miR-508-5p regulates macrophage polarization via targeting TSGA10 to promote malignant behavior in esophageal cancer cells.

IF 1.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-05-11 DOI:10.1080/15257770.2025.2491561

Yuan Zhu, Zuojun Fu, Tianjiao Duan, Jing Wang, Lingjuan Zhang, Guisheng Liu, Xueyan Guo, Rong Zhang

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引用次数: 0

Abstract

Background: Esophageal cancer (EC) is among the deadliest malignancies in humans, with various miRNAs shown to regulate its progression by targeting distinct genes. miR-508-5p was identified as being linked to the malignant behavior of various tumors. Nevertheless, the precise role and mechanism of miR-508-5p in esophageal cancer (EC) remain ambiguous.

Objective: This investigation focuses on the role and mechanism of the miR-508-5p/TSGA10 axis in the progression of EC.

Methods: The expression of miR-508-5p and TSGA10 in EC cell lines was evaluated using quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Cell transfection techniques were used to knock down miR-508-5p and observe its effects on cell proliferation, migration, invasion, and apoptosis. A dual-luciferase reporter gene assay was conducted to verify the targeting relationship of miR-508-5p with TSGA10. Co-culture studies were undertaken to examine the regulatory effect of the miR-508-5p/TSGA10 axis on the polarization state of tumor-associated macrophages (TAMs) and the malignant behavior of EC cells.

Results: The expression of miR-508-5p was significantly elevated in EC cells. Knocking down miR-508-5p curbed cell proliferation, migration, and invasion while promoting apoptosis. TSGA10 was validated as a primary target gene of miR-508-5p. miR-508-5p knockdown could inhibit the M2 polarization of TAMs by upregulating TSGA10, thereby suppressing the tumorigenic behavior of EC cells.

Conclusion: miR-508-5p promotes the M2 polarization of TAMs and enhances the malignant behavior of EC cells by inhibiting TSGA10.

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miR-508-5p通过靶向TSGA10调控巨噬细胞极化，促进食管癌细胞的恶性行为。

背景：食管癌（EC）是人类最致命的恶性肿瘤之一，各种mirna通过靶向不同的基因来调节其进展。miR-508-5p被认为与多种肿瘤的恶性行为有关。然而，miR-508-5p在食管癌（EC）中的确切作用和机制尚不清楚。目的：探讨miR-508-5p/TSGA10轴在EC进展中的作用及机制。方法：采用实时荧光定量pcr （quantitative Real-Time Polymerase Chain Reaction, qRT-PCR）检测miR-508-5p和TSGA10在EC细胞系中的表达。采用细胞转染技术敲低miR-508-5p，观察其对细胞增殖、迁移、侵袭和凋亡的影响。通过双荧光素酶报告基因实验验证miR-508-5p与TSGA10的靶向关系。共培养研究旨在检测miR-508-5p/TSGA10轴对肿瘤相关巨噬细胞（tumor-associated macrophages, tam）极化状态和EC细胞恶性行为的调控作用。结果：miR-508-5p在EC细胞中表达明显升高。下调miR-508-5p抑制细胞增殖、迁移和侵袭，同时促进细胞凋亡。TSGA10被证实是miR-508-5p的主要靶基因。miR-508-5p敲低可通过上调TSGA10抑制tam的M2极化，从而抑制EC细胞的致瘤行为。结论：miR-508-5p通过抑制TSGA10促进TAMs的M2极化，增强EC细胞的恶性行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

6 months

期刊介绍： Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.