Nucleosides, Nucleotides & Nucleic Acids最新文献

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LncRNA PCED1B-AS1 facilitates cervical cancer progression via targeting miR-361-3p. LncRNA PCED1B-AS1通过靶向miR-361-3p促进宫颈癌进展。
IF 1.3 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-08-01 DOI: 10.1080/15257770.2025.2537150
Shuyu Wang, Junwei Wang, Ke Wang
{"title":"LncRNA PCED1B-AS1 facilitates cervical cancer progression via targeting miR-361-3p.","authors":"Shuyu Wang, Junwei Wang, Ke Wang","doi":"10.1080/15257770.2025.2537150","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537150","url":null,"abstract":"<p><p>In recent years, the incidence of cervical cancer has been on the rise, making it imperative to search for targeted therapeutic tumor-associated biomarkers. PCED1B-AS1 regulates gene expression and affects cell proliferation and differentiation in tumors. However, its function and potential mechanisms in cervical cancer remain unclear. The expression of PCED1B-AS1 in cervical cancer and adjacent tissues was detected by qRT-PCR, and its clinical significance was analyzed by chi-square test and Cox model. The interaction between PCED1B-AS1 and miR-361-3p was verified by ENCORI database, and luciferase reporter assay was used to verify the interaction. The CCK-8 and Transwell assays were used to evaluate their effects on cervical cancer cell function. The expression of PCED1B-AS1 in cervical cancer tissues was significantly higher than that in adjacent tissues, and was significantly correlated with tumor grade, stage, and FIGO classification. The upregulation of PCED1B-AS1 can predict adverse prognosis in cervical cancer patients, and the expression of PCED1B-AS1 is negatively correlated with the expression of miR-361-3p. Silencing PCED1B-AS1 significantly inhibited the growth, migration, and invasion of CaSki and HeLa cells, while inhibition of miR-361-3p could diminish this effect. PCED1B-AS1 is significantly upregulated in cervical cancer tissue, and the regulatory axis formed with miR-361-3p may be involved in tumor progression. Importantly, the high expression of PCED1B-AS1 is significantly associated with poor prognosis in patients, suggesting that it not only serves as a novel molecular biomarker for the diagnosis of cervical cancer but may also provide potential intervention targets for the development of targeted therapeutic strategies.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulation of prognosis and tumor progression in cervical cancer by circ_0005728. circ_0005728对宫颈癌预后和肿瘤进展的调节作用。
IF 1.3 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-30 DOI: 10.1080/15257770.2025.2537168
Shanshan Zhang, Xiaoting Shen, Hongyin Cui
{"title":"Regulation of prognosis and tumor progression in cervical cancer by circ_0005728.","authors":"Shanshan Zhang, Xiaoting Shen, Hongyin Cui","doi":"10.1080/15257770.2025.2537168","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537168","url":null,"abstract":"<p><p>To elucidate the prognostic significance and the underlying mechanism of action of circ_0005728 in patients diagnosed with cervical cancer (CCA). Intraoperative CCA and adjacent precancerous tissues from 208 patients were meticulously preserved at -80 °C. Kaplan-Meier survival curves were generated during a comprehensive 5-year postoperative follow-up. Cox analyses predicted the factors influencing prognostic progression in CCA patients. RT-qPCR detected the expression of circ_0005728. CCK8 and transwell observed cell proliferation, migration, and invasion. Flow cytometry recorded apoptotic changes. Dual luciferase reporter assay and RNA precipitation verified the interactions between circ_0005728 and miR-370-3p. High levels of circ_0005728 were observed in CCA tissues and cells. Elevated circ_0005728 expression and lymph node metastasis emerged as independent prognostic factors associated with poor outcomes in CCA patients. After silencing circ_0005728, cell functions were diminished and apoptosis increased. In addition, miR-370-3p is a downstream target gene of circ_0005728. Low expression of miR-370-3p was present in CCA tissues and was negatively correlated with circ_0005728 expression. The use of miR-370-3p inhibitor was able to induce cell proliferation, reduce apoptosis, and resist the reduction of cell migration and invasion caused by transfection of si-circ_0005728. The elevated expression of circ_0005728 in CCA patients was associated with prognostic mortality outcomes. circ_0005728 exerts its influence by targeting miR-370-3p, thereby enhancing the functional capabilities of CCA cells, diminishing apoptosis, and facilitating the malignant progression of CCA.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-20"},"PeriodicalIF":1.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144743356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
miR-34c-3p targets FOXO3 to promote pancreatic carcinoma progression. miR-34c-3p靶向FOXO3促进胰腺癌进展。
IF 1.3 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-28 DOI: 10.1080/15257770.2025.2537167
Zhenxiong Xie, Lin Ji, Fang Wu, Hui Cao
{"title":"miR-34c-3p targets FOXO3 to promote pancreatic carcinoma progression.","authors":"Zhenxiong Xie, Lin Ji, Fang Wu, Hui Cao","doi":"10.1080/15257770.2025.2537167","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537167","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) have been a popular subject of tumor research including pancreatic carcinoma (PC). MicroRNA-34c-3p (miR-34c-3p) is a member of miR-34c cluster, which is strongly associated with tumorigenesis. Nonetheless, miR-34c-3p has not been explored in PC. MiR-34c-3p was taken as a target to explore its current clinical significance and related molecular mechanisms in PC. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to monitor miR-34c-3p and FOXO3 level in tissues and PC cells. Dual-Luciferase reporter assay was utilized to verifying the relationship between miR-34c-3p and FOXO3. Cell Counting Kit-8 (CCK-8) and Transwell assay were applied to detect cell proliferation, migration and invasion. MiR-34c-3p was markedly elevated in PC tissues and closely related with lymph node metastasis, post-treatment nodal margin category and degree of differentiation. MiR-34c-3p upregulation could predict poorer prognosis and higher risk of PC patients. In PC cells, overexpression of miR-34c-3p enhanced cell proliferation, migration and invasion. Moreover, miR-34c-3p negatively regulated FOXO3 to promote cellular processes. High level of miR-34c-3p is a poor prognostic factor for PC patients and miR-34c-3p promotes tumor progression by negatively regulating FOXO3.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering the structural dynamics of Argonaute (AGO)-mediated gene silencing of miR-21-5p, miR-221-3p, miR-126-3p, miR-34a-5p and their role as potential biomarkers in lung cancer. 破译Argonaute (AGO)介导的miR-21-5p、miR-221-3p、miR-126-3p、miR-34a-5p基因沉默的结构动力学及其作为肺癌潜在生物标志物的作用。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-24 DOI: 10.1080/15257770.2025.2535749
Anmol Bhatia, Atul Kumar Upadhyay, Siddharth Sharma
{"title":"Deciphering the structural dynamics of Argonaute (AGO)-mediated gene silencing of miR-21-5p, miR-221-3p, miR-126-3p, miR-34a-5p and their role as potential biomarkers in lung cancer.","authors":"Anmol Bhatia, Atul Kumar Upadhyay, Siddharth Sharma","doi":"10.1080/15257770.2025.2535749","DOIUrl":"https://doi.org/10.1080/15257770.2025.2535749","url":null,"abstract":"<p><p>Lung Cancer is the leading cause of cancer-related deaths worldwide, with over 85% of the cases being of non-small cell lung cancer. Despite the recent advances, lung cancer remains undiagnosed until after the disease has advanced. The role of miRNAs in gene silencing is driven by RNA-induced silencing complex (RISC) consisting of Argonaute (AGO) protein. Understanding the miRNA-assisted gene regulation in lung cancer poses a prospect for an improved diagnostic and preventive measure towards the disease. This study explores miRNA interactions and their target genes in lung cancer, identifying four key miRNAs: miR-21-5p, miR-221-3p, miR-126-3p, and miR-34a-5p. These were shortlisted through their minimum free energy score, pattern conservation, functional, and network analysis. The AGO protein was retrieved, prepared for docking analysis with miRNA-mRNA duplexes, and docked using the HDOCK webserver. The docking results pointed towards the strong binding affinity of the miRNAs towards their targets and the AGO protein playing a crucial role as a driving force for gene expression. Furthermore, the miRNAs were established for their clinical relevance, particularly noting the association of high miR-21-5p expression with poor overall survival, suggesting potential avenues for further molecular investigation in lung cancer development.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-34"},"PeriodicalIF":1.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
miR-766-3p promotes non-small cell lung cancer development through suppression of NR3C2. miR-766-3p通过抑制NR3C2促进非小细胞肺癌的发展。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-20 DOI: 10.1080/15257770.2025.2533391
Xuexi Zhang, Lei Cao, Gang Zhang
{"title":"miR-766-3p promotes non-small cell lung cancer development through suppression of NR3C2.","authors":"Xuexi Zhang, Lei Cao, Gang Zhang","doi":"10.1080/15257770.2025.2533391","DOIUrl":"https://doi.org/10.1080/15257770.2025.2533391","url":null,"abstract":"<p><p>The incidence of non-small cell lung cancer (NSCLC) has exhibited an elevated trend yearly, seriously threatening human health. However, its molecular mechanism is still unknown. The objective of this experiment was to investigate the expression and prognostic value of miR-766-3p in the tissues of NSCLC patients, as well as to provide possible targets for the healing of NSCLC. In this study, miR-766-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2) were detected in tissues of NSCLC patients using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Survival analysis was estimated with Kaplan-Meier and Cox proportional hazards model. <i>In vitro</i> experiments included Cell Counting Kit-8 (CCK-8) viability assay, flow cytometry for apoptosis, Transwell assay for migration and invasion, and luciferase reporter assay for target genes. miR-766-3p levels were clearly elevated in tumor tissues, and high miR-766-3p levels were considered to be a poor prognostic factor. NR3C2 was clearly down-regulated in the serum of NSCLC patients. miR-766-3p overexpression stimulated the proliferation and enhanced metastatic spread of lung cancer cells, whereas down-regulation of NR3C2 reversed the effect of miR-766-3p on cellular activity. miR-766-3p promotes NSCLC development by inhibiting NR3C2 levels and is a potential biomarker. Furthermore, high levels of miR-766-3p are likely to predict poor prognosis in NSCLC.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-11"},"PeriodicalIF":1.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The construction and analysis of a prognostic model based on CD8+ T cell immune-related genes in hepatocellular carcinoma. 基于CD8+ T细胞免疫相关基因的肝癌预后模型的构建与分析。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-18 DOI: 10.1080/15257770.2025.2531134
Zechang Zhang, Yuanyuan Yang, Yujia Zhang, Jing Wang, Huaifang Cai, Lei Yang, Wenxuan Liu
{"title":"The construction and analysis of a prognostic model based on CD8<sup>+</sup> T cell immune-related genes in hepatocellular carcinoma.","authors":"Zechang Zhang, Yuanyuan Yang, Yujia Zhang, Jing Wang, Huaifang Cai, Lei Yang, Wenxuan Liu","doi":"10.1080/15257770.2025.2531134","DOIUrl":"https://doi.org/10.1080/15257770.2025.2531134","url":null,"abstract":"<p><p>The effector functions of CD8<sup>+</sup> T cell significantly influence the immunosuppressive microenvironment in hepatocellular carcinoma (HCC), which is intricately associated with HCC prognosis. Nevertheless, a comprehensive investigation into the relationship between CD8<sup>+</sup> T cell immune-related genes and HCC prognosis remains lacking. This study aimed to construct a prognostic model for HCC using CD8<sup>+</sup> T cell immune-related genes to provide insights for clinical management and prognosis. A prognostic model was constructed by incorporating 16 CD8<sup>+</sup> T cell-specific immune-related genes, yielding area under the curve (AUC) values of 0.821, 0.796, and 0.784 for the prediction of 1-year, 3-year, and 5-year survival, respectively, <i>via</i> receiver operating characteristic (ROC) curve analysis. The qRT-PCR results showed that the mRNA levels of PTMA, RAC1, HSPD1, HSP90AA1, and TANK were significantly higher in HCC cells compared to normal cells (<i>p</i> < 0.05). Further analysis focused on the TANK gene, which was significantly upregulated in HCC tissues (<i>p</i> < 0.05). The CCK-8 and wound healing assays revealed a significant decrease in both the cell proliferation rate and wound-healing rate in the TANK-deficient group compared with the control group (<i>p</i> < 0.05). These findings may offer new insights into the clinical treatment and prognostic evaluation of HCC.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-22"},"PeriodicalIF":1.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of single strand breaks on the formation of DNA interstrand crosslinks induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine. 单链断裂对主要氧化腺嘌呤损伤诱导的DNA链间交联形成的影响7,8-二氢-8-氧腺嘌呤。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-12 DOI: 10.1080/15257770.2025.2529353
Aaron L Rozelle, Louis B Zhang, Seongmin Lee
{"title":"Effect of single strand breaks on the formation of DNA interstrand crosslinks induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine.","authors":"Aaron L Rozelle, Louis B Zhang, Seongmin Lee","doi":"10.1080/15257770.2025.2529353","DOIUrl":"https://doi.org/10.1080/15257770.2025.2529353","url":null,"abstract":"<p><p>The major oxidative adenine lesion, 7,8-dihydro-8-oxoadenine (oxoA), can readily undergo further oxidation to generate the highly genotoxic DNA interstrand cross-links (ICLs). Herein we report that the presence of single-strand breaks (SSBs), the major lesion formed at sites of oxidative stress, in the form of a nick or single-nucleotide gap in the phosphodiester backbone of duplex DNA significantly increases the cross-linking yield of oxoA with all canonical nucleotides (up to 67.5%) upon oxidation. The cross-linking reaction occurs between the purine/pyrimidine moiety of a nucleotide on the complementary strand of the duplex and the oxoA modification on the template strand, which was confirmed by the experiment involving the use of 2',3'-dideoxycytosine. Interestingly, the minor cross-linking products in intact DNA saw a more significant increase in reactivity relative to the major oxoA-G ICL. SSBs in the form of a gap or nick between the reacting nucleotide and thymine residue base paired to oxoA produced the most significant increase in yield.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-19"},"PeriodicalIF":1.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association of CpG site of MTHFR gene promoter and type 2 diabetes in Moroccan population susceptibility. 摩洛哥人群MTHFR基因启动子CpG位点与2型糖尿病易感性的关系
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-10 DOI: 10.1080/15257770.2025.2532089
Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Nouha Messaoudi, Sofia Sehli, Najib Al Idrissi, Omar Abidi, Wajih Rhalem, Naima Khlil, Hassan Ghazal, Salsabil Hamdi
{"title":"Association of CpG site of <i>MTHFR</i> gene promoter and type 2 diabetes in Moroccan population susceptibility.","authors":"Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Nouha Messaoudi, Sofia Sehli, Najib Al Idrissi, Omar Abidi, Wajih Rhalem, Naima Khlil, Hassan Ghazal, Salsabil Hamdi","doi":"10.1080/15257770.2025.2532089","DOIUrl":"https://doi.org/10.1080/15257770.2025.2532089","url":null,"abstract":"<p><p>Type 2 diabetes (T2D) is a complex multifactorial metabolic disorder characterized by progressive disease progression, involving varying degrees of insulin resistance and pancreatic islet dysfunction. Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme regulating folate metabolism, and its polymorphisms have been associated with T2D. However, the methylation pattern of the <i>MTHFR</i> gene has not been previously studied. This study aimed to assess the association between T2D and the methylation profile of the <i>MTHFR</i> gene promotor in a Moroccan population. A total of 107 patients with T2D and 100 healthy controls were included in the study. The methylation status of CpG sites in the <i>MTHFR</i> gene promoter was conducted by methylation-specific PCR (MS-PCR). Statistical analyses were performed using SPSS software (version 20). The promoter region of the <i>MTHFR</i> gene was predominantly hyper-methylated in patients with T2D compared to healthy controls (OR: 2.924; 95% CI: 1.285-6.650; <i>p</i> = 0.008). The hypermethylated profile was not influenced by environmental or metabolic factors examined in this study. These findings suggest that hypermethylation of CpG sites in the <i>MTHFR</i> gene promoter is associated with T2D in the Moroccan population, highlighting a potential epigenetic mechanism contributing to the disease.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MiR-320a expression shows correlation with viral load in EBV-negative Burkitt lymphoma samples. 在ebv阴性伯基特淋巴瘤样本中,MiR-320a的表达与病毒载量相关。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-03 DOI: 10.1080/15257770.2025.2527141
Mevsim Saydam, Sercan Ergün, Ayhan Dağdemir, İbrahim Kartal, Oğuz Salih Dinçer, Seda Gün, Özlem Terzi
{"title":"MiR-320a expression shows correlation with viral load in EBV-negative Burkitt lymphoma samples.","authors":"Mevsim Saydam, Sercan Ergün, Ayhan Dağdemir, İbrahim Kartal, Oğuz Salih Dinçer, Seda Gün, Özlem Terzi","doi":"10.1080/15257770.2025.2527141","DOIUrl":"https://doi.org/10.1080/15257770.2025.2527141","url":null,"abstract":"<p><p>The Epstein-Barr virus (EBV) infection status varies among BL subtypes. There are unresolved questions regarding the contribution of EBV, which is strongly associated with Burkitt lymphoma, to BL pathogenesis. Differences between EBV-positive and EBV-negative BL have been previously reported. A long-debated and studied topic is the differing origins of EBV-positive and EBV-negative BL cells. Studies have suggested that miRNAs, which are post-transcriptional elements involved in many pathways, play a role in this process. In our study, in silico analyses and a literature review were used to identify miRNAs potentially involved in lymphoma and B lymphocyte development pathways. Three miRNAs (miR-182, miR-320a, miR-144) targeting the <i>BLIMP1</i> and <i>XBP1</i> genes, which act as regulators in B lymphocyte development, and associated with lymphoma were identified. Paraffin-embedded tissue samples from 28 patients diagnosed with BL were included in the study. Real-Time PCR analysis of the <i>BamHI-W</i> and <i>ApoB</i> regions classified the samples into EBV-positive and EBV-negative groups. Expression analysis of the selected three miRNAs was performed using Real-Time PCR. Expression of miR-320a was observed in all samples, and a positive correlation was found between miR-320a expression and the viral load in the EBV-negative group. This study aimed to gain insights into the role of EBV in BL pathogenesis and has demonstrated the potential role of miR-320a. In conclusion, our study demonstrates that specific miRNAs are significantly associated with EBV in Burkitt lymphoma. These findings highlight the potential of targeting these miRNAs for therapeutic interventions and suggest further research into their mechanisms of action.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-18"},"PeriodicalIF":1.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Boronic acid-modified nucleoside - synthesis and structural characterisation in the solid state. 硼酸修饰核苷的合成及固态结构表征。
IF 1.1 4区 生物学
Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-28 DOI: 10.1080/15257770.2025.2512848
Tabea Lenz, Marian Hebenbrock
{"title":"Boronic acid-modified nucleoside - synthesis and structural characterisation in the solid state.","authors":"Tabea Lenz, Marian Hebenbrock","doi":"10.1080/15257770.2025.2512848","DOIUrl":"https://doi.org/10.1080/15257770.2025.2512848","url":null,"abstract":"<p><p>The first structure of a boronic acid-modified nucleoside is elucidated by single-crystal X-ray diffraction and compared to its <i>tert</i>-butyldimethylsilyl-protected nucleoside derivative. Further, we present the synthetic access to boronic acid-modified -nucleosides providing alternative reaction conditions to a reported synthesis. Although the sugar pucker of the boronic acid-modified nucleoside differs slightly from other nucleosides, the geometry around the nucleobase shows a high degree of structural similarity to the nucleosides of the natural nucleobases. Inter- as well as intramolecular hydrogen bonds are present involving the boronic acid residue as donor. The interconnection <i>via</i> hydrogen bonds permits the formation of suprastructures in form of linear zigzag chains in the solid state. In conclusion, the structural influence by the boronic acid residue on the overall nucleoside's geometry was only minor which favours its potential use as functionalised thymidine analogue in deoxyoligonucleotides.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-22"},"PeriodicalIF":1.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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