Nucleosides, Nucleotides & Nucleic Acids最新文献

Boronic acid-modified nucleoside - synthesis and structural characterisation in the solid state. 硼酸修饰核苷的合成及固态结构表征。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-28 DOI: 10.1080/15257770.2025.2512848

Tabea Lenz, Marian Hebenbrock

引用次数: 0

LINC00365 serves as a prognostic biomarker for thyroid cancer and regulates cell proliferation, migration and invasion by targeting miR-485-5p. LINC00365作为甲状腺癌的预后生物标志物，通过靶向miR-485-5p调控细胞增殖、迁移和侵袭。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-26 DOI: 10.1080/15257770.2025.2522350

Henghua Xiao, Manlong Long, Jun Yang, Qiong Luo, Lingli Hu, Fang Chen

{"title":"LINC00365 serves as a prognostic biomarker for thyroid cancer and regulates cell proliferation, migration and invasion by targeting miR-485-5p.","authors":"Henghua Xiao, Manlong Long, Jun Yang, Qiong Luo, Lingli Hu, Fang Chen","doi":"10.1080/15257770.2025.2522350","DOIUrl":"https://doi.org/10.1080/15257770.2025.2522350","url":null,"abstract":"Thyroid cancer (TC) is the most prevalent form of malignancy within the endocrine system globally, and its incidence has been increasing significantly in recent years. The aim of this study was to explore the clinical role of LINC00365 in TC, and to analyze the regulatory mechanism between LINC00365 and miR-485-5p and its specific impact on TC. This study included 135 TC patients. The levels of LINC00365 in TC tissues and cells were detected by RT-qPCR. The impact of LINC00365 on TC prognosis was investigated using Kaplan-Meier curve and multivariate Cox regression analysis. Furthermore, the impact of LINC00365 on TC cell proliferation was examined via CCK-8 assay, while transwell assays were utilized to evaluate changes in migration and invasion capabilities. The regulatory interaction between LINC00365 and miR-485-5p was confirmed through dual-luciferase reporter assay. The expression of LINC00365 was upregulated in TC tissues and cells, and its high expression was closely related to the poor prognosis of TC patients. Kaplan-Meier curve revealed a notable correlation between elevated LINC00365 expression levels and reduced survival outcomes and multivariate Cox regression analysis revealed that elevated LINC00365 expression levels served as an independent prognostic indicator, significantly associated with adverse clinical outcomes in TC patients. In addition, LINC00365 negatively regulated the expression level of miR-485-5p. Inhibiting LINC00365 may suppress the proliferation, migration and invasion abilities of TC cells. LINC00365 may be a potential prognostic indicator for TC, and LINC00365 may promote tumor growth and metastasis of TC by inhibiting miR-485-5p expression.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral analysis of protonated and deprotonated form of ribonucleotides and their components at room temperature. 室温下核糖核苷酸的质子化和去质子化形式及其组分的光谱分析。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-26 DOI: 10.1080/15257770.2025.2521555

Mykhailo Dotsenko, Roman Nikolaiev, Zenovii Tkachuk

引用次数: 0

Aptamer-directed siRNA delivery systems for triple-negative breast cancer therapy. 适配体定向siRNA递送系统用于三阴性乳腺癌治疗。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-26 DOI: 10.1080/15257770.2025.2524468

Dilpreet Singh, Satvir Singh, Nitin Tandon

{"title":"Aptamer-directed siRNA delivery systems for triple-negative breast cancer therapy.","authors":"Dilpreet Singh, Satvir Singh, Nitin Tandon","doi":"10.1080/15257770.2025.2524468","DOIUrl":"https://doi.org/10.1080/15257770.2025.2524468","url":null,"abstract":"Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer characterized by the absence of estrogen, progesterone, and HER2 receptors, making it unresponsive to targeted hormonal and HER2-based therapies. Current treatment options, including chemotherapy and radiation, have limited efficacy and are associated with severe side effects, emphasizing the need for innovative therapeutic strategies. Aptamer-siRNA conjugates have emerged as a promising gene-silencing approach, leveraging the high specificity of nucleic acid aptamers to selectively deliver short interfering RNA (siRNA) to TNBC cells. Aptamers, single-stranded DNA or RNA molecules generated via SELEX, exhibit nanomolar-range binding affinities (Kd ∼0.5-2.5 nM) for TNBC biomarkers such as EGFR, EpCAM, nucleolin, and MUC1, enabling receptor-mediated internalization of siRNA. Preclinical studies have demonstrated that aptamer-siRNA conjugates enhance cellular uptake by 5-10-fold, improve gene silencing efficiency (80-95%), and extend siRNA stability in circulation (from <2 h to 6-9 h). In xenograft models, aptamer-siRNA therapies have shown tumor volume reductions of 60-85%, outperforming non-targeted siRNA and chemotherapy. However, challenges such as nuclease degradation, immune responses, endosomal escape, and large-scale production remain significant hurdles to clinical translation. Recent advances in chemical modifications, lipid-based carriers, and artificial intelligence-driven aptamer design are addressing these limitations, paving the way for personalized, precision RNAi-based therapeutics. This review explores the mechanisms, recent advancements, challenges, and future directions of aptamer-siRNA therapeutics, providing a comprehensive analysis of their potential to revolutionize TNBC treatment by offering targeted, effective, and less toxic gene-silencing approaches.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-19"},"PeriodicalIF":1.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct regulation mechanism of immune-related genes in left-sided and right-sided colon cancer. 免疫相关基因在左右两侧结肠癌中的不同调控机制。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-23 DOI: 10.1080/15257770.2025.2521554

Tianfei Yi, Qi Liao, Zhiqin Jiang

{"title":"Distinct regulation mechanism of immune-related genes in left-sided and right-sided colon cancer.","authors":"Tianfei Yi, Qi Liao, Zhiqin Jiang","doi":"10.1080/15257770.2025.2521554","DOIUrl":"https://doi.org/10.1080/15257770.2025.2521554","url":null,"abstract":"Colon cancer is one of the most common cancers in worldwide. Emerging evidence has demonstrated distinct patterns of immune infiltration between left colon cancer (LCC) and right colon cancer (RCC), classified according to primary tumor location. However, the underlying mechanism was still unknown. Here, we identified 111 more up-regulated genes (MURGs) including PD1 (PDCD1) and CTLA4 in RCC and 166 more down-regulated genes (MDRGs) in LCC, all participating in immune-related biological processes. Notably, CD83, CXCR4 and ISL1 emerged as reversely regulated immune-related genes (IRGs) showing opposite regulation patterns between the two cancer types. Through regulatory network analysis, we found that genetic mutations predominantly drive enhanced immune infiltration in RCC through IRG up-regulation, whereas DNA methylation-mediated IRG suppression accounts for diminished immune responses in LCC. Furthermore, prognostic models based on these IRGs with high-quality were constructed in LCC and RCC respectively. In conclusion, our results provide crucial insights into the divergent immunoregulatory mechanisms governing LCC and RCC, potentially facilitating the discovery of novel biomarkers for prognosis prediction and targeted therapy.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-28"},"PeriodicalIF":1.1,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-922 alleviates LPS-induced HK-2 cell apoptosis and inflammation through TGF-β/Smad. MiR-922通过TGF-β/Smad缓解lps诱导的HK-2细胞凋亡和炎症。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-22 DOI: 10.1080/15257770.2025.2518554

Bo Yang, Li Xu, Peng Zhou, Qihao Ma, Yong Li, Danan Sun

{"title":"MiR-922 alleviates LPS-induced HK-2 cell apoptosis and inflammation through TGF-β/Smad.","authors":"Bo Yang, Li Xu, Peng Zhou, Qihao Ma, Yong Li, Danan Sun","doi":"10.1080/15257770.2025.2518554","DOIUrl":"10.1080/15257770.2025.2518554","url":null,"abstract":"Background: MicroRNAs play an extensive role in human chronic kidney diseases (CKD), but the role of miR-922 remains unclear.Purpose: This study was designed to investigate the potential mechanism by which miR-922 acts in CKD.Methods: The human proximal renal tubular cell line (HK-2) was treated by LPS to build a CKD cell model. The expressions of miR-922, TGFβR1, and p-Smad2/3 were detected by RT-qPCR or western blotting. The dual-luciferase reporter assay verified the interaction of miR-922 with TGFβR1. Cell viability and apoptosis were assessed using the CCK-8 assay and Annexin V-FITC/PI method. An ELISA kit was used for the measure of TNF-α/IL-1β levels.Results: MiR-922 was scanty in HK-2 cells with LPS treatment. MiR-922 was an upstream regulator of TGFβR1. The up-regulation of miR-922 restrained TGFβR1 expression, thereby hindering the activation of Smad2/3. Overexpression of miR-922 and inhibition of Smad2/3 alleviated the LPS-induced apoptosis and inflammation, while TGFβR1 played a pro-apoptotic and pro-inflammatory role.Conclusion: MiR-922 rescued HK-2 cell apoptosis and inflammation damage induced by LPS through negative regulation of TGF-β/Smad pathway, which may be a vital molecular mechanism of CKD.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-11"},"PeriodicalIF":1.1,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of lung squamous cell carcinoma subtypes based on STING pathway expression and validation of prognostic features. 基于STING通路表达的肺鳞状细胞癌亚型鉴定及预后特征验证

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-14 DOI: 10.1080/15257770.2025.2505467

Wenjun Liu, Dezhong Cheng

{"title":"Identification of lung squamous cell carcinoma subtypes based on STING pathway expression and validation of prognostic features.","authors":"Wenjun Liu, Dezhong Cheng","doi":"10.1080/15257770.2025.2505467","DOIUrl":"https://doi.org/10.1080/15257770.2025.2505467","url":null,"abstract":"Lung squamous cell carcinoma (LUSC), a prevalent non-small cell lung cancer subtype, demonstrates marked heterogeneity and unpredictable prognosis. This study established a prognostic model using STING pathway-related genes to stratify LUSC patients and guide immunotherapy. Through weighted gene co-expression network analysis of TCGA-LUSC data, we identified the MEbrown module containing 13 STING-associated key genes (including CD47 and CLDN5) to develop the STING Pathway Death-Related Signature (SPDRS). LASSO regression refined the model, which effectively stratified patients into distinct high- and low-risk groups with significant survival differences. High-risk patients exhibited enhanced immune infiltration, particularly T cells CD4 memory resting and M2 macrophages, along with elevated immune checkpoint expression and stromal scores. Functional analyses revealed enrichment in immune-related pathways and tumor microenvironment regulation. Drug sensitivity predictions identified potential therapeutic agents targeting SPDRS components. A nomogram integrating SPDRS with clinical factors demonstrated strong prognostic accuracy. This work provides a novel STING pathway-based stratification system that elucidates tumor microenvironment heterogeneity and informs personalized treatment strategies. The findings highlight SPDRS as both a prognostic biomarker and therapeutic response predictor, advancing precision immunotherapy in LUSC management.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-18"},"PeriodicalIF":1.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key genes in the liver of offspring from obesity maternal mice by bioinformatics analysis. 利用生物信息学分析鉴定肥胖母鼠后代肝脏关键基因。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-13 DOI: 10.1080/15257770.2025.2517378

Qiu-Tong Chen, Ming-Wei Liu, Hong-Jie Yu, Jie Zhang, Qi-Qiang He

{"title":"Identification of key genes in the liver of offspring from obesity maternal mice by bioinformatics analysis.","authors":"Qiu-Tong Chen, Ming-Wei Liu, Hong-Jie Yu, Jie Zhang, Qi-Qiang He","doi":"10.1080/15257770.2025.2517378","DOIUrl":"https://doi.org/10.1080/15257770.2025.2517378","url":null,"abstract":"Murine maternal obesity predisposes offspring to obesity and other non-communicable diseases. Fetal programming enables researchers to trace this detrimental effect in the early life of the offspring. The aim of the current study was to explore the molecular impact of maternal obesity on the livers of murine offspring at weaning age. C57BL/6 female mice were exposed to a high-fat diet to induce obesity, after which they were mated to produce offspring. At weaning age, the metabolic health of female offspring was assessed and hepatic mRNAs were investigated via mRNA high throughput sequencing. The differentially expressed genes were identified using gene and protein expression analyses. The results revealed that murine maternal obesity altered the blood parameters, liver histology and gene expression of offspring. Cyclin-dependent kinase 1 (Cdk1) and E2f transcription factor 1 (E2f1) were identified as hub genes by bioinformatics analysis. Reverse transcription-quantitative PCR, western blotting and immunohistochemical analysis all revealed that the expression of Cdk1 and E2f1 was decreased in the livers of offspring born from obese does. In conclusion, murine maternal obesity impaired lipid metabolism in the livers of their offspring at weaning age. Furthermore, Cdk1 and E2f1 were identified as hub genes in the regulatory mechanism.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-17"},"PeriodicalIF":1.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A positive correlation between the pseudorotational phase angle P and the δ_H torsion angle (H4'-C4'-C3'-H3') in nucleosides and nucleic acids. 核苷和核酸的赝旋相角P与δH扭角（H4′-C4′-C3′-H3′）呈正相关。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-11 DOI: 10.1080/15257770.2025.2516597

Jesse Vanloon, Alexander Y Yan, Hongbin Yan

引用次数: 0

Co-regulation of miRNA and lncRNA on immunosuppression gene: unveiling the regulatory networks in cancer. miRNA和lncRNA对免疫抑制基因的共同调控：揭示肿瘤中的调控网络。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-06 DOI: 10.1080/15257770.2025.2514129

A G Dharini, Priyatharcini Kejamurthy, K T Ramya Devi

{"title":"Co-regulation of miRNA and lncRNA on immunosuppression gene: unveiling the regulatory networks in cancer.","authors":"A G Dharini, Priyatharcini Kejamurthy, K T Ramya Devi","doi":"10.1080/15257770.2025.2514129","DOIUrl":"https://doi.org/10.1080/15257770.2025.2514129","url":null,"abstract":"Cancer cells often evade immune detection and destruction by inducing immune suppression genes, which include CTLA-4, TGF-β, and PD-L1, that inhibit immune responses and promote tumour progression. Recent studies have highlighted the significance of non-coding RNAs, particularly microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), in regulating these immune suppression pathways. miRNAs, short RNA molecules that target mRNA of immune genes at the post-transcription level and influence gene expression. Similarly, lncRNAs, which act as molecular scaffolds, sponges, or regulators of gene expression, are involved in modulating immune responses by interacting with miRNAs or directly binding to immune-related genes. This review explores the complex interplay between miRNAs, lncRNAs, and immune suppression genes, detailing how these non-coding RNAs contribute to immune evasion in cancer. Furthermore, the therapeutic potential of targeting these regulatory networks is examined, highlighting current strategies and challenges in using miRNA and lncRNA modulators to enhance anti-tumour immunity. Understanding these intricate regulatory networks offers new insights into the mechanisms of immune suppression in cancer and opens avenues for developing novel therapeutic interventions to restore immune surveillance and improve the efficacy of cancer immunotherapies.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-30"},"PeriodicalIF":1.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0