Nucleosides, Nucleotides & Nucleic Acids最新文献_第3页

Effect of single strand breaks on the formation of DNA interstrand crosslinks induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine. 单链断裂对主要氧化腺嘌呤损伤诱导的DNA链间交联形成的影响7,8-二氢-8-氧腺嘌呤。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-12 DOI: 10.1080/15257770.2025.2529353

Aaron L Rozelle, Louis B Zhang, Seongmin Lee

{"title":"Effect of single strand breaks on the formation of DNA interstrand crosslinks induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine.","authors":"Aaron L Rozelle, Louis B Zhang, Seongmin Lee","doi":"10.1080/15257770.2025.2529353","DOIUrl":"https://doi.org/10.1080/15257770.2025.2529353","url":null,"abstract":"The major oxidative adenine lesion, 7,8-dihydro-8-oxoadenine (oxoA), can readily undergo further oxidation to generate the highly genotoxic DNA interstrand cross-links (ICLs). Herein we report that the presence of single-strand breaks (SSBs), the major lesion formed at sites of oxidative stress, in the form of a nick or single-nucleotide gap in the phosphodiester backbone of duplex DNA significantly increases the cross-linking yield of oxoA with all canonical nucleotides (up to 67.5%) upon oxidation. The cross-linking reaction occurs between the purine/pyrimidine moiety of a nucleotide on the complementary strand of the duplex and the oxoA modification on the template strand, which was confirmed by the experiment involving the use of 2',3'-dideoxycytosine. Interestingly, the minor cross-linking products in intact DNA saw a more significant increase in reactivity relative to the major oxoA-G ICL. SSBs in the form of a gap or nick between the reacting nucleotide and thymine residue base paired to oxoA produced the most significant increase in yield.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-19"},"PeriodicalIF":1.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of CpG site of MTHFR gene promoter and type 2 diabetes in Moroccan population susceptibility. 摩洛哥人群MTHFR基因启动子CpG位点与2型糖尿病易感性的关系

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-10 DOI: 10.1080/15257770.2025.2532089

Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Nouha Messaoudi, Sofia Sehli, Najib Al Idrissi, Omar Abidi, Wajih Rhalem, Naima Khlil, Hassan Ghazal, Salsabil Hamdi

{"title":"Association of CpG site of MTHFR gene promoter and type 2 diabetes in Moroccan population susceptibility.","authors":"Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Nouha Messaoudi, Sofia Sehli, Najib Al Idrissi, Omar Abidi, Wajih Rhalem, Naima Khlil, Hassan Ghazal, Salsabil Hamdi","doi":"10.1080/15257770.2025.2532089","DOIUrl":"https://doi.org/10.1080/15257770.2025.2532089","url":null,"abstract":"Type 2 diabetes (T2D) is a complex multifactorial metabolic disorder characterized by progressive disease progression, involving varying degrees of insulin resistance and pancreatic islet dysfunction. Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme regulating folate metabolism, and its polymorphisms have been associated with T2D. However, the methylation pattern of the MTHFR gene has not been previously studied. This study aimed to assess the association between T2D and the methylation profile of the MTHFR gene promotor in a Moroccan population. A total of 107 patients with T2D and 100 healthy controls were included in the study. The methylation status of CpG sites in the MTHFR gene promoter was conducted by methylation-specific PCR (MS-PCR). Statistical analyses were performed using SPSS software (version 20). The promoter region of the MTHFR gene was predominantly hyper-methylated in patients with T2D compared to healthy controls (OR: 2.924; 95% CI: 1.285-6.650; p = 0.008). The hypermethylated profile was not influenced by environmental or metabolic factors examined in this study. These findings suggest that hypermethylation of CpG sites in the MTHFR gene promoter is associated with T2D in the Moroccan population, highlighting a potential epigenetic mechanism contributing to the disease.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-320a expression shows correlation with viral load in EBV-negative Burkitt lymphoma samples. 在ebv阴性伯基特淋巴瘤样本中，MiR-320a的表达与病毒载量相关。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-07-03 DOI: 10.1080/15257770.2025.2527141

Mevsim Saydam, Sercan Ergün, Ayhan Dağdemir, İbrahim Kartal, Oğuz Salih Dinçer, Seda Gün, Özlem Terzi

{"title":"MiR-320a expression shows correlation with viral load in EBV-negative Burkitt lymphoma samples.","authors":"Mevsim Saydam, Sercan Ergün, Ayhan Dağdemir, İbrahim Kartal, Oğuz Salih Dinçer, Seda Gün, Özlem Terzi","doi":"10.1080/15257770.2025.2527141","DOIUrl":"https://doi.org/10.1080/15257770.2025.2527141","url":null,"abstract":"The Epstein-Barr virus (EBV) infection status varies among BL subtypes. There are unresolved questions regarding the contribution of EBV, which is strongly associated with Burkitt lymphoma, to BL pathogenesis. Differences between EBV-positive and EBV-negative BL have been previously reported. A long-debated and studied topic is the differing origins of EBV-positive and EBV-negative BL cells. Studies have suggested that miRNAs, which are post-transcriptional elements involved in many pathways, play a role in this process. In our study, in silico analyses and a literature review were used to identify miRNAs potentially involved in lymphoma and B lymphocyte development pathways. Three miRNAs (miR-182, miR-320a, miR-144) targeting the BLIMP1 and XBP1 genes, which act as regulators in B lymphocyte development, and associated with lymphoma were identified. Paraffin-embedded tissue samples from 28 patients diagnosed with BL were included in the study. Real-Time PCR analysis of the BamHI-W and ApoB regions classified the samples into EBV-positive and EBV-negative groups. Expression analysis of the selected three miRNAs was performed using Real-Time PCR. Expression of miR-320a was observed in all samples, and a positive correlation was found between miR-320a expression and the viral load in the EBV-negative group. This study aimed to gain insights into the role of EBV in BL pathogenesis and has demonstrated the potential role of miR-320a. In conclusion, our study demonstrates that specific miRNAs are significantly associated with EBV in Burkitt lymphoma. These findings highlight the potential of targeting these miRNAs for therapeutic interventions and suggest further research into their mechanisms of action.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-18"},"PeriodicalIF":1.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boronic acid-modified nucleoside - synthesis and structural characterisation in the solid state. 硼酸修饰核苷的合成及固态结构表征。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-28 DOI: 10.1080/15257770.2025.2512848

Tabea Lenz, Marian Hebenbrock

引用次数: 0

LINC00365 serves as a prognostic biomarker for thyroid cancer and regulates cell proliferation, migration and invasion by targeting miR-485-5p. LINC00365作为甲状腺癌的预后生物标志物，通过靶向miR-485-5p调控细胞增殖、迁移和侵袭。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-26 DOI: 10.1080/15257770.2025.2522350

Henghua Xiao, Manlong Long, Jun Yang, Qiong Luo, Lingli Hu, Fang Chen

{"title":"LINC00365 serves as a prognostic biomarker for thyroid cancer and regulates cell proliferation, migration and invasion by targeting miR-485-5p.","authors":"Henghua Xiao, Manlong Long, Jun Yang, Qiong Luo, Lingli Hu, Fang Chen","doi":"10.1080/15257770.2025.2522350","DOIUrl":"https://doi.org/10.1080/15257770.2025.2522350","url":null,"abstract":"Thyroid cancer (TC) is the most prevalent form of malignancy within the endocrine system globally, and its incidence has been increasing significantly in recent years. The aim of this study was to explore the clinical role of LINC00365 in TC, and to analyze the regulatory mechanism between LINC00365 and miR-485-5p and its specific impact on TC. This study included 135 TC patients. The levels of LINC00365 in TC tissues and cells were detected by RT-qPCR. The impact of LINC00365 on TC prognosis was investigated using Kaplan-Meier curve and multivariate Cox regression analysis. Furthermore, the impact of LINC00365 on TC cell proliferation was examined via CCK-8 assay, while transwell assays were utilized to evaluate changes in migration and invasion capabilities. The regulatory interaction between LINC00365 and miR-485-5p was confirmed through dual-luciferase reporter assay. The expression of LINC00365 was upregulated in TC tissues and cells, and its high expression was closely related to the poor prognosis of TC patients. Kaplan-Meier curve revealed a notable correlation between elevated LINC00365 expression levels and reduced survival outcomes and multivariate Cox regression analysis revealed that elevated LINC00365 expression levels served as an independent prognostic indicator, significantly associated with adverse clinical outcomes in TC patients. In addition, LINC00365 negatively regulated the expression level of miR-485-5p. Inhibiting LINC00365 may suppress the proliferation, migration and invasion abilities of TC cells. LINC00365 may be a potential prognostic indicator for TC, and LINC00365 may promote tumor growth and metastasis of TC by inhibiting miR-485-5p expression.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-14"},"PeriodicalIF":1.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral analysis of protonated and deprotonated form of ribonucleotides and their components at room temperature. 室温下核糖核苷酸的质子化和去质子化形式及其组分的光谱分析。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-26 DOI: 10.1080/15257770.2025.2521555

Mykhailo Dotsenko, Roman Nikolaiev, Zenovii Tkachuk

引用次数: 0

Aptamer-directed siRNA delivery systems for triple-negative breast cancer therapy. 适配体定向siRNA递送系统用于三阴性乳腺癌治疗。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-26 DOI: 10.1080/15257770.2025.2524468

Dilpreet Singh, Satvir Singh, Nitin Tandon

{"title":"Aptamer-directed siRNA delivery systems for triple-negative breast cancer therapy.","authors":"Dilpreet Singh, Satvir Singh, Nitin Tandon","doi":"10.1080/15257770.2025.2524468","DOIUrl":"https://doi.org/10.1080/15257770.2025.2524468","url":null,"abstract":"Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer characterized by the absence of estrogen, progesterone, and HER2 receptors, making it unresponsive to targeted hormonal and HER2-based therapies. Current treatment options, including chemotherapy and radiation, have limited efficacy and are associated with severe side effects, emphasizing the need for innovative therapeutic strategies. Aptamer-siRNA conjugates have emerged as a promising gene-silencing approach, leveraging the high specificity of nucleic acid aptamers to selectively deliver short interfering RNA (siRNA) to TNBC cells. Aptamers, single-stranded DNA or RNA molecules generated via SELEX, exhibit nanomolar-range binding affinities (Kd ∼0.5-2.5 nM) for TNBC biomarkers such as EGFR, EpCAM, nucleolin, and MUC1, enabling receptor-mediated internalization of siRNA. Preclinical studies have demonstrated that aptamer-siRNA conjugates enhance cellular uptake by 5-10-fold, improve gene silencing efficiency (80-95%), and extend siRNA stability in circulation (from <2 h to 6-9 h). In xenograft models, aptamer-siRNA therapies have shown tumor volume reductions of 60-85%, outperforming non-targeted siRNA and chemotherapy. However, challenges such as nuclease degradation, immune responses, endosomal escape, and large-scale production remain significant hurdles to clinical translation. Recent advances in chemical modifications, lipid-based carriers, and artificial intelligence-driven aptamer design are addressing these limitations, paving the way for personalized, precision RNAi-based therapeutics. This review explores the mechanisms, recent advancements, challenges, and future directions of aptamer-siRNA therapeutics, providing a comprehensive analysis of their potential to revolutionize TNBC treatment by offering targeted, effective, and less toxic gene-silencing approaches.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-19"},"PeriodicalIF":1.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct regulation mechanism of immune-related genes in left-sided and right-sided colon cancer. 免疫相关基因在左右两侧结肠癌中的不同调控机制。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-23 DOI: 10.1080/15257770.2025.2521554

Tianfei Yi, Qi Liao, Zhiqin Jiang

{"title":"Distinct regulation mechanism of immune-related genes in left-sided and right-sided colon cancer.","authors":"Tianfei Yi, Qi Liao, Zhiqin Jiang","doi":"10.1080/15257770.2025.2521554","DOIUrl":"https://doi.org/10.1080/15257770.2025.2521554","url":null,"abstract":"Colon cancer is one of the most common cancers in worldwide. Emerging evidence has demonstrated distinct patterns of immune infiltration between left colon cancer (LCC) and right colon cancer (RCC), classified according to primary tumor location. However, the underlying mechanism was still unknown. Here, we identified 111 more up-regulated genes (MURGs) including PD1 (PDCD1) and CTLA4 in RCC and 166 more down-regulated genes (MDRGs) in LCC, all participating in immune-related biological processes. Notably, CD83, CXCR4 and ISL1 emerged as reversely regulated immune-related genes (IRGs) showing opposite regulation patterns between the two cancer types. Through regulatory network analysis, we found that genetic mutations predominantly drive enhanced immune infiltration in RCC through IRG up-regulation, whereas DNA methylation-mediated IRG suppression accounts for diminished immune responses in LCC. Furthermore, prognostic models based on these IRGs with high-quality were constructed in LCC and RCC respectively. In conclusion, our results provide crucial insights into the divergent immunoregulatory mechanisms governing LCC and RCC, potentially facilitating the discovery of novel biomarkers for prognosis prediction and targeted therapy.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-28"},"PeriodicalIF":1.1,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-922 alleviates LPS-induced HK-2 cell apoptosis and inflammation through TGF-β/Smad. MiR-922通过TGF-β/Smad缓解lps诱导的HK-2细胞凋亡和炎症。

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-22 DOI: 10.1080/15257770.2025.2518554

Bo Yang, Li Xu, Peng Zhou, Qihao Ma, Yong Li, Danan Sun

{"title":"MiR-922 alleviates LPS-induced HK-2 cell apoptosis and inflammation through TGF-β/Smad.","authors":"Bo Yang, Li Xu, Peng Zhou, Qihao Ma, Yong Li, Danan Sun","doi":"10.1080/15257770.2025.2518554","DOIUrl":"10.1080/15257770.2025.2518554","url":null,"abstract":"Background: MicroRNAs play an extensive role in human chronic kidney diseases (CKD), but the role of miR-922 remains unclear.Purpose: This study was designed to investigate the potential mechanism by which miR-922 acts in CKD.Methods: The human proximal renal tubular cell line (HK-2) was treated by LPS to build a CKD cell model. The expressions of miR-922, TGFβR1, and p-Smad2/3 were detected by RT-qPCR or western blotting. The dual-luciferase reporter assay verified the interaction of miR-922 with TGFβR1. Cell viability and apoptosis were assessed using the CCK-8 assay and Annexin V-FITC/PI method. An ELISA kit was used for the measure of TNF-α/IL-1β levels.Results: MiR-922 was scanty in HK-2 cells with LPS treatment. MiR-922 was an upstream regulator of TGFβR1. The up-regulation of miR-922 restrained TGFβR1 expression, thereby hindering the activation of Smad2/3. Overexpression of miR-922 and inhibition of Smad2/3 alleviated the LPS-induced apoptosis and inflammation, while TGFβR1 played a pro-apoptotic and pro-inflammatory role.Conclusion: MiR-922 rescued HK-2 cell apoptosis and inflammation damage induced by LPS through negative regulation of TGF-β/Smad pathway, which may be a vital molecular mechanism of CKD.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-11"},"PeriodicalIF":1.1,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of lung squamous cell carcinoma subtypes based on STING pathway expression and validation of prognostic features. 基于STING通路表达的肺鳞状细胞癌亚型鉴定及预后特征验证

IF 1.1 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-14 DOI: 10.1080/15257770.2025.2505467

Wenjun Liu, Dezhong Cheng

{"title":"Identification of lung squamous cell carcinoma subtypes based on STING pathway expression and validation of prognostic features.","authors":"Wenjun Liu, Dezhong Cheng","doi":"10.1080/15257770.2025.2505467","DOIUrl":"https://doi.org/10.1080/15257770.2025.2505467","url":null,"abstract":"Lung squamous cell carcinoma (LUSC), a prevalent non-small cell lung cancer subtype, demonstrates marked heterogeneity and unpredictable prognosis. This study established a prognostic model using STING pathway-related genes to stratify LUSC patients and guide immunotherapy. Through weighted gene co-expression network analysis of TCGA-LUSC data, we identified the MEbrown module containing 13 STING-associated key genes (including CD47 and CLDN5) to develop the STING Pathway Death-Related Signature (SPDRS). LASSO regression refined the model, which effectively stratified patients into distinct high- and low-risk groups with significant survival differences. High-risk patients exhibited enhanced immune infiltration, particularly T cells CD4 memory resting and M2 macrophages, along with elevated immune checkpoint expression and stromal scores. Functional analyses revealed enrichment in immune-related pathways and tumor microenvironment regulation. Drug sensitivity predictions identified potential therapeutic agents targeting SPDRS components. A nomogram integrating SPDRS with clinical factors demonstrated strong prognostic accuracy. This work provides a novel STING pathway-based stratification system that elucidates tumor microenvironment heterogeneity and informs personalized treatment strategies. The findings highlight SPDRS as both a prognostic biomarker and therapeutic response predictor, advancing precision immunotherapy in LUSC management.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-18"},"PeriodicalIF":1.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0