Prognostic value of miR-378c in hepatocellular carcinoma and its regulatory effect on tumor progression.

IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuanjie Bao, Haoxiang Zhu
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引用次数: 0

Abstract

Objective: This study aimed to explore the diagnostic and prognostic value of miR-378c in hepatocellular carcinoma (HCC) patients.

Methods: This study included 97 HCC patients, 84 cirrhosis patients and 80 healthy volunteers. Serum miR-378c of all subjects and HCC cell lines was detected by qRT-PCR, and ROC curves were plotted to assess the clinical diagnostic value of miR-378c for HCC. The prognostic performance of miR-378c in HCC was assessed using the Kaplan-Meyer method and COX regression analysis. CCK-8 test for proliferation of HCC cell lines. The migration and invasion of HCC cell lines were measured by Transwell assay. Bioinformatics analysis was employed to analyze the possible target genes of miR-378c.

Results: Serum miR-378c were remarkably lower in HCC patients than in cirrhosis patients and healthy controls (p < 0.001). ROC curves indicated that serum miR-378c could effectively distinguish HCC patients from healthy controls and cirrhotic patients. Among HCC patients, those with high miR-378c expression had higher cumulative survival (p = 0.001), and COX analysis identified miR-378c as an independent prognostic biomarker for HCC. Overexpression of miR-378c significantly inhibited the proliferation, migration and invasion of MHCC97H and HepG2 cells (p < 0.01). Bioinformatics analysis of miR-378c target genes revealed that miR-378c target genes were enriched in tumor-associated pathways.

Conclusion: Serum miR-378c expression is decreased in HCC patients and strongly connected with poor prognosis. As a potential diagnostic and prognostic biomarker for HCC patients, it may provide new insights into the diagnosis and prognosis of HCC.

miR-378c在肝癌中的预后价值及其对肿瘤进展的调控作用。
目的:本研究旨在探讨miR-378c在肝细胞癌(HCC)患者中的诊断和预后价值。方法:本研究纳入97例HCC患者、84例肝硬化患者和80例健康志愿者。采用qRT-PCR检测所有受试者及HCC细胞系的血清miR-378c,绘制ROC曲线,评估miR-378c对HCC的临床诊断价值。采用Kaplan-Meyer方法和COX回归分析评估miR-378c在HCC中的预后性能。CCK-8检测肝癌细胞系增殖。Transwell法检测肝癌细胞株的迁移和侵袭。采用生物信息学分析分析miR-378c可能的靶基因。结果:HCC患者血清miR-378c明显低于肝硬化患者和健康对照组(p p = 0.001), COX分析确定miR-378c是HCC的独立预后生物标志物。过表达miR-378c可显著抑制MHCC97H和HepG2细胞的增殖、迁移和侵袭(p结论:HCC患者血清miR-378c表达降低,与预后不良密切相关。作为HCC患者潜在的诊断和预后生物标志物,它可能为HCC的诊断和预后提供新的见解。
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来源期刊
Nucleosides, Nucleotides & Nucleic Acids
Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学
CiteScore
2.60
自引率
7.70%
发文量
91
审稿时长
6 months
期刊介绍: Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.
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