The construction and analysis of a prognostic model based on CD8⁺ T cell immune-related genes in hepatocellular carcinoma.

IF 1.3 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-18 DOI:10.1080/15257770.2025.2531134

Zechang Zhang, Yuanyuan Yang, Yujia Zhang, Jing Wang, Huaifang Cai, Lei Yang, Wenxuan Liu

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引用次数: 0

Abstract

The effector functions of CD8⁺ T cell significantly influence the immunosuppressive microenvironment in hepatocellular carcinoma (HCC), which is intricately associated with HCC prognosis. Nevertheless, a comprehensive investigation into the relationship between CD8⁺ T cell immune-related genes and HCC prognosis remains lacking. This study aimed to construct a prognostic model for HCC using CD8⁺ T cell immune-related genes to provide insights for clinical management and prognosis. A prognostic model was constructed by incorporating 16 CD8⁺ T cell-specific immune-related genes, yielding area under the curve (AUC) values of 0.821, 0.796, and 0.784 for the prediction of 1-year, 3-year, and 5-year survival, respectively, via receiver operating characteristic (ROC) curve analysis. The qRT-PCR results showed that the mRNA levels of PTMA, RAC1, HSPD1, HSP90AA1, and TANK were significantly higher in HCC cells compared to normal cells (p < 0.05). Further analysis focused on the TANK gene, which was significantly upregulated in HCC tissues (p < 0.05). The CCK-8 and wound healing assays revealed a significant decrease in both the cell proliferation rate and wound-healing rate in the TANK-deficient group compared with the control group (p < 0.05). These findings may offer new insights into the clinical treatment and prognostic evaluation of HCC.

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基于CD8+ T细胞免疫相关基因的肝癌预后模型的构建与分析。

CD8+ T细胞的效应功能显著影响肝细胞癌（HCC）的免疫抑制微环境，这与HCC预后有着复杂的关系。然而，对CD8+ T细胞免疫相关基因与HCC预后的关系的全面研究仍然缺乏。本研究旨在利用CD8+ T细胞免疫相关基因构建HCC预后模型，为临床管理和预后提供参考。纳入16个CD8+ T细胞特异性免疫相关基因构建预后模型，通过受试者工作特征（ROC）曲线分析，预测1年、3年和5年生存的曲线下面积（AUC）分别为0.821、0.796和0.784。qRT-PCR结果显示，HCC细胞中PTMA、RAC1、HSPD1、HSP90AA1和TANK mRNA表达水平明显高于正常细胞(p p p p

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来源期刊

Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

6 months

期刊介绍： Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.