Nucleosides, Nucleotides & Nucleic Acids最新文献

{"title":"Targeted short read NGS of HCV 5'-UTR for genotyping and identification of Sub-variants using Ion Torrent platform.","authors":"Ritu Sagar, Pushpendra Singh, Mukesh Kumar Jogi, Sristy Shikha, Shreyansh, Arshi Anees, Diksha Saini, Meenu Jain, Soni Kumari, Suresh Hedau, Anuj Kumar, Partha Rakshit, Sandhya Kabra, Dinesh Kumar, Pramod Kumar","doi":"10.1080/15257770.2025.2566506","DOIUrl":"https://doi.org/10.1080/15257770.2025.2566506","url":null,"abstract":"<p><p>Hepatitis C Virus (HCV) is highly a mutable RNA virus, primarily due to lack of proofreading activity in RNA-dependent RNA polymerase. This intrinsic feature leads to the generation of substantial intra-host variation, resulting in the formation of viral quasispecies. The 5' untranslated region (5'UTR) of the HCV genome is highly conserved across different genotypes and serves as a widely adopted target for genotyping in clinical practice. In this study, the performance of targeted short-read next-generation sequencing of the HCV 5' UTR using Ion Torrent technology was evaluated, with the dual aim of assigning genotypes and detecting low-frequency intra-host variants. The 5'UTR-based nested RT-PCR products were amplified for five clinical plasma samples and sequenced on the Ion Gene Studio S5 platform. The resulting reads were assembled to generate consensus sequences and subjected to comprehensive analysis for iSNVs, SNPs, and indels. Sequencing of the amplicons yielded short-read sequences of 200-300 nucleotides. Phylogenetic analyses using MEGA and Interactive Tree of Life demonstrated that all isolates clustered within genotype 3, with sub-clustering indicative of subtype 3a and other intra-genotypic variants. Fourteen SNPs and several low-frequency iSNVs most notably at positions 72, 78, and 128, with variant frequencies reaching up to ˜25% were detected. These findings underscore the method's ability to discriminate both genotype and sub-variant structure. Overall, this strategy enhances molecular epidemiology, investigations, enables robust transmission tracking, and supports individualized therapeutic decision-making, particularly in resource-limited diagnostic settings.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-18"},"PeriodicalIF":1.3,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of miR-1266-5p serves as a prognostic biomarker of triple-negative breast cancer and facilitates tumor cell proliferation, migration and invasion.","authors":"Linmei Lin, Wanqi Lin, Yi Zheng","doi":"10.1080/15257770.2025.2551833","DOIUrl":"https://doi.org/10.1080/15257770.2025.2551833","url":null,"abstract":"<p><strong>Background: </strong>Triple-negative breast cancer (TNBC) is an aggressive subtype characterized by a poor prognosis. MicroRNAs (miRNAs) play a crucial regulatory role in tumorigenesis, but the specific function and mechanism of miR-1266-5p in TNBC remain unclear.</p><p><strong>Methods: </strong>This study included 118 TNBC patients, from whom both tumor and adjacent normal tissues were collected. The expression of miR-1266-5p was determined, and its association with clinicopathological features and patient prognosis was evaluated. Functional experiments were conducted using TNBC cell lines (MDA-MB-231, MDA-MB-468), assessing the impact of miR-1266-5p on cellular processes. Bioinformatics tools and dual-luciferase reporter assays were employed to identify and validate the target gene of miR-1266-5p.</p><p><strong>Results: </strong>miR-1266-5p was upregulated in TNBC tissues and cells (<i>p</i> < 0.05), and its high expression was associated with lymph node metastasis, higher histological grade, and advanced TNM stage. Kaplan-Meier analysis revealed that patients with high miR-1266-5p expression had shorter overall survival, and it was an independent prognostic factor. Functional experiments demonstrated that overexpression of miR-1266-5p significantly promoted TNBC cell proliferation, migration, and invasion (<i>p</i> < 0.05), while knockdown suppressed these phenotypes. Bioinformatics analysis and dual-luciferase assays identified MKRN1 as a direct target of miR-1266-5p.</p><p><strong>Conclusion: </strong>miR-1266-5p promotes tumor progression in TNBC by targeting MKRN1. Its high expression correlates with poor patient outcomes, suggesting that it may serve as a promising biomarker for prognosis and a potential therapeutic target in TNBC.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of miR-1303 in colon cancer and its possible molecular mechanism based on bioinformatics.","authors":"Quan Cheng, Huazhou Fu","doi":"10.1080/15257770.2025.2560352","DOIUrl":"https://doi.org/10.1080/15257770.2025.2560352","url":null,"abstract":"<p><p>Colon cancer is a leading global malignancy with significant health burden. As key regulators of tumorigenesis, microRNAs (miRNAs) are implicated in colon cancer progression, yet the role of miR-1303-its clinical significance and molecular mechanism-remains unclear. This study aimed to investigate the regulatory effects of miR-1303 and validate its potential as an independent prognostic indicator. miR-1303 expression was analyzed <i>via</i> qRT-PCR in 117 paired CRC tissues and adjacent normal tissues. Clinical relevance was evaluated <i>via</i> Kaplan-Meier survival curves and Cox proportional hazards modeling. Functional assays (CCK-8, Transwell migration/invasion, luciferase reporter) were performed in SW480 and HCT116 cells. Target genes were predicted using miRDB and TargetScan. Rescue experiments confirmed miR-1303 regulates CRC progression by targeting TMEM108. miR-1303 was significantly upregulated in colon cancer tissues compared to normal tissues (<i>p</i> < 0.001) and correlated with advanced TNM stage (<i>p</i> = 0.021), lymph node metastasis (<i>p</i> = 0.005), poor differentiation (<i>p</i> = 0.031), and larger tumor size (<i>p</i> = 0.044). High miR-1303 expression predicted poorer overall survival (<i>p</i> = 0.001) and was recognized as an independent predictor of prognosis (HR = 2.096, 95% CI = 1.080-4.071). Functional studies revealed that miR-1303 inhibition suppressed colon cancer cell proliferation, migration, and invasion. Mechanistically, miR-1303 directly targeted the TMEM108, leading to its post-transcriptional repression. Upregulated miR-1303 in colon cancer served as a poor prognosis predictor. miR-1303 promotes tumor progression in colon cancer by targeting TMEM108.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-16"},"PeriodicalIF":1.3,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visualizing NEK9 in action: aptamer-based fluorescent probes for real-time live-cell imaging.","authors":"Liyu Zhang, Ying Yang, Lidangzhi Mo, Fengyu Che, Benchang Li, Hong Lei, Ying Sun, Yafei Zhou, Chenlu Zhang, Yaqian Guo","doi":"10.1080/15257770.2025.2553383","DOIUrl":"https://doi.org/10.1080/15257770.2025.2553383","url":null,"abstract":"<p><p>Live-cell imaging of intracellular proteins enables real-time observation of protein dynamics under near-physiological conditions, providing pivotal insights for both fundamental life science research and medical applications. However, due to limitations such as poor probe permeability and cytotoxicity associated with conventional antibody-based or genetically encoded labeling techniques, live-cell imaging remains a significant challenging. To address these limitations, here in this study, we developed and rigorously validated a novel aptamer-based fluorescent probe for real-time imaging of NEK9 kinase in living cells. First, through <i>in vitro</i> capture-SELEX, a DNA aptamer termed as Apt-011 which could selectively bind NEK9 was identified. Further, capitalizing on the small size, low immunogenicity, and synthetic flexibility of aptamers, we engineered a \"signal-on\" NEK9-specific aptamer-based fluorescent probe platform. This design leverages the aptamer's target--induced conformational change to physically separate the fluorophore-quencher pair, and it has been validated that this fluorescent probe platform could successfully visualize intracellular NEK9 in live-cells without obvious cytotoxicity (cell viability > 95% at working concentrations), offering new opportunities to study NEK9-associated signaling pathways. This work not only provides a robust tool for kinase research but also establishes a generalizable strategy to overcome key bottlenecks in live-cell imaging through rational aptamer engineering.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-28"},"PeriodicalIF":1.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A narrative review of potential therapies for the treatment of myocardial tissue in relation to heart failure.","authors":"Philippe Germain, Catherine Grillon, Khue Vu Nguyen","doi":"10.1080/15257770.2025.2550969","DOIUrl":"https://doi.org/10.1080/15257770.2025.2550969","url":null,"abstract":"<p><p>Heart failure (HF) is not a disease but a combination of signs and symptoms caused by the failure of the heart to pump blood to support the circulatory system at rest or during activity. HF is the potential end stage of all heart diseases in which cardiomyopathies are a diverse group of cardiac disorders with distinct phenotypes, depending on the protein and pathways affected. Cardiomyopathies represent major causes of morbidity and mortality at all ages in humans in which hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), are the most common. Among the different common diagnostic tests for heart failure such as physical examination, blood tests, chest X-rays, electrocardiogram (ECG), etc. ultrasound has also been used not only for diagnosis such as echocardiography but also for therapeutic purposes. The development of therapeutic strategies for HF aiming to improve the heart's function, delay progression of HF, and treat HF symptoms as well as to stimulate the capacity to regenerate cardiomyocytes <i>via</i> stem cell therapy have been under intensive research interest. This narrative review aims to present the current understanding of the pathogenesis, diagnosis, and treatment of HF. Furthermore, as a perspective, this review navigates emerging therapies for HF by emphasizing on the use of low-intensity pulsed ultrasound (LIPUS) as a noninvasive therapy for (1) stimulation of the myocardial tissue reconstruction mechanisms; and (2) exploration of the molecular mechanisms behind the mechanotransduction from the muscle LIM protein (MLP), which is believed to be involved in human HF, using its expression vector <i>via</i> glycosylphosphatidylinositol, GPI, anchor.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-29"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To investigate the molecular mechanism and prognostic value of miR-551a in glioma based on bioinformatics.","authors":"Jiawei Liang, Chaoqiang Zeng, Wendi Li, Peng Zhou","doi":"10.1080/15257770.2025.2551841","DOIUrl":"https://doi.org/10.1080/15257770.2025.2551841","url":null,"abstract":"<p><p>Glioma represents the prevalent neoplasm type, distinguished by invasive growth and a significant rate of recurrence. This research investigates the prognostic significance and possible molecular mechanisms of miR-551a in gliomas, thereby offering a novel biomarker for gliomas treatment. This study selected 77 glioma patients and 52 craniocerebral injury patients from 2017 to 2023. The expression of miR-551a was assessed by qPCR. The ROC, Cox regression, and KM curve analyses were conducted to evaluate the diagnostic utility and prognostic value of miR-551a. The effects of inhibiting miR-551a on glioma cell functions were evaluated through CCK-8 and transwell assays. miR-551a target genes was conducted by GO and KEGG enrichment analyses, as well as PPI analysis, to elucidate potential gene regulatory relationship. miR-551a exhibited a significant up-regulation in the cerebrospinal fluid of glioma patients (P < 0.001). miR-551a possessed a robust predictive capacity for gliomas (AUC = 0.792, P < 0.001) and serves as a risk factor for unfavorable prognoses (HR = 3.858, P < 0.01), with patients exhibiting low levels of miR-551a showing favorable prognosis (P = 0.004). The inhibition of miR-551a diminished the proliferative (P < 0.05), migratory (P < 0.01), and invasive (P < 0.001) capabilities of glioma cells. miR-551a functioned as an oncogene, with CALM3 identified as critical regulatory targets (P < 0.001). miR-551a is a biological indicator for glioma prediction. It participates in the disease progression of glioma by regulating the functions of glioma cells via CALM3.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-23"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remarkable drug-like properties of mixed ligand coordination compounds having dicarboxylic acid: synthesis, characterization, molecular docking and DFT studies.","authors":"Nazeer Mohamed Nasar, Michael Samuel, Freeda Selva Sheela Selvaraj, Manikandan Alagumuthu, Porkodi Jeyaraman, Natarajan Raman","doi":"10.1080/15257770.2025.2552942","DOIUrl":"https://doi.org/10.1080/15257770.2025.2552942","url":null,"abstract":"<p><p>Four mixed ligand metal complexes have been synthesized from <i>ortho</i>-phenylenediamine (OPD) Schiff base and a dicarboxylic acid (succinic acid). Using spectral examinations in the UV-Vis, FT-IR, mass, electron paramagnetic resonance, and nuclear magnetic resonance, the prepared ligand and the complexes have been characterized. Numerous methodologies have been employed to examine pharmacological activities, including those related to anti-oxidant, anti-microbial, and DNA binding. Utilizing the B3LYP/6-31G (d) basis set and the Gaussian-09 program, the Density Functional theory investigations have optimized the ligand and its metal complexes' molecular structures in order to investigate the theoretical properties. Furthermore, all complexes involving the interacting amino acids of the bacterial kinase (PDB ID: 7VKB) and fungal kinase (PDB ID: 6U6A) underwent molecular docking studies. In the results, metal complex [CuL(L₁)] showed good DNA binding ability, antimicrobial (Lowest MIC 1.3 µg/mL), antifungal (Lowest MIC 1.2 µg/mL) and anioxidant (Lowest IC₅₀ 2.0 µg/mL) activities which ensure the good drug candidacy potentials of [CuL(L₁)].</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-27"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HIV-1 peptides and zinc depletion elevate the ER stress markers CHOP, ATF6, PERK, and cytokine TNF-α, as well as MHC-I, to trigger apoptosis in monocytes.","authors":"Mahesh Malleswarapu, Naga Babu Pyadala, Kiran Alluri","doi":"10.1080/15257770.2025.2550965","DOIUrl":"https://doi.org/10.1080/15257770.2025.2550965","url":null,"abstract":"<p><p>Monocytes, upon activation, are known to produce substantial levels of cytokines and increased expression of MHC-I. The status of zinc and the nature of the stimulating agent significantly influence the release of various cytokines as well as the extent of MHC-I expression. However, research exploring the combined effects of inflammation and zinc deficiency remains limited. One of the primary challenges in investigating the influence of zinc status on cytokine production is the specificity of the cell types utilized in experimental settings. This study investigates zinc depletion and inflammation using THP-1 monocytes as an <i>in vitro</i> model, with HIV-1 viral peptide pools and TPEN (<i>N</i>,<i>N</i>,<i>N</i>' ,<i>N</i>'-Tetrakis (2-pyridylmethyl) ethylenediamine), an intracellular zinc chelator. Zinc depletion was confirmed through the application of zinquin ethyl ester <i>via</i> fluorescence microscopy. Additionally, we assessed the expression of zinc transporters, Bcl2 family proteins, and caspase-3 using quantitative RT-PCR and Western blot analyses. The production of TNF-α was evaluated through Golgi-Stop experiments, while MHC-I levels were measured following an 8 h incubation with HIV-1 viral peptides. The impact of PHA on monocytes concerning cytokine production and MHC-I levels was also examined. Our results revealed a rapid increase in TNF-α production and elevated MHC-I levels within 8 h post-stimulation. Furthermore, we observed an enhancement of endoplasmic reticulum (ER) stress markers, including CHOP, ATF6, and PERK, as well as BCl2 family-related genes, following treatment with the peptide pool. These findings highlight the hyperactivation of monocytes in response to HIV-1 viral peptides, which may contribute to immune system impairment through caspase-mediated apoptosis.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-15"},"PeriodicalIF":1.3,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in synthesis of nitrogen-heterocyclic thioglycosides as potential antiviral agents.","authors":"Mamdouh A Abu-Zaied, Galal A Nawwar, Galal H Elgemeie","doi":"10.1080/15257770.2025.2529357","DOIUrl":"https://doi.org/10.1080/15257770.2025.2529357","url":null,"abstract":"<p><p>This review comprehensively examines nitrogen heterocyclic thioglycosides as promising therapeutic candidates for the development of antiviral drugs. Our aim is to explore recent advancements and the therapeutic potential of their analogues. By integrating knowledge from diverse fields, we seek to understand how these compounds can be leveraged to create effective and targeted antiviral therapies. Our findings underscore the significant potential of nitrogen heterocyclic thioglycosides as viable options for future antiviral drug development, emphasizing their ability to specifically engage and modulate key pathways. This study concludes by highlighting the crucial role of these compounds in medicinal chemistry and their promise in contributing to the creation of novel and potent antiviral agents.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-53"},"PeriodicalIF":1.3,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of differentially expressed genes and biological information between rheumatoid arthritis and osteoarthritis based on the GEO database.","authors":"Zeli Li, Li Guohai, Qianwen Xiong, Jietong Zhang, Zhicheng Li, Jiuhong He, Siying Wang, Shuwen Li","doi":"10.1080/15257770.2025.2540414","DOIUrl":"https://doi.org/10.1080/15257770.2025.2540414","url":null,"abstract":"<p><p>In the present study, we investigated the relationship between rheumatoid arthritis (RA) and knee osteoarthritis (OA) using bioinformatics, aiming to identify the differentially expressed genes (DEGs) of RA and explore the possible mechanism of RA. The GSE55584 and GSE153015 microarray datasets for RA and OA gene expression profiles were acquired from the Gene Expression Omnibus (GEO) database. The DEGs of the two datasets were obtained by R language processing and analysis. The intersecting DEGs were obtained using the Venny 2.1 platform. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genome (KEGG) enrichment analyses were performed using the DAVID platform, and the microbubble map was drawn online by importing the microbubble generation platform. All the obtained DEGs and the intersecting DEGs were imported into the STRING platform to obtain a protein-protein interaction network (PPI) and then into Cytoscape 3.9.1 software to screen core genes (hub genes). A total of 665 DEGs were obtained from the GSE55584 and GSE153015 datasets, including 324 upregulated and 341 downregulated DEGs. GO enrichment analysis showed that the biological processes in which DEGs were mainly enriched included signal transduction, immune response, inflammatory response, adaptive immune response, and G protein-coupled receptor signalling pathway. KEGG enrichment analysis of the DEGs identified the following enriched pathways: cytokine-cytokine receptor interaction; chemokine signalling pathway; viral protein interaction with cytokines and cytokine receptors; and apoptosis. Ten core genes (hub genes) were screened out, namely, CD3D, CD27, KLRB1, CCL5, GZMB, GZMA, GZMK, GNLY, CD2, and NKG7. Among them, CD3D, CD27, KLRB1, CCL5, and GZMB were most significantly correlated with the occurrence and development of RA. In the present study, bioinformatics analysis provided supporting evidence for the biological process and key genes of RA.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-15"},"PeriodicalIF":1.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}