Nucleosides, Nucleotides & Nucleic Acids最新文献_第4页

Identification of key genes in the liver of offspring from obesity maternal mice by bioinformatics analysis. 利用生物信息学分析鉴定肥胖母鼠后代肝脏关键基因。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-13 DOI: 10.1080/15257770.2025.2517378

Qiu-Tong Chen, Ming-Wei Liu, Hong-Jie Yu, Jie Zhang, Qi-Qiang He

{"title":"Identification of key genes in the liver of offspring from obesity maternal mice by bioinformatics analysis.","authors":"Qiu-Tong Chen, Ming-Wei Liu, Hong-Jie Yu, Jie Zhang, Qi-Qiang He","doi":"10.1080/15257770.2025.2517378","DOIUrl":"https://doi.org/10.1080/15257770.2025.2517378","url":null,"abstract":"Murine maternal obesity predisposes offspring to obesity and other non-communicable diseases. Fetal programming enables researchers to trace this detrimental effect in the early life of the offspring. The aim of the current study was to explore the molecular impact of maternal obesity on the livers of murine offspring at weaning age. C57BL/6 female mice were exposed to a high-fat diet to induce obesity, after which they were mated to produce offspring. At weaning age, the metabolic health of female offspring was assessed and hepatic mRNAs were investigated via mRNA high throughput sequencing. The differentially expressed genes were identified using gene and protein expression analyses. The results revealed that murine maternal obesity altered the blood parameters, liver histology and gene expression of offspring. Cyclin-dependent kinase 1 (Cdk1) and E2f transcription factor 1 (E2f1) were identified as hub genes by bioinformatics analysis. Reverse transcription-quantitative PCR, western blotting and immunohistochemical analysis all revealed that the expression of Cdk1 and E2f1 was decreased in the livers of offspring born from obese does. In conclusion, murine maternal obesity impaired lipid metabolism in the livers of their offspring at weaning age. Furthermore, Cdk1 and E2f1 were identified as hub genes in the regulatory mechanism.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-17"},"PeriodicalIF":1.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A positive correlation between the pseudorotational phase angle P and the δ_H torsion angle (H4'-C4'-C3'-H3') in nucleosides and nucleic acids. 核苷和核酸的赝旋相角P与δH扭角（H4′-C4′-C3′-H3′）呈正相关。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-11 DOI: 10.1080/15257770.2025.2516597

Jesse Vanloon, Alexander Y Yan, Hongbin Yan

引用次数: 0

Co-regulation of miRNA and lncRNA on immunosuppression gene: unveiling the regulatory networks in cancer. miRNA和lncRNA对免疫抑制基因的共同调控：揭示肿瘤中的调控网络。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-06 DOI: 10.1080/15257770.2025.2514129

A G Dharini, Priyatharcini Kejamurthy, K T Ramya Devi

{"title":"Co-regulation of miRNA and lncRNA on immunosuppression gene: unveiling the regulatory networks in cancer.","authors":"A G Dharini, Priyatharcini Kejamurthy, K T Ramya Devi","doi":"10.1080/15257770.2025.2514129","DOIUrl":"https://doi.org/10.1080/15257770.2025.2514129","url":null,"abstract":"Cancer cells often evade immune detection and destruction by inducing immune suppression genes, which include CTLA-4, TGF-β, and PD-L1, that inhibit immune responses and promote tumour progression. Recent studies have highlighted the significance of non-coding RNAs, particularly microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), in regulating these immune suppression pathways. miRNAs, short RNA molecules that target mRNA of immune genes at the post-transcription level and influence gene expression. Similarly, lncRNAs, which act as molecular scaffolds, sponges, or regulators of gene expression, are involved in modulating immune responses by interacting with miRNAs or directly binding to immune-related genes. This review explores the complex interplay between miRNAs, lncRNAs, and immune suppression genes, detailing how these non-coding RNAs contribute to immune evasion in cancer. Furthermore, the therapeutic potential of targeting these regulatory networks is examined, highlighting current strategies and challenges in using miRNA and lncRNA modulators to enhance anti-tumour immunity. Understanding these intricate regulatory networks offers new insights into the mechanisms of immune suppression in cancer and opens avenues for developing novel therapeutic interventions to restore immune surveillance and improve the efficacy of cancer immunotherapies.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-30"},"PeriodicalIF":1.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamers as therapeutic targets: prospects and progress in the treatment of cancers. 适体作为治疗靶点：癌症治疗的前景与进展。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-06 DOI: 10.1080/15257770.2025.2512853

Yash Sansare, Priyatharcini Kejamurthy, Suramya Singh, Aryan Ayush, Koustubhi Khani, K T Ramya Devi

{"title":"Aptamers as therapeutic targets: prospects and progress in the treatment of cancers.","authors":"Yash Sansare, Priyatharcini Kejamurthy, Suramya Singh, Aryan Ayush, Koustubhi Khani, K T Ramya Devi","doi":"10.1080/15257770.2025.2512853","DOIUrl":"https://doi.org/10.1080/15257770.2025.2512853","url":null,"abstract":"Contemporary cancer treatments encompass diverse strategies like surgery, chemotherapy, radiation, immunotherapy, and targeted therapies, aiming for effective cancer cell control with minimal impact on healthy tissues. Aptamers are short nucleotide sequences typically containing 25-80 bases and can attach to specific target molecules as effectively as monoclonal antibodies. While the FDA has yet to approve any aptamers for oncology applications, a few, such as Pegaptanib (Macugen), have been approved for ophthalmologic conditions like age-related macular degeneration. Pegaptanib and Izervay are the approved aptamers against age-related macular degeneration (AMD) that target vascular endothelial growth factor (VEGF) and block complement component protein C5, respectively. A new type of highly sensitive and specific biosensor has recently been created to detect leukaemia cancer cells. Aptamosomes, encapsulating drugs like doxorubicin, effectively reduce tumour size and are highly advantageous over targeted drug delivery. Many aptamers have been generated against ERα, Epithelial cell adhesion molecule, EGFR, B subunit of platelet-derived growth factor, Vimentin, Osteopontin, Type II membrane protein PSMA, MUC-1, AXL receptor tyrosine kinase, CD28 agonistic aptamer, as well as for the B7-CD28 interaction, etc. This review suggests the pros and cons of aptamer usage and its advantages over antibody treatment. It also outlines the roles of aptamers and connects their modes of action with specific cancer types. The content is highly detailed, providing a comprehensive understanding of aptamer therapy and its applications.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-39"},"PeriodicalIF":1.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the interactions of Axitinib, a tyrosine kinase inhibitor, with DNA: experimental studies, molecular docking, and molecular dynamics simulations. 研究酪氨酸激酶抑制剂阿西替尼与DNA的相互作用：实验研究、分子对接和分子动力学模拟。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-02 DOI: 10.1080/15257770.2025.2509977

Pelin Şenel, Abdullah Al Faysal, Soykan Agar, Mine Yurtsever, Ayşegül Gölcü

{"title":"Investigating the interactions of Axitinib, a tyrosine kinase inhibitor, with DNA: experimental studies, molecular docking, and molecular dynamics simulations.","authors":"Pelin Şenel, Abdullah Al Faysal, Soykan Agar, Mine Yurtsever, Ayşegül Gölcü","doi":"10.1080/15257770.2025.2509977","DOIUrl":"https://doi.org/10.1080/15257770.2025.2509977","url":null,"abstract":"Axitinib is an oral medication classified as a second-generation tyrosine kinase inhibitor. It serves as a primary treatment for metastatic renal cell carcinoma (RCC) due to its strong affinity for DNA, which leads to the disruption of the double helix structure. This disruption ultimately halts the cell cycle and induces senescence and mitotic catastrophe in RCC cells. Consequently, investigating the mechanism by which Axitinib binds to DNA is essential for comprehending its pharmacodynamic properties and for the advancement of more effective DNA-binding therapeutics. The present study aimed to examine the interaction between Axitinib and DNA through various analytical techniques, including UV-Vis spectroscopy, thermal denaturation assays, electrochemical methods, and fluorescence emission spectroscopy. According to the electrochemical studies, the binding constant (Kb) for Axitinib was calculated to be (5.13 ± 0.28) × 104, suggesting the potential for groove binding. This finding was further supported by in-silico analyses, where molecular docking and molecular dynamics simulations indicated that the drug selectively binds to the DNA minor groove through partial intercalation, forming new hydrogen bonds with its functional groups while separating the guanine and cytosine base pairs.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-24"},"PeriodicalIF":1.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical and electrochemical studies on the interaction of arbutin drug with calf-thymus DNA. 熊果苷类药物与小牛胸腺DNA相互作用的生物物理和电化学研究。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-02 DOI: 10.1080/15257770.2025.2512857

D S Bhuvaneshwari, Kandasamy Pavithra, Kuppanagounder P Elango

{"title":"Biophysical and electrochemical studies on the interaction of arbutin drug with calf-thymus DNA.","authors":"D S Bhuvaneshwari, Kandasamy Pavithra, Kuppanagounder P Elango","doi":"10.1080/15257770.2025.2512857","DOIUrl":"https://doi.org/10.1080/15257770.2025.2512857","url":null,"abstract":"Understanding the interaction of therapeutic drugs with DNA is crucial for designing highly selective DNA-targeted medicines that could overcome the current therapeutic limitations. In this endeavour, the DNA binding behaviour of arbutin (ATN) was explored using multi-spectroscopic, electrochemical and computational studies. The UV-Vis spectral studies authenticated the complexation of ATN with CT-DNA and exposed ATN as a moderately strong DNA binder with a binding constant of 8.029 × 103 M-1. The findings of fluorescence spectral studies not only revealed the spontaneous ground state complex formation between ATN and CT-DNA, but also emphasised the role of hydrogen bonding and Van der Waals interactions in stabilising the ATN/CT-DNA complex. Since the competitive dye displacement assay strongly excluded the plausibility of classical intercalation and conventional groove binding mode of ATN, viscosity studies provided clues regarding the external binding mode of ATN. The appreciable enhancement resulted in the fluorescence emission of the ATN/CT-DNA complex upon increasing NaCl concentration, which certified ATN as an external binder. The CD spectral results exposed the ATN-induced moderate conformational alterations in CT-DNA. Remarkably, the voltammetric titration results labelled the glucopyranoside moiety of ATN as a DNA binding unit with a formation constant of 2.57 × 104 M-1 rather than the hydroquinone moiety of ATN. Molecular docking and metadynamics simulation outcomes served as pictorial evidence of experimental results. They revealed the predominant contribution of hydrogen bonding interactions in stabilising ATN/DNA complexation.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-23"},"PeriodicalIF":1.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-34a rs2666433 and cognitive function in major depressive disorder: a clinical correlation analysis. MiR-34a rs2666433与重度抑郁症认知功能的临床相关性分析

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-02 DOI: 10.1080/15257770.2025.2511106

Ning Li, Xiaochuan Zhao

{"title":"MiR-34a rs2666433 and cognitive function in major depressive disorder: a clinical correlation analysis.","authors":"Ning Li, Xiaochuan Zhao","doi":"10.1080/15257770.2025.2511106","DOIUrl":"https://doi.org/10.1080/15257770.2025.2511106","url":null,"abstract":"Background: Genetic factors play a crucial role in the development of major depressive disorder (MDD).Objectives: This study aimed to investigate the association between the microRNA (miR)-34a rs266643 polymorphism and MDD, as well as its impact on cognitive function.Materials and methods: Clinical data and blood samples were collected from 302 MDD patients and 306 healthy controls who met the predefined inclusion and exclusion criteria. The severity of MDD in patients was assessed using the Hamilton Rating Scale for Depression (HRSD). Cognitive function in MDD patients was evaluated using the Mini-Mental State Examination (MMSE), Stroop Color-Word Test (Stroop-C and Stroop-CW), and Montreal Cognitive Assessment (MoCA). Gene typing was performed using the Sanger sequencing method, while the relative expression level of miR-34a was quantified by RT-qPCR.Results: The MDD group exhibited a significantly higher miR-34a expression fold change compared to the control group (p < 0.001). Among the genotypes, the AA genotype demonstrated the highest expression, followed by GA, with both being significantly greater than GG. The expression of miR-34a was positively correlated with HRSD, Stroop-C, and Stroop-CW scores but negatively correlated with MMSE and MoCA scores (p < 0.001). Carrying the A allele (OR = 1.468, p = 0.002) or the AA genotype (OR = 2.382, p = 0.001) was associated with an increased risk of MDD. Furthermore, patients with the AA genotype exhibited significantly poorer cognitive function compared to other genotypes.Conclusion: The gene polymorphism of miR-34a rs2666433 was significantly associated with the severity of MDD as well as cognitive function.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the expression levels of MEFV gene in patients with frequent MEFV pathogenic variants in Kahramanmaras (Turkey). 土耳其Kahramanmaras地区MEFV致病性变异体患者MEFV基因表达水平的研究

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-05-28 DOI: 10.1080/15257770.2025.2511104

Eda Ganiyusufoglu, Hasan Daglı, Metin Kılınc

{"title":"Investigation of the expression levels of MEFV gene in patients with frequent MEFV pathogenic variants in Kahramanmaras (Turkey).","authors":"Eda Ganiyusufoglu, Hasan Daglı, Metin Kılınc","doi":"10.1080/15257770.2025.2511104","DOIUrl":"10.1080/15257770.2025.2511104","url":null,"abstract":"The main objective of this study is to detect the variants in patients who were diagnosed with familial Mediterranean fever (FMF) according to Tel-Hashomer diagnostic criteria and investigated the relationship between genotype-phenotype and the gene expression levels of the Mediterranean fever (MEFV) gene. Variant screening was achieved by automated sanger sequencing, and expression levels of the MEFV gene were analyzed by quantitative real time polymerase chain reaction (RT-PCR). A total of 46 patients with MEFV gene pathogenic variants and 8 control individuals without any variants were enrolled in the study. The most commonly encountered variants in heterozygote genotype were M694V (n = 4), E148Q (n = 3), and M680I (n = 2); in compound heterozygote genotype were M694V/R202Q (n = 4), and R202Q/E148Q (n = 3); in complex heterozygote genotype were R202Q/M694V/M680I (n = 4) and R202Q/M694V/V726A (n = 3); in homozygote genotype were M680I/M680I (n = 7) and M694V/M694V (n = 4). The gene expression levels of the patients with homozygous variants were found to be significantly lower than the healthy control group and patients with heterozygous variants. In patients with M694V homozygous variants, where clinical manifestations are severe, a remarkable decrease in the gene expression of the MEFV gene was observed. It was detected that there was a relationship between the genotype and gene expression level and that the level of gene expression and clinical symptoms were inversely correlated in patients with FMF.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-508-5p regulates macrophage polarization via targeting TSGA10 to promote malignant behavior in esophageal cancer cells. miR-508-5p通过靶向TSGA10调控巨噬细胞极化，促进食管癌细胞的恶性行为。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-05-11 DOI: 10.1080/15257770.2025.2491561

Yuan Zhu, Zuojun Fu, Tianjiao Duan, Jing Wang, Lingjuan Zhang, Guisheng Liu, Xueyan Guo, Rong Zhang

{"title":"miR-508-5p regulates macrophage polarization via targeting TSGA10 to promote malignant behavior in esophageal cancer cells.","authors":"Yuan Zhu, Zuojun Fu, Tianjiao Duan, Jing Wang, Lingjuan Zhang, Guisheng Liu, Xueyan Guo, Rong Zhang","doi":"10.1080/15257770.2025.2491561","DOIUrl":"https://doi.org/10.1080/15257770.2025.2491561","url":null,"abstract":"Background: Esophageal cancer (EC) is among the deadliest malignancies in humans, with various miRNAs shown to regulate its progression by targeting distinct genes. miR-508-5p was identified as being linked to the malignant behavior of various tumors. Nevertheless, the precise role and mechanism of miR-508-5p in esophageal cancer (EC) remain ambiguous.Objective: This investigation focuses on the role and mechanism of the miR-508-5p/TSGA10 axis in the progression of EC.Methods: The expression of miR-508-5p and TSGA10 in EC cell lines was evaluated using quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Cell transfection techniques were used to knock down miR-508-5p and observe its effects on cell proliferation, migration, invasion, and apoptosis. A dual-luciferase reporter gene assay was conducted to verify the targeting relationship of miR-508-5p with TSGA10. Co-culture studies were undertaken to examine the regulatory effect of the miR-508-5p/TSGA10 axis on the polarization state of tumor-associated macrophages (TAMs) and the malignant behavior of EC cells.Results: The expression of miR-508-5p was significantly elevated in EC cells. Knocking down miR-508-5p curbed cell proliferation, migration, and invasion while promoting apoptosis. TSGA10 was validated as a primary target gene of miR-508-5p. miR-508-5p knockdown could inhibit the M2 polarization of TAMs by upregulating TSGA10, thereby suppressing the tumorigenic behavior of EC cells.Conclusion: miR-508-5p promotes the M2 polarization of TAMs and enhances the malignant behavior of EC cells by inhibiting TSGA10.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-15"},"PeriodicalIF":1.1,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binding characterization of small protein-conjugated ssDNA aptamer to recombinant human ICAM-1. 小蛋白偶联的ssDNA适体与重组人ICAM-1的结合特性。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-05-07 DOI: 10.1080/15257770.2025.2500049

Nik Abdul Aziz Nik Kamarudin, Nurfadhlina Musa, Nur Fatihah Mohd Zaidi, Basyirah Ghazali, Mariana Ahamad, Satvinder S Dhaliwal, Khairul Mohd Fadzli Mustaffa

{"title":"Binding characterization of small protein-conjugated ssDNA aptamer to recombinant human ICAM-1.","authors":"Nik Abdul Aziz Nik Kamarudin, Nurfadhlina Musa, Nur Fatihah Mohd Zaidi, Basyirah Ghazali, Mariana Ahamad, Satvinder S Dhaliwal, Khairul Mohd Fadzli Mustaffa","doi":"10.1080/15257770.2025.2500049","DOIUrl":"10.1080/15257770.2025.2500049","url":null,"abstract":"This study investigates the potential of a protein-DNA aptamer conjugate to enhance aptamer binding to recombinant human intercellular adhesion molecule 1 (rhICAM-1). Aptamers are single-stranded nucleic acids that bind target molecules through hydrogen bonding and hydrophobic interactions. Conjugating aptamers with antibodies or proteins has been shown to improve their binding affinity. Using Systematic Evolution of Ligands by Exponential Enrichment (SELEX), eight rounds of selection were performed with ICAM-1-coupled Dynabeads Protein A, identifying a DI05 as having the strongest binding affinity to rhICAM-1. An antibody inhibition assay showed a significant reduction in rhICAM-1 binding to immobilized aptamers (DI05, DI20, DI31, and DI33). Additionally, the binding affinity of eGFP-conjugated DI05 to rhICAM-1 was higher than that of unconjugated DI05. Docking simulations revealed close contact between DI05 and ICAM-1, with interactions primarily mediated by hydrogen bonds within three hairpin structures at ≤2.8 Å. These findings highlight the potential of aptamer-small protein conjugates as a promising strategy to enhance aptamer binding characteristics.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-23"},"PeriodicalIF":1.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0