。因此,本研究在体外研究了EMD对人PDLSCs的影响。从健康参与者身上分离出人PDLSCs,将其植入钛植入盘表面,并用不同浓度的EMD刺激。用细胞计数试剂盒-8 (CCK-8)检测细胞增殖。分别采用碱性磷酸酶(ALP)活性测定、茜素红染色、实时聚合酶链反应(qRT-PCR)和Western blotting检测PDLSCs的成骨分化情况。结果表明,EMD浓度(5 ~ 60µg/ml)可提高PDLSCs的活力和增殖能力。30µg/ml和60µg/ml的EMD处理显著提高了钛表面生长的PDLSCs的ALP活性,增强了矿化结节形成和钙沉积,上调了Runx-2和骨钙素(OCN)的mRNA和蛋白水平。进一步研究发现,EMD治疗并未改变磷脂酰肌醇-3激酶(PI3K)、p-PI3K、Akt和mTOR的蛋白水平,但显著上调了Akt和mTOR的磷酸化水平。综上所述,EMD刺激可以促进Ti表面生长的PDLSCs的增殖和成骨分化,这可能与Akt/mTOR信号通路的激活有关。
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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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