Nucleosides, Nucleotides & Nucleic Acids最新文献

Visualizing NEK9 in action: aptamer-based fluorescent probes for real-time live-cell imaging. 可视化NEK9的作用：用于实时活细胞成像的适配体荧光探针。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-09-10 DOI: 10.1080/15257770.2025.2553383

Liyu Zhang, Ying Yang, Lidangzhi Mo, Fengyu Che, Benchang Li, Hong Lei, Ying Sun, Yafei Zhou, Chenlu Zhang, Yaqian Guo

{"title":"Visualizing NEK9 in action: aptamer-based fluorescent probes for real-time live-cell imaging.","authors":"Liyu Zhang, Ying Yang, Lidangzhi Mo, Fengyu Che, Benchang Li, Hong Lei, Ying Sun, Yafei Zhou, Chenlu Zhang, Yaqian Guo","doi":"10.1080/15257770.2025.2553383","DOIUrl":"https://doi.org/10.1080/15257770.2025.2553383","url":null,"abstract":"Live-cell imaging of intracellular proteins enables real-time observation of protein dynamics under near-physiological conditions, providing pivotal insights for both fundamental life science research and medical applications. However, due to limitations such as poor probe permeability and cytotoxicity associated with conventional antibody-based or genetically encoded labeling techniques, live-cell imaging remains a significant challenging. To address these limitations, here in this study, we developed and rigorously validated a novel aptamer-based fluorescent probe for real-time imaging of NEK9 kinase in living cells. First, through in vitro capture-SELEX, a DNA aptamer termed as Apt-011 which could selectively bind NEK9 was identified. Further, capitalizing on the small size, low immunogenicity, and synthetic flexibility of aptamers, we engineered a \"signal-on\" NEK9-specific aptamer-based fluorescent probe platform. This design leverages the aptamer's target--induced conformational change to physically separate the fluorophore-quencher pair, and it has been validated that this fluorescent probe platform could successfully visualize intracellular NEK9 in live-cells without obvious cytotoxicity (cell viability > 95% at working concentrations), offering new opportunities to study NEK9-associated signaling pathways. This work not only provides a robust tool for kinase research but also establishes a generalizable strategy to overcome key bottlenecks in live-cell imaging through rational aptamer engineering.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-28"},"PeriodicalIF":1.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To investigate the molecular mechanism and prognostic value of miR-551a in glioma based on bioinformatics. 基于生物信息学研究miR-551a在胶质瘤中的分子机制及预后价值。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-09-01 DOI: 10.1080/15257770.2025.2551841

Jiawei Liang, Chaoqiang Zeng, Wendi Li, Peng Zhou

{"title":"To investigate the molecular mechanism and prognostic value of miR-551a in glioma based on bioinformatics.","authors":"Jiawei Liang, Chaoqiang Zeng, Wendi Li, Peng Zhou","doi":"10.1080/15257770.2025.2551841","DOIUrl":"https://doi.org/10.1080/15257770.2025.2551841","url":null,"abstract":"Glioma represents the prevalent neoplasm type, distinguished by invasive growth and a significant rate of recurrence. This research investigates the prognostic significance and possible molecular mechanisms of miR-551a in gliomas, thereby offering a novel biomarker for gliomas treatment. This study selected 77 glioma patients and 52 craniocerebral injury patients from 2017 to 2023. The expression of miR-551a was assessed by qPCR. The ROC, Cox regression, and KM curve analyses were conducted to evaluate the diagnostic utility and prognostic value of miR-551a. The effects of inhibiting miR-551a on glioma cell functions were evaluated through CCK-8 and transwell assays. miR-551a target genes was conducted by GO and KEGG enrichment analyses, as well as PPI analysis, to elucidate potential gene regulatory relationship. miR-551a exhibited a significant up-regulation in the cerebrospinal fluid of glioma patients (P < 0.001). miR-551a possessed a robust predictive capacity for gliomas (AUC = 0.792, P < 0.001) and serves as a risk factor for unfavorable prognoses (HR = 3.858, P < 0.01), with patients exhibiting low levels of miR-551a showing favorable prognosis (P = 0.004). The inhibition of miR-551a diminished the proliferative (P < 0.05), migratory (P < 0.01), and invasive (P < 0.001) capabilities of glioma cells. miR-551a functioned as an oncogene, with CALM3 identified as critical regulatory targets (P < 0.001). miR-551a is a biological indicator for glioma prediction. It participates in the disease progression of glioma by regulating the functions of glioma cells via CALM3.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-23"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remarkable drug-like properties of mixed ligand coordination compounds having dicarboxylic acid: synthesis, characterization, molecular docking and DFT studies. 具有二羧酸的混合配体配位化合物的显著类药物性质：合成、表征、分子对接和DFT研究

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-09-01 DOI: 10.1080/15257770.2025.2552942

Nazeer Mohamed Nasar, Michael Samuel, Freeda Selva Sheela Selvaraj, Manikandan Alagumuthu, Porkodi Jeyaraman, Natarajan Raman

{"title":"Remarkable drug-like properties of mixed ligand coordination compounds having dicarboxylic acid: synthesis, characterization, molecular docking and DFT studies.","authors":"Nazeer Mohamed Nasar, Michael Samuel, Freeda Selva Sheela Selvaraj, Manikandan Alagumuthu, Porkodi Jeyaraman, Natarajan Raman","doi":"10.1080/15257770.2025.2552942","DOIUrl":"https://doi.org/10.1080/15257770.2025.2552942","url":null,"abstract":"Four mixed ligand metal complexes have been synthesized from ortho-phenylenediamine (OPD) Schiff base and a dicarboxylic acid (succinic acid). Using spectral examinations in the UV-Vis, FT-IR, mass, electron paramagnetic resonance, and nuclear magnetic resonance, the prepared ligand and the complexes have been characterized. Numerous methodologies have been employed to examine pharmacological activities, including those related to anti-oxidant, anti-microbial, and DNA binding. Utilizing the B3LYP/6-31G (d) basis set and the Gaussian-09 program, the Density Functional theory investigations have optimized the ligand and its metal complexes' molecular structures in order to investigate the theoretical properties. Furthermore, all complexes involving the interacting amino acids of the bacterial kinase (PDB ID: 7VKB) and fungal kinase (PDB ID: 6U6A) underwent molecular docking studies. In the results, metal complex [CuL(L1)] showed good DNA binding ability, antimicrobial (Lowest MIC 1.3 µg/mL), antifungal (Lowest MIC 1.2 µg/mL) and anioxidant (Lowest IC50 2.0 µg/mL) activities which ensure the good drug candidacy potentials of [CuL(L1)].","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-27"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIV-1 peptides and zinc depletion elevate the ER stress markers CHOP, ATF6, PERK, and cytokine TNF-α, as well as MHC-I, to trigger apoptosis in monocytes. HIV-1多肽和锌缺失会升高内质网应激标志物CHOP、ATF6、PERK和细胞因子TNF-α以及MHC-I，从而引发单核细胞凋亡。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-08-26 DOI: 10.1080/15257770.2025.2550965

Mahesh Malleswarapu, Naga Babu Pyadala, Kiran Alluri

{"title":"HIV-1 peptides and zinc depletion elevate the ER stress markers CHOP, ATF6, PERK, and cytokine TNF-α, as well as MHC-I, to trigger apoptosis in monocytes.","authors":"Mahesh Malleswarapu, Naga Babu Pyadala, Kiran Alluri","doi":"10.1080/15257770.2025.2550965","DOIUrl":"https://doi.org/10.1080/15257770.2025.2550965","url":null,"abstract":"Monocytes, upon activation, are known to produce substantial levels of cytokines and increased expression of MHC-I. The status of zinc and the nature of the stimulating agent significantly influence the release of various cytokines as well as the extent of MHC-I expression. However, research exploring the combined effects of inflammation and zinc deficiency remains limited. One of the primary challenges in investigating the influence of zinc status on cytokine production is the specificity of the cell types utilized in experimental settings. This study investigates zinc depletion and inflammation using THP-1 monocytes as an in vitro model, with HIV-1 viral peptide pools and TPEN (N,N,N' ,N'-Tetrakis (2-pyridylmethyl) ethylenediamine), an intracellular zinc chelator. Zinc depletion was confirmed through the application of zinquin ethyl ester via fluorescence microscopy. Additionally, we assessed the expression of zinc transporters, Bcl2 family proteins, and caspase-3 using quantitative RT-PCR and Western blot analyses. The production of TNF-α was evaluated through Golgi-Stop experiments, while MHC-I levels were measured following an 8 h incubation with HIV-1 viral peptides. The impact of PHA on monocytes concerning cytokine production and MHC-I levels was also examined. Our results revealed a rapid increase in TNF-α production and elevated MHC-I levels within 8 h post-stimulation. Furthermore, we observed an enhancement of endoplasmic reticulum (ER) stress markers, including CHOP, ATF6, and PERK, as well as BCl2 family-related genes, following treatment with the peptide pool. These findings highlight the hyperactivation of monocytes in response to HIV-1 viral peptides, which may contribute to immune system impairment through caspase-mediated apoptosis.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-15"},"PeriodicalIF":1.3,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting c-Myc with antisense oligonucleotides to induce apoptosis in tumor cells. 用反义寡核苷酸靶向c-Myc诱导肿瘤细胞凋亡。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-08-12 DOI: 10.1080/15257770.2025.2542835

Yuemei Ye, Yanhui Wang, Zhaoyun Zong, Shiyu Chen

{"title":"Targeting c-Myc with antisense oligonucleotides to induce apoptosis in tumor cells.","authors":"Yuemei Ye, Yanhui Wang, Zhaoyun Zong, Shiyu Chen","doi":"10.1080/15257770.2025.2542835","DOIUrl":"https://doi.org/10.1080/15257770.2025.2542835","url":null,"abstract":"Transcription factors (TFs) play a crucial role in tumorigenesis by driving oncogene expression in key signaling pathways. However, their small size and flat surfaces make them challenging targets for small-molecule inhibitors, while macromolecular therapies struggle to cross the cell membrane. Modulating TF activity at the genetic level offers a promising alternative. Antisense oligonucleotides (ASOs), which regulate protein expression by targeting mRNA, have emerged as effective therapeutics for previously undruggable proteins, including TFs. Over the past two decades, ASO therapeutics have advanced significantly, demonstrating long-lasting efficacy by promoting mRNA degradation. c-Myc, a key regulator of oncogene expression, drives cancer cell growth and proliferation but remains undruggable due to its nuclear localization and dynamic structure. In this study, we utilized our ASO development platform to design ASOs targeting c-Myc. Our sequence optimization algorithm achieved high accuracy, with one of three designed ASOs successfully silencing c-Myc. Ex vivo validation showed that ASO3 inhibited A549 cell growth with an IC50 of 152.5 nM. At the molecular level, ASO3 significantly reduced both c-Myc mRNA and protein expression. Functional assays, including trypan blue exclusion assay and CCK-8, confirmed that ASO3 decreased cell viability, suppressed proliferation, and induced apoptosis. These findings highlight ASO3's therapeutic potential and support further investigation as an anti-cancer agent targeting c-Myc.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-24"},"PeriodicalIF":1.3,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA PCED1B-AS1 facilitates cervical cancer progression via targeting miR-361-3p. LncRNA PCED1B-AS1通过靶向miR-361-3p促进宫颈癌进展。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-08-01 DOI: 10.1080/15257770.2025.2537150

Shuyu Wang, Junwei Wang, Ke Wang

{"title":"LncRNA PCED1B-AS1 facilitates cervical cancer progression via targeting miR-361-3p.","authors":"Shuyu Wang, Junwei Wang, Ke Wang","doi":"10.1080/15257770.2025.2537150","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537150","url":null,"abstract":"In recent years, the incidence of cervical cancer has been on the rise, making it imperative to search for targeted therapeutic tumor-associated biomarkers. PCED1B-AS1 regulates gene expression and affects cell proliferation and differentiation in tumors. However, its function and potential mechanisms in cervical cancer remain unclear. The expression of PCED1B-AS1 in cervical cancer and adjacent tissues was detected by qRT-PCR, and its clinical significance was analyzed by chi-square test and Cox model. The interaction between PCED1B-AS1 and miR-361-3p was verified by ENCORI database, and luciferase reporter assay was used to verify the interaction. The CCK-8 and Transwell assays were used to evaluate their effects on cervical cancer cell function. The expression of PCED1B-AS1 in cervical cancer tissues was significantly higher than that in adjacent tissues, and was significantly correlated with tumor grade, stage, and FIGO classification. The upregulation of PCED1B-AS1 can predict adverse prognosis in cervical cancer patients, and the expression of PCED1B-AS1 is negatively correlated with the expression of miR-361-3p. Silencing PCED1B-AS1 significantly inhibited the growth, migration, and invasion of CaSki and HeLa cells, while inhibition of miR-361-3p could diminish this effect. PCED1B-AS1 is significantly upregulated in cervical cancer tissue, and the regulatory axis formed with miR-361-3p may be involved in tumor progression. Importantly, the high expression of PCED1B-AS1 is significantly associated with poor prognosis in patients, suggesting that it not only serves as a novel molecular biomarker for the diagnosis of cervical cancer but may also provide potential intervention targets for the development of targeted therapeutic strategies.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of serum miR-892b with tumor markers and its clinical diagnostic efficacy in non-small cell lung cancer patients. 血清miR-892b与非小细胞肺癌患者肿瘤标志物的相关性及其临床诊断效果

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-08-01 DOI: 10.1080/15257770.2025.2538634

Jianliang Li, Junjie Ma, Lin Rong, Rongchen Li, Qiuyue Zhang

{"title":"Correlation of serum miR-892b with tumor markers and its clinical diagnostic efficacy in non-small cell lung cancer patients.","authors":"Jianliang Li, Junjie Ma, Lin Rong, Rongchen Li, Qiuyue Zhang","doi":"10.1080/15257770.2025.2538634","DOIUrl":"https://doi.org/10.1080/15257770.2025.2538634","url":null,"abstract":"Non-small cell lung cancer (NSCLC) poses a severe challenge to global public health safety. This research aimed at exploring the correlation between serum miR-892b and serum tumor marker (TMs) in NSCLC patients, and examining its significance in clinical diagnosis. For this research, 114 NSCLC patients and 108 healthy volunteers were enrolled. Quantitative reverse transcription PCR was employed to detect the expression of miR-892b in serum and tissues of NSCLC patients. The combined diagnostic value of miR-892b and TMs (carcinoembryonic antigen (CEA), cytokeratin 19 fragment 21-1 (CYFRA21-1), neuron-specific enolase (NSE) and carbohydrate antigen 125 (CA125)) in NSCLC was analyzed by receiver operating characteristic curve. The chi-square test was conducted to analyze the correlations between miR-892b and clinical data. Multivariate logistic regression analysis was conducted to pinpoint independent risk factors for NSCLC. MiR-892b was significantly elevated in the serum and tissues of NSCLC patients. Serum CEA, CYFRA21-1, NSE and CA125 levels were significantly higher than those of the control group. The combined diagnosis of miR-892b and TMs had higher diagnostic efficacy. High-expression miR-892b was strongly correlated with tumor-node-metastasis stage, tumor differentiation, lymph node metastasis stage, CEA, CYFRA21-1, NSE, and CA125. MiR-892b and TMs were identified as independent risk factors influencing NSCLC. MiR-892b may be a promising biomarker for NSCLC, which is important for enhancing the early diagnosis of NSCLC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of prognosis and tumor progression in cervical cancer by circ_0005728. circ_0005728对宫颈癌预后和肿瘤进展的调节作用。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-30 DOI: 10.1080/15257770.2025.2537168

Shanshan Zhang, Xiaoting Shen, Hongyin Cui

{"title":"Regulation of prognosis and tumor progression in cervical cancer by circ_0005728.","authors":"Shanshan Zhang, Xiaoting Shen, Hongyin Cui","doi":"10.1080/15257770.2025.2537168","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537168","url":null,"abstract":"To elucidate the prognostic significance and the underlying mechanism of action of circ_0005728 in patients diagnosed with cervical cancer (CCA). Intraoperative CCA and adjacent precancerous tissues from 208 patients were meticulously preserved at -80 °C. Kaplan-Meier survival curves were generated during a comprehensive 5-year postoperative follow-up. Cox analyses predicted the factors influencing prognostic progression in CCA patients. RT-qPCR detected the expression of circ_0005728. CCK8 and transwell observed cell proliferation, migration, and invasion. Flow cytometry recorded apoptotic changes. Dual luciferase reporter assay and RNA precipitation verified the interactions between circ_0005728 and miR-370-3p. High levels of circ_0005728 were observed in CCA tissues and cells. Elevated circ_0005728 expression and lymph node metastasis emerged as independent prognostic factors associated with poor outcomes in CCA patients. After silencing circ_0005728, cell functions were diminished and apoptosis increased. In addition, miR-370-3p is a downstream target gene of circ_0005728. Low expression of miR-370-3p was present in CCA tissues and was negatively correlated with circ_0005728 expression. The use of miR-370-3p inhibitor was able to induce cell proliferation, reduce apoptosis, and resist the reduction of cell migration and invasion caused by transfection of si-circ_0005728. The elevated expression of circ_0005728 in CCA patients was associated with prognostic mortality outcomes. circ_0005728 exerts its influence by targeting miR-370-3p, thereby enhancing the functional capabilities of CCA cells, diminishing apoptosis, and facilitating the malignant progression of CCA.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-20"},"PeriodicalIF":1.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144743356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-34c-3p targets FOXO3 to promote pancreatic carcinoma progression. miR-34c-3p靶向FOXO3促进胰腺癌进展。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-28 DOI: 10.1080/15257770.2025.2537167

Zhenxiong Xie, Lin Ji, Fang Wu, Hui Cao

{"title":"miR-34c-3p targets FOXO3 to promote pancreatic carcinoma progression.","authors":"Zhenxiong Xie, Lin Ji, Fang Wu, Hui Cao","doi":"10.1080/15257770.2025.2537167","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537167","url":null,"abstract":"MicroRNAs (miRNAs) have been a popular subject of tumor research including pancreatic carcinoma (PC). MicroRNA-34c-3p (miR-34c-3p) is a member of miR-34c cluster, which is strongly associated with tumorigenesis. Nonetheless, miR-34c-3p has not been explored in PC. MiR-34c-3p was taken as a target to explore its current clinical significance and related molecular mechanisms in PC. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to monitor miR-34c-3p and FOXO3 level in tissues and PC cells. Dual-Luciferase reporter assay was utilized to verifying the relationship between miR-34c-3p and FOXO3. Cell Counting Kit-8 (CCK-8) and Transwell assay were applied to detect cell proliferation, migration and invasion. MiR-34c-3p was markedly elevated in PC tissues and closely related with lymph node metastasis, post-treatment nodal margin category and degree of differentiation. MiR-34c-3p upregulation could predict poorer prognosis and higher risk of PC patients. In PC cells, overexpression of miR-34c-3p enhanced cell proliferation, migration and invasion. Moreover, miR-34c-3p negatively regulated FOXO3 to promote cellular processes. High level of miR-34c-3p is a poor prognostic factor for PC patients and miR-34c-3p promotes tumor progression by negatively regulating FOXO3.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The construction and analysis of a prognostic model based on CD8⁺ T cell immune-related genes in hepatocellular carcinoma. 基于CD8+ T细胞免疫相关基因的肝癌预后模型的构建与分析。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-18 DOI: 10.1080/15257770.2025.2531134

Zechang Zhang, Yuanyuan Yang, Yujia Zhang, Jing Wang, Huaifang Cai, Lei Yang, Wenxuan Liu

{"title":"The construction and analysis of a prognostic model based on CD8+ T cell immune-related genes in hepatocellular carcinoma.","authors":"Zechang Zhang, Yuanyuan Yang, Yujia Zhang, Jing Wang, Huaifang Cai, Lei Yang, Wenxuan Liu","doi":"10.1080/15257770.2025.2531134","DOIUrl":"https://doi.org/10.1080/15257770.2025.2531134","url":null,"abstract":"The effector functions of CD8+ T cell significantly influence the immunosuppressive microenvironment in hepatocellular carcinoma (HCC), which is intricately associated with HCC prognosis. Nevertheless, a comprehensive investigation into the relationship between CD8+ T cell immune-related genes and HCC prognosis remains lacking. This study aimed to construct a prognostic model for HCC using CD8+ T cell immune-related genes to provide insights for clinical management and prognosis. A prognostic model was constructed by incorporating 16 CD8+ T cell-specific immune-related genes, yielding area under the curve (AUC) values of 0.821, 0.796, and 0.784 for the prediction of 1-year, 3-year, and 5-year survival, respectively, via receiver operating characteristic (ROC) curve analysis. The qRT-PCR results showed that the mRNA levels of PTMA, RAC1, HSPD1, HSP90AA1, and TANK were significantly higher in HCC cells compared to normal cells (p < 0.05). Further analysis focused on the TANK gene, which was significantly upregulated in HCC tissues (p < 0.05). The CCK-8 and wound healing assays revealed a significant decrease in both the cell proliferation rate and wound-healing rate in the TANK-deficient group compared with the control group (p < 0.05). These findings may offer new insights into the clinical treatment and prognostic evaluation of HCC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-22"},"PeriodicalIF":1.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0