Nucleosides, Nucleotides & Nucleic Acids最新文献_第2页

A chemical labeling method for selective conjugation to DNA 3'-phosphoglycolate termini. DNA 3'-磷酸乙醇酸末端选择性偶联的化学标记方法。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-03-20 DOI: 10.1080/15257770.2026.2645371

Ryosuke Morozumi, Naoto Shimizu, Tomoko Amimoto, Junpei Yamamoto, Masataka Tsuda

{"title":"A chemical labeling method for selective conjugation to DNA 3'-phosphoglycolate termini.","authors":"Ryosuke Morozumi, Naoto Shimizu, Tomoko Amimoto, Junpei Yamamoto, Masataka Tsuda","doi":"10.1080/15257770.2026.2645371","DOIUrl":"https://doi.org/10.1080/15257770.2026.2645371","url":null,"abstract":"DNA strand breaks with chemically modified termini are a critical form of damage caused by ionizing radiation, environmental genotoxins, and therapeutic agents. Among them, 3'-phosphoglycolate (3'-PG) termini are particularly problematic, as they obstruct DNA repair and contribute to genome instability. Several indirect detection strategies have provided valuable insights into 3'-PG lesions; however, the lack of a method for direct and specific labeling of 3'-PG termini continues to limit detailed analyses of their distribution and repair in biological systems. We report a novel chemical labeling method that enables the selective conjugation of biotin to the 3'-PG terminus of synthetic oligonucleotides. The method involves carbodiimide-mediated activation of the 3'-PG carboxyl group using EDC·HCl (1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and NHS (N-hydroxysuccinimide), followed by coupling with Biotin-PEG11-Amine to form a stable amide bond. This reaction proceeds specifically at the 3'-PG terminus without modifying 3'-phosphate ends. Under the conditions used, the labeling efficiency was approximately 50%. Our results demonstrate the feasibility of chemically tagging 3'-PG ends with high specificity in vitro, providing a foundation for future studies aimed at visualizing and quantifying 3'-PG lesions in more complex biological systems.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-11"},"PeriodicalIF":1.3,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer-targeted hybrid nanoparticles based on human exosomes and LXR agonist-loaded liposomes for enhanced anti-AML therapy. 基于人外泌体和负载LXR激动剂脂质体的适体靶向杂交纳米颗粒增强抗aml治疗。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-03-19 DOI: 10.1080/15257770.2026.2644606

Liyu Zhang, Ying Yang, Lidangzhi Mo, Fengyu Che, Benchang Li, Hong Lei, Ying Sun, Yafei Zhou, Chenlu Zhang, Yaqian Guo

{"title":"Aptamer-targeted hybrid nanoparticles based on human exosomes and LXR agonist-loaded liposomes for enhanced anti-AML therapy.","authors":"Liyu Zhang, Ying Yang, Lidangzhi Mo, Fengyu Che, Benchang Li, Hong Lei, Ying Sun, Yafei Zhou, Chenlu Zhang, Yaqian Guo","doi":"10.1080/15257770.2026.2644606","DOIUrl":"https://doi.org/10.1080/15257770.2026.2644606","url":null,"abstract":"Despite advances in acute myeloid leukemia (AML) research, the treatment of AML is still inadequate, with relapse rates exceeding 50% in high-risk patients. There is an urgent need for the development of novel and synergistic therapies. In this study, we devised a Liver X Receptor (LXR) agonist, T0901317, employing the CD123 aptamer as a targeting element and exosome-liposome hybrid nanoparticles as delivery vehicles: aptamer mediated exosome-liposome hybridized nanoparticles@T0901317 (A-ELHN@T0901317). The A-ELHN@T0901317 was successfully synthesized with a uniform size of 162.0 ± 2.082 nm (PDI: 0.2368 ± 0.01845), ideal encapsulation efficiency (83.17 ± 2.881% for T0901317). In vitro, A-ELHN@T0901317 exhibited higher cellular uptake in CD123+ AML cells (molm-13) compared to non-targeted nanoparticles and a ∼50% reduction in cell proliferation relative to the control (p < 0.01). It induced G0/G1 cell cycle arrest and increased apoptosis via LXR-mediated cholesterol metabolism regulation (up-regulation of ARG1, ABCA1, ABCG1, LXRβ, FASN, ACACA, SREBF1, CDKN1A, and SCD genes). In vivo, A-ELHN@T0901317 group exhibited a substantially smaller fold increase in tumor fluorescence (21.38 ± 5.428-fold) than the control group (61.57 ± 17.73-fold, p < 0.0001), while significantly reduced damage to normal organs. Our findings suggest that A-ELHN@T0901317 represents a novel delivery method for the treatment of AML and highlights a promising direction for tumor-targeted therapy.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-39"},"PeriodicalIF":1.3,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improve the efficiency of gene silencing through 2'-O-methyl modification of the bases in siRNA terminus. 通过对siRNA末端碱基进行2'- o -甲基修饰，提高基因沉默效率。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-03-09 DOI: 10.1080/15257770.2026.2640829

Shixu Kou, Yu Zhang, Yuxuan Qian, Xuefeng Ding, Jian Jin, Zhaoqi Yang

{"title":"Improve the efficiency of gene silencing through 2'-O-methyl modification of the bases in siRNA terminus.","authors":"Shixu Kou, Yu Zhang, Yuxuan Qian, Xuefeng Ding, Jian Jin, Zhaoqi Yang","doi":"10.1080/15257770.2026.2640829","DOIUrl":"https://doi.org/10.1080/15257770.2026.2640829","url":null,"abstract":"Small interfering RNA (siRNA) holds considerable potential in the fields of biomedical research and therapy, primarily due to its high degree of specificity and its ability to elicit potent silencing effects. However, the application of this technology is faced with critical challenges, including low delivery efficiency, endosomal escape barriers, and toxicity/immunogenicity. Chemical modification is a core strategy for the optimization of siRNA performance. Among these chemical modifications, 2'-O-methyl (2'-OMe) was selected for its well-documented ability to enhance nuclease resistance and mitigate immunostimulatory effects. From a panel of prescreened MMP7-targeting siRNAs, a lead candidate was identified, and subsequent derivatives were generated by introducing distinct 2'-OMe modification patterns at both termini of the siRNA duplex. To evaluate silencing efficiency, subsequent assays were performed by quantifying MMP7 mRNA and protein expression levels in A549 cells. Notably, 2'-O-methyl (2'-OMe) modifications at both termini of the siRNA duplex significantly enhanced silencing activity, with the specific number and precise positions of modifications also contributing to this effect. This study provides experimental validation for a strategy involving limited dual-terminal 2'-OMe modifications in the development of RNAi-based therapeutics. Moreover, the demonstrated efficacy of this modification pattern offers valuable insights for the chemical optimization of other siRNA candidates.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-12"},"PeriodicalIF":1.3,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aberrant miR-410-5p expression occurs in human lumbar intervertebral disk degeneration and improves nucleus pulposus cell degeneration by targeting AIFM1. miR-410-5p的异常表达发生在人腰椎间盘退变中，并通过靶向AIFM1改善髓核细胞退变。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-03-02 DOI: 10.1080/15257770.2026.2624437

Feng Wang, Jiayue Zhang, Liqi Zhao, Yuhui Ji

{"title":"Aberrant miR-410-5p expression occurs in human lumbar intervertebral disk degeneration and improves nucleus pulposus cell degeneration by targeting AIFM1.","authors":"Feng Wang, Jiayue Zhang, Liqi Zhao, Yuhui Ji","doi":"10.1080/15257770.2026.2624437","DOIUrl":"https://doi.org/10.1080/15257770.2026.2624437","url":null,"abstract":"Lumbar intervertebral disk degeneration (IDD) is a degenerative disease characterized by nucleus pulposus cells (NPCs) senescence and dysfunction, and microRNAs (miRNAs) play crucial regulatory roles in its pathogenesis. However, the role of miR-410-5p in IDD remains unclear. To investigate the expression pattern and diagnostic value of miR-410-5p in IDD, and explore its molecular mechanism in regulating NPCs degeneration by targeting AIFM1. Bioinformatics analysis was performed using the GEO dataset GSE116726 to screen differentially expressed miRNAs. Clinical samples from 95 IDD patients and 81 healthy controls were collected to detect serum miR-410-5p and AIFM1 expression by RT-qPCR, and their diagnostic value was evaluated by ROC curve and logistic regression. A TNF-α-induced NPCs senescence model was constructed, and the regulatory relationship between miR-410-5p and AIFM1 was verified by dual-luciferase reporter assay. The effects of miR-410-5p and AIFM1 on NPCs senescence, inflammation, and ECM metabolism were detected by SA-β-gal staining, ELISA, and RT-qPCR. MiR-410-5p was significantly downregulated in IDD patients and TNF-α-induced NPCs, and it has good diagnostic value for IDD. MiR-410-5p directly targeted AIFM1, and their expressions were negatively correlated. MiR-410-5p overexpression reduced senescence markers (p16, p21, SA-β-gal activity), inflammatory factors (IL-1β and IL-6), and promoted ECM-related genes (COL2A1, ACAN), while AIFM1 overexpression reversed these effects. MiR-410-5p is downregulated in IDD and serves as a potential diagnostic marker. It alleviates TNF-α-induced NPCs senescence, inflammation, and ECM degradation by targeting AIFM1, providing a new target for IDD therapy.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.3,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear receptors: a pandora of hope for metabolic syndrome. 核受体：代谢综合征的希望之星。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-02-14 DOI: 10.1080/15257770.2026.2620694

Swati Singh, Rohini Agrawal

{"title":"Nuclear receptors: a pandora of hope for metabolic syndrome.","authors":"Swati Singh, Rohini Agrawal","doi":"10.1080/15257770.2026.2620694","DOIUrl":"https://doi.org/10.1080/15257770.2026.2620694","url":null,"abstract":"Metabolic Syndrome can be defined as a cluster of abnormalities which includes obesity, dyslipidemia, hypertension, and insulin resistance. The intricate connections between genetic and environmental variables are involved in the development of metabolic syndrome (MetS). Nuclear receptors (NR), a family of transcription factors, are the key participants in onset and progression of MetS because their major mechanism of action is to modulate various metabolic pathways. The main characteristic features of MetS include abnormal distribution of adipose tissue, aberrant lipid profiles, decreased glucose tolerance, and inflammation, and these all develop due to dysregulation of NR signaling. This review is an attempt to understand the principles of molecular mechanisms and functions of nuclear receptors that can be utilized in the treatment of MetS, because numerous critical processes, such as adipocyte development, lipid metabolism, glucose homeostasis, and inflammation, are strongly mediated by nuclear receptors, e.g. FXR, LXR, GR, and PPARs. In addition, understanding how nuclear receptors interact with other signaling pathways may lead to findings of new therapeutic targets for MetS. Understanding specific methods by which nuclear receptors affect metabolic pathways would further our knowledge of the pathophysiology of MetS, and open the door to future development of novel treatment approaches.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-35"},"PeriodicalIF":1.3,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146195199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNAseq-based meta-analyses revealed tumor suppressor-inducer fusion events in liver, oral, and ovarian cancer in the Indian population: a cancer cell surviving mechanism. 基于rnaseq的荟萃分析揭示了印度人群中肝癌、口腔癌和卵巢癌的肿瘤抑制因子-诱导剂融合事件：一种癌细胞存活机制。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-02-09 DOI: 10.1080/15257770.2026.2624447

Rahul Yadav, Hafsa Khan, Poonam Singh, Pramod Kumar, Dinesh Kumar Singhal

{"title":"RNAseq-based meta-analyses revealed tumor suppressor-inducer fusion events in liver, oral, and ovarian cancer in the Indian population: a cancer cell surviving mechanism.","authors":"Rahul Yadav, Hafsa Khan, Poonam Singh, Pramod Kumar, Dinesh Kumar Singhal","doi":"10.1080/15257770.2026.2624447","DOIUrl":"https://doi.org/10.1080/15257770.2026.2624447","url":null,"abstract":"Cancer cell characteristics are determined by gene expression, influenced by genomic, epigenetic, and transcriptional modifications. Genomic rearrangements and transcriptional splicing can result in the formation of fusion genes. BCR-ABL1 is an established fusion gene employed as a biomarker in leukemia. A single gene can amalgamate with several other genes and may impact cellular fate. Ethnicity-specific variants of fusion genes have been identified, such as the TMPRSS2-ERG variation observed in prostate malignancies among African-American, Caucasian, and Japanese populations in research studies. Next-generation sequencing has provided a new method for predicting genomic and transcriptomic changes. We aim to identify fusion genes in the Indian population using cancer samples to enhance diagnostic outcomes. This study performed a meta-analysis of tumor-specific RNA sequencing data for liver, tongue, and ovarian cancers, which are available online. It identified known fusion genes, including TRO-MAGED2, KRT14-S100A9, RNASE10-CD38, ACTN4-ACTN1, RGPD1-RANBP2, CTSC-RAB38, C15orf57-CBX3, AMBRA1-CKAP5, ATP2B3-ATP2B4, CNKSR3-IPCEF1, E2F4-RPL14, and MZT2A-MZT2B, along with 101 novel fusion genes. Novel fusion genes GABRP_SCGB3A2 and WWOX_FUT1 were identified in all three tumor tissues. GABRP acts as a tumor inducer, whereas SCGB3A2 functions as a tumor suppressor. WWOX2 serves as a tumor suppressor, whereas FUT1 functions as a promoter of malignancy. The interplay between tumor inducers and suppressors may serve as a survival mechanism for cancer cells, a subject that has received limited research attention.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-16"},"PeriodicalIF":1.3,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, synthesis, and biological properties of novel metabolizable pH-responsive lipids for use in lipid nanoparticles for siRNA delivery and mRNA vaccination. 用于siRNA递送和mRNA疫苗接种的脂质纳米颗粒的新型代谢ph响应脂质的设计、合成和生物学特性

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-16 DOI: 10.1080/15257770.2026.2615355

Takako Niwa, Yoshiyuki Onishi, Masakazu Tamura, Yuji Kasuya, Takayoshi Nishiya, Daisuke Sugiyama, Taishi Yoshida, Miyuki Tozuka, Eiko Namba, Kazuki Miyaji, Nao Jonai, Makoto Koizumi

{"title":"Design, synthesis, and biological properties of novel metabolizable pH-responsive lipids for use in lipid nanoparticles for siRNA delivery and mRNA vaccination.","authors":"Takako Niwa, Yoshiyuki Onishi, Masakazu Tamura, Yuji Kasuya, Takayoshi Nishiya, Daisuke Sugiyama, Taishi Yoshida, Miyuki Tozuka, Eiko Namba, Kazuki Miyaji, Nao Jonai, Makoto Koizumi","doi":"10.1080/15257770.2026.2615355","DOIUrl":"https://doi.org/10.1080/15257770.2026.2615355","url":null,"abstract":"We developed pH-responsive lipids, LIPIDs-1 and LIPIDs-2, which feature an N,N-dimethylaminopropyl head and 2 hydrophobic tails connected by carbonate esters. These lipids are metabolizable owing to acetyl ester structures that can be cleaved by esterases, reducing their affinity for biological membranes after hydrolysis of the esters. We evaluated both lipids in vivo using siRNA-loaded lipid nanoparticles (LNPs) targeting Factor VII, which resulted in significant knockdown activity for up to 6 days. Toxicological studies of LNPs using LIPIDs-1 and LIPIDs-2 in rats indicated minimal liver toxicity and rapid metabolism of the lipids, confirming their safety. Moreover, we tested LNPs using LIPIDs-1 as an mRNA vaccine carrier, which resulted in strong immune responses against both ovalbumin and SARS-CoV-2 antigens with a notable Th1-biased profile. These findings suggest that LNPs made using LIPIDs-1 and LIPIDs-2 are promising candidates for drug delivery and vaccine applications.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-20"},"PeriodicalIF":1.3,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tribute to Paul F. Torrence, a brilliant chemist switching from polynucleotides to nucleosides, oligonucleotides, and back to nucleosides. 向保罗·f·托伦斯致敬，他是一位杰出的化学家，从多核苷酸到核苷、寡核苷酸，再到核苷。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-08 DOI: 10.1080/15257770.2025.2609766

Erik De Clercq

引用次数: 0

LINC00885 suppresses gastric cancer progression by sponging miR-320c and serves as a diagnostic biomarker. LINC00885通过海绵miR-320c抑制胃癌进展，可作为诊断性生物标志物。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-03 DOI: 10.1080/15257770.2025.2607721

Suyan Ding, Buluan Zhu, Tiantian Sun, Lili Qu

{"title":"LINC00885 suppresses gastric cancer progression by sponging miR-320c and serves as a diagnostic biomarker.","authors":"Suyan Ding, Buluan Zhu, Tiantian Sun, Lili Qu","doi":"10.1080/15257770.2025.2607721","DOIUrl":"https://doi.org/10.1080/15257770.2025.2607721","url":null,"abstract":"Background: Long non-coding RNAs (lncRNAs) perform critical functions in tumorigenesis and the development of malignancy. Dysregulation of LINC00885 has been associated with intricate biological processes in diverse cancer types. However, the functional significance of LINC00885 in gastric cancer (GC) and the mechanistic underpinnings governing its activity have yet to be thoroughly investigated.Methods: LINC00885 expression levels were assessed in serum specimens obtained from both healthy donors and GC patients, as well as across various GC cell lines, employing quantitative reverse transcription polymerase chain reaction (RT-qPCR). The diagnostic efficacy of LINC00885 was determined through receiver operating characteristic (ROC) curve analysis. Cellular functions (proliferation, migration, invasion) and the nature of miRNA interactions were elucidated via functional assays and rescue experiments.Results: LINC00885 expression was markedly reduced in GC and demonstrated the capacity to differentiate GC patients from healthy controls. Based on the median serum LINC00885 expression level in 218 gastric cancer patients, cases were categorized into high- and low-expression groups. Low serum LINC00885 levels were significantly associated with larger tumor size (p = 0.044) and advanced TNM stage (p = 0.022), but not with other clinicopathological parameters. Ectopic expression of LINC00885 inhibited the proliferative, migratory, and invasive capacities of GC cells. LINC00885 was demonstrated to bind directly to miR-320c and modulate GC cellular behavior. Mechanistically, the tumor-suppressive impact of LINC00885 during GC progression is likely mediated by its function as a competitive endogenous RNA (ceRNA), sequestering miR-320c.Conclusion: LINC00885 exhibits potential diagnostic value and represents a promising therapeutic target for GC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2026-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145892919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of miR-182 changes the sensitivity of triple-negative breast cancer cells to cisplatin. miR-182的敲低改变三阴性乳腺癌细胞对顺铂的敏感性。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-01-11 DOI: 10.1080/15257770.2025.2451818

Hülya Dönmez, Bahadır Batar, Burhan Turgut

{"title":"Knockdown of miR-182 changes the sensitivity of triple-negative breast cancer cells to cisplatin.","authors":"Hülya Dönmez, Bahadır Batar, Burhan Turgut","doi":"10.1080/15257770.2025.2451818","DOIUrl":"10.1080/15257770.2025.2451818","url":null,"abstract":"Breast cancer is the most common malignancy that affects women. MicroRNAs (miRNAs) play an essential role in cancer therapy and regulate many biological processes such as cisplatin resistance. The study's objective was to determine whether miR-182 dysregulation was the cause of cisplatin resistance in TNBC cell line MDA-MB-231. To determine the expression of miR-182, PCR was performed with primers specific to miR-182, and agarose gel electrophoresis was performed. To reduce the expression of miR-182 in MDA-MB-231 cells, anti-miR-182 oligonucleotides were used. RT-qPCR was used to confirm knockdown. The knockdown and control groups were treated with cisplatin at the same time. Propidium iodide (PI) and Annexin V staining were performed for apoptosis assay. Flow cytometric analysis was used to investigate the effect of miR-182 knockdown on cell cycle arrest. In comparison to untreated control MDA-MB-231 cells with MDA-MB-231 cells treated with anti-miR-182, there was a significant increase in the cisplatin-induced early apoptosis phase (p = 0.023). Also, inhibition of miR-182 significantly increased the cell cycle arrest at the G2/M phase in MDA-MB-231 cells (p = 0.031). Our results revealed that miR-182 inhibition may play a role in the overcoming of cisplatin resistance by inducing apoptosis and, cell cycle arrest in TNBC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"35-49"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0