NMR in Biomedicine最新文献

{"title":"Automatic Identification of Potential Cellular Metabolites for Untargeted NMR Metabolomics.","authors":"Jiashang Chen, Angela Rao, Rajshree Ghosh Biswas, Ella J Zhang, Jonathan Xin Zhou, Evan Zhang, Zuzanna Kobus, Marta Kobus, Li Su, David C Christiani, David S Wishart, Leo L Cheng","doi":"10.1002/nbm.70131","DOIUrl":"10.1002/nbm.70131","url":null,"abstract":"<p><p>An organism's metabolic profile provides vital information pertaining to its physiology or pathology. To monitor these biochemical changes, Nuclear Magnetic Resonance (NMR) spectroscopy has found success in non-invasively observing metabolite changes within intact samples in an untargeted manner. However, biological samples are chemically complex, comprised of many different constituents (amino acids, carbohydrates, and lipids) at varying concentrations depending on physiological and pathological conditions. Due to the narrow spectral window of proton NMR, compound resonance frequencies can often overlap, making the identification and monitoring of metabolites difficult and time consuming, particularly when dealing with large numbers of samples. Here, we introduce a Python program (ROIAL-NMR) to systematically identify potential metabolites from defined proton NMR spectral regions-of-interest (ROIs), which are identified from complex biological samples (i.e., human serum, saliva, sweat, urine, CSF, and tissues) using the Human Metabolome Database (HMDB) as a reference platform. Briefly, for disease-versus-control studies, the program considers disease types and utilizes study-defined ROIs together with their differing intensity levels, according to sample types, in differentiating disease from control to propose potential metabolites represented by these ROIs in an output table. In this report, we illustrate the utility of the program with one of our recent studies, where we measured proton NMR spectra of serum samples taken from lung cancer (LC) patients, with and without Alzheimer's disease and related dementia (ADRD). The program successfully identified 88 metabolites, with 66 differentiating LC from control patients, and 80 distinguishing LC patients with ADRD from those without ADRD to provide important information regarding pathophysiology in complex biological samples.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 10","pages":"e70131"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated 3D qCEST of the Spine in a Porcine Model Using MR Multitasking at 3T.","authors":"Karandeep Cheema, Dante Rigo De Righi, Chushu Shen, Hsu-Lei Lee, Giselle Kaneda, Jacob Wechsler, Melissa Chavez, Pablo Avalos, Candace Floyd, Wafa Tawackoli, Yibin Xie, Anthony G Christodoulou, Dmitriy Sheyn, Debiao Li","doi":"10.1002/nbm.70122","DOIUrl":"10.1002/nbm.70122","url":null,"abstract":"<p><p>To assess lower back pain using quantitative chemical exchange saturation transfer (qCEST) imaging in a porcine model by comparing exchange rate maps obtained from multitasking qCEST with conventional qCEST. Use a permuted random forest (PRF) model trained on CEST-derived magnetization transfer ratio (MTR) and exchange rate (k_sw) features to predict Glasgow pain scores. Six Yucatan minipigs were scanned at baseline and at four post-injury time points (weeks 4, 8, 12, and 16) following intervertebral disc injury. Conventional qCEST imaging was performed at four B1 powers using a two-dimensional reduced field of view turbo spin-echo (TSE) sequence, with a total acquisition time of 24 min per slice. Multitasking steady-state (SS) CEST imaging was performed with pulsed saturation to achieve a steady state, acquiring 32 slices at 59 offsets for 4 B1 powers in 36 min. Exchange rate maps were generated using omega plot analysis, and CEST images were analyzed using a multi-pool fitting model to produce MTR and k_sw maps. Permuted random forest (PRF) model was trained on MTR and k_sw values to predict pain scores. Modic changes were assessed using T2-weighted MR images. The Pearson correlation coefficient between exchange rate maps from multitasking qCEST and conventional qCEST was 0.82, demonstrating strong agreement. The 3D qCEST (SS-CEST) technique effectively differentiated between healthy and injured discs, with injured discs exhibiting significantly higher k_sw values. Using MTR and k_sw, the PRF model achieved 80% accuracy in predicting pain scores disc-by-disc, outperforming the correlation with Modic changes (r = 0.45, p < 0.05); with a Cohen's Kappa of 0.4. 3D steady-state qCEST with whole-spine coverage can be done at 3T within 32 min using MR Multitasking (acceleration factor of 22), and qCEST-derived biomarkers (MTR and k_sw) can predict pain scores with an accuracy of 80%.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 9","pages":"e70122"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved Strength Prediction Combining MRI Biomarkers of Muscle Quantity and Quality.","authors":"Valentina Mazzoli, Yael Vainberg, Mary E Hall, Marco Barbieri, Jessica Asay, Julie Muccini, Jarret Rosenberg, Feliks Kogan, Scott Delp, Garry E Gold","doi":"10.1002/nbm.70112","DOIUrl":"10.1002/nbm.70112","url":null,"abstract":"<p><p>Muscle strength declines with aging at a faster rate compared with muscle mass, suggesting that not only muscle quantity but also muscle quality and architecture are age-dependent. This study tested the hypothesis that quantitative MRI (qMRI)-derived biomarkers of muscle quality (fractional anisotropy [FA], radial diffusivity [RD], axial diffusivity [AD], fat fraction [FF], and T₂ relaxation time) and architecture (fascicle length) could improve the prediction of skeletal muscle strength over muscle mass alone. We recruited 24 adults (12 female, age range 30-79 years). Muscle mass was estimated as the volume and cross-sectional area (CSA) of the quadriceps. FA, RD, and AD parameters, together with fascicle length for the rectus femoris (RF) and vastus lateralis (VL), were derived from diffusion tensor imaging (DTI), and muscle-T₂ was calculated from a multi-echo spin echo sequence. FF was determined using the Dixon approach. CSA values were combined with FF to calculate the lean CSA. Isometric, eccentric, and concentric knee extension torques were measured for the left and right leg using an isokinetic dynamometer. The univariable assessment of torque was performed using a linear regression. The statistical significance of adding qMRI parameters to the torque prediction models was tested using a mixed-effect regression. The best univariable predictor of isometric, eccentric, and concentric torque was lean CSA. Adding FA, RF fascicle length, and VL fascicle length to the model improved the prediction of concentric torque compared with CSA alone. The addition of FA, T₂, RD, RF fascicle length, and VL fascicle length improved the prediction of eccentric torque over CSA alone. The addition of FF was not significant within the model. Our results confirmed the hypothesis that the inclusion of qMRI parameters of muscle composition and architecture leads to higher R² coefficients for the prediction of muscle strength compared with models solely based on muscle quantity. These observations support the utility of qMRI for future research on sarcopenia prediction and management.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 9","pages":"e70112"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved IVIM Imaging in Adolescent Crohn's Disease Using Dual-Echo EPI Distortion and Motion Correction.","authors":"Cemre Ariyurek, Lina Lu, Georgios Antonios Sideris, Valentina Valencia Ferrer, Liam Timms, Serge Didenko Vasylechko, Onur Afacan, Sila Kurugol","doi":"10.1002/nbm.70117","DOIUrl":"10.1002/nbm.70117","url":null,"abstract":"<p><p>Diffusion-weighted imaging (DWI) during MR enterography helps identify bowel inflammation in Crohn's disease (CD). However, image quality is compromised by geometric distortions from B₀ field variations and physiological motion, making it challenging for radiologists to correlate findings between DWI and structural images. Traditional correction methods using reversed polarity scans are ineffective due to motion between acquisitions, which limits accurate estimation of intravoxel incoherent motion (IVIM) parameters. We propose a dual-echo echo-planar imaging (EPI) method that retrospectively corrects both geometric distortions and motion in 3T bowel DWI by accounting for field changes during peristalsis and breathing. We added a 5- to 7-min dual-echo EPI DW sequence (eight b-values, six directions) to the clinical MR enterography protocol of 21 patients with suspected CD at 3T MRI. Distortion correction was applied based on dynamically estimated fields from dual-echo DWI, followed by intra-volume registration between odd-even slices and inter-volume registration for motion correction. Two experienced board-certified radiologists evaluated the severity of the disease using simplified magnetic resonance index of activity (MaRIA) scores. Based on their consensus scores, patients were categorized into three groups: no active disease (MaRIA score = 0), active disease (MaRIA score = 1-2), and severe disease (MaRIA score = 3-5). The proposed DWI correction pipeline improved DWI/T₂-weighted image Dice similarity from 0.73 to 0.89, enabling better correlation of findings between structural and DW-MR images and enhancing DWI's clinical value. Corrected IVIM parameters showed stronger correlations with MaRIA scores (D: ρ = -0.93; f: ρ = -0.94, p < 0.001) compared to uncorrected parameters (D: ρ = -0.68, p = 0.001; f: ρ = -0.35, p = 0.118). Diagnostic sensitivity increased from 0.44 to 0.89, while parameter uncertainty decreased from 35.58% to 19.31% for D and 63.48% to 40.40% for f (p < 0.001). These improvements strengthen quantitative IVIM imaging for CD assessment, potentially reducing reliance on contrast imaging while offering enhanced tissue perfusion and diffusion insights.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 9","pages":"e70117"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12392371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of Deuterium Metabolic Magnetic Resonance Spectroscopy for the Investigation of Ischemia and Reperfusion in Rat Brain Slices Perfused Ex Vivo.","authors":"Sarah Abendanan, David Shaul, J Moshe Gomori, Rachel Katz-Brull","doi":"10.1002/nbm.70115","DOIUrl":"10.1002/nbm.70115","url":null,"abstract":"<p><p>Investigating glucose metabolism in the brain using [6,6-²H₂]glucose (²H₂-Glc) and deuterium-based NMR spectroscopy has shown promise for noninvasive monitoring of the fate of this labeled compound. This approach has already been applied in vivo in small animals and human subjects. A model of perfused rat brain slices recently showed promise for the investigation of the metabolic consequences of acute ischemic stroke, which is a significant cause of death and morbidity worldwide. The current study aimed to implement the deuterium-based glucose metabolism monitoring approach to study the metabolic consequences of ischemia and reperfusion in the rat brain ex vivo. In agreement with previous studies, we found that deuterated lactate (²H₂-Lac) was immediately formed in the brain upon administration of ²H₂-Glc to the perfusion medium. This metabolite remained the predominant metabolic fate observed in the ²H-NMR spectra. Upon perfusion arrest, ²H₂-Lac quickly built up to the same amount of ²H₂-Glc eliminated from the medium engulfing the slices, reaching fivefold to sixfold its baseline level (n = 6, three animals, and two ischemic conditions in each). Upon reperfusion, ²H₂-Lac decreased to its level before the ischemic condition, and ²H₂-Glc returned to its baseline. ²H₂-Lac washout to the medium amounted to 2.2% of the ²H₂-Lac signal associated with the slices after about 5 h of perfusion with ²H₂-Glc, suggesting that the ²H₂-Lac signal observed during the experiments was predominantly intracellular. These results demonstrate the utility of ²H₂-Glc and ²H-NMR in monitoring the consequences of ischemia and reperfusion in the perfused rat brain slices model.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 9","pages":"e70115"},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12358337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volumetric and Diffusion Tensor Imaging Abnormalities Are Associated With Behavioral Changes Post-Concussion in a Youth Pig Model of Mild Traumatic Brain Injury.","authors":"Islam Sanjida, Netzley Alesa, Li Chenyang, Zhang Jiangyang, Dávila-Montero Bianca, Vazquez Ana, Subbaiah Shaun, Meoded Avner, Munoz Kirk, Colbath Aimee, Huang Jie, Mejia-Alvarez Ricardo, Manfredi Jane, Pelled Galit","doi":"10.1002/nbm.70074","DOIUrl":"10.1002/nbm.70074","url":null,"abstract":"<p><p>Mild traumatic brain injury (mTBI) caused by sports-related incidents in children and youth often leads to prolonged cognitive impairments but remains difficult to diagnose. In order to identify clinically relevant imaging and behavioral biomarkers associated concussion, a closed-head mTBI was induced in adolescent pigs. Twelve (n = 4 male and n = 8 female), 16-week old Yucatan pigs were tested; n = 6 received mTBI and n = 6 received a sham procedure. T1-weighted imaging was used to assess volumetric alterations in different regions of the brain and diffusion tensor imaging (DTI) to examine microstructural damage in white matter. The pigs were imaged at 1- and 3-month post-injury. Neuropsychological screening for executive function and anxiety were performed before and in the months after the injury. The volumetric analysis showed significant longitudinal changes in pigs with mTBI compared with sham, which may be attributed to swelling and neuroinflammation. Fractional anisotropy (FA) values derived from DTI images demonstrated a 21% increase in corpus callosum from 1 to 3 months in mTBI pigs, which is significantly higher than in sham pigs (4.8%). Additionally, comparisons of the left and right internal capsules revealed a decrease in FA in the right internal capsule for mTBI pigs, which may indicate demyelination. The neuroimaging results suggest that the injury had disrupted the maturation of white and gray matter in the developing brain. Behavioral testing showed that compare to sham pigs, mTBI pigs exhibited 23% increased activity in open field tests, 35% incraesed escape attempts, along with a 65% decrease in interaction with the novel object, suggesting possible memory impairments and cognitive deficits. The correlation analysis showed an associations between volumetric features and behavioral metrics. Furthermore, a machine learning model, which integrated FA, volumetric features and behavioral test metrics, achieved 67% accuracy, indicating its potential to differentiate the two groups. Thus, the imaging biomarkers were indicative of long-term behavioral impairments and could be crucial to the clinical management of concussion in youth.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 7","pages":"e70074"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-Tuning Deep Learning Model for Quantitative Knee Joint Mapping With MR Fingerprinting and Its Comparison to Dictionary Matching Method: Fine-Tuning Deep Learning Model for Quantitative MRF.","authors":"Xiaoxia Zhang, Hector L de Moura, Anmol Monga, Marcelo V W Zibetti, Ravinder R Regatte","doi":"10.1002/nbm.70045","DOIUrl":"10.1002/nbm.70045","url":null,"abstract":"<p><p>Magnetic resonance fingerprinting (MRF), as an emerging versatile and noninvasive imaging technique, provides simultaneous quantification of multiple quantitative MRI parameters, which have been used to detect changes in cartilage composition and structure in osteoarthritis. Deep learning (DL)-based methods for quantification mapping in MRF overcome the memory constraints and offer faster processing compared to the conventional dictionary matching (DM) method. However, limited attention has been given to the fine-tuning of neural networks (NNs) in DL and fair comparison with DM. In this study, we investigate the impact of training parameter choices on NN performance and compare the fine-tuned NN with DM for multiparametric mapping in MRF. Our approach includes optimizing NN hyperparameters, analyzing the singular value decomposition (SVD) components of MRF data, and optimization of the DM method. We conducted experiments on synthetic data, the NIST/ISMRM MRI system phantom with ground truth, and in vivo knee data from 14 healthy volunteers. The results demonstrate the critical importance of selecting appropriate training parameters, as these significantly affect NN performance. The findings also show that NNs improve the accuracy and robustness of T₁, T₂, and T_1ρ mappings compared to DM in synthetic datasets. For in vivo knee data, the NN achieved comparable results for T₁, with slightly lower T₂ and slightly higher T_1ρ measurements compared to DM. In conclusion, the fine-tuned NN can be used to increase accuracy and robustness for multiparametric quantitative mapping from MRF of the knee joint.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 6","pages":"e70045"},"PeriodicalIF":2.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coil Combination Using OpTIMUS Results in Improved Signal-to-Noise Ratios of In Vivo MR Spectra Acquired at 7 T.","authors":"Eva Martinez Luque, Dongsuk Sung, Benjamin B Risk, Rachel M Goldberg, Candace C Fleischer","doi":"10.1002/nbm.70044","DOIUrl":"10.1002/nbm.70044","url":null,"abstract":"<p><p>Magnetic resonance spectroscopy (MRS) enables noninvasive quantification of metabolites, but its utility in vivo can be limited by low signal-to-noise ratios (SNRs) and long acquisition times. The use of ultrahigh-field (UHF) strengths (> 3 T) combined with multichannel phased receive arrays can improve spectral SNR. A crucial step in the use of multichannel arrays is the combination of spectra acquired from individual coil channels. We previously developed a coil combination method at 3 T, optimized truncation to integrate multichannel MRS data using rank-R singular value decomposition (OpTIMUS), which uses noise-whitened windowed spectra and iterative rank-R singular value decomposition (SVD) to combine multichannel MRS data. Here, we evaluated OpTIMUS for combination of MR spectra acquired using a multichannel phased array at 7 T and compared spectral SNR and metabolite quantification with spectra combined using whitened SVD (WSVD), signal/noise squared (S/N²), and the Brown method. Data were acquired from 14 healthy volunteers, including five with data acquired at both 3 and 7 T, and from nine people living with HIV. Spectra combined using OpTIMUS resulted in a higher SNR compared to the three other methods, consistent with our prior results at 3 T. With half the number of averages (N = 32), spectra combined with OpTIMUS had higher SNR compared to spectra using the Brown method with 64 averages. Additionally, spectra combined using OpTIMUS at 7 T were compared to spectra acquired at 3 T with the same number of averages (N = 64) or matched acquisition times (N = 110 averages), and spectral fitting was consistently improved at 7 T even when comparable SNR was obtained at 3 T. The ability to increase SNR and maintain spectral quality by optimizing spectral coil combination has the potential to reduce scan time, a key challenge for routine clinical use of MRS.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 6","pages":"e70044"},"PeriodicalIF":2.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffusion Tensor Imaging of Rat Rotator Cuff Muscle With Histopathological Correlation: An Exploratory Study.","authors":"James Lo, David B Berry, Qingbo Tang, Xin Cheng, Marco Toto-Brocchi, Jiang Du, Samuel R Ward, Yajun Ma, Eric Y Chang","doi":"10.1002/nbm.70058","DOIUrl":"10.1002/nbm.70058","url":null,"abstract":"<p><p>The purpose of this exploratory study was to quantify the relationship between scalar-based measures of diffusion tensor imaging (DTI) and histologically derived microstructural measurements in precisely colocalized rat rotator cuff muscle tissue and to compare the results when imaged at 0.25- and 0.5-mm isotropic resolutions. Four Lewis rats subject to a unilateral chronic massive rotator cuff tear model were evaluated on a 3-T preclinical MRI scanner using spin echo DTI sequences at 0.25- and 0.5-mm isotropic resolutions, and histology was subsequently performed. Fractional anisotropy (FA), mean diffusivity (MD), and radial diffusivity (RD) were calculated. Whole muscle myofiber boundary segmentation was performed, and muscle fiber diameter and cross-sectional area were calculated on slides that passed rigorous histologic quality control. Scatter plots were generated on a pixel-by-pixel basis from meticulously colocalized DTI and histology data. Pearson's correlations were performed. Twenty-two distinct supraspinatus and infraspinatus muscle locations from two rats were included. Negligible correlations were found between DTI metrics, including FA, MD, and RD, and histological measurements, including muscle fiber diameters and cross-sectional areas. Using the most commonly employed spin echo DTI sequences with intermediate diffusion times, there may be negligible sensitivity to direct measures of muscle tissue microstructure. Our findings underscore the need for further research with optimized imaging parameters to enhance our knowledge regarding the capability of DTI to determine important features of muscle microstructure.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 6","pages":"e70058"},"PeriodicalIF":2.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproducibility of the Determination of ¹³C-Labeling of Glutamate and Glutamine in the Human Brain Using selPOCE-MRS at 7 T Upon [U-¹³C]-Labeled Glucose Infusion.","authors":"Narjes Ahmadian, Sarah M Jacobs, Mark Gosselink, Wybe J M van der Kemp, Hans Hoogduin, Anastasia Coppoli, Graeme F Mason, Robin A de Graaf, Helia Norouzizadeh, Chantal Mahon, Pieter van Eijsden, Renger Tiessen, Dirk Cerneus, Corin O Miller, Inge De Lepeleire, Anthony S Basile, Dennis W J Klomp, Jeanine J Prompers, Evita C Wiegers","doi":"10.1002/nbm.70026","DOIUrl":"https://doi.org/10.1002/nbm.70026","url":null,"abstract":"<p><p>Glutamate (Glu) is the major excitatory neurotransmitter in the central nervous system. The measurement of Glu/glutamine (Gln) neurotransmitters in the brain provides valuable insights into the dynamic aspects of neuroenergetics and neurotransmitter cycles and can be accomplished through the detection of ¹³C-labeling of Glu and Gln during the administration of ¹³C-labeled glucose. Our goal is to evaluate the reproducibility of selective proton-observed, carbon-edited (selPOCE) MRS at 7 T for the detection of ¹³C-labeled Glu and Gln in the human brain. Data of three healthy participants, who were scanned twice at 7 T while undergoing [U-¹³C]-glucose infusion for 120 min, were used to detect ¹³C-labeled Glu and Gln in the brain, using selPOCE-STEAM-MRS. There was a rapid increase of plasma glucose ¹³C fractional enrichment (FE) during the first 20 min of infusion, followed by a steady state of plasma glucose ¹³C FE until the end of the [U-¹³C]-glucose infusion. The time courses of ¹³C-labeling of Glu and Gln were similar for test/retest. The test/retest variability was 15.8% for ¹³C-Glu and 33.3% for ¹³C-Gln. Knowing the variability of these readings using selPOCE-STEAM-MRS can inform the application to future studies on disease-specific alterations in Glu/Gln cycling.</p>","PeriodicalId":19309,"journal":{"name":"NMR in Biomedicine","volume":"38 5","pages":"e70026"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11981885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}