Nucleosides, Nucleotides & Nucleic Acids最新文献

{"title":"Analysis of differentially expressed genes and biological information between rheumatoid arthritis and osteoarthritis based on the GEO database.","authors":"Zeli Li, Li Guohai, Qianwen Xiong, Jietong Zhang, Zhicheng Li, Jiuhong He, Siying Wang, Shuwen Li","doi":"10.1080/15257770.2025.2540414","DOIUrl":"https://doi.org/10.1080/15257770.2025.2540414","url":null,"abstract":"<p><p>In the present study, we investigated the relationship between rheumatoid arthritis (RA) and knee osteoarthritis (OA) using bioinformatics, aiming to identify the differentially expressed genes (DEGs) of RA and explore the possible mechanism of RA. The GSE55584 and GSE153015 microarray datasets for RA and OA gene expression profiles were acquired from the Gene Expression Omnibus (GEO) database. The DEGs of the two datasets were obtained by R language processing and analysis. The intersecting DEGs were obtained using the Venny 2.1 platform. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genome (KEGG) enrichment analyses were performed using the DAVID platform, and the microbubble map was drawn online by importing the microbubble generation platform. All the obtained DEGs and the intersecting DEGs were imported into the STRING platform to obtain a protein-protein interaction network (PPI) and then into Cytoscape 3.9.1 software to screen core genes (hub genes). A total of 665 DEGs were obtained from the GSE55584 and GSE153015 datasets, including 324 upregulated and 341 downregulated DEGs. GO enrichment analysis showed that the biological processes in which DEGs were mainly enriched included signal transduction, immune response, inflammatory response, adaptive immune response, and G protein-coupled receptor signalling pathway. KEGG enrichment analysis of the DEGs identified the following enriched pathways: cytokine-cytokine receptor interaction; chemokine signalling pathway; viral protein interaction with cytokines and cytokine receptors; and apoptosis. Ten core genes (hub genes) were screened out, namely, CD3D, CD27, KLRB1, CCL5, GZMB, GZMA, GZMK, GNLY, CD2, and NKG7. Among them, CD3D, CD27, KLRB1, CCL5, and GZMB were most significantly correlated with the occurrence and development of RA. In the present study, bioinformatics analysis provided supporting evidence for the biological process and key genes of RA.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-15"},"PeriodicalIF":1.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting c-Myc with antisense oligonucleotides to induce apoptosis in tumor cells.","authors":"Yuemei Ye, Yanhui Wang, Zhaoyun Zong, Shiyu Chen","doi":"10.1080/15257770.2025.2542835","DOIUrl":"https://doi.org/10.1080/15257770.2025.2542835","url":null,"abstract":"<p><p>Transcription factors (TFs) play a crucial role in tumorigenesis by driving oncogene expression in key signaling pathways. However, their small size and flat surfaces make them challenging targets for small-molecule inhibitors, while macromolecular therapies struggle to cross the cell membrane. Modulating TF activity at the genetic level offers a promising alternative. Antisense oligonucleotides (ASOs), which regulate protein expression by targeting mRNA, have emerged as effective therapeutics for previously undruggable proteins, including TFs. Over the past two decades, ASO therapeutics have advanced significantly, demonstrating long-lasting efficacy by promoting mRNA degradation. c-Myc, a key regulator of oncogene expression, drives cancer cell growth and proliferation but remains undruggable due to its nuclear localization and dynamic structure. In this study, we utilized our ASO development platform to design ASOs targeting c-Myc. Our sequence optimization algorithm achieved high accuracy, with one of three designed ASOs successfully silencing c-Myc. <i>Ex vivo</i> validation showed that ASO3 inhibited A549 cell growth with an IC₅₀ of 152.5 nM. At the molecular level, ASO3 significantly reduced both c-Myc mRNA and protein expression. Functional assays, including trypan blue exclusion assay and CCK-8, confirmed that ASO3 decreased cell viability, suppressed proliferation, and induced apoptosis. These findings highlight ASO3's therapeutic potential and support further investigation as an anti-cancer agent targeting c-Myc.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-24"},"PeriodicalIF":1.3,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of circular RNAs (circRNAs) in oral cancer pathogenesis and their potential as therapeutic targets.","authors":"Chandini Rajkumar, Saranya Ramsridhar, Indu Bharkavi S K, Sankara Krishnan S, Daflin Femi F, Suganya V","doi":"10.1080/15257770.2025.2543581","DOIUrl":"https://doi.org/10.1080/15257770.2025.2543581","url":null,"abstract":"<p><p>Several studies have shown the role of circRNAs in tumor cell proliferation, migration, invasion, apoptosis, angiogenesis, and recurrence, suggesting potential as a biological marker and target for therapy for preventing and therapeutic management of cancer. This systematic review summarizes the role of circRNA on OC signaling networks and reveals potential oral cancer (OC) treatment targets. The systematic review followed PRISMA guidelines for study selection, synthesis, and dissemination. PubMed, Scopus, and Web of Sciences were searched for evidence-based studies on circRNA on OC from January 2015 to December 2024. Two experts performed an unbiased inquiry and specified screening. The Cochrane Collaboration tool assessed the risk of bias (RoB) using ROBINS-I, which classifies bias as low, moderate, serious, critical, or no information. The systematic review comprised 19 papers. High-throughput sequencing techniques or circRNA microarray studies for OSCC revealed numerous differentially expressed circRNAs across OSCC tissues and neighboring tissues. Subsequently, a series of studies demonstrated that certain circRNAs serve pivotal roles in the genesis and progression of OC. Many circRNAs are essential regulators of pivotal pathways in oral malignancies. CircRNAs work by competitively binding or \"sponging\" miRs through the development of persistent complementary contacts, RNA-binding protein sponges, protein/peptide translators, and regulators of gene splicing and transcription.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA PCED1B-AS1 facilitates cervical cancer progression via targeting miR-361-3p.","authors":"Shuyu Wang, Junwei Wang, Ke Wang","doi":"10.1080/15257770.2025.2537150","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537150","url":null,"abstract":"<p><p>In recent years, the incidence of cervical cancer has been on the rise, making it imperative to search for targeted therapeutic tumor-associated biomarkers. PCED1B-AS1 regulates gene expression and affects cell proliferation and differentiation in tumors. However, its function and potential mechanisms in cervical cancer remain unclear. The expression of PCED1B-AS1 in cervical cancer and adjacent tissues was detected by qRT-PCR, and its clinical significance was analyzed by chi-square test and Cox model. The interaction between PCED1B-AS1 and miR-361-3p was verified by ENCORI database, and luciferase reporter assay was used to verify the interaction. The CCK-8 and Transwell assays were used to evaluate their effects on cervical cancer cell function. The expression of PCED1B-AS1 in cervical cancer tissues was significantly higher than that in adjacent tissues, and was significantly correlated with tumor grade, stage, and FIGO classification. The upregulation of PCED1B-AS1 can predict adverse prognosis in cervical cancer patients, and the expression of PCED1B-AS1 is negatively correlated with the expression of miR-361-3p. Silencing PCED1B-AS1 significantly inhibited the growth, migration, and invasion of CaSki and HeLa cells, while inhibition of miR-361-3p could diminish this effect. PCED1B-AS1 is significantly upregulated in cervical cancer tissue, and the regulatory axis formed with miR-361-3p may be involved in tumor progression. Importantly, the high expression of PCED1B-AS1 is significantly associated with poor prognosis in patients, suggesting that it not only serves as a novel molecular biomarker for the diagnosis of cervical cancer but may also provide potential intervention targets for the development of targeted therapeutic strategies.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of serum miR-892b with tumor markers and its clinical diagnostic efficacy in non-small cell lung cancer patients.","authors":"Jianliang Li, Junjie Ma, Lin Rong, Rongchen Li, Qiuyue Zhang","doi":"10.1080/15257770.2025.2538634","DOIUrl":"https://doi.org/10.1080/15257770.2025.2538634","url":null,"abstract":"<p><p>Non-small cell lung cancer (NSCLC) poses a severe challenge to global public health safety. This research aimed at exploring the correlation between serum miR-892b and serum tumor marker (TMs) in NSCLC patients, and examining its significance in clinical diagnosis. For this research, 114 NSCLC patients and 108 healthy volunteers were enrolled. Quantitative reverse transcription PCR was employed to detect the expression of miR-892b in serum and tissues of NSCLC patients. The combined diagnostic value of miR-892b and TMs (carcinoembryonic antigen (CEA), cytokeratin 19 fragment 21-1 (CYFRA21-1), neuron-specific enolase (NSE) and carbohydrate antigen 125 (CA125)) in NSCLC was analyzed by receiver operating characteristic curve. The chi-square test was conducted to analyze the correlations between miR-892b and clinical data. Multivariate logistic regression analysis was conducted to pinpoint independent risk factors for NSCLC. MiR-892b was significantly elevated in the serum and tissues of NSCLC patients. Serum CEA, CYFRA21-1, NSE and CA125 levels were significantly higher than those of the control group. The combined diagnosis of miR-892b and TMs had higher diagnostic efficacy. High-expression miR-892b was strongly correlated with tumor-node-metastasis stage, tumor differentiation, lymph node metastasis stage, CEA, CYFRA21-1, NSE, and CA125. MiR-892b and TMs were identified as independent risk factors influencing NSCLC. MiR-892b may be a promising biomarker for NSCLC, which is important for enhancing the early diagnosis of NSCLC.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of prognosis and tumor progression in cervical cancer by circ_0005728.","authors":"Shanshan Zhang, Xiaoting Shen, Hongyin Cui","doi":"10.1080/15257770.2025.2537168","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537168","url":null,"abstract":"<p><p>To elucidate the prognostic significance and the underlying mechanism of action of circ_0005728 in patients diagnosed with cervical cancer (CCA). Intraoperative CCA and adjacent precancerous tissues from 208 patients were meticulously preserved at -80 °C. Kaplan-Meier survival curves were generated during a comprehensive 5-year postoperative follow-up. Cox analyses predicted the factors influencing prognostic progression in CCA patients. RT-qPCR detected the expression of circ_0005728. CCK8 and transwell observed cell proliferation, migration, and invasion. Flow cytometry recorded apoptotic changes. Dual luciferase reporter assay and RNA precipitation verified the interactions between circ_0005728 and miR-370-3p. High levels of circ_0005728 were observed in CCA tissues and cells. Elevated circ_0005728 expression and lymph node metastasis emerged as independent prognostic factors associated with poor outcomes in CCA patients. After silencing circ_0005728, cell functions were diminished and apoptosis increased. In addition, miR-370-3p is a downstream target gene of circ_0005728. Low expression of miR-370-3p was present in CCA tissues and was negatively correlated with circ_0005728 expression. The use of miR-370-3p inhibitor was able to induce cell proliferation, reduce apoptosis, and resist the reduction of cell migration and invasion caused by transfection of si-circ_0005728. The elevated expression of circ_0005728 in CCA patients was associated with prognostic mortality outcomes. circ_0005728 exerts its influence by targeting miR-370-3p, thereby enhancing the functional capabilities of CCA cells, diminishing apoptosis, and facilitating the malignant progression of CCA.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-20"},"PeriodicalIF":1.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144743356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-34c-3p targets FOXO3 to promote pancreatic carcinoma progression.","authors":"Zhenxiong Xie, Lin Ji, Fang Wu, Hui Cao","doi":"10.1080/15257770.2025.2537167","DOIUrl":"https://doi.org/10.1080/15257770.2025.2537167","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) have been a popular subject of tumor research including pancreatic carcinoma (PC). MicroRNA-34c-3p (miR-34c-3p) is a member of miR-34c cluster, which is strongly associated with tumorigenesis. Nonetheless, miR-34c-3p has not been explored in PC. MiR-34c-3p was taken as a target to explore its current clinical significance and related molecular mechanisms in PC. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to monitor miR-34c-3p and FOXO3 level in tissues and PC cells. Dual-Luciferase reporter assay was utilized to verifying the relationship between miR-34c-3p and FOXO3. Cell Counting Kit-8 (CCK-8) and Transwell assay were applied to detect cell proliferation, migration and invasion. MiR-34c-3p was markedly elevated in PC tissues and closely related with lymph node metastasis, post-treatment nodal margin category and degree of differentiation. MiR-34c-3p upregulation could predict poorer prognosis and higher risk of PC patients. In PC cells, overexpression of miR-34c-3p enhanced cell proliferation, migration and invasion. Moreover, miR-34c-3p negatively regulated FOXO3 to promote cellular processes. High level of miR-34c-3p is a poor prognostic factor for PC patients and miR-34c-3p promotes tumor progression by negatively regulating FOXO3.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the structural dynamics of Argonaute (AGO)-mediated gene silencing of miR-21-5p, miR-221-3p, miR-126-3p, miR-34a-5p and their role as potential biomarkers in lung cancer.","authors":"Anmol Bhatia, Atul Kumar Upadhyay, Siddharth Sharma","doi":"10.1080/15257770.2025.2535749","DOIUrl":"https://doi.org/10.1080/15257770.2025.2535749","url":null,"abstract":"<p><p>Lung Cancer is the leading cause of cancer-related deaths worldwide, with over 85% of the cases being of non-small cell lung cancer. Despite the recent advances, lung cancer remains undiagnosed until after the disease has advanced. The role of miRNAs in gene silencing is driven by RNA-induced silencing complex (RISC) consisting of Argonaute (AGO) protein. Understanding the miRNA-assisted gene regulation in lung cancer poses a prospect for an improved diagnostic and preventive measure towards the disease. This study explores miRNA interactions and their target genes in lung cancer, identifying four key miRNAs: miR-21-5p, miR-221-3p, miR-126-3p, and miR-34a-5p. These were shortlisted through their minimum free energy score, pattern conservation, functional, and network analysis. The AGO protein was retrieved, prepared for docking analysis with miRNA-mRNA duplexes, and docked using the HDOCK webserver. The docking results pointed towards the strong binding affinity of the miRNAs towards their targets and the AGO protein playing a crucial role as a driving force for gene expression. Furthermore, the miRNAs were established for their clinical relevance, particularly noting the association of high miR-21-5p expression with poor overall survival, suggesting potential avenues for further molecular investigation in lung cancer development.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-34"},"PeriodicalIF":1.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-766-3p promotes non-small cell lung cancer development through suppression of NR3C2.","authors":"Xuexi Zhang, Lei Cao, Gang Zhang","doi":"10.1080/15257770.2025.2533391","DOIUrl":"https://doi.org/10.1080/15257770.2025.2533391","url":null,"abstract":"<p><p>The incidence of non-small cell lung cancer (NSCLC) has exhibited an elevated trend yearly, seriously threatening human health. However, its molecular mechanism is still unknown. The objective of this experiment was to investigate the expression and prognostic value of miR-766-3p in the tissues of NSCLC patients, as well as to provide possible targets for the healing of NSCLC. In this study, miR-766-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2) were detected in tissues of NSCLC patients using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Survival analysis was estimated with Kaplan-Meier and Cox proportional hazards model. <i>In vitro</i> experiments included Cell Counting Kit-8 (CCK-8) viability assay, flow cytometry for apoptosis, Transwell assay for migration and invasion, and luciferase reporter assay for target genes. miR-766-3p levels were clearly elevated in tumor tissues, and high miR-766-3p levels were considered to be a poor prognostic factor. NR3C2 was clearly down-regulated in the serum of NSCLC patients. miR-766-3p overexpression stimulated the proliferation and enhanced metastatic spread of lung cancer cells, whereas down-regulation of NR3C2 reversed the effect of miR-766-3p on cellular activity. miR-766-3p promotes NSCLC development by inhibiting NR3C2 levels and is a potential biomarker. Furthermore, high levels of miR-766-3p are likely to predict poor prognosis in NSCLC.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-11"},"PeriodicalIF":1.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The construction and analysis of a prognostic model based on CD8⁺ T cell immune-related genes in hepatocellular carcinoma.","authors":"Zechang Zhang, Yuanyuan Yang, Yujia Zhang, Jing Wang, Huaifang Cai, Lei Yang, Wenxuan Liu","doi":"10.1080/15257770.2025.2531134","DOIUrl":"https://doi.org/10.1080/15257770.2025.2531134","url":null,"abstract":"<p><p>The effector functions of CD8⁺ T cell significantly influence the immunosuppressive microenvironment in hepatocellular carcinoma (HCC), which is intricately associated with HCC prognosis. Nevertheless, a comprehensive investigation into the relationship between CD8⁺ T cell immune-related genes and HCC prognosis remains lacking. This study aimed to construct a prognostic model for HCC using CD8⁺ T cell immune-related genes to provide insights for clinical management and prognosis. A prognostic model was constructed by incorporating 16 CD8⁺ T cell-specific immune-related genes, yielding area under the curve (AUC) values of 0.821, 0.796, and 0.784 for the prediction of 1-year, 3-year, and 5-year survival, respectively, <i>via</i> receiver operating characteristic (ROC) curve analysis. The qRT-PCR results showed that the mRNA levels of PTMA, RAC1, HSPD1, HSP90AA1, and TANK were significantly higher in HCC cells compared to normal cells (<i>p</i> < 0.05). Further analysis focused on the TANK gene, which was significantly upregulated in HCC tissues (<i>p</i> < 0.05). The CCK-8 and wound healing assays revealed a significant decrease in both the cell proliferation rate and wound-healing rate in the TANK-deficient group compared with the control group (<i>p</i> < 0.05). These findings may offer new insights into the clinical treatment and prognostic evaluation of HCC.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-22"},"PeriodicalIF":1.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}