Nucleosides, Nucleotides & Nucleic Acids最新文献

{"title":"LncRNA EGOT inhibits the proliferation of lung adenocarcinoma cells and is associated with poor prognosis.","authors":"Yi Ming Sheng, Ke Qiang Wang, Hu Nan Huang, Hua Qin Qiu, Jia Wu, Ping Liu","doi":"10.1080/15257770.2026.2675262","DOIUrl":"https://doi.org/10.1080/15257770.2026.2675262","url":null,"abstract":"<p><p>Long non-coding RNA (lncRNA) EGOT is dysregulated in multiple malignancies and participates in tumor progression. Its role and mechanism in lung adenocarcinoma (LUAD) were unclear. The present study set out to investigate the expression pattern of EGOT in LUAD, clarify its clinical significance, and explore the molecular mechanism regulating LUAD cell malignant phenotypes. Tumor tissues and their corresponding adjacent normal tissues were obtained from a cohort of 130 LUAD patients. EGOT expression was detected by RT-qPCR. LUAD cell models with EGOT overexpression or knockdown were established. CCK-8, Transwell, and flow cytometry assays were employed to assess LUAD cell phenotypes. Bioinformatics prediction, pull-down, and dual-luciferase reporter assay were performed to authenticate the regulatory axis. EGOT was downregulated in LUAD tissues and cells (A549 and PC9), and low EGOT expression was closely associated with poor prognosis and adverse clinical features (advanced TNM stage, increased tumor size, and lymph node metastasis). EGOT impaired the proliferative, migratory and invasive capacities of LUAD cells, and facilitated their apoptotic process. EGOT targeted miR-1323 to regulate QKI expression, forming the EGOT/miR-1323/QKI axis in A549 and PC9 cells. MiR-1323 reversed EGOT's effect in LUAD cells, which was abolished by QKI overexpression. EGOT may act as a tumor suppressor in LUAD <i>via</i> the miR-1323/QKI axis, providing a potential prognostic biomarker for LUAD.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-16"},"PeriodicalIF":1.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Aptamer-guided therapeutics and diagnostics for metabolic syndrome: Design, delivery, and translation of aptamer-based systems\".","authors":"Ameena Ahmed, Manash Pratim Pathak","doi":"10.1080/15257770.2026.2693505","DOIUrl":"https://doi.org/10.1080/15257770.2026.2693505","url":null,"abstract":"<p><p>Metabolic syndrome (MetS) is a composite cardiometabolic condition defined by visceral obesity, dyslipidemia, hypertension, and insulin resistance, and it promotes the worldwide prevalence of type 2 diabetes and atherosclerotic cardiovascular disease. Nucleic-acid aptamers have developed as programmable recognition ligands that integrate antibody-like specificity with chemical synthesis, modular functionalization, low batch variability, and usually a low immunogenicity. Their translational significance in MetS is not solely in target binding, but in the capacity to establish disease-relevant affinity, stability, and tissue selectivity by advanced SELEX, counter-selection, chemical expansion, truncation, and post-SELEX maturation. Aptamers can function as pathway modulators by inhibiting ligand-receptor interactions, retaining biased receptor conformations, or concentrating therapeutic agents in metabolically compromised tissues. This provides them particularly appealing for targets characterized by receptor recycling, cell-state identification, or inflammatory interactions, rather than by a singular static biomarker. They can serve as sensitive biosensing components in aptasensors for glucose, insulin, glycated proteins, lipids, and inflammatory markers, offering more detailed metabolic phenotyping than single-analyte assays. Recent studies on insulin receptor, PCSK9, adipocyte-, and hepatocyte-targeting aptamers illustrate how aptamers may support both disease-modifying therapy and point-of-care diagnostics in obesity, insulin resistance, MASLD, and dyslipidemia. Nevertheless, matrix-aware validation, serum stability, pharmacokinetic optimization, and proof of superiority over well-known biologics and clinical tests are still necessary for effective translation. Aptamer engineering offers a versatile and mechanistically adaptable framework for precision diagnostics along with targeted intervention in Metabolic syndrome. If these challenges be successfully overcome, aptamers may transition from sophisticated binders to clinically applicable metabolic tools.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-45"},"PeriodicalIF":1.0,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148352740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNAs: promising biomarkers for diagnosis, prognosis and therapy in oral squamous cell carcinoma.","authors":"Atika Dogra, Samson Mani, Yasha Hasija","doi":"10.1080/15257770.2026.2677626","DOIUrl":"https://doi.org/10.1080/15257770.2026.2677626","url":null,"abstract":"<p><p>Circular RNAs (circRNAs) have arisen as a new area of interest among non-coding RNAs in cancer. Increased stability, abundance in human cells, tissue specificity, and regulatory functions in various diseases including cancer indicate that circRNAs may serve as important biomarkers. Oral squamous cell carcinoma (OSCC), the leading form of oral cancer, exhibits decreased survival, high recurrence, and mortality rates, and has been a significant public health concern for a long time. The molecular mechanisms involved in OSCC are not well understood, and there is a significant lack of accurate biomarkers for its timely identification, and prognosis, along with treatment targets. Existing literature suggests that circRNAs are implicated in various aspects in OSCC carcinogenesis and reveal their potential role in the detection and management of disease. The interaction between circRNAs, and miRNAs induces modulation of multiple signaling pathways, and assists in distinct cancer characteristics like generating own growth signals, angiogenesis, infinite proliferation, tissue invasion, and metastasis. This review summarizes protumoal, and antitumoral properties of circRNAs, their role as prospective biomarkers, and therapy targets along with their involvement in signaling pathways impacting OSCC tumorigenesis, and progression.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-25"},"PeriodicalIF":1.3,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148043088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene therapy, RNA-based drugs, and CRISPR in neuroprotection.","authors":"Aleena Taufiq, Sajad Ahmad Dar, Saurabh Singh, Arshad Jawed, Taru Singh","doi":"10.1080/15257770.2026.2662373","DOIUrl":"https://doi.org/10.1080/15257770.2026.2662373","url":null,"abstract":"<p><p>Neurodegenerative diseases are associated with progressive neural malfunction, which is driven by common molecular pathologies that encompass protein aggregations, mitochondrial dysfunction, aberrant RNA metabolism and impaired intracellular clearance. Conventional treatments are largely symptomatic with no treatment of the underlying pathology. Gene therapies, RNA-based therapeutic platforms and CRISPR-based genome-editing technologies provide more targeted methods to regulate the pathological pathways and restore neuronal homeostasis. Nevertheless, these interventions can have transient, reversible or long-term effects instead of a consistent irreversible effect depending on the platform being used. Engineered viral vectors, particularly adeno-associated viruses, enable cell-type-specific and circuit-resolved delivery within the central nervous system. Although constrained by a limited packaging capacity (∼4.7 kb), innovations such as dual-vector systems and capsid engineering are expanding their functional utility. RNA therapeutics, such as antisense oligonucleotides, siRNA/miRNA and synthetic mRNA, provide reversible gene expression regulation, whereas CRISPR can be used to disrupt, correct or regulate the expression of specific genes. Together, these platforms constitute a multifaceted and evolving toolkit for neuroprotection, with the potential to modify disease progression in neurodegenerative disorders. However, most approaches remain at preclinical or early clinical stages, and further validation is required to establish long-term efficacy and safety.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-21"},"PeriodicalIF":1.3,"publicationDate":"2026-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, synthesis, and molecular docking evaluation of novel pyrimidine-thiadiazole glycosides as potential anticancer agents.","authors":"Amira A Ghoneim, Abdullah Y Abdullah Alzahrani, Wassila Derafa, Eiman A A Elshaygi, Ahmed Foud El-Farargy","doi":"10.1080/15257770.2026.2664609","DOIUrl":"https://doi.org/10.1080/15257770.2026.2664609","url":null,"abstract":"<p><p>A series of novel pyrimidine-thiadiazole conjugates incorporating carbohydrate-based 1,3,4-thiadiazole thioglycosides were synthesized and analyzed using (IR), (¹H-NMR) and (¹³C-NMR) spectroscopy. The synthesized compounds were evaluated for their cytotoxic activity against human liver <b>(</b>HepG2<b>),</b> breast <b>(</b>MCF-7<b>),</b> and lung <b>(</b>A549<b>)</b> cancer cell lines. Compound <b>10</b> showed the strongest activity with IC₅₀ values of 10.73 ± 2.04 mM (HepG2), 9.10 ± 1.97 mM (MCF-7), and 9.25 ± 2.94 mM (A549). while <b>6</b> and <b>7</b> demonstrated moderated activity. In contrast, the reference drug doxorubicin exhibited higher potency, with IC₅₀ values of 3.71 ± 1.12 mM (HepG2), 2.68 ± 0.11 mM (MCF-7), and 3.22 ± 0.22 mM (A549). Molecular docking studies revealed compound <b>10</b> had favorable binding profile with an S-score of -6.33 kcal/mol and RMSD of 3.10 Å, forming key interactions including hydrogen bonding with GLU 142, H-acceptor interaction with GLY 104, and π-H interaction with HIS 141. Compound <b>5</b> also exhibited strong binding interactions (S-score = -5.87 kcal/mol) with multiple metal and ionic interactions, whereas compounds <b>6, 7,</b> and <b>11</b> displayed comparatively weaker binding affinities. These results indicate that compound <b>10</b> is the most promising candidate in this series, with both enhanced cytotoxic activity and favorable docking interactions, suggesting that pyrimidine-thiadiazole hybrids represent a valuable scaffold for further anticancer drug development.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-17"},"PeriodicalIF":1.3,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-769-5p inhibits cellular behaviors associated with endometriosis progression by directly targeting follistatin in vitro.","authors":"Yibo Meng, Qinghua Chen, Han Lu","doi":"10.1080/15257770.2026.2657461","DOIUrl":"https://doi.org/10.1080/15257770.2026.2657461","url":null,"abstract":"<p><p>The pathogenesis of endometriosis (EM) remains intricate and multifactorial. This study aimed to investigate the biological functions of microRNA-769-5p (miR-769-5p) in an <i>in vitro</i> model of EM. Serum was obtained from 120 EM patients and 100 healthy controls. miRNA and mRNA expression were quantified by RT-qPCR. Functional analyses of miR-769-5p were performed in vitrousing cell counting kit-8 (CCK-8) cell proliferation assays, Transwell assays, and dual-luciferase assays in an endometriosis-derived stromal cell line (hEM15A). miR-769-5p was markedly down-regulated in EM sera and correlated inversely with disease severity (<i>p</i> < 0.001). Moreover, miR-769-5p exhibited high diagnostic accuracy for EM (AUC = 0.9166, <i>p</i> < 0.001). In the hEM15A cell model, overexpression of miR-769-5p effectively suppressed the proliferation, migration, and invasion capabilities of EM-derived stromal cells (<i>p</i> < 0.001). miR-769-5p targeted follistatin (FST) and negatively regulated its expression <i>in vitro</i> (<i>p</i> < 0.001). In contrast, FST overexpression could partially reverse the inhibitory effects of miR-769-5p-mimic on these EM-derived cells. Our findings indicate that miR-769-5p is downregulated in EM serum. Furthermore, <i>in vitro</i> experiments suggest that it can target FST to inhibit proliferation, migration, and invasion of ectopic endometrial stromal cells.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-19"},"PeriodicalIF":1.3,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147818452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"d-Alanyl:d-alanine ligase inhibitors as antibacterial agents against <i>Staphylococcus aureus</i>: a review.","authors":"Mohammed Afzal Azam, Abisha Thomas, Srikanth Jupudi","doi":"10.1080/15257770.2026.2661075","DOIUrl":"https://doi.org/10.1080/15257770.2026.2661075","url":null,"abstract":"<p><p>Gram-positive pathogen <i>Staphylococcus aureus</i> is associated with human mortality and morbidity worldwide. Mutants of this pathogen is responsible for life threatening community-associated meticillin-resistant infections. Further, the evolution of multidrug-resistant <i>Staphylococcus aureus</i> strains emphasizes the urgent need to develop novel antibacterial agents. ATP-dependent D-alanyl:D-alanine ligase is essential to produce peptidoglycan of bacterial cells, and its inhibition can lead to bacterial cell death, making it a valuable antibacterial target. D-alanyl-D-alanine ligase is recognized as a validated target for the design and development of novel antibacterial agents to overcome resistance problems. In the past several approaches have been used to develop D-alanyl-D-alanine ligase inhibitors with potent antibacterial activity. Some of these inhibitors, including <i>N</i>-acyl-substituted sulfamides, 1-(2-hydroxybenzoyl)-thiosemicarbazide, benzoylthiosemicarbazide, benzoxazoles and diazenedicarboxamides exhibited significant activity against <i>Staphylococcus aureus</i>. The present findings clearly demonstrate that inhibiting D-alanyl:D-alanine ligase is a viable strategy for the discovery of new antibacterial therapies against <i>Staphylococcus aureus</i>.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-21"},"PeriodicalIF":1.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, structural elucidation, DNA binding, cleavage, cholinesterase inhibitory activity of metal complexes of novel 2,2'-bipyridyl derivative.","authors":"Ebenezer Bonpandi, Caroline John, Pechiammal Arumugam","doi":"10.1080/15257770.2026.2659226","DOIUrl":"https://doi.org/10.1080/15257770.2026.2659226","url":null,"abstract":"<p><p>To achieve efficient cholinesterase inhibitory activity of metal(II) complexes of Cu(II), Ni(II), Co(II), and Zn(II) with 2,2'-bipyridyl framework [M-L] (<i>L</i> = 2,2'-bipyridyl derivative containing an aromatic center and an e^--withdrawing -NO₂ group) was developed. The structural characteristics were identified through spectroscopic and analytical studies. The antibacterial activity of the produced ligand and metal(II) complexes against bacteria and fungi was evaluated. The synthesized metal(II) complexes ability to fragment DNA has been studied on pUC 18 DNA using agarose gel electrophoresis. The copper(II) complex (K_b=4.11 × 10⁵ M^-1) is stronger binding affinity for DNA than ethidium bromide (EB) (K_b=3.3 × 10⁵ M^-1) and metal(II) complexes. The chemically produced 2,2'-bipyridyl derivative had the strongest inhibitory effects against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) with IC₅₀ values that were less than the standard compounds (0.34 and 3.42 µM, respectively). Our research results could aid in the creation of novel drug molecules, especially for the treatment of neurological conditions like Alzheimer's disease and neurological disorders occurring through diabetes.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-34"},"PeriodicalIF":1.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-1247-5p is correlated with cervical cancer and regulates cervical cancer cell functions by targeting DVL1.","authors":"Yuhua Yan, Jing Li, Cuiya Zhang, Ruiying Yang, Mengzhen Xie, Jing Zhang","doi":"10.1080/15257770.2026.2655701","DOIUrl":"https://doi.org/10.1080/15257770.2026.2655701","url":null,"abstract":"<p><p>The incidence of cervical cancer (CC) is extremely high, yet current diagnostic biomarkers have not achieved satisfactory results. MicroRNAs can regulate vital processes in tumors, including proliferation and apoptosis. The regulatory role of miR-1247-5p in CC remains unknown. This study aims to investigate the diagnostic value of miR-1247-5p in CC. This study included 156 patients with CC and 130 healthy volunteers. Real-time quantitative PCR was used to detect miR-1247-5p and Dishevelled 1 (DVL1) levels. Cell proliferation was assessed using the CCK-8 assay. Levels of Fe2+, lipid reactive oxygen species, malondialdehyde, and glutathione were measured using commercial kits. Diagnostic value of each biomarker was evaluated via receiver operating characteristic curve analysis. miR-1247-5p is significantly downregulated in CC. Low miR-1247-5p constitutes one of the risk factors for CC development. miR-1247-5p mimic inhibits proliferation and promotes ferroptosis. DVL1 is a target of miR-1247-5p. It is upregulated in both serum from CC patients and CC cells. oe-DVL1 partially reverses the suppression of CC malignant behavior by miR-1247-5p mimic. Therefore, we conclude that miR-1247-5p suppresses the malignant behavior of CC by targeting DVL1 and may serve as a potential clinical diagnostic biomarker.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-15"},"PeriodicalIF":1.3,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating multi-encoding sequence features via stacking ensemble learning for RNA m5C site prediction.","authors":"Ubaid Ur Rahman, Naeem Ul Islam","doi":"10.1080/15257770.2026.2658190","DOIUrl":"https://doi.org/10.1080/15257770.2026.2658190","url":null,"abstract":"<p><p>RNA 5-methylcytosine (m5C) is an important epitranscriptomic modification involved in RNA stability, translation, and post-transcriptional regulation. Accurate identification of m5C sites remains challenging due to limited sequence representation and insufficient feature integration in existing computational methods. In this study, we propose a comprehensive machine learning framework that integrates six complementary sequence encoding schemes, including enhanced nucleic acid composition (ENAC), tri-nucleotide composition (TNC), composition of K-spaced nucleic acid pairs (CKSNAP), pseudo-electron-ion interaction potential (PseEIIP), one-hot encoding, and nucleotide chemical properties (NCP). Each encoding is paired with an optimal classifier, and a stacking ensemble strategy is employed to fuze the outputs of base classifiers. The model is trained using 5-fold cross-validation for base learners and 3-fold cross-validation for the meta-learner. Performance evaluation using multiple metrics demonstrates that the proposed approach achieves improved robustness and cross-dataset generalization, with an accuracy of 75.5%, MCC of 0.51, and PR-AUC of 0.82. These results indicate that the proposed fusion-based ensemble framework provides an effective and reliable solution for RNA m5C site prediction.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-29"},"PeriodicalIF":1.3,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}