Current strategies to generate mature human induced pluripotent stem cells derived cholangiocytes and future applications

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2017-01-02 DOI:10.1080/15476278.2016.1278133

Eduardo Cervantes-Alvarez, Yang Wang, Alexandra Collin de l'Hortet, J. Guzman‐Lepe, Jiye Zhu, K. Takeishi

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引用次数: 8

Abstract

ABSTRACT Stem cell research has significantly evolved over the last few years, allowing the differentiation of pluripotent cells into almost any kind of lineage possible. Studies that focus on the liver have considerably taken a leap into this novel technology, and hepatocyte-like cells are being generated that are close to resembling actual hepatocytes both genotypically and phenotypically. The potential of this extends from disease models to bioengineering, and even also innovative therapies for end-stage liver disease. Nonetheless, too few attention has been given to the non-parenchymal cells which are also fundamental for normal liver function. This includes cholangiocytes, the cells of the biliary epithelium, without whose role in bile modification and metabolism would impair hepatocyte survival. Such can be observed in diseases that target them, so called cholangiopathies, for which there is much yet to study so as to improve therapeutical options. Protocols that describe the induction of human induced pluripotent stem cells into cholangiocytes are scarce, although progress is being achieved in this area as well. In order to give the current view on this emerging research field, and in hopes to motivate further advances, we present here a review on the known differentiation strategies with sight into future applications.

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目前产生成熟人诱导多能干细胞来源的胆管细胞的策略及其未来应用

干细胞研究在过去几年中有了显著的发展，使多能细胞分化成几乎任何种类的谱系成为可能。专注于肝脏的研究已经在这项新技术上取得了很大的飞跃，肝细胞样细胞正在产生，它们在基因和表型上都接近于实际的肝细胞。这种潜力从疾病模型延伸到生物工程，甚至是终末期肝病的创新疗法。然而，对非实质细胞的关注太少，而非实质细胞也是正常肝功能的基础。这包括胆管细胞，胆道上皮细胞，没有它们在胆汁修饰和代谢中的作用将损害肝细胞的存活。这种情况可以在以它们为目标的疾病中观察到，即所谓的胆管病，为了改进治疗方案，还有很多有待研究的地方。描述人类诱导多能干细胞进入胆管细胞的方案很少，尽管在这一领域也取得了进展。为了给出目前对这一新兴研究领域的看法，并希望推动进一步的进展，我们在这里对已知的差异化策略进行了回顾，并展望了未来的应用。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.