Nucleosides, Nucleotides & Nucleic Acids最新文献_第3页

Research on correlations of miR-374a-5p expression with progression and prognosis of prostate cancer. miR-374a-5p表达与前列腺癌进展及预后的相关性研究

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-23 DOI: 10.1080/15257770.2025.2481947

Ke Lv, Haiyan Pan, Hui Yao

{"title":"Research on correlations of miR-374a-5p expression with progression and prognosis of prostate cancer.","authors":"Ke Lv, Haiyan Pan, Hui Yao","doi":"10.1080/15257770.2025.2481947","DOIUrl":"10.1080/15257770.2025.2481947","url":null,"abstract":"Prostate cancer (PCa) is a frequently occurring malignant tumor affecting male reproductive system. miR-374a-5p was identified to participate in regulation of several tumors. The aim of the research was to discuss the influence for miR-374a-5p upon PCa progression and prognosis. A total of 112 PCa and 110 benign prostatic hyperplasia tissue samples were collected for the study. Real-time quantitative polymerase chain reaction was adopted to examine miR-374a-5p level in PCa tissues and cells. Kaplan-Meier and Cox model were applied to evaluate prognostic significance of miR-374a-5p for PCa. CCK8 and Transwell assays were carried out to analyze the efficacy of miR-374a-5p in PCa cell proliferation, migration and invasion. miR-374a-5p was under-expressed in PCa tissues and cells. Low expression of miR-374a-5p is linked to less favorable prognosis in PCa sufferers. Additionally, Cox analysis revealed that miR-374a-5p and TNM stage were two independent prognostic factors for PCa. Cellular assays showed that upregulating miR-374a-5p suppressed PCa cell proliferation, migration, and invasion.Conversely, knockdown of miR-374a-5p facilitated PCa cell proliferation, migration, and invasion. miR-374a-5p expression decreased in PCa and was remarkably related to poor prognosis in PCa patients. miR-374a-5p acts in PCa by inhibiting cell proliferation, migration, and invasion. Consequently, miR-374a-5p has potential to act as a prognostic biomarker and a target for clinical therapeutic intervention in PCa.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"426-437"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-103 promotes esophageal squamous cell carcinoma metastasis by targeting FOXP1. miR-103 通过靶向 FOXP1 促进食管鳞状细胞癌转移

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-21 DOI: 10.1080/15257770.2025.2478980

Min Huang, Jun Cai, Hai Zeng, Yan Zhu, Fan Zhang, Shuang Li

{"title":"miR-103 promotes esophageal squamous cell carcinoma metastasis by targeting FOXP1.","authors":"Min Huang, Jun Cai, Hai Zeng, Yan Zhu, Fan Zhang, Shuang Li","doi":"10.1080/15257770.2025.2478980","DOIUrl":"10.1080/15257770.2025.2478980","url":null,"abstract":"Esophageal squamous cell carcinoma (ESCC), a prevalent malignancy within the digestive tract, is associated with a significantly high mortality rate. MicroRNAs were already demonstrated to work in a wide range of tumors. The objective of the present research was to elucidate the involvement of miR-103 in the pathogenesis of ESCC and to explore its underlying mechanisms of action. Real-time quantitative polymerase chain reaction was used to detect miR-103 expressions in ESCC tissues and cells. The clinical significance of these expressions was assessed by a series of statistical analyses. Transwell assay was used to study the impact of miR-103 on migration and invasion ability of ESCC cells. Furthermore, a dual luciferase reporter gene method was adopted to study the association of miR-103 with the targeting of forkhead box protein 1 (FOXP1). miR-103 was significantly up-regulation in ESCC tissues and cell lines. Clinically, high miR-103 expression was associated with negative prognosis in ESCC. The low miR-103 expression significantly inhibited cell proliferation, migration and invasion in ESCC cell lines. Furthermore, miR-103 regulated the mechanism of action of ESCC by targeting FOXP1. In this study, we found that miR-103 may serve as a biomarker for ESCC prognosis. miR-103 may promote ESCC cell metastasis by targeting FOXP1. These studies may elucidate the potential of miR-103 as a novel target for the treatment of ESCC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"50-63"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 7436-bp mitochondrial DNA deletion as a risk factor for ulcerative colitis in the Iranian population. 7436 bp线粒体DNA缺失是伊朗人群溃疡性结肠炎的危险因素。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-25 DOI: 10.1080/15257770.2025.2484317

Rasoul Zahmatkesh Roodsari, Zivar Salehi, Kazem Parivar, Farhad Mashayekhi, Keyvan Aminian

{"title":"The 7436-bp mitochondrial DNA deletion as a risk factor for ulcerative colitis in the Iranian population.","authors":"Rasoul Zahmatkesh Roodsari, Zivar Salehi, Kazem Parivar, Farhad Mashayekhi, Keyvan Aminian","doi":"10.1080/15257770.2025.2484317","DOIUrl":"10.1080/15257770.2025.2484317","url":null,"abstract":"Ulcerative colitis (UC) is a chronic condition characterized by inflammation in the colon. Free radicals and oxidative stress play a significant role in the pathophysiology of UC. Excessive production of reactive oxygen species can damage the mitochondrial genome, leading to mutations such as the7436-bp deletion. The aim of this study was to identify the presence of the 7436-bp mtDNA deletion in patients with UC and its association with susceptibility to colon inflammation. This case-control study, included 195 patients with UC and 250 healthy individuals from the Iranian population. The Multiplex PCR method was used to detect the 7436-bp mtDNA deletion. Statistical analysis was performed using SPSS software. The frequency of 7436-bp mtDNA deletion in patients was 41.5% and 6.8% in healthy individuals. Statistical analysis showed a significant association between the frequency of the 7436-bp mtDNA deletion and UC (p = 0.016). Furthermore, a significant difference was found between the presence of this deletion and an increased risk of severe (p = 0.003) and extensive (p = 0.002) forms of UC. There was no statistically significant difference in the frequency of this deletion between younger patients and the control group. This study suggests that the presence of the 7436-bp mtDNA deletion is a risk factor for UC and plays a significant role in the pathogenesis of the disease. Further research involving larger and more diverse populations is necessary to validate or challenge these findings. Identifying these mutations can enhance our understanding of genetic factors influencing UC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"353-363"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the expression levels of MEFV gene in patients with frequent MEFV pathogenic variants in Kahramanmaras (Turkey). 土耳其Kahramanmaras地区MEFV致病性变异体患者MEFV基因表达水平的研究

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-05-28 DOI: 10.1080/15257770.2025.2511104

Eda Ganiyusufoglu, Hasan Daglı, Metin Kılınc

{"title":"Investigation of the expression levels of MEFV gene in patients with frequent MEFV pathogenic variants in Kahramanmaras (Turkey).","authors":"Eda Ganiyusufoglu, Hasan Daglı, Metin Kılınc","doi":"10.1080/15257770.2025.2511104","DOIUrl":"10.1080/15257770.2025.2511104","url":null,"abstract":"The main objective of this study is to detect the variants in patients who were diagnosed with familial Mediterranean fever (FMF) according to Tel-Hashomer diagnostic criteria and investigated the relationship between genotype-phenotype and the gene expression levels of the Mediterranean fever (MEFV) gene. Variant screening was achieved by automated sanger sequencing, and expression levels of the MEFV gene were analyzed by quantitative real time polymerase chain reaction (RT-PCR). A total of 46 patients with MEFV gene pathogenic variants and 8 control individuals without any variants were enrolled in the study. The most commonly encountered variants in heterozygote genotype were M694V (n = 4), E148Q (n = 3), and M680I (n = 2); in compound heterozygote genotype were M694V/R202Q (n = 4), and R202Q/E148Q (n = 3); in complex heterozygote genotype were R202Q/M694V/M680I (n = 4) and R202Q/M694V/V726A (n = 3); in homozygote genotype were M680I/M680I (n = 7) and M694V/M694V (n = 4). The gene expression levels of the patients with homozygous variants were found to be significantly lower than the healthy control group and patients with heterozygous variants. In patients with M694V homozygous variants, where clinical manifestations are severe, a remarkable decrease in the gene expression of the MEFV gene was observed. It was detected that there was a relationship between the genotype and gene expression level and that the level of gene expression and clinical symptoms were inversely correlated in patients with FMF.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"392-405"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic value of miR-378c in hepatocellular carcinoma and its regulatory effect on tumor progression. miR-378c在肝癌中的预后价值及其对肿瘤进展的调控作用。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-26 DOI: 10.1080/15257770.2025.2481950

Yuanjie Bao, Haoxiang Zhu

{"title":"Prognostic value of miR-378c in hepatocellular carcinoma and its regulatory effect on tumor progression.","authors":"Yuanjie Bao, Haoxiang Zhu","doi":"10.1080/15257770.2025.2481950","DOIUrl":"10.1080/15257770.2025.2481950","url":null,"abstract":"Objective: This study aimed to explore the diagnostic and prognostic value of miR-378c in hepatocellular carcinoma (HCC) patients.Methods: This study included 97 HCC patients, 84 cirrhosis patients and 80 healthy volunteers. Serum miR-378c of all subjects and HCC cell lines was detected by qRT-PCR, and ROC curves were plotted to assess the clinical diagnostic value of miR-378c for HCC. The prognostic performance of miR-378c in HCC was assessed using the Kaplan-Meyer method and COX regression analysis. CCK-8 test for proliferation of HCC cell lines. The migration and invasion of HCC cell lines were measured by Transwell assay. Bioinformatics analysis was employed to analyze the possible target genes of miR-378c.Results: Serum miR-378c were remarkably lower in HCC patients than in cirrhosis patients and healthy controls (p < 0.001). ROC curves indicated that serum miR-378c could effectively distinguish HCC patients from healthy controls and cirrhotic patients. Among HCC patients, those with high miR-378c expression had higher cumulative survival (p = 0.001), and COX analysis identified miR-378c as an independent prognostic biomarker for HCC. Overexpression of miR-378c significantly inhibited the proliferation, migration and invasion of MHCC97H and HepG2 cells (p < 0.01). Bioinformatics analysis of miR-378c target genes revealed that miR-378c target genes were enriched in tumor-associated pathways.Conclusion: Serum miR-378c expression is decreased in HCC patients and strongly connected with poor prognosis. As a potential diagnostic and prognostic biomarker for HCC patients, it may provide new insights into the diagnosis and prognosis of HCC.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"438-452"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precise control model of the epidemic: a cross-sectional study. 流行病的精确控制模型：横断面研究。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-03 DOI: 10.1080/15257770.2025.2470736

Mingyun Jiang, Ruiqi Liu, Weiping Yao, Shuang Li, Xiaodong Liang, Haibo Zhang

{"title":"Precise control model of the epidemic: a cross-sectional study.","authors":"Mingyun Jiang, Ruiqi Liu, Weiping Yao, Shuang Li, Xiaodong Liang, Haibo Zhang","doi":"10.1080/15257770.2025.2470736","DOIUrl":"10.1080/15257770.2025.2470736","url":null,"abstract":"Objectives: Coronavirus disease 2019 (COVID-19) is the most influential public health emergency worldwide. Controlling viral infection with people's health and reducing the impact on people's freedom is difficult at present. The precise control of COVID-19 in a city may be a suitable solution.Methods: An anonymous cross-sectional survey was conducted among Hangzhou people between 1 January and 28 February 2022. We organized the classification, incidence rate and mortality of COVID-19. And we introduced the discovery process of Omicron, health code of four colors, the epidemiological investigation and the policies of government in Hangzhou. This paper discusses various measures taken against Omicron in Hangzhou, China, which are effective methods to deal with such public health emergencies.Conclusions: Hangzhou quickly controlled the epidemic through precise control. As of February 1, Hangzhou had 115 confirmed cases with total population is 12.204 million. The rate of severe and death is 0%. Hangzhou's new model of precise control provides an important reference for the global city's response to COVID-19 and the reduction in losses caused by COVID-19. The COVID-19 Omicron variant outbreak indicates that people will still face unpredictable health risks in the future. Precise control is one of the best ways to effectively manage an epidemic, minimize its severity, and reduce losses in all aspects.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"575-588"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of ACE I/D and ATIR A1166C variants in patients with diabetes mellitus with and without peripheral neuropathy in Turkish patients. 土耳其伴有和不伴有周围神经病变的糖尿病患者中ACE I/D和ATIR A1166C变异的评估

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-01-17 DOI: 10.1080/15257770.2025.2451382

Payam Amiri Dashatan, Huseyin Soylu, Mehmet Elbistan, Aysegul Atmaca, Adem Keskin, Zulfinaz Betul Celik, Serbulent Yigit

{"title":"Evaluation of ACE I/D and ATIR A1166C variants in patients with diabetes mellitus with and without peripheral neuropathy in Turkish patients.","authors":"Payam Amiri Dashatan, Huseyin Soylu, Mehmet Elbistan, Aysegul Atmaca, Adem Keskin, Zulfinaz Betul Celik, Serbulent Yigit","doi":"10.1080/15257770.2025.2451382","DOIUrl":"10.1080/15257770.2025.2451382","url":null,"abstract":"Objective: Type 2 Diabetes Mellitus (T2DM) can lead to long-term vascular complications such as diabetic peripheral neuropathy (DPN). This study aimed to investigate the role of angiotensin-converting enzyme (ACE) insertion (I)/deletion (D) and angiotensin II type 1 receptor (AT1R) A1166C variants in the predisposition to T2DM in the Turkish population and their association with DPN.Methods: The study included 90 T2DM patients (42 with DPN) and 50 healthy individuals. ACE I/D and ATIR A1166C gene regions were analyzed for the variant. Both the general genotype distribution of these variants and the observed genotype ratios were examined separately.Results: In the T2DM group, the proportion of individuals with the AA genotype of the AT1R A1166C variant was lower than in the control group, and the proportion of individuals with the AC genotype was higher. There was no significant difference in the genotype distribution between the groups for the ACE I/D variant. There was no significant difference in the genotype distribution of the ACE I/D and ATIR A1166C variants in patients with and without DPN.Conclusion: In the Turkish population, no significant difference was observed in the overall genotype distribution of ACE I/D and AT1R A1166C variants between T2DM patients and healthy individuals, whereas the AC genotype of the AT1R A1166C variant was more frequent in T2DM patients, and the AA genotype was less frequent. For both variants, no significant difference was observed in the genotype distribution between T2DM patients with and without DPN.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"103-112"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of MBL2 gene polymorphisms with type 2 diabetes and its complications in Moroccan population. 摩洛哥人群中MBL2基因多态性与2型糖尿病及其并发症的关系

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-02-17 DOI: 10.1080/15257770.2025.2466429

Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Wajih Rhalem, Lahcen Wakrim, Imane Ettaki, Hassan Ghazal, Najib Al Idrissi, Omar Abidi, Fadel Bakkali, Abderrahim Naamane, Naima Khlil, Salsabil Hamdi

{"title":"Association of MBL2 gene polymorphisms with type 2 diabetes and its complications in Moroccan population.","authors":"Houda El Alami, Meryem Bouqdayr, Khaoula Errafii, Wajih Rhalem, Lahcen Wakrim, Imane Ettaki, Hassan Ghazal, Najib Al Idrissi, Omar Abidi, Fadel Bakkali, Abderrahim Naamane, Naima Khlil, Salsabil Hamdi","doi":"10.1080/15257770.2025.2466429","DOIUrl":"10.1080/15257770.2025.2466429","url":null,"abstract":"The MBL2 gene encodes the mannose-binding lectin protein (MBL), which is secreted by the liver. Several variants of MBL2 have been found to be associated with altered serum levels and susceptibility to various chronic diseases. Defects in MBL protein polymerization that result in functional impairments and/or low serum levels may influence genetic susceptibility to type 2 diabetes (T2D) and its complications. Therefore, the present case-control study was conducted to assess the potential association of six MBL2 gene variants and haplotypes with susceptibility to T2D and its complications in Morocco. The MBL2 gene was genotyped by PCR-sequencing for the promoting, non-coding, and coding regions in 435 individuals. Our findings revealed a significant association between the heterozygous CG and homozygous recessive GG genotypes of the variant at position -221 C > G in the MBL2 gene promoter with an increased risk of T2D. Similarly, for +4 C > T in the non-coding region, statistical analysis indicates a strong association with T2D risk, particularly with the heterozygous CT and homozygous recessive TT genotypes. The LYQC haplotype is also found to be associated with T2D risk. Furthermore, the heterozygous CT genotype, and recessive T allele of the variant at position +4 C > T, and heterozygous GA genotype of codon Gly54Asp of the MBL2 gene, are associated with protection against hypertension in T2D patients. However, no association was observed between MBL2 variants and dyslipidemia in T2D patients. The study concludes that -221 C > G and +4 C > T variants of the MBL2 gene significantly contribute to T2D susceptibility in Morocco.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"131-152"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable synthesis of benzimidazole-based Schiff base using reusable CaAl₂O₄ nanophosphors catalyst: Insights into metal(II) complexes and DNA interactions. 使用可重复使用的CaAl2O4纳米磷光催化剂可持续合成苯并咪唑基希夫碱：金属（II）配合物和DNA相互作用的见解。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-01-19 DOI: 10.1080/15257770.2025.2451375

M Manjunath, F H Sujata, A H Shridhara, B Vinay Kumar, K Prashantha, K Yogendra, N Madhusudhana

{"title":"Sustainable synthesis of benzimidazole-based Schiff base using reusable CaAl2O4 nanophosphors catalyst: Insights into metal(II) complexes and DNA interactions.","authors":"M Manjunath, F H Sujata, A H Shridhara, B Vinay Kumar, K Prashantha, K Yogendra, N Madhusudhana","doi":"10.1080/15257770.2025.2451375","DOIUrl":"10.1080/15257770.2025.2451375","url":null,"abstract":"This article presents a new and facile method for the synthesis of Schiff base compounds with a benzimidazole group using a low-cost and reusable calcium aluminate nanophosphorus catalyst (CaAl2O4). This approach avoids harmful solvents and reactants, supporting a more environmentally friendly synthesis process. The catalyst maintained its activity and heterogeneity over four cycles with minimal loss of efficiency. The synthesis process was straightforward and eliminated the need for column chromatography. The Schiff base ligand (HL=(E)-N-((6-(thiophen-2-yl)pyridin-2-yl)methylene)-1H-benzo[d]imidazol-2-amine)) was synthesized by the reaction of 6-(thiophen-2-yl)pyridine-2-carbaldehyde with 1H-benzimidazole-2-amine. Subsequently, metal(II) complexes of Co(II), Ni(II), and Cu(II) were prepared using this ligand. Structural analysis of both the ligand and its metal complexes was carried out using various physicochemical and spectroscopic methods. Ni(II) and Co(II) complexes were found to adopt an octahedral geometry, while the Cu(II) complex exhibited a square-planar structure. Binding studies with calf thymus DNA (CT-DNA) at pH 7.2 were performed using UV-visible spectroscopy, viscosity measurements, and thermal denaturation studies and showed that the metal complexes intercalate into the DNA and produced a distinct binding pattern. Molecular docking simulations with AutoDock Vina provided insights into the interaction of these complexes with the B-DNA dodecamer. Furthermore, the ligand and its metal complexes showed UV-visible photonuclease activity against pUC19 DNA. Agarose gel electrophoresis showed that the metal complexes exhibit photoinduced nuclease activity, confirming their ability to cleave DNA upon exposure to light.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"238-260"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano calcium zincate-assisted synthesis of benzo[d]thiazol-2-yl phenylisoxazoles: quantum computational, in silico molecular docking simulations and DNA interaction. 纳米锌酸钙辅助合成苯并[d]噻唑-2-基苯基异恶唑：量子计算、硅分子对接模拟和DNA相互作用。

IF 1.3 4区生物学

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2026-01-01 Epub Date: 2025-03-06 DOI: 10.1080/15257770.2025.2473442

A K Smitha, V Srinivasa Murthy, B Vinay Kumar, M Sennappan, A H Shridhar, Lohit Naik, K Yogendra, N Madhusudhana

{"title":"Nano calcium zincate-assisted synthesis of benzo[d]thiazol-2-yl phenylisoxazoles: quantum computational, in silico molecular docking simulations and DNA interaction.","authors":"A K Smitha, V Srinivasa Murthy, B Vinay Kumar, M Sennappan, A H Shridhar, Lohit Naik, K Yogendra, N Madhusudhana","doi":"10.1080/15257770.2025.2473442","DOIUrl":"10.1080/15257770.2025.2473442","url":null,"abstract":"This study introduces a new and simple method for the synthesis of a series of 3-(benzo[d]thiazol-2-yl)-5-phenylisoxazole derivatives 3(a-f), and examines its potential interactions with DNA. The synthesis includes the reaction of 2-aminobenzenethiol (1) with a variety of substituted 5-phenylisoxazole-3-carbaldehydes 2(a-f) in the presence of a cost-effective and reusable nanocatalyst, Calcium-Zincate (CaZnO2). The CaZnO2 catalyst showed a consistent and long-lasting catalytic activity over several reaction cycles and retained its unique heterogeneous properties. The resulting compounds were characterized in detail using various spectroscopic and analytical techniques in order to confirm their structures. In addition, the interaction of these synthesized compounds with calf thymus-DNA (CT-DNA) using absorption spectroscopy and viscosity measurements was assessed. In silico docking studies were performed to predict their binding affinity with human DNA (PDB ID: 1G3X). The compounds were further analyzed using the Density Functional Theory (DFT) with the B3LYP functional and the 6-31 G(d) basis set in chloroform, with the results aligning closely with the experimental findings. Furthermore, the compounds ability to cleave PUC19 DNA was assessed, along with their photoinduced nuclease activity under UV-visible light, confirmed by photo-induced cleavage assays.","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"261-286"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0