MiR-34a rs2666433 and cognitive function in major depressive disorder: a clinical correlation analysis.

IF 1.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-06-02 DOI:10.1080/15257770.2025.2511106

Ning Li, Xiaochuan Zhao

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Abstract

Background: Genetic factors play a crucial role in the development of major depressive disorder (MDD).

Objectives: This study aimed to investigate the association between the microRNA (miR)-34a rs266643 polymorphism and MDD, as well as its impact on cognitive function.

Materials and methods: Clinical data and blood samples were collected from 302 MDD patients and 306 healthy controls who met the predefined inclusion and exclusion criteria. The severity of MDD in patients was assessed using the Hamilton Rating Scale for Depression (HRSD). Cognitive function in MDD patients was evaluated using the Mini-Mental State Examination (MMSE), Stroop Color-Word Test (Stroop-C and Stroop-CW), and Montreal Cognitive Assessment (MoCA). Gene typing was performed using the Sanger sequencing method, while the relative expression level of miR-34a was quantified by RT-qPCR.

Results: The MDD group exhibited a significantly higher miR-34a expression fold change compared to the control group (p < 0.001). Among the genotypes, the AA genotype demonstrated the highest expression, followed by GA, with both being significantly greater than GG. The expression of miR-34a was positively correlated with HRSD, Stroop-C, and Stroop-CW scores but negatively correlated with MMSE and MoCA scores (p < 0.001). Carrying the A allele (OR = 1.468, p = 0.002) or the AA genotype (OR = 2.382, p = 0.001) was associated with an increased risk of MDD. Furthermore, patients with the AA genotype exhibited significantly poorer cognitive function compared to other genotypes.

Conclusion: The gene polymorphism of miR-34a rs2666433 was significantly associated with the severity of MDD as well as cognitive function.

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MiR-34a rs2666433与重度抑郁症认知功能的临床相关性分析

背景：遗传因素在重度抑郁症（MDD）的发展中起着至关重要的作用。目的：本研究旨在探讨microRNA (miR)-34a rs266643多态性与MDD的关系及其对认知功能的影响。材料与方法：收集302例重度抑郁症患者和306例符合纳入和排除标准的健康对照者的临床资料和血液样本。使用汉密尔顿抑郁量表（HRSD）评估患者重度抑郁症的严重程度。采用简易精神状态检查（MMSE）、Stroop颜色-单词测试（Stroop- c和Stroop- cw）和蒙特利尔认知评估（MoCA）对MDD患者的认知功能进行评估。采用Sanger测序法进行基因分型，RT-qPCR定量miR-34a的相对表达量。结果：MDD组miR-34a表达倍数变化明显高于对照组（p p p = 0.002）， AA基因型（or = 2.382, p = 0.001）与MDD风险增加相关。此外，与其他基因型相比，AA基因型患者表现出明显较差的认知功能。结论：miR-34a rs2666433基因多态性与重度抑郁症严重程度及认知功能显著相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

6 months

期刊介绍： Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.