Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2018-01-02 Epub Date: 2018-04-27 DOI:10.1080/15476278.2018.1436023
Xiao-Dong Chen, Shu-Bin Ruan, Ze-Peng Lin, Ziheng Zhou, Feng-Gang Zhang, Rong-Hua Yang, Ju-Lin Xie
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引用次数: 8

Abstract

Skin wound healing involves Notch/Jagged1 signaling. However, little is known how Jag1 expression level in epidermal stem cells (ESCs) contributes to wound healing and scar formation. We applied multiple cellular and molecular techniques to examine how Jag1 expression in ESCs modulates ESCs differentiation to myofibroblasts (MFB) in vitro, interpret how Jag1 expression in ESCs is involved in wound healing and scar formation in mice, and evaluate the effects of porcine acellular dermal matrix (ADM) treatment on wound healing and scar formation. We found that Jag1, Notch1 and Hes1 expression was up-regulated in the wound tissue during the period of wound healing. Furthermore, Jag1 expression level in the ESCs was positively associated with the level of differentiation to MFB. ESC-specific knockout of Jag1 delayed wound healing and promoted scar formation in vivo. In addition, we reported that porcine ADM treatment after skin incision could accelerate wound closure and reduce scar formation in vivo. This effect was associated with decreased expression of MFB markers, including α-SMA Col-1 and Col-III in wound tissues. Finally, we confirmed that porcine ADM treatment could increase Jag1, Notch1 and Hesl expression in wound tissues. Taken together, our results suggested that ESC-specific Jag1 expression levels are critical for wound healing and scar formation, and porcine ADM treatment would be beneficial in promoting wound healing and preventing scar formation by enhancing Notch/Jagged1 signaling pathway in ESCs.

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猪脱细胞真皮基质处理对伤口愈合和瘢痕形成的影响:表皮干细胞中Jag1表达的作用
皮肤伤口愈合涉及Notch/Jagged1信号。然而,Jag1在表皮干细胞(ESCs)中的表达水平对伤口愈合和疤痕形成的影响尚不清楚。我们应用多种细胞和分子技术,在体外研究了Jag1在ESCs中的表达如何调节ESCs向肌成纤维细胞(MFB)的分化,解释了Jag1在ESCs中的表达如何参与小鼠伤口愈合和疤痕形成,并评估了猪脱细胞真皮基质(ADM)处理对伤口愈合和疤痕形成的影响。我们发现,在创面愈合期间,Jag1、Notch1和Hes1在创面组织中的表达上调。此外,Jag1在ESCs中的表达水平与向MFB的分化水平呈正相关。在体内,esc特异性敲除Jag1会延迟伤口愈合并促进疤痕形成。此外,我们报道了猪皮肤切口后的ADM处理可以加速伤口愈合,减少体内疤痕的形成。这种作用与伤口组织中MFB标志物(包括α-SMA Col-1和Col-III)的表达降低有关。最后,我们证实了ADM处理可以提高猪伤口组织中Jag1、Notch1和Hesl的表达。综上所述,我们的研究结果表明,esc特异性Jag1表达水平对伤口愈合和疤痕形成至关重要,猪ADM处理可能通过增强ESCs中Notch/Jagged1信号通路,促进伤口愈合和防止疤痕形成。
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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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