Anatomical structure, and expression of CCL4 and CCL13-like during the development of maxillary barbel in Paramisgurnus dabryanus.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2019-01-01 Epub Date: 2019-07-06 DOI:10.1080/15476278.2019.1633870

Kianann Tan, Ruijing Geng, Zhiqiang Wang, Han Liu, Weimin Wang

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Abstract

Paramisgurnus dabryanus is one of the most economically important fishes in China. Barbels are an essential sensory organ for the food-seeking ability of teleost fish. However, the anatomical structure of the maxillary barbels of P. dabryanus and the molecular basis of their development are unknown. We investigated the anatomical structure of the barbel, and gene expression patterns of two chemokine C-C motif ligands: CCL4 and CCL13-like during the maxillary barbel development using Masson Trichrome staining, light and electron microscopy, and qPCR. Anatomically, the maxillary barbel of P. dabryanus contains taste buds, melanophores, collagen fibers, connective tissue, smooth muscles, nerve bundles, and blood vessels, but does not have skeletal muscles or a skeleton rod. The expression of CCL4 and CCL13-like was weak or non-existent in the early phases of development, but high at the last two studied time-points: 192- and 216-h post-hatching. Results indicated that CCL4 and CCL13-like were related to the development of the maxillary barbel.

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大鳞副龙上颌倒钩发育过程中CCL4和ccl13样蛋白的表达及解剖结构。

dabryanparamisgurus是中国最具经济价值的鱼类之一。硬骨鱼的鱼刺是其觅食能力的重要感觉器官。然而，颌刺的解剖结构及其发育的分子基础尚不清楚。我们利用马松三色染色、光镜、电镜和qPCR技术研究了上颌倒刺发育过程中两种趋化因子C-C基序配体CCL4和ccl13 -样的基因表达模式。解剖学上，P. dabryanus的上颌须含有味蕾、黑色素细胞、胶原纤维、结缔组织、平滑肌、神经束和血管，但没有骨骼肌或骨棒。CCL4和CCL13-like在早期发育阶段表达较弱或不存在，但在孵化后192和216 h的最后两个研究时间点表达较高。结果表明，CCL4和ccl13 -样蛋白与上颌须的发育有关。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.